Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003985

Physical Location & Seq

Genomic Coordinates Reverse (-)

18530226 .. 18534489

Gene Structure 4264 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1227900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGGCAGTTCGCCGGCGACCCTCAACCGCCTCTGCCGCCGGCGAACCCATTAAGTCCGACAAGCAGCTGCTTAAGGCCTCCGACGCCCTTCCCCTCCCGCTCTACCTCACCAATGGCCTCTTCTTCACGCTCTTCTTCACCGTCGCCTACTTCCTCCTCACCCGCTGGCGAGAGAAGATCCGCAGCTCCACGCCACTTCACGTCGTCACGCTCTCTGAGCTCGCCGCCCTCTTCTCCCTCTTCGCCTCCGTTATCTACCTACTCGGCTTCTTCGGCATTGACTTCGTCCAGTCCTTCGTCTCCCCCAGCTCCCGCGCCGCCCACGACCCCTGGAAAGACGACGTTGTAGTCCAGCGAAAAAAATCCGTAAATGCCAGCGCACTCCTTTCCTCCACCTCCGCCGCTACAACCACCGCAACCGCCATCGACGATAATTCGCCGGCGTTGTCGACCGATGACGAGGAAATTATCACGTCTGTCGTCGTCGGAACCACGCCGTCATACTCTCTCGAGTCCACTCTCGGGGACTGCCGGCGAGCTGCTGCGATACGGCGAGAGGCTCTGCAGCGGACAACCGGTAGTTCGCTGGTGGGGCTCCCGCTGGAAGGCTTCGACTACGAGGCGATACTGGGGCAGTGCTGCGAGATGCCGGTCGGGTACGTCCAGATCCCGGTGGGAGTGGCGGGGCCGCTGCTGATCGACGACGAGGAGTACACGGTGCCAATGGCGACGACGGAGGGATGCTTGGTTGCCAGCACCAATAGGGGCTGCAAGGCCATCCACATGTCAGGCGGGGCCGGTAGCGTGGTTCTCAAGGACGGGATGACCCGAGCTCCCGTTGTCCGGTTCAGCTCAGCCGCCAGAGCTGCCGAACTCAAGTTCTTCGTCGAGGACCCGCTCAACTTCGATTCCCTCGCTGTCGTGTTCAACAGATCTAGTAGATTTGCAAAGCTTCAGAGGATTCAATGCTCGATGGCAGGGAAGAATTTGTACATGAGGTTTACTTGCAGCACTGGAGATGCAATGGGGATGAACATGGTCTCCAAAGGCGTCCAAAACGTTCTAGATTTCCTCCAAATCGACTTCCCTGACATGGATGTTATTGGCATCTCTGGAAACTTTTGCTCCGACAAGAAACCGGCCGCGGTGAATTGGATAGAAGGGAGAGGCAAGTCAGTAGTGTGTGAAGCAGTGATCAAGGAAGAGGTGGTGAGGAAGGTACTGAAGACCAATGTGGCTGCCTTGGTGGAGCTCAACATGCTCAAAAACCTAACTGGTTCTGCCATGGCTGGTGCTCTCGGCGGGTTCAATGCCCACGCCAGCAACATTGTTGCTGCGATCTACATCGCCACAGGTCAGGATCCGGCTCAGAATGTAGAGAGCTCTCACTGCATCACCATGATGGAAGCCATAAACGACGGCAAGGACCTTCATGTCTCAGTCACCATGCCTTCCATTGAGGTGGGAACAGTTGGAGGAGGAACCCAGCTTGCATCCCAAGCAGCCTGCTTGAACCTGCTGGGTGTAAAGGGTGCAAGCAGAGACTCGCCCGGTTCAAACTCTAGGAAATTGGCCACCATTGTAGCCGGTTCAGTGCTCGCCGGAGAGCTCTCGCTGATGTCAGCAATTGCAGCTGGACAGCTGGTCAATAGCCACATGAAATACAACAGATCTAGCAAGGATGTCTCAAAACTTGCCGGCCCCCGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004420 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006720 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016125 GO:0016126 GO:0016491 GO:0016614 GO:0016616 GO:0031090 GO:0031903 GO:0031984 GO:0042175 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098588 GO:0098805 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 ko04152 ko04976 map00900 map01100 map01110 map01130 map04152 map04976

