Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0006213

Physical Location & Seq

Genomic Coordinates Reverse (-)

3486298 .. 3496337

Gene Structure 10040 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00281130.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3489 bp

ATGGGGTCTATATGGAATCTTTTGTATTTGCTGGAGCCTGCACCTGTTTCTCTAATTCTAACGGCAGTAGGGGTTACATTTGGGTCTGCATTTCGTGCTCTAAATTATGGAAAAGAAATGGAGCGAAATCGTGATTTTTCAGAAGCATCGATTACGTTAGATAGGTCCCAAGCGCTTATGATCCCTGTAATGAGCTCCCTAAGCTTGCTTTTGATGTTCTACCTCTTCACTTCTGTTTCACAACTCCTGACTGTATTCACAGCCATTGCATCTGTCTCCTCCCTGTTTTTCTGCCTATCTCCTTCAGTAGCCTATTTGAAGTCTCAGCTTGGTTTGCCCGACCCCTATGTGTCTAGGTGTTGTTCAAAGTCATTTACACGCACCCAAGGATCGTTACTGGTTTTATGCATTGGTACAGTTGCTACTTGGCTTGTTACTGGACATTGGATACTGAACAACTTGTTGGGCATTTCCATATGTATTGCCTTTGTGAGTCATGTCCGTCTTCCAAACATCAAAGTTTGTGCAATGCTCCTTGTCTGTCTATTCGTATACGACATATTCTGGGTCTTCTTCTCTGAGAGATTTTTTGGGGCGAATGTCATGGTATCGGTAGCAACACAACAAGCCTCAAACCCTGTTCACACGGTGGCAAATAGTTTGAGTCTTCCTGGGCTGCAAATGGTGACAAAGAAGTTGGAGTTGCCTGTGAAGATTGTTTTCCCCAGGAACTTATTTGGTGGAGTGGCTCCTGGAGGAAGTACCAGAGACTTCATGATGCTCGGTCTGGGTGACATGGCAATCCCTGCTATGCTTCTGGCATTAGTCCTATGTTTTGATTATCGGAAGAGCAGGGACACAGTAAATTTGCTAGAGATGCATTCCTCAAAGGGGCACAAATATATTTGGTATGCCCTGCCTGGATATGCCTTTGGACTGGTCACAGCTTTAGCAGCTGGAGTTTTGACGCAGTCACCCCAACCTGCTCTTCTCTATTTGGTGCCCTCAACGCTAGGACCAATTGTCGTCATTTCTTGGATTAGGAAGGAACTAGCAGAATTATGGGATGGCCCTTTGCCAAGCTTCAACGATAAAGTTCACCAGATAGAAGCAGGTGTATTATCAAAGATGAGAAAATCTGTCATGAGAAAATCGTTTCAAGAGCTCGATGACCTTGATATTCCATCAGAAGCTGAATCAGAAACATATATGGTCCTCAATGACTGGATGCTAGAATGCACACACAGAGAGAGTGAGAGAGAGACGATGGGAACTTCGGTTCAGGTAACGCCGCTGTGCGGAGTCTACAACGAGAATCCACTCTCCTATTTGGTCTCCATCGATGGCTTCAACTTCCTCATCGACTGTGGCTGGAACGACCACTTCGACCCCTCCCTCCTCCAACCTCTCTCCAGAGTCGCTTCCACCGTAGACGCAGTCCTACTGTCCCACCCCGACACACTCCACCTCGGCGCGCTTCCCTACGCCGCGAAACACCTCGGCCTTGCCGCTCCGGTCTTCTCCACCGAGCCGGTTTACCGGCTAGGGCTTCTCACTATGTACGATCAGTACCTCTCCCGCAAGCAAGTTTCGGAGTTCGATTTGTTTACGCTGGATGATATTGATTCTGCTTTCCAAAATGTCACCAGGCTGACCAATGCTCAGCATCATCATCTTACGGGCAAAGGAGAGGGAATTGTGATCTCGCCTCATGTGGCTGGGCATCTATTGGGAGGTACTGTGTGGAAGATAACAAAGGATGGAGAGGATGTTATATATGCTGTGGACTTCAACCATCGAAAAGAAAAGCATTTGAATGGAATTAATCAGTCATCATTTGTGCGGCCTGCTGTTCTAATAACAGATGCCTATAATGCTTTGAACAATCAGCCTTATAGACGCCAGAAGGACAGAGAATTTACAGAGACTATAAAGAAGACTCTACGATCTCAAGGAAATGTTTTACTACCTGTTGATACCGCTGGGCGAGTTTTGGAACTTCTTCAAATATTGGAATCGTGTTGGAACGAGGAAAGTTTGCCCTTCCCCATCTATTTCCTAACGTATGTTGCTTCTAGCACAATTGATTATGTCAAAAGTTTCCTTGAGTGGATGAGTGATGCAATGGCAAAGTCCTTTGAGACAACACGAGACAATGCTTTTATTTTGAAGCGTGTGAAACTTCTTGTTAACAAGAGCGAACTTGACAATGCTCCGGAAGGCCCAAAGGTTGTTTTAGCATCCATGGCCAGTTTGGAAGCAGGTTTTTCTCATGATATATTTGTCGAATGGGCAACTGATGCTAAGAATCTTGTGCTTTTTACAGAACGAGCTCAGTTTGGCACGTTAGCTCGTATGCTTCAGGCAGACCCGCCTCCAAAAGCTGTCAAGGTTACCATGTCCAAGAGGATCCCTTTGGTTGGAGAAGAGCTAATTGCTTATGAAGAAGAGCAGAACAGAATTAAAAAGGAAGAAGCTCTGAAGGCTAGTCTGATTAAAGAGGAGGAGTTAAAAGCTTCTCATGGGGCTGATGTCAGCATGAGTGATCCACTGGTTATTGATACTAGCAATGCAAAGTCTTTGCCAGATGCAGTGGCTCCACGTGGTGGTGGATGCAGAGACATTTTAATCGATGGATTTACTCCTCCTTCTACTAGTGTTGCCCCTATGTTTCCCTTTTATGAAAATAATTCTGAGTGGGATGACTTTGGTGAAGTTATAAATCCGGACGATTATGTTATCAAGGATGAAGACATGAACCAAGGAGCAATGCTTGTTGGAGGTGATATGGATGGAAAAATTGACGAAGCCTCTGCTAGTTTAATACTCGACTCAAGGCCTTCGAAAGTTGTGTCAAGTGAATTGACTGTACCAGTGAAGTGTTCACTGATATATATGGACTTTGAGGGCCGTTCTGATGCGAGATCTGTTAAATCGATTCTTTCGCATATGGCTCCTCTAAAGCTTGTTTTGGTTCATGGAACAGCTGAGGCCACTGAACATTTGAAGCAACACTGCTTGAAACATGTTTGCCCTCATGTATATGCTCCTCAACTTGAAGAAACAATTGATGTCACATCTGATTTATGTGCTTATAAGGTACAACTGTCCGAGAAGCTCATGAGTAACATAATCTTTAAGAAGCTTGGAGAGAATGAAATAGCTTGGTTCGATTCTGATGTAGGGAAGACAGAAGACGAAATGCTGACTTTACTACCCTGCTCAACCCCAGCTCCACCACACAAACCAATCCTAGTTGGTGACTTAAAAATGGCCGATTTCAAGCAATTTCTTGCTGATAACAGTGTCCAGGTTGAATTTGCCGGTGGGGCTTTGCGATGTGGTGAATATGTGACAATACGCAAGGTTGGGGATGCAAGCCATAAGGGTGGCGGTGCTGGTTCGCAGCAAATTGTGATTGAAGGTCCAGCATGTGAGGATTATTACAAGATACGAGAATATCTCTATTCGCACTTTTATTTACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005847 GO:0005849 GO:0005911 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0030054 GO:0031047 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0055044 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Beta-Casp CPSF100_C Lactamase_B_6 RMMBL

Protein Analysis

1162

Amino Acids

128.76

Weight (kDa)

5.42

Isoelectric Point (pI)

40.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_A22B	PF04258	49 - 357	2.5e-72	Signal peptide peptidase
Lactamase_B	PF00753	441 - 568	3.8e-07	Metallo-beta-lactamase superfamily
Lactamase_B_6	PF16661	444 - 616	4.1e-53	Metallo-beta-lactamase superfamily domain
Beta-Casp	PF10996	664 - 787	8.4e-23	Beta-Casp domain
RMMBL	PF07521	957 - 1007	5.2e-10	Zn-dependent metallo-hydrolase RNA specificity domain
CPSF100_C	PF13299	1066 - 1159	2.1e-18	Cleavage and polyadenylation factor 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006213)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23880
fragaria_vesca	FvH4_5g14450 FvH4_7g29660 FvH4_7g29660
malus_domestica	MD01G1203600.v1.1 MD07G1272900.v1.1
prunus_persica	Prupe.2G296200_v2.0.a1 Prupe.2G296200_v2.0.a1
pyrus_communis	pycom01g21270 pycom01g21280 pycom07g24930
rosa_chinensis	RchiOBHm_Chr1g0377671 RchiOBHm_Chr1g0377681 RchiOBHm_Chr1g0379161 RchiOBHm_Chr1g0379331
rosa_laevigata	RLG00000026506 RLG00000026773
rosa_multiflora	Rmu_sc0002832.1_g000015
rosa_roxburghii	Rroxscaffold_4G00281130
rosa_rugosa	Rorug01G0404200 Rorug01G0404300
rosa_samantha	Rh1AG428300 Rh1BG381000 Rh1CG000300 Rh1CG395000 Rh1DG412000
