MD07G1265900.v1.1

CEN-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007031
Physical Location & Seq
Forward (+)
33427173 .. 33428512
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1265900.v1.1.491

Sequence Viewer

Length: 525 bp
ATGTCAAGGATGATGGAGCCACTAACTCTTGGAAGAGTGATAGGAGAGGTTGTGGACATCTTCACCCCAACTGTGAACCTGAATGTAGTTTACAACCCAAACAGACAAGTTGCTAATGGACATGAGCTCATGCCTTCTGTCATTGCTGCTAAACCTCGCGTCGAGATCGGAGGTGAAGATATGAGGACTGCTTATACCTTAATAATGACAGACCCAGATTTTCCAAGCCCCAGCGAACCATACCTGAGAGAACATCTCCACTGGATGGTCACAGACATTCCTGGCACCACTGATGTCTCATTTGGAAAAGAGATTGTCGAGTATGAAATTCCACGGCCGGTCGTAGGCATTCACAGGTACGTATTCCTGCTATTCAAGCAGAGAGGAAGACAAACAGTGAGAGCTCCAGCTTCCAGAGACAATTTCAATACCAGAATATTCTCGCAGGAGAATGGTCTCGGGCTGCCTGTGGCTGCCGTCTACTTCAATGCACAACGGGCAACTGCTGCTAGAAGAAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009791 GO:0009908 GO:0010228 GO:0022414 GO:0032501 GO:0032502 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0090567
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBP

Protein Analysis

175

Amino Acids

19.69

Weight (kDa)

