Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0003985

Physical Location & Seq

Genomic Coordinates Reverse (-)

8698226 .. 8700901

Gene Structure 2676 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016668

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGGACGTTCGCCGGCGACCCCCACTCCCTCCCCGCTCCTTCGGCGGCGAACCCATCAAGCAGCACCAGAGAAATCACCCCAAGGCCTCCGACGCGCTGCCGTTGCCGTTGTACTTGACCAACGGCTTGTTCTTCACGCTTTTCTTCACCGTCGCTTACTTCCTCCTCCACCGCTGGCGCGAGAAGATCCGCACCTCCATTCCCCTCCACGTCGTCACGCTCTCAGAGATCGCTGCCCTCTTCTCCCTCTTCGCCTCCGTCATCTACCTCCTCGGCTTCTTCGGCATTGACTTCGTCCAGTCCTTCGTTGGCCTCCGCGCTCCGCACGACCCCTGGAACGACGTCGTAAAAGCGCGGCGCCCCCGAAACGATGTCGTAAGGCCTCCACGCCCCGGTAACGACGTCGTAAAGGGGTCGCAGTTAGTGCCGCAGTGCGAGAACCAGCGCTGCGGCACTAACTGTCCTCCTCCTGTTTCGGTACTGCCCGAGCAGGAGGACGAGGAGATAATTAATTCCGTAGTGTCGGGAGCGACGCCGTCTCACACTCTGGAGTCGAATCTCGGGGACTGCCGGAGAGCAGCCGTCATAAGGCGGGAGGCCCTGCAGCGAAGGACTAGTCGTTCGCTGGAGGGACTGCCCTTGGAAGGCTTCGATTACGACTCTATACTCGGACAGTGCTGCGAGATGCCGATTGGGTATGTGCAGATTCCGGTGGGAGTCGCCGGACCTCTGCTGCTCGACGGCGAGGAGCTCACTATCCCAATGGCGACCACCGAGGGCTGCCTCGTCGCCAGCACCAACCGAGGCTGCAAGGCCATTTACGCTTCCGGTGGGGCCCACAGCGTCATTCTCAAGGACGGCATGACCAGAGCTCCTGTCGTGAGGTTCAACTCCGCCTTCAGAGCCGCCGAGCTCAAGTTCTTCCTGGAGGACCCGGAAAACTTCGATTCTGTTGCCATCGTTTTCAACAGATCTAGTAGATTTGCAAAGCTTCAGGAGATCAAATGCGCGGTTGCAGGCAAGAATCTGTTCATGAGGTTCACATGCAGCACTGGAGATGCGATGGGGATGAACATGGTCTCCAAAGGGGTCCAAAATGTGTTGGATTTTCTCCAGAGTGACTTCCCCGACATGGATGTTATCGGCATCTCCGGGAATTTCTGCTCAGACAAGAAACCGGCAGCGGTGAATTGGATAGAAGGGCGAGGTAAATCAGTAGTGTGCGAGACTGTGATCAATGAAGAAGTGGTGAGGAAGGTACTGAAGACCAATGTAGCTGCCTTGGTGGAGCTCAACATGCTTAAAAACCTTACCGGGTCTGCAATGGCCGGTGCTCTTGGCGGGTTCAATGCCCACGCCAGCAACATTGTCAGTGCAGTGTACATAGCTACCGGCCAGGACCCTGCTCAGAATGTGGAGAGCTCTCACTGCATTACCATGATGGAGGCCATAAACGATGGAAAGGACCTACATGTCTCAGTCACCATGCCTTCCATTGAGGTGGGAACAGTTGGAGGTGGAACACAACTAGCATCACAGTCAGCCTGCCTGAACTTACTGGGAGTTAAGGGTGCCAGCAAGGACTCTCCTGGTTCAAACTCAAGGAAACTAGCCACCATTGTTGCAGGTTCTGTTCTAGCAGGAGAGCTCTCTCTCATGTCAGCCCTTGCAGCAGGTCAGCTAGTTAACAGTCACATGAAATACAACAGGTCTAGTAAGGATGTGTCAAAACTTGCCTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004420 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0006720 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0009058 GO:0009987 GO:0012505 GO:0016020 GO:0016125 GO:0016126 GO:0016491 GO:0016614 GO:0016616 GO:0031090 GO:0031903 GO:0031984 GO:0042175 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0071704 GO:0098588 GO:0098805 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00900 ko01100 ko01110 ko01130 ko04152 ko04976 map00900 map01100 map01110 map01130 map04152 map04976

