Rroxscaffold_2G00148090

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011769
Physical Location & Seq
Reverse (-)
85735099 .. 85736133
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00148090.1

Sequence Viewer

Length: 1035 bp
ATGGCAGCTATGTTGGCTTTCATGCTCAGCTGGCTCACACCCACCTCCATCTTCATCTTCCTCAACCTCGCCATCGGCACCCTCGTCATCTCCCACCGCTTCTGCAAAACACCTCAGCAACACGACCAATTCGGTAGACCGCCCTCGCTCCTGGAACGGGTCAGGTCGTTCGACTTCTCCCACTACAACTTCCAACCCGATCACCCGGAACCCCATCACGTAGCGGAAAACCCGACCGGATTAGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCACTACAACTTCCAACATCACTACCCGGAACCCGAACACGTAACGGAATACCCAGCACCGGACAACCCGACCGGATTAGTCCGGACTCCGTCACTCATGGAGCGGCTCAGGTCCATCAACTTTTCCCACTACAACTTCCAACATCACTACCCGCAACCCGAACACGTAGCAGAATACCCAGAACCGGACCACCCGACCGGAATTGTCCGGACTCCCTCGCTCTTGGACAGGGTCATGTCCTTCAACTTCTCCCAGTACGACTTCCAACAACCCGACCCGGAACCGGAACACGTAACAGAACACCCAACACCGGAAAACCCGACCGGAATAGTCCGGACGCCCTCGCTTCTGGCCCGTCTCAAGTCAATGGATTTCTCCTCTCTCTACAGATCCGACCCGGAAGCTGAAGATCTTCATCCGACCGAGGCCGATAACCCGAACCAGAAACCGAAACCGAAGCCTACGCGAGACCATACGGTTCATCGGAGCAAGTCGGACAGGGGTCATGAAGGTTCGGAAATCCGACCCGAAAAGGTGATAAAGAAGTCCGCCAGCGAGAGAGGATTCGAAGAAAACGAGGGGGGTGTCCGGCAGCCAAGGGGGTGGACAAAACGATGTCGTTTGGAGATGATGAAGAAGTTGACGCGAAAGCTGACGACTTCATTAACCGGTTCAAGCAGCAGCTCAAGTTACAGAGGCTCGACTCGCTTTTGCGTTACAAAAGAAATGCTCCAGAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

344

Amino Acids

39.74

Weight (kDa)