rosa_wichuraiana	Rw1G037040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2720, 3096
AarI	CACCTGC	1 cut(s)	1103
Acc36I	ACCTGC	3 cut(s)	991, 1103, 2252
AccB1I	GGYRCC	1 cut(s)	1000
AccB7I	CCANNNNNTGG	1 cut(s)	2606
AccBSI	CCGCTC	1 cut(s)	1511
AccI	GTMKAC	3 cut(s)	552, 1305, 1431
AccII	CGCG	2 cut(s)	1475, 1490
AccIII	TCCGGA	2 cut(s)	2215, 2725
AciI	CCGC	9 cut(s)	1292, 1299, 1488, 1509, 1579, 1843, 1980, 2372, 3393
AclWI	GGATC	5 cut(s)	175, 397, 2404, 2417, 2540
AcoI	YGGCCR	2 cut(s)	2247, 3273
AcsI	RAATTY	3 cut(s)	865, 1916, 3317
AcuI	CTGAAG	3 cut(s)	288, 2345, 2501
AcvI	CACGTG	1 cut(s)	2603
AcyI	GRCGYC	1 cut(s)	1900
AdeI	CACNNNGTG	2 cut(s)	649, 2606
AfaI	GTAC	7 cut(s)	415, 763, 1562, 1571, 1738, 2871, 3102
AfeI	AGCGCT	1 cut(s)	174
AfiI	CCNNNNNNNGG	4 cut(s)	891, 1298, 2420, 2606
AflIII	ACRYGT	1 cut(s)	3025
AhdI	GACNNNNNGTC	1 cut(s)	1022
AhlI	ACTAGT	1 cut(s)	2654
AjnI	CCWGG	6 cut(s)	670, 725, 751, 919, 1646, 3309
AjuI	GAANNNNNNNTTGG	4 cut(s)	446, 478, 972, 1004
AloI	GAACNNNNNNTCC	2 cut(s)	1263, 1295
Alw21I	GWGCWC	4 cut(s)	100, 197, 1167, 2335
Alw26I	GTCTC	9 cut(s)	280, 327, 762, 1256, 1339, 1919, 2135, 2145, 2613
AlwI	GGATC	5 cut(s)	175, 397, 2404, 2417, 2540
AlwNI	CAGNNNCTG	1 cut(s)	1193
Aor13HI	TCCGGA	2 cut(s)	2215, 2725
Aor51HI	AGCGCT	1 cut(s)	174
AoxI	GGCC	9 cut(s)	1069, 1501, 1844, 2221, 2247, 2837, 2908, 2991, 3273
ApeKI	GCWGC	3 cut(s)	676, 953, 3406
ApoI	RAATTY	3 cut(s)	865, 1916, 3317
ArsI	GACNNNNNNTTYG	2 cut(s)	2837, 2869
AseI	ATTAAT	1 cut(s)	1824
Asp700I	GAANNNNTTC	1 cut(s)	2001
AspLEI	GCGC	3 cut(s)	175, 1475, 1477
AspS9I	GGNCC	7 cut(s)	165, 1016, 1070, 1213, 2222, 2908, 3425
AsuHPI	GGTGA	9 cut(s)	697, 803, 966, 1091, 1636, 2723, 2795, 3272, 3356
AsuII	TTCGAA	1 cut(s)	2843
AvaII	GGWCC	4 cut(s)	165, 1016, 1213, 3425
BaeGI	GKGCMC	2 cut(s)	897, 1005
BaeI	ACNNNNGTAYC	2 cut(s)	3092, 3125
BalI	TGGCCA	1 cut(s)	2249
BamHI	GGATCC	1 cut(s)	2409
BanI	GGYRCC	1 cut(s)	1000
BanII	GRGCYC	3 cut(s)	197, 1167, 2335
BauI	CACGAG	1 cut(s)	2148
BbrPI	CACGTG	1 cut(s)	2603
BbsI	GAAGAC	8 cut(s)	497, 562, 659, 1510, 1943, 2757, 3194, 3201
Bbv12I	GWGCWC	4 cut(s)	100, 197, 1167, 2335
BbvCI	CCTCAGC	1 cut(s)	2988
BbvI	GCAGC	3 cut(s)	663, 965, 3418
BceAI	ACGGC	2 cut(s)	78, 2895
BcgI	CGANNNNNNTGC	2 cut(s)	2795, 2829
BciT130I	CCWGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
BciVI	GTATCC	1 cut(s)	441
BcoDI	GTCTC	9 cut(s)	280, 327, 762, 1256, 1339, 1919, 2135, 2145, 2613
BcuI	ACTAGT	1 cut(s)	2654
BfoI	RGCGCY	1 cut(s)	176
BfuAI	ACCTGC	3 cut(s)	991, 1103, 2252
BfuI	GTATCC	1 cut(s)	441
BglI	GCCNNNNNGGC	1 cut(s)	3329
BglII	AGATCT	1 cut(s)	2924
BisI	GCNGC	7 cut(s)	677, 954, 1292, 1488, 1509, 1844, 3407
BlpI	GCTNAGC	1 cut(s)	1662
BlsI	GCNGC	7 cut(s)	678, 955, 1293, 1489, 1510, 1845, 3408