Pfam Domains

Protein Families

HMG-CoA_red

Protein Analysis

581

Amino Acids

62.55

Weight (kDa)

8.12

Isoelectric Point (pI)

43.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMG-CoA_red	PF00368	195 - 570	1.5e-146	Hydroxymethylglutaryl-coenzyme A reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003985)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G76490
fragaria_vesca	FvH4_1g09980 FvH4_5g26180
malus_domestica	MD02G1108500.v1.1 MD02G1108700.v1.1 MD04G1238500.v1.1 MD08G1025200.v1.1 MD15G1227900.v1.1
prunus_persica	Prupe.7G187000_v2.0.a1 Prupe.7G187500_v2.0.a1
pyrus_communis	pycom02g08710 pycom02g08720 pycom15g20270
rosa_chinensis	RchiOBHm_Chr2g0096771 RchiOBHm_Chr7g0216871
rosa_laevigata	RLG00000002531 RLG00000016668
rosa_multiflora	Rmu_sc0000854.1_g000004 Rmu_sc0004291.1_g000010 Rmu_sc0030893.1_g000001
rosa_roxburghii	Rroxscaffold_2G00145340 Rroxscaffold_3G00242800
rosa_rugosa	Rorug02G0060600 Rorug07G0164000.1
rosa_samantha	Rh2AG107800 Rh2BG110200 Rh2CG112100 Rh2DG111500 Rh7AG303400 Rh7BG295400 Rh7CG322100
rosa_wichuraiana	Rw2G008350 Rw7G025820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1472
AatII	GACGTC	2 cut(s)	345, 405
Acc36I	ACCTGC	2 cut(s)	1616, 1664
AccB1I	GGYRCC	2 cut(s)	357, 1571
AccBSI	CCGCTC	1 cut(s)	36
AccII	CGCG	5 cut(s)	95, 180, 318, 355, 1010
AclWI	GGATC	1 cut(s)	181
AcoI	YGGCCR	2 cut(s)	1326, 1393
AcsI	RAATTY	1 cut(s)	1156
AcuI	CTGAAG	3 cut(s)	883, 977, 1283
AcyI	GRCGYC	4 cut(s)	342, 358, 402, 533
AfaI	GTAC	4 cut(s)	113, 480, 1260, 1382
AfeI	AGCGCT	1 cut(s)	446
AfiI	CCNNNNNNNGG	4 cut(s)	392, 588, 644, 1132
AflIII	ACRYGT	1 cut(s)	1471
AgsI	TTSAA	4 cut(s)	889, 967, 1348, 1596
AhlI	ACTAGT	1 cut(s)	614
AjiI	CACGTC	1 cut(s)	211
AjnI	CCWGG	4 cut(s)	332, 924, 1395, 1588
AjuI	GAANNNNNNNTTGG	2 cut(s)	113, 145
AloI	GAACNNNNNNTCC	4 cut(s)	604, 636, 1064, 1096
Alw21I	GWGCWC	7 cut(s)	753, 874, 915, 1293, 1336, 1424, 1650
Alw26I	GTCTC	4 cut(s)	543, 1084, 1220, 1480
AlwI	GGATC	1 cut(s)	181
AlwNI	CAGNNNCTG	1 cut(s)	1631
Ama87I	CYCGRG	2 cut(s)	485, 560
Aor51HI	AGCGCT	1 cut(s)	446
AoxI	GGCC	9 cut(s)	84, 310, 380, 597, 813, 834, 1326, 1393, 1446
ApaI	GGGCCC	1 cut(s)	838