7.88

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 4 - 34 2e-08 Domain of unknown function (DUF4408)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011769)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26130 AT5G56980
fragaria_vesca FvH4_1g07560
malus_domestica MD02G1080400.v1.1 MD15G1207800.v1.1
prunus_persica Prupe.7G209100_v2.0.a1
pyrus_communis pycom15g18410
rosa_laevigata RLG00000016403
rosa_multiflora Rmu_co8329807.1_g000001 Rmu_sc0004086.1_g000010 Rmu_sc0021911.1_g000006
rosa_roxburghii Rroxscaffold_2G00148090
rosa_rugosa Rorug02G0036200 Rorug02G0036300
rosa_samantha Rh2AG082500 Rh2BG083300 Rh2CG085300 Rh2DG081400
rosa_wichuraiana Rw2G006470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 2 cut(s) 268, 394
AccI GTMKAC 1 cut(s) 136
AccII CGCG 2 cut(s) 757, 937
AccIII TCCGGA 4 cut(s) 246, 372, 498, 624
AciI CCGC 7 cut(s) 97, 140, 224, 268, 394, 443, 840
AclWI GGATC 1 cut(s) 675
AcuI CTGAAG 1 cut(s) 717
AcyI GRCGYC 1 cut(s) 629
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 5 cut(s) 157, 349, 475, 574, 601
AflIII ACRYGT 3 cut(s) 328, 454, 580
AgeI ACCGGT 1 cut(s) 959
AgsI TTSAA 2 cut(s) 535, 966
AjnI CCWGG 1 cut(s) 150
AluBI AGCT 5 cut(s) 8, 30, 695, 943, 975
AluI AGCT 5 cut(s) 8, 30, 695, 943, 975
Alw26I GTCTC 2 cut(s) 653, 753
AlwI GGATC 1 cut(s) 675
Aor13HI TCCGGA 4 cut(s) 246, 372, 498, 624
AoxI GGCC 2 cut(s) 642, 717
ApeKI GCWGC 4 cut(s) 5, 883, 969, 972
AsiGI ACCGGT 1 cut(s) 959
Asp700I GAANNNNTTC 1 cut(s) 702
AspS9I GGNCC 4 cut(s) 276, 402, 478, 643
AsuC2I CCSGG 4 cut(s) 206, 317, 569, 689
AsuHPI GGTGA 2 cut(s) 194, 838
AsuII TTCGAA 1 cut(s) 858
AvaII GGWCC 3 cut(s) 276, 402, 478
BanI GGYRCC 1 cut(s) 77
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 4 cut(s) 17, 895, 981, 984
BccI CCATC 5 cut(s) 56, 80, 222, 287, 413
BciT130I CCWGG 1 cut(s) 152
BcnI CCSGG 4 cut(s) 206, 317, 569, 689
BcoDI GTCTC 2 cut(s) 653, 753
BfmI CTRYAG 1 cut(s) 676
BglII AGATCT 1 cut(s) 700
BisI GCNGC 6 cut(s) 6, 269, 395, 884, 970, 973
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 6 cut(s) 7, 270, 396, 885, 971, 974
Bme1390I CCNGG 5 cut(s) 152, 206, 317, 569, 689
Bme18I GGWCC 3 cut(s) 276, 402, 478
BmgT120I GGNCC 4 cut(s) 276, 402, 478, 643
BmiI GGNNCC 4 cut(s) 79, 210, 321, 573
BmrFI CCNGG 5 cut(s) 152, 206, 317, 569, 689
BmrI ACTGGG 1 cut(s) 538
BmuI ACTGGG 1 cut(s) 538
BoxI GACNNNNGTC 1 cut(s) 792
BpmI CTGGAG 1 cut(s) 1007
Bpu10I CCTNAGC 3 cut(s) 114, 272, 398
Bpu1102I GCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 858
BpuEI CTTGAG 2 cut(s) 635, 961
BpuMI CCSGG 4 cut(s) 206, 317, 569, 689
BsaAI YACGTR 4 cut(s) 220, 331, 457, 583
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 629
BsaI GGTCTC 1 cut(s) 753
BsaJI CCNNGG 2 cut(s) 714, 887
Bsc4I CCNNNNNNNGG 5 cut(s) 157, 349, 475, 574, 601
Bse118I RCCGGY 1 cut(s) 959
Bse1I ACTGG 1 cut(s) 544
Bse8I GATNNNNATC 1 cut(s) 705
BseAI TCCGGA 4 cut(s) 246, 372, 498, 624
BseBI CCWGG 1 cut(s) 152
BseDI CCNNGG 2 cut(s) 714, 887
BseGI GGATG 1 cut(s) 706
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 5 cut(s) 157, 349, 475, 574, 601
BseMII CTCAG 4 cut(s) 40, 128, 286, 412
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 658
BseXI GCAGC 4 cut(s) 17, 895, 981, 984
BseYI CCCAGC 1 cut(s) 343
Bsh1236I CGCG 2 cut(s) 757, 937
Bsh1285I CGRYCG 5 cut(s) 237, 363, 489, 615, 714
