Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0006213

Physical Location & Seq

Genomic Coordinates Reverse (-)

65594922 .. 65601453

Gene Structure 6532 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG428300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2385 bp

ATGGGAACTTCGGTTCAGGTAACGCCGCTGTGCGGAGTCTACAACGAGAATCCACTCTCCTATTTGGTCTCCATCGACGGCTTCAACTTCCTCATCGACTGTGGCTGGAACGACCACTTCGACCCCTCCCTCCTCCAACCTCTCTCCAGAGTCGCTTCCACCGTAGACGCCGTCCTACTGTCCCACCCCGACACACTCCACCTCGGCGCGCTTCCCTACGCCGCGAAACACCTCGGCCTCGCCGCTCCGGTCTTCTCCACCGAGCCGGTTTACCGGCTAGGGCTTCTCACTATGTACGATCAGTACCTCTCCCGCAAGCAAGTTTCAGAGTTCGATTTGTTTACGCTGGATGATATTGATTCTGCTTTCCAAAATGTCACCAGGCTGACCAATGCTCAGCACCATCATCTTACTGGCAAAGGAGAGGGAATTGTGATCTCGCCTCATGTGGCTGGGCATCTATTGGGAGGTACTGTGTGGAAGATAACAAAGGATGGAGAGGATGTTATATATGCTGTGGACTTCAACCATCGAAAAGAAAAGCATTTGAATGGAATTAATCAGTCATCGTTTGTGCGGCCTGCTGTTCTAATAACAGATGCCTATAATGCTTTGAACAATCAGCCTTATAGACGCCAGAAGGACAGAGAATTTACAGAGACTATAAAGAAGACTCTGCGATCTCAAGGAAATGTTTTACTACCTGTTGATACTGCTGGGCGAGTTTTGGAACTTCTTCAAATATTGGAATCGTGTTGGAACGAGGAAAGTTTGCCCTTCCCCATCTATTTCCTAACGTATGTTGCTTCTAGCACAATTGATTATGTCAAAAGTTTCCTTGAGTGGATGAGTGATGCAATGGCAAAGTCCTTTGAGACAACACGAGACAATGCTTTTATTTTGAAGCGTGTGAAACTTCTTGTTAACAAGAGCGAACTTGACAATGCTCCGGAAGGCCCAAAGGTATACCATATATACTTTGTGAAGTATTTCCTTATGATGCCTACCAGATTGAATGATGTTGACATGTGCATTCCCATACAGGTTGTTTTAGCATCCATGGCCAGTTTGGAAGCGGGTTTTTCTCACGATATATTTGTCGAATGGGCAACTGATGCTAAGAATCTTGTGCTTTTTACAGAACGAGCTCAGTTTGGCACGTTAGCTCGTATGCTTCAGGCAGACCCGCCTCCAAAAGCTGTCAAGGTTACCATGTCCAAGAGGATCCCTTTGGTTGGAGAAGAGCTAATTGCTTATGAAGAAGAGCAGAACAGAATTAAAAAGGAAGAAGCTCTAAAGGCTAGTCTGATTAAAGAGGAGGAGTTAAAAGCTTCTCATGGGGCTGATGTCAGCATGAGTGATCCACTGGTTATTGATACTAGCAATGCAAAGTCTTTGCCAGATGTGGTTCCACGTGCCGGTGGATGCAGAGACATTTTAATCGATGGATTTACTCCTCCTTCTACTAGTGTTGCCCCTATGTTTCCCTTCTATGAAAATAATTCTGAGTGGGATGACTTTGGTGAAGTTATAAATCCGGATGATTATGTTATCAAGGATGAAGACATGAACCAAGGAGCAATGCTTGTTGGAGGTGATATGGATGGAAAAATTGACGAAGCCTCTGCTAGTTTAATACTCGACTCAAGGCCTTCGAAAGTTGTGTCAAGTGAATTGACTGTACCAGTGAAGTGTTCACTGATATATATGGACTTTGAGGGCCGTTCTGATGCGAGATCTGTTAAATCGATTCTTTCGCATATGGCTCCTCTAAAGCTTGTTTTGGTTCATGGAACAGCTGAGGCCACTGAACATTTGAAGCAACACTGCTTGAAACATGTTTGCCCTCATGTATATGCTCCTCAACTTGAAGAAACAATTGATGTCACATCTGATTTATGTGCTTATAAGGTAATCTCAGAATTTCTTTTTGTTTTGTTGATCTGTGATTCCGAAATCACAATTTTCTCCATTTCCCATGGTGTTGTAATGCAGGTACAACTGTCCGAGAAGCTCATGAGTAACATAATCTTTAAGAAGCTTGGAGAGAATGAAATAGCTTGGTTCGATTCTGATGTAGGGAAGACAGAAGACGAAATGCTGACTTTACTACCCTGCTCAACCCCAGCTCCACCACACAAACCAATCCTAGTTGGTGACTTAAAAATGGCCGATTTCAAGCAATATCTTGCTGATAACGGTGTCCAGGTTGAATTTGCCAGTGGGGCTTTGCGATGTGGTGAATATGTGACAATACGCAAGGTTGGGGATGCAAGCCATAAGGGTGGCGGTGCTGGTTCGCAGCAAATTGTGATTGAAGGTCCAGCATGTGAGGATTATTACAAGATACGAGAATATCTCTATTCACACTTTTATTTACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005847 GO:0005849 GO:0005911 GO:0006139 GO:0006378 GO:0006379 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0030054 GO:0031047 GO:0031123 GO:0031124 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0055044 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0090304 GO:0090305 GO:0090501 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03015 map03015

