Rorug02G0140900

RING-type E3 ubiquitin transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003836
Physical Location & Seq
Reverse (-)
12398262 .. 12400181
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0140900.1

Sequence Viewer

Length: 1134 bp
ATGGCGGATTCCTTACCCGAAGACGTGATAGTTCGAATCCTGGAGAGGTTGCCGATCAAATCCCTGATCCGCTTCACCTGCGTTTCGAAACGCTGGCGTTTCATCATACTCTGTGACCCAGAATTCGCCAAGGCCCAATTCCAAATAGCTTCTCACCACCAAACCCTCCGTCACAGGCTCCTCATCTCCACCGCCTGTGAATCTGAATTCAACTCACTAGACTTGGATGCGCCGCCGTCCCCGTCGGGAGACAATTCTTCAGTTAGGTGCCCATTCACGCAACCGGGGGGCCATCCTGTCAGACTACTGTGCTCCTGCAAAGGTTTGATACTTGCACTTGTTTGTAATCAAGAAAACGACATGTATATCTGGAACCCATCAAGTCAATTCTTCAAAAAATTACCTGACCCAGCTGATCTTCCCTCAAAAACTTGTCTGCCCTGCTATGGTTTTGGCTATTTGTCGGCCACTGACGACTACAAAGTTATTGCCAGCTTCAAAGTCAGTGTTGCTGATGATGAGATGGAGGAGAATGGCAGCAGCAAGGAAATGCACATCTTCTCATCAAAAGCCAACACTTGGAAAAGGACTGAAGCCCCTCTCGACTTCGACTTCGACTTCTACTCCGACTCTGATTTTGGGGGGACTCTTTCAAATGAGGCACTTCATTGGCTGGATGATGAGGACGACGGACCTGGCATCTTTGCTTTTGATCTAGCAAAGGAGGAGTTCCAAAGAATGCCGCTGCCTATTCTACTAGATGGTGAGGTTTCGTATCTCAGGGCTTTCCGAGACTCTCTGTGTGTGTTTGATTTTGAAAATCTTGTTCCTGCTAGCTCCATTGATTTGTGGGTGATGCAAAAATATGGCGTGGCTGACTCATGGACCAACCTCTTTAACTTCAAGGTTTCCAATCAGCTTGGGGACATATATTATTGTTTGCCAGTCTCGGTAATGGAAACTAGTACATTTCTGCACAAAGTGACTAGATTCCGTGATGGGAATTTTATAGATTTGATAAGGATTGGTCATACGGAACAGAAGCCTGAAATGCATATGTTGAGCAGGAATACAGGTCTCATGGAAATGATTGAATATGAAGAGACTCTACTTTGGCTTGGTTGTGACTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Arm U-box

Protein Analysis

377

Amino Acids

43.02

Weight (kDa)