BshFI GGCC 2 cut(s) 644, 719
BshNI GGYRCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 959
BsiEI CGRYCG 5 cut(s) 237, 363, 489, 615, 714
BslI CCNNNNNNNGG 5 cut(s) 157, 349, 475, 574, 601
BsmAI GTCTC 2 cut(s) 653, 753
BsmBI CGTCTC 1 cut(s) 653
BsnI GGCC 2 cut(s) 644, 719
Bso31I GGTCTC 1 cut(s) 753
Bsp119I TTCGAA 1 cut(s) 858
Bsp13I TCCGGA 4 cut(s) 246, 372, 498, 624
Bsp143I GATC 3 cut(s) 199, 680, 700
Bsp1720I GCTNAGC 1 cut(s) 26
BspACI CCGC 7 cut(s) 97, 140, 224, 268, 394, 443, 840
BspANI GGCC 2 cut(s) 644, 719
BspCNI CTCAG 4 cut(s) 39, 127, 285, 411
BspEI TCCGGA 4 cut(s) 246, 372, 498, 624
BspFNI CGCG 2 cut(s) 757, 937
BspHI TCATGA 1 cut(s) 796
BspLI GGNNCC 4 cut(s) 79, 210, 321, 573
BspPI GGATC 1 cut(s) 675
BspT104I TTCGAA 1 cut(s) 858
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 753
BsrBI CCGCTC 2 cut(s) 268, 394
BsrFI RCCGGY 1 cut(s) 959
BsrI ACTGG 1 cut(s) 544
BssAI RCCGGY 1 cut(s) 959
BssECI CCNNGG 2 cut(s) 714, 887
BssMI GATC 3 cut(s) 199, 680, 700
BssNI GRCGYC 1 cut(s) 629
BssT1I CCWWGG 1 cut(s) 887
Bst2UI CCWGG 1 cut(s) 152
Bst4CI ACNGT 1 cut(s) 769
BstACI GRCGYC 1 cut(s) 629
BstBAI YACGTR 4 cut(s) 220, 331, 457, 583
BstBI TTCGAA 1 cut(s) 858
BstC8I GCNNGC 2 cut(s) 32, 844
BstDEI CTNAG 4 cut(s) 26, 114, 272, 398
BstF5I GGATG 1 cut(s) 706
BstFNI CGCG 2 cut(s) 757, 937
BstKTI GATC 3 cut(s) 202, 683, 703
BstMAI GTCTC 2 cut(s) 653, 753
BstMBI GATC 3 cut(s) 199, 680, 700
BstMCI CGRYCG 5 cut(s) 237, 363, 489, 615, 714
BstMWI GCNNNNNNNGC 3 cut(s) 14, 31, 996
BstNI CCWGG 1 cut(s) 152
BstPAI GACNNNNGTC 1 cut(s) 792
BstSCI CCNGG 5 cut(s) 150, 204, 315, 567, 687
BstSFI CTRYAG 1 cut(s) 676
BstUI CGCG 2 cut(s) 757, 937
BstV1I GCAGC 4 cut(s) 17, 895, 981, 984
BstX2I RGATCY 2 cut(s) 680, 700
BstXI CCANNNNNNTGG 1 cut(s) 894
BstYI RGATCY 2 cut(s) 680, 700
BsuRI GGCC 2 cut(s) 644, 719
BtsCI GGATG 1 cut(s) 706
Cac8I GCNNGC 2 cut(s) 32, 844
CciI TCATGA 1 cut(s) 796
Cfr10I RCCGGY 1 cut(s) 959
Cfr13I GGNCC 4 cut(s) 276, 402, 478, 643
CseI GACGC 2 cut(s) 637, 943
Csp6I GTAC 1 cut(s) 547
CspAI ACCGGT 1 cut(s) 959
CviAII CATG 5 cut(s) 22, 262, 388, 526, 797
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 4 cut(s) 26, 114, 272, 398
DpnI GATC 3 cut(s) 201, 682, 702
DpnII GATC 3 cut(s) 199, 680, 700
EciI GGCGGA 1 cut(s) 829
Eco130I CCWWGG 1 cut(s) 887
Eco31I GGTCTC 1 cut(s) 753
Eco47I GGWCC 3 cut(s) 276, 402, 478
Eco57I CTGAAG 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 150
EcoT14I CCWWGG 1 cut(s) 887
ErhI CCWWGG 1 cut(s) 887
Esp3I CGTCTC 1 cut(s) 653
FaeI CATG 5 cut(s) 25, 265, 391, 529, 800
FaiI YATR 7 cut(s) 11, 23, 263, 389, 527, 765, 798
FatI CATG 5 cut(s) 21, 261, 387, 525, 796
FauI CCCGC 1 cut(s) 450
FblI GTMKAC 1 cut(s) 136
Fnu4HI GCNGC 6 cut(s) 6, 269, 395, 884, 970, 973
FokI GGATG 1 cut(s) 693
Fsp4HI GCNGC 6 cut(s) 6, 269, 395, 884, 970, 973
GluI GCNGC 6 cut(s) 6, 269, 395, 884, 970, 973
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 1007
HaeIII GGCC 2 cut(s) 644, 719
HgaI GACGC 2 cut(s) 637, 943
Hin1I GRCGYC 1 cut(s) 629
Hin1II CATG 5 cut(s) 25, 265, 391, 529, 800
HincII GTYRAC 1 cut(s) 933
HindII GTYRAC 1 cut(s) 933
HinfI GANTC 5 cut(s) 250, 376, 502, 855, 994
HphI GGTGA 2 cut(s) 194, 838
Hpy166II GTNNAC 3 cut(s) 137, 897, 933
Hpy188I TCNGA 6 cut(s) 685, 711, 777, 787, 808, 815
