Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003545

Physical Location & Seq

Genomic Coordinates Forward (+)

413445 .. 418314

Gene Structure 4870 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1006000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2442 bp

ATGCAGCAGGGTGATCAACCGGTGTTGAACTTGAGACCTGGTGGCGGTCGCGGTAGACTCCTTGGTCCTCGCTTCGACTCCTCATCCGCCGCTTCCGGCTCGTCGTCTTCATCGTCGTCTTCTTTGGCTTTCGGTTCCTTTTCTTCTGATCTCCCTCTCCTCCGTCCCCATGGCGGCGCTCCCCCTAATTTCTCTATCAAGGCTGGAGACGCACGGTTTGAGGGTCGTGAGCGAGTGAAGTACACCAGAGACCAGATCTTACAGCTTAGAGAGGCTATTGAGGTCCCTGATGAAATTCTAAAAGTGAAACAAGAAGTTGATGCTGAATTTTTCAGCGGTGAAGATCAGAGCTGGGGTCATGCAGAAACCAGTTTGCCAGCTCAATCTCCAAGTCTAGCTCCAGCTCAAAATCGTTATTCTGAGCCAGACAGCCGGGATTGGCGTGGCCGTTCTGCACCAGTTCCTACCGCTGGAGAGGAACGGTCCTGGGATACTCTTCGGGAGAATAAAGAGTCTGGTAATCGGTTTGATTACAGACAGCAAGAAGCAAATCAGTCTCAATTTTCAAGGACACAGAACCAAGGGGGAGGGCCTGCTCCCACTTTAATCAAAGCTGAGGTGCCATGGTCAGCTAGAAGGGGTACTCTATCTGATAAGGATCGCGTGTTGAAGACTGTGAAGGGGATTCTAAACAAGCTGACTCCGGAGAAGTTTGATCTCCTCAAAGGACAACTTATAGACTCTGGCATTACGTCGGCTGATATCTTAAAGGGTGTTATTTCACTGATATTTGATAAGGCCGTGCTAGAACCTACATTTTGCCCCATGTATGCTCGCCTCTGTTATGATCTTAGTGAAAAGCTTCCCCGGTTCCCCTCAGATGAACCAGATGGTAAAGAAATCACTTTCAAGCGGGTCCTCTTGAATAACTGCCAGGAGGCTTTTGATGGTGCTGACAAATTGAGGGAAGAAGTGAGACAGTTGACTGCCCCTGAACAAGAGATGGAGCGTAGGGATAAAGAAAGATTGGCAAAGATTCGTACACTGGGAAACATTCGTTTAATTGGAGAGCTTCTGAAGCAGAAGATGGTACCTGAAAAGATTGTACATCACATTGTTAAGGAGCTCTTAGGATCTGATAACAAAGTTTGTCCTGCTGAGGAAAACGTGGAAGCAATATGTCATTTTTTTAGCACTATTGGAAAGCAGCTTGATGAGAAGCCAAAATCACGCAGTATAAACGACATGTACTTCAGCCGAATGAAGGAACTGACAACAAACCCTCAATTGGCTCCAAGGTTGAGGTTCATGGTTCGAGATGTAATTGATTTGCGTTCCAACAACTGGGTTCCCAGGCGTGAAGAGATAAAAGCCAAAACCATCACTGAAATACATACGGAGGCAGAAAAAAATCTTGGATTGCGTCCGGGTGCTACTGCAAGCATCCGAAGTGGTCGTAACAATGTTTCTGGTGCTCAAGGGCAAGGGAGTACCAGCCCTGGAGGATATCCCAACAACCGACCTGGTTCAGGGGGTATGATGCCTGGAATGCCAGGAACAAGGAAGATGCCTGGGATGCCTGGAATGGATAATGACAACTGGGAGGTCCAGAGGACACGGACAATGCCAAGGGGTGATGGGTTTGGTGTGCAGCCGGGTGGTGGGCGTGGCCAGGTGCCGTTGGTTGGAAAATCAACATCAGTGAACACAAGGCTGTTACCTCAGGGTAGTGGTGTCCTTGGCAGTGGCAGGCCTAGTGCCTTGTTGCAGGGTAGTGGTGCCCCTCCTGCTCGTCCGTCCAACTATGCTTACGGTGCAGAGCCTGTAGCTCAAGTGCCAGTTCCTGCTAGACCTGTTGCAACTCCTTACGTGGCACCAGTTGTTGGAAAGCCACAAGCTCCAGCTCCAGCTCCAACCGCGGCTCCAAAGTTGAAACTAGATGAACTTCGGAGAAAAACAATCTCCCTTTTTGAGGAATATTTCAATATCGGACTCTTAGATGAAGCATTACTGTGCGTGGAGGAGCTACAATCTCCAGCTTACCACCCCGAGGTTGTCAAGGAAGGTATCGCCCTTGCCCTAGAGAAGAGTCAACCATGTGTTGAACCGCTTGGGAAGCTTCTTGAGTATTTGTTTTCTAAGAAGGTGCTTACGACCAGTGACGTAAGGAGTGGATGTTTACTCTATGCATCTATTCTGAACGATGTTGGCATTGACCTGCCAAAAGCACCAAGTAACTTTGGTCAAATTATAGGGAAATTAGTCTTGGTTGGGGGTTTGGATTTCAAGGTGGTGAAGGAGGTCCTGAAGGCGGTGGACGACGACTACTTCCAGAAAGCTATATTGGATGCTGCACTGAAGGTCGTTAGCTCTAGTCCGTCAGGGCAAGGCCTGTTGGATTCACAAGCATCTGATATTGACGCTTGCCGGAGCCTATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03013 ko05416 map03013 map05416

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

814

Amino Acids

88.71

Weight (kDa)

8.69

Isoelectric Point (pI)

56.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	223 - 448	1.3e-55	MIF4G domain
MA3	PF02847	649 - 761	3.3e-25	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003545)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57870 AT5G57870
fragaria_vesca	FvH4_1g00720 FvH4_1g00720 FvH4_2g41200
malus_domestica	MD02G1005900.v1.1 MD02G1006000.v1.1 MD08G1055400.v1.1 MD15G1150600.v1.1
prunus_persica	Prupe.1G395100_v2.0.a1 Prupe.7G265100_v2.0.a1
pyrus_communis	pycom02g00490 pycom08g04550 pycom14g07580 pycom15g13480
rosa_chinensis	RchiOBHm_Chr2g0085441 RchiOBHm_Chr6g0299311
rosa_laevigata	RLG00000011443 RLG00000015688
rosa_multiflora	Rmu_sc0007908.1_g000012 Rmu_ssc0000374.1_g000005
rosa_roxburghii	Rroxscaffold_2G00155380 Rroxscaffold_7G00168790
rosa_rugosa	Rorug01G0460400 Rorug06G0287100
rosa_samantha	Rh2AG008600 Rh2BG009900 Rh2CG010400 Rh2DG010800 Rh6AG398600 Rh6BG407000 Rh6CG413000 Rh6DG399800
rosa_wichuraiana	Rw2G000830 Rw6G034890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	63
Acc36I	ACCTGC	1 cut(s)	2226
Acc65I	GGTACC	1 cut(s)	1090
AccB1I	GGYRCC	5 cut(s)	619, 1090, 1675, 1778, 1873
AccB7I	CCANNNNNTGG	2 cut(s)	1344, 1883
AccI	GTMKAC	1 cut(s)	55
AccII	CGCG	3 cut(s)	51, 663, 1919
AccIII	TCCGGA	1 cut(s)	703
AclWI	GGATC	2 cut(s)	666, 1141
AcoI	YGGCCR	2 cut(s)	445, 1669
AcsI	RAATTY	2 cut(s)	294, 326
AcuI	CTGAAG	4 cut(s)	1097, 1237, 2327, 2378
AfaI	GTAC	7 cut(s)	242, 643, 1042, 1092, 1107, 1250, 1492
AflIII	ACRYGT	1 cut(s)	1245
AgeI	ACCGGT	1 cut(s)	19