Pfam Domains

Protein Families

Beta-Casp CPSF100_C Lactamase_B_6 RMMBL

Protein Analysis

794

Amino Acids

88.45

Weight (kDa)

5.11

Isoelectric Point (pI)

38.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lactamase_B	PF00753	19 - 149	2.1e-07	Metallo-beta-lactamase superfamily
Lactamase_B_6	PF16661	22 - 195	2.2e-53	Metallo-beta-lactamase superfamily domain
Beta-Casp	PF10996	242 - 392	2.1e-18	Beta-Casp domain
RMMBL	PF07521	561 - 611	3.2e-10	Zn-dependent metallo-hydrolase RNA specificity domain
CPSF100_C	PF13299	699 - 791	4.9e-19	Cleavage and polyadenylation factor 2 C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0006213)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23880
fragaria_vesca	FvH4_5g14450 FvH4_7g29660 FvH4_7g29660
malus_domestica	MD01G1203600.v1.1 MD07G1272900.v1.1
prunus_persica	Prupe.2G296200_v2.0.a1 Prupe.2G296200_v2.0.a1
pyrus_communis	pycom01g21270 pycom01g21280 pycom07g24930
rosa_chinensis	RchiOBHm_Chr1g0377671 RchiOBHm_Chr1g0377681 RchiOBHm_Chr1g0379161 RchiOBHm_Chr1g0379331
rosa_laevigata	RLG00000026506 RLG00000026773
rosa_multiflora	Rmu_sc0002832.1_g000015
rosa_roxburghii	Rroxscaffold_4G00281130
rosa_rugosa	Rorug01G0404200 Rorug01G0404300
rosa_samantha	Rh1AG428300 Rh1BG381000 Rh1CG000300 Rh1CG395000 Rh1DG412000
rosa_wichuraiana	Rw1G037040

