Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003985

Physical Location & Seq

Genomic Coordinates Reverse (-)

8754196 .. 8758023

Gene Structure 3828 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1108700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGGCAGTTCGCCGGCGACCCCCAACCGCCTCTGCAGCCGGCGAACCCATTAAATCCGACAACCAGCTGCTCAAGGCCTCCGATGCGCTTCCCCTGCCGCTTTACCTCACCAATGGCCTCTTCTTCACCCTCTTCTTCGCCGTCGCCTACTTCCTCCTCAACCGGTGGCGCGAGAAGATCCGCAGCTCCACGCCGCTGCACGTCGTCACGCTGTCCGAGCTCGCCGCCCTCTTCTCCCTGTTCGCCTCCATCATCTACCTCCTCGGCTTCTTCGGCATTGACTTCGTCCAGTCCTTCGTTTCCCCCAACTCCAGCGCCGCCCACGGCCCCTGGAACGATGACGTCGTAGTCCGGCAAAATAGTTCTATCAATTTAACCGCACTCCCCTCCTCCGCCACCTCCGCCGCCTACGAGTCGCCGGCGTTGTCGGCTGATGACGAGGAAATCATCAAGTCTGTCGTCGATGGAACTACGCCTTCGTACTCTCTCGAGTCCACCCTCGGAGACTGCCGGAGGGCGGCTGCGATACGGCGCGAGGCCCTGCAGCGGACGACCGGTAGTTCGCTGGATGGCCTCCCGCTGGAAGGGTTCGACTACGAGGCGATACTGGGGCAATGCTGCGAGATGCCGGTGGGGTACGTGCAGATCCCGGTGGGAGTGGTGGGGCCGCTGCTGATCGACGGGGAGGAGTACACGGTGCCAATGGCGACCACGGAGGGGTGCTTGGTGGCCAGTACCAATAGGGGATGCAAGGCCATCCTCATGTCCGGCGGGGCCCGCAGCGTGGTTCTCAAGGACGGTATGACCCGAGCTCCCGTTGTCCGGTTCAGCTCAGCCGCCAGAGCGTCCGAACTCAAGTTCTTCGTTGAGGACCTGCTCAACTTTGATACACTCGCCGTCGTTTTCAACCGATCAAGTAGATTTGCGAAGCTTCAGAGGATTCAATGCTCGATAGCAGGGAAGAATGTGTACATGAGGTTTACTTGCAGCACTGGAGATGCTATGGGGATGAACATGGTCTCCAAAGGCGTCCAAAACGTTCTAGATTTCCTCCAAAGCGACTTCCCTGACATGGATATTATTGGCATCTCTGGAAACTTCTGCTCCGACAAGAAACCGGCGGCGGTGAATTGGATAGAAGGGAGAGGAAAGTCAGTGGTGTGTGAGGCAGTGATCAAGGAAGAGGTGGTGAGGAAGGTACTGAAGACAAATGTGGCTGCCTTGGTGGAGCTCAACATGCTTAAGAACCTTACTGGTTCTGCATTGGCCGGTGCTCTTGGTGGGTTCAATGCGCACGCCAGCAACATTGTCGCCGCGATCTATATCGCCACCGGCCAAGATCCGGCTCAGAATGTTGAGAGCTCTCACTGCATCACCATGATGGAAGCCATAAACGACGGCAAGGACCTTCATGTCTCCGTCACCATGCCTTCCATTGAGGTGGGAACGGTTGGAGGAGGAACCCAGCTTGCATCACAAGCAGCCTGCCTGAACCTGCTGGGTGTGAAGGGTGCAAGCAGAGACTCGCCCGGTTCAAACTCTAGAAAATTGGCCACCATTGTCGCGGGCTCAGTGCTCGCCGGAGAACTTTCGCTGATGTCTGCAATTGCAGCGGGACAGCTAGTCAAGAGCCACATGAAATACAACAGATCTAGCAAGGATGTCTCAAAACTTGTCTGCCCCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004420 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006720 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016125 GO:0016126 GO:0016491 GO:0016614 GO:0016616 GO:0031090 GO:0031903 GO:0031984 GO:0042175 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098588 GO:0098805 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 ko04152 ko04976 map00900 map01100 map01110 map01130 map04152 map04976