Hpy188III TCNNGA 6 cut(s) 247, 373, 499, 625, 797, 1024
Hpy8I GTNNAC 3 cut(s) 137, 897, 933
HpyAV CCTTC 2 cut(s) 541, 794
HpyCH4III ACNGT 1 cut(s) 769
HpyCH4IV ACGT 4 cut(s) 219, 330, 456, 582
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 31, 996
HpyF3I CTNAG 4 cut(s) 26, 114, 272, 398
HpySE526I ACGT 4 cut(s) 219, 330, 456, 582
Hsp92I GRCGYC 1 cut(s) 629
Hsp92II CATG 5 cut(s) 25, 265, 391, 529, 800
Kpn2I TCCGGA 4 cut(s) 246, 372, 498, 624
Kzo9I GATC 3 cut(s) 199, 680, 700
LmnI GCTCC 5 cut(s) 153, 265, 391, 777, 1026
Lsp1109I GCAGC 4 cut(s) 17, 895, 981, 984
MaeII ACGT 4 cut(s) 219, 330, 456, 582
MaeIII GTNAC 6 cut(s) 255, 331, 381, 583, 980, 1006
MalI GATC 3 cut(s) 201, 682, 702
MbiI CCGCTC 2 cut(s) 268, 394
MboI GATC 3 cut(s) 199, 680, 700
MboII GAAGA 6 cut(s) 43, 49, 695, 710, 872, 937
MflI RGATCY 2 cut(s) 680, 700
MluCI AATT 2 cut(s) 128, 492
MlyI GAGTC 4 cut(s) 244, 370, 496, 988
MmeI TCCRAC 8 cut(s) 217, 328, 454, 580, 708, 734, 765, 838
MroI TCCGGA 4 cut(s) 246, 372, 498, 624
MroXI GAANNNNTTC 1 cut(s) 702
MseI TTAA 1 cut(s) 956
MspA1I CMGCKG 1 cut(s) 30
MspR9I CCNGG 5 cut(s) 152, 206, 317, 569, 689
MvaI CCWGG 1 cut(s) 152
MvnI CGCG 2 cut(s) 757, 937
MwoI GCNNNNNNNGC 3 cut(s) 14, 31, 996
NciI CCSGG 4 cut(s) 206, 317, 569, 689
NdeII GATC 3 cut(s) 199, 680, 700
NlaIII CATG 5 cut(s) 25, 265, 391, 529, 800
NlaIV GGNNCC 4 cut(s) 79, 210, 321, 573
NmuCI GTSAC 2 cut(s) 255, 381
NspV TTCGAA 1 cut(s) 858
PagI TCATGA 1 cut(s) 796
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 864
PdmI GAANNNNTTC 1 cut(s) 702
PfeI GAWTC 1 cut(s) 855
PflFI GACNNNGTC 3 cut(s) 253, 379, 521
PfoI TCCNGGA 1 cut(s) 150
PinAI ACCGGT 1 cut(s) 959
PkrI GCNGC 6 cut(s) 7, 270, 396, 885, 971, 974
PleI GAGTC 4 cut(s) 244, 370, 496, 988
PpsI GAGTC 4 cut(s) 244, 370, 496, 988
Ppu21I YACGTR 4 cut(s) 220, 331, 457, 583
PshAI GACNNNNGTC 1 cut(s) 792
Psp6I CCWGG 1 cut(s) 150
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 1 cut(s) 150
PspN4I GGNNCC 4 cut(s) 79, 210, 321, 573
PspPI GGNCC 4 cut(s) 276, 402, 478, 643
PsuI RGATCY 2 cut(s) 680, 700
PsyI GACNNNGTC 3 cut(s) 253, 379, 521
PvuII CAGCTG 1 cut(s) 30
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
SaqAI TTAA 1 cut(s) 956
SatI GCNGC 6 cut(s) 6, 269, 395, 884, 970, 973
Sau3AI GATC 3 cut(s) 199, 680, 700
Sau96I GGNCC 4 cut(s) 276, 402, 478, 643
SchI GAGTC 4 cut(s) 244, 370, 496, 988
ScrFI CCNGG 5 cut(s) 152, 206, 317, 569, 689
SfcI CTRYAG 1 cut(s) 676
SfuI TTCGAA 1 cut(s) 858
SinI GGWCC 3 cut(s) 276, 402, 478
SmlI CTYRAG 2 cut(s) 650, 976
SmoI CTYRAG 2 cut(s) 650, 976
Sse9I AATT 2 cut(s) 128, 492
SsiI CCGC 7 cut(s) 97, 140, 224, 268, 394, 443, 840
StyD4I CCNGG 5 cut(s) 150, 204, 315, 567, 687
StyI CCWWGG 1 cut(s) 887
TaaI ACNGT 1 cut(s) 769
TaiI ACGT 4 cut(s) 222, 333, 459, 585
TaqI TCGA 3 cut(s) 171, 858, 992
TaqII GACCGA 1 cut(s) 728
TasI AATT 2 cut(s) 128, 492
TauI GCSGC 2 cut(s) 271, 397
TfiI GAWTC 1 cut(s) 855
Tru1I TTAA 1 cut(s) 956
Tru9I TTAA 1 cut(s) 956
TseFI GTSAC 2 cut(s) 255, 381
TseI GCWGC 4 cut(s) 5, 883, 969, 972
Tsp45I GTSAC 2 cut(s) 255, 381
TspDTI ATGAA 7 cut(s) 10, 43, 695, 761, 813, 938, 942
TspGWI ACGGA 3 cut(s) 243, 350, 369
Tth111I GACNNNGTC 3 cut(s) 253, 379, 521
VpaK11BI GGWCC 3 cut(s) 276, 402, 478
XmiI GTMKAC 1 cut(s) 136
XmnI GAANNNNTTC 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.