Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006213

Physical Location & Seq

Genomic Coordinates Forward (+)

29934776 .. 29938840

Gene Structure 4065 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1203600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2019 bp

ATGGGAACTTCAGTTGAGGTGACGCCGCTGTGCGGCGTATATAACGAGAATCCGCTGTCGTATATAGTTTGTAGGCAAAGGAGAGGGCATTGTGATTTCTCCCCATGTGTCTGGACATCTCTTGGGTGGTACCGTCTGGAAGATAACAAAGGATGGGGAGGATGTTATATATGCCTTGGACTTCAACCATCGAAAAGAGAAATTGATGATTTTGAACTGCTTACTGCTGTGATTTTCATCGGGCATTTGAATGGAATTAATCAATCAGCCTTCGTACGCCCAGCTGTTCTGATAACAGATGCCTACAATGCTTTGAACAATCAGCCTTATAGACGCCAAAAGGACAAGGAATTTACAGATACTATAAAGAAGACTCTGCGATCTGATGGGAATGTGCTGTTACCTGTTGATACTGCCGGGCGGGTTATGGAACTTGTCCAGATATTGGAATCGGTAGGGGAGATGAGTGCTGTTTTCTATCCTTGTTACAACAACAGAACAGTTTTCTCTTGTCATTGTTGGACAGAGGAAAATTTGAACTATCCCATCTTCTTCCTAACATACGTTGCATCAAGCACAATCGATTACGTAAAAAGTTTCCTTGAGTGGATGAGTGATGCTATAGCAAAGTCCTTTGAGAAGACACGTGAGAATGTTTTTAATTTGAAGCGAATCAGACTTTTAGTTAACAAGAGTGAACTTGACAATGCTCCTGATGGTCCCAAGGTTGTTTTAGCATCCATGGCCAGTTTAGAAGCTGGTTTCTCTCATGATATATTTGTTGAATGGGCAAATGATGCCAAGAATCTTGTGCTTTTTACAGAACGAGCCCAGTTTGGAAGTTTAGCTCGCATGCTTCAGGCAGATCCGTCTCCAAAAGCTGTTAAGGTTACCATATCCAAAAGGGTCCCTTTGGTTGGGGATGAACTAATTGCTTATGAAGAAGAGCAGAACAGAATCGGAAAGGAAGGAGCTCTAAAGGCTAGTTTGATAAAAGAGGAGGAATCAAAAGCTTTTCATGGGGCTGATGTCAACACGAGTGATCCAATGATCATTGATGCTAGCAATACACATCCTTTGCCGGATGCAGTAGGTCCACATGGTGGTGGATACCGTGACATGTTAATAGATGGATTTACTCCTCCTTCAACCAGTGTTGCCCCCATGTTCCCCTTCTATGAGAATAATTCTGAGTGGGATGACTTTGGTGAAGTTATAAATCCAGATGATTATGTCATAAAAGATGAAGACATGGACCATGGAACTATGCCTGCAAGTTTTTATTGTGAATCATCATATTCTTTGGTTGGTGGTGATGTGGATGGAAAACTTGACGAAGGCTCTGCTAGTTTGATACTCGACATGAGGCCTTCAAAAGTTGTATCGACTGAATTGACGGTTCAAGTGAAGTGTTCATTGATTTATATGGACTTTGAGGGCCGGTCCGATGCGAGATCCATCAAATCAATTCTTTCCCATATGGCTCCTCTAAAGCTTGTTTTGGTTCACGGAACAGCTGAGGCCACGGAACATTTGAAGCAACACTGCTTGAAACATGTTTGCCCGCATGTTTATGCTCCCCAAATTGAAGAAACAATTGATGTCACTTCTGACTTATGTGCTTATAAGGTGCAACTGTCTGAGAAGTTAATGAGTAACGTGCTCTTTAAAAAGCTCGGAGATTACGAAATAGCTTGGGTTGATTCTGGGGCAGGAAAGACAGAAAATGAAATGCTATCTTTACAACCCCTCTCAACCCCGCCTCCACCGCATGAATATGTCCTTGTTGGTGACTTAAAAATGGCTAATTTCAAGCAATTTCTTTCCGACAAGGGTGTTCAGGTTGAATTTGCTGGTGGGGCGCTGCGATGCGGTGAATATGTGACATTACGCAAAGTTGGGGATGCAAGCCATAAGGGTGGTGGTTCCGGAACCCAACAAATTGTGATCGAAGGCCCCTTATGCGAGGATTATTACAAGATTCGGGAGTATCTCTACTCACAATTTTATTTGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005847 GO:0005849 GO:0005911 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0030054 GO:0031047 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0055044 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Beta-Casp CPSF100_C Lactamase_B_6 RMMBL