Pfam Domains

Protein Families

HMG-CoA_red

Protein Analysis

563

Amino Acids

59.67

Weight (kDa)

6.62

Isoelectric Point (pI)

39.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG-CoA_red	PF00368	174 - 550	8e-147	Hydroxymethylglutaryl-coenzyme A reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76490
fragaria_vesca	FvH4_1g09980 FvH4_5g26180
malus_domestica	MD02G1108500.v1.1 MD02G1108700.v1.1 MD04G1238500.v1.1 MD08G1025200.v1.1 MD15G1227900.v1.1
prunus_persica	Prupe.7G187000_v2.0.a1 Prupe.7G187500_v2.0.a1
pyrus_communis	pycom02g08710 pycom02g08720 pycom15g20270
rosa_chinensis	RchiOBHm_Chr2g0096771 RchiOBHm_Chr7g0216871
rosa_laevigata	RLG00000002531 RLG00000016668
rosa_multiflora	Rmu_sc0000854.1_g000004 Rmu_sc0004291.1_g000010 Rmu_sc0030893.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145340 Rroxscaffold_3G00242800
rosa_rugosa	Rorug02G0060600 Rorug07G0164000.1
rosa_samantha	Rh2AG107800 Rh2BG110200 Rh2CG112100 Rh2DG111500 Rh7AG303400 Rh7BG295400 Rh7CG322100
rosa_wichuraiana	Rw2G008350 Rw7G025820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	347, 1412
AatII	GACGTC	1 cut(s)	345
Acc16I	TGCGCA	1 cut(s)	1293
Acc36I	ACCTGC	2 cut(s)	882, 1503
AccB1I	GGYRCC	1 cut(s)	697
AccII	CGCG	4 cut(s)	171, 534, 1316, 1565
AclI	AACGTT	1 cut(s)	1038
AclWI	GGATC	3 cut(s)	172, 640, 1334
AcoI	YGGCCR	4 cut(s)	729, 1266, 1333, 1551
AcuI	CTGAAG	2 cut(s)	917, 1223
AcyI	GRCGYC	2 cut(s)	342, 1029
AfaI	GTAC	6 cut(s)	482, 638, 692, 736, 971, 1200
AfiI	CCNNNNNNNGG	9 cut(s)	517, 546, 580, 584, 717, 784, 822, 1072, 1342
AflII	CTTAAG	1 cut(s)	1241
AgeI	ACCGGT	2 cut(s)	162, 554
AgsI	TTSAA	4 cut(s)	907, 944, 1288, 1536
AhdI	GACNNNNNGTC	1 cut(s)	1622
AjiI	CACGTC	1 cut(s)	202
AjnI	CCWGG	1 cut(s)	329
AjuI	GAANNNNNNNTTGG	2 cut(s)	104, 136
AloI	GAACNNNNNNTCC	2 cut(s)	1004, 1036
Alw21I	GWGCWC	6 cut(s)	222, 814, 1233, 1276, 1364, 1578
Alw26I	GTCTC	5 cut(s)	498, 1024, 1420, 1515, 1669
AlwI	GGATC	3 cut(s)	172, 640, 1334
Ama87I	CYCGRG	2 cut(s)	488, 807
ApaI	GGGCCC	1 cut(s)	778
ArsI	GACNNNNNNTTYG	2 cut(s)	544, 576
AsiGI	ACCGGT	2 cut(s)	162, 554
AspLEI	GCGC	5 cut(s)	88, 171, 317, 534, 1294