Bme1390I	CCNGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
Bme18I	GGWCC	4 cut(s)	165, 1016, 1213, 3425
BmeRI	GACNNNNNGTC	1 cut(s)	1022
BmgT120I	GGNCC	7 cut(s)	165, 1016, 1070, 1213, 2222, 2908, 3425
BmiI	GGNNCC	7 cut(s)	36, 167, 750, 1002, 2411, 2598, 2955
BmrFI	CCNGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
BpiI	GAAGAC	8 cut(s)	497, 562, 659, 1510, 1943, 2757, 3194, 3201
BplI	GAGNNNNNCTC	2 cut(s)	3447, 3479
BpmI	CTGGAG	4 cut(s)	53, 774, 978, 1396
Bpu10I	CCTNAGC	2 cut(s)	200, 2988
Bpu1102I	GCTNAGC	1 cut(s)	1662
Bpu14I	TTCGAA	1 cut(s)	2843
BpuEI	CTTGAG	3 cut(s)	1935, 2126, 2818
Bsa29I	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
BsaAI	YACGTR	1 cut(s)	2603
BsaHI	GRCGYC	1 cut(s)	1900
BsaI	GGTCTC	1 cut(s)	1339
BsaJI	CCNNGG	7 cut(s)	385, 671, 725, 1468, 1498, 2244, 2761
BsaWI	WCCGGW	3 cut(s)	1513, 2215, 2725
BsaXI	ACNNNNNCTCC	6 cut(s)	1725, 1755, 1755, 1785, 3217, 3247
Bsc4I	CCNNNNNNNGG	4 cut(s)	891, 1298, 2420, 2606
Bse118I	RCCGGY	3 cut(s)	1531, 1539, 3323
Bse1I	ACTGG	7 cut(s)	402, 442, 942, 1229, 2250, 2556, 2873
Bse3DI	GCAATG	5 cut(s)	264, 534, 2130, 2575, 2775
BseAI	TCCGGA	2 cut(s)	2215, 2725
BseBI	CCWGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
BseCI	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
BseDI	CCNNGG	7 cut(s)	385, 671, 725, 1468, 1498, 2244, 2761
BseLI	CCNNNNNNNGG	4 cut(s)	891, 1298, 2420, 2606
BseMI	GCAATG	5 cut(s)	264, 534, 2130, 2575, 2775
BseMII	CTCAG	6 cut(s)	338, 570, 1676, 2348, 2685, 2979
BseNI	ACTGG	7 cut(s)	402, 442, 942, 1229, 2250, 2556, 2873
BsePI	GCGCGC	1 cut(s)	1473
BseRI	GAGGAG	7 cut(s)	268, 1388, 2516, 2519, 2634, 2946, 3039
BseSI	GKGCMC	2 cut(s)	897, 1005
BseXI	GCAGC	3 cut(s)	663, 965, 3418
BseYI	CCCAGC	3 cut(s)	1718, 1982, 3229
BsgI	GTGCAG	1 cut(s)	24
Bsh1236I	CGCG	2 cut(s)	1475, 1490
BshFI	GGCC	9 cut(s)	1071, 1503, 1846, 2223, 2249, 2839, 2910, 2993, 3275
BshNI	GGYRCC	1 cut(s)	1000
BshVI	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
BsiHKAI	GWGCWC	4 cut(s)	100, 197, 1167, 2335
BsiSI	CCGG	6 cut(s)	1514, 1532, 1540, 2216, 2726, 3324
BslFI	GGGAC	3 cut(s)	151, 869, 1432
BslI	CCNNNNNNNGG	4 cut(s)	891, 1298, 2420, 2606
BsmAI	GTCTC	9 cut(s)	280, 327, 762, 1256, 1339, 1919, 2135, 2145, 2613
BsmBI	CGTCTC	1 cut(s)	1256
BsmFI	GGGAC	3 cut(s)	151, 869, 1432
BsmI	GAATGC	2 cut(s)	880, 1241
BsnI	GGCC	9 cut(s)	1071, 1503, 1846, 2223, 2249, 2839, 2910, 2993, 3275
Bso31I	GGTCTC	1 cut(s)	1339
Bsp119I	TTCGAA	1 cut(s)	2843
Bsp1286I	GDGCHC	6 cut(s)	100, 197, 897, 1005, 1167, 2335
Bsp13I	TCCGGA	2 cut(s)	2215, 2725
Bsp143I	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
Bsp1720I	GCTNAGC	1 cut(s)	1662
Bsp19I	CCATGG	1 cut(s)	2244