Protein Analysis

673

Amino Acids

74.93

Weight (kDa)

5.05

Isoelectric Point (pI)

36.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-Casp	PF10996	174 - 286	2.5e-18	Beta-Casp domain
RMMBL	PF07521	467 - 517	2.3e-10	Zn-dependent metallo-hydrolase RNA specificity domain
CPSF100_C	PF13299	574 - 669	2.1e-17	Cleavage and polyadenylation factor 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0006213)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23880
fragaria_vesca	FvH4_5g14450 FvH4_7g29660 FvH4_7g29660
malus_domestica	MD01G1203600.v1.1 MD07G1272900.v1.1
prunus_persica	Prupe.2G296200_v2.0.a1 Prupe.2G296200_v2.0.a1
pyrus_communis	pycom01g21270 pycom01g21280 pycom07g24930
rosa_chinensis	RchiOBHm_Chr1g0377671 RchiOBHm_Chr1g0377681 RchiOBHm_Chr1g0379161 RchiOBHm_Chr1g0379331
rosa_laevigata	RLG00000026506 RLG00000026773
rosa_multiflora	Rmu_sc0002832.1_g000015
rosa_roxburghii	Rroxscaffold_4G00281130
rosa_rugosa	Rorug01G0404200 Rorug01G0404300
rosa_samantha	Rh1AG428300 Rh1BG381000 Rh1CG000300 Rh1CG395000 Rh1DG412000
rosa_wichuraiana	Rw1G037040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1217, 1626
Acc65I	GGTACC	1 cut(s)	129
AccB1I	GGYRCC	1 cut(s)	129
AccB7I	CCANNNNNTGG	2 cut(s)	445, 1103
AccIII	TCCGGA	1 cut(s)	1928
AciI	CCGC	8 cut(s)	26, 33, 53, 421, 1565, 1760, 1769, 1872
AclWI	GGATC	3 cut(s)	860, 1037, 1449
AcoI	YGGCCR	1 cut(s)	744
AcsI	RAATTY	3 cut(s)	350, 532, 1847
AcuI	CTGAAG	1 cut(s)	842
AcvI	CACGTG	1 cut(s)	647
AcyI	GRCGYC	2 cut(s)	23, 334
AdeI	CACNNNGTG	1 cut(s)	1103
AfaI	GTAC	2 cut(s)	131, 276
AfiI	CCNNNNNNNGG	4 cut(s)	32, 445, 917, 1103
AflIII	ACRYGT	3 cut(s)	644, 1119, 1555
Alw21I	GWGCWC	2 cut(s)	976, 1665
Alw26I	GTCTC	1 cut(s)	876
AlwI	GGATC	3 cut(s)	860, 1037, 1449
Aor13HI	TCCGGA	1 cut(s)	1928
AoxI	GGCC	5 cut(s)	744, 1367, 1438, 1521, 1954
ApeKI	GCWGC	1 cut(s)	1864
ApoI	RAATTY	3 cut(s)	350, 532, 1847
AseI	ATTAAT	1 cut(s)	258
Asp718I	GGTACC	1 cut(s)	129
AspLEI	GCGC	1 cut(s)	1864
AspS9I	GGNCC	7 cut(s)	719, 907, 1094, 1255, 1438, 1443, 1955
AsuC2I	CCSGG	1 cut(s)	418
AsuHPI	GGTGA	5 cut(s)	31, 1220, 1325, 1802, 1886
AsuNHI	GCTAGC	1 cut(s)	1061
AvaII	GGWCC	5 cut(s)	719, 907, 1094, 1255, 1443
BalI	TGGCCA	1 cut(s)	746
BanI	GGYRCC	1 cut(s)	129
BanII	GRGCYC	2 cut(s)	832, 976
BauI	CACGAG	1 cut(s)	1036