ApoI	RAATTY	1 cut(s)	1156
ArsI	GACNNNNNNTTYG	4 cut(s)	601, 604, 633, 636
AseI	ATTAAT	1 cut(s)	510
Asp700I	GAANNNNTTC	1 cut(s)	1028
AspLEI	GCGC	7 cut(s)	97, 180, 320, 355, 360, 447, 1010
AspS9I	GGNCC	8 cut(s)	598, 725, 834, 835, 931, 1090, 1399, 1465
AsuC2I	CCSGG	4 cut(s)	393, 935, 1153, 1315
AsuHPI	GGTGA	5 cut(s)	68, 139, 1198, 1261, 1474
AvaI	CYCGRG	2 cut(s)	485, 560
AvaII	GGWCC	5 cut(s)	725, 931, 1090, 1399, 1465
BaeGI	GKGCMC	1 cut(s)	838
BanI	GGYRCC	2 cut(s)	357, 1571
BanII	GRGCYC	7 cut(s)	753, 838, 874, 915, 1293, 1424, 1650
BbsI	GAAGAC	1 cut(s)	1271
Bbv12I	GWGCWC	7 cut(s)	753, 874, 915, 1293, 1336, 1424, 1650
BccI	CCATC	5 cut(s)	62, 965, 1057, 1435, 1451
BceAI	ACGGC	7 cut(s)	85, 91, 139, 520, 566, 757, 874
BcgI	CGANNNNNNTGC	4 cut(s)	274, 308, 935, 969
BciT130I	CCWGG	4 cut(s)	334, 926, 1397, 1590
BclI	TGATCA	1 cut(s)	1233
BcnI	CCSGG	4 cut(s)	393, 935, 1153, 1315
BcoDI	GTCTC	4 cut(s)	543, 1084, 1220, 1480
BcuI	ACTAGT	1 cut(s)	614
BfaI	CTAG	7 cut(s)	615, 975, 1529, 1610, 1637, 1682, 1713
BfmI	CTRYAG	1 cut(s)	602
BfoI	RGCGCY	2 cut(s)	361, 448
BfuAI	ACCTGC	2 cut(s)	1616, 1664
BglII	AGATCT	1 cut(s)	971
Bme1390I	CCNGG	8 cut(s)	334, 393, 926, 935, 1153, 1315, 1397, 1590
Bme18I	GGWCC	5 cut(s)	725, 931, 1090, 1399, 1465
BmeT110I	CYCGRG	2 cut(s)	485, 560
BmgBI	CACGTC	1 cut(s)	211
BmgT120I	GGNCC	8 cut(s)	598, 725, 834, 835, 931, 1090, 1399, 1465
BmiI	GGNNCC	7 cut(s)	359, 835, 836, 933, 1091, 1401, 1573
BmrFI	CCNGG	8 cut(s)	334, 393, 926, 935, 1153, 1315, 1397, 1590
BmrI	ACTGGG	1 cut(s)	1568
BmsI	GCATC	4 cut(s)	675, 1048, 1155, 1541
BmuI	ACTGGG	1 cut(s)	1568
BpiI	GAAGAC	1 cut(s)	1271
BpmI	CTGGAG	5 cut(s)	569, 647, 947, 1074, 1097
BpuEI	CTTGAG	3 cut(s)	836, 899, 1585
BpuMI	CCSGG	4 cut(s)	393, 935, 1153, 1315
BsaHI	GRCGYC	4 cut(s)	342, 358, 402, 533
BsaI	GGTCTC	1 cut(s)	1084
BsaJI	CCNNGG	8 cut(s)	81, 271, 332, 391, 639, 774, 802, 1281
BsaWI	WCCGGW	2 cut(s)	709, 827
BsaXI	ACNNNNNCTCC	6 cut(s)	9, 39, 856, 886, 1064, 1094
Bsc4I	CCNNNNNNNGG	4 cut(s)	392, 588, 644, 1132
Bse118I	RCCGGY	4 cut(s)	12, 1177, 1328, 1391
Bse1I	ACTGG	3 cut(s)	298, 1057, 1563
Bse3DI	GCAATG	1 cut(s)	1329
BseBI	CCWGG	4 cut(s)	334, 926, 1397, 1590