BspACI	CCGC	9 cut(s)	1292, 1299, 1488, 1509, 1579, 1843, 1980, 2372, 3393
BspANI	GGCC	9 cut(s)	1071, 1503, 1846, 2223, 2249, 2839, 2910, 2993, 3275
BspCNI	CTCAG	6 cut(s)	337, 571, 1675, 2347, 2686, 2980
BspDI	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
BspEI	TCCGGA	2 cut(s)	2215, 2725
BspFNI	CGCG	2 cut(s)	1475, 1490
BspHI	TCATGA	4 cut(s)	774, 1143, 2272, 3120
BspLI	GGNNCC	7 cut(s)	36, 167, 750, 1002, 2411, 2598, 2955
BspMI	ACCTGC	3 cut(s)	991, 1103, 2252
BspPI	GGATC	5 cut(s)	175, 397, 2404, 2417, 2540
BspQI	GCTCTTC	4 cut(s)	842, 993, 2421, 2442
BspT104I	TTCGAA	1 cut(s)	2843
BspT107I	GGYRCC	1 cut(s)	1000
BspTNI	GGTCTC	1 cut(s)	1339
BsrBI	CCGCTC	1 cut(s)	1511
BsrDI	GCAATG	5 cut(s)	264, 534, 2130, 2575, 2775
BsrFI	RCCGGY	3 cut(s)	1531, 1539, 3323
BsrI	ACTGG	7 cut(s)	402, 442, 942, 1229, 2250, 2556, 2873
BssAI	RCCGGY	3 cut(s)	1531, 1539, 3323
BssECI	CCNNGG	7 cut(s)	385, 671, 725, 1468, 1498, 2244, 2761
BssHII	GCGCGC	1 cut(s)	1473
BssMI	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
BssNAI	GTATAC	1 cut(s)	553
BssNI	GRCGYC	1 cut(s)	1900
BssSI	CACGAG	1 cut(s)	2148
BssT1I	CCWWGG	3 cut(s)	385, 2244, 2761
Bst1107I	GTATAC	1 cut(s)	553
Bst2BI	CACGAG	1 cut(s)	2148
Bst2UI	CCWGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
Bst6I	CTCTTC	5 cut(s)	230, 842, 993, 2421, 2442
BstACI	GRCGYC	1 cut(s)	1900
BstAPI	GCANNNNNTGC	3 cut(s)	95, 806, 2300
BstBAI	YACGTR	1 cut(s)	2603
BstBI	TTCGAA	1 cut(s)	2843
BstC8I	GCNNGC	6 cut(s)	39, 206, 1475, 1583, 1848, 3379
BstDEI	CTNAG	8 cut(s)	200, 324, 579, 1662, 2304, 2334, 2694, 2988
BstDSI	CCRYGG	1 cut(s)	2244
BstEII	GGTNACC	1 cut(s)	2392
BstFNI	CGCG	2 cut(s)	1475, 1490
BstH2I	RGCGCY	1 cut(s)	176
BstHHI	GCGC	3 cut(s)	175, 1475, 1477
BstKTI	GATC	8 cut(s)	183, 392, 1567, 1704, 1949, 2412, 2548, 2927
BstMAI	GTCTC	9 cut(s)	280, 327, 762, 1256, 1339, 1919, 2135, 2145, 2613
BstMBI	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
BstNI	CCWGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
BstNSI	RCATGY	2 cut(s)	3029, 3435
BstPI	GGTNACC	1 cut(s)	2392
BstSCI	CCNGG	6 cut(s)	670, 725, 751, 919, 1646, 3309
BstSLI	GKGCMC	2 cut(s)	897, 1005
BstUI	CGCG	2 cut(s)	1475, 1490
BstV1I	GCAGC	3 cut(s)	663, 965, 3418
BstV2I	GAAGAC	8 cut(s)	497, 562, 659, 1510, 1943, 2757, 3194, 3201
BstX2I	RGATCY	2 cut(s)	2409, 2924
BstXI	CCANNNNNNTGG	1 cut(s)	3389
BstYI	RGATCY	2 cut(s)	2409, 2924
BstZ17I	GTATAC	1 cut(s)	553
Bsu15I	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
BsuI	GTATCC	1 cut(s)	441
BsuRI	GGCC	9 cut(s)	1071, 1503, 1846, 2223, 2249, 2839, 2910, 2993, 3275
BsuTUI	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
BtgI	CCRYGG	1 cut(s)	2244
BtgZI	GCGATG	1 cut(s)	3352
BtsI	GCAGTG	2 cut(s)	2598, 3013