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1532, 1908
Acc36I	ACCTGC	1 cut(s)	1984
AccBSI	CCGCTC	1 cut(s)	245
AccI	GTMKAC	3 cut(s)	39, 165, 966
AccII	CGCG	2 cut(s)	209, 224
AccIII	TCCGGA	2 cut(s)	949, 1537
AciI	CCGC	9 cut(s)	26, 33, 222, 243, 313, 577, 1076, 1187, 2289
AclWI	GGATC	3 cut(s)	1219, 1232, 1355
AcoI	YGGCCR	2 cut(s)	1062, 2169
AcsI	RAATTY	3 cut(s)	650, 1922, 2213
AcuI	CTGAAG	1 cut(s)	1160
AcvI	CACGTG	1 cut(s)	1415
AcyI	GRCGYC	2 cut(s)	168, 634
AfaI	GTAC	5 cut(s)	296, 305, 472, 1683, 1998
AfiI	CCNNNNNNNGG	3 cut(s)	32, 1235, 1418
AflIII	ACRYGT	2 cut(s)	1026, 1837
AhlI	ACTAGT	1 cut(s)	1466
AjnI	CCWGG	2 cut(s)	380, 2205
AloI	GAACNNNNNNTCC	1 cut(s)	29
Alw21I	GWGCWC	1 cut(s)	1150
Alw26I	GTCTC	5 cut(s)	73, 653, 869, 879, 1425
AlwI	GGATC	3 cut(s)	1219, 1232, 1355
Aor13HI	TCCGGA	2 cut(s)	949, 1537
AoxI	GGCC	8 cut(s)	235, 578, 955, 1062, 1649, 1720, 1803, 2169
ApeKI	GCWGC	1 cut(s)	2302
ApoI	RAATTY	3 cut(s)	650, 1922, 2213
ArsI	GACNNNNNNTTYG	2 cut(s)	1649, 1681
AseI	ATTAAT	1 cut(s)	558
Asp700I	GAANNNNTTC	2 cut(s)	735, 989
AspLEI	GCGC	2 cut(s)	209, 211
AspS9I	GGNCC	3 cut(s)	956, 1720, 2321
AsuHPI	GGTGA	5 cut(s)	370, 1535, 1607, 2168, 2252
AsuII	TTCGAA	1 cut(s)	1655
AvaII	GGWCC	1 cut(s)	2321
BaeI	ACNNNNGTAYC	2 cut(s)	1988, 2021
BalI	TGGCCA	1 cut(s)	1064
BamHI	GGATCC	1 cut(s)	1224
BanII	GRGCYC	1 cut(s)	1150
BauI	CACGAG	1 cut(s)	882
BbrPI	CACGTG	1 cut(s)	1415
BbsI	GAAGAC	5 cut(s)	244, 677, 1569, 2090, 2097
Bbv12I	GWGCWC	1 cut(s)	1150
BbvCI	CCTCAGC	1 cut(s)	1800
BbvI	GCAGC	1 cut(s)	2314
BccI	CCATC	7 cut(s)	80, 411, 488, 537, 791, 1439, 1598
BceAI	ACGGC	3 cut(s)	94, 155, 1707
BcgI	CGANNNNNNTGC	2 cut(s)	1607, 1641
BciT130I	CCWGG	2 cut(s)	382, 2207
BcoDI	GTCTC	5 cut(s)	73, 653, 869, 879, 1425
BcuI	ACTAGT	1 cut(s)	1466
BfaI	CTAG	7 cut(s)	278, 810, 1302, 1380, 1467, 1629, 2150
BfuAI	ACCTGC	1 cut(s)	1984
BglI	GCCNNNNNGGC	1 cut(s)	2225
BglII	AGATCT	1 cut(s)	1736
BisI	GCNGC	5 cut(s)	26, 222, 243, 578, 2303
BlpI	GCTNAGC	1 cut(s)	396
BlsI	GCNGC	5 cut(s)	27, 223, 244, 579, 2304
Bme1390I	CCNGG	2 cut(s)	382, 2207
Bme18I	GGWCC	1 cut(s)	2321
BmgT120I	GGNCC	3 cut(s)	956, 1720, 2321
BmiI	GGNNCC	3 cut(s)	1226, 1410, 1767
BmrFI	CCNGG	2 cut(s)	382, 2207
BmsI	GCATC	9 cut(s)	466, 589, 844, 990, 1064, 1105, 1415, 1720, 2260
