Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0006213

Physical Location & Seq

Genomic Coordinates Forward (+)

33907543 .. 33912430

Gene Structure 4888 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1272900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGGAACTTCAGTTCAGGTAACGCCGTTGTGCGGCGTCTACAACGAGAACCCGCTGTCGTATTTGGTCTCAATCGACGGCTTCAACCTCCTCATCGACTGCGGCTGGAACGACCACTTCGACCCCTCCCTCCTTCAACCCCTCTCCAGGGTAGCTTCGACGGTGGATGCAGTGTTGTTGTCGCATCCAGATACACTTCACCTCGGCGCGCTTCCGTACGCCATGAAGCAGCTCGGCCTCTCTGCGCCCGTCTTCTCCACCGAGCCGGTTTACAGATTAGGTCTCCTCACTATGTATGATCAGTTCCTCTCTCGCAAGCAAGTATCAGACTTTGACCTTTTTACACTGGATGACATTGATTCTGCATTCCAAAATTTCACCAGATTAACCTATGCTCAAAATCACCATCTTTCTGGCAAAGGAGAGGGAATTGTGATTTCACCCCATGTGTCTGGGCATCTCTTGGGCGGTACCGTCTGGAAGATAACAAAGGATGGGGAGGATGTTATATATGCTGTGGACTTCAACCATCGAAAAGAGAAGCATTTGAACGGAATTAATCAATCAGCCTTCGTACGCCCTGCTGTTCTGATAACAGATGCCTATAATGCTTTGAACAATCAGCCTTATAGACGCCAAAAGGACAAGGAATTTACAGATGCTATAAAGAAGACTCTACGATCTGATGGAAATGTGCTATTACCTGTTGATACTGCCGGCCGGGTTATGGAACTTGTCCAGATATTGGAATCGTGTTGGACAGAGGAAAATTTGAACTATCCCATCTTCTTTCTAACATACGTTGCGTCTAGCACAATCGATTACGTAAAAAGTTTCCTTGAGTGGATGAGTGATGCGATAGCAAAGTCCTTTGAGAAAACACGTGAGAATGTTTTTAATTTGAAGCGAATCAGACTTCTAGTTAGCAAGAGTGAACTTGACGATGCTCCTGACGGTCCCAAGGTTGTTTTAGCATCCATGGCCAGTTTAGAAGCTGGTTTCTCTCACGATATATTTGTAGAATGGGCAAATGACCCCAAGAATCTTGTCCTTTTTACAGAACGAGCCCAGTTTGGAAGTTTAGCTCGCATGCTTCAGGCAGATCCGCCTCCAAAAGCTGTTAAGGTTACCATATCCAAAAGGGTCCCTTTGGTTGGTGAGGAGCTAATTGCTTATGAAGAAGAGCAGAACAGAATAAGAAAGGAAGAAGCTCTAAAGGCTAGTCTTGTAAAAGAGGAGGAATCAAAAGCTTCTCATGGGGCTGATGTCAACACGAGTGATCCAATGATCATTGATGCTAGTAATACACATTCTTTGCCGGATGCAGCAGGTCCACAAGGCAGTGGATACCGGGACATTTTAATAGATGGATTTACTCCTCCTTCTACCAGTGTTGCCCCTATGTTTCCCTTCTATGAGAATAGTACCGAGTGGGATGACTTTGGTGAAGTTATAAATCCGGATGATTATGTCATAAAGGATGAAGACATGGACCATGGAGCTATGCATGTTGGTGGTGATATGGATGGAAAACTTGATGAAGGCTCTGCTAGTTTGATACTTGACACGAGGCCTTCAAAAGTTGTATCGACTGAATTGACCGTTCAAGTGAAGTGTTCATTGATTTATATGGACTTTGAGGGCCGTTCCGATGCAAGATCCATCAAATCAATTCTTTCCCATATGGCTCCTCTAAAACTTGTTTTGGTTCATGGAACAGCTGAGGCCACGGAACATTTGAAGCAACACTGCTTGAAACACGTTTGTCCGCATGTTTATGCTCCTCAAATTGAAGAAACAATTGATGTTACTTCTGATTTATGCGCTTATAAGGTGCAATTGTCTGAGAAGTTAATGAGTAACGTGCTCTTTAAAAAGGTTGGAGATTACGAAATAGCATGGGTTGACTCTGAGGCAGGAAAGACAGAAAATGATATGCTATCTTTACAACCCCTCTCAAACCCACCTCCACCACATGAATCTGTCCTTGTTGGTGACTTAAAAATGGCTAATTTCAAGCAATTTCTTTCCGACAAAGGTGTTCAGGCTGAATTTGCTGGTGGCGTGCTGAGATGCGGTGAATATGTGACACTACGCAAAGTTGGGGATGCAAGCCATAAGGGTGGTGGTTCCGGCACCCAACAAATTGTGATCGAAGGCCCCTTATGTGAGGATTATTACAAGATTCGGGAGTATCTCTACTCACAATTTTATTTGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005847 GO:0005849 GO:0005911 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0030054 GO:0031047 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0055044 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Beta-Casp CPSF100_C Lactamase_B_6 RMMBL

Protein Analysis

741

Amino Acids

82.21

Weight (kDa)

5.03

Isoelectric Point (pI)

34.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	19 - 146	1.4e-06	Metallo-beta-lactamase superfamily
Lactamase_B_6	PF16661	22 - 192	1.8e-54	Metallo-beta-lactamase superfamily domain
Beta-Casp	PF10996	243 - 365	2.1e-21	Beta-Casp domain
RMMBL	PF07521	535 - 585	2.6e-10	Zn-dependent metallo-hydrolase RNA specificity domain
CPSF100_C	PF13299	642 - 737	5.2e-19	Cleavage and polyadenylation factor 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006213)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23880
fragaria_vesca	FvH4_5g14450 FvH4_7g29660 FvH4_7g29660
malus_domestica	MD01G1203600.v1.1 MD07G1272900.v1.1
prunus_persica	Prupe.2G296200_v2.0.a1 Prupe.2G296200_v2.0.a1
pyrus_communis	pycom01g21270 pycom01g21280 pycom07g24930
rosa_chinensis	RchiOBHm_Chr1g0377671 RchiOBHm_Chr1g0377681 RchiOBHm_Chr1g0379161 RchiOBHm_Chr1g0379331
rosa_laevigata	RLG00000026506 RLG00000026773
rosa_multiflora	Rmu_sc0002832.1_g000015
rosa_roxburghii	Rroxscaffold_4G00281130
rosa_rugosa	Rorug01G0404200 Rorug01G0404300
rosa_samantha	Rh1AG428300 Rh1BG381000 Rh1CG000300 Rh1CG395000 Rh1DG412000
rosa_wichuraiana	Rw1G037040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1454, 1830
Acc36I	ACCTGC	1 cut(s)	1319
Acc65I	GGTACC	1 cut(s)	470
AccB1I	GGYRCC	2 cut(s)	470, 2135
AccB7I	CCANNNNNTGG	1 cut(s)	745
AccI	GTMKAC	1 cut(s)	39
AccII	CGCG	1 cut(s)	209
AccIII	TCCGGA	1 cut(s)	1459
AciI	CCGC	7 cut(s)	33, 53, 102, 468, 1106, 1769, 2076
AclWI	GGATC	3 cut(s)	1097, 1274, 1653
AcoI	YGGCCR	2 cut(s)	718, 981
AcsI	RAATTY	4 cut(s)	373, 650, 769, 2051
