Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0003545

Physical Location & Seq

Genomic Coordinates Forward (+)

709660 .. 714968

Gene Structure 5309 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G000830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGCAGCAGGCTGATCAATCGGTGTTGAGCTTGAGACCTGGTGGCGGTCGTGGTAGACTCCTTGGTCCTCGCTTCGACTCTTCCTCCGCCCAGTCTTCTTCTCTAGCTTTCGGTTCCTTTTCTTCTGATCTCCCCACCTTGCGTCCCCATGGCGGGGCCGGCGCTGGGTTCTCTATTAAGGCCGGAGATTCGCGATTTGAGGGTCGTGACCGCGTGAAGTACACTAGAGAGCAGATCTTGCAGCTTAGAGAGGCTGTTGAGGTCCCTGATGAAATTATAAAAGTCAGACAAGAAGTTGAAGCTGAGTTTCTTCTTGCTGAAGATCAACCTTGGGTGCGTGTAGAACCCAATGTGAACAATTTGCCAAATCAACCGGCTCATTATACAGAGCCAGACAACCGGGATTGGCGCAACCGTCGTGCTCCAGTTCCTGAGACACTTAGGGAGAATAGAGAGCCTGGTAATTACAGACAGCAGGAAGCAAATCAGCAACAATATCCAAGGGCACAAAACTCTTCCAACCAAGGGGGAGGGCCTGTTCCTGCTCTAGTAAAAGCTGAGGTCCCATGGTCAGCTAGAAGGGGTACCCTATCTGACAAGGACCGTGTGGTGAAGACTGTTAAGGGGATACTGAACAAGCTGACGCCAGAGAAGTTTGATCTCCTTAAAGGACAGCTAGTAGACTCTGGAATTACCTCGGCTGATATTCTAAAGGAAGTCATTGCATTGATCTTTGATAAGGCTGTGCTGGAACCTACATTTTGTCCAATGTATGCTCGTCTATGCTCTGATCTTAATGAAAGGCTTCCCCGGTTCCCGTCAGAGGAACCAGATGGAAAAGAAATCACTTTCAAGCGTGTTCTCCTGAACAATTGCCAAGAGGCTTTTGATGGTGCTGACAAGTTGAGGGAAGAAGTGCAGCAGATGACTGCCCCTGAACAAGAGATCGAACGTAGGGATAAAGAAAGATTGGCTAAGATACGTACTCTGGGAAACATTCGTTTAATTGGAGAGCTCCTGAAGCAGAAGATGGTACCTGAAAAGATTGTCCATCACATTGTTGCGGAGCTCTTAGGAGCTGACAACAACATTTGTCCTGCTGAGGAAAATGTGGAGGCCATATGCCATTTTTTTACCACTATTGGTAAACAGCTTGATGAGAATCAAAAATCACGTCGTATAAATGATTTGTACTTCGGCCGATTGAAGGAACTGACAACAAACCCTCAGTTGGCTCCAAGGCTGAGGTTCATGGTTCGGGATGTAATTGATTTGCGCTCCAACAACTGGGTTCCCAGGCGTGAGGAGGTGAAAGCCAAAACCATCAGTGAAATACATACAGAGGCAGAAAAGAATCTTGGATTACGTCCTGGTAGCACTAGAACTGGTCGTGGCAACTTTTCTGGTACTCAAGGGAGTACCAGCCCTGGGCCCTTCTCCAACAGCCGACCTGGTTCAGGGGGCATGATGCCTGGTATGCCTGGGTTTCCAGGAACTAGGAAGATGCCTGGTATGCCTGGAATGGACAATGACAATTGGGAGGTGTCTAAGACCCGGTCAATGCCAAGAGGTGATGGTTCTGGTATGCAGCCAGCTGGGCGTGGTGGCCAGATGCCATTTATTGGCAAATCAACATCGGTGAACACAAGGCTGTTACCTCAGGGTAGTAGTGGCCTTACAAGTGGACGCCCAAGTGCCTTGTTGCAGGGTAGTGGTGCTCCTCCTGCTCGCCCAAGCAACTACTCTTTCGACACTGTGCCTGTAGCCCAGGTTCCTGTGCCTGCTAGACCTGTTCCTACTGCACCTGTATCACGACCTGTTGAAAAGCCACAAGCTCCAGCTTCAAAGTTGAAAGTAGATGAACTTAGGAAAAAAACAGTTTCTCTTTTGGAGGAATATTTCAGTGTGCGAATTTTAGATGAAGCATTATTGTGTGTGGAGGAGCTAAGGTCTCCAGCTTACCACCCTGAGGTTGTCAAAGAAGCTATCTCCCTTGCGCTGGAGAAAAGTACATCCTATGTTGAACCACTTTTTAAGCTTCTCGAGCATTTGTTCTCTAAGAAGGTCATTACAGCAAGTGACATAAGCAGTGGATGTTTGCTCTATGCATCTTTGCTGAGTGATATCGGCATTGATTATCCTAAAGCACCGAGTTATTTTGGTCAAGTTATGGGAAAATTAATTTTAGTTGGAGCTTTGGATTTTAAGGTGGTGAAAGAGGTTTTGAAGGTGGTGGATGATGACTACTATCAGAAAGCCATATTCAATGCAGGACTGCAGGTTATTAGTTCTAGCCCTTCGGGGCAAGCTGTGCTGGATTCACAAGCATCTGATATTGAGGCCTGTCAGAGTCTATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03013 ko05416 map03013 map05416

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

786

Amino Acids

86.7

Weight (kDa)

8.54

Isoelectric Point (pI)

55.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	205 - 429	2.9e-54	MIF4G domain
MA3	PF02847	622 - 734	1.7e-27	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003545)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57870 AT5G57870
fragaria_vesca	FvH4_1g00720 FvH4_1g00720 FvH4_2g41200
malus_domestica	MD02G1005900.v1.1 MD02G1006000.v1.1 MD08G1055400.v1.1 MD15G1150600.v1.1
prunus_persica	Prupe.1G395100_v2.0.a1 Prupe.7G265100_v2.0.a1
pyrus_communis	pycom02g00490 pycom08g04550 pycom14g07580 pycom15g13480
rosa_chinensis	RchiOBHm_Chr2g0085441 RchiOBHm_Chr6g0299311
rosa_laevigata	RLG00000011443 RLG00000015688
rosa_multiflora	Rmu_sc0007908.1_g000012 Rmu_ssc0000374.1_g000005
rosa_roxburghii	Rroxscaffold_2G00155380 Rroxscaffold_7G00168790
rosa_rugosa	Rorug01G0460400 Rorug06G0287100
rosa_samantha	Rh2AG008600 Rh2BG009900 Rh2CG010400 Rh2DG010800 Rh6AG398600 Rh6BG407000 Rh6CG413000 Rh6DG399800
rosa_wichuraiana	Rw2G000830 Rw6G034890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	278
AasI	GACNNNNNNGTC	1 cut(s)	63
Acc36I	ACCTGC	1 cut(s)	2269
Acc65I	GGTACC	2 cut(s)	584, 1033
AccB1I	GGYRCC	2 cut(s)	584, 1033
AccB7I	CCANNNNNTGG	2 cut(s)	1287, 1622
AccI	GTMKAC	2 cut(s)	55, 681
AccII	CGCG	2 cut(s)	193, 213
AciI	CCGC	5 cut(s)	45, 87, 153, 211, 1064
AcoI	YGGCCR	2 cut(s)	1198, 1606
AcsI	RAATTY	1 cut(s)	1911
AcuI	CTGAAG	2 cut(s)	339, 1040
AcyI	GRCGYC	2 cut(s)	644, 1687
AfaI	GTAC	8 cut(s)	221, 586, 985, 1035, 1193, 1408, 1420, 2011
AgsI	TTSAA	9 cut(s)	299, 853, 1207, 1823, 1845, 1852, 2024, 2227, 2266
AjiI	CACGTC	1 cut(s)	1175
AjuI	GAANNNNNNNTTGG	2 cut(s)	1341, 1373
