MD02G1080400.v1.1

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011769
Physical Location & Seq
Forward (+)
6326967 .. 6328088
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1080400.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGACGGCATCTCTCACCATTTTATATATCCGTTTGTGTCATCCTACTCACCAAAACCCCACGGTACTCCATTTTCATCTGCCATACAACAACAAAAATAACTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTAGAAGCTCACAGGAGTGAGAAGCAGCTGAGAAACTGCAACATGGCGGACCCGTCGTTGTCTTTGGCCATGTGGGCATGGATGCTCAGCTGGTTCACGCCCTCCTCGCTCTTCATCCTCCTCAACCTCATGATCGGCATCATAGTCCTCAGCTCCCGCTATGAGACCCACAAAAAACCTGAGCACCAACAACAACTGCAGCAGCAAGAAGAGCAACCCGGCCTGTTCAGCTCGCCTCAACTCGAACGAACCCCCTCACTCTTGGACCGGGTCCGGTCGATAAACTTCTCCCACTACAAATTCGAGCAACCGAATCCTGAAACCCATTACACGCCGCCACAACATGCAGATTTAGATAATTCGACCGGATCAAACCGGACTCCCTCACCCCTACTCGAACGAACTCCCTCGCTATTGGACCGGTTTCGGTCCATCAACTTTTCCCACTACCAATTTGAACAATCCACCAGCCCAGAAGTCCAATCCGGCGAATCAGCAGCACAACATCCTGGTTCGAGTCAGACAGACCGCAATAGTCCGGTCGGGTTAGCTAGGACACCCTCGCTCTTGGAGCGGCTCAAGTCGATGGATTTCCCATTCTACCGGCCCGCACATGCGGACCCAGAGAATAAAATTCATGAACCGGAAGCGTCCGAACATGAGGCCCACGATCCAAGTTCGAGTGAAGAAAACTTGGTTCATCGGAATAAGTCGAACTCGGGTAATGGCGCTCGGGTAAACCAGCATGAGAAGATAAAAAAGTCGGCCAGCGAGAACTCGCGGCTCCGAGGAGGGGTTGAAGGAAACAACAACGAAACGGACATTAGGCCAGCGACGCCGAGGGTGAAGAAAACGAGATCGTTTGGAGGACACGAAGCAGTTGACGCGAAAGCTAACGATTTCATAAACAAGTTTAAGCAGCAGCTGAGACAGCAAAGAGTTCAAGCAGCAGCTGAGACTGCAGAGGCTCGAATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

374

Amino Acids

42.22

Weight (kDa)

7.91

Isoelectric Point (pI)

54.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 71 - 101 7.1e-11 Domain of unknown function (DUF4408)
DUF761 PF05553 339 - 362 3.6e-07 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26130 AT5G56980
fragaria_vesca FvH4_1g07560
malus_domestica MD02G1080400.v1.1 MD15G1207800.v1.1
prunus_persica Prupe.7G209100_v2.0.a1
pyrus_communis pycom15g18410
rosa_laevigata RLG00000016403
rosa_multiflora Rmu_co8329807.1_g000001 Rmu_sc0004086.1_g000010 Rmu_sc0021911.1_g000006
rosa_roxburghii Rroxscaffold_2G00148090
rosa_rugosa Rorug02G0036200 Rorug02G0036300
rosa_samantha Rh2AG082500 Rh2BG083300 Rh2CG085300 Rh2DG081400
rosa_wichuraiana Rw2G006470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 715
AccII CGCG 2 cut(s) 922, 1028
AciI CCGC 8 cut(s) 188, 298, 476, 670, 715, 750, 758, 922
AclWI GGATC 2 cut(s) 517, 806
AcoI YGGCCR 2 cut(s) 207, 906
