MD02G1180700.v1.1

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003836
Physical Location & Seq
Forward (+)
16204790 .. 16212844
8055 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1180700.v1.1.491

Sequence Viewer

Length: 2514 bp
ATGGTGAATAAAGGTGTGATGGAGATATCATTGTTCAAAGCACTTCTTAATAACATCTCCTCTTTTTTCCATTTATCATCTCATGACAACATAAACTTTGACCCGGTTTTGAAGTACTACAAAAGAGCTGAAGAGATATTAAAATTGTTGAAGACTGTACTTGATGCCATTGCTGATTCTGAAATAGCTTCTTATGAACTGCTTAATAAGCCATTTGAAGAACTGGGTCAATATGTTGATGAGTTGAGGGAGCAAATTGAAGACTGGCAGCCATTGTTTAGTAAAGTCAATCTTGTTCTTCAGGTTGAGTCATTGATATCAAAGATTTGGACTTCTGGCCTGGATATCTTTCAACTGTTGAAGGTATCCCCGCAGCATCTTCCTGATGAATTGGGTTCAGCATCTCTTGAGCACTGCATACAAAAACTTAAGCACATGGGATATGAACAAATGTCAACTGTCATTAAAGATGCTGTAAACGATCAAGTGGAGGGTGTTGGACCGAGCTCAGAGATCCTAGTGAAAATTGCAGAGGGCCTTAGCTTGAGGTCCAACCAGGAGATTCTTATTGAGGCTGTAGCACTTGAAAAGTTGAAGGAAAATGCTGAACAATCTGAAAAGATTGAGGAAGCAGAGTATATTGAGCATCTGATTTCCCTTGTTACCCGTATGCACGAGCGCCTTATTATGATAAAGCAATCCGAGAGCTGCAGCCCCGTTCCAATACCTGCTGATTTCTGCTGCCCCCTCTCTCTGGAGCTGATGACGGATCCAGTGATCGTGGCATCAGGTCAAACCTACGAGAGGACTTTCATCAAGAACTGGATCGGTCTTGGCCTCACTGTTTGTCCAAAGACACGGCAGACTTTGGCTCACGCCAATTTGATACCTAATTACACTGTGAAGGCACTAATTGCAAATTGGTGTGAGTCAAACAATGTGAAACTGCCTGATCCCACTAAGGCAGCACACCTTCTTGGGCAAGCAGAGCCTGGTGCACCAAAGGATTCATCTATTTTTCCTCATTCCAGGGTCAGTCAATCGATGTCACCTGAGTCAACTCGGTCTACTGGTTCGCCCTCGAAGAACTTGTTCTCATCTGGTCCACTGTATCAGGAGAGAAGTTCTCCATTGCATCCTCGTTCTACATCAGAAGGTTCCTTGCCTGGTATAGTTGGAACTAGGCAGGATTTGGATATTGAAAGAATATCACTAGCAAATTCTGAAGACAGGTCTGCCAACTTAGAAGAAAGGAGTATAGATCTAGATAGCCAACACTCTATGTCACCATCTCGAGATGAACTTCCTAATTCTATTGAAGTTGAGCAGTCATCTCAAAGCCATAATAGAACCGCGTCAGCTTCCAGCGTACTTTCCAATGCCAATTGTAGCCAAGGAACTCCAGTCAATGGCAATGGGGCCTTACAGGTATCGACCAGTCTGTCAGGCTACAGCAGTGATGCTTCTGGGGAGTTGAAATCAGAACCGCAAGCTGTCGCTACCTTGCCCCCCCAACAGAGACAAACTGAGTCGCCAACCAGAATAGTAGAGGCAAGACCTCGAAACCAAATGTGGCGCCGACCATCTAGCTTAATCCCAAGGATAGTTTCTTCTCCACCTATTGAAACAAGACCTGACCTTTCTGAACTTGAAGCCCAAGTTCGTAACCTGGTTGAGGACTTGAAAAGCACTTCACTTGATACACAAAGAGAGGCAACATTTCAATTCCGGTTACTTGCCAAGCATAATATGGATAATCGGATTGTTATTGCAAGTTGTGGGGCCATTGGCCTGTTGGTGGATTTGCTCCACTCTGCAGATACAAGAGTTCAGGAGAATGCTGTCACAGCACTTCTTAACTTATCGATCAATGATAACAACAAAACTGCAATTGCTACTGCGAATGCAATTGAACCTCTGATTCATGTCCTTGAGACGGGGAGTGCAGAGGCCAAGGAGAACTCAGCTGCCACTCTCTTCAGCCTTTCGGTGATCGAGGACAACAAGGTTCGCATTGGAAGGTCAGGGGCTATCAGGCCTTTGGTTGATTTATTGGGGAATGGGAGTCCGAGAGGGAGGAAAGATGCAGCCACAGCTTTGTTTAATTTGTCAATTTTTCATGAGAACAAGGGTCGAATTGTTCAAGCTGGTGCTGTTAAGTACCTAGTGGAGTTGATGGACCCAGCGGCTGGAATGGTTGACAAGGCAGTTGCTGTTTTGGCTAATCTTTCAACAATTCCCGAGGGGAGGACAGCAATTGGTCAGGAGGGTGGGATCCCTGTTCTTGTCGAGGTTGTTGAGTTGGGTTCTGCAAGAGGGAAGGAAAATGCAGCTGCTGCTCTATTACAGCTCTGCACAAACAGTAATCGATATTGCAGTCAGGTTCTCCAAGAAGGAGCTGTCCCACCATTAGTAGTATTGTCACAATCTGGCACCCCAAGGGCCAAAGAAAAGGCGCAGACGCTCCTTAGCTTCTTCAGAAACCATAGACAGGGTAATGCTGGTAGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

838

Amino Acids

91.25

Weight (kDa)

5.55

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 8 - 147 4.7e-62 PUB2-like, N-terminal domain
U-box PF04564 242 - 312 1.4e-21 U-box domain
ARM_PUB PF25598 553 - 829 3.3e-28 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 554 - 726 2.7e-08 ARMC5
Arm PF00514 585 - 622 6.3e-10 Armadillo/beta-catenin-like repeat
Arm PF00514 667 - 704 2.1e-07 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 698 - 823 2.4e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1441
Acc36I ACCTGC 1 cut(s) 736
AccB1I GGYRCC 2 cut(s) 1575, 2432
AccB7I CCANNNNNTGG 2 cut(s) 1409, 2189
AccI GTMKAC 1 cut(s) 1067
AccII CGCG 1 cut(s) 1355
AciI CCGC 4 cut(s) 371, 1353, 1487, 2186
AclWI GGATC 7 cut(s) 508, 764, 777, 833, 947, 2269, 2282
AcsI RAATTY 1 cut(s) 1219
AcuI CTGAAG 5 cut(s) 150, 284, 1245, 1963, 2461
AcyI GRCGYC 1 cut(s) 1576
AfaI GTAC 4 cut(s) 116, 159, 1371, 2162
AfiI CCNNNNNNNGG 9 cut(s) 754, 804, 1409, 1675, 1798, 1936, 2189, 2247, 2491
AflII CTTAAG 1 cut(s) 428
AjnI CCWGG 6 cut(s) 339, 555, 991, 1028, 1165, 1668
AjuI GAANNNNNNNTTGG 2 cut(s) 1591, 1623
Alw21I GWGCWC 3 cut(s) 414, 509, 1000
Alw26I GTCTC 2 cut(s) 1513, 1928
Alw44I GTGCAC 1 cut(s) 996
AlwI GGATC 7 cut(s) 508, 764, 777, 833, 947, 2269, 2282
AlwNI CAGNNNCTG 4 cut(s) 992, 2189, 2213, 2336
Ama87I CYCGRG 2 cut(s) 1293, 2240
AoxI GGCC 9 cut(s) 337, 535, 835, 1419, 1782, 1789, 1950, 2036, 2442
ApaLI GTGCAC 1 cut(s) 996
ApoI RAATTY 1 cut(s) 1219
Asp700I GAANNNNTTC 1 cut(s) 1091
AspLEI GCGC 3 cut(s) 681, 1578, 2458
AspS9I GGNCC 8 cut(s) 500, 535, 549, 1103, 1419, 1782, 2179, 2442
AsuC2I CCSGG 1 cut(s) 104
AsuHPI GGTGA 4 cut(s) 16, 1041, 1278, 2002
AvaI CYCGRG 2 cut(s) 1293, 2240
AvaII GGWCC 4 cut(s) 500, 549, 1103, 2179
BaeGI GKGCMC 1 cut(s) 1000
BaeI ACNNNNGTAYC 2 cut(s) 1812, 1845
BamHI GGATCC 2 cut(s) 769, 2274
BanI GGYRCC 2 cut(s) 1575, 2432