AloI	GAACNNNNNNTCC	2 cut(s)	522, 554
Alw21I	GWGCWC	4 cut(s)	424, 1017, 1071, 1720
Alw26I	GTCTC	3 cut(s)	28, 428, 1956
AlwNI	CAGNNNCTG	1 cut(s)	431
Ama87I	CYCGRG	1 cut(s)	2042
AoxI	GGCC	9 cut(s)	156, 180, 533, 1116, 1198, 1430, 1606, 1672, 2340
ApaI	GGGCCC	1 cut(s)	1434
ApeKI	GCWGC	4 cut(s)	4, 241, 919, 1588
ApoI	RAATTY	1 cut(s)	1911
ArsI	GACNNNNNNTTYG	2 cut(s)	1159, 1191
AseI	ATTAAT	1 cut(s)	2180
Asp700I	GAANNNNTTC	1 cut(s)	804
Asp718I	GGTACC	2 cut(s)	584, 1033
AspLEI	GCGC	4 cut(s)	164, 411, 1278, 1999
AspS9I	GGNCC	8 cut(s)	65, 156, 262, 533, 562, 601, 1430, 1431
AsuC2I	CCSGG	3 cut(s)	401, 811, 1555
AsuHPI	GGTGA	5 cut(s)	622, 1321, 1583, 1651, 2224
AvaI	CYCGRG	1 cut(s)	2042
AvaII	GGWCC	4 cut(s)	65, 262, 562, 601
AxyI	CCTNAGG	2 cut(s)	1659, 1968
BaeGI	GKGCMC	2 cut(s)	508, 1434
BalI	TGGCCA	1 cut(s)	1608
BanI	GGYRCC	2 cut(s)	584, 1033
BanII	GRGCYC	3 cut(s)	1017, 1071, 1434
BbsI	GAAGAC	2 cut(s)	87, 620
Bbv12I	GWGCWC	4 cut(s)	424, 1017, 1071, 1720
BbvCI	CCTCAGC	3 cut(s)	558, 1101, 1244
BbvI	GCAGC	4 cut(s)	16, 253, 931, 1600
BccI	CCATC	6 cut(s)	827, 884, 1024, 1059, 1331, 1568
BciVI	GTATCC	1 cut(s)	621
BclI	TGATCA	1 cut(s)	13
BcnI	CCSGG	3 cut(s)	401, 811, 1555
BcoDI	GTCTC	3 cut(s)	28, 428, 1956
BfmI	CTRYAG	2 cut(s)	1761, 2276
BfoI	RGCGCY	1 cut(s)	165
BfuAI	ACCTGC	1 cut(s)	2269
BfuI	GTATCC	1 cut(s)	621
BglI	GCCNNNNNGGC	1 cut(s)	1597
BglII	AGATCT	1 cut(s)	234
BisI	GCNGC	4 cut(s)	5, 242, 920, 1589
BlsI	GCNGC	4 cut(s)	6, 243, 921, 1590
Bme18I	GGWCC	4 cut(s)	65, 262, 562, 601
BmeT110I	CYCGRG	1 cut(s)	2042
BmgBI	CACGTC	1 cut(s)	1175
BmgT120I	GGNCC	8 cut(s)	65, 156, 262, 533, 562, 601, 1430, 1431
BmrI	ACTGGG	2 cut(s)	85, 1297
BmsI	GCATC	5 cut(s)	1458, 1494, 1602, 2117, 2336
BmuI	ACTGGG	2 cut(s)	85, 1297
BpiI	GAAGAC	2 cut(s)	87, 620
BpmI	CTGGAG	4 cut(s)	408, 1821, 1938, 2021
Bpu10I	CCTNAGC	4 cut(s)	558, 1101, 1244, 1946
BpuEI	CTTGAG	2 cut(s)	52, 1395
BpuMI	CCSGG	3 cut(s)	401, 811, 1555
BsaAI	YACGTR	1 cut(s)	983
BsaBI	GATNNNNATC	1 cut(s)	1161
BsaHI	GRCGYC	2 cut(s)	644, 1687
BsaI	GGTCTC	2 cut(s)	28, 1956
BsaXI	ACNNNNNCTCC	6 cut(s)	68, 98, 522, 552, 1934, 1964
Bse118I	RCCGGY	2 cut(s)	158, 373
Bse1I	ACTGG	4 cut(s)	91, 425, 1292, 1390
Bse21I	CCTNAGG	2 cut(s)	1659, 1968
