Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0003545

Physical Location & Seq

Genomic Coordinates Reverse (-)

9580759 .. 9586953

Gene Structure 6195 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00168790.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2475 bp

ATGATGGCGGATCCGACTGTGATAAGTTTGAGGCCCGGCGGCGCTATTGGCCTGCGCGCCCCACGATTCCTCACACCTCGCCTCGATTCCTCTTCAGAGCCTCAATCCTCCAATGCTGCTCTCTCTTCCGCCTTCAAGGTCCCGCCTGAACCTAACAATCTTATTGATATCTATATCAATTATACTGGTGACTTGCGGTTTGAAGGTCGTGACCGTGTTCGATATACTAGAGATCAGCTAATGCAGCTTAGAGAGGCTGCCACTACTATCCCCGAGGGTATTTTGAAGATTAAACAAGAAATAGAGGCCGAGTTTGGTGGAGATGATCCTACTTGGGGACGCACAGATGCCAACCCACAGCCTCAACCTCAGCCTCAATTGCAAGGTCGTTATTCCGAGTTAGATAACCGTGATTGGCGTGGTCGATCTGGACAGTTACCTGCTTCTGAAGAGGAAAGAAATGATTCTTCAAGGCGACAAGGATTGAATAACCAGCAGGACCAACAATTTGCAAGGCCACAAATTTCTGGTAACCAAGTGACTGTAAGAGGACCTGCTCCTGTTCTCCTTAAGGCGGAGGTTCCATGGTCAGCTCGAAGAGCAAATCTGTCTGAGAAGGATCGTGTATTGAAGACAGTGAAAGGTATACTGAACAAATTGACCCCAGAGAAGTTTGATGTTCTCAAGGGTCAACTAATTGACTCTGGAATCACAAATCCAGATATTTTGAAGGATGTTATCTTTCTGATATTTGATAAGGCTGTGCTGGAGCCAACATTTTGTCCAATGTATGCCCTTTTATGTGCGGATCTTAATGCGAAACTTCCTCCTTTTCCACCTGAGGAGCCTGGGGGGAAAGAAATCACTTTTAAACGAGTCCTGTTGAATAACTGCCAGGAGGCTTTCGAAGGTGCTGACAACCTGAGGGCAGAAATTAGGCAGATGACTGCTCCTGAGCAAGAAATGGAATGCAGAGATAAAGAACGAATGCTTAAGTTGCGAACTCTAGGAAACATACGCCTTATTGGGGAACTTTTAAAGCAGAAGATGGTGCCTGAAAAGATTGTACACCATATTGTTCAGGAGCTTCTGGGATATGATGGAAAAACATGCCCTGCTGAAGAGAATGTAGAGGCAATATGTCAGTTTTTCAACACCATTGGTAAGCAGCTTGATGAGAGTCAAAAATCTCGCCGAATCAACGATACCTATTTCAGTAGGTTGAAGGAACTGACTACAAACTCCCAACTTGCACCACGGTTGAGGTTCATGGTCCGCAATGTTCTTGATATGCGGACCAACAACTGGATTCCAAGACGGGAAGAGGTGAAAGCAAAGACTATAACTGAAATTCATTCAGAAGCAGAAAAGAACTTAGGGTTGCGTCCAGGTGCAACGGCAACGATAAGGAATGCTCGTATTGCAGCTGCCTCTGGGGATTTGAGCCCCGGAGGTCTTCCTGCTACCCGGCCTGGCTATATGCCTGGAATGCCTGGGAGCAGGAAAATGCCGGGAATGCCTGGACTTGATTCTGACAATTGGGAGGTTTATCGATCTAGATCAATGACCAAGCGCGATGGTTTGGTTAACTCTCCATCTGGAGGACGTGTCCAGCCACCGTTGATCAGCAAAGCACCGTCTCTTAATTCAAAATTTTTACCTCAAGGCAGTGGTGGAATCATAACTGGGAAAACAAGTGCCTTGTTGCAGGGCTCTGGTGGTCCTCCTCGAACTAATTTGATTACTGGAGTTGAACCTCTTACTCAAAACGCCAAGCCTACAGTTCCAGCTGCATCATCAGTTTCTTCTGAGAAGGCTCTGGCCCCTGAAATTGTACCGACTTCTGCTAAACCTCTGATTCCTGCAACAGTACCAAATTCTGCTGCTCTTCTTAAAAAAACTGTATCCCTGCTGGAAGAATATTTCAGTGTTCGGCTTCTCGACGAGGCACTTCAATGTGTGGAGGAGCTGAAAGCCCCAGCCTACCATCCGGAGTTTGTTAAAGAAGCTATTGCTCTTGCTCTGGATAAGAGCCCACCATGTGTAGAACCGGTCATAAAACTTCTTGAGTACCTGTTCAGTAAAAACGTTTTGACATCGAGAGATATAGGGACTGGTTGCCTTTCATATGGGTCAATGTTGGACGAAATCGGCATTGATTTGCCGAAAGCACCAAATAATTTTGGTGACATTATGGGGAAGTTGGCTCTCGCAGGGGCATTGGACTTCAAGGTTGTGAAGGAAATCCTTAAGAAGGTGGAAGATGAGATGTATCGGTCGGCCATTTTTGGTGCTGCTATGAAGAGCATTACATCGCAAGATTTTTGGCTAGCCAGGAAACTGAAATTCAGGCATGTGAAAGTTTGCTTTCCTGATTCAGGCCAGTTTCTATGGCCACTGGAAAACATTTTCGCCCTTGTGACATTTGGCTACAGGGGAATAGGGAGTAGATTTGCTTATTTCCCATATTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03013 ko05416 map03013 map05416

