Rh2DG081400

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011769
Physical Location & Seq
Forward (+)
6398238 .. 6399293
1056 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG081400.1

Sequence Viewer

Length: 930 bp
ATGGCAGCTATGTTCGCTTTCATGCTCAGCTGGCTCACACCCACCTCCCTCTTCATCTTCCTCAACCTCGCCATCGGCACCCTCGTCATCTCCCACCGCTTCTGCAAAACACCTCAGCAACAACCCCAACACGACCAGTTCGGTAGACCGCCCTCGCTCCTGGAACGTGTCAAGTCGTTCGACTTCTCCCACTACAACTTCCAACCCGATCACCCGGAACCCCATCACGTAACGGAAAACCCGACCGGATTAGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCAGTACGACTTCCAACATCACTACCCGGAACCCGAACACGTAACGGAACACCCAGCACCGGACAACCCGACCGGATTTGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCAGTACGACTTCCAACAACCCGACCCGGAACCCGAACACGTAACAGAACACCCAACACCGGAAAACCCGACCGGAATAGTCCGGACGCCCTCGCTCCTGGCCCGTCTCAAGTCAATGGATTTCTCCTCTCTCTACAGATCCGACCCGGAAGCTGAAGATCTTCATCCGACAGAGGCCGATAACCGGAACCCGAAACCGAAACCGAAACCGAAGCCGACGCGAGACCATATGGTTCATCGGAGCAAGTCGGACAGGGGTCATGAAGGTTCAGAAATCCGACCCGAAAAGGTGATAAAGAAGTCCGCCAGCGAGAGAGGATTCGAAGAAAACGAGGGAGGTGTCCGACAGCGAAGGGGGGTGGAGAAAACGATGTCGTTTGGAGATGATGAAGAAGTTGACGCGAAAGCTGACGACTTTATTAACCGGTTCAAGCAGCAGCTCAAGTTACAGAGGCTCGACTCGCTTTTGCGTTACAGCAGAAATGCTCCAGAGAAACTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

309

Amino Acids

35.79

Weight (kDa)