BbrPI	CACGTG	1 cut(s)	647
BbsI	GAAGAC	3 cut(s)	377, 647, 1254
Bbv12I	GWGCWC	2 cut(s)	976, 1665
BbvCI	CCTCAGC	1 cut(s)	1518
BbvI	GCAGC	1 cut(s)	1851
BccI	CCATC	8 cut(s)	147, 196, 380, 554, 710, 1124, 1316, 1466
BcgI	CGANNNNNNTGC	2 cut(s)	1325, 1359
BciVI	GTATCC	1 cut(s)	1103
BclI	TGATCA	1 cut(s)	1050
BcnI	CCSGG	1 cut(s)	418
BcoDI	GTCTC	1 cut(s)	876
BfaI	CTAG	3 cut(s)	984, 1062, 1347
BfmI	CTRYAG	1 cut(s)	621
BfoI	RGCGCY	1 cut(s)	1865
BfuI	GTATCC	1 cut(s)	1103
BisI	GCNGC	3 cut(s)	26, 34, 1865
BlsI	GCNGC	3 cut(s)	27, 35, 1866
Bme1390I	CCNGG	1 cut(s)	418
Bme18I	GGWCC	5 cut(s)	719, 907, 1094, 1255, 1443
BmgT120I	GGNCC	7 cut(s)	719, 907, 1094, 1255, 1438, 1443, 1955
BmiI	GGNNCC	8 cut(s)	131, 721, 908, 909, 1485, 1927, 1933, 1957
BmrFI	CCNGG	1 cut(s)	418
BmrI	ACTGGG	1 cut(s)	826
BmtI	GCTAGC	1 cut(s)	1065
BmuI	ACTGGG	1 cut(s)	826
BpiI	GAAGAC	3 cut(s)	377, 647, 1254
Bpu10I	CCTNAGC	1 cut(s)	1518
BpuEI	CTTGAG	1 cut(s)	623
BpuMI	CCSGG	1 cut(s)	418
Bsa29I	ATCGAT	1 cut(s)	582
BsaAI	YACGTR	2 cut(s)	589, 647
BsaBI	GATNNNNATC	1 cut(s)	236
BsaHI	GRCGYC	2 cut(s)	23, 334
BsaJI	CCNNGG	5 cut(s)	175, 723, 741, 1258, 1524
BsaWI	WCCGGW	1 cut(s)	1928
BsaXI	ACNNNNNCTCC	2 cut(s)	1747, 1777
Bsc4I	CCNNNNNNNGG	4 cut(s)	32, 445, 917, 1103
Bse118I	RCCGGY	1 cut(s)	1440
Bse1I	ACTGG	3 cut(s)	747, 832, 1152
Bse8I	GATNNNNATC	1 cut(s)	236
BseAI	TCCGGA	1 cut(s)	1928
BseCI	ATCGAT	1 cut(s)	582
BseDI	CCNNGG	5 cut(s)	175, 723, 741, 1258, 1524
BseJI	GATNNNNATC	1 cut(s)	236
BseLI	CCNNNNNNNGG	4 cut(s)	32, 445, 917, 1103
BseMII	CTCAG	3 cut(s)	1182, 1509, 1632
BseNI	ACTGG	3 cut(s)	747, 832, 1152
BseRI	GAGGAG	3 cut(s)	1013, 1131, 1476
BseXI	GCAGC	1 cut(s)	1851
BseYI	CCCAGC	1 cut(s)	280
BshFI	GGCC	5 cut(s)	746, 1369, 1440, 1523, 1956
BshNI	GGYRCC	1 cut(s)	129
BshVI	ATCGAT	1 cut(s)	582
BsiHKAI	GWGCWC	2 cut(s)	976, 1665
BsiSI	CCGG	4 cut(s)	417, 1082, 1441, 1929
BsiWI	CGTACG	1 cut(s)	274
BslFI	GGGAC	2 cut(s)	705, 893
BslI	CCNNNNNNNGG	4 cut(s)	32, 445, 917, 1103
BsmAI	GTCTC	1 cut(s)	876
BsmBI	CGTCTC	1 cut(s)	876
BsmFI	GGGAC	2 cut(s)	705, 893
BsnI	GGCC	5 cut(s)	746, 1369, 1440, 1523, 1956
Bsp1286I	GDGCHC	3 cut(s)	832, 976, 1665
Bsp13I	TCCGGA	1 cut(s)	1928
Bsp143I	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
Bsp19I	CCATGG	2 cut(s)	741, 1258