Pfam Domains

Protein Families

MA3 MIF4G

Protein Analysis

824

Amino Acids

91.3

Weight (kDa)

8.93

Isoelectric Point (pI)

50.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MIF4G	PF02854	210 - 435	1.7e-53	MIF4G domain
MA3	PF02847	632 - 742	4.2e-25	MA3 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003545)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G57870 AT5G57870
fragaria_vesca	FvH4_1g00720 FvH4_1g00720 FvH4_2g41200
malus_domestica	MD02G1005900.v1.1 MD02G1006000.v1.1 MD08G1055400.v1.1 MD15G1150600.v1.1
prunus_persica	Prupe.1G395100_v2.0.a1 Prupe.7G265100_v2.0.a1
pyrus_communis	pycom02g00490 pycom08g04550 pycom14g07580 pycom15g13480
rosa_chinensis	RchiOBHm_Chr2g0085441 RchiOBHm_Chr6g0299311
rosa_laevigata	RLG00000011443 RLG00000015688
rosa_multiflora	Rmu_sc0007908.1_g000012 Rmu_ssc0000374.1_g000005
rosa_roxburghii	Rroxscaffold_2G00155380 Rroxscaffold_7G00168790
rosa_rugosa	Rorug01G0460400 Rorug06G0287100
rosa_samantha	Rh2AG008600 Rh2BG009900 Rh2CG010400 Rh2DG010800 Rh6AG398600 Rh6BG407000 Rh6CG413000 Rh6DG399800
rosa_wichuraiana	Rw2G000830 Rw6G034890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	448, 562
AccB1I	GGYRCC	1 cut(s)	1051
AccB7I	CCANNNNNTGG	1 cut(s)	1305
AccI	GTMKAC	1 cut(s)	646
AccII	CGCG	2 cut(s)	57, 1575
AccIII	TCCGGA	1 cut(s)	1990
AciI	CCGC	9 cut(s)	8, 39, 129, 143, 196, 575, 806, 1276, 1294
AclI	AACGTT	1 cut(s)	2088
AclWI	GGATC	5 cut(s)	5, 18, 320, 627, 816
AcoI	YGGCCR	2 cut(s)	2280, 2393
AcsI	RAATTY	5 cut(s)	522, 1350, 1652, 1876, 2345
AcuI	CTGAAG	3 cut(s)	78, 468, 1140
AdeI	CACNNNGTG	1 cut(s)	2042
AfaI	GTAC	4 cut(s)	1068, 1836, 1872, 2072
AfiI	CCNNNNNNNGG	7 cut(s)	335, 574, 1027, 1305, 1601, 2254, 2378
AflII	CTTAAG	3 cut(s)	569, 992, 2249
AflIII	ACRYGT	1 cut(s)	1606
AgeI	ACCGGT	1 cut(s)	2050
AjiI	CACGTC	1 cut(s)	1607
AjnI	CCWGG	8 cut(s)	847, 894, 1387, 1471, 1483, 1492, 1519, 2333
AluBI	AGCT	9 cut(s)	238, 247, 593, 1087, 1171, 1427, 1790, 1969, 2009
AluI	AGCT	9 cut(s)	238, 247, 593, 1087, 1171, 1427, 1790, 1969, 2009
Alw26I	GTCTC	1 cut(s)	1644
AlwI	GGATC	5 cut(s)	5, 18, 320, 627, 816
Ama87I	CYCGRG	1 cut(s)	272
Aor13HI	TCCGGA	1 cut(s)	1990
AoxI	GGCC	9 cut(s)	32, 49, 306, 515, 1469, 1821, 2280, 2380, 2393