6.47

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 4 - 34 1.3e-09 Domain of unknown function (DUF4408)
DUF761 PF05553 271 - 301 2.9e-14 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26130 AT5G56980
fragaria_vesca FvH4_1g07560
malus_domestica MD02G1080400.v1.1 MD15G1207800.v1.1
prunus_persica Prupe.7G209100_v2.0.a1
pyrus_communis pycom15g18410
rosa_laevigata RLG00000016403
rosa_multiflora Rmu_co8329807.1_g000001 Rmu_sc0004086.1_g000010 Rmu_sc0021911.1_g000006
rosa_roxburghii Rroxscaffold_2G00148090
rosa_rugosa Rorug02G0036200 Rorug02G0036300
rosa_samantha Rh2AG082500 Rh2BG083300 Rh2CG085300 Rh2DG081400
rosa_wichuraiana Rw2G006470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 2 cut(s) 277, 403
AccI GTMKAC 1 cut(s) 145
AccII CGCG 2 cut(s) 646, 827
AccIII TCCGGA 3 cut(s) 255, 381, 507
AciI CCGC 5 cut(s) 97, 149, 277, 403, 729
AclWI GGATC 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 2 cut(s) 305, 431
AfiI CCNNNNNNNGG 3 cut(s) 358, 484, 609
AflIII ACRYGT 3 cut(s) 166, 337, 463
AgeI ACCGGT 1 cut(s) 849
AgsI TTSAA 1 cut(s) 856
AjnI CCWGG 2 cut(s) 159, 522
AluBI AGCT 5 cut(s) 8, 30, 578, 833, 865
AluI AGCT 5 cut(s) 8, 30, 578, 833, 865
Alw26I GTCTC 2 cut(s) 536, 642
AlwI GGATC 1 cut(s) 558
Aor13HI TCCGGA 3 cut(s) 255, 381, 507
AoxI GGCC 2 cut(s) 525, 600
ApeKI GCWGC 3 cut(s) 5, 859, 862
AsiGI ACCGGT 1 cut(s) 849
Asp700I GAANNNNTTC 1 cut(s) 585
AspS9I GGNCC 3 cut(s) 285, 411, 526
AsuC2I CCSGG 4 cut(s) 215, 326, 452, 572
AsuHPI GGTGA 2 cut(s) 203, 727
AsuII TTCGAA 1 cut(s) 747
AvaII GGWCC 2 cut(s) 285, 411
BanI GGYRCC 1 cut(s) 77
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 17, 871, 874
BccI CCATC 4 cut(s) 80, 231, 296, 422
BciT130I CCWGG 2 cut(s) 161, 524
BcnI CCSGG 4 cut(s) 215, 326, 452, 572
BcoDI GTCTC 2 cut(s) 536, 642
BfmI CTRYAG 1 cut(s) 559
BglII AGATCT 1 cut(s) 583
BisI GCNGC 5 cut(s) 6, 278, 404, 860, 863
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 5 cut(s) 7, 279, 405, 861, 864
Bme1390I CCNGG 6 cut(s) 161, 215, 326, 452, 524, 572
Bme18I GGWCC 2 cut(s) 285, 411
BmgT120I GGNCC 3 cut(s) 285, 411, 526
BmiI GGNNCC 5 cut(s) 79, 219, 330, 456, 614
BmrFI CCNGG 6 cut(s) 161, 215, 326, 452, 524, 572
BmrI ACTGGG 2 cut(s) 295, 421
BmuI ACTGGG 2 cut(s) 295, 421
BoxI GACNNNNGTC 1 cut(s) 681
BpmI CTGGAG 1 cut(s) 897
Bpu10I CCTNAGC 3 cut(s) 114, 281, 407
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 747
BpuEI CTTGAG 2 cut(s) 518, 851
BpuMI CCSGG 4 cut(s) 215, 326, 452, 572
BsaAI YACGTR 3 cut(s) 229, 340, 466
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 642
Bsc4I CCNNNNNNNGG 3 cut(s) 358, 484, 609
Bse118I RCCGGY 1 cut(s) 849
Bse1I ACTGG 3 cut(s) 136, 301, 427
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 3 cut(s) 255, 381, 507
BseBI CCWGG 2 cut(s) 161, 524
BseGI GGATG 1 cut(s) 589
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 3 cut(s) 358, 484, 609
BseMII CTCAG 4 cut(s) 40, 128, 295, 421
BseNI ACTGG 3 cut(s) 136, 301, 427
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 3 cut(s) 17, 871, 874
BseYI CCCAGC 1 cut(s) 352
Bsh1236I CGCG 2 cut(s) 646, 827
Bsh1285I CGRYCG 3 cut(s) 246, 372, 498
BshFI GGCC 2 cut(s) 527, 602
