MD02G1023900.v1.1

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010598
Physical Location & Seq
Reverse (-)
1777633 .. 1779996
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1023900.v1.1.491

Sequence Viewer

Length: 816 bp
ATGGCTCCGTCTTCGTCATCGACGCTTGCGAATCGGGACGCTTCTGCTTCAGCTGCTGAGGACGAAGCCGTCATGTCCGTGACCCGAGCTTTTGCGCAGGTTGCGTTGCTGCAGTTCCAGCCGGGCAAGTTCGATCAGCGCCTTACCGCCTTGTCTGAGTGCCTCAAGAGGAAGCCAAACGATCCCAAAACTTTTCATAATATCGGACTTGCGAATCGGGAAGGTTGTTCAGACCCTAAGAGGTTGCTTGATGTGCTTTATGATGTCAAGTTTCCCGAGGTTTCTAAGGATGATTTGAGTTTCAATAATGTGCCTCTGCTGGTTGAGGTATCCATTTACAAGTGGGGCACTTTTGCTGAATTCATAAGGCTTGCGTTCAGTCAACTCTCATGCCGTCTTTCTCATTTAGAGACCCTGAAGCTGGATATCAGGGGAGCGGTCTACAATCAGAATTATGCGTTTCCCGCGTTATCAAATCTAAAGCATTTGGAATTATTAGTGGATGCCAACTACTGTCTGTCCCTTGGTCGCTTAGCTTCTTTCATGAAGGCAGCTCCTGACTTGCGCAGCCTTGTTTTGGGGTTGGGTTTCGGAACCTCCAATGAGGACATGGCAATACTAGAGAAAGTTTCCAAACAACCCCATCACTGCCTCAAGGTGGTAGAAATAGCAGGGTATCGAGGTCACAAGTGTTGCGTAAAGCATGGCAGGTACTTGGGTGAAGAATGTTGTTGCACTAGAGAAGATTGTTATAAATCCTGTCCGGTTTTGGTGTCAGCCTACGGGAATCGAGATCAATTGCCTACTGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

272

Amino Acids

30.05

Weight (kDa)

