Rw2G006470

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011769
Physical Location & Seq
Reverse (-)
6215846 .. 6217021
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006470.1

Sequence Viewer

Length: 924 bp
ATGGCAGCTATGTTGGCTTTCATGCTCAGCTGGCTCACACCCACCTCCCTCTTCATCTTCCTCAACCTCGCCATCGGCACCCTCGTCATCTCCCACCGCTTCTGCAAAACACCTCAGCAACAACCCCAACACGACCAGTTCGGTAGACCGCCCTCGCTCCTGCAACGTGTCAGGTCGTTCGACTTCTCCCACTACAACTTCCAACCCGATCACCCGGAACCCCATCACGTAACGGAAAACCCGACCGGATTAGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCACTACAACTTCCAACATCACTACCCGGAACCCGAACACGTAACGGAATACCCAGCACCGGACAACCCGACCGGATTTGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCAGTACGACTTCCAACAACCCGACCCGGAACCCGAACACGTAACAGAACACCCAACACCGGAAAACCCGACCGGAATAGTCCGGACGCCCTCGCTCCTGGCCCGTCTCAAGTCAATGGATTTCTCCTCTCTCTACAGATCCGACCCGGAAGCTGAAGATCTTCATCCGACCGATGCCCATAACCCAAACCCGAACCCGAAGCCGAAGCCGACGCGAGACCATATGGTTCATCGGAGCAAGTCGGACAGGGGTCATGAAGGTTCGGAAATCCGACCCGAAAAGGTGATAAAGAAGTCCGCCAGCGAGAGAGGATTCGAAGAAAACGAGGGAGGTGTCCGGCAGCGAAGGGGGGTGGAGAAAACGATGTCGTTTGGAGATGATGAAGAAGTTGACGCGAAAGCTGACGACTTTATTAACCGGTTCAAGCAGCAGCTCAAGTTACAGAGGCTCGACTCGCTTTTGCGTTACAGCAGAAATGCTCCAGAGAAACTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

307

Amino Acids

35.53

Weight (kDa)

6.5

Isoelectric Point (pI)