AcuI	CTGAAG	1 cut(s)	1079
AcvI	CACGTG	1 cut(s)	884
AcyI	GRCGYC	2 cut(s)	36, 634
AfaI	GTAC	4 cut(s)	218, 472, 576, 1426
AfiI	CCNNNNNNNGG	6 cut(s)	32, 147, 148, 727, 745, 1154
AflIII	ACRYGT	2 cut(s)	881, 1759
AjnI	CCWGG	1 cut(s)	146
AloI	GAACNNNNNNTCC	1 cut(s)	29
Alw21I	GWGCWC	1 cut(s)	1869
Alw26I	GTCTC	2 cut(s)	73, 287
AlwI	GGATC	3 cut(s)	1097, 1274, 1653
Aor13HI	TCCGGA	1 cut(s)	1459
AoxI	GGCC	7 cut(s)	235, 718, 981, 1571, 1642, 1725, 2158
ApeKI	GCWGC	2 cut(s)	229, 1325
ApoI	RAATTY	4 cut(s)	373, 650, 769, 2051
AseI	ATTAAT	1 cut(s)	558
Asp718I	GGTACC	1 cut(s)	470
AspLEI	GCGC	4 cut(s)	209, 211, 247, 1826
AspS9I	GGNCC	6 cut(s)	956, 1144, 1331, 1492, 1642, 2159
AsuC2I	CCSGG	2 cut(s)	722, 1352
AsuHPI	GGTGA	9 cut(s)	191, 370, 395, 432, 1169, 1457, 1529, 2006, 2090
AvaII	GGWCC	4 cut(s)	956, 1144, 1331, 1492
BalI	TGGCCA	1 cut(s)	983
BanI	GGYRCC	2 cut(s)	470, 2135
BanII	GRGCYC	1 cut(s)	1069
BauI	CACGAG	2 cut(s)	1273, 1567
BbrPI	CACGTG	1 cut(s)	884
BbsI	GAAGAC	3 cut(s)	244, 677, 1491
Bbv12I	GWGCWC	1 cut(s)	1869
BbvCI	CCTCAGC	1 cut(s)	1722
BbvI	GCAGC	2 cut(s)	241, 1337
BccI	CCATC	8 cut(s)	414, 488, 537, 680, 791, 1361, 1520, 1670
BceAI	ACGGC	3 cut(s)	10, 94, 1629
BciT130I	CCWGG	1 cut(s)	148
BciVI	GTATCC	1 cut(s)	1340
BclI	TGATCA	2 cut(s)	298, 1287
BcnI	CCSGG	2 cut(s)	722, 1352
BcoDI	GTCTC	2 cut(s)	73, 287
BfaI	CTAG	5 cut(s)	810, 920, 1221, 1299, 1551
BfuAI	ACCTGC	1 cut(s)	1319
BfuI	GTATCC	1 cut(s)	1340
BisI	GCNGC	4 cut(s)	34, 103, 230, 1326
BlsI	GCNGC	4 cut(s)	35, 104, 231, 1327
Bme1390I	CCNGG	3 cut(s)	148, 722, 1352
Bme18I	GGWCC	4 cut(s)	956, 1144, 1331, 1492
BmgT120I	GGNCC	6 cut(s)	956, 1144, 1331, 1492, 1642, 2159
BmiI	GGNNCC	8 cut(s)	472, 958, 1145, 1146, 1689, 2131, 2137, 2161
BmrFI	CCNGG	3 cut(s)	148, 722, 1352
BmrI	ACTGGG	1 cut(s)	1063
BmuI	ACTGGG	1 cut(s)	1063
BpiI	GAAGAC	3 cut(s)	244, 677, 1491
BpmI	CTGGAG	1 cut(s)	130
Bpu10I	CCTNAGC	1 cut(s)	1722
BpuEI	CTTGAG	1 cut(s)	860
BpuMI	CCSGG	2 cut(s)	722, 1352
Bsa29I	ATCGAT	1 cut(s)	819
BsaAI	YACGTR	2 cut(s)	826, 884
BsaHI	GRCGYC	2 cut(s)	36, 634
BsaI	GGTCTC	2 cut(s)	73, 287
BsaJI	CCNNGG	6 cut(s)	147, 202, 960, 978, 1495, 1728
BsaWI	WCCGGW	1 cut(s)	1459
Bsc4I	CCNNNNNNNGG	6 cut(s)	32, 147, 148, 727, 745, 1154
Bse118I	RCCGGY	2 cut(s)	265, 716