7.51

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_At1g61320_AtMIF1 PF23622 99 - 233 8.7e-08 At1g61320/AtMIF1, LRR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AasI GACNNNNNNGTC 1 cut(s) 68
Acc16I TGCGCA 2 cut(s) 96, 566
Acc36I ACCTGC 2 cut(s) 88, 699
AccBSI CCGCTC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 441
AccII CGCG 1 cut(s) 467
AciI CCGC 3 cut(s) 147, 437, 465
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 1 cut(s) 359
AcuI CTGAAG 2 cut(s) 33, 437
AfaI GTAC 1 cut(s) 713
AfiI CCNNNNNNNGG 3 cut(s) 421, 577, 658
AgsI TTSAA 1 cut(s) 304
AluBI AGCT 5 cut(s) 53, 89, 421, 536, 554
AluI AGCT 5 cut(s) 53, 89, 421, 536, 554
Alw26I GTCTC 1 cut(s) 404
AlwI GGATC 1 cut(s) 176
AlwNI CAGNNNCTG 2 cut(s) 56, 557
Ama87I CYCGRG 2 cut(s) 84, 275
ApeKI GCWGC 4 cut(s) 53, 109, 551, 567
ApoI RAATTY 1 cut(s) 359
AspLEI GCGC 3 cut(s) 97, 141, 567
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 731
AvaI CYCGRG 2 cut(s) 84, 275
BaeGI GKGCMC 1 cut(s) 350
BaeI ACNNNNGTAYC 2 cut(s) 703, 736
BbsI GAAGAC 1 cut(s) 3
BbvCI CCTCAGC 1 cut(s) 57
BbvI GCAGC 4 cut(s) 40, 96, 563, 579
BccI CCATC 1 cut(s) 651
BceAI ACGGC 2 cut(s) 53, 378
BciVI GTATCC 1 cut(s) 340
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 2 cut(s) 620, 738
BfmI CTRYAG 1 cut(s) 110
BfoI RGCGCY 1 cut(s) 142
BfuAI ACCTGC 2 cut(s) 88, 699
BfuI GTATCC 1 cut(s) 340
BisI GCNGC 4 cut(s) 54, 110, 552, 568
BlpI GCTNAGC 1 cut(s) 532
BlsI GCNGC 4 cut(s) 55, 111, 553, 569
Bme1390I CCNGG 1 cut(s) 123
BmeT110I CYCGRG 2 cut(s) 84, 275
BmiI GGNNCC 2 cut(s) 6, 595
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 493
BpiI GAAGAC 1 cut(s) 3
Bpu10I CCTNAGC 1 cut(s) 57
Bpu1102I GCTNAGC 1 cut(s) 532
BpuEI CTTGAG 2 cut(s) 149, 638
BpuMI CCSGG 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 276, 523
BsaWI WCCGGW 1 cut(s) 763
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 577, 658
Bse1I ACTGG 1 cut(s) 811
BseDI CCNNGG 2 cut(s) 276, 523
BseGI GGATG 2 cut(s) 295, 508
BseLI CCNNNNNNNGG 3 cut(s) 421, 577, 658
BseMII CTCAG 2 cut(s) 48, 147
BseNI ACTGG 1 cut(s) 811
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 4 cut(s) 40, 96, 563, 579
Bsh1236I CGCG 1 cut(s) 467
BsiHKCI CYCGRG 2 cut(s) 84, 275
BsiSI CCGG 2 cut(s) 122, 764
BslFI GGGAC 2 cut(s) 50, 505
BslI CCNNNNNNNGG 3 cut(s) 421, 577, 658
BsmAI GTCTC 1 cut(s) 404
BsmFI GGGAC 2 cut(s) 50, 505
Bso31I GGTCTC 1 cut(s) 404
BsoBI CYCGRG 2 cut(s) 84, 275
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 3 cut(s) 133, 181, 793
Bsp1720I GCTNAGC 1 cut(s) 532
BspACI CCGC 3 cut(s) 147, 437, 465
BspCNI CTCAG 2 cut(s) 49, 148
BspFNI CGCG 1 cut(s) 467
BspHI TCATGA 1 cut(s) 543
BspLI GGNNCC 2 cut(s) 6, 595
BspMAI CTGCAG 1 cut(s) 114
BspMI ACCTGC 2 cut(s) 88, 699
BspPI GGATC 1 cut(s) 176
BspTNI GGTCTC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 437
BsrI ACTGG 1 cut(s) 811
BssECI CCNNGG 2 cut(s) 276, 523
BssMI GATC 3 cut(s) 133, 181, 793
BssT1I CCWWGG 1 cut(s) 523
Bst4CI ACNGT 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 27, 372
BstDEI CTNAG 5 cut(s) 57, 156, 237, 285, 532
BstF5I GGATG 2 cut(s) 295, 508
BstFNI CGCG 1 cut(s) 467
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 3 cut(s) 97, 141, 567
BstKTI GATC 3 cut(s) 136, 184, 796
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 3 cut(s) 133, 181, 793
BstMWI GCNNNNNNNGC 5 cut(s) 53, 101, 118, 253, 464
BstSCI CCNGG 1 cut(s) 121
BstSFI CTRYAG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 4 cut(s) 40, 96, 563, 579
BstV2I GAAGAC 1 cut(s) 3
BsuI GTATCC 1 cut(s) 340
BtsCI GGATG 2 cut(s) 295, 508
BtsI GCAGTG 1 cut(s) 646
BtsIMutI CAGTG 1 cut(s) 646
BveI ACCTGC 2 cut(s) 88, 699
Cac8I GCNNGC 2 cut(s) 27, 372
CaiI CAGNNNCTG 2 cut(s) 56, 557
CciI TCATGA 1 cut(s) 543
CfoI GCGC 3 cut(s) 97, 141, 567
CseI GACGC 2 cut(s) 31, 47
Csp6I GTAC 1 cut(s) 712
CviAII CATG 5 cut(s) 73, 390, 544, 610, 704
CviQI GTAC 1 cut(s) 712