BseDI	CCNNGG	8 cut(s)	81, 271, 332, 391, 639, 774, 802, 1281
BseGI	GGATG	3 cut(s)	1074, 1141, 1726
BseLI	CCNNNNNNNGG	4 cut(s)	392, 588, 644, 1132
BseMI	GCAATG	1 cut(s)	1329
BseMII	CTCAG	4 cut(s)	237, 1179, 1421, 1491
BseNI	ACTGG	3 cut(s)	298, 1057, 1563
BseRI	GAGGAG	5 cut(s)	155, 260, 456, 515, 761
BseSI	GKGCMC	1 cut(s)	838
BsgI	GTGCAG	2 cut(s)	722, 1395
Bsh1236I	CGCG	5 cut(s)	95, 180, 318, 355, 1010
BshFI	GGCC	9 cut(s)	86, 312, 382, 599, 815, 836, 1328, 1395, 1448
BshNI	GGYRCC	2 cut(s)	357, 1571
BsiHKAI	GWGCWC	7 cut(s)	753, 874, 915, 1293, 1336, 1424, 1650
BsiHKCI	CYCGRG	2 cut(s)	485, 560
BslFI	GGGAC	2 cut(s)	578, 645
BslI	CCNNNNNNNGG	4 cut(s)	392, 588, 644, 1132
BsmAI	GTCTC	4 cut(s)	543, 1084, 1220, 1480
BsmBI	CGTCTC	1 cut(s)	543
BsmFI	GGGAC	2 cut(s)	578, 645
BsnI	GGCC	9 cut(s)	86, 312, 382, 599, 815, 836, 1328, 1395, 1448
Bso31I	GGTCTC	1 cut(s)	1084
BsoBI	CYCGRG	2 cut(s)	485, 560
Bsp120I	GGGCCC	1 cut(s)	834
Bsp1286I	GDGCHC	8 cut(s)	753, 838, 874, 915, 1293, 1336, 1424, 1650
Bsp1407I	TGTACA	1 cut(s)	1380
Bsp143I	GATC	5 cut(s)	186, 228, 971, 999, 1233
BspANI	GGCC	9 cut(s)	86, 312, 382, 599, 815, 836, 1328, 1395, 1448
BspCNI	CTCAG	4 cut(s)	236, 1178, 1420, 1490
BspFNI	CGCG	5 cut(s)	95, 180, 318, 355, 1010
BspHI	TCATGA	1 cut(s)	1032
BspLI	GGNNCC	7 cut(s)	359, 835, 836, 933, 1091, 1401, 1573
BspMAI	CTGCAG	1 cut(s)	606
BspMI	ACCTGC	2 cut(s)	1616, 1664
BspPI	GGATC	1 cut(s)	181
BspT107I	GGYRCC	2 cut(s)	357, 1571
BspTNI	GGTCTC	1 cut(s)	1084
BsrBI	CCGCTC	1 cut(s)	36
BsrDI	GCAATG	1 cut(s)	1329
BsrFI	RCCGGY	4 cut(s)	12, 1177, 1328, 1391
BsrGI	TGTACA	1 cut(s)	1380
BsrI	ACTGG	3 cut(s)	298, 1057, 1563
BssAI	RCCGGY	4 cut(s)	12, 1177, 1328, 1391
BssECI	CCNNGG	8 cut(s)	81, 271, 332, 391, 639, 774, 802, 1281
BssMI	GATC	5 cut(s)	186, 228, 971, 999, 1233
BssNI	GRCGYC	4 cut(s)	342, 358, 402, 533
BssT1I	CCWWGG	3 cut(s)	81, 639, 1281
Bst2UI	CCWGG	4 cut(s)	334, 926, 1397, 1590
Bst4CI	ACNGT	7 cut(s)	151, 461, 675, 1231, 1510, 1539, 1691
Bst6I	CTCTTC	2 cut(s)	245, 254
BstACI	GRCGYC	4 cut(s)	342, 358, 402, 533
BstAPI	GCANNNNNTGC	1 cut(s)	424
BstAUI	TGTACA	1 cut(s)	1380
BstC8I	GCNNGC	8 cut(s)	14, 176, 793, 1018, 1360, 1546, 1576, 1738
