MD05G1064700.v1.1

Syntaxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003203
Physical Location & Seq
Reverse (-)
11519795 .. 11521642
1848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1064700.v1.1.491

Sequence Viewer

Length: 894 bp
ATGAATGATCTAATGACCAAGTCATTCCTCAGTTACGTGGATCTCAAGAAGCAGGCTATGAAAGATCTCGAGTCCCAACCTGACCTTGAAATGGGGAAACTTGACGCCGCTGACGAACAGAACCTCGCCGGATTTTTCGAACAAGTGAACGCAATGAAGGCTGACATGGAAGAGATCACAAACCTCCTTGTTGATCTTCAGGACCTGAATGAAGAAACCAAGTCCACTCACAGTGCTAAAGTGCTCAGAGGGTTGAGAGACAGAATCAATGCTGATATGGTTACTATTCTGAGGAAAGCGAAAAGCATCAAAGCGAGTTTGGAGTCGCTTGATCGTTGTAACGTGGCTAATCGAAGTGTGTCTGCGGCCTACAAGGAAGGAAGCCCTGTTGATCGAATGAGGATTTCGGTGACGAATGGTTTGAGAATTAAGCTGAGAGAGATTATGAATGATTTTCAGATCTTGAGAGAACAGATTGTTAAGGAGCACAAAGACGGTCTCAAGAGGAGGTATTTCAGTGCCACTGGGGAGGAAGCGAGTGATGAAGTGATTGATAAGATGATTTCGACAAATGGGCAGGTCAAAACTTTCGAAGGAAAGGCGGAGCTGGTGATGGAGAATCAGGAAAGGCATGAGGTCTTGAAGGACATACAGAGAAGCTTGACGGAGCTCCATCAAGTGTTCCTTGACATGGCTGTGTTGGTTGAAAAGCAAGGCGAACAGTTGGATGACATCGAACAGAATGTGGCTGGTGCTGGGGCTTATATTAATGATGGAACTAATGCGCTTTCTGATGCTAAGAAGATGAAAAAGAGAAGAGGAAAATGGGTTTGTTGGATTGGCGCTATTGTGTTGTTTATGTTGCTTGTGTGTCTGGTTTCCATCTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 ko04721 map04130 map04721
Pfam Domains
Protein Families
SNARE Syntaxin

Protein Analysis

298

Amino Acids

33.59

Weight (kDa)