BpiI	GAAGAC	5 cut(s)	244, 677, 1569, 2090, 2097
BplI	GAGNNNNNCTC	2 cut(s)	2343, 2375
BpmI	CTGGAG	1 cut(s)	130
Bpu10I	CCTNAGC	1 cut(s)	1800
Bpu1102I	GCTNAGC	1 cut(s)	396
Bpu14I	TTCGAA	1 cut(s)	1655
BpuEI	CTTGAG	3 cut(s)	669, 860, 1630
Bsa29I	ATCGAT	2 cut(s)	1443, 1748
BsaAI	YACGTR	1 cut(s)	1415
BsaHI	GRCGYC	2 cut(s)	168, 634
BsaI	GGTCTC	1 cut(s)	73
BsaJI	CCNNGG	5 cut(s)	202, 232, 1059, 1573, 1978
BsaWI	WCCGGW	3 cut(s)	247, 949, 1537
BsaXI	ACNNNNNCTCC	6 cut(s)	459, 489, 489, 519, 2113, 2143
Bsc4I	CCNNNNNNNGG	3 cut(s)	32, 1235, 1418
Bse118I	RCCGGY	3 cut(s)	265, 273, 1418
Bse1I	ACTGG	5 cut(s)	418, 1065, 1371, 1685, 2220
Bse3DI	GCAATG	3 cut(s)	864, 1390, 1587
BseAI	TCCGGA	2 cut(s)	949, 1537
BseBI	CCWGG	2 cut(s)	382, 2207
BseCI	ATCGAT	2 cut(s)	1443, 1748
BseDI	CCNNGG	5 cut(s)	202, 232, 1059, 1573, 1978
BseLI	CCNNNNNNNGG	3 cut(s)	32, 1235, 1418
BseMI	GCAATG	3 cut(s)	864, 1390, 1587
BseMII	CTCAG	5 cut(s)	410, 1163, 1497, 1791, 1932
BseNI	ACTGG	5 cut(s)	418, 1065, 1371, 1685, 2220
BsePI	GCGCGC	1 cut(s)	207
BseRI	GAGGAG	6 cut(s)	122, 1331, 1334, 1446, 1758, 1851
BseXI	GCAGC	1 cut(s)	2314
BseYI	CCCAGC	3 cut(s)	452, 716, 2125
Bsh1236I	CGCG	2 cut(s)	209, 224
BshFI	GGCC	8 cut(s)	237, 580, 957, 1064, 1651, 1722, 1805, 2171
BshVI	ATCGAT	2 cut(s)	1443, 1748
BsiHKAI	GWGCWC	1 cut(s)	1150
BsiSI	CCGG	6 cut(s)	248, 266, 274, 950, 1419, 1538
BslFI	GGGAC	1 cut(s)	166
BslI	CCNNNNNNNGG	3 cut(s)	32, 1235, 1418
BsmAI	GTCTC	5 cut(s)	73, 653, 869, 879, 1425
BsmFI	GGGAC	1 cut(s)	166
BsmI	GAATGC	1 cut(s)	1032
BsnI	GGCC	8 cut(s)	237, 580, 957, 1064, 1651, 1722, 1805, 2171
Bso31I	GGTCTC	1 cut(s)	73
Bsp119I	TTCGAA	1 cut(s)	1655
Bsp1286I	GDGCHC	1 cut(s)	1150
Bsp13I	TCCGGA	2 cut(s)	949, 1537
Bsp143I	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
Bsp1720I	GCTNAGC	1 cut(s)	396
Bsp19I	CCATGG	2 cut(s)	1059, 1978
BspACI	CCGC	9 cut(s)	26, 33, 222, 243, 313, 577, 1076, 1187, 2289
BspANI	GGCC	8 cut(s)	237, 580, 957, 1064, 1651, 1722, 1805, 2171
BspCNI	CTCAG	5 cut(s)	409, 1162, 1498, 1792, 1931
BspDI	ATCGAT	2 cut(s)	1443, 1748
BspEI	TCCGGA	2 cut(s)	949, 1537
BspFNI	CGCG	2 cut(s)	209, 224
BspHI	TCATGA	1 cut(s)	2016
BspLI	GGNNCC	3 cut(s)	1226, 1410, 1767
BspMI	ACCTGC	1 cut(s)	1984
BspPI	GGATC	3 cut(s)	1219, 1232, 1355
BspQI	GCTCTTC	2 cut(s)	1236, 1257
BspT104I	TTCGAA	1 cut(s)	1655