BanII GRGCYC 1 cut(s) 509
BauI CACGAG 1 cut(s) 674
BbsI GAAGAC 3 cut(s) 158, 267, 1233
Bbv12I GWGCWC 3 cut(s) 414, 509, 1000
BccI CCATC 4 cut(s) 13, 1297, 1591, 2170
BceAI ACGGC 1 cut(s) 875
BciT130I CCWGG 6 cut(s) 341, 557, 993, 1030, 1167, 1670
BciVI GTATCC 1 cut(s) 376
BcnI CCSGG 1 cut(s) 104
BcoDI GTCTC 2 cut(s) 1513, 1928
BfaI CTAG 6 cut(s) 518, 1182, 1214, 1265, 1587, 2165
BfmI CTRYAG 4 cut(s) 576, 709, 1450, 1815
BfoI RGCGCY 2 cut(s) 682, 1579
BfrI CTTAAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 736
BfuI GTATCC 1 cut(s) 376
BglII AGATCT 1 cut(s) 1261
BmcAI AGTACT 1 cut(s) 116
Bme1390I CCNGG 7 cut(s) 104, 341, 557, 993, 1030, 1167, 1670
Bme18I GGWCC 4 cut(s) 500, 549, 1103, 2179
BmeT110I CYCGRG 2 cut(s) 1293, 2240
BmgT120I GGNCC 8 cut(s) 500, 535, 549, 1103, 1419, 1782, 2179, 2442
BmiI GGNNCC 8 cut(s) 771, 1159, 1420, 1577, 1783, 2181, 2276, 2434
BmrFI CCNGG 7 cut(s) 104, 341, 557, 993, 1030, 1167, 1670
BmrI ACTGGG 1 cut(s) 233
BmsI GCATC 9 cut(s) 154, 385, 410, 460, 655, 794, 1144, 1450, 2074
BmuI ACTGGG 1 cut(s) 233
BpiI GAAGAC 3 cut(s) 158, 267, 1233
BplI GAGNNNNNCTC 2 cut(s) 1111, 1143
BpmI CTGGAG 2 cut(s) 776, 1386
Bpu10I CCTNAGC 2 cut(s) 539, 2468
BpuEI CTTGAG 3 cut(s) 428, 565, 1952
BpuMI CCSGG 1 cut(s) 104
Bsa29I ATCGAT 3 cut(s) 1043, 1865, 2368
BsaHI GRCGYC 1 cut(s) 1576
BsaJI CCNNGG 6 cut(s) 1029, 1393, 1598, 1953, 2241, 2438
BsaWI WCCGGW 1 cut(s) 1728
Bsc4I CCNNNNNNNGG 9 cut(s) 754, 804, 1409, 1675, 1798, 1936, 2189, 2247, 2491
Bse1I ACTGG 7 cut(s) 228, 269, 773, 827, 1075, 1403, 1437
Bse3DI GCAATG 3 cut(s) 168, 1130, 1420
BseBI CCWGG 6 cut(s) 341, 557, 993, 1030, 1167, 1670
BseCI ATCGAT 3 cut(s) 1043, 1865, 2368
BseDI CCNNGG 6 cut(s) 1029, 1393, 1598, 1953, 2241, 2438
BseGI GGATG 1 cut(s) 1135
BseLI CCNNNNNNNGG 9 cut(s) 754, 804, 1409, 1675, 1798, 1936, 2189, 2247, 2491
BseMI GCAATG 3 cut(s) 168, 1130, 1420
BseMII CTCAG 4 cut(s) 522, 1044, 1518, 1977
BseNI ACTGG 7 cut(s) 228, 269, 773, 827, 1075, 1403, 1437
BseRI GAGGAG 1 cut(s) 49
BseSI GKGCMC 1 cut(s) 1000
BseYI CCCAGC 1 cut(s) 2182
BsgI GTGCAG 2 cut(s) 1965, 2338
Bsh1236I CGCG 1 cut(s) 1355
BshFI GGCC 9 cut(s) 339, 537, 837, 1421, 1784, 1791, 1952, 2038, 2444
BshNI GGYRCC 2 cut(s) 1575, 2432
BshVI ATCGAT 3 cut(s) 1043, 1865, 2368
BsiHKAI GWGCWC 3 cut(s) 414, 509, 1000
BsiHKCI CYCGRG 2 cut(s) 1293, 2240
BsiSI CCGG 2 cut(s) 104, 1729