AcsI RAATTY 2 cut(s) 440, 774
AcyI GRCGYC 1 cut(s) 977
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 934
AgeI ACCGGT 1 cut(s) 561
AgsI TTSAA 3 cut(s) 599, 941, 1085
AjnI CCWGG 1 cut(s) 649
AleI CACNNNNGTG 1 cut(s) 156
AluBI AGCT 9 cut(s) 149, 169, 231, 294, 372, 692, 1034, 1066, 1094
AluI AGCT 9 cut(s) 149, 169, 231, 294, 372, 692, 1034, 1066, 1094
Alw21I GWGCWC 1 cut(s) 327
Alw26I GTCTC 3 cut(s) 299, 1063, 1091
AlwI GGATC 2 cut(s) 517, 806
AlwNI CAGNNNCTG 2 cut(s) 1066, 1094
Ama87I CYCGRG 2 cut(s) 859, 873
AoxI GGCC 6 cut(s) 207, 361, 746, 804, 906, 968
ApeKI GCWGC 8 cut(s) 166, 340, 343, 638, 1060, 1063, 1088, 1091
ApoI RAATTY 2 cut(s) 440, 774
AsiGI ACCGGT 1 cut(s) 561
AspLEI GCGC 1 cut(s) 872
AspS9I GGNCC 8 cut(s) 190, 406, 412, 559, 570, 747, 760, 805
AsuC2I CCSGG 2 cut(s) 360, 410
AsuHPI GGTGA 4 cut(s) 7, 41, 519, 997
AvaI CYCGRG 2 cut(s) 859, 873
AvaII GGWCC 6 cut(s) 190, 406, 412, 559, 570, 760
BalI TGGCCA 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 290
BbvI GCAGC 8 cut(s) 178, 352, 355, 650, 1072, 1075, 1100, 1103
BccI CCATC 2 cut(s) 581, 721
BceAI ACGGC 1 cut(s) 21
BciT130I CCWGG 1 cut(s) 651
BcnI CCSGG 2 cut(s) 360, 410
BcoDI GTCTC 3 cut(s) 299, 1063, 1091
BfaI CTAG 2 cut(s) 143, 693
BfmI CTRYAG 2 cut(s) 338, 1101
BfoI RGCGCY 1 cut(s) 873
BglI GCCNNNNNGGC 1 cut(s) 215
BlpI GCTNAGC 1 cut(s) 227
Bme1390I CCNGG 3 cut(s) 360, 410, 651
Bme18I GGWCC 6 cut(s) 190, 406, 412, 559, 570, 760
BmeT110I CYCGRG 2 cut(s) 859, 873
BmgT120I GGNCC 8 cut(s) 190, 406, 412, 559, 570, 747, 760, 805
BmiI GGNNCC 4 cut(s) 192, 413, 762, 926
BmrFI CCNGG 3 cut(s) 360, 410, 651
BmsI GCATC 3 cut(s) 17, 213, 288
Bpu10I CCTNAGC 2 cut(s) 290, 321
Bpu1102I GCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 704
BpuMI CCSGG 2 cut(s) 360, 410
BsaBI GATNNNNATC 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 977
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 3 cut(s) 60, 928, 980
BsaWI WCCGGW 6 cut(s) 414, 506, 516, 561, 679, 784
Bsc4I CCNNNNNNNGG 1 cut(s) 934
Bse118I RCCGGY 2 cut(s) 561, 744
Bse8I GATNNNNATC 1 cut(s) 278
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 3 cut(s) 60, 928, 980
BseGI GGATG 4 cut(s) 40, 228, 255, 646
BseJI GATNNNNATC 1 cut(s) 278
BseLI CCNNNNNNNGG 1 cut(s) 934
BseMII CTCAG 6 cut(s) 161, 241, 304, 312, 1058, 1086
BseRI GAGGAG 3 cut(s) 235, 251, 945