8.85

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PBP PF01161 52 - 161 7.9e-13 Phosphatidylethanolamine-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 265
AccI GTMKAC 1 cut(s) 480
AccII CGCG 1 cut(s) 159
AcoI YGGCCR 1 cut(s) 335
AcsI RAATTY 1 cut(s) 327
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 265, 344
AgsI TTSAA 3 cut(s) 376, 427, 487
AjnI CCWGG 1 cut(s) 280
AluBI AGCT 3 cut(s) 127, 404, 410
AluI AGCT 3 cut(s) 127, 404, 410
Alw21I GWGCWC 2 cut(s) 129, 406
Alw26I GTCTC 3 cut(s) 301, 411, 461
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 1 cut(s) 335
ApeKI GCWGC 4 cut(s) 146, 463, 473, 506
ApoI RAATTY 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 55, 185
AvaI CYCGRG 1 cut(s) 458
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 2 cut(s) 129, 406
BbsI GAAGAC 1 cut(s) 394
Bbv12I GWGCWC 2 cut(s) 129, 406
BbvI GCAGC 4 cut(s) 133, 450, 460, 493
BccI CCATC 2 cut(s) 7, 259
BceAI ACGGC 2 cut(s) 350, 461
BciT130I CCWGG 1 cut(s) 282
BcoDI GTCTC 3 cut(s) 301, 411, 461
BfaI CTAG 1 cut(s) 510
BisI GCNGC 4 cut(s) 147, 464, 474, 507
BlsI GCNGC 4 cut(s) 148, 465, 475, 508
Bme1390I CCNGG 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 458
BmiI GGNNCC 2 cut(s) 18, 286
BmrFI CCNGG 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 394
BplI GAGNNNNNCTC 2 cut(s) 240, 272
BpmI CTGGAG 1 cut(s) 390
BsaAI YACGTR 1 cut(s) 361
BsaI GGTCTC 1 cut(s) 461
BsaJI CCNNGG 1 cut(s) 332
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 344
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 141
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 332
BseGI GGATG 2 cut(s) 15, 270
BseLI CCNNNNNNNGG 2 cut(s) 265, 344
BseMI GCAATG 1 cut(s) 141
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 1 cut(s) 266
BseX3I CGGCCG 1 cut(s) 335
BseXI GCAGC 4 cut(s) 133, 450, 460, 493
BseYI CCCAGC 1 cut(s) 230
Bsh1236I CGCG 1 cut(s) 159
Bsh1285I CGRYCG 2 cut(s) 338, 342
BshFI GGCC 1 cut(s) 337
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 2 cut(s) 338, 342
BsiHKAI GWGCWC 2 cut(s) 129, 406
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 1 cut(s) 338
BslI CCNNNNNNNGG 2 cut(s) 265, 344
BsmAI GTCTC 3 cut(s) 301, 411, 461
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 337
Bso31I GGTCTC 1 cut(s) 461
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 129, 406
Bsp143I GATC 1 cut(s) 165
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 1 cut(s) 237
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 2 cut(s) 18, 286
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 141
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 332
BssMI GATC 1 cut(s) 165
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 73, 397
Bst6I CTCTTC 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 1 cut(s) 332
BstF5I GGATG 2 cut(s) 15, 270
BstFNI CGCG 1 cut(s) 159
BstKTI GATC 1 cut(s) 168
BstMAI GTCTC 3 cut(s) 301, 411, 461
BstMBI GATC 1 cut(s) 165
BstMCI CGRYCG 2 cut(s) 338, 342
BstMWI GCNNNNNNNGC 3 cut(s) 376, 497, 506
BstNI CCWGG 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 280
BstSNI TACGTA 1 cut(s) 361
BstUI CGCG 1 cut(s) 159
BstV1I GCAGC 4 cut(s) 133, 450, 460, 493
BstV2I GAAGAC 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 335
BsuRI GGCC 1 cut(s) 337
BtgI CCRYGG 1 cut(s) 332
BtsCI GGATG 2 cut(s) 15, 270
BtsIMutI CAGTG 3 cut(s) 259, 288, 402
Cfr10I RCCGGY 1 cut(s) 337
CseI GACGC 1 cut(s) 148
Csp6I GTAC 1 cut(s) 358
CviAII CATG 2 cut(s) 122, 130
CviJI RGCY 8 cut(s) 19, 127, 228, 337, 404, 410, 463, 473
CviKI_1 RGCY 8 cut(s) 19, 127, 228, 337, 404, 410, 463, 473
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 1 cut(s) 245
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
EaeI YGGCCR 1 cut(s) 335
EagI CGGCCG 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Ecl136II GAGCTC 2 cut(s) 127, 404
EclXI CGGCCG 1 cut(s) 335
Eco105I TACGTA 1 cut(s) 361
Eco24I GRGCYC 2 cut(s) 129, 406
Eco31I GGTCTC 1 cut(s) 461
Eco52I CGGCCG 1 cut(s) 335
Eco53kI GAGCTC 2 cut(s) 127, 404
Eco88I CYCGRG 1 cut(s) 458
EcoICRI GAGCTC 2 cut(s) 127, 404
EcoRII CCWGG 1 cut(s) 280
EcoT38I GRGCYC 2 cut(s) 129, 406
FaeI CATG 2 cut(s) 125, 133
FaiI YATR 6 cut(s) 123, 131, 182, 195, 241, 324
FatI CATG 2 cut(s) 121, 129
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 4 cut(s) 147, 464, 474, 507
FokI GGATG 2 cut(s) 22, 277
FriOI GRGCYC 2 cut(s) 129, 406
Fsp4HI GCNGC 4 cut(s) 147, 464, 474, 507
FspBI CTAG 1 cut(s) 510
GluI GCNGC 4 cut(s) 147, 464, 474, 507
GsaI CCCAGC 1 cut(s) 234
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 1 cut(s) 337
HapII CCGG 1 cut(s) 338
HgaI GACGC 1 cut(s) 148
Hin1II CATG 2 cut(s) 125, 133
HpaII CCGG 1 cut(s) 338
HphI GGTGA 2 cut(s) 55, 185
Hpy166II GTNNAC 4 cut(s) 55, 76, 91, 481
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 2 cut(s) 163, 414
Hpy8I GTNNAC 4 cut(s) 55, 76, 91, 481
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 2 cut(s) 73, 397
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 1 cut(s) 491
HpyF10VI GCNNNNNNNGC 3 cut(s) 376, 497, 506
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 2 cut(s) 125, 133
Kzo9I GATC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 16, 409
Lsp1109I GCAGC 4 cut(s) 133, 450, 460, 493
MaeI CTAG 1 cut(s) 510
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 1 cut(s) 268
MalI GATC 1 cut(s) 167
MboI GATC 1 cut(s) 165
MboII GAAGA 5 cut(s) 45, 52, 188, 399, 525
MhlI GDGCHC 2 cut(s) 129, 406
MluCI AATT 2 cut(s) 327, 421
MnlI CCTC 5 cut(s) 40, 164, 165, 177, 377
MseI TTAA 1 cut(s) 200
MspI CCGG 1 cut(s) 338
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 348
MvaI CCWGG 1 cut(s) 282
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 376, 497, 506
NdeII GATC 1 cut(s) 165
NlaIII CATG 2 cut(s) 125, 133
NlaIV GGNNCC 2 cut(s) 18, 286
NmuCI GTSAC 1 cut(s) 268
PctI GAATGC 1 cut(s) 348
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 4 cut(s) 148, 465, 475, 508
Ppu21I YACGTR 1 cut(s) 361
Psp124BI GAGCTC 2 cut(s) 129, 406
Psp6I CCWGG 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 230
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 2 cut(s) 18, 286
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SacI GAGCTC 2 cut(s) 129, 406
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 4 cut(s) 147, 464, 474, 507
Sau3AI GATC 1 cut(s) 165
ScrFI CCNGG 1 cut(s) 282
SduI GDGCHC 2 cut(s) 129, 406
SnaBI TACGTA 1 cut(s) 361
Sse9I AATT 2 cut(s) 327, 421
SspI AATATT 1 cut(s) 438
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 2 cut(s) 129, 406
StyD4I CCNGG 1 cut(s) 280
TaaI ACNGT 2 cut(s) 73, 397
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 162, 318
TasI AATT 2 cut(s) 327, 421
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TscAI CASTG 3 cut(s) 266, 295, 402
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 4 cut(s) 146, 463, 473, 506
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 339
TspRI CASTG 3 cut(s) 266, 295, 402
Van91I CCANNNNNTGG 1 cut(s) 265
XapI RAATTY 1 cut(s) 327
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.