MD02G1034400.v1.1

Sel1-like repeats.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007196
Physical Location & Seq
Reverse (-)
2635529 .. 2638668
3140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1034400.v1.1.491

Sequence Viewer

Length: 822 bp
ATGCAGAGGCAGCTGCTGAAGAAGCTCAGTCTCCGACCTCCCAATGGACTCACCCAATTCAATCAGCGGAGAGAGATGCACAGCAGGAACAAGAAGGCGATGGAGTTGATAGCAAAAGGCTGGAGCGCCCTCAAGGAAGTCGACCGAGTCATCGATTACTGCGAGCTCAACGATCGCCGCCTCATCCCTCTTTTACGGGGAGCGAAGGAGAATTTTGAGTTGGCACTGGAGGCTGACAATTCGAATACCCATGCCAGGTATTGGTTGTCCAAATTGCATTTGAAATACCATGTCCCGGGAGCCTGCAAAGCTATAGGAGCTGCTTTGTTAGTTGAAGCGGCAGATATGGGTGATCCGGATGCACAGTACGAACTGGGTTGTCGTCTTCGAGTTGATAATGAATACGTCCATTCGGATCAGCAAGCTTTCCATTATATAGAGAAGGCTGTCGACCAGTTGCATCCAGGTGCTCTTTACCTTCTTGGTTCTGTGTATCTGACGGGAGACTGCGTGAAAAAAGATATTGCCTCTGCAATGTGGTGTTTCTATAGGGCATCGGATAAGGGACATCCCGGTGCAGCTATAGCATTTGGATCTCTTCTTCTTAGAGGTGCTGAAATCCCCGAATCCGTGACGAAGTTCTCTTCAAAGAGGGGTCCTTTGGCCAGAAAGGAAAGCGTAACAGATCCCATAGAGATGGCAAGAGAACGATTCCAGATTGCTGCGAAAGCAGGATGTGATCTTGGACTTAAATGGTTGGAAAGACTTGAGGAGGAAGAGAAACATCTTTTGACCGGATCTGAAATGGCTGAACAGGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sel1

Protein Analysis

274

Amino Acids

30.74

Weight (kDa)

7.65

Isoelectric Point (pI)