Bse1I	ACTGG	4 cut(s)	351, 984, 1069, 1389
BseAI	TCCGGA	1 cut(s)	1459
BseBI	CCWGG	1 cut(s)	148
BseCI	ATCGAT	1 cut(s)	819
BseDI	CCNNGG	6 cut(s)	147, 202, 960, 978, 1495, 1728
BseLI	CCNNNNNNNGG	6 cut(s)	32, 147, 148, 727, 745, 1154
BseMII	CTCAG	4 cut(s)	1713, 1836, 1902, 2060
BseNI	ACTGG	4 cut(s)	351, 984, 1069, 1389
BsePI	GCGCGC	1 cut(s)	207
BseRI	GAGGAG	7 cut(s)	80, 275, 1175, 1250, 1368, 1680, 1773
BseX3I	CGGCCG	1 cut(s)	718
BseXI	GCAGC	2 cut(s)	241, 1337
Bsh1236I	CGCG	1 cut(s)	209
Bsh1285I	CGRYCG	1 cut(s)	721
BshFI	GGCC	7 cut(s)	237, 720, 983, 1573, 1644, 1727, 2160
BshNI	GGYRCC	2 cut(s)	470, 2135
BshVI	ATCGAT	1 cut(s)	819
BsiEI	CGRYCG	1 cut(s)	721
BsiHKAI	GWGCWC	1 cut(s)	1869
BsiSI	CCGG	7 cut(s)	266, 717, 721, 1319, 1351, 1460, 2133
BsiWI	CGTACG	2 cut(s)	216, 574
BslFI	GGGAC	3 cut(s)	942, 1130, 1367
BslI	CCNNNNNNNGG	6 cut(s)	32, 147, 148, 727, 745, 1154
BsmAI	GTCTC	2 cut(s)	73, 287
BsmFI	GGGAC	3 cut(s)	942, 1130, 1367
BsmI	GAATGC	1 cut(s)	365
BsnI	GGCC	7 cut(s)	237, 720, 983, 1573, 1644, 1727, 2160
Bso31I	GGTCTC	2 cut(s)	73, 287
Bsp1286I	GDGCHC	2 cut(s)	1069, 1869
Bsp13I	TCCGGA	1 cut(s)	1459
Bsp143I	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
Bsp19I	CCATGG	2 cut(s)	978, 1495
BspACI	CCGC	7 cut(s)	33, 53, 102, 468, 1106, 1769, 2076
BspANI	GGCC	7 cut(s)	237, 720, 983, 1573, 1644, 1727, 2160
BspCNI	CTCAG	4 cut(s)	1714, 1837, 1903, 2061
BspDI	ATCGAT	1 cut(s)	819
BspEI	TCCGGA	1 cut(s)	1459
BspFNI	CGCG	1 cut(s)	209
BspLI	GGNNCC	8 cut(s)	472, 958, 1145, 1146, 1689, 2131, 2137, 2161
BspMI	ACCTGC	1 cut(s)	1319
BspPI	GGATC	3 cut(s)	1097, 1274, 1653
BspQI	GCTCTTC	1 cut(s)	1176
BspT107I	GGYRCC	2 cut(s)	470, 2135
BspTNI	GGTCTC	2 cut(s)	73, 287
BsrFI	RCCGGY	2 cut(s)	265, 716
BsrI	ACTGG	4 cut(s)	351, 984, 1069, 1389
BssAI	RCCGGY	2 cut(s)	265, 716
BssECI	CCNNGG	6 cut(s)	147, 202, 960, 978, 1495, 1728
BssHII	GCGCGC	1 cut(s)	207
BssMI	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
BssNI	GRCGYC	2 cut(s)	36, 634
BssSI	CACGAG	2 cut(s)	1273, 1567
BssT1I	CCWWGG	3 cut(s)	960, 978, 1495
Bst2BI	CACGAG	2 cut(s)	1273, 1567
Bst2UI	CCWGG	1 cut(s)	148
Bst4CI	ACNGT	4 cut(s)	163, 475, 956, 1603
Bst6I	CTCTTC	1 cut(s)	1176
BstACI	GRCGYC	2 cut(s)	36, 634
BstBAI	YACGTR	2 cut(s)	826, 884