BslFI GGGAC 1 cut(s) 2387
BslI CCNNNNNNNGG 9 cut(s) 754, 804, 1409, 1675, 1798, 1936, 2189, 2247, 2491
BsmAI GTCTC 2 cut(s) 1513, 1928
BsmBI CGTCTC 1 cut(s) 1928
BsmFI GGGAC 1 cut(s) 2387
BsmI GAATGC 2 cut(s) 1843, 1909
BsnI GGCC 9 cut(s) 339, 537, 837, 1421, 1784, 1791, 1952, 2038, 2444
BsoBI CYCGRG 2 cut(s) 1293, 2240
Bsp1286I GDGCHC 3 cut(s) 414, 509, 1000
BspACI CCGC 4 cut(s) 371, 1353, 1487, 2186
BspANI GGCC 9 cut(s) 339, 537, 837, 1421, 1784, 1791, 1952, 2038, 2444
BspCNI CTCAG 4 cut(s) 521, 1045, 1519, 1976
BspDI ATCGAT 3 cut(s) 1043, 1865, 2368
BspFNI CGCG 1 cut(s) 1355
BspHI TCATGA 2 cut(s) 82, 2119
BspLI GGNNCC 8 cut(s) 771, 1159, 1420, 1577, 1783, 2181, 2276, 2434
BspMAI CTGCAG 2 cut(s) 713, 1819
BspMI ACCTGC 1 cut(s) 736
BspPI GGATC 7 cut(s) 508, 764, 777, 833, 947, 2269, 2282
BspT107I GGYRCC 2 cut(s) 1575, 2432
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 3 cut(s) 168, 1130, 1420
BsrI ACTGG 7 cut(s) 228, 269, 773, 827, 1075, 1403, 1437
BssECI CCNNGG 6 cut(s) 1029, 1393, 1598, 1953, 2241, 2438
BssNI GRCGYC 1 cut(s) 1576
BssSI CACGAG 1 cut(s) 674
BssT1I CCWWGG 4 cut(s) 1393, 1598, 1953, 2438
Bst2BI CACGAG 1 cut(s) 674
Bst2UI CCWGG 6 cut(s) 341, 557, 993, 1030, 1167, 1670
Bst4CI ACNGT 7 cut(s) 157, 357, 460, 844, 901, 1110, 2363
Bst6I CTCTTC 2 cut(s) 126, 1982
BstACI GRCGYC 1 cut(s) 1576
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 2 cut(s) 914, 2336
BstC8I GCNNGC 2 cut(s) 984, 1491
BstDEI CTNAG 8 cut(s) 508, 539, 960, 1053, 1243, 1527, 1963, 2468
BstENI CCTNNNNNAGG 2 cut(s) 802, 1673
BstF5I GGATG 1 cut(s) 1135
BstFNI CGCG 1 cut(s) 1355
BstH2I RGCGCY 2 cut(s) 682, 1579
BstHHI GCGC 3 cut(s) 681, 1578, 2458
BstMAI GTCTC 2 cut(s) 1513, 1928
BstMWI GCNNNNNNNGC 7 cut(s) 208, 914, 988, 1847, 2093, 2219, 2336
BstNI CCWGG 6 cut(s) 341, 557, 993, 1030, 1167, 1670
BstSCI CCNGG 7 cut(s) 102, 339, 555, 991, 1028, 1165, 1668
BstSFI CTRYAG 4 cut(s) 576, 709, 1450, 1815
BstSLI GKGCMC 1 cut(s) 1000
BstUI CGCG 1 cut(s) 1355
BstV2I GAAGAC 3 cut(s) 158, 267, 1233
BstX2I RGATCY 4 cut(s) 513, 769, 1261, 2274
BstYI RGATCY 4 cut(s) 513, 769, 1261, 2274
Bsu15I ATCGAT 3 cut(s) 1043, 1865, 2368
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 9 cut(s) 339, 537, 837, 1421, 1784, 1791, 1952, 2038, 2444
BsuTUI ATCGAT 3 cut(s) 1043, 1865, 2368
BtsCI GGATG 1 cut(s) 1135
BtsI GCAGTG 2 cut(s) 412, 1462