ApeKI	GCWGC	9 cut(s)	116, 244, 257, 1168, 1424, 1427, 1790, 1883, 2294
ApoI	RAATTY	5 cut(s)	522, 1350, 1652, 1876, 2345
ArsI	GACNNNNNNTTYG	4 cut(s)	182, 214, 491, 523
AsiGI	ACCGGT	1 cut(s)	2050
Asp700I	GAANNNNTTC	1 cut(s)	463
AspLEI	GCGC	4 cut(s)	44, 57, 59, 1575
AspS9I	GGNCC	8 cut(s)	33, 139, 499, 551, 1273, 1296, 1721, 1822
AsuC2I	CCSGG	4 cut(s)	36, 1449, 1468, 1512
AsuHPI	GGTGA	3 cut(s)	200, 1339, 2198
AsuII	TTCGAA	1 cut(s)	906
AsuNHI	GCTAGC	1 cut(s)	2329
AvaI	CYCGRG	1 cut(s)	272
AvaII	GGWCC	6 cut(s)	139, 499, 551, 1273, 1296, 1721
AxyI	CCTNAGG	2 cut(s)	840, 923
BalI	TGGCCA	1 cut(s)	2395
BamHI	GGATCC	1 cut(s)	10
BanI	GGYRCC	1 cut(s)	1051
BanII	GRGCYC	3 cut(s)	1448, 1715, 2036
BbsI	GAAGAC	2 cut(s)	638, 1448
BbvCI	CCTCAGC	1 cut(s)	369
BbvI	GCAGC	9 cut(s)	103, 244, 256, 1180, 1414, 1436, 1777, 1870, 2281
BccI	CCATC	5 cut(s)	1042, 1094, 1571, 1603, 1995
BceAI	ACGGC	1 cut(s)	1413
BciT130I	CCWGG	8 cut(s)	849, 896, 1389, 1473, 1485, 1494, 1521, 2335
BciVI	GTATCC	1 cut(s)	1915
BclI	TGATCA	1 cut(s)	1623
BcnI	CCSGG	4 cut(s)	36, 1449, 1468, 1512
BcoDI	GTCTC	1 cut(s)	1644
BfaI	CTAG	4 cut(s)	228, 1007, 1557, 2330
BfmI	CTRYAG	2 cut(s)	1779, 2431
BfoI	RGCGCY	1 cut(s)	45
BfrI	CTTAAG	3 cut(s)	569, 992, 2249
BfuAI	ACCTGC	2 cut(s)	448, 562
BfuI	GTATCC	1 cut(s)	1915
Bme18I	GGWCC	6 cut(s)	139, 499, 551, 1273, 1296, 1721
BmeT110I	CYCGRG	1 cut(s)	272
BmgBI	CACGTC	1 cut(s)	1607
BmgT120I	GGNCC	8 cut(s)	33, 139, 499, 551, 1273, 1296, 1721, 1822
BmiI	GGNNCC	7 cut(s)	12, 141, 582, 771, 846, 1053, 1824
BmrI	ACTGGG	1 cut(s)	1695
BmsI	GCATC	2 cut(s)	337, 1802
BmtI	GCTAGC	1 cut(s)	2333
BmuI	ACTGGG	1 cut(s)	1695
BpiI	GAAGAC	2 cut(s)	638, 1448
BpmI	CTGGAG	3 cut(s)	788, 1620, 1767
Bpu10I	CCTNAGC	2 cut(s)	369, 954
Bpu14I	TTCGAA	1 cut(s)	906
BpuEI	CTTGAG	3 cut(s)	668, 1647, 2087
BpuMI	CCSGG	4 cut(s)	36, 1449, 1468, 1512
Bsa29I	ATCGAT	1 cut(s)	1552
BsaBI	GATNNNNATC	1 cut(s)	1558
BsaJI	CCNNGG	6 cut(s)	273, 584, 848, 1256, 1447, 1493
BsaWI	WCCGGW	2 cut(s)	1990, 2050
BsaXI	ACNNNNNCTCC	2 cut(s)	1593, 1623
Bsc4I	CCNNNNNNNGG	7 cut(s)	335, 574, 1027, 1305, 1601, 2254, 2378
Bse118I	RCCGGY	1 cut(s)	2050
Bse1I	ACTGG	7 cut(s)	190, 1310, 1690, 1750, 2119, 2383, 2403
Bse21I	CCTNAGG	2 cut(s)	840, 923
Bse3DI	GCAATG	1 cut(s)	1285
Bse8I	GATNNNNATC	1 cut(s)	1558