29.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sel1 PF08238 157 - 190 4.3e-06 Sel1 repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 261
AccI GTMKAC 2 cut(s) 141, 450
AccIII TCCGGA 1 cut(s) 355
AciI CCGC 3 cut(s) 67, 178, 338
AclWI GGATC 5 cut(s) 347, 423, 601, 680, 805
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 38
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 4 cut(s) 44, 255, 261, 295
AgsI TTSAA 4 cut(s) 61, 283, 335, 648
AjnI CCWGG 2 cut(s) 254, 463
AjuI GAANNNNNNNTTGG 2 cut(s) 203, 235
AluBI AGCT 7 cut(s) 13, 25, 166, 311, 320, 425, 581
AluI AGCT 7 cut(s) 13, 25, 166, 311, 320, 425, 581
Alw21I GWGCWC 2 cut(s) 168, 472
Alw26I GTCTC 2 cut(s) 35, 498
AlwI GGATC 5 cut(s) 347, 423, 601, 680, 805
AlwNI CAGNNNCTG 1 cut(s) 16
Ama87I CYCGRG 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 355
AoxI GGCC 1 cut(s) 663
ApeKI GCWGC 5 cut(s) 10, 13, 320, 578, 722
ApoI RAATTY 1 cut(s) 211
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 656
AsuC2I CCSGG 3 cut(s) 296, 297, 573
AsuHPI GGTGA 2 cut(s) 43, 362
AsuII TTCGAA 1 cut(s) 242
AvaI CYCGRG 1 cut(s) 295
AvaII GGWCC 1 cut(s) 656
BalI TGGCCA 1 cut(s) 665
BanII GRGCYC 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 377
Bbv12I GWGCWC 2 cut(s) 168, 472
BbvI GCAGC 4 cut(s) 22, 307, 590, 709
BccI CCATC 2 cut(s) 94, 691
BciT130I CCWGG 2 cut(s) 256, 465
BcnI CCSGG 3 cut(s) 296, 297, 573
BcoDI GTCTC 2 cut(s) 35, 498
BfmI CTRYAG 3 cut(s) 312, 547, 582
BfoI RGCGCY 1 cut(s) 129
BisI GCNGC 7 cut(s) 11, 14, 178, 321, 339, 579, 723
BlsI GCNGC 7 cut(s) 12, 15, 179, 322, 340, 580, 724
Bme1390I CCNGG 5 cut(s) 256, 296, 297, 465, 573
Bme18I GGWCC 1 cut(s) 656
BmeT110I CYCGRG 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 656
BmiI GGNNCC 2 cut(s) 301, 657
BmrFI CCNGG 5 cut(s) 256, 296, 297, 465, 573
BmrI ACTGGG 1 cut(s) 383
BmsI GCATC 4 cut(s) 66, 349, 469, 563
BmuI ACTGGG 1 cut(s) 383
BpiI GAAGAC 1 cut(s) 377
BpmI CTGGAG 2 cut(s) 142, 248
Bpu14I TTCGAA 1 cut(s) 242
BpuEI CTTGAG 2 cut(s) 116, 788
BpuMI CCSGG 3 cut(s) 296, 297, 573
Bsa29I ATCGAT 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 2 cut(s) 355, 794
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 255, 261, 295
Bse1I ACTGG 3 cut(s) 231, 378, 454
Bse3DI GCAATG 1 cut(s) 540
BseAI TCCGGA 1 cut(s) 355
BseBI CCWGG 2 cut(s) 256, 465
BseCI ATCGAT 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 5 cut(s) 183, 364, 460, 568, 740
BseLI CCNNNNNNNGG 4 cut(s) 44, 255, 261, 295
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 1 cut(s) 40
BseNI ACTGG 3 cut(s) 231, 378, 454
BseRI GAGGAG 1 cut(s) 785
BseXI GCAGC 4 cut(s) 22, 307, 590, 709
BsgI GTGCAG 1 cut(s) 597
Bsh1285I CGRYCG 2 cut(s) 145, 175
BshFI GGCC 1 cut(s) 665
BshVI ATCGAT 1 cut(s) 153
BsiEI CGRYCG 2 cut(s) 145, 175
BsiHKAI GWGCWC 2 cut(s) 168, 472
BsiHKCI CYCGRG 1 cut(s) 295
BsiSI CCGG 4 cut(s) 296, 356, 573, 795
BslFI GGGAC 2 cut(s) 278, 579
BslI CCNNNNNNNGG 4 cut(s) 44, 255, 261, 295
BsmAI GTCTC 2 cut(s) 35, 498
BsmFI GGGAC 2 cut(s) 278, 579
BsnI GGCC 1 cut(s) 665
BsoBI CYCGRG 1 cut(s) 295
Bsp119I TTCGAA 1 cut(s) 242
Bsp1286I GDGCHC 2 cut(s) 168, 472
Bsp13I TCCGGA 1 cut(s) 355
Bsp143I GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
BspACI CCGC 3 cut(s) 67, 178, 338
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 1 cut(s) 39
BspDI ATCGAT 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 355
BspLI GGNNCC 2 cut(s) 301, 657