Pfam Domains

Protein Families

HMG-CoA_red

Protein Analysis

570

Amino Acids

60.36

Weight (kDa)

7.51

Isoelectric Point (pI)

37.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG-CoA_red	PF00368	181 - 557	1.7e-145	Hydroxymethylglutaryl-coenzyme A reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76490
fragaria_vesca	FvH4_1g09980 FvH4_5g26180
malus_domestica	MD02G1108500.v1.1 MD02G1108700.v1.1 MD04G1238500.v1.1 MD08G1025200.v1.1 MD15G1227900.v1.1
prunus_persica	Prupe.7G187000_v2.0.a1 Prupe.7G187500_v2.0.a1
pyrus_communis	pycom02g08710 pycom02g08720 pycom15g20270
rosa_chinensis	RchiOBHm_Chr2g0096771 RchiOBHm_Chr7g0216871
rosa_laevigata	RLG00000002531 RLG00000016668
rosa_multiflora	Rmu_sc0000854.1_g000004 Rmu_sc0004291.1_g000010 Rmu_sc0030893.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145340 Rroxscaffold_3G00242800
rosa_rugosa	Rorug02G0060600 Rorug07G0164000.1
rosa_samantha	Rh2AG107800 Rh2BG110200 Rh2CG112100 Rh2DG111500 Rh7AG303400 Rh7BG295400 Rh7CG322100
rosa_wichuraiana	Rw2G008350 Rw7G025820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	347, 1433
Acc36I	ACCTGC	1 cut(s)	1524
AccB1I	GGYRCC	1 cut(s)	718
AccBSI	CCGCTC	2 cut(s)	100, 898
AccI	GTMKAC	1 cut(s)	449
AccII	CGCG	2 cut(s)	315, 1145
AclI	AACGTT	1 cut(s)	1059
AclWI	GGATC	4 cut(s)	172, 661, 1355, 1368
AcoI	YGGCCR	2 cut(s)	1140, 1572
AcsI	RAATTY	1 cut(s)	985
AcuI	CTGAAG	2 cut(s)	938, 1244
AcyI	GRCGYC	2 cut(s)	84, 1050
AfaI	GTAC	4 cut(s)	659, 713, 992, 1221
AfiI	CCNNNNNNNGG	5 cut(s)	522, 605, 670, 843, 1093
AflII	CTTAAG	1 cut(s)	71
AflIII	ACRYGT	1 cut(s)	783
AgeI	ACCGGT	1 cut(s)	575
AgsI	TTSAA	5 cut(s)	928, 965, 1309, 1513, 1557
AhdI	GACNNNNNGTC	1 cut(s)	1643
AjiI	CACGTC	2 cut(s)	202, 474
AjnI	CCWGG	1 cut(s)	329
AjuI	GAANNNNNNNTTGG	2 cut(s)	104, 136
AloI	GAACNNNNNNTCC	2 cut(s)	1025, 1057
Alw21I	GWGCWC	7 cut(s)	222, 835, 1254, 1297, 1385, 1599, 1611
Alw26I	GTCTC	5 cut(s)	304, 1045, 1441, 1536, 1690
AlwI	GGATC	4 cut(s)	172, 661, 1355, 1368
Ama87I	CYCGRG	3 cut(s)	509, 521, 828
AoxI	GGCC	8 cut(s)	75, 115, 686, 774, 795, 1140, 1572, 1699
ApoI	RAATTY	1 cut(s)	985
