RLG00000001463

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010598
Physical Location & Seq
Reverse (-)
15867371 .. 15872280
4910 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001463

Sequence Viewer

Length: 858 bp
ATGGCCTCCTTTATCTCCCTCATCAACGGCTTCGAACCGGGACGCAGGGCCGGTCCTGAGGCTGCCCTTGGGCAAGGCCGGGCCTGCCGGGATGACTCCTCACCTGAGGACGTCGCCGTTTTGTCCTCCACCAGGGCTTTTGCGCAGGACGCCTTGCTGCAATTCCAATTGGGGAAGTTCGATCAGTGCCTCACGGCCTTGCAGGAGCGCCTCAAGAGGAAGGCCGGCGATCCCAGAAACCTCAAAACAGCAATCTCAGCTCCAAAGCCTTCCTGGTTCTCCCCGAAAAGGGGGGATTTTAAATTGAACAACTTTCAAGATGGTGTTATCATCTGTGTTTATGGTTTGGCTTCTCGTGACCTCACCCATCTTTGCATCCTTGGGAAAGAGGTTGATTGGACTTGGGTTATCTATTTGGACATTTGCCACAGTCGAGTGTGGCTGGTTGGAGTCGGTGAGGCATCTTTGATAAGTCTTGCAGCTCCGTTCATTGATGACCATGCCCCTGCAGATCAGGAGGAAGAGAAAGTAGAAGAAGTACCTGAAATAACCCATAGAGTATACCTGGATATTGATATTGAGGAACAGCGCTTAGGTAGAATTGTGATTGGATTATACGGTCAGGTTGTACCAAAAACTGTTGAAAATTTCAGGGCATTGTGCATAGGAGAAAAAGGTAAAGGTGTTAATGGAAAACCACTCCACTATAAGGGAACGCCATTTCATCGTATCGTATCTGGGTTCATGATTCAAGGCGAAGATATCATTCATGGTGATGGGAAGGGGTATGAATCGATATATGGTGGCACCTTTGCTGATGAGAATTTCAAAGTGAAACATTCACATGCAGGTGTTTAA

Protein Analysis

286

Amino Acids

31.47

Weight (kDa)

