pycom14g07580

eukaryotic translation initiation factor isoform

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003545
Physical Location & Seq
Forward (+)
7744474 .. 7745691
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07580.1

Sequence Viewer

Length: 1119 bp
ATGATAGAGTGGCTAGCACCCTTTTCGCAGCCTCTTCTTATCACCCAATTTCCACTCCCACCACCACTGCCACCAGCAATCCAAACCCTTACCGCTATCCCAACGCAAGACGGTGGGGCTCATTCAGTCAAAGGCACTGTCGTCGGGATACTTTCAGTCACTTTGACTTCGATCGCAGACTCAGATCGCAGATCTGGAGCTCGTCTTCACCATCTACTGTCCCAAAGAAGGGTGGAAATCAAACCAGCGGCGATGAAAATAACAAGGCTGAGGGTTCTTGGATCGATCTTGTTCCTGCCAATTCAAGCTCAGCCTTATGCTAAGCTCGCTCGCCTCGACAAGCCGATCGGCACCTGGCTATTTGCTTGGCCTTCTTTGTGGTCAACAGCACTGGCAGCAAGTCCAGGACAGCTTCCTGATATTATGATGTTGATACTATTTGGATTTGGAGCTCTGTTTACTAGGGGCGCCGCGTGCACCATAAACGATCTCCTTGACCGGGATATTGATGCCAAGGTGGAACGTACAAAGTCGCGGCCTCTTGCAAGTGGTGTTTTGACACCTTTTCAGGGGATTTCATTTCTTGGATTTCAATTGCTCTTGGTTCTAGGAACTCTCCGTCCCCATGGCGGCGCTCCCTCCAATTTCTCTATCAAGGCTGGAGATGCACGGTTTGAGGGTCGTGAGCGAGTGAAGTACACCAGAGACCAGATCCTACAGCTTAGAGAGGTTGTTGTGCTCCCTGATGAAATTCTAAAAGTGAAACAAGAAGTTGAAGCTGAATTTTTCAGTGGTGAAGATCAGAGCCGGGGTCGTGCGGAAACAAATCTGCCAGTTCTATCTCCAACTCCATCTCCAGCTCAAAATCGTTATTCGGAGCACGACAGCCGGGATTGGTGTGGCCGTTCTACACCAATTCCTGCCTCTGGAAAGGAACGGTCTTGGGATACTCTTCGGGAGAATAAGGAGTCTGGTAATCGGTTTGATTACAGACAACAAGATGCAAATCAGGCGTCAACTGTGAGAACAACCGCGTCACGGCGTTTCGTCTTCCCGGCGTGGCTCTCTCCGGCGAAATCCCGGAGTTTGTGTTCAGTCTGCACGACTTGGTCCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 ko05416 map03013 map05416
Pfam Domains
Protein Families
MA3 MIF4G

Protein Analysis

373

Amino Acids

41.09

Weight (kDa)