AsiGI	ACCGGT	1 cut(s)	575
AspLEI	GCGC	2 cut(s)	317, 380
AspS9I	GGNCC	5 cut(s)	686, 795, 892, 1426, 1700
AsuC2I	CCSGG	2 cut(s)	671, 1551
AsuHPI	GGTGA	7 cut(s)	100, 130, 151, 1159, 1222, 1387, 1435
AvaI	CYCGRG	3 cut(s)	509, 521, 828
AvaII	GGWCC	2 cut(s)	892, 1426
BalI	TGGCCA	1 cut(s)	1574
BamHI	GGATCC	1 cut(s)	1360
BanI	GGYRCC	1 cut(s)	718
BanII	GRGCYC	6 cut(s)	222, 597, 835, 1254, 1385, 1611
BarI	GAAGNNNNNNTAC	2 cut(s)	974, 1006
BbsI	GAAGAC	1 cut(s)	1232
Bbv12I	GWGCWC	7 cut(s)	222, 835, 1254, 1297, 1385, 1599, 1611
BccI	CCATC	4 cut(s)	431, 785, 967, 1396
BceAI	ACGGC	3 cut(s)	481, 566, 1435
BcgI	CGANNNNNNTGC	2 cut(s)	265, 299
BciT130I	CCWGG	1 cut(s)	331
BclI	TGATCA	1 cut(s)	1194
BcnI	CCSGG	2 cut(s)	671, 1551
BcoDI	GTCTC	5 cut(s)	304, 1045, 1441, 1536, 1690
BfaI	CTAG	4 cut(s)	936, 1064, 1563, 1674
BfmI	CTRYAG	1 cut(s)	563
BfrI	CTTAAG	1 cut(s)	71
BfuAI	ACCTGC	1 cut(s)	1524
BglII	AGATCT	2 cut(s)	932, 1670
BlpI	GCTNAGC	1 cut(s)	853
Bme1390I	CCNGG	3 cut(s)	331, 671, 1551
Bme18I	GGWCC	2 cut(s)	892, 1426
BmeRI	GACNNNNNGTC	1 cut(s)	1643
BmeT110I	CYCGRG	3 cut(s)	509, 521, 828
BmgBI	CACGTC	2 cut(s)	202, 474
BmgT120I	GGNCC	5 cut(s)	686, 795, 892, 1426, 1700
BmiI	GGNNCC	9 cut(s)	490, 596, 687, 720, 796, 894, 1362, 1483, 1702
BmrFI	CCNGG	3 cut(s)	331, 671, 1551
BmrI	ACTGGG	1 cut(s)	638
BmsI	GCATC	6 cut(s)	636, 731, 1009, 1116, 1401, 1502
BmuI	ACTGGG	1 cut(s)	638
BpiI	GAAGAC	1 cut(s)	1232
BpmI	CTGGAG	1 cut(s)	1035
Bpu1102I	GCTNAGC	1 cut(s)	853
BpuEI	CTTGAG	2 cut(s)	797, 860
BpuMI	CCSGG	2 cut(s)	671, 1551
BsaBI	GATNNNNATC	1 cut(s)	1343
BsaHI	GRCGYC	2 cut(s)	84, 1050
BsaI	GGTCTC	1 cut(s)	1045
BsaJI	CCNNGG	4 cut(s)	329, 1143, 1242, 1284
BsaWI	WCCGGW	2 cut(s)	575, 843
BsaXI	ACNNNNNCTCC	6 cut(s)	817, 847, 1025, 1055, 1109, 1139
Bsc4I	CCNNNNNNNGG	5 cut(s)	522, 605, 670, 843, 1093
Bse1I	ACTGG	4 cut(s)	289, 633, 1018, 1279
Bse3DI	GCAATG	1 cut(s)	1029
Bse8I	GATNNNNATC	1 cut(s)	1343
BseBI	CCWGG	1 cut(s)	331
BseDI	CCNNGG	4 cut(s)	329, 1143, 1242, 1284
BseGI	GGATG	7 cut(s)	746, 777, 828, 1035, 1102, 1493, 1687
BseJI	GATNNNNATC	1 cut(s)	1343
BseLI	CCNNNNNNNGG	5 cut(s)	522, 605, 670, 843, 1093