DdeI CTNAG 5 cut(s) 57, 156, 237, 285, 532
DpnI GATC 3 cut(s) 135, 183, 795
DpnII GATC 3 cut(s) 133, 181, 793
DrdI GACNNNNNNGTC 1 cut(s) 68
DseDI GACNNNNNNGTC 1 cut(s) 68
Eco130I CCWWGG 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 404
Eco32I GATATC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 33, 437
Eco88I CYCGRG 2 cut(s) 84, 275
EcoRI GAATTC 1 cut(s) 359
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 523
FaeI CATG 5 cut(s) 76, 393, 547, 613, 707
FaqI GGGAC 2 cut(s) 50, 505
FatI CATG 5 cut(s) 72, 389, 543, 609, 703
FauI CCCGC 1 cut(s) 472
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 4 cut(s) 54, 110, 552, 568
FokI GGATG 2 cut(s) 302, 515
Fsp4HI GCNGC 4 cut(s) 54, 110, 552, 568
FspBI CTAG 2 cut(s) 620, 738
FspI TGCGCA 2 cut(s) 96, 566
GlaI GCGC 3 cut(s) 96, 140, 566
GluI GCNGC 4 cut(s) 54, 110, 552, 568
HaeII RGCGCY 1 cut(s) 142
HapII CCGG 2 cut(s) 122, 764
HgaI GACGC 2 cut(s) 31, 47
HhaI GCGC 3 cut(s) 97, 141, 567
Hin1II CATG 5 cut(s) 76, 393, 547, 613, 707
Hin6I GCGC 3 cut(s) 95, 139, 565
HinP1I GCGC 3 cut(s) 95, 139, 565
HincII GTYRAC 1 cut(s) 383
HindII GTYRAC 1 cut(s) 383
HinfI GANTC 3 cut(s) 31, 214, 787
HpaII CCGG 2 cut(s) 122, 764
HphI GGTGA 1 cut(s) 731
Hpy166II GTNNAC 2 cut(s) 383, 442
Hpy188I TCNGA 5 cut(s) 157, 206, 232, 450, 593
Hpy188III TCNNGA 7 cut(s) 35, 166, 218, 275, 544, 557, 791
Hpy8I GTNNAC 2 cut(s) 383, 442
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 215, 541
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4V TGCA 2 cut(s) 112, 735
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 101, 118, 253, 464
HpyF3I CTNAG 5 cut(s) 57, 156, 237, 285, 532
Hsp92II CATG 5 cut(s) 76, 393, 547, 613, 707
HspAI GCGC 3 cut(s) 95, 139, 565
Kzo9I GATC 3 cut(s) 133, 181, 793
LmnI GCTCC 3 cut(s) 10, 434, 559
Lsp1109I GCAGC 4 cut(s) 40, 96, 563, 579
LweI GCATC 1 cut(s) 493
MaeI CTAG 2 cut(s) 620, 738
MaeIII GTNAC 2 cut(s) 79, 683
MalI GATC 3 cut(s) 135, 183, 795
MbiI CCGCTC 1 cut(s) 437
MboI GATC 3 cut(s) 133, 181, 793
MboII GAAGA 3 cut(s) 3, 734, 755
MfeI CAATTG 1 cut(s) 797
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 4 cut(s) 359, 451, 491, 797
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 53
MspI CCGG 2 cut(s) 122, 764
MspR9I CCNGG 1 cut(s) 123
MunI CAATTG 1 cut(s) 797
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 5 cut(s) 53, 101, 118, 253, 464
NciI CCSGG 1 cut(s) 123
NdeII GATC 3 cut(s) 133, 181, 793
NlaIII CATG 5 cut(s) 76, 393, 547, 613, 707
NlaIV GGNNCC 2 cut(s) 6, 595
NmuCI GTSAC 2 cut(s) 79, 683
NsbI TGCGCA 2 cut(s) 96, 566
PagI TCATGA 1 cut(s) 543
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PfeI GAWTC 3 cut(s) 31, 214, 787
PkrI GCNGC 4 cut(s) 55, 111, 553, 569
PsiI TTATAA 1 cut(s) 753
PspN4I GGNNCC 2 cut(s) 6, 595
PstI CTGCAG 1 cut(s) 114
PstNI CAGNNNCTG 2 cut(s) 56, 557
PvuII CAGCTG 1 cut(s) 53
RsaI GTAC 1 cut(s) 713
RsaNI GTAC 1 cut(s) 712
RseI CAYNNNNRTG 1 cut(s) 77
SatI GCNGC 4 cut(s) 54, 110, 552, 568
Sau3AI GATC 3 cut(s) 133, 181, 793
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 1 cut(s) 493
SfcI CTRYAG 1 cut(s) 110
SmiMI CAYNNNNRTG 1 cut(s) 77
SmlI CTYRAG 2 cut(s) 164, 653
SmoI CTYRAG 2 cut(s) 164, 653
Sse9I AATT 4 cut(s) 359, 451, 491, 797
SsiI CCGC 3 cut(s) 147, 437, 465
SspMI CTAG 2 cut(s) 620, 738
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 523
TaaI ACNGT 1 cut(s) 515
TaqI TCGA 4 cut(s) 20, 132, 679, 790
TasI AATT 4 cut(s) 359, 451, 491, 797
TfiI GAWTC 3 cut(s) 31, 214, 787
TscAI CASTG 1 cut(s) 653
TseFI GTSAC 2 cut(s) 79, 683
TseI GCWGC 4 cut(s) 53, 109, 551, 567
Tsp45I GTSAC 2 cut(s) 79, 683
TspDTI ATGAA 4 cut(s) 185, 352, 532, 560
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 1 cut(s) 653
XapI RAATTY 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmiI GTMKAC 1 cut(s) 441
XspI CTAG 2 cut(s) 620, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.