48.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 4 - 34 1.3e-09 Domain of unknown function (DUF4408)
DUF761 PF05553 269 - 299 2.8e-14 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26130 AT5G56980
fragaria_vesca FvH4_1g07560
malus_domestica MD02G1080400.v1.1 MD15G1207800.v1.1
prunus_persica Prupe.7G209100_v2.0.a1
pyrus_communis pycom15g18410
rosa_laevigata RLG00000016403
rosa_multiflora Rmu_co8329807.1_g000001 Rmu_sc0004086.1_g000010 Rmu_sc0021911.1_g000006
rosa_roxburghii Rroxscaffold_2G00148090
rosa_rugosa Rorug02G0036200 Rorug02G0036300
rosa_samantha Rh2AG082500 Rh2BG083300 Rh2CG085300 Rh2DG081400
rosa_wichuraiana Rw2G006470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 2 cut(s) 277, 403
AccI GTMKAC 1 cut(s) 145
AccII CGCG 2 cut(s) 640, 821
AccIII TCCGGA 3 cut(s) 255, 381, 507
AciI CCGC 5 cut(s) 97, 149, 277, 403, 723
AclWI GGATC 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 358, 484
AflIII ACRYGT 3 cut(s) 166, 337, 463
AgeI ACCGGT 1 cut(s) 843
AgsI TTSAA 1 cut(s) 850
AjnI CCWGG 1 cut(s) 522
AluBI AGCT 5 cut(s) 8, 30, 578, 827, 859
AluI AGCT 5 cut(s) 8, 30, 578, 827, 859
Alw26I GTCTC 2 cut(s) 536, 636
AlwI GGATC 1 cut(s) 558
Aor13HI TCCGGA 3 cut(s) 255, 381, 507
AoxI GGCC 1 cut(s) 525
ApeKI GCWGC 4 cut(s) 5, 766, 853, 856
AsiGI ACCGGT 1 cut(s) 843
Asp700I GAANNNNTTC 1 cut(s) 585
AspS9I GGNCC 3 cut(s) 285, 411, 526
AsuC2I CCSGG 4 cut(s) 215, 326, 452, 572
AsuHPI GGTGA 2 cut(s) 203, 721
AsuII TTCGAA 1 cut(s) 741
AvaII GGWCC 2 cut(s) 285, 411
BanI GGYRCC 1 cut(s) 77
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 4 cut(s) 17, 778, 865, 868
BccI CCATC 4 cut(s) 80, 231, 296, 422
BciT130I CCWGG 1 cut(s) 524
BcnI CCSGG 4 cut(s) 215, 326, 452, 572
BcoDI GTCTC 2 cut(s) 536, 636
BfmI CTRYAG 1 cut(s) 559
BglII AGATCT 1 cut(s) 583
BisI GCNGC 6 cut(s) 6, 278, 404, 767, 854, 857
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 6 cut(s) 7, 279, 405, 768, 855, 858
Bme1390I CCNGG 5 cut(s) 215, 326, 452, 524, 572
Bme18I GGWCC 2 cut(s) 285, 411
BmgT120I GGNCC 3 cut(s) 285, 411, 526
BmiI GGNNCC 4 cut(s) 79, 219, 330, 456
BmrFI CCNGG 5 cut(s) 215, 326, 452, 524, 572
BmrI ACTGGG 1 cut(s) 421
BmsI GCATC 1 cut(s) 589
BmuI ACTGGG 1 cut(s) 421
BoxI GACNNNNGTC 1 cut(s) 675
BpmI CTGGAG 1 cut(s) 891
Bpu10I CCTNAGC 3 cut(s) 114, 281, 407
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 518, 845
BpuMI CCSGG 4 cut(s) 215, 326, 452, 572
BsaAI YACGTR 3 cut(s) 229, 340, 466
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 636
BsaWI WCCGGW 9 cut(s) 245, 255, 358, 371, 381, 484, 497, 507, 843
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 484
Bse118I RCCGGY 1 cut(s) 843
Bse1I ACTGG 2 cut(s) 136, 427
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 3 cut(s) 255, 381, 507
BseBI CCWGG 1 cut(s) 524
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 2 cut(s) 358, 484
BseMII CTCAG 4 cut(s) 40, 128, 295, 421
BseNI ACTGG 2 cut(s) 136, 427
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 4 cut(s) 17, 778, 865, 868
BseYI CCCAGC 1 cut(s) 352
Bsh1236I CGCG 2 cut(s) 640, 821
Bsh1285I CGRYCG 4 cut(s) 246, 372, 498, 597
BshFI GGCC 1 cut(s) 527