BseMI	GCAATG	1 cut(s)	1029
BseMII	CTCAG	4 cut(s)	207, 867, 1382, 1452
BseNI	ACTGG	4 cut(s)	289, 633, 1018, 1279
BseRI	GAGGAG	3 cut(s)	146, 722, 1491
BseX3I	CGGCCG	1 cut(s)	1140
BseYI	CCCAGC	3 cut(s)	305, 1485, 1519
Bsh1236I	CGCG	2 cut(s)	315, 1145
Bsh1285I	CGRYCG	3 cut(s)	453, 654, 1143
BshFI	GGCC	8 cut(s)	77, 117, 688, 776, 797, 1142, 1574, 1701
BshNI	GGYRCC	1 cut(s)	718
BshTI	ACCGGT	1 cut(s)	575
BsiEI	CGRYCG	3 cut(s)	453, 654, 1143
BsiHKAI	GWGCWC	7 cut(s)	222, 835, 1254, 1297, 1385, 1599, 1611
BsiHKCI	CYCGRG	3 cut(s)	509, 521, 828
BslFI	GGGAC	1 cut(s)	539
BslI	CCNNNNNNNGG	5 cut(s)	522, 605, 670, 843, 1093
BsmAI	GTCTC	5 cut(s)	304, 1045, 1441, 1536, 1690
BsmBI	CGTCTC	1 cut(s)	304
BsmFI	GGGAC	1 cut(s)	539
BsnI	GGCC	8 cut(s)	77, 117, 688, 776, 797, 1142, 1574, 1701
Bso31I	GGTCTC	1 cut(s)	1045
BsoBI	CYCGRG	3 cut(s)	509, 521, 828
Bsp1286I	GDGCHC	8 cut(s)	222, 597, 835, 1254, 1297, 1385, 1599, 1611
Bsp1407I	TGTACA	1 cut(s)	990
Bsp143I	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
Bsp1720I	GCTNAGC	1 cut(s)	853
Bsp19I	CCATGG	1 cut(s)	1284
BspANI	GGCC	8 cut(s)	77, 117, 688, 776, 797, 1142, 1574, 1701
BspCNI	CTCAG	4 cut(s)	208, 866, 1381, 1451
BspFNI	CGCG	2 cut(s)	315, 1145
BspLI	GGNNCC	9 cut(s)	490, 596, 687, 720, 796, 894, 1362, 1483, 1702
BspMAI	CTGCAG	1 cut(s)	567
BspMI	ACCTGC	1 cut(s)	1524
BspPI	GGATC	4 cut(s)	172, 661, 1355, 1368
BspQI	GCTCTTC	1 cut(s)	137
BspT107I	GGYRCC	1 cut(s)	718
BspTI	CTTAAG	1 cut(s)	71
BspTNI	GGTCTC	1 cut(s)	1045
BsrBI	CCGCTC	2 cut(s)	100, 898
BsrDI	GCAATG	1 cut(s)	1029
BsrGI	TGTACA	1 cut(s)	990
BsrI	ACTGG	4 cut(s)	289, 633, 1018, 1279
BssECI	CCNNGG	4 cut(s)	329, 1143, 1242, 1284
BssMI	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
BssNI	GRCGYC	2 cut(s)	84, 1050
BssT1I	CCWWGG	2 cut(s)	1242, 1284
Bst2UI	CCWGG	1 cut(s)	331
Bst4CI	ACNGT	3 cut(s)	142, 718, 1471
Bst6I	CTCTTC	5 cut(s)	125, 137, 236, 245, 1197
BstACI	GRCGYC	2 cut(s)	84, 1050
BstAFI	CTTAAG	1 cut(s)	71
BstAUI	TGTACA	1 cut(s)	990
BstDEI	CTNAG	4 cut(s)	216, 853, 1368, 1438
BstDSI	CCRYGG	2 cut(s)	1143, 1284
BstF5I	GGATG	7 cut(s)	746, 777, 828, 1035, 1102, 1493, 1687