BtsIMutI CAGTG 6 cut(s) 412, 780, 840, 897, 1106, 1462
BveI ACCTGC 1 cut(s) 736
Cac8I GCNNGC 2 cut(s) 984, 1491
CaiI CAGNNNCTG 4 cut(s) 992, 2189, 2213, 2336
CciI TCATGA 2 cut(s) 82, 2119
CfoI GCGC 3 cut(s) 681, 1578, 2458
Cfr13I GGNCC 8 cut(s) 500, 535, 549, 1103, 1419, 1782, 2179, 2442
ClaI ATCGAT 3 cut(s) 1043, 1865, 2368
CseI GACGC 2 cut(s) 1344, 2470
CsiI ACCWGGT 1 cut(s) 1668
Csp6I GTAC 4 cut(s) 115, 158, 1370, 2161
CviAII CATG 4 cut(s) 83, 436, 1925, 2120
CviQI GTAC 4 cut(s) 115, 158, 1370, 2161
DdeI CTNAG 8 cut(s) 508, 539, 960, 1053, 1243, 1527, 1963, 2468
DinI GGCGCC 1 cut(s) 1577
DrdI GACNNNNNNGTC 1 cut(s) 1441
DseDI GACNNNNNNGTC 1 cut(s) 1441
Eam1104I CTCTTC 2 cut(s) 126, 1982
EarI CTCTTC 2 cut(s) 126, 1982
Ecl136II GAGCTC 1 cut(s) 507
Eco130I CCWWGG 4 cut(s) 1393, 1598, 1953, 2438
Eco147I AGGCCT 1 cut(s) 2038
Eco24I GRGCYC 1 cut(s) 509
Eco32I GATATC 3 cut(s) 27, 318, 346
Eco47I GGWCC 4 cut(s) 500, 549, 1103, 2179
Eco53kI GAGCTC 1 cut(s) 507
Eco57I CTGAAG 5 cut(s) 150, 284, 1245, 1963, 2461
Eco88I CYCGRG 2 cut(s) 1293, 2240
EcoICRI GAGCTC 1 cut(s) 507
EcoNI CCTNNNNNAGG 2 cut(s) 802, 1673
EcoO109I RGGNCCY 2 cut(s) 535, 1419
EcoRII CCWGG 6 cut(s) 339, 555, 991, 1028, 1165, 1668
EcoRV GATATC 3 cut(s) 27, 318, 346
EcoT14I CCWWGG 4 cut(s) 1393, 1598, 1953, 2438
EcoT38I GRGCYC 1 cut(s) 509
EgeI GGCGCC 1 cut(s) 1577
EheI GGCGCC 1 cut(s) 1577
ErhI CCWWGG 4 cut(s) 1393, 1598, 1953, 2438
Esp3I CGTCTC 1 cut(s) 1928
FaeI CATG 4 cut(s) 86, 439, 1928, 2123
FalI AAGNNNNNCTT 4 cut(s) 30, 62, 276, 308
FaqI GGGAC 1 cut(s) 2387
FatI CATG 4 cut(s) 82, 435, 1924, 2119
FauI CCCGC 1 cut(s) 378
FblI GTMKAC 1 cut(s) 1067
FokI GGATG 1 cut(s) 1122
FriOI GRGCYC 1 cut(s) 509
FspBI CTAG 6 cut(s) 518, 1182, 1214, 1265, 1587, 2165
GlaI GCGC 3 cut(s) 680, 1577, 2457
GsaI CCCAGC 1 cut(s) 2186
GsuI CTGGAG 2 cut(s) 776, 1386
HaeII RGCGCY 2 cut(s) 682, 1579
HaeIII GGCC 9 cut(s) 339, 537, 837, 1421, 1784, 1791, 1952, 2038, 2444
HapII CCGG 2 cut(s) 104, 1729
HgaI GACGC 2 cut(s) 1344, 2470
HhaI GCGC 3 cut(s) 681, 1578, 2458
Hin1I GRCGYC 1 cut(s) 1576
Hin1II CATG 4 cut(s) 86, 439, 1928, 2123
Hin6I GCGC 3 cut(s) 679, 1576, 2456
HinP1I GCGC 3 cut(s) 679, 1576, 2456
HincII GTYRAC 3 cut(s) 456, 1059, 2200
HindII GTYRAC 3 cut(s) 456, 1059, 2200
HinfI GANTC 9 cut(s) 176, 308, 562, 929, 1007, 1055, 1529, 1921, 2065
HpaII CCGG 2 cut(s) 104, 1729
