Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003203

Physical Location & Seq

Genomic Coordinates Forward (+)

11981521 .. 11983005

Gene Structure 1485 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1144800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGAATGATTTGATGACCAAATCTTTCTTAAGTTATGTGGAACTGAAGAAACAGGCGCAGAAGGACCTCGAAGCGGATCTTGATGTAGAAGCAGGCGCAGGCAAACTCAACCCGTCCGACCAAGAAAACCTCTCCCAGTTCTTCCAAGAAGTCGGAGCAATCAAGGCCGACATGGAAGAGATCACCAATCTCTTGCACGATCTCCAGAGCCTGAACGCAGAGGCAAAGTCCACTCACAGTGCCAAGATTCTCCGCGGTCTTAGAGACCGGATGGAATCTGACATGGTCGCAGTGCTTCGAAAAGCGAGGATTGTCAAGGCGAGGCTTGAGGCGCTTGATCAGTCAAATGCGACCAACAGAAAAATGTCCGATGCGTATAAGGAAGGAAGCACTGTGGACCGGACTAGAATGTCCATTACTGGTGGCTTGAGAGTCAAGCTAAGGGACCTTATGGACAATTTTCAATCTCTGAGAGAGAAGATTGTTTCGGATCATAAGGAAGATCTGAAGAGGAGGTACTACAGCGCAACGGGCGACTTGCCATCCGATGAGATGATCGAAAAGATAATTTCCGGAAGCTTGAAAGTTGAATTGTTCGAAGGGAAGACGGAACTGGATATTGGGAACAAAGTCCGACACGAGGCAGTGATGGATATCCAGAGAAGTCTGACAAAGCTTCATCAGGTGTTTCTCGACATGGCTGTTCTGGTTGAAGCACAAGGCGAAGACATTGAAAATATCGAAGACAATGTGGCGAATGCCAGTTATTTCATCAGTGGTGGCACAAACAGTCTTCACCATGCCAGCCAGATGAAGAAGAAGAACAAGAAGTGGGTTTACTGGGTGTGGGCTGTGGGCCTGATTATACTTCTGGTTTGCATTATTTCGATGTTTGCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006810 GO:0006887 GO:0006906 GO:0006950 GO:0006996 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0031201 GO:0031224 GO:0032940 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0046903 GO:0048278 GO:0048284 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0061024 GO:0061025 GO:0071840 GO:0071944 GO:0090174 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko00770 ko01100 ko04130 ko04721 map00770 map01100 map04130 map04721

Pfam Domains

Protein Families

CTP_transf_like SNARE Syntaxin

Protein Analysis

301

Amino Acids

33.91

Weight (kDa)

6.03

Isoelectric Point (pI)

