RLG00000016403

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0011769
Physical Location & Seq
Forward (+)
6345058 .. 6346098
1041 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016403

Sequence Viewer

Length: 915 bp
ATGGCAGCTATGTTGGCTTTCATCCTCAGCTGGCTCACACCCACCTCCCTCTTCATCTTCCTCAACCTCACCATCGGCACCCTCGTCATCTCCCACCGCTTCTGCAAAACACCTCAGCAACAACCGCAACACGACCAATTCGGTAGACCGCCCTCGCTTCTGGAACGGGTCAGGTCGTTCGACTTTTCCCACTACAACTTCCAACCCGATCACCCGGAACCCCATCACGTAACGGCAAACCCGACCGGATTGGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCACTACAACTTCCAACATCACTACCCGCAACCCGAACACGTAGCAGAATACCCAGCACCGGACAACCCGACCGGAATTGTCCGGACTCCCTCTCTCTTGGACAGGGTCAGGTCCTTCAACTTCTCCCAGTACGACTTCCAACAACCCGATCCGGAACCGGAACACGTAACAGAACACCCAACACCGGAAAACCCGACCGGAATAGTCCGGACGCCCTCGCTGCTGGCCCGTCTCAAGTCAATGGATTTCTCCTCTCTCTACAGATCCGACCCGGAAACTGAAGATCTTCATCCGACCGAGGCCGATAACCCGAAGCCGAAGCCGACGCGAGACCATATGGTTCATCGGAGCAAGTCGGACAGGGGTCATGAAGGTTCGGAAATCCGACCCGAAAAGGTGATAAAGAAGTCCGCCAGCGAGAGAGGATTCGAAGAAAACGAGGGGGGTGTCCGGCAGCGAAGGGGGGTGGAGAAAACGATGTCGTTTGGAGATGATGAAGAAGTTGACGCGAAAGCTGACGACTTCATTAACCGGTTCAAGCAGCAGCTCAAGTTACAGAGGCTCGACTCGCTTTTGCGTCGTTACAACAGAAATGCTCCAGAGAAACTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

305

Amino Acids

35.19

Weight (kDa)