AspS9I	GGNCC	7 cut(s)	326, 538, 665, 774, 775, 871, 1405
AsuC2I	CCSGG	2 cut(s)	650, 1530
AsuHPI	GGTGA	6 cut(s)	100, 118, 1138, 1201, 1366, 1414
AvaI	CYCGRG	2 cut(s)	488, 807
AvaII	GGWCC	2 cut(s)	871, 1405
BaeGI	GKGCMC	1 cut(s)	778
BalI	TGGCCA	2 cut(s)	731, 1553
BanI	GGYRCC	1 cut(s)	697
BanII	GRGCYC	6 cut(s)	222, 778, 814, 1233, 1364, 1571
BarI	GAAGNNNNNNTAC	2 cut(s)	953, 985
BbsI	GAAGAC	1 cut(s)	1211
Bbv12I	GWGCWC	6 cut(s)	222, 814, 1233, 1276, 1364, 1578
BccI	CCATC	5 cut(s)	257, 458, 563, 764, 1375
BceAI	ACGGC	5 cut(s)	125, 340, 545, 881, 1414
BcgI	CGANNNNNNTGC	4 cut(s)	265, 299, 1291, 1325
BciT130I	CCWGG	1 cut(s)	331
BclI	TGATCA	1 cut(s)	1173
BcnI	CCSGG	2 cut(s)	650, 1530
BcoDI	GTCTC	5 cut(s)	498, 1024, 1420, 1515, 1669
BfaI	CTAG	4 cut(s)	1043, 1542, 1622, 1653
BfmI	CTRYAG	2 cut(s)	33, 542
BfoI	RGCGCY	1 cut(s)	318
BfrI	CTTAAG	1 cut(s)	1241
BfuAI	ACCTGC	2 cut(s)	882, 1503
BglII	AGATCT	1 cut(s)	1649
BlpI	GCTNAGC	1 cut(s)	832
Bme1390I	CCNGG	3 cut(s)	331, 650, 1530
Bme18I	GGWCC	2 cut(s)	871, 1405
BmeRI	GACNNNNNGTC	1 cut(s)	1622
BmeT110I	CYCGRG	2 cut(s)	488, 807
BmgBI	CACGTC	1 cut(s)	202
BmgT120I	GGNCC	7 cut(s)	326, 538, 665, 774, 775, 871, 1405
BmiI	GGNNCC	6 cut(s)	328, 666, 699, 775, 776, 1462
BmrFI	CCNGG	3 cut(s)	331, 650, 1530
BmrI	ACTGGG	2 cut(s)	617, 1677
BmsI	GCATC	7 cut(s)	73, 615, 737, 988, 1095, 1380, 1481
BmuI	ACTGGG	2 cut(s)	617, 1677
BpiI	GAAGAC	1 cut(s)	1211
BpmI	CTGGAG	2 cut(s)	295, 1014
Bpu1102I	GCTNAGC	1 cut(s)	832
BpuEI	CTTGAG	3 cut(s)	56, 776, 839
BpuMI	CCSGG	2 cut(s)	650, 1530
BsaAI	YACGTR	1 cut(s)	640
BsaBI	GATNNNNATC	1 cut(s)	1322
BsaHI	GRCGYC	2 cut(s)	342, 1029
BsaI	GGTCTC	1 cut(s)	1024
BsaJI	CCNNGG	6 cut(s)	262, 322, 329, 499, 711, 1221
BsaWI	WCCGGW	3 cut(s)	162, 554, 822
BsaXI	ACNNNNNCTCC	6 cut(s)	796, 826, 1004, 1034, 1088, 1118
Bsc4I	CCNNNNNNNGG	9 cut(s)	517, 546, 580, 584, 717, 784, 822, 1072, 1342
Bse118I	RCCGGY	9 cut(s)	12, 38, 162, 418, 554, 628, 1117, 1268, 1331
Bse1I	ACTGG	6 cut(s)	289, 612, 732, 997, 1258, 1683
Bse3DI	GCAATG	1 cut(s)	620
Bse8I	GATNNNNATC	1 cut(s)	1322
BseBI	CCWGG	1 cut(s)	331