BtsIMutI	CAGTG	7 cut(s)	2549, 2598, 2880, 2885, 2994, 3013, 3310
BveI	ACCTGC	3 cut(s)	991, 1103, 2252
Cac8I	GCNNGC	6 cut(s)	39, 206, 1475, 1583, 1848, 3379
CaiI	CAGNNNCTG	1 cut(s)	1193
CciI	TCATGA	4 cut(s)	774, 1143, 2272, 3120
CfoI	GCGC	3 cut(s)	175, 1475, 1477
Cfr10I	RCCGGY	3 cut(s)	1531, 1539, 3323
Cfr13I	GGNCC	7 cut(s)	165, 1016, 1070, 1213, 2222, 2908, 3425
ClaI	ATCGAT	4 cut(s)	149, 1341, 2631, 2936
CseI	GACGC	3 cut(s)	976, 1442, 1908
Csp6I	GTAC	7 cut(s)	414, 762, 1561, 1570, 1737, 2870, 3101
CviQI	GTAC	7 cut(s)	414, 762, 1561, 1570, 1737, 2870, 3101
DdeI	CTNAG	8 cut(s)	200, 324, 579, 1662, 2304, 2334, 2694, 2988
DpnI	GATC	8 cut(s)	182, 391, 1566, 1703, 1948, 2411, 2547, 2926
DpnII	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
DraIII	CACNNNGTG	2 cut(s)	649, 2606
DriI	GACNNNNNGTC	1 cut(s)	1022
EaeI	YGGCCR	2 cut(s)	2247, 3273
Eam1104I	CTCTTC	5 cut(s)	230, 842, 993, 2421, 2442
Eam1105I	GACNNNNNGTC	1 cut(s)	1022
EarI	CTCTTC	5 cut(s)	230, 842, 993, 2421, 2442
Ecl136II	GAGCTC	3 cut(s)	195, 1165, 2333
Eco130I	CCWWGG	3 cut(s)	385, 2244, 2761
Eco147I	AGGCCT	1 cut(s)	2839
Eco24I	GRGCYC	3 cut(s)	197, 1167, 2335
Eco31I	GGTCTC	1 cut(s)	1339
Eco47I	GGWCC	4 cut(s)	165, 1016, 1213, 3425
Eco47III	AGCGCT	1 cut(s)	174
Eco53kI	GAGCTC	3 cut(s)	195, 1165, 2333
Eco57I	CTGAAG	3 cut(s)	288, 2345, 2501
Eco72I	CACGTG	1 cut(s)	2603
Eco91I	GGTNACC	1 cut(s)	2392
EcoICRI	GAGCTC	3 cut(s)	195, 1165, 2333
EcoO109I	RGGNCCY	1 cut(s)	165
EcoO65I	GGTNACC	1 cut(s)	2392
EcoRII	CCWGG	6 cut(s)	670, 725, 751, 919, 1646, 3309
EcoT14I	CCWWGG	3 cut(s)	385, 2244, 2761
EcoT22I	ATGCAT	2 cut(s)	410, 882
EcoT38I	GRGCYC	3 cut(s)	197, 1167, 2335
ErhI	CCWWGG	3 cut(s)	385, 2244, 2761
Esp3I	CGTCTC	1 cut(s)	1256
FaqI	GGGAC	3 cut(s)	151, 869, 1432
FauI	CCCGC	2 cut(s)	1586, 2379
FauNDI	CATATG	2 cut(s)	476, 2949
FblI	GTMKAC	3 cut(s)	552, 1305, 1431
Fnu4HI	GCNGC	7 cut(s)	677, 954, 1292, 1488, 1509, 1844, 3407
FriOI	GRGCYC	3 cut(s)	197, 1167, 2335
Fsp4HI	GCNGC	7 cut(s)	677, 954, 1292, 1488, 1509, 1844, 3407
GlaI	GCGC	3 cut(s)	174, 1474, 1476
GluI	GCNGC	7 cut(s)	677, 954, 1292, 1488, 1509, 1844, 3407
GsaI	CCCAGC	3 cut(s)	1722, 1986, 3233
GsuI	CTGGAG	4 cut(s)	53, 774, 978, 1396
HaeII	RGCGCY	1 cut(s)	176
HaeIII	GGCC	9 cut(s)	1071, 1503, 1846, 2223, 2249, 2839, 2910, 2993, 3275
HapII	CCGG	6 cut(s)	1514, 1532, 1540, 2216, 2726, 3324
HgaI	GACGC	3 cut(s)	976, 1442, 1908
HhaI	GCGC	3 cut(s)	175, 1475, 1477
Hin1I	GRCGYC	1 cut(s)	1900
Hin6I	GCGC	3 cut(s)	173, 1473, 1475
HinP1I	GCGC	3 cut(s)	173, 1473, 1475
HincII	GTYRAC	1 cut(s)	2191
HindII	GTYRAC	1 cut(s)	2191
HindIII	AAGCTT	5 cut(s)	202, 1081, 2514, 2963, 3143
HpaI	GTTAAC	1 cut(s)	2191
HpaII	CCGG	6 cut(s)	1514, 1532, 1540, 2216, 2726, 3324