BspTNI	GGTCTC	1 cut(s)	73
BsrBI	CCGCTC	1 cut(s)	245
BsrDI	GCAATG	3 cut(s)	864, 1390, 1587
BsrFI	RCCGGY	3 cut(s)	265, 273, 1418
BsrI	ACTGG	5 cut(s)	418, 1065, 1371, 1685, 2220
BssAI	RCCGGY	3 cut(s)	265, 273, 1418
BssECI	CCNNGG	5 cut(s)	202, 232, 1059, 1573, 1978
BssHII	GCGCGC	1 cut(s)	207
BssMI	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
BssNAI	GTATAC	1 cut(s)	967
BssNI	GRCGYC	2 cut(s)	168, 634
BssSI	CACGAG	1 cut(s)	882
BssT1I	CCWWGG	3 cut(s)	1059, 1573, 1978
Bst1107I	GTATAC	1 cut(s)	967
Bst2BI	CACGAG	1 cut(s)	882
Bst2UI	CCWGG	2 cut(s)	382, 2207
Bst4CI	ACNGT	7 cut(s)	101, 163, 180, 475, 1681, 2004, 2201
Bst6I	CTCTTC	2 cut(s)	1236, 1257
BstACI	GRCGYC	2 cut(s)	168, 634
BstAPI	GCANNNNNTGC	1 cut(s)	1115
BstBAI	YACGTR	1 cut(s)	1415
BstBI	TTCGAA	1 cut(s)	1655
BstC8I	GCNNGC	4 cut(s)	209, 317, 582, 2275
BstDEI	CTNAG	6 cut(s)	396, 1119, 1149, 1506, 1800, 1918
BstDSI	CCRYGG	2 cut(s)	1059, 1978
BstEII	GGTNACC	1 cut(s)	1207
BstFNI	CGCG	2 cut(s)	209, 224
BstHHI	GCGC	2 cut(s)	209, 211
BstKTI	GATC	7 cut(s)	301, 438, 683, 1227, 1363, 1739, 1944
BstMAI	GTCTC	5 cut(s)	73, 653, 869, 879, 1425
BstMBI	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
BstMWI	GCNNNNNNNGC	5 cut(s)	608, 1061, 1115, 1298, 2225
BstNI	CCWGG	2 cut(s)	382, 2207
BstNSI	RCATGY	3 cut(s)	1030, 1841, 2331
BstPI	GGTNACC	1 cut(s)	1207
BstSCI	CCNGG	2 cut(s)	380, 2205
BstUI	CGCG	2 cut(s)	209, 224
BstV1I	GCAGC	1 cut(s)	2314
BstV2I	GAAGAC	5 cut(s)	244, 677, 1569, 2090, 2097
BstX2I	RGATCY	2 cut(s)	1224, 1736
BstXI	CCANNNNNNTGG	1 cut(s)	2285
BstYI	RGATCY	2 cut(s)	1224, 1736
BstZ17I	GTATAC	1 cut(s)	967
Bsu15I	ATCGAT	2 cut(s)	1443, 1748
BsuRI	GGCC	8 cut(s)	237, 580, 957, 1064, 1651, 1722, 1805, 2171
BsuTUI	ATCGAT	2 cut(s)	1443, 1748
BtgI	CCRYGG	2 cut(s)	1059, 1978
BtgZI	GCGATG	1 cut(s)	2248
BtsI	GCAGTG	1 cut(s)	1825
BtsIMutI	CAGTG	6 cut(s)	1364, 1692, 1697, 1806, 1825, 2227
BveI	ACCTGC	1 cut(s)	1984
Cac8I	GCNNGC	4 cut(s)	209, 317, 582, 2275
CciI	TCATGA	1 cut(s)	2016
CfoI	GCGC	2 cut(s)	209, 211
Cfr10I	RCCGGY	3 cut(s)	265, 273, 1418
Cfr13I	GGNCC	3 cut(s)	956, 1720, 2321
ClaI	ATCGAT	2 cut(s)	1443, 1748
CseI	GACGC	2 cut(s)	176, 642
Csp6I	GTAC	5 cut(s)	295, 304, 471, 1682, 1997
CviQI	GTAC	5 cut(s)	295, 304, 471, 1682, 1997
DdeI	CTNAG	6 cut(s)	396, 1119, 1149, 1506, 1800, 1918