BspACI	CCGC	8 cut(s)	26, 33, 53, 421, 1565, 1760, 1769, 1872
BspANI	GGCC	5 cut(s)	746, 1369, 1440, 1523, 1956
BspCNI	CTCAG	3 cut(s)	1183, 1510, 1633
BspDI	ATCGAT	1 cut(s)	582
BspEI	TCCGGA	1 cut(s)	1928
BspHI	TCATGA	1 cut(s)	769
BspLI	GGNNCC	8 cut(s)	131, 721, 908, 909, 1485, 1927, 1933, 1957
BspOI	GCTAGC	1 cut(s)	1065
BspPI	GGATC	3 cut(s)	860, 1037, 1449
BspQI	GCTCTTC	1 cut(s)	939
BspT107I	GGYRCC	1 cut(s)	129
BsrFI	RCCGGY	1 cut(s)	1440
BsrI	ACTGG	3 cut(s)	747, 832, 1152
BssAI	RCCGGY	1 cut(s)	1440
BssECI	CCNNGG	5 cut(s)	175, 723, 741, 1258, 1524
BssMI	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
BssNI	GRCGYC	2 cut(s)	23, 334
BssSI	CACGAG	1 cut(s)	1036
BssT1I	CCWWGG	4 cut(s)	175, 723, 741, 1258
Bst2BI	CACGAG	1 cut(s)	1036
Bst4CI	ACNGT	5 cut(s)	134, 502, 1115, 1399, 1638
Bst6I	CTCTTC	1 cut(s)	939
BstACI	GRCGYC	2 cut(s)	23, 334
BstAPI	GCANNNNNTGC	1 cut(s)	797
BstBAI	YACGTR	2 cut(s)	589, 647
BstC8I	GCNNGC	6 cut(s)	850, 854, 1063, 1272, 1565, 1909
BstDEI	CTNAG	3 cut(s)	1191, 1518, 1641
BstDSI	CCRYGG	3 cut(s)	741, 1258, 1524
BstEII	GGTNACC	1 cut(s)	889
BstH2I	RGCGCY	1 cut(s)	1865
BstHHI	GCGC	1 cut(s)	1864
BstKTI	GATC	6 cut(s)	383, 868, 1045, 1053, 1457, 1950
BstMAI	GTCTC	1 cut(s)	876
BstMBI	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
BstMWI	GCNNNNNNNGC	7 cut(s)	308, 743, 797, 980, 1768, 1859, 1962
BstNSI	RCATGY	4 cut(s)	856, 1123, 1559, 1571
BstPI	GGTNACC	1 cut(s)	889
BstSCI	CCNGG	1 cut(s)	416
BstSFI	CTRYAG	1 cut(s)	621
BstSNI	TACGTA	1 cut(s)	589
BstV1I	GCAGC	1 cut(s)	1851
BstV2I	GAAGAC	3 cut(s)	377, 647, 1254
BstX2I	RGATCY	2 cut(s)	865, 1454
BstXI	CCANNNNNNTGG	2 cut(s)	111, 1919
BstYI	RGATCY	2 cut(s)	865, 1454
Bsu15I	ATCGAT	1 cut(s)	582
BsuI	GTATCC	1 cut(s)	1103
BsuRI	GGCC	5 cut(s)	746, 1369, 1440, 1523, 1956
BsuTUI	ATCGAT	1 cut(s)	582
BtgI	CCRYGG	3 cut(s)	741, 1258, 1524
BtgZI	GCGATG	1 cut(s)	1882
BtsI	GCAGTG	1 cut(s)	1543
BtsIMutI	CAGTG	2 cut(s)	1159, 1543
Cac8I	GCNNGC	6 cut(s)	850, 854, 1063, 1272, 1565, 1909
CciI	TCATGA	1 cut(s)	769
CfoI	GCGC	1 cut(s)	1864
Cfr10I	RCCGGY	1 cut(s)	1440
Cfr13I	GGNCC	7 cut(s)	719, 907, 1094, 1255, 1438, 1443, 1955
ClaI	ATCGAT	1 cut(s)	582
CpoI	CGGWCCG	1 cut(s)	1443
CseI	GACGC	2 cut(s)	31, 342
Csp6I	GTAC	2 cut(s)	130, 275
CspI	CGGWCCG	1 cut(s)	1443
CviQI	GTAC	2 cut(s)	130, 275