HphI	GGTGA	9 cut(s)	697, 803, 966, 1091, 1636, 2723, 2795, 3272, 3356
HpyAV	CCTTC	9 cut(s)	312, 1039, 1900, 2053, 2213, 2476, 2658, 2850, 3416
HpyCH4IV	ACGT	4 cut(s)	155, 2063, 2345, 2602
HpyF3I	CTNAG	8 cut(s)	200, 324, 579, 1662, 2304, 2334, 2694, 2988
HpySE526I	ACGT	4 cut(s)	155, 2063, 2345, 2602
Hsp92I	GRCGYC	1 cut(s)	1900
HspAI	GCGC	3 cut(s)	173, 1473, 1475
Kpn2I	TCCGGA	2 cut(s)	2215, 2725
KspAI	GTTAAC	1 cut(s)	2191
Kzo9I	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
LguI	GCTCTTC	4 cut(s)	842, 993, 2421, 2442
Lsp1109I	GCAGC	3 cut(s)	663, 965, 3418
MaeII	ACGT	4 cut(s)	155, 2063, 2345, 2602
MalI	GATC	8 cut(s)	182, 391, 1566, 1703, 1948, 2411, 2547, 2926
MbiI	CCGCTC	1 cut(s)	1511
MboI	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
MfeI	CAATTG	3 cut(s)	1020, 2082, 3066
MflI	RGATCY	2 cut(s)	2409, 2924
MhlI	GDGCHC	6 cut(s)	100, 197, 897, 1005, 1167, 2335
MlsI	TGGCCA	1 cut(s)	2249
MluNI	TGGCCA	1 cut(s)	2249
MlyI	GAGTC	6 cut(s)	502, 673, 1311, 1425, 1933, 2825
MmeI	TCCRAC	5 cut(s)	678, 1426, 2003, 2401, 2758
Mox20I	TGGCCA	1 cut(s)	2249
Mph1103I	ATGCAT	2 cut(s)	410, 882
MroI	TCCGGA	2 cut(s)	2215, 2725
MroXI	GAANNNNTTC	1 cut(s)	2001
MscI	TGGCCA	1 cut(s)	2249
MslI	CAYNNNNRTG	3 cut(s)	1815, 3042, 3387
Msp20I	TGGCCA	1 cut(s)	2249
MspA1I	CMGCKG	4 cut(s)	956, 1294, 1982, 2987
MspI	CCGG	6 cut(s)	1514, 1532, 1540, 2216, 2726, 3324
MspR9I	CCNGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
MunI	CAATTG	3 cut(s)	1020, 2082, 3066
Mva1269I	GAATGC	2 cut(s)	880, 1241
MvaI	CCWGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
MvnI	CGCG	2 cut(s)	1475, 1490
NcoI	CCATGG	1 cut(s)	2244
NdeI	CATATG	2 cut(s)	476, 2949
NdeII	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
NlaIV	GGNNCC	7 cut(s)	36, 167, 750, 1002, 2411, 2598, 2955
NmeAIII	GCCGAG	2 cut(s)	1449, 1479
NmuCI	GTSAC	8 cut(s)	685, 791, 940, 972, 1642, 3073, 3260, 3352
NsiI	ATGCAT	2 cut(s)	410, 882
NspI	RCATGY	2 cut(s)	3029, 3435
NspV	TTCGAA	1 cut(s)	2843
PagI	TCATGA	4 cut(s)	774, 1143, 2272, 3120
PaqCI	CACCTGC	1 cut(s)	1103
PauI	GCGCGC	1 cut(s)	1473
PceI	AGGCCT	1 cut(s)	2839
PciI	ACATGT	1 cut(s)	3025
PciSI	GCTCTTC	4 cut(s)	842, 993, 2421, 2442
PcsI	WCGNNNNNNNCGW	2 cut(s)	1383, 1425
PctI	GAATGC	2 cut(s)	880, 1241
PdmI	GAANNNNTTC	1 cut(s)	2001
PfeI	GAWTC	8 cut(s)	16, 1196, 1315, 1625, 2015, 2308, 2938, 3173
PflFI	GACNNNGTC	2 cut(s)	970, 1436
PflMI	CCANNNNNTGG	1 cut(s)	2606
PfoI	TCCNGGA	1 cut(s)	751
PkrI	GCNGC	7 cut(s)	678, 955, 1293, 1489, 1510, 1845, 3408
PleI	GAGTC	6 cut(s)	501, 672, 1310, 1424, 1933, 2825
PmaCI	CACGTG	1 cut(s)	2603
PmlI	CACGTG	1 cut(s)	2603
PpsI	GAGTC	6 cut(s)	501, 672, 1310, 1424, 1933, 2825
Ppu21I	YACGTR	1 cut(s)	2603
PpuMI	RGGWCCY	1 cut(s)	165
PscI	ACATGT	1 cut(s)	3025