9.75

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UbiA PF01040 122 - 206 6.6e-21 UbiA prenyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 350, 467
AccII CGCG 3 cut(s) 473, 535, 1034
AciI CCGC 7 cut(s) 93, 248, 471, 535, 630, 818, 1032
AclWI GGATC 2 cut(s) 289, 706
AcoI YGGCCR 1 cut(s) 901
AcsI RAATTY 2 cut(s) 750, 782
AcyI GRCGYC 2 cut(s) 468, 1013
AfaI GTAC 2 cut(s) 526, 698
AfiI CCNNNNNNNGG 7 cut(s) 228, 229, 499, 569, 629, 926, 1038
AgsI TTSAA 3 cut(s) 305, 593, 776
AjnI CCWGG 2 cut(s) 353, 403
AloI GAACNNNNNNTCC 4 cut(s) 604, 636, 1075, 1107
AluBI AGCT 8 cut(s) 200, 308, 325, 412, 452, 721, 779, 860
AluI AGCT 8 cut(s) 200, 308, 325, 412, 452, 721, 779, 860
Alw21I GWGCWC 5 cut(s) 202, 454, 479, 741, 882
Alw26I GTCTC 1 cut(s) 699
Alw44I GTGCAC 1 cut(s) 475
AlwI GGATC 2 cut(s) 289, 706
AoxI GGCC 3 cut(s) 368, 536, 901
ApaLI GTGCAC 1 cut(s) 475
ApeKI GCWGC 2 cut(s) 28, 395
ApoI RAATTY 2 cut(s) 750, 782
ArsI GACNNNNNNTTYG 4 cut(s) 123, 155, 998, 1030
AspLEI GCGC 2 cut(s) 470, 635
AspS9I GGNCC 1 cut(s) 1110
AsuC2I CCSGG 5 cut(s) 500, 809, 890, 1055, 1081
AsuHPI GGTGA 3 cut(s) 34, 200, 806
AsuNHI GCTAGC 1 cut(s) 13
AvaII GGWCC 1 cut(s) 1110
BaeGI GKGCMC 1 cut(s) 479
BanI GGYRCC 2 cut(s) 350, 467
BanII GRGCYC 3 cut(s) 121, 202, 454
BbsI GAAGAC 2 cut(s) 197, 1042
Bbv12I GWGCWC 5 cut(s) 202, 454, 479, 741, 882
BbvCI CCTCAGC 1 cut(s) 269
BbvI GCAGC 2 cut(s) 40, 407
BccI CCATC 2 cut(s) 219, 859
BceAI ACGGC 2 cut(s) 888, 1055
BcgI CGANNNNNNTGC 4 cut(s) 6, 40, 124, 158
BciT130I CCWGG 2 cut(s) 355, 405
BciVI GTATCC 2 cut(s) 141, 940
BcnI CCSGG 5 cut(s) 500, 809, 890, 1055, 1081
BcoDI GTCTC 1 cut(s) 699
BfaI CTAG 3 cut(s) 14, 462, 608
BfmI CTRYAG 1 cut(s) 716
BfoI RGCGCY 2 cut(s) 471, 636
BfuI GTATCC 2 cut(s) 141, 940
BglII AGATCT 1 cut(s) 191
BisI GCNGC 6 cut(s) 29, 249, 396, 471, 536, 631
BlpI GCTNAGC 2 cut(s) 309, 321
BlsI GCNGC 6 cut(s) 30, 250, 397, 472, 537, 632
Bme1390I CCNGG 7 cut(s) 355, 405, 500, 809, 890, 1055, 1081
Bme18I GGWCC 1 cut(s) 1110
BmgT120I GGNCC 1 cut(s) 1110
BmiI GGNNCC 2 cut(s) 352, 469
BmrFI CCNGG 7 cut(s) 355, 405, 500, 809, 890, 1055, 1081
BmsI GCATC 3 cut(s) 499, 655, 991
BmtI GCTAGC 1 cut(s) 17
BpiI GAAGAC 2 cut(s) 197, 1042
BpmI CTGGAG 3 cut(s) 216, 681, 840
Bpu10I CCTNAGC 1 cut(s) 269
Bpu1102I GCTNAGC 2 cut(s) 309, 321
BpuMI CCSGG 5 cut(s) 500, 809, 890, 1055, 1081
Bsa29I ATCGAT 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 1005
BsaHI GRCGYC 2 cut(s) 468, 1013
BsaI GGTCTC 1 cut(s) 699
BsaJI CCNNGG 3 cut(s) 513, 625, 808
BsaXI ACNNNNNCTCC 4 cut(s) 838, 868, 1075, 1105
Bsc4I CCNNNNNNNGG 7 cut(s) 228, 229, 499, 569, 629, 926, 1038
Bse1I ACTGG 2 cut(s) 396, 833
Bse8I GATNNNNATC 1 cut(s) 1005
BseBI CCWGG 2 cut(s) 355, 405
BseCI ATCGAT 1 cut(s) 284