BstFNI	CGCG	2 cut(s)	315, 1145
BstHHI	GCGC	2 cut(s)	317, 380
BstKTI	GATC	8 cut(s)	180, 669, 699, 935, 1197, 1341, 1363, 1673
BstMAI	GTCTC	5 cut(s)	304, 1045, 1441, 1536, 1690
BstMBI	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
BstMCI	CGRYCG	3 cut(s)	453, 654, 1143
BstNI	CCWGG	1 cut(s)	331
BstNSI	RCATGY	2 cut(s)	787, 1261
BstSCI	CCNGG	3 cut(s)	329, 669, 1549
BstSFI	CTRYAG	1 cut(s)	563
BstUI	CGCG	2 cut(s)	315, 1145
BstV2I	GAAGAC	1 cut(s)	1232
BstX2I	RGATCY	5 cut(s)	177, 666, 932, 1360, 1670
BstXI	CCANNNNNNTGG	1 cut(s)	1462
BstYI	RGATCY	5 cut(s)	177, 666, 932, 1360, 1670
BstZI	CGGCCG	1 cut(s)	1140
BsuRI	GGCC	8 cut(s)	77, 117, 688, 776, 797, 1142, 1574, 1701
BtgI	CCRYGG	2 cut(s)	1143, 1284
BtgZI	GCGATG	1 cut(s)	1330
BtrI	CACGTC	2 cut(s)	202, 474
BtsCI	GGATG	7 cut(s)	746, 777, 828, 1035, 1102, 1493, 1687
BtsI	GCAGTG	3 cut(s)	641, 1197, 1387
BtsIMutI	CAGTG	5 cut(s)	641, 1011, 1197, 1387, 1599
BveI	ACCTGC	1 cut(s)	1524
CfoI	GCGC	2 cut(s)	317, 380
Cfr13I	GGNCC	5 cut(s)	686, 795, 892, 1426, 1700
Cfr42I	CCGCGG	1 cut(s)	1146
CseI	GACGC	2 cut(s)	92, 1039
Csp6I	GTAC	4 cut(s)	658, 712, 991, 1220
CspAI	ACCGGT	1 cut(s)	575
CviQI	GTAC	4 cut(s)	658, 712, 991, 1220
DdeI	CTNAG	4 cut(s)	216, 853, 1368, 1438
DpnI	GATC	8 cut(s)	179, 668, 698, 934, 1196, 1340, 1362, 1672
DpnII	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
DrdI	GACNNNNNNGTC	2 cut(s)	347, 1433
DriI	GACNNNNNGTC	1 cut(s)	1643
DseDI	GACNNNNNNGTC	2 cut(s)	347, 1433
EaeI	YGGCCR	2 cut(s)	1140, 1572
EagI	CGGCCG	1 cut(s)	1140
Eam1104I	CTCTTC	5 cut(s)	125, 137, 236, 245, 1197
Eam1105I	GACNNNNNGTC	1 cut(s)	1643
EarI	CTCTTC	5 cut(s)	125, 137, 236, 245, 1197
EciI	GGCGGA	1 cut(s)	388
Ecl136II	GAGCTC	5 cut(s)	220, 833, 1252, 1383, 1609
EclXI	CGGCCG	1 cut(s)	1140
Eco130I	CCWWGG	2 cut(s)	1242, 1284
Eco147I	AGGCCT	1 cut(s)	77
Eco24I	GRGCYC	6 cut(s)	222, 597, 835, 1254, 1385, 1611
Eco31I	GGTCTC	1 cut(s)	1045
Eco47I	GGWCC	2 cut(s)	892, 1426
Eco52I	CGGCCG	1 cut(s)	1140
Eco53kI	GAGCTC	5 cut(s)	220, 833, 1252, 1383, 1609
Eco57I	CTGAAG	2 cut(s)	938, 1244
Eco88I	CYCGRG	3 cut(s)	509, 521, 828