5.61

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 39 - 197 8.6e-44 Syntaxin
SNARE PF05739 240 - 290 5.1e-08 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 568
AciI CCGC 3 cut(s) 108, 365, 602
AclWI GGATC 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 182
AcyI GRCGYC 1 cut(s) 105
AfiI CCNNNNNNNGG 2 cut(s) 91, 691
AgsI TTSAA 3 cut(s) 89, 643, 707
AluBI AGCT 4 cut(s) 433, 607, 660, 670
AluI AGCT 4 cut(s) 433, 607, 660, 670
Alw21I GWGCWC 3 cut(s) 246, 489, 672
Alw26I GTCTC 2 cut(s) 252, 503
AlwI GGATC 1 cut(s) 48
AlwNI CAGNNNCTG 1 cut(s) 205
Ama87I CYCGRG 1 cut(s) 68
AoxI GGCC 1 cut(s) 366
ArsI GACNNNNNNTTYG 2 cut(s) 403, 435
AseI ATTAAT 1 cut(s) 768
AspLEI GCGC 2 cut(s) 787, 845
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 2 cut(s) 421, 622
AsuII TTCGAA 2 cut(s) 138, 591
AvaI CYCGRG 1 cut(s) 68
AvaII GGWCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 672
Bbv12I GWGCWC 3 cut(s) 246, 489, 672
BccI CCATC 4 cut(s) 607, 681, 767, 890
BcoDI GTCTC 2 cut(s) 252, 503
BfoI RGCGCY 1 cut(s) 846
BfuAI ACCTGC 1 cut(s) 568
BglII AGATCT 2 cut(s) 64, 459
BisI GCNGC 2 cut(s) 108, 366
BlsI GCNGC 2 cut(s) 109, 367
Bme18I GGWCC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 202
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 2 cut(s) 315, 784
BmuI ACTGGG 1 cut(s) 534
Bpu14I TTCGAA 2 cut(s) 138, 591
BpuEI CTTGAG 3 cut(s) 29, 484, 485
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 503
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 691
Bse1I ACTGG 1 cut(s) 529
Bse3DI GCAATG 1 cut(s) 159
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 2 cut(s) 91, 691
BseMI GCAATG 1 cut(s) 159
BseMII CTCAG 4 cut(s) 43, 259, 281, 425
BseNI ACTGG 1 cut(s) 529
BseRI GAGGAG 1 cut(s) 520
BseYI CCCAGC 1 cut(s) 755
BshFI GGCC 1 cut(s) 368
BsiHKAI GWGCWC 3 cut(s) 246, 489, 672
BsiHKCI CYCGRG 1 cut(s) 68
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 2 cut(s) 91, 691
BsmAI GTCTC 2 cut(s) 252, 503
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 1 cut(s) 68
Bsp119I TTCGAA 2 cut(s) 138, 591
Bsp1286I GDGCHC 3 cut(s) 246, 489, 672
Bsp143I GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
BspACI CCGC 3 cut(s) 108, 365, 602
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 4 cut(s) 42, 258, 282, 426
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 1 cut(s) 48
BspT104I TTCGAA 2 cut(s) 138, 591
BspTNI GGTCTC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 159
BsrI ACTGG 1 cut(s) 529
BssMI GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
BssNI GRCGYC 1 cut(s) 105
Bst4CI ACNGT 3 cut(s) 233, 497, 723
Bst6I CTCTTC 2 cut(s) 165, 811
BstACI GRCGYC 1 cut(s) 105
BstBAI YACGTR 1 cut(s) 37
BstBI TTCGAA 2 cut(s) 138, 591
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 5 cut(s) 29, 245, 290, 434, 798
BstF5I GGATG 1 cut(s) 733
BstH2I RGCGCY 1 cut(s) 846
BstHHI GCGC 2 cut(s) 787, 845
BstKTI GATC 8 cut(s) 10, 43, 67, 177, 196, 334, 394, 462
BstMAI GTCTC 2 cut(s) 252, 503
BstMBI GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
BstMWI GCNNNNNNNGC 1 cut(s) 158
BstX2I RGATCY 3 cut(s) 40, 64, 459
BstYI RGATCY 3 cut(s) 40, 64, 459
BsuRI GGCC 1 cut(s) 368
BtsCI GGATG 1 cut(s) 733
BtsIMutI CAGTG 3 cut(s) 238, 522, 523
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 1 cut(s) 54
CaiI CAGNNNCTG 1 cut(s) 205
CfoI GCGC 2 cut(s) 787, 845
Cfr13I GGNCC 1 cut(s) 202
CseI GACGC 1 cut(s) 113
CviAII CATG 3 cut(s) 166, 632, 691
DdeI CTNAG 5 cut(s) 29, 245, 290, 434, 798
DpnI GATC 8 cut(s) 9, 42, 66, 176, 195, 333, 393, 461
DpnII GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
Eam1104I CTCTTC 2 cut(s) 165, 811
EarI CTCTTC 2 cut(s) 165, 811
EciI GGCGGA 1 cut(s) 617
Ecl136II GAGCTC 1 cut(s) 670
Eco24I GRGCYC 1 cut(s) 672
Eco31I GGTCTC 1 cut(s) 503
Eco47I GGWCC 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 670
Eco57I CTGAAG 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 68