BseDI	CCNNGG	6 cut(s)	262, 322, 329, 499, 711, 1221
BseGI	GGATG	5 cut(s)	574, 752, 756, 1014, 1666
BseJI	GATNNNNATC	1 cut(s)	1322
BseLI	CCNNNNNNNGG	9 cut(s)	517, 546, 580, 584, 717, 784, 822, 1072, 1342
BseMI	GCAATG	1 cut(s)	620
BseMII	CTCAG	3 cut(s)	846, 1361, 1584
BseNI	ACTGG	6 cut(s)	289, 612, 732, 997, 1258, 1683
BseRI	GAGGAG	5 cut(s)	146, 251, 379, 701, 1470
BseSI	GKGCMC	1 cut(s)	778
BseYI	CCCAGC	2 cut(s)	1464, 1498
BsgI	GTGCAG	2 cut(s)	182, 662
Bsh1236I	CGCG	4 cut(s)	171, 534, 1316, 1565
Bsh1285I	CGRYCG	1 cut(s)	555
BshNI	GGYRCC	1 cut(s)	697
BshTI	ACCGGT	2 cut(s)	162, 554
BsiEI	CGRYCG	1 cut(s)	555
BsiHKAI	GWGCWC	6 cut(s)	222, 814, 1233, 1276, 1364, 1578
BsiHKCI	CYCGRG	2 cut(s)	488, 807
BslFI	GGGAC	1 cut(s)	1629
BslI	CCNNNNNNNGG	9 cut(s)	517, 546, 580, 584, 717, 784, 822, 1072, 1342
BsmAI	GTCTC	5 cut(s)	498, 1024, 1420, 1515, 1669
BsmFI	GGGAC	1 cut(s)	1629
Bso31I	GGTCTC	1 cut(s)	1024
BsoBI	CYCGRG	2 cut(s)	488, 807
Bsp120I	GGGCCC	1 cut(s)	774
Bsp1286I	GDGCHC	8 cut(s)	222, 778, 814, 1233, 1276, 1364, 1571, 1578
Bsp1407I	TGTACA	1 cut(s)	969
Bsp143I	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
Bsp1720I	GCTNAGC	1 cut(s)	832
BspCNI	CTCAG	3 cut(s)	845, 1360, 1583
BspFNI	CGCG	4 cut(s)	171, 534, 1316, 1565
BspLI	GGNNCC	6 cut(s)	328, 666, 699, 775, 776, 1462
BspMAI	CTGCAG	2 cut(s)	37, 546
BspMI	ACCTGC	2 cut(s)	882, 1503
BspPI	GGATC	3 cut(s)	172, 640, 1334
BspT107I	GGYRCC	1 cut(s)	697
BspTI	CTTAAG	1 cut(s)	1241
BspTNI	GGTCTC	1 cut(s)	1024
BsrDI	GCAATG	1 cut(s)	620
BsrFI	RCCGGY	9 cut(s)	12, 38, 162, 418, 554, 628, 1117, 1268, 1331
BsrGI	TGTACA	1 cut(s)	969
BsrI	ACTGG	6 cut(s)	289, 612, 732, 997, 1258, 1683
BssAI	RCCGGY	9 cut(s)	12, 38, 162, 418, 554, 628, 1117, 1268, 1331
BssECI	CCNNGG	6 cut(s)	262, 322, 329, 499, 711, 1221
BssMI	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
BssNI	GRCGYC	2 cut(s)	342, 1029
BssT1I	CCWWGG	1 cut(s)	1221
Bst2UI	CCWGG	1 cut(s)	331
Bst4CI	ACNGT	3 cut(s)	697, 800, 1450
Bst6I	CTCTTC	4 cut(s)	125, 137, 236, 1176
BstACI	GRCGYC	2 cut(s)	342, 1029
BstAFI	CTTAAG	1 cut(s)	1241
BstAUI	TGTACA	1 cut(s)	969
BstBAI	YACGTR	1 cut(s)	640