EcoICRI	GAGCTC	5 cut(s)	220, 833, 1252, 1383, 1609
EcoO109I	RGGNCCY	2 cut(s)	892, 1426
EcoRII	CCWGG	1 cut(s)	329
EcoT14I	CCWWGG	2 cut(s)	1242, 1284
EcoT38I	GRGCYC	6 cut(s)	222, 597, 835, 1254, 1385, 1611
ErhI	CCWWGG	2 cut(s)	1242, 1284
Esp3I	CGTCTC	1 cut(s)	304
FaqI	GGGAC	1 cut(s)	539
FauI	CCCGC	8 cut(s)	105, 170, 320, 606, 676, 785, 903, 1295
FbaI	TGATCA	1 cut(s)	1194
FblI	GTMKAC	1 cut(s)	449
FokI	GGATG	7 cut(s)	753, 764, 835, 1042, 1109, 1480, 1694
FriOI	GRGCYC	6 cut(s)	222, 597, 835, 1254, 1385, 1611
FspBI	CTAG	4 cut(s)	936, 1064, 1563, 1674
GlaI	GCGC	2 cut(s)	316, 379
GsaI	CCCAGC	3 cut(s)	309, 1489, 1523
GsuI	CTGGAG	1 cut(s)	1035
HaeIII	GGCC	8 cut(s)	77, 117, 688, 776, 797, 1142, 1574, 1701
HgaI	GACGC	2 cut(s)	92, 1039
HhaI	GCGC	2 cut(s)	317, 380
Hin1I	GRCGYC	2 cut(s)	84, 1050
Hin6I	GCGC	2 cut(s)	315, 378
HinP1I	GCGC	2 cut(s)	315, 378
HincII	GTYRAC	1 cut(s)	450
HindII	GTYRAC	1 cut(s)	450
HindIII	AAGCTT	1 cut(s)	950
HinfI	GANTC	4 cut(s)	512, 908, 961, 1544
HphI	GGTGA	7 cut(s)	100, 130, 151, 1159, 1222, 1387, 1435
Hpy166II	GTNNAC	4 cut(s)	450, 516, 714, 1002
Hpy188I	TCNGA	7 cut(s)	58, 82, 217, 488, 957, 1129, 1371
Hpy188III	TCNNGA	5 cut(s)	509, 664, 1064, 1113, 1358
Hpy8I	GTNNAC	4 cut(s)	450, 516, 714, 1002
HpyAV	CCTTC	7 cut(s)	98, 304, 599, 1154, 1210, 1439, 1461
HpyCH4III	ACNGT	3 cut(s)	142, 718, 1471
HpyCH4IV	ACGT	5 cut(s)	201, 342, 473, 660, 1059
HpyCH4V	TGCA	9 cut(s)	565, 771, 947, 1008, 1022, 1392, 1493, 1535, 1631
HpyF3I	CTNAG	4 cut(s)	216, 853, 1368, 1438
HpySE526I	ACGT	5 cut(s)	201, 342, 473, 660, 1059
Hsp92I	GRCGYC	2 cut(s)	84, 1050
HspAI	GCGC	2 cut(s)	315, 378
KroI	GCCGGC	5 cut(s)	12, 38, 439, 531, 1697
KroNI	GCCGGC	5 cut(s)	14, 40, 441, 533, 1699
Ksp22I	TGATCA	1 cut(s)	1194
KspI	CCGCGG	1 cut(s)	1146
Kzo9I	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
LguI	GCTCTTC	1 cut(s)	137
LmnI	GCTCC	6 cut(s)	191, 314, 600, 838, 1130, 1249
LweI	GCATC	6 cut(s)	636, 731, 1009, 1116, 1401, 1502
MaeI	CTAG	4 cut(s)	936, 1064, 1563, 1674
MaeII	ACGT	5 cut(s)	201, 342, 473, 660, 1059
MaeIII	GTNAC	2 cut(s)	205, 1441
MalI	GATC	8 cut(s)	179, 668, 698, 934, 1196, 1340, 1362, 1672