DdeI	CTNAG	3 cut(s)	1191, 1518, 1641
DpnI	GATC	6 cut(s)	382, 867, 1044, 1052, 1456, 1949
DpnII	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
DraI	TTTAAA	1 cut(s)	1669
DraIII	CACNNNGTG	1 cut(s)	1103
EaeI	YGGCCR	1 cut(s)	744
Eam1104I	CTCTTC	1 cut(s)	939
EarI	CTCTTC	1 cut(s)	939
Ecl136II	GAGCTC	1 cut(s)	974
Eco105I	TACGTA	1 cut(s)	589
Eco130I	CCWWGG	4 cut(s)	175, 723, 741, 1258
Eco147I	AGGCCT	1 cut(s)	1369
Eco24I	GRGCYC	2 cut(s)	832, 976
Eco47I	GGWCC	5 cut(s)	719, 907, 1094, 1255, 1443
Eco53kI	GAGCTC	1 cut(s)	974
Eco57I	CTGAAG	1 cut(s)	842
Eco72I	CACGTG	1 cut(s)	647
Eco91I	GGTNACC	1 cut(s)	889
EcoICRI	GAGCTC	1 cut(s)	974
EcoO109I	RGGNCCY	2 cut(s)	907, 1955
EcoO65I	GGTNACC	1 cut(s)	889
EcoT14I	CCWWGG	4 cut(s)	175, 723, 741, 1258
EcoT38I	GRGCYC	2 cut(s)	832, 976
ErhI	CCWWGG	4 cut(s)	175, 723, 741, 1258
Esp3I	CGTCTC	1 cut(s)	876
FalI	AAGNNNNNCTT	2 cut(s)	895, 927
FaqI	GGGAC	2 cut(s)	705, 893
FauI	CCCGC	3 cut(s)	414, 1572, 1767
FauNDI	CATATG	1 cut(s)	1479
FbaI	TGATCA	1 cut(s)	1050
Fnu4HI	GCNGC	3 cut(s)	26, 34, 1865
FriOI	GRGCYC	2 cut(s)	832, 976
Fsp4HI	GCNGC	3 cut(s)	26, 34, 1865
FspBI	CTAG	3 cut(s)	984, 1062, 1347
GlaI	GCGC	1 cut(s)	1863
GluI	GCNGC	3 cut(s)	26, 34, 1865
GsaI	CCCAGC	1 cut(s)	284
HaeII	RGCGCY	1 cut(s)	1865
HaeIII	GGCC	5 cut(s)	746, 1369, 1440, 1523, 1956
HapII	CCGG	4 cut(s)	417, 1082, 1441, 1929
HgaI	GACGC	2 cut(s)	31, 342
HhaI	GCGC	1 cut(s)	1864
Hin1I	GRCGYC	2 cut(s)	23, 334
Hin6I	GCGC	1 cut(s)	1862
HinP1I	GCGC	1 cut(s)	1862
HincII	GTYRAC	2 cut(s)	688, 1033
HindII	GTYRAC	2 cut(s)	688, 1033
HindIII	AAGCTT	2 cut(s)	1011, 1493
HpaI	GTTAAC	1 cut(s)	688
HpaII	CCGG	4 cut(s)	417, 1082, 1441, 1929
HphI	GGTGA	5 cut(s)	31, 1220, 1325, 1802, 1886
Hpy166II	GTNNAC	5 cut(s)	688, 698, 1033, 1097, 1507
Hpy188III	TCNNGA	8 cut(s)	112, 137, 439, 713, 770, 1223, 1929, 1985
Hpy8I	GTNNAC	5 cut(s)	688, 698, 1033, 1097, 1507
HpyAV	CCTTC	7 cut(s)	280, 962, 1155, 1183, 1331, 1380, 1946
HpyCH4III	ACNGT	5 cut(s)	134, 502, 1115, 1399, 1638
HpyCH4IV	ACGT	4 cut(s)	564, 588, 646, 1659
HpyCH4V	TGCA	5 cut(s)	569, 1088, 1274, 1633, 1907
HpyF10VI	GCNNNNNNNGC	7 cut(s)	308, 743, 797, 980, 1768, 1859, 1962
HpyF3I	CTNAG	3 cut(s)	1191, 1518, 1641
HpySE526I	ACGT	4 cut(s)	564, 588, 646, 1659
Hsp92I	GRCGYC	2 cut(s)	23, 334
HspAI	GCGC	1 cut(s)	1862