BstDEI	CTNAG	4 cut(s)	223, 1165, 1407, 1477
BstF5I	GGATG	3 cut(s)	1074, 1141, 1726
BstFNI	CGCG	5 cut(s)	95, 180, 318, 355, 1010
BstH2I	RGCGCY	2 cut(s)	361, 448
BstHHI	GCGC	7 cut(s)	97, 180, 320, 355, 360, 447, 1010
BstKTI	GATC	5 cut(s)	189, 231, 974, 1002, 1236
BstMAI	GTCTC	4 cut(s)	543, 1084, 1220, 1480
BstMBI	GATC	5 cut(s)	186, 228, 971, 999, 1233
BstNI	CCWGG	4 cut(s)	334, 926, 1397, 1590
BstNSI	RCATGY	3 cut(s)	1047, 1300, 1475
BstSCI	CCNGG	8 cut(s)	332, 391, 924, 933, 1151, 1313, 1395, 1588
BstSFI	CTRYAG	1 cut(s)	602
BstSLI	GKGCMC	1 cut(s)	838
BstUI	CGCG	5 cut(s)	95, 180, 318, 355, 1010
BstV2I	GAAGAC	1 cut(s)	1271
BstX2I	RGATCY	2 cut(s)	186, 971
BstXI	CCANNNNNNTGG	1 cut(s)	1501
BstYI	RGATCY	2 cut(s)	186, 971
BsuRI	GGCC	9 cut(s)	86, 312, 382, 599, 815, 836, 1328, 1395, 1448
BtgZI	GCGATG	1 cut(s)	1076
BtrI	CACGTC	1 cut(s)	211
BtsCI	GGATG	3 cut(s)	1074, 1141, 1726
BtsI	GCAGTG	3 cut(s)	437, 1383, 1426
BtsIMutI	CAGTG	6 cut(s)	437, 680, 1050, 1378, 1383, 1426
BveI	ACCTGC	2 cut(s)	1616, 1664
Cac8I	GCNNGC	8 cut(s)	14, 176, 793, 1018, 1360, 1546, 1576, 1738
CaiI	CAGNNNCTG	1 cut(s)	1631
CciI	TCATGA	1 cut(s)	1032
CfoI	GCGC	7 cut(s)	97, 180, 320, 355, 360, 447, 1010
Cfr10I	RCCGGY	4 cut(s)	12, 1177, 1328, 1391
Cfr13I	GGNCC	8 cut(s)	598, 725, 834, 835, 931, 1090, 1399, 1465
CseI	GACGC	3 cut(s)	101, 541, 832
Csp6I	GTAC	4 cut(s)	112, 479, 1259, 1381
CspCI	CAANNNNNGTGG	2 cut(s)	1603, 1638
CviQI	GTAC	4 cut(s)	112, 479, 1259, 1381
DdeI	CTNAG	4 cut(s)	223, 1165, 1407, 1477
DinI	GGCGCC	1 cut(s)	359
DpnI	GATC	5 cut(s)	188, 230, 973, 1001, 1235
DpnII	GATC	5 cut(s)	186, 228, 971, 999, 1233
DrdI	GACNNNNNNGTC	1 cut(s)	1472
DseDI	GACNNNNNNGTC	1 cut(s)	1472
EaeI	YGGCCR	2 cut(s)	1326, 1393
Eam1104I	CTCTTC	2 cut(s)	245, 254
EarI	CTCTTC	2 cut(s)	245, 254
EciI	GGCGGA	1 cut(s)	883
Ecl136II	GAGCTC	6 cut(s)	751, 872, 913, 1291, 1422, 1648
Eco130I	CCWWGG	3 cut(s)	81, 639, 1281
Eco147I	AGGCCT	2 cut(s)	86, 382
Eco24I	GRGCYC	7 cut(s)	753, 838, 874, 915, 1293, 1424, 1650
Eco31I	GGTCTC	1 cut(s)	1084
Eco47I	GGWCC	5 cut(s)	725, 931, 1090, 1399, 1465
Eco47III	AGCGCT	1 cut(s)	446
Eco53kI	GAGCTC	6 cut(s)	751, 872, 913, 1291, 1422, 1648
Eco57I	CTGAAG	3 cut(s)	883, 977, 1283