6.6

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 4 - 34 2.4e-09 Domain of unknown function (DUF4408)
DUF761 PF05553 265 - 296 1.6e-13 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26130 AT5G56980
fragaria_vesca FvH4_1g07560
malus_domestica MD02G1080400.v1.1 MD15G1207800.v1.1
prunus_persica Prupe.7G209100_v2.0.a1
pyrus_communis pycom15g18410
rosa_laevigata RLG00000016403
rosa_multiflora Rmu_co8329807.1_g000001 Rmu_sc0004086.1_g000010 Rmu_sc0021911.1_g000006
rosa_roxburghii Rroxscaffold_2G00148090
rosa_rugosa Rorug02G0036200 Rorug02G0036300
rosa_samantha Rh2AG082500 Rh2BG083300 Rh2CG085300 Rh2DG081400
rosa_wichuraiana Rw2G006470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 277
AccI GTMKAC 1 cut(s) 145
AccII CGCG 2 cut(s) 628, 809
AccIII TCCGGA 4 cut(s) 255, 381, 451, 507
AciI CCGC 6 cut(s) 97, 125, 149, 277, 326, 711
AclWI GGATC 2 cut(s) 443, 558
AcuI CTGAAG 1 cut(s) 600
AcyI GRCGYC 1 cut(s) 512
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 358, 484
AflIII ACRYGT 2 cut(s) 337, 463
AgeI ACCGGT 1 cut(s) 831
AgsI TTSAA 2 cut(s) 418, 838
AluBI AGCT 4 cut(s) 8, 30, 815, 847
AluI AGCT 4 cut(s) 8, 30, 815, 847
Alw26I GTCTC 2 cut(s) 536, 624
AlwI GGATC 2 cut(s) 443, 558
Aor13HI TCCGGA 4 cut(s) 255, 381, 451, 507
AoxI GGCC 2 cut(s) 525, 600
ApeKI GCWGC 5 cut(s) 5, 520, 754, 841, 844
AsiGI ACCGGT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 585
AspS9I GGNCC 4 cut(s) 253, 285, 411, 526
AsuC2I CCSGG 2 cut(s) 215, 572
AsuHPI GGTGA 3 cut(s) 61, 203, 709
AsuII TTCGAA 1 cut(s) 729
AvaII GGWCC 3 cut(s) 253, 285, 411
BanI GGYRCC 1 cut(s) 77
BbvCI CCTCAGC 2 cut(s) 26, 114
BbvI GCAGC 5 cut(s) 17, 507, 766, 853, 856
BccI CCATC 3 cut(s) 80, 231, 296
BceAI ACGGC 1 cut(s) 249
BcnI CCSGG 2 cut(s) 215, 572
BcoDI GTCTC 2 cut(s) 536, 624
BfmI CTRYAG 1 cut(s) 559
BglII AGATCT 1 cut(s) 583
BisI GCNGC 6 cut(s) 6, 278, 521, 755, 842, 845
BlsI GCNGC 6 cut(s) 7, 279, 522, 756, 843, 846
Bme1390I CCNGG 2 cut(s) 215, 572
Bme18I GGWCC 3 cut(s) 253, 285, 411
BmgT120I GGNCC 4 cut(s) 253, 285, 411, 526
BmiI GGNNCC 3 cut(s) 79, 219, 456
BmrFI CCNGG 2 cut(s) 215, 572
BmrI ACTGGG 1 cut(s) 421
BmuI ACTGGG 1 cut(s) 421
BoxI GACNNNNGTC 1 cut(s) 663
BpmI CTGGAG 1 cut(s) 882
Bpu10I CCTNAGC 3 cut(s) 26, 114, 281
Bpu14I TTCGAA 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 518, 833
BpuMI CCSGG 2 cut(s) 215, 572
BsaAI YACGTR 3 cut(s) 229, 340, 466
BsaBI GATNNNNATC 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 624
BsaJI CCNNGG 1 cut(s) 597
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 484
Bse118I RCCGGY 1 cut(s) 831
Bse1I ACTGG 1 cut(s) 427
Bse8I GATNNNNATC 1 cut(s) 588
BseAI TCCGGA 4 cut(s) 255, 381, 451, 507
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 2 cut(s) 21, 589
BseJI GATNNNNATC 1 cut(s) 588
BseLI CCNNNNNNNGG 2 cut(s) 358, 484
BseMII CTCAG 3 cut(s) 40, 128, 295
BseNI ACTGG 1 cut(s) 427
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 5 cut(s) 17, 507, 766, 853, 856
BseYI CCCAGC 1 cut(s) 352
Bsh1236I CGCG 2 cut(s) 628, 809
Bsh1285I CGRYCG 4 cut(s) 246, 372, 498, 597
BshFI GGCC 2 cut(s) 527, 602