BspPI GGATC 5 cut(s) 347, 423, 601, 680, 805
BspT104I TTCGAA 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 3 cut(s) 231, 378, 454
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
Bst2UI CCWGG 2 cut(s) 256, 465
Bst4CI ACNGT 1 cut(s) 366
Bst6I CTCTTC 3 cut(s) 603, 649, 771
BstBI TTCGAA 1 cut(s) 242
BstC8I GCNNGC 3 cut(s) 164, 304, 423
BstDEI CTNAG 2 cut(s) 26, 605
BstF5I GGATG 5 cut(s) 183, 364, 460, 568, 740
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 7 cut(s) 175, 355, 418, 596, 688, 742, 800
BstMAI GTCTC 2 cut(s) 35, 498
BstMBI GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
BstMCI CGRYCG 2 cut(s) 145, 175
BstMWI GCNNNNNNNGC 7 cut(s) 10, 22, 230, 308, 317, 584, 728
BstNI CCWGG 2 cut(s) 256, 465
BstSCI CCNGG 5 cut(s) 254, 294, 295, 463, 571
BstSFI CTRYAG 3 cut(s) 312, 547, 582
BstV1I GCAGC 4 cut(s) 22, 307, 590, 709
BstV2I GAAGAC 1 cut(s) 377
BstX2I RGATCY 3 cut(s) 593, 685, 797
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 3 cut(s) 593, 685, 797
Bsu15I ATCGAT 1 cut(s) 153
BsuRI GGCC 1 cut(s) 665
BsuTUI ATCGAT 1 cut(s) 153
BtgZI GCGATG 1 cut(s) 113
BtsCI GGATG 5 cut(s) 183, 364, 460, 568, 740
BtsIMutI CAGTG 1 cut(s) 224
Cac8I GCNNGC 3 cut(s) 164, 304, 423
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 656
Cfr9I CCCGGG 1 cut(s) 295
ClaI ATCGAT 1 cut(s) 153
Csp6I GTAC 1 cut(s) 367
CviAII CATG 2 cut(s) 251, 290
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 26, 605
DpnI GATC 7 cut(s) 174, 354, 417, 595, 687, 741, 799
DpnII GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 3 cut(s) 603, 649, 771
EarI CTCTTC 3 cut(s) 603, 649, 771
Ecl136II GAGCTC 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 656
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 38
Eco88I CYCGRG 1 cut(s) 295
EcoICRI GAGCTC 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 656
EcoRII CCWGG 2 cut(s) 254, 463
EcoT38I GRGCYC 1 cut(s) 168
FaeI CATG 2 cut(s) 254, 293
FaiI YATR 9 cut(s) 252, 291, 314, 347, 435, 437, 549, 584, 692
FaqI GGGAC 2 cut(s) 278, 579
FatI CATG 2 cut(s) 250, 289
FblI GTMKAC 2 cut(s) 141, 450
Fnu4HI GCNGC 7 cut(s) 11, 14, 178, 321, 339, 579, 723
FokI GGATG 5 cut(s) 170, 371, 447, 555, 747
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 7 cut(s) 11, 14, 178, 321, 339, 579, 723
GlaI GCGC 1 cut(s) 127
GluI GCNGC 7 cut(s) 11, 14, 178, 321, 339, 579, 723
GsuI CTGGAG 2 cut(s) 142, 248
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 1 cut(s) 665
HapII CCGG 4 cut(s) 296, 356, 573, 795
HhaI GCGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 254, 293
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 2 cut(s) 142, 451
HindII GTYRAC 2 cut(s) 142, 451
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 4 cut(s) 48, 147, 626, 711
HpaII CCGG 4 cut(s) 296, 356, 573, 795
HphI GGTGA 2 cut(s) 43, 362
Hpy166II GTNNAC 2 cut(s) 142, 451
Hpy188I TCNGA 5 cut(s) 35, 415, 498, 559, 802
Hpy188III TCNNGA 2 cut(s) 356, 715
Hpy8I GTNNAC 2 cut(s) 142, 451
HpyAV CCTTC 4 cut(s) 88, 199, 436, 488
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 8 cut(s) 4, 79, 277, 306, 362, 460, 533, 578
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 22, 230, 308, 317, 584, 728
HpyF3I CTNAG 2 cut(s) 26, 605
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 2 cut(s) 254, 293
HspAI GCGC 1 cut(s) 126
Kpn2I TCCGGA 1 cut(s) 355
Kzo9I GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
LmnI GCTCC 4 cut(s) 123, 200, 299, 317