Eco88I	CYCGRG	2 cut(s)	485, 560
EcoICRI	GAGCTC	6 cut(s)	751, 872, 913, 1291, 1422, 1648
EcoO109I	RGGNCCY	5 cut(s)	598, 834, 931, 1399, 1465
EcoRII	CCWGG	4 cut(s)	332, 924, 1395, 1588
EcoT14I	CCWWGG	3 cut(s)	81, 639, 1281
EcoT38I	GRGCYC	7 cut(s)	753, 838, 874, 915, 1293, 1424, 1650
EgeI	GGCGCC	1 cut(s)	359
EheI	GGCGCC	1 cut(s)	359
ErhI	CCWWGG	3 cut(s)	81, 639, 1281
Esp3I	CGTCTC	1 cut(s)	543
FaqI	GGGAC	2 cut(s)	578, 645
FauI	CCCGC	3 cut(s)	41, 585, 1334
FbaI	TGATCA	1 cut(s)	1233
FokI	GGATG	3 cut(s)	1081, 1148, 1733
FriOI	GRGCYC	7 cut(s)	753, 838, 874, 915, 1293, 1424, 1650
FspBI	CTAG	7 cut(s)	615, 975, 1529, 1610, 1637, 1682, 1713
GlaI	GCGC	7 cut(s)	96, 179, 319, 354, 359, 446, 1009
GsuI	CTGGAG	5 cut(s)	569, 647, 947, 1074, 1097
HaeII	RGCGCY	2 cut(s)	361, 448
HaeIII	GGCC	9 cut(s)	86, 312, 382, 599, 815, 836, 1328, 1395, 1448
HgaI	GACGC	3 cut(s)	101, 541, 832
HhaI	GCGC	7 cut(s)	97, 180, 320, 355, 360, 447, 1010
Hin1I	GRCGYC	4 cut(s)	342, 358, 402, 533
Hin6I	GCGC	7 cut(s)	95, 178, 318, 353, 358, 445, 1008
HinP1I	GCGC	7 cut(s)	95, 178, 318, 353, 358, 445, 1008
HincII	GTYRAC	1 cut(s)	1687
HindII	GTYRAC	1 cut(s)	1687
HindIII	AAGCTT	1 cut(s)	989
HinfI	GANTC	8 cut(s)	551, 556, 659, 706, 717, 947, 1024, 1583
HpaI	GTTAAC	1 cut(s)	1687
HphI	GGTGA	5 cut(s)	68, 139, 1198, 1261, 1474
Hpy166II	GTNNAC	3 cut(s)	1041, 1381, 1687
Hpy188I	TCNGA	6 cut(s)	91, 226, 671, 902, 1168, 1410
Hpy188III	TCNNGA	6 cut(s)	525, 548, 880, 995, 1033, 1114
Hpy8I	GTNNAC	3 cut(s)	1041, 1381, 1687
HpyAV	CCTTC	8 cut(s)	49, 313, 603, 638, 907, 1193, 1249, 1500
HpyCH4III	ACNGT	7 cut(s)	151, 461, 675, 1231, 1510, 1539, 1691
HpyCH4IV	ACGT	4 cut(s)	6, 210, 342, 402
HpyF3I	CTNAG	4 cut(s)	223, 1165, 1407, 1477
HpySE526I	ACGT	4 cut(s)	6, 210, 342, 402
Hsp92I	GRCGYC	4 cut(s)	342, 358, 402, 533
HspAI	GCGC	7 cut(s)	95, 178, 318, 353, 358, 445, 1008
KasI	GGCGCC	1 cut(s)	357
KroI	GCCGGC	1 cut(s)	12
KroNI	GCCGGC	1 cut(s)	14
Ksp22I	TGATCA	1 cut(s)	1233
KspAI	GTTAAC	1 cut(s)	1687
Kzo9I	GATC	5 cut(s)	186, 228, 971, 999, 1233
LmnI	GCTCC	6 cut(s)	41, 325, 527, 748, 877, 1288
LweI	GCATC	4 cut(s)	675, 1048, 1155, 1541
MaeI	CTAG	7 cut(s)	615, 975, 1529, 1610, 1637, 1682, 1713