BseAI	TCCGGA	1 cut(s)	1990
BseBI	CCWGG	8 cut(s)	849, 896, 1389, 1473, 1485, 1494, 1521, 2335
BseCI	ATCGAT	1 cut(s)	1552
BseDI	CCNNGG	6 cut(s)	273, 584, 848, 1256, 1447, 1493
BseGI	GGATG	2 cut(s)	739, 1987
BseJI	GATNNNNATC	1 cut(s)	1558
BseLI	CCNNNNNNNGG	7 cut(s)	335, 574, 1027, 1305, 1601, 2254, 2378
BseMI	GCAATG	1 cut(s)	1285
BseMII	CTCAG	6 cut(s)	383, 603, 831, 914, 945, 1800
BseNI	ACTGG	7 cut(s)	190, 1310, 1690, 1750, 2119, 2383, 2403
BsePI	GCGCGC	1 cut(s)	55
BseRI	GAGGAG	3 cut(s)	857, 1716, 1979
BseXI	GCAGC	9 cut(s)	103, 244, 256, 1180, 1414, 1436, 1777, 1870, 2281
BseYI	CCCAGC	1 cut(s)	1978
Bsh1236I	CGCG	2 cut(s)	57, 1575
Bsh1285I	CGRYCG	1 cut(s)	2279
BshFI	GGCC	9 cut(s)	34, 51, 308, 517, 1471, 1823, 2282, 2382, 2395
BshNI	GGYRCC	1 cut(s)	1051
BshTI	ACCGGT	1 cut(s)	2050
BshVI	ATCGAT	1 cut(s)	1552
BsiEI	CGRYCG	1 cut(s)	2279
BsiHKCI	CYCGRG	1 cut(s)	272
BsiSI	CCGG	6 cut(s)	36, 1449, 1468, 1511, 1991, 2051
BslFI	GGGAC	3 cut(s)	125, 351, 2125
BslI	CCNNNNNNNGG	7 cut(s)	335, 574, 1027, 1305, 1601, 2254, 2378
BsmAI	GTCTC	1 cut(s)	1644
BsmBI	CGTCTC	1 cut(s)	1644
BsmFI	GGGAC	3 cut(s)	125, 351, 2125
BsmI	GAATGC	5 cut(s)	974, 993, 1417, 1494, 1521
BsnI	GGCC	9 cut(s)	34, 51, 308, 517, 1471, 1823, 2282, 2382, 2395
BsoBI	CYCGRG	1 cut(s)	272
Bsp119I	TTCGAA	1 cut(s)	906
Bsp1286I	GDGCHC	3 cut(s)	1448, 1715, 2036
Bsp13I	TCCGGA	1 cut(s)	1990
Bsp1407I	TGTACA	1 cut(s)	1066
Bsp143I	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
Bsp19I	CCATGG	1 cut(s)	584
BspACI	CCGC	9 cut(s)	8, 39, 129, 143, 196, 575, 806, 1276, 1294
BspANI	GGCC	9 cut(s)	34, 51, 308, 517, 1471, 1823, 2282, 2382, 2395
BspCNI	CTCAG	6 cut(s)	382, 604, 832, 915, 946, 1801
BspDI	ATCGAT	1 cut(s)	1552
BspEI	TCCGGA	1 cut(s)	1990
BspFNI	CGCG	2 cut(s)	57, 1575
BspLI	GGNNCC	7 cut(s)	12, 141, 582, 771, 846, 1053, 1824
BspMI	ACCTGC	2 cut(s)	448, 562
BspOI	GCTAGC	1 cut(s)	2333
BspPI	GGATC	5 cut(s)	5, 18, 320, 627, 816
BspQI	GCTCTTC	3 cut(s)	592, 1893, 2297
BspT104I	TTCGAA	1 cut(s)	906
BspT107I	GGYRCC	1 cut(s)	1051
BspTI	CTTAAG	3 cut(s)	569, 992, 2249
BsrDI	GCAATG	1 cut(s)	1285
BsrFI	RCCGGY	1 cut(s)	2050
BsrGI	TGTACA	1 cut(s)	1066
BsrI	ACTGG	7 cut(s)	190, 1310, 1690, 1750, 2119, 2383, 2403
BssAI	RCCGGY	1 cut(s)	2050