Lsp1109I GCAGC 4 cut(s) 22, 307, 590, 709
LweI GCATC 4 cut(s) 66, 349, 469, 563
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 631, 679
MalI GATC 7 cut(s) 174, 354, 417, 595, 687, 741, 799
MboI GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
MboII GAAGA 6 cut(s) 31, 377, 590, 593, 636, 788
MflI RGATCY 3 cut(s) 593, 685, 797
MhlI GDGCHC 2 cut(s) 168, 472
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 4 cut(s) 56, 211, 238, 272
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 2 cut(s) 42, 156
MmeI TCCRAC 2 cut(s) 58, 738
Mox20I TGGCCA 1 cut(s) 665
MroI TCCGGA 1 cut(s) 355
MscI TGGCCA 1 cut(s) 665
MseI TTAA 1 cut(s) 750
MslI CAYNNNNRTG 3 cut(s) 465, 573, 695
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 2 cut(s) 13, 67
MspI CCGG 4 cut(s) 296, 356, 573, 795
MspR9I CCNGG 5 cut(s) 256, 296, 297, 465, 573
MvaI CCWGG 2 cut(s) 256, 465
MwoI GCNNNNNNNGC 7 cut(s) 10, 22, 230, 308, 317, 584, 728
NciI CCSGG 3 cut(s) 296, 297, 573
NdeII GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
NlaIII CATG 2 cut(s) 254, 293
NlaIV GGNNCC 2 cut(s) 301, 657
NmuCI GTSAC 1 cut(s) 631
NspV TTCGAA 1 cut(s) 242
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 2 cut(s) 626, 711
PflFI GACNNNGTC 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 261
PkrI GCNGC 7 cut(s) 12, 15, 179, 322, 340, 580, 724
Ple19I CGATCG 1 cut(s) 175
PleI GAGTC 2 cut(s) 42, 155
PpsI GAGTC 2 cut(s) 42, 155
PpuMI RGGWCCY 1 cut(s) 656
Psp124BI GAGCTC 1 cut(s) 168
Psp5II RGGWCCY 1 cut(s) 656
Psp6I CCWGG 2 cut(s) 254, 463
PspGI CCWGG 2 cut(s) 254, 463
PspN4I GGNNCC 2 cut(s) 301, 657
PspPI GGNCC 1 cut(s) 656
PspPPI RGGWCCY 1 cut(s) 656
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 3 cut(s) 593, 685, 797
PsyI GACNNNGTC 1 cut(s) 146
PvuI CGATCG 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 13
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
RseI CAYNNNNRTG 3 cut(s) 465, 573, 695
SacI GAGCTC 1 cut(s) 168
SalI GTCGAC 2 cut(s) 140, 449
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 7 cut(s) 11, 14, 178, 321, 339, 579, 723
Sau3AI GATC 7 cut(s) 172, 352, 415, 593, 685, 739, 797
Sau96I GGNCC 1 cut(s) 656
SchI GAGTC 2 cut(s) 42, 156
ScrFI CCNGG 5 cut(s) 256, 296, 297, 465, 573
SduI GDGCHC 2 cut(s) 168, 472
SfaNI GCATC 4 cut(s) 66, 349, 469, 563
SfcI CTRYAG 3 cut(s) 312, 547, 582
SfuI TTCGAA 1 cut(s) 242
SinI GGWCC 1 cut(s) 656
SmaI CCCGGG 1 cut(s) 297
SmiMI CAYNNNNRTG 3 cut(s) 465, 573, 695
SmlI CTYRAG 2 cut(s) 131, 767
SmoI CTYRAG 2 cut(s) 131, 767
Sse9I AATT 4 cut(s) 56, 211, 238, 272
SsiI CCGC 3 cut(s) 67, 178, 338
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 5 cut(s) 254, 294, 295, 463, 571
TaaI ACNGT 1 cut(s) 366
TaiI ACGT 1 cut(s) 408
TaqI TCGA 5 cut(s) 141, 153, 242, 388, 450
TaqII GACCGA 1 cut(s) 159
TasI AATT 4 cut(s) 56, 211, 238, 272
TauI GCSGC 2 cut(s) 180, 341
TfiI GAWTC 2 cut(s) 626, 711
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TscAI CASTG 1 cut(s) 231
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 5 cut(s) 10, 13, 320, 578, 722
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 1 cut(s) 414
TspGWI ACGGA 1 cut(s) 619
TspMI CCCGGG 1 cut(s) 295
TspRI CASTG 1 cut(s) 231
Tth111I GACNNNGTC 1 cut(s) 146
Van91I CCANNNNNTGG 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 656
XapI RAATTY 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 295
XmiI GTMKAC 2 cut(s) 141, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.