MD02G1031600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003565
Physical Location & Seq
Reverse (-)
2463247 .. 2465649
2403 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1031600.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGCAAGCTCTCTTTCTTTCACGCCTGTTTGTTCCTTCGCCACAAACAAACCAAGGGTTTTCATCGAAAGATCGATTGGCGGGAAGATTGTTCGGGTGAATGAAGTGTTTCGGAGCTCTAAAGCTGCAAACTTTCAGTCTTGGGAGGTCAAGGCTACAGACGGTACTCCAACAGCCAAAGTAAATAGCCTAGTCTGTTCAACATGTGAAGGAAACGGTGCAAAAGTGTGTAATCAATGCGAAGGTACTGGAGTTAATTCTGTAGACCACTTCAACGGACAGTTTAAGGCTGGTGGACTATGTTGGCTTTGCAGGGGGAAGAGGGAGATTTTGTGTGGAGATTGCAATGGTGCCGGGTTTTTGGGCGGCTTCATGAGCACACTTGACAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

13.77

Weight (kDa)

8.63

Isoelectric Point (pI)

26.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD2_CRD PF25436 62 - 129 1.4e-38 BSD2 cysteine rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 350
AccI GTMKAC 1 cut(s) 264
AciI CCGC 2 cut(s) 81, 366
AfaI GTAC 2 cut(s) 166, 247
AflIII ACRYGT 1 cut(s) 203
AgsI TTSAA 2 cut(s) 201, 274
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 4 cut(s) 9, 117, 125, 390
AluI AGCT 4 cut(s) 9, 117, 125, 390
Alw21I GWGCWC 2 cut(s) 119, 380
ApeKI GCWGC 1 cut(s) 125
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
Asp700I GAANNNNTTC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 1 cut(s) 109
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 119
Bbv12I GWGCWC 2 cut(s) 119, 380
BbvI GCAGC 1 cut(s) 112
BcnI CCSGG 1 cut(s) 355
BfaI CTAG 2 cut(s) 191, 391
BfmI CTRYAG 2 cut(s) 156, 261
BisI GCNGC 2 cut(s) 126, 367
BlsI GCNGC 2 cut(s) 127, 368
Bme1390I CCNGG 1 cut(s) 355
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 1 cut(s) 355
BpmI CTGGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 355
Bsa29I ATCGAT 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 317, 347
Bse1I ACTGG 1 cut(s) 253
Bse3DI GCAATG 1 cut(s) 352
BseCI ATCGAT 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 53
BseMI GCAATG 1 cut(s) 352
BseNI ACTGG 1 cut(s) 253
BseXI GCAGC 1 cut(s) 112
BshNI GGYRCC 1 cut(s) 350
BshVI ATCGAT 1 cut(s) 74
BsiHKAI GWGCWC 2 cut(s) 119, 380
BsiSI CCGG 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 119, 380
Bsp143I GATC 1 cut(s) 71
BspACI CCGC 2 cut(s) 81, 366
BspDI ATCGAT 1 cut(s) 74
BspHI TCATGA 1 cut(s) 372
BspLI GGNNCC 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 350
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 1 cut(s) 253
BssECI CCNNGG 1 cut(s) 53
BssMI GATC 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 53
Bst4CI ACNGT 3 cut(s) 164, 218, 282
Bst6I CTCTTC 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 74
BstMBI GATC 1 cut(s) 71
BstMWI GCNNNNNNNGC 1 cut(s) 375
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 1 cut(s) 353
BstSFI CTRYAG 2 cut(s) 156, 261
BstV1I GCAGC 1 cut(s) 112
Bsu15I ATCGAT 1 cut(s) 74
BsuTUI ATCGAT 1 cut(s) 74
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 372
ClaI ATCGAT 1 cut(s) 74
Csp6I GTAC 2 cut(s) 165, 246
CviAII CATG 2 cut(s) 204, 373
CviQI GTAC 2 cut(s) 165, 246
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 314
EarI CTCTTC 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 117
EcoICRI GAGCTC 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 53
EcoT38I GRGCYC 1 cut(s) 119
ErhI CCWWGG 1 cut(s) 53
FaeI CATG 2 cut(s) 207, 376
FaiI YATR 3 cut(s) 205, 301, 374
FatI CATG 2 cut(s) 203, 372
FauI CCCGC 1 cut(s) 74
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 2 cut(s) 126, 367
FriOI GRGCYC 1 cut(s) 119
Fsp4HI GCNGC 2 cut(s) 126, 367
FspBI CTAG 2 cut(s) 191, 391
GluI GCNGC 2 cut(s) 126, 367
GsuI CTGGAG 1 cut(s) 270
HapII CCGG 1 cut(s) 354
Hin1II CATG 2 cut(s) 207, 376
HpaII CCGG 1 cut(s) 354
HphI GGTGA 1 cut(s) 109
Hpy166II GTNNAC 2 cut(s) 265, 296
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 2 cut(s) 265, 296
HpyAV CCTTC 3 cut(s) 46, 203, 236
HpyCH4III ACNGT 3 cut(s) 164, 218, 282
HpyCH4V TGCA 4 cut(s) 128, 221, 312, 345
HpyF10VI GCNNNNNNNGC 1 cut(s) 375
Hsp92II CATG 2 cut(s) 207, 376
Kzo9I GATC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 114
LpnPI CCDG 5 cut(s) 39, 234, 276, 298, 367
Lsp1109I GCAGC 1 cut(s) 112
MaeI CTAG 2 cut(s) 191, 391
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MboII GAAGA 2 cut(s) 97, 331
MhlI GDGCHC 2 cut(s) 119, 380
MluCI AATT 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 194
MnlI CCTC 2 cut(s) 139, 315
MroXI GAANNNNTTC 1 cut(s) 108
MseI TTAA 2 cut(s) 255, 285
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 375
NciI CCSGG 1 cut(s) 355
NdeII GATC 1 cut(s) 71
NlaIII CATG 2 cut(s) 207, 376
NlaIV GGNNCC 1 cut(s) 352
NspI RCATGY 1 cut(s) 207
PagI TCATGA 1 cut(s) 372
PciI ACATGT 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 108
PkrI GCNGC 2 cut(s) 127, 368
PscI ACATGT 1 cut(s) 203
Psp124BI GAGCTC 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 352
RsaI GTAC 2 cut(s) 166, 247
RsaNI GTAC 2 cut(s) 165, 246
SacI GAGCTC 1 cut(s) 119
SaqAI TTAA 2 cut(s) 255, 285
SatI GCNGC 2 cut(s) 126, 367
Sau3AI GATC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 355
SduI GDGCHC 2 cut(s) 119, 380
SetI ASST 6 cut(s) 11, 119, 127, 150, 247, 392
SfcI CTRYAG 2 cut(s) 156, 261
Sse9I AATT 1 cut(s) 256
SsiI CCGC 2 cut(s) 81, 366
SspMI CTAG 2 cut(s) 191, 391
SstI GAGCTC 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 353
StyI CCWWGG 1 cut(s) 53
TaaI ACNGT 3 cut(s) 164, 218, 282
TaqI TCGA 2 cut(s) 66, 74
TasI AATT 1 cut(s) 256
TauI GCSGC 1 cut(s) 369
Tru1I TTAA 2 cut(s) 255, 285
Tru9I TTAA 2 cut(s) 255, 285
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 52, 117, 361
TspGWI ACGGA 1 cut(s) 291
XceI RCATGY 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 108
XspI CTAG 2 cut(s) 191, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.