4.75

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 3 - 39 3.5e-08 F-box-like
F-box PF00646 4 - 36 1e-09 F-box domain
FBA_3 PF08268 86 - 297 1e-09 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 98 - 251 2.6e-09 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 101 - 310 1e-19 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 267
AccB7I CCANNNNNTGG 1 cut(s) 579
AciI CCGC 5 cut(s) 5, 70, 192, 233, 743
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 3 cut(s) 122, 206, 1003
AcuI CTGAAG 2 cut(s) 243, 612
AdeI CACNNNGTG 1 cut(s) 982
AfaI GTAC 1 cut(s) 967
AfiI CCNNNNNNNGG 2 cut(s) 446, 579
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 7 cut(s) 211, 394, 499, 654, 818, 904, 1094
AhlI ACTAGT 1 cut(s) 962
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 2 cut(s) 39, 694
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 5 cut(s) 149, 413, 495, 837, 919
AluI AGCT 5 cut(s) 149, 413, 495, 837, 919
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 5 cut(s) 243, 786, 952, 1082, 1097
AlwI GGATC 1 cut(s) 61
AoxI GGCC 3 cut(s) 132, 289, 465
ApeKI GCWGC 3 cut(s) 537, 540, 745
ApoI RAATTY 3 cut(s) 122, 206, 1003
ArsI GACNNNNNNTTYG 8 cut(s) 107, 139, 154, 186, 596, 620, 628, 652
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 4 cut(s) 133, 289, 692, 885
AsuC2I CCSGG 1 cut(s) 285
AsuHPI GGTGA 4 cut(s) 67, 146, 776, 865
AsuII TTCGAA 2 cut(s) 34, 86
AsuNHI GCTAGC 1 cut(s) 833
AvaII GGWCC 2 cut(s) 692, 885
BaeGI GKGCMC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 267
BarI GAAGNNNNNNTAC 2 cut(s) 1092, 1124
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 3 cut(s) 549, 552, 732
BccI CCATC 5 cut(s) 300, 385, 517, 755, 992
BceAI ACGGC 1 cut(s) 220
BciT130I CCWGG 2 cut(s) 41, 696
BcnI CCSGG 1 cut(s) 285
BcoDI GTCTC 5 cut(s) 243, 786, 952, 1082, 1097
BcuI ACTAGT 1 cut(s) 962
BfaI CTAG 6 cut(s) 218, 716, 758, 834, 963, 987
BfuAI ACCTGC 1 cut(s) 86
BisI GCNGC 5 cut(s) 233, 538, 541, 743, 746
BlsI GCNGC 5 cut(s) 234, 539, 542, 744, 747
Bme1390I CCNGG 3 cut(s) 41, 285, 696
Bme18I GGWCC 2 cut(s) 692, 885
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 4 cut(s) 133, 289, 692, 885
BmiI GGNNCC 4 cut(s) 179, 269, 290, 374
BmrFI CCNGG 3 cut(s) 41, 285, 696
BmsI GCATC 3 cut(s) 217, 708, 846
BmtI GCTAGC 1 cut(s) 837
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 62
Bpu14I TTCGAA 2 cut(s) 34, 86
BpuMI CCSGG 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 1082
BsaJI CCNNGG 2 cut(s) 129, 284
BsaXI ACNNNNNCTCC 2 cut(s) 608, 638
Bsc4I CCNNNNNNNGG 2 cut(s) 446, 579
Bse1I ACTGG 1 cut(s) 944
BseBI CCWGG 2 cut(s) 41, 696
BseDI CCNNGG 2 cut(s) 129, 284
BseGI GGATG 3 cut(s) 232, 292, 682
BseLI CCNNNNNNNGG 2 cut(s) 446, 579
BseMII CTCAG 1 cut(s) 793
BseNI ACTGG 1 cut(s) 944
BseRI GAGGAG 3 cut(s) 170, 542, 740
BseSI GKGCMC 1 cut(s) 272
BseXI GCAGC 3 cut(s) 549, 552, 732
BseYI CCCAGC 1 cut(s) 409
BsgI GTGCAG 1 cut(s) 959
BshFI GGCC 3 cut(s) 134, 291, 467
BshNI GGYRCC 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 314
BsiSI CCGG 1 cut(s) 284
BslFI GGGAC 3 cut(s) 223, 658, 938
BslI CCNNNNNNNGG 2 cut(s) 446, 579
BsmAI GTCTC 5 cut(s) 243, 786, 952, 1082, 1097
BsmFI GGGAC 3 cut(s) 223, 658, 938
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 3 cut(s) 134, 291, 467
Bso31I GGTCTC 1 cut(s) 1082
Bsp119I TTCGAA 2 cut(s) 34, 86
Bsp1286I GDGCHC 2 cut(s) 272, 314
Bsp143I GATC 4 cut(s) 54, 66, 415, 712
BspACI CCGC 5 cut(s) 5, 70, 192, 233, 743
BspANI GGCC 3 cut(s) 134, 291, 467
BspCNI CTCAG 1 cut(s) 792
BspLI GGNNCC 4 cut(s) 179, 269, 290, 374
BspMI ACCTGC 1 cut(s) 86
BspOI GCTAGC 1 cut(s) 837
BspPI GGATC 1 cut(s) 61
BspT104I TTCGAA 2 cut(s) 34, 86
BspT107I GGYRCC 1 cut(s) 267
BspTNI GGTCTC 1 cut(s) 1082
BsrI ACTGG 1 cut(s) 944