BstC8I	GCNNGC	7 cut(s)	209, 317, 718, 1087, 1091, 2066, 2113
BstDEI	CTNAG	4 cut(s)	1722, 1845, 1911, 2069
BstDSI	CCRYGG	3 cut(s)	978, 1495, 1728
BstEII	GGTNACC	1 cut(s)	1126
BstFNI	CGCG	1 cut(s)	209
BstHHI	GCGC	4 cut(s)	209, 211, 247, 1826
BstKTI	GATC	7 cut(s)	301, 683, 1105, 1282, 1290, 1661, 2154
BstMAI	GTCTC	2 cut(s)	73, 287
BstMBI	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
BstMCI	CGRYCG	1 cut(s)	721
BstMWI	GCNNNNNNNGC	4 cut(s)	608, 980, 1217, 2054
BstNI	CCWGG	1 cut(s)	148
BstNSI	RCATGY	3 cut(s)	1093, 1511, 1775
BstPI	GGTNACC	1 cut(s)	1126
BstSCI	CCNGG	3 cut(s)	146, 720, 1350
BstSNI	TACGTA	1 cut(s)	826
BstUI	CGCG	1 cut(s)	209
BstV1I	GCAGC	2 cut(s)	241, 1337
BstV2I	GAAGAC	3 cut(s)	244, 677, 1491
BstX2I	RGATCY	2 cut(s)	1102, 1658
BstXI	CCANNNNNNTGG	3 cut(s)	413, 452, 2123
BstYI	RGATCY	2 cut(s)	1102, 1658
BstZI	CGGCCG	1 cut(s)	718
Bsu15I	ATCGAT	1 cut(s)	819
BsuI	GTATCC	1 cut(s)	1340
BsuRI	GGCC	7 cut(s)	237, 720, 983, 1573, 1644, 1727, 2160
BsuTUI	ATCGAT	1 cut(s)	819
BtgI	CCRYGG	3 cut(s)	978, 1495, 1728
BtsI	GCAGTG	3 cut(s)	177, 1348, 1747
BtsIMutI	CAGTG	5 cut(s)	177, 344, 1348, 1396, 1747
BveI	ACCTGC	1 cut(s)	1319
Cac8I	GCNNGC	7 cut(s)	209, 317, 718, 1087, 1091, 2066, 2113
CfoI	GCGC	4 cut(s)	209, 211, 247, 1826
Cfr10I	RCCGGY	2 cut(s)	265, 716
Cfr13I	GGNCC	6 cut(s)	956, 1144, 1331, 1492, 1642, 2159
ClaI	ATCGAT	1 cut(s)	819
CseI	GACGC	3 cut(s)	25, 642, 795
Csp6I	GTAC	4 cut(s)	217, 471, 575, 1425
CviQI	GTAC	4 cut(s)	217, 471, 575, 1425
DdeI	CTNAG	4 cut(s)	1722, 1845, 1911, 2069
DpnI	GATC	7 cut(s)	300, 682, 1104, 1281, 1289, 1660, 2153
DpnII	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
DraI	TTTAAA	1 cut(s)	1873
EaeI	YGGCCR	2 cut(s)	718, 981
EagI	CGGCCG	1 cut(s)	718
Eam1104I	CTCTTC	1 cut(s)	1176
EarI	CTCTTC	1 cut(s)	1176
EciI	GGCGGA	1 cut(s)	1095
EclXI	CGGCCG	1 cut(s)	718
Eco105I	TACGTA	1 cut(s)	826
Eco130I	CCWWGG	3 cut(s)	960, 978, 1495
Eco147I	AGGCCT	1 cut(s)	1573
Eco24I	GRGCYC	1 cut(s)	1069
Eco31I	GGTCTC	2 cut(s)	73, 287
Eco47I	GGWCC	4 cut(s)	956, 1144, 1331, 1492
Eco52I	CGGCCG	1 cut(s)	718
Eco57I	CTGAAG	1 cut(s)	1079
Eco72I	CACGTG	1 cut(s)	884
Eco91I	GGTNACC	1 cut(s)	1126
EcoO109I	RGGNCCY	2 cut(s)	1144, 2159
EcoO65I	GGTNACC	1 cut(s)	1126
EcoRII	CCWGG	1 cut(s)	146