BstDEI	CTNAG	3 cut(s)	832, 1347, 1570
BstDSI	CCRYGG	2 cut(s)	322, 711
BstF5I	GGATG	5 cut(s)	574, 752, 756, 1014, 1666
BstFNI	CGCG	4 cut(s)	171, 534, 1316, 1565
BstH2I	RGCGCY	1 cut(s)	318
BstHHI	GCGC	5 cut(s)	88, 171, 317, 534, 1294
BstKTI	GATC	8 cut(s)	180, 648, 678, 914, 1176, 1320, 1342, 1652
BstMAI	GTCTC	5 cut(s)	498, 1024, 1420, 1515, 1669
BstMBI	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
BstMCI	CGRYCG	1 cut(s)	555
BstNI	CCWGG	1 cut(s)	331
BstNSI	RCATGY	1 cut(s)	1240
BstSCI	CCNGG	3 cut(s)	329, 648, 1528
BstSFI	CTRYAG	2 cut(s)	33, 542
BstSLI	GKGCMC	1 cut(s)	778
BstUI	CGCG	4 cut(s)	171, 534, 1316, 1565
BstV2I	GAAGAC	1 cut(s)	1211
BstX2I	RGATCY	4 cut(s)	177, 645, 1339, 1649
BstXI	CCANNNNNNTGG	1 cut(s)	1441
BstYI	RGATCY	4 cut(s)	177, 645, 1339, 1649
BtgI	CCRYGG	2 cut(s)	322, 711
BtrI	CACGTC	1 cut(s)	202
BtsCI	GGATG	5 cut(s)	574, 752, 756, 1014, 1666
BtsI	GCAGTG	2 cut(s)	1176, 1366
BtsIMutI	CAGTG	5 cut(s)	990, 1161, 1176, 1366, 1578
BveI	ACCTGC	2 cut(s)	882, 1503
CfoI	GCGC	5 cut(s)	88, 171, 317, 534, 1294
Cfr10I	RCCGGY	9 cut(s)	12, 38, 162, 418, 554, 628, 1117, 1268, 1331
Cfr13I	GGNCC	7 cut(s)	326, 538, 665, 774, 775, 871, 1405
CseI	GACGC	2 cut(s)	834, 1018
Csp6I	GTAC	6 cut(s)	481, 637, 691, 735, 970, 1199
CspAI	ACCGGT	2 cut(s)	162, 554
CviAII	CATG	9 cut(s)	763, 973, 1015, 1072, 1237, 1378, 1412, 1426, 1636
CviQI	GTAC	6 cut(s)	481, 637, 691, 735, 970, 1199
DdeI	CTNAG	3 cut(s)	832, 1347, 1570
DpnI	GATC	8 cut(s)	179, 647, 677, 913, 1175, 1319, 1341, 1651
DpnII	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
DrdI	GACNNNNNNGTC	2 cut(s)	347, 1412
DriI	GACNNNNNGTC	1 cut(s)	1622
DseDI	GACNNNNNNGTC	2 cut(s)	347, 1412
EaeI	YGGCCR	4 cut(s)	729, 1266, 1333, 1551
Eam1104I	CTCTTC	4 cut(s)	125, 137, 236, 1176
Eam1105I	GACNNNNNGTC	1 cut(s)	1622
EarI	CTCTTC	4 cut(s)	125, 137, 236, 1176
EciI	GGCGGA	2 cut(s)	382, 391
Ecl136II	GAGCTC	4 cut(s)	220, 812, 1231, 1362
Eco130I	CCWWGG	1 cut(s)	1221
Eco147I	AGGCCT	1 cut(s)	77
Eco24I	GRGCYC	6 cut(s)	222, 778, 814, 1233, 1364, 1571
Eco31I	GGTCTC	1 cut(s)	1024
Eco47I	GGWCC	2 cut(s)	871, 1405