BssECI	CCNNGG	6 cut(s)	273, 584, 848, 1256, 1447, 1493
BssHII	GCGCGC	1 cut(s)	55
BssMI	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
BssNAI	GTATAC	1 cut(s)	647
BssT1I	CCWWGG	1 cut(s)	584
Bst1107I	GTATAC	1 cut(s)	647
Bst2UI	CCWGG	8 cut(s)	849, 896, 1389, 1473, 1485, 1494, 1521, 2335
Bst6I	CTCTTC	8 cut(s)	97, 130, 444, 592, 1116, 1317, 1893, 2297
BstAFI	CTTAAG	3 cut(s)	569, 992, 2249
BstAUI	TGTACA	1 cut(s)	1066
BstBI	TTCGAA	1 cut(s)	906
BstC8I	GCNNGC	3 cut(s)	53, 57, 2331
BstDEI	CTNAG	8 cut(s)	248, 369, 612, 840, 923, 954, 1375, 1809
BstDSI	CCRYGG	2 cut(s)	584, 1256
BstEII	GGTNACC	1 cut(s)	530
BstENI	CCTNNNNNAGG	2 cut(s)	2252, 2376
BstF5I	GGATG	2 cut(s)	739, 1987
BstFNI	CGCG	2 cut(s)	57, 1575
BstH2I	RGCGCY	1 cut(s)	45
BstHHI	GCGC	4 cut(s)	44, 57, 59, 1575
BstKTI	GATC	9 cut(s)	13, 235, 328, 428, 622, 811, 1556, 1562, 1626
BstMAI	GTCTC	1 cut(s)	1644
BstMBI	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
BstMCI	CGRYCG	1 cut(s)	2279
BstMWI	GCNNNNNNNGC	8 cut(s)	48, 244, 379, 599, 997, 1421, 1489, 1516
BstNI	CCWGG	8 cut(s)	849, 896, 1389, 1473, 1485, 1494, 1521, 2335
BstNSI	RCATGY	2 cut(s)	1113, 2357
BstPI	GGTNACC	1 cut(s)	530
BstSFI	CTRYAG	2 cut(s)	1779, 2431
BstUI	CGCG	2 cut(s)	57, 1575
BstV1I	GCAGC	9 cut(s)	103, 244, 256, 1180, 1414, 1436, 1777, 1870, 2281
BstV2I	GAAGAC	2 cut(s)	638, 1448
BstX2I	RGATCY	2 cut(s)	10, 808
BstYI	RGATCY	2 cut(s)	10, 808
BstZ17I	GTATAC	1 cut(s)	647
Bsu15I	ATCGAT	1 cut(s)	1552
Bsu36I	CCTNAGG	2 cut(s)	840, 923
BsuI	GTATCC	1 cut(s)	1915
BsuRI	GGCC	9 cut(s)	34, 51, 308, 517, 1471, 1823, 2282, 2382, 2395
BsuTUI	ATCGAT	1 cut(s)	1552
BtgI	CCRYGG	2 cut(s)	584, 1256
BtgZI	GCGATG	2 cut(s)	1590, 2298
BtrI	CACGTC	1 cut(s)	1607
BtsCI	GGATG	2 cut(s)	739, 1987
BtsI	GCAGTG	1 cut(s)	1675
BtsIMutI	CAGTG	4 cut(s)	642, 1675, 1933, 2396
BveI	ACCTGC	2 cut(s)	448, 562
Cac8I	GCNNGC	3 cut(s)	53, 57, 2331
CfoI	GCGC	4 cut(s)	44, 57, 59, 1575
Cfr10I	RCCGGY	1 cut(s)	2050
Cfr13I	GGNCC	8 cut(s)	33, 139, 499, 551, 1273, 1296, 1721, 1822
ClaI	ATCGAT	1 cut(s)	1552
CseI	GACGC	2 cut(s)	348, 1373
Csp6I	GTAC	4 cut(s)	1067, 1835, 1871, 2071
CspAI	ACCGGT	1 cut(s)	2050
CviAII	CATG	5 cut(s)	585, 1110, 1270, 2040, 2354
CviQI	GTAC	4 cut(s)	1067, 1835, 1871, 2071
DdeI	CTNAG	8 cut(s)	248, 369, 612, 840, 923, 954, 1375, 1809