Bse3DI	GCAATG	1 cut(s)	720
Bse8I	GATNNNNATC	1 cut(s)	1161
BseGI	GGATG	4 cut(s)	1267, 2012, 2099, 2242
BseJI	GATNNNNATC	1 cut(s)	1161
BseMI	GCAATG	1 cut(s)	720
BseMII	CTCAG	9 cut(s)	294, 423, 549, 1092, 1235, 1241, 1673, 1959, 2108
BseNI	ACTGG	4 cut(s)	91, 425, 1292, 1390
BseRI	GAGGAG	3 cut(s)	1319, 1710, 1955
BseSI	GKGCMC	2 cut(s)	508, 1434
BseX3I	CGGCCG	1 cut(s)	1198
BseXI	GCAGC	4 cut(s)	16, 253, 931, 1600
BseYI	CCCAGC	2 cut(s)	164, 1595
BsgI	GTGCAG	2 cut(s)	938, 1785
Bsh1236I	CGCG	2 cut(s)	193, 213
Bsh1285I	CGRYCG	2 cut(s)	49, 1201
BshFI	GGCC	9 cut(s)	158, 182, 535, 1118, 1200, 1432, 1608, 1674, 2342
BshNI	GGYRCC	2 cut(s)	584, 1033
BsiEI	CGRYCG	2 cut(s)	49, 1201
BsiHKAI	GWGCWC	4 cut(s)	424, 1017, 1071, 1720
BsiHKCI	CYCGRG	1 cut(s)	2042
BsiSI	CCGG	6 cut(s)	159, 183, 374, 400, 811, 1555
BslFI	GGGAC	3 cut(s)	129, 248, 548
BsmAI	GTCTC	3 cut(s)	28, 428, 1956
BsmFI	GGGAC	3 cut(s)	129, 248, 548
BsnI	GGCC	9 cut(s)	158, 182, 535, 1118, 1200, 1432, 1608, 1674, 2342
Bso31I	GGTCTC	2 cut(s)	28, 1956
BsoBI	CYCGRG	1 cut(s)	2042
Bsp120I	GGGCCC	1 cut(s)	1430
Bsp1286I	GDGCHC	6 cut(s)	424, 508, 1017, 1071, 1434, 1720
Bsp143I	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
Bsp19I	CCATGG	2 cut(s)	148, 566
Bsp68I	TCGCGA	1 cut(s)	193
BspACI	CCGC	5 cut(s)	45, 87, 153, 211, 1064
BspANI	GGCC	9 cut(s)	158, 182, 535, 1118, 1200, 1432, 1608, 1674, 2342
BspCNI	CTCAG	9 cut(s)	295, 424, 550, 1093, 1236, 1240, 1672, 1960, 2109
BspFNI	CGCG	2 cut(s)	193, 213
BspMAI	CTGCAG	1 cut(s)	2280
BspMI	ACCTGC	1 cut(s)	2269
BspT107I	GGYRCC	2 cut(s)	584, 1033
BspTNI	GGTCTC	2 cut(s)	28, 1956
BsrDI	GCAATG	1 cut(s)	720
BsrFI	RCCGGY	2 cut(s)	158, 373
BsrI	ACTGG	4 cut(s)	91, 425, 1292, 1390
BssAI	RCCGGY	2 cut(s)	158, 373
BssMI	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
BssNI	GRCGYC	2 cut(s)	644, 1687
BssT1I	CCWWGG	7 cut(s)	61, 148, 329, 500, 523, 566, 1238
Bst4CI	ACNGT	5 cut(s)	416, 605, 619, 1756, 1879
Bst6I	CTCTTC	2 cut(s)	85, 520
BstACI	GRCGYC	2 cut(s)	644, 1687
BstAPI	GCANNNNNTGC	2 cut(s)	238, 2311
BstBAI	YACGTR	1 cut(s)	983
BstC8I	GCNNGC	6 cut(s)	9, 160, 1593, 1729, 1782, 2307
BstDSI	CCRYGG	2 cut(s)	148, 566
BstENI	CCTNNNNNAGG	1 cut(s)	1455
BstF5I	GGATG	4 cut(s)	1267, 2012, 2099, 2242