EcoT14I	CCWWGG	3 cut(s)	960, 978, 1495
EcoT22I	ATGCAT	1 cut(s)	1509
EcoT38I	GRGCYC	1 cut(s)	1069
ErhI	CCWWGG	3 cut(s)	960, 978, 1495
FalI	AAGNNNNNCTT	2 cut(s)	1132, 1164
FaqI	GGGAC	3 cut(s)	942, 1130, 1367
FauI	CCCGC	1 cut(s)	60
FauNDI	CATATG	1 cut(s)	1683
FbaI	TGATCA	2 cut(s)	298, 1287
FblI	GTMKAC	1 cut(s)	39
Fnu4HI	GCNGC	4 cut(s)	34, 103, 230, 1326
FriOI	GRGCYC	1 cut(s)	1069
Fsp4HI	GCNGC	4 cut(s)	34, 103, 230, 1326
FspBI	CTAG	5 cut(s)	810, 920, 1221, 1299, 1551
GlaI	GCGC	4 cut(s)	208, 210, 246, 1825
GluI	GCNGC	4 cut(s)	34, 103, 230, 1326
GsuI	CTGGAG	1 cut(s)	130
HaeIII	GGCC	7 cut(s)	237, 720, 983, 1573, 1644, 1727, 2160
HapII	CCGG	7 cut(s)	266, 717, 721, 1319, 1351, 1460, 2133
HgaI	GACGC	3 cut(s)	25, 642, 795
HhaI	GCGC	4 cut(s)	209, 211, 247, 1826
Hin1I	GRCGYC	2 cut(s)	36, 634
Hin6I	GCGC	4 cut(s)	207, 209, 245, 1824
HinP1I	GCGC	4 cut(s)	207, 209, 245, 1824
HincII	GTYRAC	2 cut(s)	1270, 1906
HindII	GTYRAC	2 cut(s)	1270, 1906
HindIII	AAGCTT	1 cut(s)	1248
HinfI	GANTC	9 cut(s)	359, 673, 749, 909, 1042, 1241, 1907, 1979, 2185
HpaII	CCGG	7 cut(s)	266, 717, 721, 1319, 1351, 1460, 2133
HphI	GGTGA	9 cut(s)	191, 370, 395, 432, 1169, 1457, 1529, 2006, 2090
Hpy166II	GTNNAC	7 cut(s)	40, 271, 520, 935, 1270, 1334, 1906
Hpy188I	TCNGA	9 cut(s)	328, 591, 685, 914, 1651, 1816, 1846, 1912, 2032
Hpy188III	TCNNGA	7 cut(s)	188, 478, 739, 950, 1007, 1460, 2189
Hpy8I	GTNNAC	7 cut(s)	40, 271, 520, 935, 1270, 1334, 1906
Hpy99I	CGWCG	2 cut(s)	80, 163
HpyAV	CCTTC	7 cut(s)	143, 580, 1392, 1420, 1535, 1584, 2150
HpyCH4III	ACNGT	4 cut(s)	163, 475, 956, 1603
HpyCH4IV	ACGT	5 cut(s)	801, 825, 883, 1761, 1863
HpyCH4V	TGCA	7 cut(s)	170, 365, 1325, 1507, 1655, 1837, 2111
HpyF10VI	GCNNNNNNNGC	4 cut(s)	608, 980, 1217, 2054
HpyF3I	CTNAG	4 cut(s)	1722, 1845, 1911, 2069
HpySE526I	ACGT	5 cut(s)	801, 825, 883, 1761, 1863
Hsp92I	GRCGYC	2 cut(s)	36, 634
HspAI	GCGC	4 cut(s)	207, 209, 245, 1824
KflI	GGGWCCC	1 cut(s)	1144
Kpn2I	TCCGGA	1 cut(s)	1459
KpnI	GGTACC	1 cut(s)	474
KroI	GCCGGC	1 cut(s)	716
KroNI	GCCGGC	1 cut(s)	718
Ksp22I	TGATCA	2 cut(s)	298, 1287
Kzo9I	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
LguI	GCTCTTC	1 cut(s)	1176
LmnI	GCTCC	5 cut(s)	952, 1162, 1499, 1693, 1786
Lsp1109I	GCAGC	2 cut(s)	241, 1337