6.0

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 191 - 284 6.2e-18 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 839
AatII GACGTC 1 cut(s) 114
Acc16I TGCGCA 1 cut(s) 144
Acc36I ACCTGC 1 cut(s) 839
AccB1I GGYRCC 1 cut(s) 806
AccI GTMKAC 1 cut(s) 561
AclWI GGATC 1 cut(s) 224
AcsI RAATTY 2 cut(s) 646, 823
AcyI GRCGYC 2 cut(s) 111, 150
AfaI GTAC 2 cut(s) 540, 630
AfeI AGCGCT 1 cut(s) 590
AfiI CCNNNNNNNGG 5 cut(s) 132, 288, 289, 290, 709
AgsI TTSAA 5 cut(s) 307, 317, 644, 752, 829
AjnI CCWGG 3 cut(s) 131, 272, 564
AluBI AGCT 2 cut(s) 260, 482
AluI AGCT 2 cut(s) 260, 482
AlwI GGATC 1 cut(s) 224
Aor51HI AGCGCT 1 cut(s) 590
AoxI GGCC 6 cut(s) 3, 48, 76, 81, 195, 222
ApeKI GCWGC 3 cut(s) 62, 157, 479
ApoI RAATTY 2 cut(s) 646, 823
AspLEI GCGC 3 cut(s) 145, 210, 591
AspS9I GGNCC 3 cut(s) 48, 53, 81
AsuC2I CCSGG 3 cut(s) 39, 80, 89
AsuHPI GGTGA 4 cut(s) 93, 355, 467, 785
AsuII TTCGAA 1 cut(s) 33
AvaII GGWCC 1 cut(s) 53
AxyI CCTNAGG 2 cut(s) 57, 105
BanI GGYRCC 1 cut(s) 806
BauI CACGAG 1 cut(s) 354
BbvI GCAGC 3 cut(s) 49, 144, 491
BccI CCATC 3 cut(s) 314, 375, 770
BceAI ACGGC 3 cut(s) 43, 101, 210
BciT130I CCWGG 3 cut(s) 133, 274, 566
BcnI CCSGG 3 cut(s) 39, 80, 89
BfmI CTRYAG 1 cut(s) 507
BfoI RGCGCY 2 cut(s) 211, 592
BfuAI ACCTGC 1 cut(s) 839
BisI GCNGC 3 cut(s) 63, 158, 480
BlsI GCNGC 3 cut(s) 64, 159, 481
Bme1390I CCNGG 6 cut(s) 39, 80, 89, 133, 274, 566
Bme18I GGWCC 1 cut(s) 53
BmgT120I GGNCC 3 cut(s) 48, 53, 81
BmiI GGNNCC 1 cut(s) 808
BmrFI CCNGG 6 cut(s) 39, 80, 89, 133, 274, 566
BmsI GCATC 2 cut(s) 384, 470
Bpu10I CCTNAGC 1 cut(s) 592
Bpu14I TTCGAA 1 cut(s) 33
BpuEI CTTGAG 1 cut(s) 197
BpuMI CCSGG 3 cut(s) 39, 80, 89
Bsa29I ATCGAT 1 cut(s) 794
BsaHI GRCGYC 2 cut(s) 111, 150
BsaJI CCNNGG 3 cut(s) 67, 132, 379
Bsc4I CCNNNNNNNGG 5 cut(s) 132, 288, 289, 290, 709
Bse118I RCCGGY 2 cut(s) 50, 224
Bse21I CCTNAGG 2 cut(s) 57, 105
BseBI CCWGG 3 cut(s) 133, 274, 566
BseCI ATCGAT 1 cut(s) 794
BseDI CCNNGG 3 cut(s) 67, 132, 379
BseGI GGATG 2 cut(s) 97, 375
BseLI CCNNNNNNNGG 5 cut(s) 132, 288, 289, 290, 709
BseMII CTCAG 3 cut(s) 48, 96, 270
BseRI GAGGAG 1 cut(s) 88
BseXI GCAGC 3 cut(s) 49, 144, 491
BshFI GGCC 6 cut(s) 5, 50, 78, 83, 197, 224
BshNI GGYRCC 1 cut(s) 806
BshVI ATCGAT 1 cut(s) 794
BsiSI CCGG 5 cut(s) 38, 51, 79, 88, 225
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 5 cut(s) 132, 288, 289, 290, 709
BsmFI GGGAC 1 cut(s) 54
BsnI GGCC 6 cut(s) 5, 50, 78, 83, 197, 224
Bsp119I TTCGAA 1 cut(s) 33
Bsp143I GATC 3 cut(s) 181, 229, 511
BspANI GGCC 6 cut(s) 5, 50, 78, 83, 197, 224
BspCNI CTCAG 3 cut(s) 49, 97, 269
BspDI ATCGAT 1 cut(s) 794
BspHI TCATGA 1 cut(s) 744
BspLI GGNNCC 1 cut(s) 808
BspMAI CTGCAG 1 cut(s) 511
BspMI ACCTGC 1 cut(s) 839
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 33
BspT107I GGYRCC 1 cut(s) 806
BsrFI RCCGGY 2 cut(s) 50, 224
BssAI RCCGGY 2 cut(s) 50, 224
BssECI CCNNGG 3 cut(s) 67, 132, 379
BssMI GATC 3 cut(s) 181, 229, 511
BssNAI GTATAC 1 cut(s) 562