BstFNI	CGCG	2 cut(s)	193, 213
BstH2I	RGCGCY	1 cut(s)	165
BstHHI	GCGC	4 cut(s)	164, 411, 1278, 1999
BstKTI	GATC	8 cut(s)	16, 130, 237, 325, 661, 732, 793, 948
BstMAI	GTCTC	3 cut(s)	28, 428, 1956
BstMBI	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
BstMCI	CGRYCG	2 cut(s)	49, 1201
BstMWI	GCNNNNNNNGC	9 cut(s)	159, 238, 1021, 1477, 1513, 1597, 1724, 2044, 2311
BstSFI	CTRYAG	2 cut(s)	1761, 2276
BstSLI	GKGCMC	2 cut(s)	508, 1434
BstSNI	TACGTA	1 cut(s)	983
BstUI	CGCG	2 cut(s)	193, 213
BstV1I	GCAGC	4 cut(s)	16, 253, 931, 1600
BstV2I	GAAGAC	2 cut(s)	87, 620
BstX2I	RGATCY	1 cut(s)	234
BstYI	RGATCY	1 cut(s)	234
BstZI	CGGCCG	1 cut(s)	1198
Bsu36I	CCTNAGG	2 cut(s)	1659, 1968
BsuI	GTATCC	1 cut(s)	621
BsuRI	GGCC	9 cut(s)	158, 182, 535, 1118, 1200, 1432, 1608, 1674, 2342
BtgI	CCRYGG	2 cut(s)	148, 566
BtrI	CACGTC	1 cut(s)	1175
BtsCI	GGATG	4 cut(s)	1267, 2012, 2099, 2242
BtsI	GCAGTG	1 cut(s)	2095
BtsIMutI	CAGTG	4 cut(s)	1333, 1752, 1909, 2095
BtuMI	TCGCGA	1 cut(s)	193
BveI	ACCTGC	1 cut(s)	2269
Cac8I	GCNNGC	6 cut(s)	9, 160, 1593, 1729, 1782, 2307
CaiI	CAGNNNCTG	1 cut(s)	431
CfoI	GCGC	4 cut(s)	164, 411, 1278, 1999
Cfr10I	RCCGGY	2 cut(s)	158, 373
Cfr13I	GGNCC	8 cut(s)	65, 156, 262, 533, 562, 601, 1430, 1431
CseI	GACGC	3 cut(s)	131, 652, 1695
CsiI	ACCWGGT	2 cut(s)	37, 1450
Csp6I	GTAC	8 cut(s)	220, 585, 984, 1034, 1192, 1407, 1419, 2010
CviAII	CATG	4 cut(s)	149, 567, 1252, 1465
CviQI	GTAC	8 cut(s)	220, 585, 984, 1034, 1192, 1407, 1419, 2010
DpnI	GATC	8 cut(s)	15, 129, 236, 324, 660, 731, 792, 947
DpnII	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
DrdI	GACNNNNNNGTC	1 cut(s)	63
DseDI	GACNNNNNNGTC	1 cut(s)	63
EaeI	YGGCCR	2 cut(s)	1198, 1606
EagI	CGGCCG	1 cut(s)	1198
Eam1104I	CTCTTC	2 cut(s)	85, 520
EarI	CTCTTC	2 cut(s)	85, 520
EciI	GGCGGA	1 cut(s)	76
Ecl136II	GAGCTC	2 cut(s)	1015, 1069
EclXI	CGGCCG	1 cut(s)	1198
Eco105I	TACGTA	1 cut(s)	983
Eco130I	CCWWGG	7 cut(s)	61, 148, 329, 500, 523, 566, 1238
Eco147I	AGGCCT	1 cut(s)	2342
Eco24I	GRGCYC	3 cut(s)	1017, 1071, 1434
Eco31I	GGTCTC	2 cut(s)	28, 1956
Eco32I	GATATC	1 cut(s)	2125
Eco47I	GGWCC	4 cut(s)	65, 262, 562, 601
Eco52I	CGGCCG	1 cut(s)	1198
Eco53kI	GAGCTC	2 cut(s)	1015, 1069
Eco57I	CTGAAG	2 cut(s)	339, 1040
Eco81I	CCTNAGG	2 cut(s)	1659, 1968