MaeI	CTAG	5 cut(s)	810, 920, 1221, 1299, 1551
MaeII	ACGT	5 cut(s)	801, 825, 883, 1761, 1863
MaeIII	GTNAC	6 cut(s)	19, 1126, 1807, 1859, 1994, 2086
MalI	GATC	7 cut(s)	300, 682, 1104, 1281, 1289, 1660, 2153
MboI	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
MboII	GAAGA	9 cut(s)	244, 493, 682, 778, 1190, 1193, 1217, 1496, 1805
MfeI	CAATTG	2 cut(s)	1800, 1838
MflI	RGATCY	2 cut(s)	1102, 1658
MhlI	GDGCHC	2 cut(s)	1069, 1869
MlsI	TGGCCA	1 cut(s)	983
MluNI	TGGCCA	1 cut(s)	983
MlyI	GAGTC	2 cut(s)	667, 1901
MmeI	TCCRAC	3 cut(s)	737, 1861, 2055
Mox20I	TGGCCA	1 cut(s)	983
Mph1103I	ATGCAT	1 cut(s)	1509
MroI	TCCGGA	1 cut(s)	1459
MroNI	GCCGGC	1 cut(s)	716
MscI	TGGCCA	1 cut(s)	983
MseI	TTAA	9 cut(s)	386, 558, 897, 1122, 1361, 1853, 1872, 2000, 2221
MslI	CAYNNNNRTG	3 cut(s)	1512, 1776, 2121
Msp20I	TGGCCA	1 cut(s)	983
MspA1I	CMGCKG	2 cut(s)	55, 1721
MspI	CCGG	7 cut(s)	266, 717, 721, 1319, 1351, 1460, 2133
MspR9I	CCNGG	3 cut(s)	148, 722, 1352
MunI	CAATTG	2 cut(s)	1800, 1838
Mva1269I	GAATGC	1 cut(s)	365
MvaI	CCWGG	1 cut(s)	148
MvnI	CGCG	1 cut(s)	209
MwoI	GCNNNNNNNGC	4 cut(s)	608, 980, 1217, 2054
NaeI	GCCGGC	1 cut(s)	718
NciI	CCSGG	2 cut(s)	722, 1352
NcoI	CCATGG	2 cut(s)	978, 1495
NdeI	CATATG	1 cut(s)	1683
NdeII	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
NgoMIV	GCCGGC	1 cut(s)	716
NlaIV	GGNNCC	8 cut(s)	472, 958, 1145, 1146, 1689, 2131, 2137, 2161
NmeAIII	GCCGAG	2 cut(s)	183, 213
NmuCI	GTSAC	2 cut(s)	1994, 2086
NsiI	ATGCAT	1 cut(s)	1509
NspI	RCATGY	3 cut(s)	1093, 1511, 1775
PaeI	GCATGC	1 cut(s)	1093
PauI	GCGCGC	1 cut(s)	207
PceI	AGGCCT	1 cut(s)	1573
PciSI	GCTCTTC	1 cut(s)	1176
PcsI	WCGNNNNNNNCGW	1 cut(s)	117
PctI	GAATGC	1 cut(s)	365
PdiI	GCCGGC	1 cut(s)	718
PfeI	GAWTC	7 cut(s)	359, 749, 909, 1042, 1241, 1979, 2185
Pfl23II	CGTACG	2 cut(s)	216, 574
PflMI	CCANNNNNTGG	1 cut(s)	745
PkrI	GCNGC	4 cut(s)	35, 104, 231, 1327
PleI	GAGTC	2 cut(s)	667, 1901
PmaCI	CACGTG	1 cut(s)	884
PmlI	CACGTG	1 cut(s)	884
PpsI	GAGTC	2 cut(s)	667, 1901
Ppu21I	YACGTR	2 cut(s)	826, 884
PpuMI	RGGWCCY	1 cut(s)	1144
PshBI	ATTAAT	1 cut(s)	558
PsiI	TTATAA	2 cut(s)	1454, 1830
Psp5II	RGGWCCY	1 cut(s)	1144
Psp6I	CCWGG	1 cut(s)	146
PspCI	CACGTG	1 cut(s)	884
PspEI	GGTNACC	1 cut(s)	1126
PspGI	CCWGG	1 cut(s)	146
PspLI	CGTACG	2 cut(s)	216, 574