KflI	GGGWCCC	1 cut(s)	907
Kpn2I	TCCGGA	1 cut(s)	1928
KpnI	GGTACC	1 cut(s)	133
Ksp22I	TGATCA	1 cut(s)	1050
KspAI	GTTAAC	1 cut(s)	688
Kzo9I	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
LguI	GCTCTTC	1 cut(s)	939
LmnI	GCTCC	4 cut(s)	715, 971, 1489, 1582
Lsp1109I	GCAGC	1 cut(s)	1851
MaeI	CTAG	3 cut(s)	984, 1062, 1347
MaeII	ACGT	4 cut(s)	564, 588, 646, 1659
MaeIII	GTNAC	9 cut(s)	19, 399, 485, 889, 1115, 1603, 1655, 1790, 1882
MalI	GATC	6 cut(s)	382, 867, 1044, 1052, 1456, 1949
MboI	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
MboII	GAAGA	9 cut(s)	152, 382, 541, 544, 652, 953, 956, 1259, 1601
MfeI	CAATTG	1 cut(s)	1596
MflI	RGATCY	2 cut(s)	865, 1454
MhlI	GDGCHC	3 cut(s)	832, 976, 1665
MlsI	TGGCCA	1 cut(s)	746
MluNI	TGGCCA	1 cut(s)	746
MlyI	GAGTC	1 cut(s)	367
MmeI	TCCRAC	2 cut(s)	500, 1851
Mox20I	TGGCCA	1 cut(s)	746
MroI	TCCGGA	1 cut(s)	1928
MscI	TGGCCA	1 cut(s)	746
MseI	TTAA	9 cut(s)	258, 660, 687, 885, 1124, 1649, 1668, 1796, 2017
MslI	CAYNNNNRTG	5 cut(s)	249, 1104, 1572, 1776, 1917
Msp20I	TGGCCA	1 cut(s)	746
MspA1I	CMGCKG	4 cut(s)	28, 55, 284, 1517
MspI	CCGG	4 cut(s)	417, 1082, 1441, 1929
MspR9I	CCNGG	1 cut(s)	418
MunI	CAATTG	1 cut(s)	1596
MwoI	GCNNNNNNNGC	7 cut(s)	308, 743, 797, 980, 1768, 1859, 1962
NciI	CCSGG	1 cut(s)	418
NcoI	CCATGG	2 cut(s)	741, 1258
NdeI	CATATG	1 cut(s)	1479
NdeII	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
NheI	GCTAGC	1 cut(s)	1061
NlaIV	GGNNCC	8 cut(s)	131, 721, 908, 909, 1485, 1927, 1933, 1957
NmuCI	GTSAC	5 cut(s)	19, 1115, 1603, 1790, 1882
NspI	RCATGY	4 cut(s)	856, 1123, 1559, 1571
PaeI	GCATGC	1 cut(s)	856
PagI	TCATGA	1 cut(s)	769
PceI	AGGCCT	1 cut(s)	1369
PciI	ACATGT	2 cut(s)	1119, 1555
PciSI	GCTCTTC	1 cut(s)	939
PcsI	WCGNNNNNNNCGW	1 cut(s)	1683
PfeI	GAWTC	9 cut(s)	49, 449, 672, 805, 957, 1004, 1289, 1703, 1981
Pfl23II	CGTACG	1 cut(s)	274
PflMI	CCANNNNNTGG	2 cut(s)	445, 1103
PkrI	GCNGC	3 cut(s)	27, 35, 1866
PleI	GAGTC	1 cut(s)	367
PmaCI	CACGTG	1 cut(s)	647
PmlI	CACGTG	1 cut(s)	647
PpsI	GAGTC	1 cut(s)	367
Ppu21I	YACGTR	2 cut(s)	589, 647
PpuMI	RGGWCCY	1 cut(s)	907
PscI	ACATGT	2 cut(s)	1119, 1555
PshBI	ATTAAT	1 cut(s)	258
PsiI	TTATAA	2 cut(s)	1217, 1626
Psp124BI	GAGCTC	1 cut(s)	976
Psp5II	RGGWCCY	1 cut(s)	907
PspCI	CACGTG	1 cut(s)	647
PspEI	GGTNACC	1 cut(s)	889