36.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin	PF00804	42 - 199	4e-44	Syntaxin
SNARE	PF05739	244 - 292	1.4e-07	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003203)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18260 AT2G18260
fragaria_vesca	FvH4_1g13020 FvH4_2g09080 FvH4_2g09080
malus_domestica	MD02G1144800.v1.1 MD05G1064700.v1.1 MD15G1259000.v1.1
prunus_persica	Prupe.7G156800_v2.0.a1 Prupe.8G094700_v2.0.a1 Prupe.8G094700_v2.0.a1
pyrus_communis	pycom02g11470 pycom05g05560 pycom15g22740
rosa_chinensis	RchiOBHm_Chr2g0101181 RchiOBHm_Chr6g0264961
rosa_laevigata	RLG00000014227 RLG00000017053
rosa_multiflora	Rmu_co8052262.1_g000001 Rmu_sc0002934.1_g000038 Rmu_sc0006314.1_g000005
rosa_roxburghii	Rroxscaffold_175G00432060 Rroxscaffold_2G00141520 Rroxscaffold_7G00203130
rosa_rugosa	Rorug02G0100400 Rorug02G0100500 Rorug06G0014100
rosa_samantha	Rh2AG144200 Rh2BG149200 Rh2CG150500 Rh2DG149700 Rh6AG135300 Rh6BG132700 Rh6CG131400 Rh6DG118400
rosa_wichuraiana	Rw2G011300 Rw6G011690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	409
AccII	CGCG	1 cut(s)	255
AccIII	TCCGGA	1 cut(s)	572
AciI	CCGC	3 cut(s)	74, 253, 255
AclWI	GGATC	2 cut(s)	84, 498
AcuI	CTGAAG	2 cut(s)	65, 527
AfaI	GTAC	1 cut(s)	518
AfiI	CCNNNNNNNGG	2 cut(s)	73, 640
AflII	CTTAAG	1 cut(s)	28
AgsI	TTSAA	5 cut(s)	464, 583, 590, 713, 734
AjuI	GAANNNNNNNTTGG	2 cut(s)	603, 635
AluBI	AGCT	3 cut(s)	439, 579, 676
AluI	AGCT	3 cut(s)	439, 579, 676
Alw26I	GTCTC	1 cut(s)	258
AlwI	GGATC	2 cut(s)	84, 498
AlwNI	CAGNNNCTG	1 cut(s)	211
Aor13HI	TCCGGA	1 cut(s)	572
AoxI	GGCC	2 cut(s)	165, 856
AspLEI	GCGC	4 cut(s)	58, 98, 334, 527
AspS9I	GGNCC	4 cut(s)	64, 397, 445, 856
AsuHPI	GGTGA	2 cut(s)	175, 788
AsuII	TTCGAA	2 cut(s)	298, 597
AvaII	GGWCC	3 cut(s)	64, 397, 445
BarI	GAAGNNNNNNTAC	4 cut(s)	500, 532, 821, 853
BauI	CACGAG	1 cut(s)	638
BbsI	GAAGAC	4 cut(s)	611, 732, 750, 785
BccI	CCATC	3 cut(s)	265, 550, 643
BclI	TGATCA	1 cut(s)	337
BcoDI	GTCTC	1 cut(s)	258
BfaI	CTAG	1 cut(s)	405
BfmI	CTRYAG	1 cut(s)	520
BfoI	RGCGCY	1 cut(s)	335
BfrI	CTTAAG	1 cut(s)	28
BglII	AGATCT	1 cut(s)	502
Bme18I	GGWCC	3 cut(s)	64, 397, 445
BmgT120I	GGNCC	4 cut(s)	64, 397, 445, 856
BmiI	GGNNCC	1 cut(s)	446
BmrI	ACTGGG	2 cut(s)	130, 850
BmsI	GCATC	1 cut(s)	361
BmuI	ACTGGG	2 cut(s)	130, 850
BpiI	GAAGAC	4 cut(s)	611, 732, 750, 785
BpmI	CTGGAG	1 cut(s)	188
Bpu10I	CCTNAGC	1 cut(s)	440
Bpu14I	TTCGAA	2 cut(s)	298, 597
BpuEI	CTTGAG	2 cut(s)	347, 448
BsaBI	GATNNNNATC	1 cut(s)	653
BsaI	GGTCTC	1 cut(s)	258
BsaJI	CCNNGG	1 cut(s)	253
BsaWI	WCCGGW	3 cut(s)	267, 399, 572
Bsc4I	CCNNNNNNNGG	2 cut(s)	73, 640
Bse1I	ACTGG	5 cut(s)	136, 424, 618, 762, 845
Bse8I	GATNNNNATC	1 cut(s)	653
BseAI	TCCGGA	1 cut(s)	572
BseDI	CCNNGG	1 cut(s)	253
BseGI	GGATG	2 cut(s)	276, 542
BseJI	GATNNNNATC	1 cut(s)	653
BseLI	CCNNNNNNNGG	2 cut(s)	73, 640
BseMII	CTCAG	1 cut(s)	461
BseNI	ACTGG	5 cut(s)	136, 424, 618, 762, 845
BseRI	GAGGAG	1 cut(s)	526
Bsh1236I	CGCG	1 cut(s)	255
BshFI	GGCC	2 cut(s)	167, 858