HphI GGTGA 4 cut(s) 16, 1041, 1278, 2002
Hpy166II GTNNAC 7 cut(s) 456, 478, 998, 1059, 1068, 1106, 2200
Hpy8I GTNNAC 7 cut(s) 456, 478, 998, 1059, 1068, 1106, 2200
HpyAV CCTTC 8 cut(s) 355, 589, 898, 983, 1148, 2013, 2314, 2387
HpyCH4III ACNGT 7 cut(s) 157, 357, 460, 844, 901, 1110, 2363
HpyF10VI GCNNNNNNNGC 7 cut(s) 208, 914, 988, 1847, 2093, 2219, 2336
HpyF3I CTNAG 8 cut(s) 508, 539, 960, 1053, 1243, 1527, 1963, 2468
Hsp92I GRCGYC 1 cut(s) 1576
Hsp92II CATG 4 cut(s) 86, 439, 1928, 2123
HspAI GCGC 3 cut(s) 679, 1576, 2456
KasI GGCGCC 1 cut(s) 1575
LmnI GCTCC 5 cut(s) 250, 757, 1812, 2396, 2469
LweI GCATC 9 cut(s) 154, 385, 410, 460, 655, 794, 1144, 1450, 2074
MabI ACCWGGT 1 cut(s) 1668
MaeI CTAG 6 cut(s) 518, 1182, 1214, 1265, 1587, 2165
MaeIII GTNAC 7 cut(s) 661, 1047, 1284, 1664, 1731, 1843, 2421
MfeI CAATTG 4 cut(s) 1384, 1890, 1908, 2256
MflI RGATCY 4 cut(s) 513, 769, 1261, 2274
MhlI GDGCHC 3 cut(s) 414, 509, 1000
Mly113I GGCGCC 1 cut(s) 1576
MlyI GAGTC 5 cut(s) 317, 938, 1064, 1538, 2074
MmeI TCCRAC 3 cut(s) 478, 576, 1156
MroXI GAANNNNTTC 1 cut(s) 1091
MseI TTAA 9 cut(s) 48, 140, 204, 429, 465, 1592, 1857, 2103, 2157
MspA1I CMGCKG 3 cut(s) 1967, 2186, 2333
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 104, 1729
MspR9I CCNGG 7 cut(s) 104, 341, 557, 993, 1030, 1167, 1670
MunI CAATTG 4 cut(s) 1384, 1890, 1908, 2256
Mva1269I GAATGC 2 cut(s) 1843, 1909
MvaI CCWGG 6 cut(s) 341, 557, 993, 1030, 1167, 1670
MvnI CGCG 1 cut(s) 1355
MwoI GCNNNNNNNGC 7 cut(s) 208, 914, 988, 1847, 2093, 2219, 2336
NarI GGCGCC 1 cut(s) 1576
NciI CCSGG 1 cut(s) 104
NlaIII CATG 4 cut(s) 86, 439, 1928, 2123
NlaIV GGNNCC 8 cut(s) 771, 1159, 1420, 1577, 1783, 2181, 2276, 2434
NmuCI GTSAC 4 cut(s) 1047, 1284, 1843, 2421
PaeR7I CTCGAG 1 cut(s) 1293
PagI TCATGA 2 cut(s) 82, 2119
PceI AGGCCT 1 cut(s) 2038
PctI GAATGC 2 cut(s) 1843, 1909
PdmI GAANNNNTTC 1 cut(s) 1091
PfeI GAWTC 4 cut(s) 176, 562, 1007, 1921
PflMI CCANNNNNTGG 2 cut(s) 1409, 2189
PleI GAGTC 5 cut(s) 316, 937, 1063, 1537, 2073
PluTI GGCGCC 1 cut(s) 1579
PpsI GAGTC 5 cut(s) 316, 937, 1063, 1537, 2073
Psp124BI GAGCTC 1 cut(s) 509
Psp6I CCWGG 6 cut(s) 339, 555, 991, 1028, 1165, 1668
PspFI CCCAGC 1 cut(s) 2182
PspGI CCWGG 6 cut(s) 339, 555, 991, 1028, 1165, 1668
PspN4I GGNNCC 8 cut(s) 771, 1159, 1420, 1577, 1783, 2181, 2276, 2434