AgsI	TTSAA	9 cut(s)	28, 567, 670, 910, 925, 1933, 1984, 2105, 2287
AjuI	GAANNNNNNNTTGG	6 cut(s)	1398, 1430, 2249, 2281, 2327, 2359
Alw21I	GWGCWC	2 cut(s)	1128, 1477
Alw26I	GTCTC	5 cut(s)	28, 201, 243, 561, 970
AlwI	GGATC	2 cut(s)	666, 1141
AlwNI	CAGNNNCTG	2 cut(s)	1823, 1844
Ama87I	CYCGRG	1 cut(s)	2048
Aor13HI	TCCGGA	1 cut(s)	703
AoxI	GGCC	6 cut(s)	445, 590, 798, 1669, 1751, 2389
ApeKI	GCWGC	4 cut(s)	4, 1207, 1651, 2351
ApoI	RAATTY	2 cut(s)	294, 326
ArsI	GACNNNNNNTTYG	2 cut(s)	200, 232
AsiGI	ACCGGT	1 cut(s)	19
Asp700I	GAANNNNTTC	1 cut(s)	861
Asp718I	GGTACC	1 cut(s)	1090
AspLEI	GCGC	1 cut(s)	179
AspS9I	GGNCC	7 cut(s)	65, 283, 483, 590, 916, 1606, 2302
AsuC2I	CCSGG	4 cut(s)	434, 868, 1428, 1656
AsuHPI	GGTGA	4 cut(s)	23, 350, 1646, 2305
AvaI	CYCGRG	1 cut(s)	2048
AvaII	GGWCC	6 cut(s)	65, 283, 483, 916, 1606, 2302
AxyI	CCTNAGG	1 cut(s)	1722
BaeGI	GKGCMC	1 cut(s)	1783
BalI	TGGCCA	1 cut(s)	1671
BanI	GGYRCC	5 cut(s)	619, 1090, 1675, 1778, 1873
BanII	GRGCYC	1 cut(s)	1128
BbsI	GAAGAC	3 cut(s)	99, 111, 677
Bbv12I	GWGCWC	2 cut(s)	1128, 1477
BbvCI	CCTCAGC	2 cut(s)	615, 1158
BbvI	GCAGC	4 cut(s)	16, 1219, 1663, 2338
BccI	CCATC	6 cut(s)	884, 941, 997, 1081, 1388, 1631
BceAI	ACGGC	3 cut(s)	432, 785, 1663
BciVI	GTATCC	1 cut(s)	484
BclI	TGATCA	1 cut(s)	13
BcnI	CCSGG	4 cut(s)	434, 868, 1428, 1656
BcoDI	GTCTC	5 cut(s)	28, 201, 243, 561, 970
BfaI	CTAG	9 cut(s)	395, 633, 806, 1755, 1848, 1937, 2081, 2373, 2440
BfmI	CTRYAG	1 cut(s)	1824
BfoI	RGCGCY	1 cut(s)	180
BfuAI	ACCTGC	1 cut(s)	2226
BfuI	GTATCC	1 cut(s)	484
BglII	AGATCT	1 cut(s)	255
BisI	GCNGC	7 cut(s)	5, 90, 175, 1208, 1652, 1920, 2352
BlsI	GCNGC	7 cut(s)	6, 91, 176, 1209, 1653, 1921, 2353
Bme18I	GGWCC	6 cut(s)	65, 283, 483, 916, 1606, 2302
BmeT110I	CYCGRG	1 cut(s)	2048
BmgT120I	GGNCC	7 cut(s)	65, 283, 483, 590, 916, 1606, 2302
BmrI	ACTGGG	3 cut(s)	1055, 1354, 1609
BmsI	GCATC	8 cut(s)	310, 1452, 1530, 1557, 1566, 2198, 2338, 2417
BmuI	ACTGGG	3 cut(s)	1055, 1354, 1609
BpiI	GAAGAC	3 cut(s)	99, 111, 677
BpmI	CTGGAG	7 cut(s)	225, 384, 492, 1521, 1884, 1890, 2019
Bpu10I	CCTNAGC	2 cut(s)	615, 1158
BpuEI	CTTGAG	4 cut(s)	52, 1461, 1815, 2144
BpuMI	CCSGG	4 cut(s)	434, 868, 1428, 1656
BsaAI	YACGTR	1 cut(s)	1870
BsaBI	GATNNNNATC	1 cut(s)	657
BsaI	GGTCTC	2 cut(s)	28, 243