BsiSI	CCGG	3 cut(s)	268, 400, 573
BslFI	GGGAC	1 cut(s)	458
BslI	CCNNNNNNNGG	2 cut(s)	73, 640
BsmAI	GTCTC	1 cut(s)	258
BsmFI	GGGAC	1 cut(s)	458
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	2 cut(s)	167, 858
Bso31I	GGTCTC	1 cut(s)	258
Bsp119I	TTCGAA	2 cut(s)	298, 597
Bsp13I	TCCGGA	1 cut(s)	572
Bsp143I	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
BspACI	CCGC	3 cut(s)	74, 253, 255
BspANI	GGCC	2 cut(s)	167, 858
BspCNI	CTCAG	1 cut(s)	462
BspEI	TCCGGA	1 cut(s)	572
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	1 cut(s)	446
BspPI	GGATC	2 cut(s)	84, 498
BspT104I	TTCGAA	2 cut(s)	298, 597
BspTI	CTTAAG	1 cut(s)	28
BspTNI	GGTCTC	1 cut(s)	258
BsrI	ACTGG	5 cut(s)	136, 424, 618, 762, 845
BssECI	CCNNGG	1 cut(s)	253
BssMI	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
BssSI	CACGAG	1 cut(s)	638
Bst2BI	CACGAG	1 cut(s)	638
Bst4CI	ACNGT	3 cut(s)	239, 394, 791
Bst6I	CTCTTC	2 cut(s)	171, 503
BstAFI	CTTAAG	1 cut(s)	28
BstBI	TTCGAA	2 cut(s)	298, 597
BstC8I	GCNNGC	3 cut(s)	94, 100, 805
BstDEI	CTNAG	3 cut(s)	260, 440, 470
BstDSI	CCRYGG	1 cut(s)	253
BstF5I	GGATG	2 cut(s)	276, 542
BstFNI	CGCG	1 cut(s)	255
BstH2I	RGCGCY	1 cut(s)	335
BstHHI	GCGC	4 cut(s)	58, 98, 334, 527
BstKTI	GATC	7 cut(s)	79, 183, 202, 340, 493, 505, 558
BstMAI	GTCTC	1 cut(s)	258
BstMBI	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
BstMWI	GCNNNNNNNGC	3 cut(s)	164, 331, 531
BstSFI	CTRYAG	1 cut(s)	520
BstUI	CGCG	1 cut(s)	255
BstV2I	GAAGAC	4 cut(s)	611, 732, 750, 785
BstX2I	RGATCY	2 cut(s)	76, 502
BstYI	RGATCY	2 cut(s)	76, 502
BsuRI	GGCC	2 cut(s)	167, 858
BtgI	CCRYGG	1 cut(s)	253
BtsCI	GGATG	2 cut(s)	276, 542
BtsI	GCAGTG	2 cut(s)	297, 651
BtsIMutI	CAGTG	5 cut(s)	244, 297, 390, 651, 781
Cac8I	GCNNGC	3 cut(s)	94, 100, 805
CaiI	CAGNNNCTG	1 cut(s)	211
CfoI	GCGC	4 cut(s)	58, 98, 334, 527
Cfr13I	GGNCC	4 cut(s)	64, 397, 445, 856
Cfr42I	CCGCGG	1 cut(s)	256
Csp6I	GTAC	1 cut(s)	517
CviAII	CATG	4 cut(s)	172, 283, 697, 800
CviQI	GTAC	1 cut(s)	517
DdeI	CTNAG	3 cut(s)	260, 440, 470
DpnI	GATC	7 cut(s)	78, 182, 201, 339, 492, 504, 557
DpnII	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
DrdI	GACNNNNNNGTC	1 cut(s)	409
DseDI	GACNNNNNNGTC	1 cut(s)	409
Eam1104I	CTCTTC	2 cut(s)	171, 503
EarI	CTCTTC	2 cut(s)	171, 503
Eco31I	GGTCTC	1 cut(s)	258
Eco32I	GATATC	1 cut(s)	655
Eco47I	GGWCC	3 cut(s)	64, 397, 445
Eco57I	CTGAAG	2 cut(s)	65, 527
EcoO109I	RGGNCCY	2 cut(s)	64, 445
EcoRV	GATATC	1 cut(s)	655
FaeI	CATG	4 cut(s)	175, 286, 700, 803
FaiI	YATR	9 cut(s)	36, 173, 284, 378, 452, 495, 698, 801, 866
FalI	AAGNNNNNCTT	2 cut(s)	63, 95
FaqI	GGGAC	1 cut(s)	458
FatI	CATG	4 cut(s)	171, 282, 696, 799
FbaI	TGATCA	1 cut(s)	337
FokI	GGATG	2 cut(s)	283, 529
FspBI	CTAG	1 cut(s)	405
GlaI	GCGC	4 cut(s)	57, 97, 333, 526
GsuI	CTGGAG	1 cut(s)	188
HaeII	RGCGCY	1 cut(s)	335
HaeIII	GGCC	2 cut(s)	167, 858
HapII	CCGG	3 cut(s)	268, 400, 573
HhaI	GCGC	4 cut(s)	58, 98, 334, 527
Hin1II	CATG	4 cut(s)	175, 286, 700, 803
Hin6I	GCGC	4 cut(s)	56, 96, 332, 525