Eco53kI	GAGCTC	4 cut(s)	220, 812, 1231, 1362
Eco57I	CTGAAG	2 cut(s)	917, 1223
Eco88I	CYCGRG	2 cut(s)	488, 807
EcoICRI	GAGCTC	4 cut(s)	220, 812, 1231, 1362
EcoO109I	RGGNCCY	4 cut(s)	538, 774, 871, 1405
EcoRII	CCWGG	1 cut(s)	329
EcoT14I	CCWWGG	1 cut(s)	1221
EcoT38I	GRGCYC	6 cut(s)	222, 778, 814, 1233, 1364, 1571
ErhI	CCWWGG	1 cut(s)	1221
FaeI	CATG	9 cut(s)	766, 976, 1018, 1075, 1240, 1381, 1415, 1429, 1639
FaqI	GGGAC	1 cut(s)	1629
FatI	CATG	9 cut(s)	762, 972, 1014, 1071, 1236, 1377, 1411, 1425, 1635
FauI	CCCGC	5 cut(s)	585, 764, 785, 1558, 1606
FbaI	TGATCA	1 cut(s)	1173
FokI	GGATG	5 cut(s)	581, 743, 759, 1021, 1673
FriOI	GRGCYC	6 cut(s)	222, 778, 814, 1233, 1364, 1571
FspAI	RTGCGCAY	1 cut(s)	1293
FspBI	CTAG	4 cut(s)	1043, 1542, 1622, 1653
FspI	TGCGCA	1 cut(s)	1293
GlaI	GCGC	5 cut(s)	87, 170, 316, 533, 1293
GsaI	CCCAGC	2 cut(s)	1468, 1502
GsuI	CTGGAG	2 cut(s)	295, 1014
HaeII	RGCGCY	1 cut(s)	318
HgaI	GACGC	2 cut(s)	834, 1018
HhaI	GCGC	5 cut(s)	88, 171, 317, 534, 1294
Hin1I	GRCGYC	2 cut(s)	342, 1029
Hin1II	CATG	9 cut(s)	766, 976, 1018, 1075, 1240, 1381, 1415, 1429, 1639
Hin6I	GCGC	5 cut(s)	86, 169, 315, 532, 1292
HinP1I	GCGC	5 cut(s)	86, 169, 315, 532, 1292
HindIII	AAGCTT	1 cut(s)	929
HinfI	GANTC	4 cut(s)	413, 491, 940, 1523
HphI	GGTGA	6 cut(s)	100, 118, 1138, 1201, 1366, 1414
Hpy166II	GTNNAC	4 cut(s)	495, 693, 970, 981
Hpy188I	TCNGA	8 cut(s)	58, 82, 217, 503, 850, 936, 1108, 1350
Hpy188III	TCNNGA	5 cut(s)	488, 1043, 1092, 1542, 1627
Hpy8I	GTNNAC	4 cut(s)	495, 693, 970, 981
Hpy99I	CGWCG	7 cut(s)	146, 206, 347, 464, 683, 902, 1400
HpyAV	CCTTC	8 cut(s)	304, 486, 578, 1133, 1189, 1418, 1440, 1501
HpyCH4III	ACNGT	3 cut(s)	697, 800, 1450
HpyCH4IV	ACGT	4 cut(s)	201, 342, 639, 1038
HpyF3I	CTNAG	3 cut(s)	832, 1347, 1570
HpySE526I	ACGT	4 cut(s)	201, 342, 639, 1038
Hsp92I	GRCGYC	2 cut(s)	342, 1029
Hsp92II	CATG	9 cut(s)	766, 976, 1018, 1075, 1240, 1381, 1415, 1429, 1639
HspAI	GCGC	5 cut(s)	86, 169, 315, 532, 1292
KroI	GCCGGC	3 cut(s)	12, 38, 418
KroNI	GCCGGC	3 cut(s)	14, 40, 420
Ksp22I	TGATCA	1 cut(s)	1173
Kzo9I	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