MaeII	ACGT	4 cut(s)	6, 210, 342, 402
MaeIII	GTNAC	5 cut(s)	214, 395, 1118, 1480, 1691
MalI	GATC	5 cut(s)	188, 230, 973, 1001, 1235
MbiI	CCGCTC	1 cut(s)	36
MboI	GATC	5 cut(s)	186, 228, 971, 999, 1233
MboII	GAAGA	9 cut(s)	124, 136, 196, 232, 241, 271, 913, 1253, 1276
MflI	RGATCY	2 cut(s)	186, 971
MhlI	GDGCHC	8 cut(s)	753, 838, 874, 915, 1293, 1336, 1424, 1650
MluCI	AATT	4 cut(s)	507, 511, 1156, 1189
Mly113I	GGCGCC	1 cut(s)	358
MlyI	GAGTC	4 cut(s)	560, 653, 726, 1577
MmeI	TCCRAC	3 cut(s)	114, 1083, 1492
MreI	CGCCGGCG	1 cut(s)	12
MroNI	GCCGGC	1 cut(s)	12
MroXI	GAANNNNTTC	1 cut(s)	1028
MseI	TTAA	4 cut(s)	510, 1302, 1566, 1686
MslI	CAYNNNNRTG	2 cut(s)	1436, 1499
MspA1I	CMGCKG	2 cut(s)	174, 1184
MspR9I	CCNGG	8 cut(s)	334, 393, 926, 935, 1153, 1315, 1397, 1590
MteI	GCGCNGCGC	1 cut(s)	356
MvaI	CCWGG	4 cut(s)	334, 926, 1397, 1590
MvnI	CGCG	5 cut(s)	95, 180, 318, 355, 1010
NaeI	GCCGGC	1 cut(s)	14
NarI	GGCGCC	1 cut(s)	358
NciI	CCSGG	4 cut(s)	393, 935, 1153, 1315
NdeII	GATC	5 cut(s)	186, 228, 971, 999, 1233
NgoMIV	GCCGGC	1 cut(s)	12
NlaIV	GGNNCC	7 cut(s)	359, 835, 836, 933, 1091, 1401, 1573
NmeAIII	GCCGAG	2 cut(s)	252, 934
NmuCI	GTSAC	4 cut(s)	214, 1118, 1480, 1691
NspI	RCATGY	3 cut(s)	1047, 1300, 1475
PagI	TCATGA	1 cut(s)	1032
PceI	AGGCCT	2 cut(s)	86, 382
PciI	ACATGT	1 cut(s)	1471
PdiI	GCCGGC	1 cut(s)	14
PdmI	GAANNNNTTC	1 cut(s)	1028
PfeI	GAWTC	4 cut(s)	556, 706, 947, 1024
PflFI	GACNNNGTC	2 cut(s)	293, 535
PfoI	TCCNGGA	2 cut(s)	924, 1151
PleI	GAGTC	4 cut(s)	559, 653, 725, 1577
PluTI	GGCGCC	1 cut(s)	361
PpsI	GAGTC	4 cut(s)	559, 653, 725, 1577
PpuMI	RGGWCCY	3 cut(s)	931, 1399, 1465
PscI	ACATGT	1 cut(s)	1471
PshBI	ATTAAT	1 cut(s)	510
Psp124BI	GAGCTC	6 cut(s)	753, 874, 915, 1293, 1424, 1650
Psp5II	RGGWCCY	3 cut(s)	931, 1399, 1465
Psp6I	CCWGG	4 cut(s)	332, 924, 1395, 1588
PspGI	CCWGG	4 cut(s)	332, 924, 1395, 1588
PspN4I	GGNNCC	7 cut(s)	359, 835, 836, 933, 1091, 1401, 1573
PspOMI	GGGCCC	1 cut(s)	834
PspPI	GGNCC	8 cut(s)	598, 725, 834, 835, 931, 1090, 1399, 1465
PspPPI	RGGWCCY	3 cut(s)	931, 1399, 1465
PstI	CTGCAG	1 cut(s)	606
PstNI	CAGNNNCTG	1 cut(s)	1631
PsuI	RGATCY	2 cut(s)	186, 971
PsyI	GACNNNGTC	2 cut(s)	293, 535