BsaWI	WCCGGW	2 cut(s)	19, 703
BsaXI	ACNNNNNCTCC	4 cut(s)	1906, 1936, 2161, 2191
Bse118I	RCCGGY	1 cut(s)	19
Bse1I	ACTGG	8 cut(s)	369, 458, 1050, 1349, 1604, 1838, 1877, 2157
Bse21I	CCTNAGG	1 cut(s)	1722
Bse8I	GATNNNNATC	1 cut(s)	657
BseAI	TCCGGA	1 cut(s)	703
BseGI	GGATG	5 cut(s)	83, 1443, 1581, 2180, 2353
BseJI	GATNNNNATC	1 cut(s)	657
BseMII	CTCAG	5 cut(s)	411, 606, 891, 1149, 1736
BseNI	ACTGG	8 cut(s)	369, 458, 1050, 1349, 1604, 1838, 1877, 2157
BseRI	GAGGAG	4 cut(s)	70, 149, 710, 2036
BseSI	GKGCMC	1 cut(s)	1783
BseXI	GCAGC	4 cut(s)	16, 1219, 1663, 2338
BseYI	CCCAGC	1 cut(s)	351
BsgI	GTGCAG	4 cut(s)	438, 1670, 1836, 2337
Bsh1236I	CGCG	3 cut(s)	51, 663, 1919
Bsh1285I	CGRYCG	1 cut(s)	49
BshFI	GGCC	6 cut(s)	447, 592, 800, 1671, 1753, 2391
BshNI	GGYRCC	5 cut(s)	619, 1090, 1675, 1778, 1873
BshTI	ACCGGT	1 cut(s)	19
BsiEI	CGRYCG	1 cut(s)	49
BsiHKAI	GWGCWC	2 cut(s)	1128, 1477
BsiHKCI	CYCGRG	1 cut(s)	2048
BsiSI	CCGG	8 cut(s)	20, 96, 433, 704, 868, 1427, 1655, 2428
BslFI	GGGAC	2 cut(s)	150, 269
BsmAI	GTCTC	5 cut(s)	28, 201, 243, 561, 970
BsmBI	CGTCTC	1 cut(s)	201
BsmFI	GGGAC	2 cut(s)	150, 269
BsmI	GAATGC	1 cut(s)	1554
BsnI	GGCC	6 cut(s)	447, 592, 800, 1671, 1753, 2391
Bso31I	GGTCTC	2 cut(s)	28, 243
BsoBI	CYCGRG	1 cut(s)	2048
Bsp1286I	GDGCHC	3 cut(s)	1128, 1477, 1783
Bsp13I	TCCGGA	1 cut(s)	703
Bsp1407I	TGTACA	1 cut(s)	1105
Bsp143I	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
Bsp19I	CCATGG	2 cut(s)	169, 623
BspANI	GGCC	6 cut(s)	447, 592, 800, 1671, 1753, 2391
BspCNI	CTCAG	5 cut(s)	412, 607, 890, 1150, 1735
BspEI	TCCGGA	1 cut(s)	703
BspFNI	CGCG	3 cut(s)	51, 663, 1919
BspMI	ACCTGC	1 cut(s)	2226
BspPI	GGATC	2 cut(s)	666, 1141
BspT107I	GGYRCC	5 cut(s)	619, 1090, 1675, 1778, 1873
BspTNI	GGTCTC	2 cut(s)	28, 243
BsrFI	RCCGGY	1 cut(s)	19
BsrGI	TGTACA	1 cut(s)	1105
BsrI	ACTGG	8 cut(s)	369, 458, 1050, 1349, 1604, 1838, 1877, 2157
BssAI	RCCGGY	1 cut(s)	19
BssMI	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
BssT1I	CCWWGG	7 cut(s)	61, 169, 580, 623, 1295, 1628, 1738
Bst4CI	ACNGT	6 cut(s)	216, 483, 676, 981, 1814, 2013
Bst6I	CTCTTC	3 cut(s)	501, 1356, 2081
BstAUI	TGTACA	1 cut(s)	1105
BstBAI	YACGTR	1 cut(s)	1870
BstC8I	GCNNGC	6 cut(s)	378, 594, 835, 1441, 1751, 2425
BstDSI	CCRYGG	3 cut(s)	169, 623, 1917