BshNI GGYRCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 849
BsiEI CGRYCG 3 cut(s) 246, 372, 498
BslI CCNNNNNNNGG 3 cut(s) 358, 484, 609
BsmAI GTCTC 2 cut(s) 536, 642
BsmBI CGTCTC 1 cut(s) 536
BsnI GGCC 2 cut(s) 527, 602
Bso31I GGTCTC 1 cut(s) 642
Bsp119I TTCGAA 1 cut(s) 747
Bsp13I TCCGGA 3 cut(s) 255, 381, 507
Bsp143I GATC 3 cut(s) 208, 563, 583
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 5 cut(s) 97, 149, 277, 403, 729
BspANI GGCC 2 cut(s) 527, 602
BspCNI CTCAG 4 cut(s) 39, 127, 294, 420
BspEI TCCGGA 3 cut(s) 255, 381, 507
BspFNI CGCG 2 cut(s) 646, 827
BspHI TCATGA 1 cut(s) 685
BspLI GGNNCC 5 cut(s) 79, 219, 330, 456, 614
BspPI GGATC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 747
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 642
BsrBI CCGCTC 2 cut(s) 277, 403
BsrFI RCCGGY 1 cut(s) 849
BsrI ACTGG 3 cut(s) 136, 301, 427
BssAI RCCGGY 1 cut(s) 849
BssMI GATC 3 cut(s) 208, 563, 583
BssNI GRCGYC 1 cut(s) 512
Bst2UI CCWGG 2 cut(s) 161, 524
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 512
BstBAI YACGTR 3 cut(s) 229, 340, 466
BstBI TTCGAA 1 cut(s) 747
BstC8I GCNNGC 2 cut(s) 32, 733
BstDEI CTNAG 4 cut(s) 26, 114, 281, 407
BstF5I GGATG 1 cut(s) 589
BstFNI CGCG 2 cut(s) 646, 827
BstKTI GATC 3 cut(s) 211, 566, 586
BstMAI GTCTC 2 cut(s) 536, 642
BstMBI GATC 3 cut(s) 208, 563, 583
BstMCI CGRYCG 3 cut(s) 246, 372, 498
BstMWI GCNNNNNNNGC 3 cut(s) 14, 31, 886
BstNI CCWGG 2 cut(s) 161, 524
BstPAI GACNNNNGTC 1 cut(s) 681
BstSCI CCNGG 6 cut(s) 159, 213, 324, 450, 522, 570
BstSFI CTRYAG 1 cut(s) 559
BstUI CGCG 2 cut(s) 646, 827
BstV1I GCAGC 3 cut(s) 17, 871, 874
BstX2I RGATCY 2 cut(s) 563, 583
BstYI RGATCY 2 cut(s) 563, 583
BsuRI GGCC 2 cut(s) 527, 602
BtsCI GGATG 1 cut(s) 589
Cac8I GCNNGC 2 cut(s) 32, 733
CciI TCATGA 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 849
Cfr13I GGNCC 3 cut(s) 285, 411, 526
CseI GACGC 3 cut(s) 520, 652, 833
Csp6I GTAC 2 cut(s) 304, 430
CspAI ACCGGT 1 cut(s) 849
CviAII CATG 4 cut(s) 22, 271, 397, 686
CviQI GTAC 2 cut(s) 304, 430
DdeI CTNAG 4 cut(s) 26, 114, 281, 407
DpnI GATC 3 cut(s) 210, 565, 585
DpnII GATC 3 cut(s) 208, 563, 583
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 718
Eco31I GGTCTC 1 cut(s) 642
Eco47I GGWCC 2 cut(s) 285, 411
Eco57I CTGAAG 1 cut(s) 600
EcoRII CCWGG 2 cut(s) 159, 522
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 4 cut(s) 25, 274, 400, 689
FaiI YATR 7 cut(s) 11, 23, 272, 398, 654, 656, 687
FatI CATG 4 cut(s) 21, 270, 396, 685
FauNDI CATATG 1 cut(s) 654
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 5 cut(s) 6, 278, 404, 860, 863
FokI GGATG 1 cut(s) 576
Fsp4HI GCNGC 5 cut(s) 6, 278, 404, 860, 863
GluI GCNGC 5 cut(s) 6, 278, 404, 860, 863
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 1 cut(s) 897
HaeIII GGCC 2 cut(s) 527, 602
HgaI GACGC 3 cut(s) 520, 652, 833
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 4 cut(s) 25, 274, 400, 689
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 4 cut(s) 259, 385, 744, 884
HphI GGTGA 2 cut(s) 203, 727
Hpy166II GTNNAC 2 cut(s) 146, 823
Hpy188I TCNGA 7 cut(s) 568, 594, 666, 676, 697, 704, 770
Hpy188III TCNNGA 5 cut(s) 256, 382, 508, 686, 914
Hpy8I GTNNAC 2 cut(s) 146, 823