BshNI GGYRCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 831
BsiEI CGRYCG 4 cut(s) 246, 372, 498, 597
BslI CCNNNNNNNGG 2 cut(s) 358, 484
BsmAI GTCTC 2 cut(s) 536, 624
BsmBI CGTCTC 1 cut(s) 536
BsnI GGCC 2 cut(s) 527, 602
Bso31I GGTCTC 1 cut(s) 624
Bsp119I TTCGAA 1 cut(s) 729
Bsp13I TCCGGA 4 cut(s) 255, 381, 451, 507
Bsp143I GATC 4 cut(s) 208, 448, 563, 583
BspACI CCGC 6 cut(s) 97, 125, 149, 277, 326, 711
BspANI GGCC 2 cut(s) 527, 602
BspCNI CTCAG 3 cut(s) 39, 127, 294
BspEI TCCGGA 4 cut(s) 255, 381, 451, 507
BspFNI CGCG 2 cut(s) 628, 809
BspHI TCATGA 1 cut(s) 667
BspLI GGNNCC 3 cut(s) 79, 219, 456
BspPI GGATC 2 cut(s) 443, 558
BspT104I TTCGAA 1 cut(s) 729
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 624
BsrBI CCGCTC 1 cut(s) 277
BsrFI RCCGGY 1 cut(s) 831
BsrI ACTGG 1 cut(s) 427
BssAI RCCGGY 1 cut(s) 831
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 4 cut(s) 208, 448, 563, 583
BssNI GRCGYC 1 cut(s) 512
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 512
BstBAI YACGTR 3 cut(s) 229, 340, 466
BstBI TTCGAA 1 cut(s) 729
BstC8I GCNNGC 3 cut(s) 32, 525, 715
BstDEI CTNAG 3 cut(s) 26, 114, 281
BstF5I GGATG 2 cut(s) 21, 589
BstFNI CGCG 2 cut(s) 628, 809
BstKTI GATC 4 cut(s) 211, 451, 566, 586
BstMAI GTCTC 2 cut(s) 536, 624
BstMBI GATC 4 cut(s) 208, 448, 563, 583
BstMCI CGRYCG 4 cut(s) 246, 372, 498, 597
BstMWI GCNNNNNNNGC 4 cut(s) 14, 124, 520, 868
BstPAI GACNNNNGTC 1 cut(s) 663
BstSCI CCNGG 2 cut(s) 213, 570
BstSFI CTRYAG 1 cut(s) 559
BstUI CGCG 2 cut(s) 628, 809
BstV1I GCAGC 5 cut(s) 17, 507, 766, 853, 856
BstX2I RGATCY 2 cut(s) 563, 583
BstYI RGATCY 2 cut(s) 563, 583
BsuRI GGCC 2 cut(s) 527, 602
BtsCI GGATG 2 cut(s) 21, 589
Cac8I GCNNGC 3 cut(s) 32, 525, 715
CciI TCATGA 1 cut(s) 667
Cfr10I RCCGGY 1 cut(s) 831
Cfr13I GGNCC 4 cut(s) 253, 285, 411, 526
CseI GACGC 4 cut(s) 520, 634, 815, 866
Csp6I GTAC 1 cut(s) 430
CspAI ACCGGT 1 cut(s) 831
CviAII CATG 2 cut(s) 271, 668
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 3 cut(s) 26, 114, 281
DpnI GATC 4 cut(s) 210, 450, 565, 585
DpnII GATC 4 cut(s) 208, 448, 563, 583
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 700
Eco31I GGTCTC 1 cut(s) 624
Eco47I GGWCC 3 cut(s) 253, 285, 411
Eco57I CTGAAG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 411
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 2 cut(s) 274, 671
FaiI YATR 5 cut(s) 11, 272, 636, 638, 669
FatI CATG 2 cut(s) 270, 667
FauI CCCGC 1 cut(s) 333
FauNDI CATATG 1 cut(s) 636
FblI GTMKAC 1 cut(s) 145
Fnu4HI GCNGC 6 cut(s) 6, 278, 521, 755, 842, 845
FokI GGATG 2 cut(s) 8, 576
Fsp4HI GCNGC 6 cut(s) 6, 278, 521, 755, 842, 845
GluI GCNGC 6 cut(s) 6, 278, 521, 755, 842, 845
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 1 cut(s) 882
HaeIII GGCC 2 cut(s) 527, 602
HgaI GACGC 4 cut(s) 520, 634, 815, 866
Hin1I GRCGYC 1 cut(s) 512
Hin1II CATG 2 cut(s) 274, 671
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HinfI GANTC 4 cut(s) 259, 385, 726, 866
HphI GGTGA 3 cut(s) 61, 203, 709
Hpy166II GTNNAC 2 cut(s) 146, 805
Hpy188I TCNGA 6 cut(s) 568, 594, 648, 658, 679, 686
Hpy188III TCNNGA 7 cut(s) 161, 256, 382, 452, 508, 668, 899