BstENI	CCTNNNNNAGG	2 cut(s)	1527, 1970
BstF5I	GGATG	5 cut(s)	83, 1443, 1581, 2180, 2353
BstFNI	CGCG	3 cut(s)	51, 663, 1919
BstH2I	RGCGCY	1 cut(s)	180
BstHHI	GCGC	1 cut(s)	179
BstKTI	GATC	8 cut(s)	16, 151, 258, 346, 661, 718, 850, 1136
BstMAI	GTCTC	5 cut(s)	28, 201, 243, 561, 970
BstMBI	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
BstMCI	CGRYCG	1 cut(s)	49
BstMWI	GCNNNNNNNGC	8 cut(s)	209, 1078, 1549, 1576, 1787, 1814, 1916, 2116
BstNSI	RCATGY	1 cut(s)	1249
BstSFI	CTRYAG	1 cut(s)	1824
BstSLI	GKGCMC	1 cut(s)	1783
BstUI	CGCG	3 cut(s)	51, 663, 1919
BstV1I	GCAGC	4 cut(s)	16, 1219, 1663, 2338
BstV2I	GAAGAC	3 cut(s)	99, 111, 677
BstX2I	RGATCY	2 cut(s)	255, 1133
BstYI	RGATCY	2 cut(s)	255, 1133
Bsu36I	CCTNAGG	1 cut(s)	1722
BsuI	GTATCC	1 cut(s)	484
BsuRI	GGCC	6 cut(s)	447, 592, 800, 1671, 1753, 2391
BtgI	CCRYGG	3 cut(s)	169, 623, 1917
BtsCI	GGATG	5 cut(s)	83, 1443, 1581, 2180, 2353
BtsI	GCAGTG	1 cut(s)	1750
BtsIMutI	CAGTG	7 cut(s)	782, 1043, 1383, 1707, 1750, 2164, 2354
BveI	ACCTGC	1 cut(s)	2226
Cac8I	GCNNGC	6 cut(s)	378, 594, 835, 1441, 1751, 2425
CaiI	CAGNNNCTG	2 cut(s)	1823, 1844
CfoI	GCGC	1 cut(s)	179
Cfr10I	RCCGGY	1 cut(s)	19
Cfr13I	GGNCC	7 cut(s)	65, 283, 483, 590, 916, 1606, 2302
Cfr42I	CCGCGG	1 cut(s)	1920
CseI	GACGC	3 cut(s)	218, 1412, 2429
CsiI	ACCWGGT	2 cut(s)	37, 1522
Csp6I	GTAC	7 cut(s)	241, 642, 1041, 1091, 1106, 1249, 1491
CspAI	ACCGGT	1 cut(s)	19
CviAII	CATG	7 cut(s)	170, 359, 624, 826, 1246, 1309, 2097
CviQI	GTAC	7 cut(s)	241, 642, 1041, 1091, 1106, 1249, 1491
DpnI	GATC	8 cut(s)	15, 150, 257, 345, 660, 717, 849, 1135
DpnII	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
DrdI	GACNNNNNNGTC	1 cut(s)	63
DseDI	GACNNNNNNGTC	1 cut(s)	63
EaeI	YGGCCR	2 cut(s)	445, 1669
Eam1104I	CTCTTC	3 cut(s)	501, 1356, 2081
EarI	CTCTTC	3 cut(s)	501, 1356, 2081
EciI	GGCGGA	1 cut(s)	76
Ecl136II	GAGCTC	1 cut(s)	1126
Eco130I	CCWWGG	7 cut(s)	61, 169, 580, 623, 1295, 1628, 1738
Eco147I	AGGCCT	2 cut(s)	1753, 2391
Eco24I	GRGCYC	1 cut(s)	1128
Eco31I	GGTCTC	2 cut(s)	28, 243
Eco32I	GATATC	2 cut(s)	763, 1508
Eco47I	GGWCC	6 cut(s)	65, 283, 483, 916, 1606, 2302
Eco53kI	GAGCTC	1 cut(s)	1126
Eco57I	CTGAAG	4 cut(s)	1097, 1237, 2327, 2378
Eco81I	CCTNAGG	1 cut(s)	1722