DpnI	GATC	7 cut(s)	300, 437, 682, 1226, 1362, 1738, 1943
DpnII	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
EaeI	YGGCCR	2 cut(s)	1062, 2169
Eam1104I	CTCTTC	2 cut(s)	1236, 1257
EarI	CTCTTC	2 cut(s)	1236, 1257
Ecl136II	GAGCTC	1 cut(s)	1148
Eco130I	CCWWGG	3 cut(s)	1059, 1573, 1978
Eco147I	AGGCCT	1 cut(s)	1651
Eco24I	GRGCYC	1 cut(s)	1150
Eco31I	GGTCTC	1 cut(s)	73
Eco47I	GGWCC	1 cut(s)	2321
Eco53kI	GAGCTC	1 cut(s)	1148
Eco57I	CTGAAG	1 cut(s)	1160
Eco72I	CACGTG	1 cut(s)	1415
Eco91I	GGTNACC	1 cut(s)	1207
EcoICRI	GAGCTC	1 cut(s)	1148
EcoO65I	GGTNACC	1 cut(s)	1207
EcoRII	CCWGG	2 cut(s)	380, 2205
EcoT14I	CCWWGG	3 cut(s)	1059, 1573, 1978
EcoT38I	GRGCYC	1 cut(s)	1150
ErhI	CCWWGG	3 cut(s)	1059, 1573, 1978
FaqI	GGGAC	1 cut(s)	166
FauI	CCCGC	3 cut(s)	320, 1069, 1194
FauNDI	CATATG	1 cut(s)	1761
FblI	GTMKAC	3 cut(s)	39, 165, 966
Fnu4HI	GCNGC	5 cut(s)	26, 222, 243, 578, 2303
FriOI	GRGCYC	1 cut(s)	1150
Fsp4HI	GCNGC	5 cut(s)	26, 222, 243, 578, 2303
FspBI	CTAG	7 cut(s)	278, 810, 1302, 1380, 1467, 1629, 2150
GlaI	GCGC	2 cut(s)	208, 210
GluI	GCNGC	5 cut(s)	26, 222, 243, 578, 2303
GsaI	CCCAGC	3 cut(s)	456, 720, 2129
GsuI	CTGGAG	1 cut(s)	130
HaeIII	GGCC	8 cut(s)	237, 580, 957, 1064, 1651, 1722, 1805, 2171
HapII	CCGG	6 cut(s)	248, 266, 274, 950, 1419, 1538
HgaI	GACGC	2 cut(s)	176, 642
HhaI	GCGC	2 cut(s)	209, 211
Hin1I	GRCGYC	2 cut(s)	168, 634
Hin6I	GCGC	2 cut(s)	207, 209
HinP1I	GCGC	2 cut(s)	207, 209
HincII	GTYRAC	2 cut(s)	925, 1024
HindII	GTYRAC	2 cut(s)	925, 1024
HindIII	AAGCTT	3 cut(s)	1329, 1775, 2039
HpaI	GTTAAC	1 cut(s)	925
HpaII	CCGG	6 cut(s)	248, 266, 274, 950, 1419, 1538
HphI	GGTGA	5 cut(s)	370, 1535, 1607, 2168, 2252
Hpy166II	GTNNAC	9 cut(s)	40, 166, 271, 342, 520, 925, 967, 1024, 1697
Hpy188I	TCNGA	9 cut(s)	328, 1308, 1507, 1729, 1894, 1921, 1954, 2008, 2074
Hpy188III	TCNNGA	5 cut(s)	147, 950, 1088, 1538, 2017
Hpy8I	GTNNAC	9 cut(s)	40, 166, 271, 342, 520, 925, 967, 1024, 1697
Hpy99I	CGWCG	1 cut(s)	80
HpyAV	CCTTC	7 cut(s)	634, 787, 947, 1470, 1498, 1662, 2312
HpyCH4III	ACNGT	7 cut(s)	101, 163, 180, 475, 1681, 2004, 2201
HpyCH4IV	ACGT	3 cut(s)	797, 1160, 1414
HpyCH4V	TGCA	6 cut(s)	857, 1032, 1388, 1428, 1993, 2273
HpyF10VI	GCNNNNNNNGC	5 cut(s)	608, 1061, 1115, 1298, 2225
HpyF3I	CTNAG	6 cut(s)	396, 1119, 1149, 1506, 1800, 1918