Hpy99I CGWCG 1 cut(s) 646
HpyAV CCTTC 2 cut(s) 683, 771
HpyCH4IV ACGT 4 cut(s) 166, 228, 339, 465
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 31, 886
HpyF3I CTNAG 4 cut(s) 26, 114, 281, 407
HpySE526I ACGT 4 cut(s) 166, 228, 339, 465
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 4 cut(s) 25, 274, 400, 689
Kpn2I TCCGGA 3 cut(s) 255, 381, 507
Kzo9I GATC 3 cut(s) 208, 563, 583
LmnI GCTCC 6 cut(s) 162, 274, 400, 525, 666, 916
Lsp1109I GCAGC 3 cut(s) 17, 871, 874
MaeII ACGT 4 cut(s) 166, 228, 339, 465
MaeIII GTNAC 7 cut(s) 229, 264, 340, 390, 466, 870, 896
MalI GATC 3 cut(s) 210, 565, 585
MbiI CCGCTC 2 cut(s) 277, 403
MboI GATC 3 cut(s) 208, 563, 583
MboII GAAGA 6 cut(s) 43, 49, 578, 593, 761, 827
MflI RGATCY 2 cut(s) 563, 583
MluCI AATT 1 cut(s) 925
MlyI GAGTC 3 cut(s) 253, 379, 878
MmeI TCCRAC 8 cut(s) 226, 337, 463, 591, 617, 654, 727, 793
MroI TCCGGA 3 cut(s) 255, 381, 507
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 2 cut(s) 846, 928
MspA1I CMGCKG 1 cut(s) 30
MspR9I CCNGG 6 cut(s) 161, 215, 326, 452, 524, 572
MvaI CCWGG 2 cut(s) 161, 524
MvnI CGCG 2 cut(s) 646, 827
MwoI GCNNNNNNNGC 3 cut(s) 14, 31, 886
NciI CCSGG 4 cut(s) 215, 326, 452, 572
NdeI CATATG 1 cut(s) 654
NdeII GATC 3 cut(s) 208, 563, 583
NlaIII CATG 4 cut(s) 25, 274, 400, 689
NlaIV GGNNCC 5 cut(s) 79, 219, 330, 456, 614
NmuCI GTSAC 2 cut(s) 264, 390
NspV TTCGAA 1 cut(s) 747
PagI TCATGA 1 cut(s) 685
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 239, 753
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 744
PflFI GACNNNGTC 2 cut(s) 262, 388
PfoI TCCNGGA 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 849
PkrI GCNGC 5 cut(s) 7, 279, 405, 861, 864
PleI GAGTC 3 cut(s) 253, 379, 878
PpsI GAGTC 3 cut(s) 253, 379, 878
Ppu21I YACGTR 3 cut(s) 229, 340, 466
PshAI GACNNNNGTC 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 159, 522
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 2 cut(s) 159, 522
PspN4I GGNNCC 5 cut(s) 79, 219, 330, 456, 614
PspPI GGNCC 3 cut(s) 285, 411, 526
PsuI RGATCY 2 cut(s) 563, 583
PsyI GACNNNGTC 2 cut(s) 262, 388
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 2 cut(s) 305, 431
RsaNI GTAC 2 cut(s) 304, 430
SaqAI TTAA 2 cut(s) 846, 928
SatI GCNGC 5 cut(s) 6, 278, 404, 860, 863
Sau3AI GATC 3 cut(s) 208, 563, 583
Sau96I GGNCC 3 cut(s) 285, 411, 526
SchI GAGTC 3 cut(s) 253, 379, 878
ScrFI CCNGG 6 cut(s) 161, 215, 326, 452, 524, 572
SfcI CTRYAG 1 cut(s) 559
SfuI TTCGAA 1 cut(s) 747
SinI GGWCC 2 cut(s) 285, 411
SmlI CTYRAG 2 cut(s) 533, 866
SmoI CTYRAG 2 cut(s) 533, 866
Sse9I AATT 1 cut(s) 925
SsiI CCGC 5 cut(s) 97, 149, 277, 403, 729
StyD4I CCNGG 6 cut(s) 159, 213, 324, 450, 522, 570
TaiI ACGT 4 cut(s) 169, 231, 342, 468
TaqI TCGA 3 cut(s) 180, 747, 882
TasI AATT 1 cut(s) 925
TauI GCSGC 2 cut(s) 280, 406
TfiI GAWTC 1 cut(s) 744
Tru1I TTAA 2 cut(s) 846, 928
Tru9I TTAA 2 cut(s) 846, 928
TseFI GTSAC 2 cut(s) 264, 390
TseI GCWGC 3 cut(s) 5, 859, 862
Tsp45I GTSAC 2 cut(s) 264, 390
TspDTI ATGAA 6 cut(s) 10, 43, 578, 650, 702, 828
TspGWI ACGGA 4 cut(s) 248, 252, 359, 378
Tth111I GACNNNGTC 2 cut(s) 262, 388
VpaK11BI GGWCC 2 cut(s) 285, 411
XmiI GTMKAC 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.