LmnI	GCTCC	4 cut(s)	191, 817, 1109, 1228
LweI	GCATC	7 cut(s)	73, 615, 737, 988, 1095, 1380, 1481
MaeI	CTAG	4 cut(s)	1043, 1542, 1622, 1653
MaeII	ACGT	4 cut(s)	201, 342, 639, 1038
MaeIII	GTNAC	2 cut(s)	205, 1420
MalI	GATC	8 cut(s)	179, 647, 677, 913, 1175, 1319, 1341, 1651
MboI	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
MfeI	CAATTG	1 cut(s)	1605
MflI	RGATCY	4 cut(s)	177, 645, 1339, 1649
MhlI	GDGCHC	8 cut(s)	222, 778, 814, 1233, 1276, 1364, 1571, 1578
MlsI	TGGCCA	2 cut(s)	731, 1553
MluCI	AATT	4 cut(s)	370, 1129, 1547, 1605
MluNI	TGGCCA	2 cut(s)	731, 1553
MlyI	GAGTC	3 cut(s)	422, 500, 1517
MmeI	TCCRAC	3 cut(s)	81, 1131, 1432
Mox20I	TGGCCA	2 cut(s)	731, 1553
MreI	CGCCGGCG	2 cut(s)	12, 418
MroNI	GCCGGC	3 cut(s)	12, 38, 418
MscI	TGGCCA	2 cut(s)	731, 1553
MseI	TTAA	4 cut(s)	51, 374, 1242, 1687
MslI	CAYNNNNRTG	4 cut(s)	761, 1376, 1379, 1439
Msp20I	TGGCCA	2 cut(s)	731, 1553
MspA1I	CMGCKG	6 cut(s)	67, 196, 547, 580, 670, 1613
MspCI	CTTAAG	1 cut(s)	1241
MspR9I	CCNGG	3 cut(s)	331, 650, 1530
MunI	CAATTG	1 cut(s)	1605
MvaI	CCWGG	1 cut(s)	331
MvnI	CGCG	4 cut(s)	171, 534, 1316, 1565
NaeI	GCCGGC	3 cut(s)	14, 40, 420
NciI	CCSGG	2 cut(s)	650, 1530
NdeII	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
NgoMIV	GCCGGC	3 cut(s)	12, 38, 418
NlaIII	CATG	9 cut(s)	766, 976, 1018, 1075, 1240, 1381, 1415, 1429, 1639
NlaIV	GGNNCC	6 cut(s)	328, 666, 699, 775, 776, 1462
NmeAIII	GCCGAG	1 cut(s)	243
NmuCI	GTSAC	2 cut(s)	205, 1420
NsbI	TGCGCA	1 cut(s)	1293
NspI	RCATGY	1 cut(s)	1240
PaeR7I	CTCGAG	1 cut(s)	488
PceI	AGGCCT	1 cut(s)	77
PcsI	WCGNNNNNNNCGW	1 cut(s)	342
PdiI	GCCGGC	3 cut(s)	14, 40, 420
PfeI	GAWTC	1 cut(s)	940
PflFI	GACNNNGTC	1 cut(s)	284
PinAI	ACCGGT	2 cut(s)	162, 554
PleI	GAGTC	3 cut(s)	421, 499, 1517
PpsI	GAGTC	3 cut(s)	421, 499, 1517
Ppu21I	YACGTR	1 cut(s)	640
PpuMI	RGGWCCY	2 cut(s)	871, 1405
Psp124BI	GAGCTC	4 cut(s)	222, 814, 1233, 1364
Psp1406I	AACGTT	1 cut(s)	1038
Psp5II	RGGWCCY	2 cut(s)	871, 1405
Psp6I	CCWGG	1 cut(s)	329
PspFI	CCCAGC	2 cut(s)	1464, 1498
PspGI	CCWGG	1 cut(s)	329
PspN4I	GGNNCC	6 cut(s)	328, 666, 699, 775, 776, 1462
PspOMI	GGGCCC	1 cut(s)	774