BshNI GGYRCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 843
BsiEI CGRYCG 4 cut(s) 246, 372, 498, 597
BslI CCNNNNNNNGG 2 cut(s) 358, 484
BsmAI GTCTC 2 cut(s) 536, 636
BsmBI CGTCTC 1 cut(s) 536
BsnI GGCC 1 cut(s) 527
Bso31I GGTCTC 1 cut(s) 636
Bsp119I TTCGAA 1 cut(s) 741
Bsp13I TCCGGA 3 cut(s) 255, 381, 507
Bsp143I GATC 3 cut(s) 208, 563, 583
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 5 cut(s) 97, 149, 277, 403, 723
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 4 cut(s) 39, 127, 294, 420
BspEI TCCGGA 3 cut(s) 255, 381, 507
BspFNI CGCG 2 cut(s) 640, 821
BspHI TCATGA 1 cut(s) 679
BspLI GGNNCC 4 cut(s) 79, 219, 330, 456
BspPI GGATC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 741
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 636
BsrBI CCGCTC 2 cut(s) 277, 403
BsrFI RCCGGY 1 cut(s) 843
BsrI ACTGG 2 cut(s) 136, 427
BssAI RCCGGY 1 cut(s) 843
BssMI GATC 3 cut(s) 208, 563, 583
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 1 cut(s) 524
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 512
BstBAI YACGTR 3 cut(s) 229, 340, 466
BstBI TTCGAA 1 cut(s) 741
BstC8I GCNNGC 2 cut(s) 32, 727
BstDEI CTNAG 4 cut(s) 26, 114, 281, 407
BstF5I GGATG 1 cut(s) 589
BstFNI CGCG 2 cut(s) 640, 821
BstKTI GATC 3 cut(s) 211, 566, 586
BstMAI GTCTC 2 cut(s) 536, 636
BstMBI GATC 3 cut(s) 208, 563, 583
BstMCI CGRYCG 4 cut(s) 246, 372, 498, 597
BstMWI GCNNNNNNNGC 3 cut(s) 14, 31, 880
BstNI CCWGG 1 cut(s) 524
BstPAI GACNNNNGTC 1 cut(s) 675
BstSCI CCNGG 5 cut(s) 213, 324, 450, 522, 570
BstSFI CTRYAG 1 cut(s) 559
BstUI CGCG 2 cut(s) 640, 821
BstV1I GCAGC 4 cut(s) 17, 778, 865, 868
BstX2I RGATCY 2 cut(s) 563, 583
BstYI RGATCY 2 cut(s) 563, 583
BsuRI GGCC 1 cut(s) 527
BtsCI GGATG 1 cut(s) 589
Cac8I GCNNGC 2 cut(s) 32, 727
CciI TCATGA 1 cut(s) 679
Cfr10I RCCGGY 1 cut(s) 843
Cfr13I GGNCC 3 cut(s) 285, 411, 526
CseI GACGC 3 cut(s) 520, 646, 827
Csp6I GTAC 1 cut(s) 430
CspAI ACCGGT 1 cut(s) 843
CviAII CATG 4 cut(s) 22, 271, 397, 680
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 4 cut(s) 26, 114, 281, 407
DpnI GATC 3 cut(s) 210, 565, 585
DpnII GATC 3 cut(s) 208, 563, 583
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 712
Eco31I GGTCTC 1 cut(s) 636
Eco47I GGWCC 2 cut(s) 285, 411
Eco57I CTGAAG 1 cut(s) 600
EcoRII CCWGG 1 cut(s) 522
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 4 cut(s) 25, 274, 400, 683
FaiI YATR 8 cut(s) 11, 23, 272, 398, 606, 648, 650, 681
FatI CATG 4 cut(s) 21, 270, 396, 679
FauNDI CATATG 1 cut(s) 648
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 6 cut(s) 6, 278, 404, 767, 854, 857
FokI GGATG 1 cut(s) 576
Fsp4HI GCNGC 6 cut(s) 6, 278, 404, 767, 854, 857
GluI GCNGC 6 cut(s) 6, 278, 404, 767, 854, 857
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 1 cut(s) 891
HaeIII GGCC 1 cut(s) 527
HgaI GACGC 3 cut(s) 520, 646, 827
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 4 cut(s) 25, 274, 400, 683
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 4 cut(s) 259, 385, 738, 878
HphI GGTGA 2 cut(s) 203, 721
Hpy166II GTNNAC 2 cut(s) 146, 817
Hpy188I TCNGA 6 cut(s) 568, 594, 660, 670, 691, 698
Hpy188III TCNNGA 5 cut(s) 256, 382, 508, 680, 908