HpySE526I	ACGT	3 cut(s)	797, 1160, 1414
Hsp92I	GRCGYC	2 cut(s)	168, 634
HspAI	GCGC	2 cut(s)	207, 209
Kpn2I	TCCGGA	2 cut(s)	949, 1537
KspAI	GTTAAC	1 cut(s)	925
Kzo9I	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
LguI	GCTCTTC	2 cut(s)	1236, 1257
LmnI	GCTCC	6 cut(s)	250, 952, 1577, 1771, 1864, 2134
Lsp1109I	GCAGC	1 cut(s)	2314
LweI	GCATC	9 cut(s)	466, 589, 844, 990, 1064, 1105, 1415, 1720, 2260
MaeI	CTAG	7 cut(s)	278, 810, 1302, 1380, 1467, 1629, 2150
MaeII	ACGT	3 cut(s)	797, 1160, 1414
MaeIII	GTNAC	7 cut(s)	19, 376, 1207, 1885, 2021, 2156, 2248
MalI	GATC	7 cut(s)	300, 437, 682, 1226, 1362, 1738, 1943
MbiI	CCGCTC	1 cut(s)	245
MboI	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
MfeI	CAATTG	2 cut(s)	816, 1878
MflI	RGATCY	2 cut(s)	1224, 1736
MhlI	GDGCHC	1 cut(s)	1150
MlsI	TGGCCA	1 cut(s)	1064
MluNI	TGGCCA	1 cut(s)	1064
MlyI	GAGTC	4 cut(s)	45, 159, 667, 1637
MmeI	TCCRAC	4 cut(s)	160, 737, 1216, 1570
Mox20I	TGGCCA	1 cut(s)	1064
MroI	TCCGGA	2 cut(s)	949, 1537
MroXI	GAANNNNTTC	2 cut(s)	735, 989
MscI	TGGCCA	1 cut(s)	1064
MslI	CAYNNNNRTG	3 cut(s)	549, 1854, 2283
Msp20I	TGGCCA	1 cut(s)	1064
MspA1I	CMGCKG	2 cut(s)	28, 1799
MspI	CCGG	6 cut(s)	248, 266, 274, 950, 1419, 1538
MspR9I	CCNGG	2 cut(s)	382, 2207
MunI	CAATTG	2 cut(s)	816, 1878
Mva1269I	GAATGC	1 cut(s)	1032
MvaI	CCWGG	2 cut(s)	382, 2207
MvnI	CGCG	2 cut(s)	209, 224
MwoI	GCNNNNNNNGC	5 cut(s)	608, 1061, 1115, 1298, 2225
NcoI	CCATGG	2 cut(s)	1059, 1978
NdeI	CATATG	1 cut(s)	1761
NdeII	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
NlaIV	GGNNCC	3 cut(s)	1226, 1410, 1767
NmeAIII	GCCGAG	2 cut(s)	183, 213
NmuCI	GTSAC	4 cut(s)	376, 1885, 2156, 2248
NspI	RCATGY	3 cut(s)	1030, 1841, 2331
NspV	TTCGAA	1 cut(s)	1655
PagI	TCATGA	1 cut(s)	2016
PauI	GCGCGC	1 cut(s)	207
PceI	AGGCCT	1 cut(s)	1651
PciI	ACATGT	2 cut(s)	1026, 1837
PciSI	GCTCTTC	2 cut(s)	1236, 1257
PcsI	WCGNNNNNNNCGW	2 cut(s)	117, 159
PctI	GAATGC	1 cut(s)	1032
PdmI	GAANNNNTTC	2 cut(s)	735, 989
PfeI	GAWTC	7 cut(s)	49, 359, 749, 1123, 1750, 1949, 2069
PflFI	GACNNNGTC	1 cut(s)	170
PkrI	GCNGC	5 cut(s)	27, 223, 244, 579, 2304
PleI	GAGTC	4 cut(s)	44, 158, 667, 1637
PmaCI	CACGTG	1 cut(s)	1415
PmlI	CACGTG	1 cut(s)	1415
PpsI	GAGTC	4 cut(s)	44, 158, 667, 1637
Ppu21I	YACGTR	1 cut(s)	1415
PscI	ACATGT	2 cut(s)	1026, 1837