PspPI GGNCC 8 cut(s) 500, 535, 549, 1103, 1419, 1782, 2179, 2442
PstI CTGCAG 2 cut(s) 713, 1819
PstNI CAGNNNCTG 4 cut(s) 992, 2189, 2213, 2336
PsuI RGATCY 4 cut(s) 513, 769, 1261, 2274
PvuII CAGCTG 2 cut(s) 1967, 2333
RsaI GTAC 4 cut(s) 116, 159, 1371, 2162
RsaNI GTAC 4 cut(s) 115, 158, 1370, 2161
SacI GAGCTC 1 cut(s) 509
SaqAI TTAA 9 cut(s) 48, 140, 204, 429, 465, 1592, 1857, 2103, 2157
Sau96I GGNCC 8 cut(s) 500, 535, 549, 1103, 1419, 1782, 2179, 2442
ScaI AGTACT 1 cut(s) 116
SchI GAGTC 5 cut(s) 317, 938, 1064, 1538, 2074
ScrFI CCNGG 7 cut(s) 104, 341, 557, 993, 1030, 1167, 1670
SduI GDGCHC 3 cut(s) 414, 509, 1000
SexAI ACCWGGT 1 cut(s) 1668
SfaNI GCATC 9 cut(s) 154, 385, 410, 460, 655, 794, 1144, 1450, 2074
SfcI CTRYAG 4 cut(s) 576, 709, 1450, 1815
SfoI GGCGCC 1 cut(s) 1577
Sfr274I CTCGAG 1 cut(s) 1293
SinI GGWCC 4 cut(s) 500, 549, 1103, 2179
SlaI CTCGAG 1 cut(s) 1293
SmlI CTYRAG 5 cut(s) 407, 428, 544, 1293, 1931
SmoI CTYRAG 5 cut(s) 407, 428, 544, 1293, 1931
SseBI AGGCCT 1 cut(s) 2038
SsiI CCGC 4 cut(s) 371, 1353, 1487, 2186
SspDI GGCGCC 1 cut(s) 1575
SspMI CTAG 6 cut(s) 518, 1182, 1214, 1265, 1587, 2165
SstI GAGCTC 1 cut(s) 509
StuI AGGCCT 1 cut(s) 2038
StyD4I CCNGG 7 cut(s) 102, 339, 555, 991, 1028, 1165, 1668
StyI CCWWGG 4 cut(s) 1393, 1598, 1953, 2438
TaaI ACNGT 7 cut(s) 157, 357, 460, 844, 901, 1110, 2363
TaqII GACCGA 3 cut(s) 517, 818, 1053
TatI WGTACW 2 cut(s) 114, 157
TauI GCSGC 1 cut(s) 2189
TfiI GAWTC 4 cut(s) 176, 562, 1007, 1921
Tru1I TTAA 9 cut(s) 48, 140, 204, 429, 465, 1592, 1857, 2103, 2157
Tru9I TTAA 9 cut(s) 48, 140, 204, 429, 465, 1592, 1857, 2103, 2157
TscAI CASTG 6 cut(s) 419, 780, 847, 904, 1113, 1462
TseFI GTSAC 4 cut(s) 1047, 1284, 1843, 2421
Tsp45I GTSAC 4 cut(s) 1047, 1284, 1843, 2421
TspDTI ATGAA 8 cut(s) 210, 402, 459, 802, 999, 1314, 1913, 2108
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 6 cut(s) 419, 780, 847, 904, 1113, 1462
Van91I CCANNNNNTGG 2 cut(s) 1409, 2189
Vha464I CTTAAG 1 cut(s) 428
VneI GTGCAC 1 cut(s) 996
VpaK11BI GGWCC 4 cut(s) 500, 549, 1103, 2179
XagI CCTNNNNNAGG 2 cut(s) 802, 1673
XapI RAATTY 1 cut(s) 1219
XbaI TCTAGA 1 cut(s) 1264
XcmI CCANNNNNNNNNTGG 2 cut(s) 1747, 1792
XhoI CTCGAG 1 cut(s) 1293
XmiI GTMKAC 1 cut(s) 1067
XmnI GAANNNNTTC 1 cut(s) 1091
XspI CTAG 6 cut(s) 518, 1182, 1214, 1265, 1587, 2165
ZrmI AGTACT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.