Hpy8I GTNNAC 2 cut(s) 146, 817
Hpy99I CGWCG 1 cut(s) 640
HpyAV CCTTC 2 cut(s) 677, 765
HpyCH4IV ACGT 4 cut(s) 166, 228, 339, 465
HpyCH4V TGCA 2 cut(s) 105, 163
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 31, 880
HpyF3I CTNAG 4 cut(s) 26, 114, 281, 407
HpySE526I ACGT 4 cut(s) 166, 228, 339, 465
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 4 cut(s) 25, 274, 400, 683
Kpn2I TCCGGA 3 cut(s) 255, 381, 507
Kzo9I GATC 3 cut(s) 208, 563, 583
LmnI GCTCC 6 cut(s) 162, 274, 400, 525, 660, 910
Lsp1109I GCAGC 4 cut(s) 17, 778, 865, 868
LweI GCATC 1 cut(s) 589
MaeII ACGT 4 cut(s) 166, 228, 339, 465
MaeIII GTNAC 7 cut(s) 229, 264, 340, 390, 466, 864, 890
MalI GATC 3 cut(s) 210, 565, 585
MbiI CCGCTC 2 cut(s) 277, 403
MboI GATC 3 cut(s) 208, 563, 583
MboII GAAGA 6 cut(s) 43, 49, 578, 593, 755, 821
MflI RGATCY 2 cut(s) 563, 583
MluCI AATT 1 cut(s) 919
MlyI GAGTC 3 cut(s) 253, 379, 872
MmeI TCCRAC 7 cut(s) 226, 337, 463, 591, 617, 648, 721
MroI TCCGGA 3 cut(s) 255, 381, 507
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 2 cut(s) 840, 922
MspA1I CMGCKG 1 cut(s) 30
MspR9I CCNGG 5 cut(s) 215, 326, 452, 524, 572
MvaI CCWGG 1 cut(s) 524
MvnI CGCG 2 cut(s) 640, 821
MwoI GCNNNNNNNGC 3 cut(s) 14, 31, 880
NciI CCSGG 4 cut(s) 215, 326, 452, 572
NdeI CATATG 1 cut(s) 648
NdeII GATC 3 cut(s) 208, 563, 583
NlaIII CATG 4 cut(s) 25, 274, 400, 683
NlaIV GGNNCC 4 cut(s) 79, 219, 330, 456
NmuCI GTSAC 2 cut(s) 264, 390
NspV TTCGAA 1 cut(s) 741
PagI TCATGA 1 cut(s) 679
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 239, 747
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 738
PflFI GACNNNGTC 2 cut(s) 262, 388
PinAI ACCGGT 1 cut(s) 843
PkrI GCNGC 6 cut(s) 7, 279, 405, 768, 855, 858
PleI GAGTC 3 cut(s) 253, 379, 872
PpsI GAGTC 3 cut(s) 253, 379, 872
Ppu21I YACGTR 3 cut(s) 229, 340, 466
PshAI GACNNNNGTC 1 cut(s) 675
Psp6I CCWGG 1 cut(s) 522
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 4 cut(s) 79, 219, 330, 456
PspPI GGNCC 3 cut(s) 285, 411, 526
PsuI RGATCY 2 cut(s) 563, 583
PsyI GACNNNGTC 2 cut(s) 262, 388
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 840, 922
SatI GCNGC 6 cut(s) 6, 278, 404, 767, 854, 857
Sau3AI GATC 3 cut(s) 208, 563, 583
Sau96I GGNCC 3 cut(s) 285, 411, 526
SchI GAGTC 3 cut(s) 253, 379, 872
ScrFI CCNGG 5 cut(s) 215, 326, 452, 524, 572
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 1 cut(s) 559
SfuI TTCGAA 1 cut(s) 741
SinI GGWCC 2 cut(s) 285, 411
SmlI CTYRAG 2 cut(s) 533, 860
SmoI CTYRAG 2 cut(s) 533, 860
Sse9I AATT 1 cut(s) 919
SsiI CCGC 5 cut(s) 97, 149, 277, 403, 723
StyD4I CCNGG 5 cut(s) 213, 324, 450, 522, 570
TaiI ACGT 4 cut(s) 169, 231, 342, 468
TaqI TCGA 3 cut(s) 180, 741, 876
TaqII GACCGA 1 cut(s) 611
TasI AATT 1 cut(s) 919
TauI GCSGC 2 cut(s) 280, 406
TfiI GAWTC 1 cut(s) 738
Tru1I TTAA 2 cut(s) 840, 922
Tru9I TTAA 2 cut(s) 840, 922
TseFI GTSAC 2 cut(s) 264, 390
TseI GCWGC 4 cut(s) 5, 766, 853, 856
Tsp45I GTSAC 2 cut(s) 264, 390
TspDTI ATGAA 6 cut(s) 10, 43, 578, 644, 696, 822
TspGWI ACGGA 4 cut(s) 248, 252, 359, 378
Tth111I GACNNNGTC 2 cut(s) 262, 388
VpaK11BI GGWCC 2 cut(s) 285, 411
XmiI GTMKAC 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.