PshBI	ATTAAT	1 cut(s)	1824
PsiI	TTATAA	2 cut(s)	2720, 3096
Psp124BI	GAGCTC	3 cut(s)	197, 1167, 2335
Psp5II	RGGWCCY	1 cut(s)	165
Psp6I	CCWGG	6 cut(s)	670, 725, 751, 919, 1646, 3309
PspCI	CACGTG	1 cut(s)	2603
PspEI	GGTNACC	1 cut(s)	2392
PspFI	CCCAGC	3 cut(s)	1718, 1982, 3229
PspGI	CCWGG	6 cut(s)	670, 725, 751, 919, 1646, 3309
PspN4I	GGNNCC	7 cut(s)	36, 167, 750, 1002, 2411, 2598, 2955
PspPI	GGNCC	7 cut(s)	165, 1016, 1070, 1213, 2222, 2908, 3425
PspPPI	RGGWCCY	1 cut(s)	165
PstNI	CAGNNNCTG	1 cut(s)	1193
PsuI	RGATCY	2 cut(s)	2409, 2924
PsyI	GACNNNGTC	2 cut(s)	970, 1436
PteI	GCGCGC	1 cut(s)	1473
PvuII	CAGCTG	2 cut(s)	956, 2987
RsaI	GTAC	7 cut(s)	415, 763, 1562, 1571, 1738, 2871, 3102
RsaNI	GTAC	7 cut(s)	414, 762, 1561, 1570, 1737, 2870, 3101
RseI	CAYNNNNRTG	3 cut(s)	1815, 3042, 3387
SacI	GAGCTC	3 cut(s)	197, 1167, 2335
SapI	GCTCTTC	4 cut(s)	842, 993, 2421, 2442
SatI	GCNGC	7 cut(s)	677, 954, 1292, 1488, 1509, 1844, 3407
Sau3AI	GATC	8 cut(s)	180, 389, 1564, 1701, 1946, 2409, 2545, 2924
Sau96I	GGNCC	7 cut(s)	165, 1016, 1070, 1213, 2222, 2908, 3425
SchI	GAGTC	6 cut(s)	502, 673, 1311, 1425, 1933, 2825
ScrFI	CCNGG	6 cut(s)	672, 727, 753, 921, 1648, 3311
SduI	GDGCHC	6 cut(s)	100, 197, 897, 1005, 1167, 2335
SfuI	TTCGAA	1 cut(s)	2843
SinI	GGWCC	4 cut(s)	165, 1016, 1213, 3425
SmiMI	CAYNNNNRTG	3 cut(s)	1815, 3042, 3387
SmlI	CTYRAG	3 cut(s)	1950, 2105, 2833
SmoI	CTYRAG	3 cut(s)	1950, 2105, 2833
SpeI	ACTAGT	1 cut(s)	2654
SseBI	AGGCCT	1 cut(s)	2839
SsiI	CCGC	9 cut(s)	1292, 1299, 1488, 1509, 1579, 1843, 1980, 2372, 3393
SspI	AATATT	1 cut(s)	2010
SstI	GAGCTC	3 cut(s)	197, 1167, 2335
StuI	AGGCCT	1 cut(s)	2839
StyD4I	CCNGG	6 cut(s)	670, 725, 751, 919, 1646, 3309
StyI	CCWWGG	3 cut(s)	385, 2244, 2761
TaiI	ACGT	4 cut(s)	158, 2066, 2348, 2605
TaqII	GACCGA	1 cut(s)	773
TauI	GCSGC	4 cut(s)	1294, 1490, 1511, 1846
TfiI	GAWTC	8 cut(s)	16, 1196, 1315, 1625, 2015, 2308, 2938, 3173
TscAI	CASTG	7 cut(s)	2556, 2598, 2880, 2892, 3001, 3020, 3310
TseFI	GTSAC	8 cut(s)	685, 791, 940, 972, 1642, 3073, 3260, 3352
TseI	GCWGC	3 cut(s)	676, 953, 3406
Tsp45I	GTSAC	8 cut(s)	685, 791, 940, 972, 1642, 3073, 3260, 3352
TspDTI	ATGAA	7 cut(s)	763, 2457, 2697, 2763, 2771, 2966, 3171
TspGWI	ACGGA	1 cut(s)	491
TspRI	CASTG	7 cut(s)	2556, 2598, 2880, 2892, 3001, 3020, 3310
Tth111I	GACNNNGTC	2 cut(s)	970, 1436
Van91I	CCANNNNNTGG	1 cut(s)	2606
VpaK11BI	GGWCC	4 cut(s)	165, 1016, 1213, 3425
VspI	ATTAAT	1 cut(s)	1824
XapI	RAATTY	3 cut(s)	865, 1916, 3317
XceI	RCATGY	2 cut(s)	3029, 3435
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2251
XmiI	GTMKAC	3 cut(s)	552, 1305, 1431
XmnI	GAANNNNTTC	1 cut(s)	2001
Zsp2I	ATGCAT	2 cut(s)	410, 882

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.