BseDI CCNNGG 3 cut(s) 513, 625, 808
BseJI GATNNNNATC 1 cut(s) 1005
BseLI CCNNNNNNNGG 7 cut(s) 228, 229, 499, 569, 629, 926, 1038
BseMII CTCAG 3 cut(s) 195, 260, 323
BseNI ACTGG 2 cut(s) 396, 833
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 2 cut(s) 40, 407
BsgI GTGCAG 1 cut(s) 1084
Bsh1236I CGCG 3 cut(s) 473, 535, 1034
Bsh1285I CGRYCG 2 cut(s) 174, 348
BshFI GGCC 3 cut(s) 370, 538, 903
BshNI GGYRCC 2 cut(s) 350, 467
BshVI ATCGAT 1 cut(s) 284
BsiEI CGRYCG 2 cut(s) 174, 348
BsiHKAI GWGCWC 5 cut(s) 202, 454, 479, 741, 882
BsiSI CCGG 7 cut(s) 499, 808, 889, 1055, 1070, 1081, 1113
BslFI GGGAC 2 cut(s) 205, 606
BslI CCNNNNNNNGG 7 cut(s) 228, 229, 499, 569, 629, 926, 1038
BsmAI GTCTC 1 cut(s) 699
BsmFI GGGAC 2 cut(s) 205, 606
BsnI GGCC 3 cut(s) 370, 538, 903
Bso31I GGTCTC 1 cut(s) 699
Bsp1286I GDGCHC 6 cut(s) 121, 202, 454, 479, 741, 882
Bsp143I GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
Bsp1720I GCTNAGC 2 cut(s) 309, 321
Bsp19I CCATGG 1 cut(s) 625
BspACI CCGC 7 cut(s) 93, 248, 471, 535, 630, 818, 1032
BspANI GGCC 3 cut(s) 370, 538, 903
BspCNI CTCAG 3 cut(s) 194, 261, 322
BspDI ATCGAT 1 cut(s) 284
BspFNI CGCG 3 cut(s) 473, 535, 1034
BspLI GGNNCC 2 cut(s) 352, 469
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 289, 706
BspT107I GGYRCC 2 cut(s) 350, 467
BspTNI GGTCTC 1 cut(s) 699
BsrI ACTGG 2 cut(s) 396, 833
BssECI CCNNGG 3 cut(s) 513, 625, 808
BssMI GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
BssNI GRCGYC 2 cut(s) 468, 1013
BssT1I CCWWGG 2 cut(s) 513, 625
Bst2UI CCWGG 2 cut(s) 355, 405
Bst4CI ACNGT 6 cut(s) 113, 139, 219, 672, 939, 1021
Bst6I CTCTTC 2 cut(s) 39, 957
BstACI GRCGYC 2 cut(s) 468, 1013
BstC8I GCNNGC 4 cut(s) 15, 327, 331, 475
BstDEI CTNAG 5 cut(s) 181, 269, 309, 321, 722
BstDSI CCRYGG 1 cut(s) 625
BstFNI CGCG 3 cut(s) 473, 535, 1034
BstH2I RGCGCY 2 cut(s) 471, 636
BstHHI GCGC 2 cut(s) 470, 635
BstKTI GATC 9 cut(s) 174, 187, 194, 284, 288, 348, 490, 714, 802
BstMAI GTCTC 1 cut(s) 699
BstMBI GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
BstMCI CGRYCG 2 cut(s) 174, 348
BstMWI GCNNNNNNNGC 5 cut(s) 326, 395, 474, 665, 1010
BstNI CCWGG 2 cut(s) 355, 405
BstSCI CCNGG 7 cut(s) 353, 403, 498, 807, 888, 1053, 1079
BstSFI CTRYAG 1 cut(s) 716
BstSLI GKGCMC 1 cut(s) 479
BstUI CGCG 3 cut(s) 473, 535, 1034
BstV1I GCAGC 2 cut(s) 40, 407
BstV2I GAAGAC 2 cut(s) 197, 1042
BstX2I RGATCY 2 cut(s) 191, 711
BstYI RGATCY 2 cut(s) 191, 711
Bsu15I ATCGAT 1 cut(s) 284
BsuI GTATCC 2 cut(s) 141, 940
BsuRI GGCC 3 cut(s) 370, 538, 903
BsuTUI ATCGAT 1 cut(s) 284
BtgI CCRYGG 1 cut(s) 625
BtgZI GCGATG 1 cut(s) 266
BtsI GCAGTG 1 cut(s) 65
BtsIMutI CAGTG 4 cut(s) 65, 135, 389, 796
Cac8I GCNNGC 4 cut(s) 15, 327, 331, 475
CfoI GCGC 2 cut(s) 470, 635
Cfr13I GGNCC 1 cut(s) 1110
ClaI ATCGAT 1 cut(s) 284