Eco88I	CYCGRG	1 cut(s)	2048
EcoICRI	GAGCTC	1 cut(s)	1126
EcoNI	CCTNNNNNAGG	2 cut(s)	1527, 1970
EcoO109I	RGGNCCY	4 cut(s)	283, 590, 916, 2302
EcoRV	GATATC	2 cut(s)	763, 1508
EcoT14I	CCWWGG	7 cut(s)	61, 169, 580, 623, 1295, 1628, 1738
EcoT22I	ATGCAT	1 cut(s)	2191
EcoT38I	GRGCYC	1 cut(s)	1128
ErhI	CCWWGG	7 cut(s)	61, 169, 580, 623, 1295, 1628, 1738
Esp3I	CGTCTC	1 cut(s)	201
FaeI	CATG	7 cut(s)	173, 362, 627, 829, 1249, 1312, 2100
FalI	AAGNNNNNCTT	4 cut(s)	717, 749, 1112, 1144
FaqI	GGGAC	2 cut(s)	150, 269
FatI	CATG	7 cut(s)	169, 358, 623, 825, 1245, 1308, 2096
FauI	CCCGC	1 cut(s)	906
FbaI	TGATCA	1 cut(s)	13
FblI	GTMKAC	1 cut(s)	55
Fnu4HI	GCNGC	7 cut(s)	5, 90, 175, 1208, 1652, 1920, 2352
FokI	GGATG	5 cut(s)	70, 1430, 1588, 2187, 2360
FriOI	GRGCYC	1 cut(s)	1128
Fsp4HI	GCNGC	7 cut(s)	5, 90, 175, 1208, 1652, 1920, 2352
FspBI	CTAG	9 cut(s)	395, 633, 806, 1755, 1848, 1937, 2081, 2373, 2440
GlaI	GCGC	1 cut(s)	178
GluI	GCNGC	7 cut(s)	5, 90, 175, 1208, 1652, 1920, 2352
GsaI	CCCAGC	1 cut(s)	355
GsuI	CTGGAG	7 cut(s)	225, 384, 492, 1521, 1884, 1890, 2019
HaeII	RGCGCY	1 cut(s)	180
HaeIII	GGCC	6 cut(s)	447, 592, 800, 1671, 1753, 2391
HapII	CCGG	8 cut(s)	20, 96, 433, 704, 868, 1427, 1655, 2428
HgaI	GACGC	3 cut(s)	218, 1412, 2429
HhaI	GCGC	1 cut(s)	179
Hin1II	CATG	7 cut(s)	173, 362, 627, 829, 1249, 1312, 2100
Hin6I	GCGC	1 cut(s)	177
HinP1I	GCGC	1 cut(s)	177
HincII	GTYRAC	2 cut(s)	984, 2093
HindII	GTYRAC	2 cut(s)	984, 2093
HindIII	AAGCTT	2 cut(s)	860, 2117
HpaII	CCGG	8 cut(s)	20, 96, 433, 704, 868, 1427, 1655, 2428
HphI	GGTGA	4 cut(s)	23, 350, 1646, 2305
Hpy166II	GTNNAC	8 cut(s)	56, 243, 984, 1043, 1705, 2093, 2180, 2317
Hpy188III	TCNNGA	9 cut(s)	227, 500, 704, 922, 1316, 1609, 2123, 2305, 2332
Hpy8I	GTNNAC	8 cut(s)	56, 243, 984, 1043, 1705, 2093, 2180, 2317
Hpy99I	CGWCG	4 cut(s)	106, 118, 757, 2324
HpyAV	CCTTC	8 cut(s)	630, 673, 1258, 2057, 2137, 2290, 2302, 2353
HpyCH4III	ACNGT	6 cut(s)	216, 483, 676, 981, 1814, 2013
HpyCH4IV	ACGT	4 cut(s)	752, 1167, 1869, 2163
HpyF10VI	GCNNNNNNNGC	8 cut(s)	209, 1078, 1549, 1576, 1787, 1814, 1916, 2116
HpySE526I	ACGT	4 cut(s)	752, 1167, 1869, 2163
Hsp92II	CATG	7 cut(s)	173, 362, 627, 829, 1249, 1312, 2100
HspAI	GCGC	1 cut(s)	177
Kpn2I	TCCGGA	1 cut(s)	703
KpnI	GGTACC	1 cut(s)	1094