HinP1I	GCGC	4 cut(s)	56, 96, 332, 525
HindIII	AAGCTT	2 cut(s)	577, 674
HinfI	GANTC	3 cut(s)	247, 275, 432
HpaII	CCGG	3 cut(s)	268, 400, 573
HphI	GGTGA	2 cut(s)	175, 788
Hpy166II	GTNNAC	3 cut(s)	231, 397, 838
Hpy188III	TCNNGA	5 cut(s)	80, 205, 573, 658, 692
Hpy8I	GTNNAC	3 cut(s)	231, 397, 838
HpyAV	CCTTC	3 cut(s)	55, 377, 593
HpyCH4III	ACNGT	3 cut(s)	239, 394, 791
HpyCH4V	TGCA	2 cut(s)	196, 879
HpyF10VI	GCNNNNNNNGC	3 cut(s)	164, 331, 531
HpyF3I	CTNAG	3 cut(s)	260, 440, 470
Hsp92II	CATG	4 cut(s)	175, 286, 700, 803
HspAI	GCGC	4 cut(s)	56, 96, 332, 525
Kpn2I	TCCGGA	1 cut(s)	572
Ksp22I	TGATCA	1 cut(s)	337
KspI	CCGCGG	1 cut(s)	256
Kzo9I	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
LmnI	GCTCC	1 cut(s)	155
LweI	GCATC	1 cut(s)	361
MaeI	CTAG	1 cut(s)	405
MalI	GATC	7 cut(s)	78, 182, 201, 339, 492, 504, 557
MboI	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
MflI	RGATCY	2 cut(s)	76, 502
MluCI	AATT	3 cut(s)	457, 567, 590
MlyI	GAGTC	1 cut(s)	441
MmeI	TCCRAC	3 cut(s)	133, 141, 658
MnlI	CCTC	9 cut(s)	77, 140, 214, 300, 315, 322, 504, 507, 634
MroI	TCCGGA	1 cut(s)	572
MseI	TTAA	1 cut(s)	29
MspA1I	CMGCKG	1 cut(s)	255
MspCI	CTTAAG	1 cut(s)	28
MspI	CCGG	3 cut(s)	268, 400, 573
Mva1269I	GAATGC	1 cut(s)	763
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	3 cut(s)	164, 331, 531
NdeII	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
NlaIII	CATG	4 cut(s)	175, 286, 700, 803
NlaIV	GGNNCC	1 cut(s)	446
NspV	TTCGAA	2 cut(s)	298, 597
PctI	GAATGC	1 cut(s)	763
PfeI	GAWTC	2 cut(s)	247, 275
PflFI	GACNNNGTC	1 cut(s)	284
PleI	GAGTC	1 cut(s)	440
PpsI	GAGTC	1 cut(s)	440
PpuMI	RGGWCCY	2 cut(s)	64, 445
Psp5II	RGGWCCY	2 cut(s)	64, 445
PspN4I	GGNNCC	1 cut(s)	446
PspPI	GGNCC	4 cut(s)	64, 397, 445, 856
PspPPI	RGGWCCY	2 cut(s)	64, 445
PstNI	CAGNNNCTG	1 cut(s)	211
PsuI	RGATCY	2 cut(s)	76, 502
PsyI	GACNNNGTC	1 cut(s)	284
RsaI	GTAC	1 cut(s)	518
RsaNI	GTAC	1 cut(s)	517
SacII	CCGCGG	1 cut(s)	256
SaqAI	TTAA	1 cut(s)	29
Sau3AI	GATC	7 cut(s)	76, 180, 199, 337, 490, 502, 555
Sau96I	GGNCC	4 cut(s)	64, 397, 445, 856
SchI	GAGTC	1 cut(s)	441
SetI	ASST	8 cut(s)	69, 132, 441, 450, 518, 581, 678, 687
SfaNI	GCATC	1 cut(s)	361
SfcI	CTRYAG	1 cut(s)	520
Sfr303I	CCGCGG	1 cut(s)	256
SfuI	TTCGAA	2 cut(s)	298, 597
SgrBI	CCGCGG	1 cut(s)	256
SinI	GGWCC	3 cut(s)	64, 397, 445
SmlI	CTYRAG	3 cut(s)	28, 326, 427
SmoI	CTYRAG	3 cut(s)	28, 326, 427
Sse9I	AATT	3 cut(s)	457, 567, 590
SsiI	CCGC	3 cut(s)	74, 253, 255
SspMI	CTAG	1 cut(s)	405
TaaI	ACNGT	3 cut(s)	239, 394, 791
TaqI	TCGA	7 cut(s)	69, 298, 558, 597, 693, 741, 887
TasI	AATT	3 cut(s)	457, 567, 590
TfiI	GAWTC	2 cut(s)	247, 275
Tru1I	TTAA	1 cut(s)	29
Tru9I	TTAA	1 cut(s)	29
TscAI	CASTG	5 cut(s)	244, 297, 397, 651, 781
TspDTI	ATGAA	4 cut(s)	17, 668, 760, 827
TspGWI	ACGGA	1 cut(s)	623
TspRI	CASTG	5 cut(s)	244, 297, 397, 651, 781
Tth111I	GACNNNGTC	1 cut(s)	284
Vha464I	CTTAAG	1 cut(s)	28
VpaK11BI	GGWCC	3 cut(s)	64, 397, 445
XspI	CTAG	1 cut(s)	405

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.