CseI GACGC 2 cut(s) 1002, 1023
Csp6I GTAC 2 cut(s) 525, 697
CviAII CATG 1 cut(s) 626
CviQI GTAC 2 cut(s) 525, 697
DdeI CTNAG 5 cut(s) 181, 269, 309, 321, 722
DinI GGCGCC 1 cut(s) 469
DpnI GATC 9 cut(s) 173, 186, 193, 283, 287, 347, 489, 713, 801
DpnII GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
EaeI YGGCCR 1 cut(s) 901
Eam1104I CTCTTC 2 cut(s) 39, 957
EarI CTCTTC 2 cut(s) 39, 957
Ecl136II GAGCTC 2 cut(s) 200, 452
Eco130I CCWWGG 2 cut(s) 513, 625
Eco24I GRGCYC 3 cut(s) 121, 202, 454
Eco31I GGTCTC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 1110
Eco53kI GAGCTC 2 cut(s) 200, 452
EcoICRI GAGCTC 2 cut(s) 200, 452
EcoRII CCWGG 2 cut(s) 353, 403
EcoT14I CCWWGG 2 cut(s) 513, 625
EcoT38I GRGCYC 3 cut(s) 121, 202, 454
EgeI GGCGCC 1 cut(s) 469
EheI GGCGCC 1 cut(s) 469
ErhI CCWWGG 2 cut(s) 513, 625
FaeI CATG 1 cut(s) 629
FaiI YATR 4 cut(s) 318, 425, 482, 627
FaqI GGGAC 2 cut(s) 205, 606
FatI CATG 1 cut(s) 625
Fnu4HI GCNGC 6 cut(s) 29, 249, 396, 471, 536, 631
FriOI GRGCYC 3 cut(s) 121, 202, 454
Fsp4HI GCNGC 6 cut(s) 29, 249, 396, 471, 536, 631
FspBI CTAG 3 cut(s) 14, 462, 608
GlaI GCGC 2 cut(s) 469, 634
GluI GCNGC 6 cut(s) 29, 249, 396, 471, 536, 631
GsuI CTGGAG 3 cut(s) 216, 681, 840
HaeII RGCGCY 2 cut(s) 471, 636
HaeIII GGCC 3 cut(s) 370, 538, 903
HapII CCGG 7 cut(s) 499, 808, 889, 1055, 1070, 1081, 1113
HgaI GACGC 2 cut(s) 1002, 1023
HhaI GCGC 2 cut(s) 470, 635
Hin1I GRCGYC 2 cut(s) 468, 1013
Hin1II CATG 1 cut(s) 629
Hin6I GCGC 2 cut(s) 468, 633
HinP1I GCGC 2 cut(s) 468, 633
HincII GTYRAC 2 cut(s) 384, 1017
HindII GTYRAC 2 cut(s) 384, 1017
HinfI GANTC 2 cut(s) 179, 968
HpaII CCGG 7 cut(s) 499, 808, 889, 1055, 1070, 1081, 1113
HphI GGTGA 3 cut(s) 34, 200, 806
Hpy166II GTNNAC 5 cut(s) 384, 459, 477, 699, 1017
Hpy188I TCNGA 3 cut(s) 184, 804, 877
Hpy188III TCNNGA 6 cut(s) 145, 195, 416, 683, 927, 956
Hpy8I GTNNAC 5 cut(s) 384, 459, 477, 699, 1017
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 2 cut(s) 222, 381
HpyCH4III ACNGT 6 cut(s) 113, 139, 219, 672, 939, 1021
HpyCH4IV ACGT 1 cut(s) 523
HpyCH4V TGCA 5 cut(s) 477, 545, 668, 1004, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 326, 395, 474, 665, 1010
HpyF3I CTNAG 5 cut(s) 181, 269, 309, 321, 722
HpySE526I ACGT 1 cut(s) 523
Hsp92I GRCGYC 2 cut(s) 468, 1013
Hsp92II CATG 1 cut(s) 629
HspAI GCGC 2 cut(s) 468, 633
KasI GGCGCC 1 cut(s) 467
Kzo9I GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
LmnI GCTCC 5 cut(s) 197, 449, 640, 744, 877
Lsp1109I GCAGC 2 cut(s) 40, 407
LweI GCATC 3 cut(s) 499, 655, 991
MaeI CTAG 3 cut(s) 14, 462, 608
MaeII ACGT 1 cut(s) 523
MaeIII GTNAC 2 cut(s) 157, 1035
MalI GATC 9 cut(s) 173, 186, 193, 283, 287, 347, 489, 713, 801
MboI GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