PspN4I	GGNNCC	8 cut(s)	472, 958, 1145, 1146, 1689, 2131, 2137, 2161
PspPI	GGNCC	6 cut(s)	956, 1144, 1331, 1492, 1642, 2159
PspPPI	RGGWCCY	1 cut(s)	1144
PsrI	GAACNNNNNNTAC	2 cut(s)	287, 319
PsuI	RGATCY	2 cut(s)	1102, 1658
PteI	GCGCGC	1 cut(s)	207
PvuII	CAGCTG	1 cut(s)	1721
RsaI	GTAC	4 cut(s)	218, 472, 576, 1426
RsaNI	GTAC	4 cut(s)	217, 471, 575, 1425
RseI	CAYNNNNRTG	3 cut(s)	1512, 1776, 2121
SapI	GCTCTTC	1 cut(s)	1176
SaqAI	TTAA	9 cut(s)	386, 558, 897, 1122, 1361, 1853, 1872, 2000, 2221
SatI	GCNGC	4 cut(s)	34, 103, 230, 1326
Sau3AI	GATC	7 cut(s)	298, 680, 1102, 1279, 1287, 1658, 2151
Sau96I	GGNCC	6 cut(s)	956, 1144, 1331, 1492, 1642, 2159
SchI	GAGTC	2 cut(s)	667, 1901
ScrFI	CCNGG	3 cut(s)	148, 722, 1352
SduI	GDGCHC	2 cut(s)	1069, 1869
SinI	GGWCC	4 cut(s)	956, 1144, 1331, 1492
SmiMI	CAYNNNNRTG	3 cut(s)	1512, 1776, 2121
SmlI	CTYRAG	1 cut(s)	839
SmoI	CTYRAG	1 cut(s)	839
SnaBI	TACGTA	1 cut(s)	826
SphI	GCATGC	1 cut(s)	1093
SseBI	AGGCCT	1 cut(s)	1573
SsiI	CCGC	7 cut(s)	33, 53, 102, 468, 1106, 1769, 2076
SspMI	CTAG	5 cut(s)	810, 920, 1221, 1299, 1551
StuI	AGGCCT	1 cut(s)	1573
StyD4I	CCNGG	3 cut(s)	146, 720, 1350
StyI	CCWWGG	3 cut(s)	960, 978, 1495
TaaI	ACNGT	4 cut(s)	163, 475, 956, 1603
TaiI	ACGT	5 cut(s)	804, 828, 886, 1764, 1866
TaqI	TCGA	8 cut(s)	75, 96, 120, 158, 532, 819, 1589, 2154
TauI	GCSGC	2 cut(s)	36, 105
TfiI	GAWTC	7 cut(s)	359, 749, 909, 1042, 1241, 1979, 2185
Tru1I	TTAA	9 cut(s)	386, 558, 897, 1122, 1361, 1853, 1872, 2000, 2221
Tru9I	TTAA	9 cut(s)	386, 558, 897, 1122, 1361, 1853, 1872, 2000, 2221
TscAI	CASTG	5 cut(s)	177, 351, 1348, 1396, 1754
TseFI	GTSAC	2 cut(s)	1994, 2086
TseI	GCWGC	2 cut(s)	229, 1325
Tsp45I	GTSAC	2 cut(s)	1994, 2086
TspDTI	ATGAA	7 cut(s)	239, 1191, 1497, 1554, 1608, 1700, 1992
TspGWI	ACGGA	3 cut(s)	204, 567, 1745
TspRI	CASTG	5 cut(s)	177, 351, 1348, 1396, 1754
Van91I	CCANNNNNTGG	1 cut(s)	745
VpaK11BI	GGWCC	4 cut(s)	956, 1144, 1331, 1492
VspI	ATTAAT	1 cut(s)	558
XapI	RAATTY	4 cut(s)	373, 650, 769, 2051
XceI	RCATGY	3 cut(s)	1093, 1511, 1775
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2123
XmiI	GTMKAC	1 cut(s)	39
XspI	CTAG	5 cut(s)	810, 920, 1221, 1299, 1551
Zsp2I	ATGCAT	1 cut(s)	1509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.