DpnI	GATC	9 cut(s)	12, 234, 327, 427, 621, 810, 1555, 1561, 1625
DpnII	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
DraI	TTTAAA	2 cut(s)	871, 1038
DraIII	CACNNNGTG	1 cut(s)	2042
EaeI	YGGCCR	2 cut(s)	2280, 2393
Eam1104I	CTCTTC	8 cut(s)	97, 130, 444, 592, 1116, 1317, 1893, 2297
EarI	CTCTTC	8 cut(s)	97, 130, 444, 592, 1116, 1317, 1893, 2297
EciI	GGCGGA	3 cut(s)	23, 118, 590
Eco130I	CCWWGG	1 cut(s)	584
Eco24I	GRGCYC	3 cut(s)	1448, 1715, 2036
Eco32I	GATATC	1 cut(s)	169
Eco47I	GGWCC	6 cut(s)	139, 499, 551, 1273, 1296, 1721
Eco57I	CTGAAG	3 cut(s)	78, 468, 1140
Eco81I	CCTNAGG	2 cut(s)	840, 923
Eco88I	CYCGRG	1 cut(s)	272
Eco91I	GGTNACC	1 cut(s)	530
EcoNI	CCTNNNNNAGG	2 cut(s)	2252, 2376
EcoO109I	RGGNCCY	2 cut(s)	139, 551
EcoO65I	GGTNACC	1 cut(s)	530
EcoRII	CCWGG	8 cut(s)	847, 894, 1387, 1471, 1483, 1492, 1519, 2333
EcoRV	GATATC	1 cut(s)	169
EcoT14I	CCWWGG	1 cut(s)	584
EcoT38I	GRGCYC	3 cut(s)	1448, 1715, 2036
ErhI	CCWWGG	1 cut(s)	584
Esp3I	CGTCTC	1 cut(s)	1644
FaeI	CATG	5 cut(s)	588, 1113, 1273, 2043, 2357
FaqI	GGGAC	3 cut(s)	125, 351, 2125
FatI	CATG	5 cut(s)	584, 1109, 1269, 2039, 2353
FauI	CCCGC	1 cut(s)	150
FauNDI	CATATG	1 cut(s)	2128
FbaI	TGATCA	1 cut(s)	1623
FblI	GTMKAC	1 cut(s)	646
FokI	GGATG	2 cut(s)	746, 1974
FriOI	GRGCYC	3 cut(s)	1448, 1715, 2036
FspBI	CTAG	4 cut(s)	228, 1007, 1557, 2330
GlaI	GCGC	4 cut(s)	43, 56, 58, 1574
GsaI	CCCAGC	1 cut(s)	1982
GsuI	CTGGAG	3 cut(s)	788, 1620, 1767
HaeII	RGCGCY	1 cut(s)	45
HaeIII	GGCC	9 cut(s)	34, 51, 308, 517, 1471, 1823, 2282, 2382, 2395
HapII	CCGG	6 cut(s)	36, 1449, 1468, 1511, 1991, 2051
HgaI	GACGC	2 cut(s)	348, 1373
HhaI	GCGC	4 cut(s)	44, 57, 59, 1575
Hin1II	CATG	5 cut(s)	588, 1113, 1273, 2043, 2357
Hin6I	GCGC	4 cut(s)	42, 55, 57, 1573
HinP1I	GCGC	4 cut(s)	42, 55, 57, 1573
HincII	GTYRAC	2 cut(s)	692, 1588
HindII	GTYRAC	2 cut(s)	692, 1588
HpaI	GTTAAC	1 cut(s)	1588
HpaII	CCGG	6 cut(s)	36, 1449, 1468, 1511, 1991, 2051
HphI	GGTGA	3 cut(s)	200, 1339, 2198
Hpy166II	GTNNAC	4 cut(s)	647, 692, 1069, 1588
Hpy8I	GTNNAC	4 cut(s)	647, 692, 1069, 1588
Hpy99I	CGWCG	1 cut(s)	1946
HpyAV	CCTTC	9 cut(s)	142, 197, 610, 724, 902, 1219, 1807, 2233, 2248
HpyCH4IV	ACGT	2 cut(s)	1606, 2088
HpyF10VI	GCNNNNNNNGC	8 cut(s)	48, 244, 379, 599, 997, 1421, 1489, 1516