Ksp22I	TGATCA	1 cut(s)	13
KspI	CCGCGG	1 cut(s)	1920
Kzo9I	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
Lsp1109I	GCAGC	4 cut(s)	16, 1219, 1663, 2338
LweI	GCATC	8 cut(s)	310, 1452, 1530, 1557, 1566, 2198, 2338, 2417
MabI	ACCWGGT	2 cut(s)	37, 1522
MaeI	CTAG	9 cut(s)	395, 633, 806, 1755, 1848, 1937, 2081, 2373, 2440
MaeII	ACGT	4 cut(s)	752, 1167, 1869, 2163
MaeIII	GTNAC	4 cut(s)	1457, 1716, 2159, 2234
MalI	GATC	8 cut(s)	15, 150, 257, 345, 660, 717, 849, 1135
MboI	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
MfeI	CAATTG	1 cut(s)	1286
MflI	RGATCY	2 cut(s)	255, 1133
MhlI	GDGCHC	3 cut(s)	1128, 1477, 1783
MlsI	TGGCCA	1 cut(s)	1671
MluNI	TGGCCA	1 cut(s)	1671
MlyI	GAGTC	7 cut(s)	51, 71, 521, 694, 734, 1986, 2098
MmeI	TCCRAC	6 cut(s)	1362, 1666, 1824, 1864, 1937, 2376
Mox20I	TGGCCA	1 cut(s)	1671
Mph1103I	ATGCAT	1 cut(s)	2191
MroI	TCCGGA	1 cut(s)	703
MroXI	GAANNNNTTC	1 cut(s)	861
MscI	TGGCCA	1 cut(s)	1671
MseI	TTAA	4 cut(s)	605, 767, 1061, 1119
MslI	CAYNNNNRTG	1 cut(s)	2011
Msp20I	TGGCCA	1 cut(s)	1671
MspA1I	CMGCKG	3 cut(s)	336, 470, 1919
MspI	CCGG	8 cut(s)	20, 96, 433, 704, 868, 1427, 1655, 2428
MunI	CAATTG	1 cut(s)	1286
Mva1269I	GAATGC	1 cut(s)	1554
MvnI	CGCG	3 cut(s)	51, 663, 1919
MwoI	GCNNNNNNNGC	8 cut(s)	209, 1078, 1549, 1576, 1787, 1814, 1916, 2116
NciI	CCSGG	4 cut(s)	434, 868, 1428, 1656
NcoI	CCATGG	2 cut(s)	169, 623
NdeII	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
NlaIII	CATG	7 cut(s)	173, 362, 627, 829, 1249, 1312, 2100
NmuCI	GTSAC	1 cut(s)	2159
NsiI	ATGCAT	1 cut(s)	2191
NspI	RCATGY	1 cut(s)	1249
PceI	AGGCCT	2 cut(s)	1753, 2391
PciI	ACATGT	1 cut(s)	1245
PcsI	WCGNNNNNNNCGW	1 cut(s)	110
PctI	GAATGC	1 cut(s)	1554
PdmI	GAANNNNTTC	1 cut(s)	861
PfeI	GAWTC	3 cut(s)	685, 1036, 2399
PflMI	CCANNNNNTGG	2 cut(s)	1344, 1883
PinAI	ACCGGT	1 cut(s)	19
PkrI	GCNGC	7 cut(s)	6, 91, 176, 1209, 1653, 1921, 2353
PleI	GAGTC	7 cut(s)	51, 71, 520, 694, 734, 1986, 2097
PpsI	GAGTC	7 cut(s)	51, 71, 520, 694, 734, 1986, 2097
Ppu21I	YACGTR	1 cut(s)	1870
PpuMI	RGGWCCY	3 cut(s)	283, 916, 2302
PscI	ACATGT	1 cut(s)	1245
Psp124BI	GAGCTC	1 cut(s)	1128
Psp5II	RGGWCCY	3 cut(s)	283, 916, 2302
PspFI	CCCAGC	1 cut(s)	351
PspPI	GGNCC	7 cut(s)	65, 283, 483, 590, 916, 1606, 2302