RsaI	GTAC	4 cut(s)	113, 480, 1260, 1382
RsaNI	GTAC	4 cut(s)	112, 479, 1259, 1381
RseI	CAYNNNNRTG	2 cut(s)	1436, 1499
SacI	GAGCTC	6 cut(s)	753, 874, 915, 1293, 1424, 1650
SaqAI	TTAA	4 cut(s)	510, 1302, 1566, 1686
Sau3AI	GATC	5 cut(s)	186, 228, 971, 999, 1233
Sau96I	GGNCC	8 cut(s)	598, 725, 834, 835, 931, 1090, 1399, 1465
SchI	GAGTC	4 cut(s)	560, 653, 726, 1577
ScrFI	CCNGG	8 cut(s)	334, 393, 926, 935, 1153, 1315, 1397, 1590
SduI	GDGCHC	8 cut(s)	753, 838, 874, 915, 1293, 1336, 1424, 1650
SfaNI	GCATC	4 cut(s)	675, 1048, 1155, 1541
SfcI	CTRYAG	1 cut(s)	602
SfoI	GGCGCC	1 cut(s)	359
SgrAI	CRCCGGYG	1 cut(s)	12
SinI	GGWCC	5 cut(s)	725, 931, 1090, 1399, 1465
SmiMI	CAYNNNNRTG	2 cut(s)	1436, 1499
SmlI	CTYRAG	3 cut(s)	851, 914, 1600
SmoI	CTYRAG	3 cut(s)	851, 914, 1600
SpeI	ACTAGT	1 cut(s)	614
Sse9I	AATT	4 cut(s)	507, 511, 1156, 1189
SseBI	AGGCCT	2 cut(s)	86, 382
SspDI	GGCGCC	1 cut(s)	357
SspMI	CTAG	7 cut(s)	615, 975, 1529, 1610, 1637, 1682, 1713
SstI	GAGCTC	6 cut(s)	753, 874, 915, 1293, 1424, 1650
StuI	AGGCCT	2 cut(s)	86, 382
StyD4I	CCNGG	8 cut(s)	332, 391, 924, 933, 1151, 1313, 1395, 1588
StyI	CCWWGG	3 cut(s)	81, 639, 1281
TaaI	ACNGT	7 cut(s)	151, 461, 675, 1231, 1510, 1539, 1691
TaiI	ACGT	4 cut(s)	9, 213, 345, 405
TaqI	TCGA	4 cut(s)	554, 651, 738, 945
TasI	AATT	4 cut(s)	507, 511, 1156, 1189
TatI	WGTACW	2 cut(s)	111, 1380
TauI	GCSGC	5 cut(s)	48, 358, 430, 453, 908
TfiI	GAWTC	4 cut(s)	556, 706, 947, 1024
Tru1I	TTAA	4 cut(s)	510, 1302, 1566, 1686
Tru9I	TTAA	4 cut(s)	510, 1302, 1566, 1686
TscAI	CASTG	6 cut(s)	437, 680, 1057, 1378, 1383, 1433
TseFI	GTSAC	4 cut(s)	214, 1118, 1480, 1691
Tsp45I	GTSAC	4 cut(s)	214, 1118, 1480, 1691
TspDTI	ATGAA	4 cut(s)	1021, 1085, 1254, 1712
TspGWI	ACGGA	2 cut(s)	247, 505
TspRI	CASTG	6 cut(s)	437, 680, 1057, 1378, 1383, 1433
Tth111I	GACNNNGTC	2 cut(s)	293, 535
VpaK11BI	GGWCC	5 cut(s)	725, 931, 1090, 1399, 1465
VspI	ATTAAT	1 cut(s)	510
XapI	RAATTY	1 cut(s)	1156
XceI	RCATGY	3 cut(s)	1047, 1300, 1475
XcmI	CCANNNNNNNNNTGG	1 cut(s)	305
XmnI	GAANNNNTTC	1 cut(s)	1028
XspI	CTAG	7 cut(s)	615, 975, 1529, 1610, 1637, 1682, 1713
ZraI	GACGTC	2 cut(s)	343, 403

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.