PspFI	CCCAGC	1 cut(s)	280
PspLI	CGTACG	1 cut(s)	274
PspN4I	GGNNCC	8 cut(s)	131, 721, 908, 909, 1485, 1927, 1933, 1957
PspPI	GGNCC	7 cut(s)	719, 907, 1094, 1255, 1438, 1443, 1955
PspPPI	RGGWCCY	1 cut(s)	907
PsuI	RGATCY	2 cut(s)	865, 1454
PvuII	CAGCTG	2 cut(s)	284, 1517
RsaI	GTAC	2 cut(s)	131, 276
RsaNI	GTAC	2 cut(s)	130, 275
RseI	CAYNNNNRTG	5 cut(s)	249, 1104, 1572, 1776, 1917
Rsr2I	CGGWCCG	1 cut(s)	1443
RsrII	CGGWCCG	1 cut(s)	1443
SacI	GAGCTC	1 cut(s)	976
SapI	GCTCTTC	1 cut(s)	939
SaqAI	TTAA	9 cut(s)	258, 660, 687, 885, 1124, 1649, 1668, 1796, 2017
SatI	GCNGC	3 cut(s)	26, 34, 1865
Sau3AI	GATC	6 cut(s)	380, 865, 1042, 1050, 1454, 1947
Sau96I	GGNCC	7 cut(s)	719, 907, 1094, 1255, 1438, 1443, 1955
SchI	GAGTC	1 cut(s)	367
ScrFI	CCNGG	1 cut(s)	418
SduI	GDGCHC	3 cut(s)	832, 976, 1665
SfcI	CTRYAG	1 cut(s)	621
SinI	GGWCC	5 cut(s)	719, 907, 1094, 1255, 1443
SmiMI	CAYNNNNRTG	5 cut(s)	249, 1104, 1572, 1776, 1917
SmlI	CTYRAG	1 cut(s)	602
SmoI	CTYRAG	1 cut(s)	602
SnaBI	TACGTA	1 cut(s)	589
SphI	GCATGC	1 cut(s)	856
SseBI	AGGCCT	1 cut(s)	1369
SsiI	CCGC	8 cut(s)	26, 33, 53, 421, 1565, 1760, 1769, 1872
SspMI	CTAG	3 cut(s)	984, 1062, 1347
SstI	GAGCTC	1 cut(s)	976
StuI	AGGCCT	1 cut(s)	1369
StyD4I	CCNGG	1 cut(s)	416
StyI	CCWWGG	4 cut(s)	175, 723, 741, 1258
TaaI	ACNGT	5 cut(s)	134, 502, 1115, 1399, 1638
TaiI	ACGT	4 cut(s)	567, 591, 649, 1662
TaqI	TCGA	5 cut(s)	191, 582, 1359, 1385, 1950
TauI	GCSGC	2 cut(s)	28, 36
TfiI	GAWTC	9 cut(s)	49, 449, 672, 805, 957, 1004, 1289, 1703, 1981
Tru1I	TTAA	9 cut(s)	258, 660, 687, 885, 1124, 1649, 1668, 1796, 2017
Tru9I	TTAA	9 cut(s)	258, 660, 687, 885, 1124, 1649, 1668, 1796, 2017
TscAI	CASTG	2 cut(s)	1159, 1550
TseFI	GTSAC	5 cut(s)	19, 1115, 1603, 1790, 1882
TseI	GCWGC	1 cut(s)	1864
Tsp45I	GTSAC	5 cut(s)	19, 1115, 1603, 1790, 1882
TspDTI	ATGAA	8 cut(s)	226, 939, 954, 1007, 1260, 1404, 1743, 1788
TspGWI	ACGGA	3 cut(s)	858, 1524, 1541
TspRI	CASTG	2 cut(s)	1159, 1550
Van91I	CCANNNNNTGG	2 cut(s)	445, 1103
VpaK11BI	GGWCC	5 cut(s)	719, 907, 1094, 1255, 1443
VspI	ATTAAT	1 cut(s)	258
XapI	RAATTY	3 cut(s)	350, 532, 1847
XceI	RCATGY	4 cut(s)	856, 1123, 1559, 1571
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1919
XspI	CTAG	3 cut(s)	984, 1062, 1347

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.