BseXI GCAGC 8 cut(s) 178, 352, 355, 650, 1072, 1075, 1100, 1103
Bsh1236I CGCG 2 cut(s) 922, 1028
Bsh1285I CGRYCG 3 cut(s) 419, 507, 684
BshFI GGCC 6 cut(s) 209, 363, 748, 806, 908, 970
BshTI ACCGGT 1 cut(s) 561
BsiEI CGRYCG 3 cut(s) 419, 507, 684
BsiHKAI GWGCWC 1 cut(s) 327
BsiHKCI CYCGRG 2 cut(s) 859, 873
BslI CCNNNNNNNGG 1 cut(s) 934
BsmAI GTCTC 3 cut(s) 299, 1063, 1091
BsnI GGCC 6 cut(s) 209, 363, 748, 806, 908, 970
Bso31I GGTCTC 1 cut(s) 299
BsoBI CYCGRG 2 cut(s) 859, 873
Bsp1286I GDGCHC 1 cut(s) 327
Bsp143I GATC 4 cut(s) 273, 509, 811, 998
Bsp1720I GCTNAGC 1 cut(s) 227
BspACI CCGC 8 cut(s) 188, 298, 476, 670, 715, 750, 758, 922
BspANI GGCC 6 cut(s) 209, 363, 748, 806, 908, 970
BspCNI CTCAG 6 cut(s) 162, 240, 303, 313, 1059, 1087
BspFNI CGCG 2 cut(s) 922, 1028
BspHI TCATGA 2 cut(s) 270, 778
BspLI GGNNCC 4 cut(s) 192, 413, 762, 926
BspMAI CTGCAG 2 cut(s) 342, 1105
BspPI GGATC 2 cut(s) 517, 806
BspQI GCTCTTC 2 cut(s) 257, 345
BspTNI GGTCTC 1 cut(s) 299
BsrBI CCGCTC 1 cut(s) 715
BsrFI RCCGGY 2 cut(s) 561, 744
BssAI RCCGGY 2 cut(s) 561, 744
BssECI CCNNGG 3 cut(s) 60, 928, 980
BssMI GATC 4 cut(s) 273, 509, 811, 998
BssNI GRCGYC 1 cut(s) 977
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 2 cut(s) 257, 345
BstACI GRCGYC 1 cut(s) 977
BstC8I GCNNGC 4 cut(s) 374, 750, 910, 972
BstDEI CTNAG 6 cut(s) 170, 227, 290, 321, 1067, 1095
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 4 cut(s) 40, 228, 255, 646
BstFNI CGCG 2 cut(s) 922, 1028
BstH2I RGCGCY 1 cut(s) 873
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 4 cut(s) 276, 512, 814, 1001
BstMAI GTCTC 3 cut(s) 299, 1063, 1091
BstMBI GATC 4 cut(s) 273, 509, 811, 998
BstMCI CGRYCG 3 cut(s) 419, 507, 684
BstMWI GCNNNNNNNGC 9 cut(s) 215, 247, 352, 369, 712, 976, 1025, 1072, 1100
BstNI CCWGG 1 cut(s) 651
BstNSI RCATGY 2 cut(s) 488, 758
BstSCI CCNGG 3 cut(s) 358, 408, 649
BstSFI CTRYAG 2 cut(s) 338, 1101
BstUI CGCG 2 cut(s) 922, 1028
BstV1I GCAGC 8 cut(s) 178, 352, 355, 650, 1072, 1075, 1100, 1103
BsuRI GGCC 6 cut(s) 209, 363, 748, 806, 908, 970
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 4 cut(s) 40, 228, 255, 646
Cac8I GCNNGC 4 cut(s) 374, 750, 910, 972
CaiI CAGNNNCTG 2 cut(s) 1066, 1094
CciI TCATGA 2 cut(s) 270, 778
CfoI GCGC 1 cut(s) 872
Cfr10I RCCGGY 2 cut(s) 561, 744
Cfr13I GGNCC 8 cut(s) 190, 406, 412, 559, 570, 747, 760, 805
CseI GACGC 3 cut(s) 780, 985, 1034