MbiI	CCGCTC	2 cut(s)	100, 898
MboI	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
MfeI	CAATTG	1 cut(s)	1626
MflI	RGATCY	5 cut(s)	177, 666, 932, 1360, 1670
MhlI	GDGCHC	8 cut(s)	222, 597, 835, 1254, 1297, 1385, 1599, 1611
MlsI	TGGCCA	1 cut(s)	1574
MluCI	AATT	6 cut(s)	433, 465, 985, 1150, 1568, 1626
MluNI	TGGCCA	1 cut(s)	1574
MlyI	GAGTC	2 cut(s)	521, 1538
MmeI	TCCRAC	5 cut(s)	81, 105, 466, 1152, 1453
Mox20I	TGGCCA	1 cut(s)	1574
MreI	CGCCGGCG	3 cut(s)	12, 38, 439
MroNI	GCCGGC	5 cut(s)	12, 38, 439, 531, 1697
MscI	TGGCCA	1 cut(s)	1574
MseI	TTAA	2 cut(s)	51, 72
MslI	CAYNNNNRTG	4 cut(s)	782, 1397, 1400, 1460
Msp20I	TGGCCA	1 cut(s)	1574
MspA1I	CMGCKG	8 cut(s)	67, 165, 568, 601, 691, 1145, 1634, 1642
MspCI	CTTAAG	1 cut(s)	71
MspR9I	CCNGG	3 cut(s)	331, 671, 1551
MunI	CAATTG	1 cut(s)	1626
MvaI	CCWGG	1 cut(s)	331
MvnI	CGCG	2 cut(s)	315, 1145
NaeI	GCCGGC	5 cut(s)	14, 40, 441, 533, 1699
NciI	CCSGG	2 cut(s)	671, 1551
NcoI	CCATGG	1 cut(s)	1284
NdeII	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
NgoMIV	GCCGGC	5 cut(s)	12, 38, 439, 531, 1697
NlaIV	GGNNCC	9 cut(s)	490, 596, 687, 720, 796, 894, 1362, 1483, 1702
NmeAIII	GCCGAG	2 cut(s)	243, 1278
NmuCI	GTSAC	2 cut(s)	205, 1441
NspI	RCATGY	2 cut(s)	787, 1261
PaeR7I	CTCGAG	1 cut(s)	509
PceI	AGGCCT	1 cut(s)	77
PciI	ACATGT	1 cut(s)	783
PciSI	GCTCTTC	1 cut(s)	137
PdiI	GCCGGC	5 cut(s)	14, 40, 441, 533, 1699
PfeI	GAWTC	2 cut(s)	908, 961
PflFI	GACNNNGTC	1 cut(s)	284
PinAI	ACCGGT	1 cut(s)	575
PleI	GAGTC	2 cut(s)	520, 1538
PpsI	GAGTC	2 cut(s)	520, 1538
PpuMI	RGGWCCY	2 cut(s)	892, 1426
PscI	ACATGT	1 cut(s)	783
Psp124BI	GAGCTC	5 cut(s)	222, 835, 1254, 1385, 1611
Psp1406I	AACGTT	1 cut(s)	1059
Psp5II	RGGWCCY	2 cut(s)	892, 1426
Psp6I	CCWGG	1 cut(s)	329
PspFI	CCCAGC	3 cut(s)	305, 1485, 1519
PspGI	CCWGG	1 cut(s)	329
PspN4I	GGNNCC	9 cut(s)	490, 596, 687, 720, 796, 894, 1362, 1483, 1702
PspPI	GGNCC	5 cut(s)	686, 795, 892, 1426, 1700
PspPPI	RGGWCCY	2 cut(s)	892, 1426
PstI	CTGCAG	1 cut(s)	567
PsuI	RGATCY	5 cut(s)	177, 666, 932, 1360, 1670
PsyI	GACNNNGTC	1 cut(s)	284
PvuII	CAGCTG	3 cut(s)	67, 1634, 1642