Eco88I	CYCGRG	1 cut(s)	2042
EcoICRI	GAGCTC	2 cut(s)	1015, 1069
EcoNI	CCTNNNNNAGG	1 cut(s)	1455
EcoO109I	RGGNCCY	4 cut(s)	262, 533, 562, 1431
EcoRV	GATATC	1 cut(s)	2125
EcoT14I	CCWWGG	7 cut(s)	61, 148, 329, 500, 523, 566, 1238
EcoT22I	ATGCAT	1 cut(s)	2110
EcoT38I	GRGCYC	3 cut(s)	1017, 1071, 1434
ErhI	CCWWGG	7 cut(s)	61, 148, 329, 500, 523, 566, 1238
FaeI	CATG	4 cut(s)	152, 570, 1255, 1468
FaqI	GGGAC	3 cut(s)	129, 248, 548
FatI	CATG	4 cut(s)	148, 566, 1251, 1464
FauI	CCCGC	1 cut(s)	146
FauNDI	CATATG	1 cut(s)	1121
FbaI	TGATCA	1 cut(s)	13
FblI	GTMKAC	2 cut(s)	55, 681
Fnu4HI	GCNGC	4 cut(s)	5, 242, 920, 1589
FokI	GGATG	4 cut(s)	1274, 1999, 2106, 2249
FriOI	GRGCYC	3 cut(s)	1017, 1071, 1434
Fsp4HI	GCNGC	4 cut(s)	5, 242, 920, 1589
GlaI	GCGC	4 cut(s)	163, 410, 1277, 1998
GluI	GCNGC	4 cut(s)	5, 242, 920, 1589
GsaI	CCCAGC	2 cut(s)	168, 1599
GsuI	CTGGAG	4 cut(s)	408, 1821, 1938, 2021
HaeII	RGCGCY	1 cut(s)	165
HaeIII	GGCC	9 cut(s)	158, 182, 535, 1118, 1200, 1432, 1608, 1674, 2342
HapII	CCGG	6 cut(s)	159, 183, 374, 400, 811, 1555
HgaI	GACGC	3 cut(s)	131, 652, 1695
HhaI	GCGC	4 cut(s)	164, 411, 1278, 1999
Hin1I	GRCGYC	2 cut(s)	644, 1687
Hin1II	CATG	4 cut(s)	152, 570, 1255, 1468
Hin6I	GCGC	4 cut(s)	162, 409, 1276, 1997
HinP1I	GCGC	4 cut(s)	162, 409, 1276, 1997
HindIII	AAGCTT	1 cut(s)	2036
HinfI	GANTC	8 cut(s)	57, 77, 188, 683, 1162, 1354, 2318, 2350
HpaII	CCGG	6 cut(s)	159, 183, 374, 400, 811, 1555
HphI	GGTGA	5 cut(s)	622, 1321, 1583, 1651, 2224
Hpy166II	GTNNAC	7 cut(s)	56, 222, 355, 682, 1148, 1642, 1685
Hpy188I	TCNGA	8 cut(s)	127, 287, 595, 790, 823, 2253, 2332, 2349
Hpy188III	TCNNGA	9 cut(s)	192, 206, 431, 687, 865, 1018, 1259, 1812, 2042
Hpy8I	GTNNAC	7 cut(s)	56, 222, 355, 682, 1148, 1642, 1685
Hpy99I	CGWCG	2 cut(s)	420, 1179
HpyAV	CCTTC	6 cut(s)	573, 1201, 1444, 2056, 2221, 2307
HpyCH4III	ACNGT	5 cut(s)	416, 605, 619, 1756, 1879
HpyCH4IV	ACGT	4 cut(s)	952, 982, 1174, 1366
HpyF10VI	GCNNNNNNNGC	9 cut(s)	159, 238, 1021, 1477, 1513, 1597, 1724, 2044, 2311
HpySE526I	ACGT	4 cut(s)	952, 982, 1174, 1366
Hsp92I	GRCGYC	2 cut(s)	644, 1687
Hsp92II	CATG	4 cut(s)	152, 570, 1255, 1468
HspAI	GCGC	4 cut(s)	162, 409, 1276, 1997
KpnI	GGTACC	2 cut(s)	588, 1037
KroI	GCCGGC	1 cut(s)	158