Csp6I GTAC 1 cut(s) 65
CspAI ACCGGT 1 cut(s) 561
CviAII CATG 9 cut(s) 184, 211, 219, 271, 485, 755, 779, 800, 887
CviQI GTAC 1 cut(s) 65
DdeI CTNAG 6 cut(s) 170, 227, 290, 321, 1067, 1095
DpnI GATC 4 cut(s) 275, 511, 813, 1000
DpnII GATC 4 cut(s) 273, 509, 811, 998
EaeI YGGCCR 2 cut(s) 207, 906
Eam1104I CTCTTC 2 cut(s) 257, 345
EarI CTCTTC 2 cut(s) 257, 345
EciI GGCGGA 1 cut(s) 203
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 6 cut(s) 190, 406, 412, 559, 570, 760
Eco88I CYCGRG 2 cut(s) 859, 873
EcoRII CCWGG 1 cut(s) 649
FaeI CATG 9 cut(s) 187, 214, 222, 274, 488, 758, 782, 803, 890
FatI CATG 9 cut(s) 183, 210, 218, 270, 484, 754, 778, 799, 886
FauI CCCGC 2 cut(s) 305, 757
FokI GGATG 4 cut(s) 27, 235, 242, 633
FspBI CTAG 2 cut(s) 143, 693
GlaI GCGC 1 cut(s) 871
HaeII RGCGCY 1 cut(s) 873
HaeIII GGCC 6 cut(s) 209, 363, 748, 806, 908, 970
HgaI GACGC 3 cut(s) 780, 985, 1034
HhaI GCGC 1 cut(s) 872
Hin1I GRCGYC 1 cut(s) 977
Hin1II CATG 9 cut(s) 187, 214, 222, 274, 488, 758, 782, 803, 890
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HinfI GANTC 5 cut(s) 454, 520, 632, 658, 1113
HphI GGTGA 4 cut(s) 7, 41, 519, 997
Hpy166II GTNNAC 3 cut(s) 237, 880, 1024
Hpy188I TCNGA 4 cut(s) 663, 796, 846, 929
Hpy188III TCNNGA 4 cut(s) 143, 271, 458, 779
Hpy8I GTNNAC 3 cut(s) 237, 880, 1024
Hpy99I CGWCG 2 cut(s) 199, 979
HpyAV CCTTC 1 cut(s) 935
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4V TGCA 4 cut(s) 180, 340, 488, 1103
HpyF10VI GCNNNNNNNGC 9 cut(s) 215, 247, 352, 369, 712, 976, 1025, 1072, 1100
HpyF3I CTNAG 6 cut(s) 170, 227, 290, 321, 1067, 1095
Hsp92I GRCGYC 1 cut(s) 977
Hsp92II CATG 9 cut(s) 187, 214, 222, 274, 488, 758, 782, 803, 890
HspAI GCGC 1 cut(s) 870
Kzo9I GATC 4 cut(s) 273, 509, 811, 998
LguI GCTCTTC 2 cut(s) 257, 345
LmnI GCTCC 3 cut(s) 299, 712, 930
Lsp1109I GCAGC 8 cut(s) 178, 352, 355, 650, 1072, 1075, 1100, 1103
LweI GCATC 3 cut(s) 17, 213, 288
MaeI CTAG 2 cut(s) 143, 693
MalI GATC 4 cut(s) 275, 511, 813, 1000
MbiI CCGCTC 1 cut(s) 715
MboI GATC 4 cut(s) 273, 509, 811, 998
MboII GAAGA 5 cut(s) 244, 362, 839, 904, 1000
MhlI GDGCHC 1 cut(s) 327
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 5 cut(s) 440, 499, 593, 774, 1117
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 2 cut(s) 514, 667
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
MseI TTAA 2 cut(s) 1056, 1120