HpyF3I	CTNAG	8 cut(s)	248, 369, 612, 840, 923, 954, 1375, 1809
HpySE526I	ACGT	2 cut(s)	1606, 2088
Hsp92II	CATG	5 cut(s)	588, 1113, 1273, 2043, 2357
HspAI	GCGC	4 cut(s)	42, 55, 57, 1573
Kpn2I	TCCGGA	1 cut(s)	1990
Ksp22I	TGATCA	1 cut(s)	1623
KspAI	GTTAAC	1 cut(s)	1588
Kzo9I	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
LguI	GCTCTTC	3 cut(s)	592, 1893, 2297
LmnI	GCTCC	7 cut(s)	562, 769, 844, 955, 1084, 1497, 1966
Lsp1109I	GCAGC	9 cut(s)	103, 244, 256, 1180, 1414, 1436, 1777, 1870, 2281
LweI	GCATC	2 cut(s)	337, 1802
MaeI	CTAG	4 cut(s)	228, 1007, 1557, 2330
MaeII	ACGT	2 cut(s)	1606, 2088
MaeIII	GTNAC	7 cut(s)	188, 209, 435, 530, 538, 2186, 2419
MalI	GATC	9 cut(s)	12, 234, 327, 427, 621, 810, 1555, 1561, 1625
MboI	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
MfeI	CAATTG	2 cut(s)	377, 1537
MflI	RGATCY	2 cut(s)	10, 808
MhlI	GDGCHC	3 cut(s)	1448, 1715, 2036
MlsI	TGGCCA	1 cut(s)	2395
MluNI	TGGCCA	1 cut(s)	2395
MlyI	GAGTC	3 cut(s)	695, 885, 1189
MmeI	TCCRAC	2 cut(s)	38, 2121
Mox20I	TGGCCA	1 cut(s)	2395
MroI	TCCGGA	1 cut(s)	1990
MroXI	GAANNNNTTC	1 cut(s)	463
MscI	TGGCCA	1 cut(s)	2395
MslI	CAYNNNNRTG	1 cut(s)	1954
Msp20I	TGGCCA	1 cut(s)	2395
MspA1I	CMGCKG	2 cut(s)	1427, 1790
MspCI	CTTAAG	3 cut(s)	569, 992, 2249
MspI	CCGG	6 cut(s)	36, 1449, 1468, 1511, 1991, 2051
MunI	CAATTG	2 cut(s)	377, 1537
Mva1269I	GAATGC	5 cut(s)	974, 993, 1417, 1494, 1521
MvaI	CCWGG	8 cut(s)	849, 896, 1389, 1473, 1485, 1494, 1521, 2335
MvnI	CGCG	2 cut(s)	57, 1575
MwoI	GCNNNNNNNGC	8 cut(s)	48, 244, 379, 599, 997, 1421, 1489, 1516
NciI	CCSGG	4 cut(s)	36, 1449, 1468, 1512
NcoI	CCATGG	1 cut(s)	584
NdeI	CATATG	1 cut(s)	2128
NdeII	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
NheI	GCTAGC	1 cut(s)	2329
NlaIII	CATG	5 cut(s)	588, 1113, 1273, 2043, 2357
NlaIV	GGNNCC	7 cut(s)	12, 141, 582, 771, 846, 1053, 1824
NmeAIII	GCCGAG	1 cut(s)	334
NmuCI	GTSAC	5 cut(s)	188, 209, 538, 2186, 2419
NspI	RCATGY	2 cut(s)	1113, 2357
NspV	TTCGAA	1 cut(s)	906
PauI	GCGCGC	1 cut(s)	55
PciSI	GCTCTTC	3 cut(s)	592, 1893, 2297
PctI	GAATGC	5 cut(s)	974, 993, 1417, 1494, 1521
PdmI	GAANNNNTTC	1 cut(s)	463
PflMI	CCANNNNNTGG	1 cut(s)	1305
PinAI	ACCGGT	1 cut(s)	2050
PleI	GAGTC	3 cut(s)	695, 884, 1188
