RLG00000011539

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003565
Physical Location & Seq
Forward (+)
10389224 .. 10391050
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011539

Sequence Viewer

Length: 615 bp
ATGAGATTTGAGAGAAGGAGAGGGAAAGGGAGAAAGAGGGTTGATCTGGGTGAGATTTTGTTGAAGATGGCAATGGAATTGGAATCCAGAGGGGAGCGAATTAGCAGGCGGACAGGGGTGGAAGTGCTGATGCCAGCGACAATTGGGAGGGCGGACAGTAGTCAGACTGGTGGTGCCAGGCTGCTACCTAGCTCCTCCCTCATTCTCCATTCCTCAATAATGGCCTATGCTCTCTACTCAGCTCCTTTAAGTTCCTTCAAGGCCACACCAGCACCAGGTATTCATAGCAGTAACCAGAAGCTCAGTCATATAAACTACTTCGTAAATAGCTCTCCAGCTGCTAAAGTGTTCCATGTGAAGGCTAAGGCTGCCAAAAGTGATGAAAGCACAAAGCGAAACAGCATGGTTTGTTCTGATTGTGAGGGAAATGGTGCAGTTCTCTGCTCTCAATGCAAGGGCAGTGGAGTAAACTCCGATGATCTGTTTAGTGGACAGTTTAAAGCTGGGGATTCATGCTGGCTTTGCGGTGGCAGAAAGGAGATGTTGTGCGGAAATTGCAATGGAGCCGGCTTTCTTGGAGGTTTCATGAGCACTTCTGATACTACGTCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

21.77

Weight (kDa)