MslI CAYNNNNRTG 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 209
MspA1I CMGCKG 4 cut(s) 169, 231, 1066, 1094
MspR9I CCNGG 3 cut(s) 360, 410, 651
MvaI CCWGG 1 cut(s) 651
MvnI CGCG 2 cut(s) 922, 1028
MwoI GCNNNNNNNGC 9 cut(s) 215, 247, 352, 369, 712, 976, 1025, 1072, 1100
NciI CCSGG 2 cut(s) 360, 410
NdeII GATC 4 cut(s) 273, 509, 811, 998
NlaIII CATG 9 cut(s) 187, 214, 222, 274, 488, 758, 782, 803, 890
NlaIV GGNNCC 4 cut(s) 192, 413, 762, 926
NmeAIII GCCGAG 1 cut(s) 1005
NspI RCATGY 2 cut(s) 488, 758
OliI CACNNNNGTG 1 cut(s) 156
PagI TCATGA 2 cut(s) 270, 778
PciSI GCTCTTC 2 cut(s) 257, 345
PfeI GAWTC 3 cut(s) 454, 632, 1113
PflFI GACNNNGTC 1 cut(s) 410
PinAI ACCGGT 1 cut(s) 561
PleI GAGTC 2 cut(s) 514, 666
PpsI GAGTC 2 cut(s) 514, 666
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 4 cut(s) 192, 413, 762, 926
PspPI GGNCC 8 cut(s) 190, 406, 412, 559, 570, 747, 760, 805
PstI CTGCAG 2 cut(s) 342, 1105
PstNI CAGNNNCTG 2 cut(s) 1066, 1094
PsyI GACNNNGTC 1 cut(s) 410
PvuII CAGCTG 4 cut(s) 169, 231, 1066, 1094
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
RseI CAYNNNNRTG 1 cut(s) 156
SapI GCTCTTC 2 cut(s) 257, 345
SaqAI TTAA 2 cut(s) 1056, 1120
Sau3AI GATC 4 cut(s) 273, 509, 811, 998
Sau96I GGNCC 8 cut(s) 190, 406, 412, 559, 570, 747, 760, 805
SchI GAGTC 2 cut(s) 514, 667
ScrFI CCNGG 3 cut(s) 360, 410, 651
SduI GDGCHC 1 cut(s) 327
SfaNI GCATC 3 cut(s) 17, 213, 288
SfcI CTRYAG 2 cut(s) 338, 1101
SinI GGWCC 6 cut(s) 190, 406, 412, 559, 570, 760
SmiMI CAYNNNNRTG 1 cut(s) 156
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 5 cut(s) 440, 499, 593, 774, 1117
SsiI CCGC 8 cut(s) 188, 298, 476, 670, 715, 750, 758, 922
SspMI CTAG 2 cut(s) 143, 693
StyD4I CCNGG 3 cut(s) 358, 408, 649
TaaI ACNGT 1 cut(s) 64
TaqII GACCGA 1 cut(s) 558
TasI AATT 5 cut(s) 440, 499, 593, 774, 1117
TauI GCSGC 3 cut(s) 478, 718, 925
TfiI GAWTC 3 cut(s) 454, 632, 1113
Tru1I TTAA 2 cut(s) 1056, 1120
Tru9I TTAA 2 cut(s) 1056, 1120
TseI GCWGC 8 cut(s) 166, 340, 343, 638, 1060, 1063, 1088, 1091
TspDTI ATGAA 6 cut(s) 65, 244, 767, 795, 830, 1033
TspGWI ACGGA 2 cut(s) 20, 974
Tth111I GACNNNGTC 1 cut(s) 410
VpaK11BI GGWCC 6 cut(s) 190, 406, 412, 559, 570, 760
XapI RAATTY 2 cut(s) 440, 774
XbaI TCTAGA 1 cut(s) 142
XceI RCATGY 2 cut(s) 488, 758
XspI CTAG 2 cut(s) 143, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.