PshBI	ATTAAT	1 cut(s)	558
PsiI	TTATAA	2 cut(s)	1532, 1908
Psp124BI	GAGCTC	1 cut(s)	1150
Psp6I	CCWGG	2 cut(s)	380, 2205
PspCI	CACGTG	1 cut(s)	1415
PspEI	GGTNACC	1 cut(s)	1207
PspFI	CCCAGC	3 cut(s)	452, 716, 2125
PspGI	CCWGG	2 cut(s)	380, 2205
PspN4I	GGNNCC	3 cut(s)	1226, 1410, 1767
PspPI	GGNCC	3 cut(s)	956, 1720, 2321
PsuI	RGATCY	2 cut(s)	1224, 1736
PsyI	GACNNNGTC	1 cut(s)	170
PteI	GCGCGC	1 cut(s)	207
PvuII	CAGCTG	1 cut(s)	1799
RsaI	GTAC	5 cut(s)	296, 305, 472, 1683, 1998
RsaNI	GTAC	5 cut(s)	295, 304, 471, 1682, 1997
RseI	CAYNNNNRTG	3 cut(s)	549, 1854, 2283
SacI	GAGCTC	1 cut(s)	1150
SapI	GCTCTTC	2 cut(s)	1236, 1257
SatI	GCNGC	5 cut(s)	26, 222, 243, 578, 2303
Sau3AI	GATC	7 cut(s)	298, 435, 680, 1224, 1360, 1736, 1941
Sau96I	GGNCC	3 cut(s)	956, 1720, 2321
SchI	GAGTC	4 cut(s)	45, 159, 667, 1637
ScrFI	CCNGG	2 cut(s)	382, 2207
SduI	GDGCHC	1 cut(s)	1150
SfaNI	GCATC	9 cut(s)	466, 589, 844, 990, 1064, 1105, 1415, 1720, 2260
SfuI	TTCGAA	1 cut(s)	1655
SinI	GGWCC	1 cut(s)	2321
SmiMI	CAYNNNNRTG	3 cut(s)	549, 1854, 2283
SmlI	CTYRAG	3 cut(s)	684, 839, 1645
SmoI	CTYRAG	3 cut(s)	684, 839, 1645
SpeI	ACTAGT	1 cut(s)	1466
SseBI	AGGCCT	1 cut(s)	1651
SsiI	CCGC	9 cut(s)	26, 33, 222, 243, 313, 577, 1076, 1187, 2289
SspI	AATATT	1 cut(s)	744
SspMI	CTAG	7 cut(s)	278, 810, 1302, 1380, 1467, 1629, 2150
SstI	GAGCTC	1 cut(s)	1150
StuI	AGGCCT	1 cut(s)	1651
StyD4I	CCNGG	2 cut(s)	380, 2205
StyI	CCWWGG	3 cut(s)	1059, 1573, 1978
TaaI	ACNGT	7 cut(s)	101, 163, 180, 475, 1681, 2004, 2201
TaiI	ACGT	3 cut(s)	800, 1163, 1417
TauI	GCSGC	4 cut(s)	28, 224, 245, 580
TfiI	GAWTC	7 cut(s)	49, 359, 749, 1123, 1750, 1949, 2069
TscAI	CASTG	6 cut(s)	1371, 1692, 1704, 1813, 1832, 2227
TseFI	GTSAC	4 cut(s)	376, 1885, 2156, 2248
TseI	GCWGC	1 cut(s)	2302
Tsp45I	GTSAC	4 cut(s)	376, 1885, 2156, 2248
TspDTI	ATGAA	6 cut(s)	1272, 1509, 1575, 1583, 1778, 2067
TspRI	CASTG	6 cut(s)	1371, 1692, 1704, 1813, 1832, 2227
Tth111I	GACNNNGTC	1 cut(s)	170
VpaK11BI	GGWCC	1 cut(s)	2321
VspI	ATTAAT	1 cut(s)	558
XapI	RAATTY	3 cut(s)	650, 1922, 2213
XceI	RCATGY	3 cut(s)	1030, 1841, 2331
XcmI	CCANNNNNNNNNTGG	2 cut(s)	410, 1066
XmiI	GTMKAC	3 cut(s)	39, 165, 966
XmnI	GAANNNNTTC	2 cut(s)	735, 989
XspI	CTAG	7 cut(s)	278, 810, 1302, 1380, 1467, 1629, 2150

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.