BssNI GRCGYC 2 cut(s) 111, 150
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 67, 379
Bst1107I GTATAC 1 cut(s) 562
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 3 cut(s) 133, 274, 566
Bst4CI ACNGT 3 cut(s) 431, 620, 640
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 2 cut(s) 111, 150
BstBI TTCGAA 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 85, 226
BstDEI CTNAG 4 cut(s) 57, 105, 256, 592
BstENI CCTNNNNNAGG 1 cut(s) 130
BstF5I GGATG 2 cut(s) 97, 375
BstH2I RGCGCY 2 cut(s) 211, 592
BstHHI GCGC 3 cut(s) 145, 210, 591
BstKTI GATC 3 cut(s) 184, 232, 514
BstMBI GATC 3 cut(s) 181, 229, 511
BstMWI GCNNNNNNNGC 3 cut(s) 84, 149, 257
BstNI CCWGG 3 cut(s) 133, 274, 566
BstNSI RCATGY 1 cut(s) 848
BstSCI CCNGG 6 cut(s) 37, 78, 87, 131, 272, 564
BstSFI CTRYAG 1 cut(s) 507
BstV1I GCAGC 3 cut(s) 49, 144, 491
BstZ17I GTATAC 1 cut(s) 562
Bsu15I ATCGAT 1 cut(s) 794
Bsu36I CCTNAGG 2 cut(s) 57, 105
BsuRI GGCC 6 cut(s) 5, 50, 78, 83, 197, 224
BsuTUI ATCGAT 1 cut(s) 794
BtsCI GGATG 2 cut(s) 97, 375
BtsIMutI CAGTG 1 cut(s) 191
BveI ACCTGC 1 cut(s) 839
Cac8I GCNNGC 2 cut(s) 85, 226
CciI TCATGA 1 cut(s) 744
CfoI GCGC 3 cut(s) 145, 210, 591
Cfr10I RCCGGY 2 cut(s) 50, 224
Cfr13I GGNCC 3 cut(s) 48, 53, 81
ClaI ATCGAT 1 cut(s) 794
CseI GACGC 2 cut(s) 51, 158
Csp6I GTAC 2 cut(s) 539, 629
CviAII CATG 4 cut(s) 500, 745, 770, 845
CviQI GTAC 2 cut(s) 539, 629
DdeI CTNAG 4 cut(s) 57, 105, 256, 592
DpnI GATC 3 cut(s) 183, 231, 513
DpnII GATC 3 cut(s) 181, 229, 511
DraI TTTAAA 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 516
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 2 cut(s) 67, 379
Eco32I GATATC 1 cut(s) 763
Eco47I GGWCC 1 cut(s) 53
Eco47III AGCGCT 1 cut(s) 590
Eco81I CCTNAGG 2 cut(s) 57, 105
EcoNI CCTNNNNNAGG 1 cut(s) 130
EcoRII CCWGG 3 cut(s) 131, 272, 564
EcoRV GATATC 1 cut(s) 763
EcoT14I CCWWGG 2 cut(s) 67, 379
ErhI CCWWGG 2 cut(s) 67, 379
FaeI CATG 4 cut(s) 503, 748, 773, 848
FaqI GGGAC 1 cut(s) 54
FatI CATG 4 cut(s) 499, 744, 769, 844
FblI GTMKAC 1 cut(s) 561
Fnu4HI GCNGC 3 cut(s) 63, 158, 480
FokI GGATG 2 cut(s) 104, 362
Fsp4HI GCNGC 3 cut(s) 63, 158, 480
FspI TGCGCA 1 cut(s) 144
GlaI GCGC 3 cut(s) 144, 209, 590
GluI GCNGC 3 cut(s) 63, 158, 480
HaeII RGCGCY 2 cut(s) 211, 592
HaeIII GGCC 6 cut(s) 5, 50, 78, 83, 197, 224
HapII CCGG 5 cut(s) 38, 51, 79, 88, 225
HgaI GACGC 2 cut(s) 51, 158
HhaI GCGC 3 cut(s) 145, 210, 591
Hin1I GRCGYC 2 cut(s) 111, 150
Hin1II CATG 4 cut(s) 503, 748, 773, 848
Hin6I GCGC 3 cut(s) 143, 208, 589
HinP1I GCGC 3 cut(s) 143, 208, 589
HinfI GANTC 4 cut(s) 95, 450, 748, 791
HpaII CCGG 5 cut(s) 38, 51, 79, 88, 225
HphI GGTGA 4 cut(s) 93, 355, 467, 785
Hpy166II GTNNAC 1 cut(s) 562
Hpy188III TCNNGA 6 cut(s) 56, 214, 317, 356, 515, 745
Hpy8I GTNNAC 1 cut(s) 562
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 214, 279, 775
HpyCH4III ACNGT 3 cut(s) 431, 620, 640
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 7 cut(s) 160, 202, 375, 479, 509, 663, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 149, 257