PspPI	GGNCC	7 cut(s)	326, 538, 665, 774, 775, 871, 1405
PspPPI	RGGWCCY	2 cut(s)	871, 1405
PstI	CTGCAG	2 cut(s)	37, 546
PsuI	RGATCY	4 cut(s)	177, 645, 1339, 1649
PsyI	GACNNNGTC	1 cut(s)	284
PvuII	CAGCTG	1 cut(s)	67
RsaI	GTAC	6 cut(s)	482, 638, 692, 736, 971, 1200
RsaNI	GTAC	6 cut(s)	481, 637, 691, 735, 970, 1199
RseI	CAYNNNNRTG	4 cut(s)	761, 1376, 1379, 1439
SacI	GAGCTC	4 cut(s)	222, 814, 1233, 1364
SaqAI	TTAA	4 cut(s)	51, 374, 1242, 1687
Sau3AI	GATC	8 cut(s)	177, 645, 675, 911, 1173, 1317, 1339, 1649
Sau96I	GGNCC	7 cut(s)	326, 538, 665, 774, 775, 871, 1405
SchI	GAGTC	3 cut(s)	422, 500, 1517
ScrFI	CCNGG	3 cut(s)	331, 650, 1530
SduI	GDGCHC	8 cut(s)	222, 778, 814, 1233, 1276, 1364, 1571, 1578
SfaNI	GCATC	7 cut(s)	73, 615, 737, 988, 1095, 1380, 1481
SfcI	CTRYAG	2 cut(s)	33, 542
Sfr274I	CTCGAG	1 cut(s)	488
SgrAI	CRCCGGYG	2 cut(s)	12, 418
SinI	GGWCC	2 cut(s)	871, 1405
SlaI	CTCGAG	1 cut(s)	488
SmiMI	CAYNNNNRTG	4 cut(s)	761, 1376, 1379, 1439
SmlI	CTYRAG	5 cut(s)	71, 488, 791, 854, 1241
SmoI	CTYRAG	5 cut(s)	71, 488, 791, 854, 1241
Sse9I	AATT	4 cut(s)	370, 1129, 1547, 1605
SseBI	AGGCCT	1 cut(s)	77
SspMI	CTAG	4 cut(s)	1043, 1542, 1622, 1653
SstI	GAGCTC	4 cut(s)	222, 814, 1233, 1364
StuI	AGGCCT	1 cut(s)	77
StyD4I	CCNGG	3 cut(s)	329, 648, 1528
StyI	CCWWGG	1 cut(s)	1221
TaaI	ACNGT	3 cut(s)	697, 800, 1450
TaiI	ACGT	4 cut(s)	204, 345, 642, 1041
TaqI	TCGA	5 cut(s)	462, 489, 591, 678, 950
TasI	AATT	4 cut(s)	370, 1129, 1547, 1605
TatI	WGTACW	2 cut(s)	690, 969
TfiI	GAWTC	1 cut(s)	940
Tru1I	TTAA	4 cut(s)	51, 374, 1242, 1687
Tru9I	TTAA	4 cut(s)	51, 374, 1242, 1687
TscAI	CASTG	5 cut(s)	997, 1161, 1176, 1373, 1578
TseFI	GTSAC	2 cut(s)	205, 1420
Tsp45I	GTSAC	2 cut(s)	205, 1420
TspDTI	ATGAA	3 cut(s)	1025, 1400, 1652
TspGWI	ACGGA	2 cut(s)	728, 1408
TspRI	CASTG	5 cut(s)	997, 1161, 1176, 1373, 1578
Tth111I	GACNNNGTC	1 cut(s)	284
Vha464I	CTTAAG	1 cut(s)	1241
VpaK11BI	GGWCC	2 cut(s)	871, 1405
XbaI	TCTAGA	2 cut(s)	1042, 1541
XceI	RCATGY	1 cut(s)	1240
XhoI	CTCGAG	1 cut(s)	488
XspI	CTAG	4 cut(s)	1043, 1542, 1622, 1653
ZraI	GACGTC	1 cut(s)	343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.