MD15G1173100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003565
Physical Location & Seq
Reverse (-)
13470104 .. 13471929
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1173100.v1.1.491

Sequence Viewer

Length: 396 bp
ATGGCAACCTCTCTTTCTTTCACGCCTCTTTGTTCCTTCAATTCCATAAACAAACCAAGGGTTTATATCGAAAATTCGACTGGCGGGAAGGTTATTCGGGTGAATGAAGTGTTTCGGAACTCGAAAGCTGCAAGCTTTCAGTCTTGGGAGGTCAAGGCCACAGATGGTACTCCAACGACCAAAGTGAATAGCATAGTTTGTTCTACATGTGAGGGAAATGGTGCGAAAGTGTGTAGTCAATGTGAAGGTACGGGACTAAATTCTGTAGACCACTTCAACGGACAGTTTAAAGCCGGTGGAGTATGTTGGCTTTGCAGGGGGAAGAGGGAGATTTTGTGTGGAGATTGCAATGGTGCCGGGTTTTTGGGCGGCTTCATGAGCACGCATGACGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

13.91

Weight (kDa)

8.4

Isoelectric Point (pI)

17.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD2_CRD PF25436 63 - 130 1.2e-38 BSD2 cysteine rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 267
AciI CCGC 2 cut(s) 84, 369
AcsI RAATTY 2 cut(s) 73, 259
AcyI GRCGYC 1 cut(s) 390
AfaI GTAC 2 cut(s) 169, 250
AflIII ACRYGT 1 cut(s) 206
AgsI TTSAA 2 cut(s) 40, 277
AluBI AGCT 2 cut(s) 128, 135
AluI AGCT 2 cut(s) 128, 135
Alw21I GWGCWC 1 cut(s) 383
AoxI GGCC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 128
ApoI RAATTY 2 cut(s) 73, 259
Asp700I GAANNNNTTC 1 cut(s) 111
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 112
BanI GGYRCC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 1 cut(s) 115
BccI CCATC 1 cut(s) 158
BcnI CCSGG 1 cut(s) 358
BfaI CTAG 1 cut(s) 394
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 2 cut(s) 129, 370
BlsI GCNGC 2 cut(s) 130, 371
Bme1390I CCNGG 1 cut(s) 358
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 358
BpuMI CCSGG 1 cut(s) 358
BsaHI GRCGYC 1 cut(s) 390
BsaJI CCNNGG 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 355
BseDI CCNNGG 1 cut(s) 56
BseMI GCAATG 1 cut(s) 355
BseNI ACTGG 1 cut(s) 85
BseXI GCAGC 1 cut(s) 115
BshFI GGCC 1 cut(s) 158
BshNI GGYRCC 1 cut(s) 353
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 2 cut(s) 294, 357
BslFI GGGAC 1 cut(s) 267
BsmFI GGGAC 1 cut(s) 267
BsnI GGCC 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 383
BspACI CCGC 2 cut(s) 84, 369
BspANI GGCC 1 cut(s) 158
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 1 cut(s) 85
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 1 cut(s) 56
BssNI GRCGYC 1 cut(s) 390
BssT1I CCWWGG 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 285
Bst6I CTCTTC 1 cut(s) 317
BstACI GRCGYC 1 cut(s) 390
BstC8I GCNNGC 2 cut(s) 133, 383
BstMWI GCNNNNNNNGC 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 356
BstSFI CTRYAG 1 cut(s) 264
BstV1I GCAGC 1 cut(s) 115
BsuRI GGCC 1 cut(s) 158
Cac8I GCNNGC 2 cut(s) 133, 383
CciI TCATGA 1 cut(s) 375
Cfr10I RCCGGY 1 cut(s) 293
Csp6I GTAC 2 cut(s) 168, 249
CviAII CATG 3 cut(s) 207, 376, 386
CviJI RGCY 6 cut(s) 128, 135, 158, 293, 310, 372
CviKI_1 RGCY 6 cut(s) 128, 135, 158, 293, 310, 372
CviQI GTAC 2 cut(s) 168, 249
DraI TTTAAA 1 cut(s) 289
Eam1104I CTCTTC 1 cut(s) 317
EarI CTCTTC 1 cut(s) 317
Eco130I CCWWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 3 cut(s) 210, 379, 389
FaiI YATR 7 cut(s) 47, 66, 194, 208, 304, 377, 387
FaqI GGGAC 1 cut(s) 267
FatI CATG 3 cut(s) 206, 375, 385
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 2 cut(s) 129, 370
Fsp4HI GCNGC 2 cut(s) 129, 370
FspBI CTAG 1 cut(s) 394
GluI GCNGC 2 cut(s) 129, 370
HaeIII GGCC 1 cut(s) 158
HapII CCGG 2 cut(s) 294, 357
Hin1I GRCGYC 1 cut(s) 390
Hin1II CATG 3 cut(s) 210, 379, 389
HindIII AAGCTT 1 cut(s) 133
HpaII CCGG 2 cut(s) 294, 357
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 1 cut(s) 117
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 1 cut(s) 268
HpyAV CCTTC 3 cut(s) 46, 82, 239
HpyCH4III ACNGT 1 cut(s) 285
HpyCH4V TGCA 3 cut(s) 131, 315, 348
HpyF10VI GCNNNNNNNGC 1 cut(s) 378
Hsp92I GRCGYC 1 cut(s) 390
Hsp92II CATG 3 cut(s) 210, 379, 389
LpnPI CCDG 4 cut(s) 66, 301, 307, 370
Lsp1109I GCAGC 1 cut(s) 115
MaeI CTAG 1 cut(s) 394
MboII GAAGA 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 3 cut(s) 40, 73, 259
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 5 cut(s) 19, 36, 142, 205, 318
MroXI GAANNNNTTC 1 cut(s) 111
MseI TTAA 1 cut(s) 288
MspI CCGG 2 cut(s) 294, 357
MspR9I CCNGG 1 cut(s) 358
MwoI GCNNNNNNNGC 1 cut(s) 378
NciI CCSGG 1 cut(s) 358
NlaIII CATG 3 cut(s) 210, 379, 389
NlaIV GGNNCC 1 cut(s) 355
NspI RCATGY 1 cut(s) 210
PagI TCATGA 1 cut(s) 375
PciI ACATGT 1 cut(s) 206
PdmI GAANNNNTTC 1 cut(s) 111
PkrI GCNGC 2 cut(s) 130, 371
PscI ACATGT 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 355
RsaI GTAC 2 cut(s) 169, 250
RsaNI GTAC 2 cut(s) 168, 249
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 2 cut(s) 129, 370
ScrFI CCNGG 1 cut(s) 358
SduI GDGCHC 1 cut(s) 383
SetI ASST 6 cut(s) 11, 93, 130, 137, 153, 250
SfcI CTRYAG 1 cut(s) 264
Sse9I AATT 3 cut(s) 40, 73, 259
SsiI CCGC 2 cut(s) 84, 369
SspMI CTAG 1 cut(s) 394
StyD4I CCNGG 1 cut(s) 356
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 1 cut(s) 285
TaqI TCGA 3 cut(s) 69, 77, 122
TasI AATT 3 cut(s) 40, 73, 259
TauI GCSGC 1 cut(s) 372
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseI GCWGC 1 cut(s) 128
TspDTI ATGAA 2 cut(s) 120, 364
TspGWI ACGGA 1 cut(s) 294
XapI RAATTY 2 cut(s) 73, 259
XceI RCATGY 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 111
XspI CTAG 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.