HpyF3I CTNAG 4 cut(s) 57, 105, 256, 592
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 2 cut(s) 111, 150
Hsp92II CATG 4 cut(s) 503, 748, 773, 848
HspAI GCGC 3 cut(s) 143, 208, 589
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 181, 229, 511
LmnI GCTCC 3 cut(s) 205, 265, 487
Lsp1109I GCAGC 3 cut(s) 49, 144, 491
LweI GCATC 2 cut(s) 384, 470
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 356
MalI GATC 3 cut(s) 183, 231, 513
MboI GATC 3 cut(s) 181, 229, 511
MboII GAAGA 3 cut(s) 533, 545, 770
MfeI CAATTG 1 cut(s) 167
MluCI AATT 6 cut(s) 161, 167, 302, 600, 646, 823
MlyI GAGTC 2 cut(s) 89, 459
MmeI TCCRAC 1 cut(s) 427
MroNI GCCGGC 1 cut(s) 224
MseI TTAA 3 cut(s) 300, 687, 856
MslI CAYNNNNRTG 3 cut(s) 774, 843, 849
MspI CCGG 5 cut(s) 38, 51, 79, 88, 225
MspR9I CCNGG 6 cut(s) 39, 80, 89, 133, 274, 566
MunI CAATTG 1 cut(s) 167
MvaI CCWGG 3 cut(s) 133, 274, 566
MwoI GCNNNNNNNGC 3 cut(s) 84, 149, 257
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 3 cut(s) 39, 80, 89
NdeII GATC 3 cut(s) 181, 229, 511
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 4 cut(s) 503, 748, 773, 848
NlaIV GGNNCC 1 cut(s) 808
NmuCI GTSAC 1 cut(s) 356
NsbI TGCGCA 1 cut(s) 144
NspI RCATGY 1 cut(s) 848
NspV TTCGAA 1 cut(s) 33
PagI TCATGA 1 cut(s) 744
PaqCI CACCTGC 1 cut(s) 839
PdiI GCCGGC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 748, 791
PkrI GCNGC 3 cut(s) 64, 159, 481
PleI GAGTC 2 cut(s) 89, 458
PpsI GAGTC 2 cut(s) 89, 458
Psp6I CCWGG 3 cut(s) 131, 272, 564
PspGI CCWGG 3 cut(s) 131, 272, 564
PspN4I GGNNCC 1 cut(s) 808
PspPI GGNCC 3 cut(s) 48, 53, 81
PstI CTGCAG 1 cut(s) 511
RsaI GTAC 2 cut(s) 540, 630
RsaNI GTAC 2 cut(s) 539, 629
RseI CAYNNNNRTG 3 cut(s) 774, 843, 849
SaqAI TTAA 3 cut(s) 300, 687, 856
SatI GCNGC 3 cut(s) 63, 158, 480
Sau3AI GATC 3 cut(s) 181, 229, 511
Sau96I GGNCC 3 cut(s) 48, 53, 81
SchI GAGTC 2 cut(s) 89, 459
ScrFI CCNGG 6 cut(s) 39, 80, 89, 133, 274, 566
SfaNI GCATC 2 cut(s) 384, 470
SfcI CTRYAG 1 cut(s) 507
SfuI TTCGAA 1 cut(s) 33
SinI GGWCC 1 cut(s) 53
SmiMI CAYNNNNRTG 3 cut(s) 774, 843, 849
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 6 cut(s) 161, 167, 302, 600, 646, 823
StyD4I CCNGG 6 cut(s) 37, 78, 87, 131, 272, 564
StyI CCWWGG 2 cut(s) 67, 379
TaaI ACNGT 3 cut(s) 431, 620, 640
TaiI ACGT 1 cut(s) 114
TaqI TCGA 4 cut(s) 33, 180, 433, 794
TasI AATT 6 cut(s) 161, 167, 302, 600, 646, 823
TfiI GAWTC 2 cut(s) 748, 791
Tru1I TTAA 3 cut(s) 300, 687, 856
Tru9I TTAA 3 cut(s) 300, 687, 856
TscAI CASTG 1 cut(s) 191
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 3 cut(s) 62, 157, 479
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 5 cut(s) 478, 713, 733, 758, 804
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 53
XagI CCTNNNNNAGG 1 cut(s) 130
XapI RAATTY 2 cut(s) 646, 823
XceI RCATGY 1 cut(s) 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 561
ZraI GACGTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.