KroNI	GCCGGC	1 cut(s)	160
Ksp22I	TGATCA	1 cut(s)	13
Kzo9I	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
Lsp1109I	GCAGC	4 cut(s)	16, 253, 931, 1600
LweI	GCATC	5 cut(s)	1458, 1494, 1602, 2117, 2336
MabI	ACCWGGT	2 cut(s)	37, 1450
MaeII	ACGT	4 cut(s)	952, 982, 1174, 1366
MaeIII	GTNAC	3 cut(s)	206, 1653, 2078
MalI	GATC	8 cut(s)	15, 129, 236, 324, 660, 731, 792, 947
MboI	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
MfeI	CAATTG	2 cut(s)	871, 1534
MflI	RGATCY	1 cut(s)	234
MhlI	GDGCHC	6 cut(s)	424, 508, 1017, 1071, 1434, 1720
MlsI	TGGCCA	1 cut(s)	1608
MluNI	TGGCCA	1 cut(s)	1608
MlyI	GAGTC	4 cut(s)	51, 71, 677, 2359
MmeI	TCCRAC	4 cut(s)	543, 1305, 1464, 2170
Mox20I	TGGCCA	1 cut(s)	1608
Mph1103I	ATGCAT	1 cut(s)	2110
MroNI	GCCGGC	1 cut(s)	158
MroXI	GAANNNNTTC	1 cut(s)	804
MscI	TGGCCA	1 cut(s)	1608
MseI	TTAA	8 cut(s)	177, 621, 666, 795, 1004, 2034, 2180, 2205
MslI	CAYNNNNRTG	1 cut(s)	1930
Msp20I	TGGCCA	1 cut(s)	1608
MspA1I	CMGCKG	1 cut(s)	1595
MspI	CCGG	6 cut(s)	159, 183, 374, 400, 811, 1555
MunI	CAATTG	2 cut(s)	871, 1534
MvnI	CGCG	2 cut(s)	193, 213
MwoI	GCNNNNNNNGC	9 cut(s)	159, 238, 1021, 1477, 1513, 1597, 1724, 2044, 2311
NaeI	GCCGGC	1 cut(s)	160
NciI	CCSGG	3 cut(s)	401, 811, 1555
NcoI	CCATGG	2 cut(s)	148, 566
NdeI	CATATG	1 cut(s)	1121
NdeII	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
NgoMIV	GCCGGC	1 cut(s)	158
NlaIII	CATG	4 cut(s)	152, 570, 1255, 1468
NmeAIII	GCCGAG	1 cut(s)	677
NmuCI	GTSAC	2 cut(s)	206, 2078
NruI	TCGCGA	1 cut(s)	193
NsiI	ATGCAT	1 cut(s)	2110
PaeR7I	CTCGAG	1 cut(s)	2042
PasI	CCCWGGG	1 cut(s)	1427
PceI	AGGCCT	1 cut(s)	2342
PdiI	GCCGGC	1 cut(s)	160
PdmI	GAANNNNTTC	1 cut(s)	804
PfeI	GAWTC	4 cut(s)	188, 1162, 1354, 2318
PflFI	GACNNNGTC	1 cut(s)	1555
PflMI	CCANNNNNTGG	2 cut(s)	1287, 1622
PfoI	TCCNGGA	1 cut(s)	1489
PkrI	GCNGC	4 cut(s)	6, 243, 921, 1590
PleI	GAGTC	4 cut(s)	51, 71, 677, 2358
PpsI	GAGTC	4 cut(s)	51, 71, 677, 2358
Ppu21I	YACGTR	1 cut(s)	983
PpuMI	RGGWCCY	2 cut(s)	262, 562
PshBI	ATTAAT	1 cut(s)	2180
PsiI	TTATAA	1 cut(s)	278
Psp124BI	GAGCTC	2 cut(s)	1017, 1071
Psp5II	RGGWCCY	2 cut(s)	262, 562
PspFI	CCCAGC	2 cut(s)	164, 1595
PspOMI	GGGCCC	1 cut(s)	1430
PspPI	GGNCC	8 cut(s)	65, 156, 262, 533, 562, 601, 1430, 1431