MboII GAAGA 5 cut(s) 26, 197, 809, 944, 1042
MfeI CAATTG 1 cut(s) 593
MflI RGATCY 2 cut(s) 191, 711
MhlI GDGCHC 6 cut(s) 121, 202, 454, 479, 741, 882
MluCI AATT 7 cut(s) 47, 300, 593, 643, 750, 782, 915
Mly113I GGCGCC 1 cut(s) 468
MlyI GAGTC 2 cut(s) 173, 977
MmeI TCCRAC 1 cut(s) 869
MnlI CCTC 8 cut(s) 42, 264, 344, 549, 649, 670, 721, 934
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 7 cut(s) 499, 808, 889, 1055, 1070, 1081, 1113
MspR9I CCNGG 7 cut(s) 355, 405, 500, 809, 890, 1055, 1081
MunI CAATTG 1 cut(s) 593
MvaI CCWGG 2 cut(s) 355, 405
MvnI CGCG 3 cut(s) 473, 535, 1034
MwoI GCNNNNNNNGC 5 cut(s) 326, 395, 474, 665, 1010
NarI GGCGCC 1 cut(s) 468
NciI CCSGG 5 cut(s) 500, 809, 890, 1055, 1081
NcoI CCATGG 1 cut(s) 625
NdeII GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 629
NlaIV GGNNCC 2 cut(s) 352, 469
NmuCI GTSAC 2 cut(s) 157, 1035
PcsI WCGNNNNNNNCGW 1 cut(s) 333
PflFI GACNNNGTC 1 cut(s) 1108
PfoI TCCNGGA 2 cut(s) 403, 1079
PkrI GCNGC 6 cut(s) 30, 250, 397, 472, 537, 632
Ple19I CGATCG 2 cut(s) 174, 348
PleI GAGTC 2 cut(s) 173, 976
PluTI GGCGCC 1 cut(s) 471
PpsI GAGTC 2 cut(s) 173, 976
Psp124BI GAGCTC 2 cut(s) 202, 454
Psp6I CCWGG 2 cut(s) 353, 403
PspGI CCWGG 2 cut(s) 353, 403
PspN4I GGNNCC 2 cut(s) 352, 469
PspPI GGNCC 1 cut(s) 1110
PsuI RGATCY 2 cut(s) 191, 711
PsyI GACNNNGTC 1 cut(s) 1108
PvuI CGATCG 2 cut(s) 174, 348
RsaI GTAC 2 cut(s) 526, 698
RsaNI GTAC 2 cut(s) 525, 697
SacI GAGCTC 2 cut(s) 202, 454
SatI GCNGC 6 cut(s) 29, 249, 396, 471, 536, 631
Sau3AI GATC 9 cut(s) 171, 184, 191, 281, 285, 345, 487, 711, 799
Sau96I GGNCC 1 cut(s) 1110
SchI GAGTC 2 cut(s) 173, 977
ScrFI CCNGG 7 cut(s) 355, 405, 500, 809, 890, 1055, 1081
SduI GDGCHC 6 cut(s) 121, 202, 454, 479, 741, 882
SfaNI GCATC 3 cut(s) 499, 655, 991
SfcI CTRYAG 1 cut(s) 716
SfoI GGCGCC 1 cut(s) 469
SinI GGWCC 1 cut(s) 1110
Sse9I AATT 7 cut(s) 47, 300, 593, 643, 750, 782, 915
SsiI CCGC 7 cut(s) 93, 248, 471, 535, 630, 818, 1032
SspDI GGCGCC 1 cut(s) 467
SspMI CTAG 3 cut(s) 14, 462, 608
SstI GAGCTC 2 cut(s) 202, 454
StyD4I CCNGG 7 cut(s) 353, 403, 498, 807, 888, 1053, 1079
StyI CCWWGG 2 cut(s) 513, 625
TaaI ACNGT 6 cut(s) 113, 139, 219, 672, 939, 1021
TaiI ACGT 1 cut(s) 526
TaqI TCGA 3 cut(s) 170, 284, 336
TasI AATT 7 cut(s) 47, 300, 593, 643, 750, 782, 915
TatI WGTACW 1 cut(s) 696
TauI GCSGC 4 cut(s) 251, 473, 538, 633
TscAI CASTG 4 cut(s) 72, 142, 396, 796
TseFI GTSAC 2 cut(s) 157, 1035
TseI GCWGC 2 cut(s) 28, 395
Tsp45I GTSAC 2 cut(s) 157, 1035
TspDTI ATGAA 3 cut(s) 269, 567, 762
TspGWI ACGGA 1 cut(s) 608
TspRI CASTG 4 cut(s) 72, 142, 396, 796
Tth111I GACNNNGTC 1 cut(s) 1108
VneI GTGCAC 1 cut(s) 475
VpaK11BI GGWCC 1 cut(s) 1110
XapI RAATTY 2 cut(s) 750, 782
XspI CTAG 3 cut(s) 14, 462, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.