9.3

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD2_CRD PF25436 133 - 200 4.4e-37 BSD2 cysteine rich domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 173
AciI CCGC 4 cut(s) 109, 152, 525, 549
AfiI CCNNNNNNNGG 2 cut(s) 275, 358
AgsI TTSAA 2 cut(s) 64, 259
AjnI CCWGG 2 cut(s) 176, 274
AluBI AGCT 6 cut(s) 192, 242, 301, 330, 338, 503
AluI AGCT 6 cut(s) 192, 242, 301, 330, 338, 503
Alw21I GWGCWC 1 cut(s) 593
AoxI GGCC 2 cut(s) 222, 261
ApeKI GCWGC 3 cut(s) 181, 338, 368
AsuHPI GGTGA 1 cut(s) 62
BanI GGYRCC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 593
BbvI GCAGC 3 cut(s) 168, 325, 355
BccI CCATC 1 cut(s) 61
BciT130I CCWGG 2 cut(s) 178, 276
BfaI CTAG 1 cut(s) 189
BisI GCNGC 3 cut(s) 182, 339, 369
BlsI GCNGC 3 cut(s) 183, 340, 370
Bme1390I CCNGG 2 cut(s) 178, 276
BmiI GGNNCC 2 cut(s) 175, 565
BmrFI CCNGG 2 cut(s) 178, 276
BmsI GCATC 1 cut(s) 120
BoxI GACNNNNGTC 1 cut(s) 159
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 530, 560
Bsc4I CCNNNNNNNGG 2 cut(s) 275, 358
Bse118I RCCGGY 1 cut(s) 566
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 2 cut(s) 78, 565
BseBI CCWGG 2 cut(s) 178, 276
BseLI CCNNNNNNNGG 2 cut(s) 275, 358
BseMI GCAATG 2 cut(s) 78, 565
BseMII CTCAG 2 cut(s) 252, 316
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 184
BseXI GCAGC 3 cut(s) 168, 325, 355
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 2 cut(s) 224, 263
BshNI GGYRCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 593
BsiSI CCGG 1 cut(s) 567
BslI CCNNNNNNNGG 2 cut(s) 275, 358
BsnI GGCC 2 cut(s) 224, 263
Bsp1286I GDGCHC 1 cut(s) 593
Bsp143I GATC 2 cut(s) 43, 478
BspACI CCGC 4 cut(s) 109, 152, 525, 549
BspANI GGCC 2 cut(s) 224, 263
BspCNI CTCAG 2 cut(s) 251, 315
BspHI TCATGA 1 cut(s) 585
BspLI GGNNCC 2 cut(s) 175, 565
BspT107I GGYRCC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 78, 565
BsrFI RCCGGY 1 cut(s) 566
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 566
BssMI GATC 2 cut(s) 43, 478
Bst2UI CCWGG 2 cut(s) 178, 276
Bst4CI ACNGT 2 cut(s) 158, 495
BstC8I GCNNGC 4 cut(s) 107, 135, 518, 568
BstDEI CTNAG 3 cut(s) 238, 302, 363
BstKTI GATC 2 cut(s) 46, 481
BstMBI GATC 2 cut(s) 43, 478
BstMWI GCNNNNNNNGC 5 cut(s) 269, 368, 450, 522, 555
BstNI CCWGG 2 cut(s) 178, 276
BstPAI GACNNNNGTC 1 cut(s) 159
BstSCI CCNGG 2 cut(s) 176, 274
BstV1I GCAGC 3 cut(s) 168, 325, 355
BsuRI GGCC 2 cut(s) 224, 263
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 1 cut(s) 466
Cac8I GCNNGC 4 cut(s) 107, 135, 518, 568
CciI TCATGA 1 cut(s) 585
Cfr10I RCCGGY 1 cut(s) 566
CsiI ACCWGGT 1 cut(s) 274
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 4 cut(s) 353, 403, 513, 586
DdeI CTNAG 3 cut(s) 238, 302, 363
DpnI GATC 2 cut(s) 45, 480
DpnII GATC 2 cut(s) 43, 478
DraI TTTAAA 1 cut(s) 499
EciI GGCGGA 2 cut(s) 124, 167
EcoRII CCWGG 2 cut(s) 176, 274
FaeI CATG 4 cut(s) 356, 406, 516, 589
FaiI YATR 8 cut(s) 228, 285, 309, 311, 354, 404, 514, 587
FatI CATG 4 cut(s) 352, 402, 512, 585
Fnu4HI GCNGC 3 cut(s) 182, 339, 369
Fsp4HI GCNGC 3 cut(s) 182, 339, 369
FspBI CTAG 1 cut(s) 189
GluI GCNGC 3 cut(s) 182, 339, 369
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 2 cut(s) 224, 263
HapII CCGG 1 cut(s) 567
Hin1II CATG 4 cut(s) 356, 406, 516, 589
HinfI GANTC 2 cut(s) 83, 509
HpaII CCGG 1 cut(s) 567
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 2 cut(s) 469, 491
Hpy188I TCNGA 5 cut(s) 165, 415, 475, 598, 610
Hpy188III TCNNGA 2 cut(s) 87, 586
Hpy8I GTNNAC 2 cut(s) 469, 491
HpyAV CCTTC 3 cut(s) 9, 265, 352
HpyCH4III ACNGT 2 cut(s) 158, 495
HpyCH4IV ACGT 1 cut(s) 605
HpyCH4V TGCA 3 cut(s) 434, 453, 558
HpyF10VI GCNNNNNNNGC 5 cut(s) 269, 368, 450, 522, 555
HpyF3I CTNAG 3 cut(s) 238, 302, 363
HpySE526I ACGT 1 cut(s) 605
Hsp92II CATG 4 cut(s) 356, 406, 516, 589
KroI GCCGGC 1 cut(s) 566
KroNI GCCGGC 1 cut(s) 568
Kzo9I GATC 2 cut(s) 43, 478
LmnI GCTCC 4 cut(s) 94, 197, 247, 563
Lsp1109I GCAGC 3 cut(s) 168, 325, 355
LweI GCATC 1 cut(s) 120
MabI ACCWGGT 1 cut(s) 274
MaeI CTAG 1 cut(s) 189
MaeII ACGT 1 cut(s) 605
MaeIII GTNAC 1 cut(s) 290
MalI GATC 2 cut(s) 45, 480
MboI GATC 2 cut(s) 43, 478
MboII GAAGA 1 cut(s) 76
MfeI CAATTG 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 593
MluCI AATT 4 cut(s) 77, 99, 141, 553
MnlI CCTC 9 cut(s) 14, 30, 83, 141, 205, 209, 223, 415, 572
MroNI GCCGGC 1 cut(s) 566
MseI TTAA 2 cut(s) 248, 498
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 567
MspR9I CCNGG 2 cut(s) 178, 276
MunI CAATTG 1 cut(s) 141
MvaI CCWGG 2 cut(s) 178, 276
MwoI GCNNNNNNNGC 5 cut(s) 269, 368, 450, 522, 555
NaeI GCCGGC 1 cut(s) 568
NdeII GATC 2 cut(s) 43, 478
NgoMIV GCCGGC 1 cut(s) 566
NlaIII CATG 4 cut(s) 356, 406, 516, 589
NlaIV GGNNCC 2 cut(s) 175, 565
PagI TCATGA 1 cut(s) 585
PdiI GCCGGC 1 cut(s) 568
PfeI GAWTC 2 cut(s) 83, 509
PkrI GCNGC 3 cut(s) 183, 340, 370
PshAI GACNNNNGTC 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 176, 274
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 2 cut(s) 176, 274
PspN4I GGNNCC 2 cut(s) 175, 565
PvuII CAGCTG 1 cut(s) 338
SaqAI TTAA 2 cut(s) 248, 498
SatI GCNGC 3 cut(s) 182, 339, 369
Sau3AI GATC 2 cut(s) 43, 478
ScrFI CCNGG 2 cut(s) 178, 276
SduI GDGCHC 1 cut(s) 593
SexAI ACCWGGT 1 cut(s) 274
SfaNI GCATC 1 cut(s) 120
Sse9I AATT 4 cut(s) 77, 99, 141, 553
SsiI CCGC 4 cut(s) 109, 152, 525, 549
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 176, 274
TaaI ACNGT 2 cut(s) 158, 495
TaiI ACGT 1 cut(s) 608
TasI AATT 4 cut(s) 77, 99, 141, 553
TfiI GAWTC 2 cut(s) 83, 509
Tru1I TTAA 2 cut(s) 248, 498
Tru9I TTAA 2 cut(s) 248, 498
TscAI CASTG 1 cut(s) 466
TseI GCWGC 3 cut(s) 181, 338, 368
TspDTI ATGAA 4 cut(s) 272, 396, 501, 574
TspRI CASTG 1 cut(s) 466
XspI CTAG 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.