EcoICRI GAGCTC 1 cut(s) 670
EcoO109I RGGNCCY 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 672
FaeI CATG 3 cut(s) 169, 635, 694
FaiI YATR 9 cut(s) 59, 167, 278, 446, 633, 650, 692, 765, 860
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 1 cut(s) 58
FatI CATG 3 cut(s) 165, 631, 690
Fnu4HI GCNGC 2 cut(s) 108, 366
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 2 cut(s) 108, 366
GlaI GCGC 2 cut(s) 786, 844
GluI GCNGC 2 cut(s) 108, 366
GsaI CCCAGC 1 cut(s) 759
HaeII RGCGCY 1 cut(s) 846
HaeIII GGCC 1 cut(s) 368
HapII CCGG 1 cut(s) 129
HgaI GACGC 1 cut(s) 113
HhaI GCGC 2 cut(s) 787, 845
Hin1I GRCGYC 1 cut(s) 105
Hin1II CATG 3 cut(s) 169, 635, 694
Hin6I GCGC 2 cut(s) 785, 843
HinP1I GCGC 2 cut(s) 785, 843
HindIII AAGCTT 1 cut(s) 658
HinfI GANTC 4 cut(s) 71, 264, 323, 619
HpaII CCGG 1 cut(s) 129
HphI GGTGA 2 cut(s) 421, 622
Hpy166II GTNNAC 2 cut(s) 148, 225
Hpy188I TCNGA 4 cut(s) 248, 291, 459, 793
Hpy188III TCNNGA 7 cut(s) 46, 68, 200, 463, 502, 623, 640
Hpy8I GTNNAC 2 cut(s) 148, 225
HpyAV CCTTC 4 cut(s) 151, 371, 587, 637
HpyCH4III ACNGT 3 cut(s) 233, 497, 723
HpyCH4IV ACGT 2 cut(s) 36, 342
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 5 cut(s) 29, 245, 290, 434, 798
HpySE526I ACGT 2 cut(s) 36, 342
Hsp92I GRCGYC 1 cut(s) 105
Hsp92II CATG 3 cut(s) 169, 635, 694
HspAI GCGC 2 cut(s) 785, 843
Kzo9I GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
LmnI GCTCC 4 cut(s) 484, 604, 667, 675
LweI GCATC 2 cut(s) 315, 784
MaeII ACGT 2 cut(s) 36, 342
MaeIII GTNAC 4 cut(s) 32, 280, 338, 409
MalI GATC 8 cut(s) 9, 42, 66, 176, 195, 333, 393, 461
MboI GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
MboII GAAGA 5 cut(s) 182, 188, 224, 814, 828
MflI RGATCY 3 cut(s) 40, 64, 459
MhlI GDGCHC 3 cut(s) 246, 489, 672
MluCI AATT 1 cut(s) 426
MlyI GAGTC 2 cut(s) 80, 332
MmeI TCCRAC 2 cut(s) 705, 815
MseI TTAA 4 cut(s) 429, 480, 768, 892
MslI CAYNNNNRTG 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 129
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
NlaIII CATG 3 cut(s) 169, 635, 694
NmuCI GTSAC 1 cut(s) 409
NspV TTCGAA 2 cut(s) 138, 591
PaeR7I CTCGAG 1 cut(s) 68
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 2 cut(s) 264, 619
PflFI GACNNNGTC 1 cut(s) 19
PkrI GCNGC 2 cut(s) 109, 367
PleI GAGTC 2 cut(s) 79, 331
PpsI GAGTC 2 cut(s) 79, 331
Ppu21I YACGTR 1 cut(s) 37
PpuMI RGGWCCY 1 cut(s) 202
PshBI ATTAAT 1 cut(s) 768
Psp124BI GAGCTC 1 cut(s) 672
Psp5II RGGWCCY 1 cut(s) 202
PspFI CCCAGC 1 cut(s) 755
PspPI GGNCC 1 cut(s) 202
PspPPI RGGWCCY 1 cut(s) 202
PstNI CAGNNNCTG 1 cut(s) 205
PsuI RGATCY 3 cut(s) 40, 64, 459
PsyI GACNNNGTC 1 cut(s) 19
RseI CAYNNNNRTG 1 cut(s) 695
SacI GAGCTC 1 cut(s) 672
SaqAI TTAA 4 cut(s) 429, 480, 768, 892
SatI GCNGC 2 cut(s) 108, 366
Sau3AI GATC 8 cut(s) 7, 40, 64, 174, 193, 331, 391, 459
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 2 cut(s) 80, 332
SduI GDGCHC 3 cut(s) 246, 489, 672
SfaNI GCATC 2 cut(s) 315, 784
Sfr274I CTCGAG 1 cut(s) 68
SfuI TTCGAA 2 cut(s) 138, 591
SinI GGWCC 1 cut(s) 202
SlaI CTCGAG 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 4 cut(s) 44, 68, 463, 500
SmoI CTYRAG 4 cut(s) 44, 68, 463, 500
Sse9I AATT 1 cut(s) 426
SsiI CCGC 3 cut(s) 108, 365, 602
SstI GAGCTC 1 cut(s) 672
TaaI ACNGT 3 cut(s) 233, 497, 723
TaiI ACGT 2 cut(s) 39, 345
TaqI TCGA 7 cut(s) 69, 138, 352, 394, 566, 591, 735
TasI AATT 1 cut(s) 426
TauI GCSGC 2 cut(s) 110, 368
TfiI GAWTC 2 cut(s) 264, 619
Tru1I TTAA 4 cut(s) 429, 480, 768, 892
Tru9I TTAA 4 cut(s) 429, 480, 768, 892
TscAI CASTG 3 cut(s) 238, 523, 529
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 7 cut(s) 17, 74, 170, 225, 461, 558, 821
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 3 cut(s) 238, 523, 529
Tth111I GACNNNGTC 1 cut(s) 19
VpaK11BI GGWCC 1 cut(s) 202
VspI ATTAAT 1 cut(s) 768
XhoI CTCGAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.