Hpy8I GTNNAC 2 cut(s) 146, 805
Hpy99I CGWCG 2 cut(s) 628, 882
HpyAV CCTTC 3 cut(s) 424, 665, 753
HpyCH4IV ACGT 3 cut(s) 228, 339, 465
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 124, 520, 868
HpyF3I CTNAG 3 cut(s) 26, 114, 281
HpySE526I ACGT 3 cut(s) 228, 339, 465
Hsp92I GRCGYC 1 cut(s) 512
Hsp92II CATG 2 cut(s) 274, 671
Kpn2I TCCGGA 4 cut(s) 255, 381, 451, 507
Kzo9I GATC 4 cut(s) 208, 448, 563, 583
LmnI GCTCC 3 cut(s) 274, 648, 901
Lsp1109I GCAGC 5 cut(s) 17, 507, 766, 853, 856
MaeII ACGT 3 cut(s) 228, 339, 465
MaeIII GTNAC 5 cut(s) 229, 264, 466, 852, 881
MalI GATC 4 cut(s) 210, 450, 565, 585
MbiI CCGCTC 1 cut(s) 277
MboI GATC 4 cut(s) 208, 448, 563, 583
MboII GAAGA 6 cut(s) 43, 49, 578, 593, 743, 809
MflI RGATCY 2 cut(s) 563, 583
MluCI AATT 3 cut(s) 137, 375, 910
MlyI GAGTC 3 cut(s) 253, 379, 860
MmeI TCCRAC 7 cut(s) 226, 337, 463, 591, 617, 636, 709
MroI TCCGGA 4 cut(s) 255, 381, 451, 507
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 2 cut(s) 828, 913
MspA1I CMGCKG 1 cut(s) 30
MspR9I CCNGG 2 cut(s) 215, 572
MvnI CGCG 2 cut(s) 628, 809
MwoI GCNNNNNNNGC 4 cut(s) 14, 124, 520, 868
NciI CCSGG 2 cut(s) 215, 572
NdeI CATATG 1 cut(s) 636
NdeII GATC 4 cut(s) 208, 448, 563, 583
NlaIII CATG 2 cut(s) 274, 671
NlaIV GGNNCC 3 cut(s) 79, 219, 456
NmuCI GTSAC 1 cut(s) 264
NspV TTCGAA 1 cut(s) 729
PagI TCATGA 1 cut(s) 667
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 239, 735
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 1 cut(s) 726
PflFI GACNNNGTC 2 cut(s) 262, 404
PinAI ACCGGT 1 cut(s) 831
PkrI GCNGC 6 cut(s) 7, 279, 522, 756, 843, 846
PleI GAGTC 3 cut(s) 253, 379, 860
PpsI GAGTC 3 cut(s) 253, 379, 860
Ppu21I YACGTR 3 cut(s) 229, 340, 466
PpuMI RGGWCCY 1 cut(s) 411
PshAI GACNNNNGTC 1 cut(s) 663
Psp5II RGGWCCY 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 352
PspN4I GGNNCC 3 cut(s) 79, 219, 456
PspPI GGNCC 4 cut(s) 253, 285, 411, 526
PspPPI RGGWCCY 1 cut(s) 411
PsuI RGATCY 2 cut(s) 563, 583
PsyI GACNNNGTC 2 cut(s) 262, 404
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
SaqAI TTAA 2 cut(s) 828, 913
SatI GCNGC 6 cut(s) 6, 278, 521, 755, 842, 845
Sau3AI GATC 4 cut(s) 208, 448, 563, 583
Sau96I GGNCC 4 cut(s) 253, 285, 411, 526
SchI GAGTC 3 cut(s) 253, 379, 860
ScrFI CCNGG 2 cut(s) 215, 572
SfcI CTRYAG 1 cut(s) 559
SfuI TTCGAA 1 cut(s) 729
SinI GGWCC 3 cut(s) 253, 285, 411
SmlI CTYRAG 2 cut(s) 533, 848
SmoI CTYRAG 2 cut(s) 533, 848
Sse9I AATT 3 cut(s) 137, 375, 910
SsiI CCGC 6 cut(s) 97, 125, 149, 277, 326, 711
StyD4I CCNGG 2 cut(s) 213, 570
TaiI ACGT 3 cut(s) 231, 342, 468
TaqI TCGA 3 cut(s) 180, 729, 864
TaqII GACCGA 1 cut(s) 611
TasI AATT 3 cut(s) 137, 375, 910
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 726
Tru1I TTAA 2 cut(s) 828, 913
Tru9I TTAA 2 cut(s) 828, 913
TseFI GTSAC 1 cut(s) 264
TseI GCWGC 5 cut(s) 5, 520, 754, 841, 844
Tsp45I GTSAC 1 cut(s) 264
TspDTI ATGAA 7 cut(s) 10, 43, 578, 632, 684, 810, 814
TspGWI ACGGA 1 cut(s) 252
Tth111I GACNNNGTC 2 cut(s) 262, 404
VpaK11BI GGWCC 3 cut(s) 253, 285, 411
XmiI GTMKAC 1 cut(s) 145
XmnI GAANNNNTTC 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.