PspPPI	RGGWCCY	3 cut(s)	283, 916, 2302
PstNI	CAGNNNCTG	2 cut(s)	1823, 1844
PsuI	RGATCY	2 cut(s)	255, 1133
RsaI	GTAC	7 cut(s)	242, 643, 1042, 1092, 1107, 1250, 1492
RsaNI	GTAC	7 cut(s)	241, 642, 1041, 1091, 1106, 1249, 1491
RseI	CAYNNNNRTG	1 cut(s)	2011
SacI	GAGCTC	1 cut(s)	1128
SacII	CCGCGG	1 cut(s)	1920
SaqAI	TTAA	4 cut(s)	605, 767, 1061, 1119
SatI	GCNGC	7 cut(s)	5, 90, 175, 1208, 1652, 1920, 2352
Sau3AI	GATC	8 cut(s)	13, 148, 255, 343, 658, 715, 847, 1133
Sau96I	GGNCC	7 cut(s)	65, 283, 483, 590, 916, 1606, 2302
SchI	GAGTC	7 cut(s)	51, 71, 521, 694, 734, 1986, 2098
SduI	GDGCHC	3 cut(s)	1128, 1477, 1783
SexAI	ACCWGGT	2 cut(s)	37, 1522
SfaNI	GCATC	8 cut(s)	310, 1452, 1530, 1557, 1566, 2198, 2338, 2417
SfcI	CTRYAG	1 cut(s)	1824
Sfr303I	CCGCGG	1 cut(s)	1920
SgrBI	CCGCGG	1 cut(s)	1920
SinI	GGWCC	6 cut(s)	65, 283, 483, 916, 1606, 2302
SmiMI	CAYNNNNRTG	1 cut(s)	2011
SmlI	CTYRAG	4 cut(s)	31, 1476, 1830, 2123
SmoI	CTYRAG	4 cut(s)	31, 1476, 1830, 2123
SseBI	AGGCCT	2 cut(s)	1753, 2391
SspI	AATATT	1 cut(s)	1979
SspMI	CTAG	9 cut(s)	395, 633, 806, 1755, 1848, 1937, 2081, 2373, 2440
SstI	GAGCTC	1 cut(s)	1128
StuI	AGGCCT	2 cut(s)	1753, 2391
StyI	CCWWGG	7 cut(s)	61, 169, 580, 623, 1295, 1628, 1738
TaaI	ACNGT	6 cut(s)	216, 483, 676, 981, 1814, 2013
TaiI	ACGT	4 cut(s)	755, 1170, 1872, 2166
TaqI	TCGA	2 cut(s)	75, 1315
TatI	WGTACW	3 cut(s)	240, 1105, 1248
TauI	GCSGC	3 cut(s)	92, 177, 1922
TfiI	GAWTC	3 cut(s)	685, 1036, 2399
Tru1I	TTAA	4 cut(s)	605, 767, 1061, 1119
Tru9I	TTAA	4 cut(s)	605, 767, 1061, 1119
TscAI	CASTG	7 cut(s)	789, 1050, 1390, 1707, 1750, 2164, 2361
TseFI	GTSAC	1 cut(s)	2159
TseI	GCWGC	4 cut(s)	4, 1207, 1651, 2351
Tsp45I	GTSAC	1 cut(s)	2159
TspDTI	ATGAA	7 cut(s)	99, 306, 897, 1277, 1297, 1956, 2016
TspGWI	ACGGA	5 cut(s)	152, 1412, 1633, 1785, 2367
TspRI	CASTG	7 cut(s)	789, 1050, 1390, 1707, 1750, 2164, 2361
Van91I	CCANNNNNTGG	2 cut(s)	1344, 1883
VpaK11BI	GGWCC	6 cut(s)	65, 283, 483, 916, 1606, 2302
XagI	CCTNNNNNAGG	2 cut(s)	1527, 1970
XapI	RAATTY	2 cut(s)	294, 326
XceI	RCATGY	1 cut(s)	1249
XmiI	GTMKAC	1 cut(s)	55
XmnI	GAANNNNTTC	1 cut(s)	861
XspI	CTAG	9 cut(s)	395, 633, 806, 1755, 1848, 1937, 2081, 2373, 2440
Zsp2I	ATGCAT	1 cut(s)	2191

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.