RsaI	GTAC	4 cut(s)	659, 713, 992, 1221
RsaNI	GTAC	4 cut(s)	658, 712, 991, 1220
RseI	CAYNNNNRTG	4 cut(s)	782, 1397, 1400, 1460
SacI	GAGCTC	5 cut(s)	222, 835, 1254, 1385, 1611
SacII	CCGCGG	1 cut(s)	1146
SalI	GTCGAC	1 cut(s)	448
SapI	GCTCTTC	1 cut(s)	137
SaqAI	TTAA	2 cut(s)	51, 72
Sau3AI	GATC	8 cut(s)	177, 666, 696, 932, 1194, 1338, 1360, 1670
Sau96I	GGNCC	5 cut(s)	686, 795, 892, 1426, 1700
SchI	GAGTC	2 cut(s)	521, 1538
ScrFI	CCNGG	3 cut(s)	331, 671, 1551
SduI	GDGCHC	8 cut(s)	222, 597, 835, 1254, 1297, 1385, 1599, 1611
SfaNI	GCATC	6 cut(s)	636, 731, 1009, 1116, 1401, 1502
SfcI	CTRYAG	1 cut(s)	563
Sfr274I	CTCGAG	1 cut(s)	509
Sfr303I	CCGCGG	1 cut(s)	1146
SgrAI	CRCCGGYG	3 cut(s)	12, 38, 439
SgrBI	CCGCGG	1 cut(s)	1146
SinI	GGWCC	2 cut(s)	892, 1426
SlaI	CTCGAG	1 cut(s)	509
SmiMI	CAYNNNNRTG	4 cut(s)	782, 1397, 1400, 1460
SmlI	CTYRAG	4 cut(s)	71, 509, 812, 875
SmoI	CTYRAG	4 cut(s)	71, 509, 812, 875
Sse9I	AATT	6 cut(s)	433, 465, 985, 1150, 1568, 1626
SseBI	AGGCCT	1 cut(s)	77
SspMI	CTAG	4 cut(s)	936, 1064, 1563, 1674
SstI	GAGCTC	5 cut(s)	222, 835, 1254, 1385, 1611
StuI	AGGCCT	1 cut(s)	77
StyD4I	CCNGG	3 cut(s)	329, 669, 1549
StyI	CCWWGG	2 cut(s)	1242, 1284
TaaI	ACNGT	3 cut(s)	142, 718, 1471
TaiI	ACGT	5 cut(s)	204, 345, 476, 663, 1062
TaqI	TCGA	9 cut(s)	426, 449, 510, 612, 699, 888, 906, 971, 1080
TaqII	GACCGA	1 cut(s)	467
TasI	AATT	6 cut(s)	433, 465, 985, 1150, 1568, 1626
TatI	WGTACW	2 cut(s)	711, 990
TauI	GCSGC	7 cut(s)	38, 227, 320, 404, 691, 860, 1145
TfiI	GAWTC	2 cut(s)	908, 961
Tru1I	TTAA	2 cut(s)	51, 72
Tru9I	TTAA	2 cut(s)	51, 72
TscAI	CASTG	5 cut(s)	641, 1018, 1197, 1394, 1599
TseFI	GTSAC	2 cut(s)	205, 1441
Tsp45I	GTSAC	2 cut(s)	205, 1441
TspDTI	ATGAA	3 cut(s)	1046, 1421, 1673
TspGWI	ACGGA	3 cut(s)	238, 355, 749
TspRI	CASTG	5 cut(s)	641, 1018, 1197, 1394, 1599
Tth111I	GACNNNGTC	1 cut(s)	284
Vha464I	CTTAAG	1 cut(s)	71
VpaK11BI	GGWCC	2 cut(s)	892, 1426
XapI	RAATTY	1 cut(s)	985
XbaI	TCTAGA	1 cut(s)	1063
XceI	RCATGY	2 cut(s)	787, 1261
XhoI	CTCGAG	1 cut(s)	509
XmiI	GTMKAC	1 cut(s)	449
XspI	CTAG	4 cut(s)	936, 1064, 1563, 1674

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.