PspPPI	RGGWCCY	2 cut(s)	262, 562
PsrI	GAACNNNNNNTAC	2 cut(s)	1755, 1787
PstI	CTGCAG	1 cut(s)	2280
PstNI	CAGNNNCTG	1 cut(s)	431
PsuI	RGATCY	1 cut(s)	234
PsyI	GACNNNGTC	1 cut(s)	1555
PvuII	CAGCTG	1 cut(s)	1595
RruI	TCGCGA	1 cut(s)	193
RsaI	GTAC	8 cut(s)	221, 586, 985, 1035, 1193, 1408, 1420, 2011
RsaNI	GTAC	8 cut(s)	220, 585, 984, 1034, 1192, 1407, 1419, 2010
RseI	CAYNNNNRTG	1 cut(s)	1930
SacI	GAGCTC	2 cut(s)	1017, 1071
SaqAI	TTAA	8 cut(s)	177, 621, 666, 795, 1004, 2034, 2180, 2205
SatI	GCNGC	4 cut(s)	5, 242, 920, 1589
Sau3AI	GATC	8 cut(s)	13, 127, 234, 322, 658, 729, 790, 945
Sau96I	GGNCC	8 cut(s)	65, 156, 262, 533, 562, 601, 1430, 1431
SchI	GAGTC	4 cut(s)	51, 71, 677, 2359
SduI	GDGCHC	6 cut(s)	424, 508, 1017, 1071, 1434, 1720
SexAI	ACCWGGT	2 cut(s)	37, 1450
SfaNI	GCATC	5 cut(s)	1458, 1494, 1602, 2117, 2336
SfcI	CTRYAG	2 cut(s)	1761, 2276
Sfr274I	CTCGAG	1 cut(s)	2042
SinI	GGWCC	4 cut(s)	65, 262, 562, 601
SlaI	CTCGAG	1 cut(s)	2042
SmiMI	CAYNNNNRTG	1 cut(s)	1930
SmlI	CTYRAG	3 cut(s)	31, 1410, 2042
SmoI	CTYRAG	3 cut(s)	31, 1410, 2042
SnaBI	TACGTA	1 cut(s)	983
SseBI	AGGCCT	1 cut(s)	2342
SsiI	CCGC	5 cut(s)	45, 87, 153, 211, 1064
SspI	AATATT	1 cut(s)	1898
SstI	GAGCTC	2 cut(s)	1017, 1071
StuI	AGGCCT	1 cut(s)	2342
StyI	CCWWGG	7 cut(s)	61, 148, 329, 500, 523, 566, 1238
TaaI	ACNGT	5 cut(s)	416, 605, 619, 1756, 1879
TaiI	ACGT	4 cut(s)	955, 985, 1177, 1369
TaqI	TCGA	4 cut(s)	75, 948, 1749, 2043
TatI	WGTACW	3 cut(s)	219, 1191, 2009
TfiI	GAWTC	4 cut(s)	188, 1162, 1354, 2318
Tru1I	TTAA	8 cut(s)	177, 621, 666, 795, 1004, 2034, 2180, 2205
Tru9I	TTAA	8 cut(s)	177, 621, 666, 795, 1004, 2034, 2180, 2205
TscAI	CASTG	4 cut(s)	1333, 1759, 1909, 2095
TseFI	GTSAC	2 cut(s)	206, 2078
TseI	GCWGC	4 cut(s)	4, 241, 919, 1588
Tsp45I	GTSAC	2 cut(s)	206, 2078
TspDTI	ATGAA	5 cut(s)	285, 813, 1240, 1875, 1935
TspRI	CASTG	4 cut(s)	1333, 1759, 1909, 2095
Tth111I	GACNNNGTC	1 cut(s)	1555
Van91I	CCANNNNNTGG	2 cut(s)	1287, 1622
VpaK11BI	GGWCC	4 cut(s)	65, 262, 562, 601
VspI	ATTAAT	1 cut(s)	2180
XagI	CCTNNNNNAGG	1 cut(s)	1455
XapI	RAATTY	1 cut(s)	1911
XhoI	CTCGAG	1 cut(s)	2042
XmiI	GTMKAC	2 cut(s)	55, 681
XmnI	GAANNNNTTC	1 cut(s)	804
Zsp2I	ATGCAT	1 cut(s)	2110

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.