PpsI	GAGTC	3 cut(s)	695, 884, 1188
PpuMI	RGGWCCY	2 cut(s)	139, 551
Psp1406I	AACGTT	1 cut(s)	2088
Psp5II	RGGWCCY	2 cut(s)	139, 551
Psp6I	CCWGG	8 cut(s)	847, 894, 1387, 1471, 1483, 1492, 1519, 2333
PspEI	GGTNACC	1 cut(s)	530
PspFI	CCCAGC	1 cut(s)	1978
PspGI	CCWGG	8 cut(s)	847, 894, 1387, 1471, 1483, 1492, 1519, 2333
PspN4I	GGNNCC	7 cut(s)	12, 141, 582, 771, 846, 1053, 1824
PspPI	GGNCC	8 cut(s)	33, 139, 499, 551, 1273, 1296, 1721, 1822
PspPPI	RGGWCCY	2 cut(s)	139, 551
PsuI	RGATCY	2 cut(s)	10, 808
PteI	GCGCGC	1 cut(s)	55
PvuII	CAGCTG	2 cut(s)	1427, 1790
RsaI	GTAC	4 cut(s)	1068, 1836, 1872, 2072
RsaNI	GTAC	4 cut(s)	1067, 1835, 1871, 2071
RseI	CAYNNNNRTG	1 cut(s)	1954
SapI	GCTCTTC	3 cut(s)	592, 1893, 2297
Sau3AI	GATC	9 cut(s)	10, 232, 325, 425, 619, 808, 1553, 1559, 1623
Sau96I	GGNCC	8 cut(s)	33, 139, 499, 551, 1273, 1296, 1721, 1822
SchI	GAGTC	3 cut(s)	695, 885, 1189
SduI	GDGCHC	3 cut(s)	1448, 1715, 2036
SfaNI	GCATC	2 cut(s)	337, 1802
SfcI	CTRYAG	2 cut(s)	1779, 2431
SfuI	TTCGAA	1 cut(s)	906
SinI	GGWCC	6 cut(s)	139, 499, 551, 1273, 1296, 1721
SmiMI	CAYNNNNRTG	1 cut(s)	1954
SmlI	CTYRAG	6 cut(s)	569, 683, 992, 1662, 2066, 2249
SmoI	CTYRAG	6 cut(s)	569, 683, 992, 1662, 2066, 2249
SsiI	CCGC	9 cut(s)	8, 39, 129, 143, 196, 575, 806, 1276, 1294
SspI	AATATT	1 cut(s)	1922
SspMI	CTAG	4 cut(s)	228, 1007, 1557, 2330
StyI	CCWWGG	1 cut(s)	584
TaiI	ACGT	2 cut(s)	1609, 2091
TaqI	TCGA	9 cut(s)	84, 220, 424, 595, 906, 1552, 1729, 1941, 2099
TaqII	GACCGA	1 cut(s)	2265
TatI	WGTACW	1 cut(s)	1066
TauI	GCSGC	1 cut(s)	42
TscAI	CASTG	4 cut(s)	642, 1675, 1933, 2403
TseFI	GTSAC	5 cut(s)	188, 209, 538, 2186, 2419
TseI	GCWGC	9 cut(s)	116, 244, 257, 1168, 1424, 1427, 1790, 1883, 2294
Tsp45I	GTSAC	5 cut(s)	188, 209, 538, 2186, 2419
TspDTI	ATGAA	4 cut(s)	1258, 1343, 2115, 2315
TspRI	CASTG	4 cut(s)	642, 1675, 1933, 2403
Van91I	CCANNNNNTGG	1 cut(s)	1305
Vha464I	CTTAAG	3 cut(s)	569, 992, 2249
VpaK11BI	GGWCC	6 cut(s)	139, 499, 551, 1273, 1296, 1721
XagI	CCTNNNNNAGG	2 cut(s)	2252, 2376
XapI	RAATTY	5 cut(s)	522, 1350, 1652, 1876, 2345
XbaI	TCTAGA	1 cut(s)	1556
XceI	RCATGY	2 cut(s)	1113, 2357
XmiI	GTMKAC	1 cut(s)	646
XmnI	GAANNNNTTC	1 cut(s)	463
XspI	CTAG	4 cut(s)	228, 1007, 1557, 2330

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.