BssECI CCNNGG 2 cut(s) 129, 284
BssMI GATC 4 cut(s) 54, 66, 415, 712
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 2 cut(s) 41, 696
Bst4CI ACNGT 1 cut(s) 309
Bst6I CTCTTC 1 cut(s) 1095
BstBI TTCGAA 2 cut(s) 34, 86
BstC8I GCNNGC 3 cut(s) 95, 493, 835
BstDEI CTNAG 1 cut(s) 779
BstF5I GGATG 3 cut(s) 232, 292, 682
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 4 cut(s) 57, 69, 418, 715
BstMAI GTCTC 5 cut(s) 243, 786, 952, 1082, 1097
BstMBI GATC 4 cut(s) 54, 66, 415, 712
BstMWI GCNNNNNNNGC 2 cut(s) 78, 1051
BstNI CCWGG 2 cut(s) 41, 696
BstNSI RCATGY 1 cut(s) 364
BstSCI CCNGG 3 cut(s) 39, 283, 694
BstSLI GKGCMC 1 cut(s) 272
BstV1I GCAGC 3 cut(s) 549, 552, 732
BstV2I GAAGAC 1 cut(s) 27
BsuRI GGCC 3 cut(s) 134, 291, 467
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 3 cut(s) 232, 292, 682
BtsIMutI CAGTG 2 cut(s) 468, 511
BveI ACCTGC 1 cut(s) 86
Cac8I GCNNGC 3 cut(s) 95, 493, 835
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 4 cut(s) 133, 289, 692, 885
Csp6I GTAC 1 cut(s) 966
CviAII CATG 3 cut(s) 361, 882, 1081
CviQI GTAC 1 cut(s) 966
DdeI CTNAG 1 cut(s) 779
DpnI GATC 4 cut(s) 56, 68, 417, 714
DpnII GATC 4 cut(s) 54, 66, 415, 712
DraIII CACNNNGTG 1 cut(s) 982
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 1 cut(s) 1095
EarI CTCTTC 1 cut(s) 1095
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 1082
Eco47I GGWCC 2 cut(s) 692, 885
Eco57I CTGAAG 2 cut(s) 243, 612
EcoRI GAATTC 2 cut(s) 122, 206
EcoRII CCWGG 2 cut(s) 39, 694
EcoT14I CCWWGG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 1056
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 3 cut(s) 364, 885, 1084
FaqI GGGAC 3 cut(s) 223, 658, 938
FatI CATG 3 cut(s) 360, 881, 1080
FauNDI CATATG 1 cut(s) 1056
Fnu4HI GCNGC 5 cut(s) 233, 538, 541, 743, 746
FokI GGATG 3 cut(s) 239, 279, 689
Fsp4HI GCNGC 5 cut(s) 233, 538, 541, 743, 746
FspBI CTAG 6 cut(s) 218, 716, 758, 834, 963, 987
GlaI GCGC 1 cut(s) 231
GluI GCNGC 5 cut(s) 233, 538, 541, 743, 746
GsaI CCCAGC 1 cut(s) 413
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 134, 291, 467
HapII CCGG 1 cut(s) 284
HhaI GCGC 1 cut(s) 232
Hin1II CATG 3 cut(s) 364, 885, 1084
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 9 cut(s) 8, 36, 200, 629, 646, 794, 878, 990, 1105
HpaII CCGG 1 cut(s) 284
HphI GGTGA 4 cut(s) 67, 146, 776, 865
Hpy188I TCNGA 5 cut(s) 205, 302, 628, 634, 791
Hpy188III TCNNGA 4 cut(s) 246, 350, 370, 602
Hpy99I CGWCG 2 cut(s) 247, 692
HpyCH4III ACNGT 1 cut(s) 309
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 6 cut(s) 318, 335, 553, 859, 976, 1054
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 1051
HpyF3I CTNAG 1 cut(s) 779
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 3 cut(s) 364, 885, 1084
HspAI GCGC 1 cut(s) 230
Kzo9I GATC 4 cut(s) 54, 66, 415, 712
LmnI GCTCC 3 cut(s) 183, 317, 842
Lsp1109I GCAGC 3 cut(s) 549, 552, 732
LweI GCATC 3 cut(s) 217, 708, 846
MaeI CTAG 6 cut(s) 218, 716, 758, 834, 963, 987
MaeII ACGT 1 cut(s) 24
MaeIII GTNAC 4 cut(s) 113, 170, 982, 1124
MalI GATC 4 cut(s) 56, 68, 417, 714
MboI GATC 4 cut(s) 54, 66, 415, 712
MboII GAAGA 6 cut(s) 32, 249, 382, 410, 550, 1112
MhlI GDGCHC 2 cut(s) 272, 314
MluCI AATT 7 cut(s) 122, 137, 206, 253, 386, 398, 1003
MlyI GAGTC 5 cut(s) 623, 640, 788, 872, 1099
MmeI TCCRAC 1 cut(s) 651
Mph1103I ATGCAT 1 cut(s) 1056
MseI TTAA 2 cut(s) 897, 1132
MslI CAYNNNNRTG 1 cut(s) 1085
MspA1I CMGCKG 2 cut(s) 413, 745
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 3 cut(s) 41, 285, 696
Mva1269I GAATGC 1 cut(s) 744
MvaI CCWGG 2 cut(s) 41, 696
MwoI GCNNNNNNNGC 2 cut(s) 78, 1051
NciI CCSGG 1 cut(s) 285
NdeI CATATG 1 cut(s) 1056
NdeII GATC 4 cut(s) 54, 66, 415, 712
NheI GCTAGC 1 cut(s) 833
NlaIII CATG 3 cut(s) 364, 885, 1084
NlaIV GGNNCC 4 cut(s) 179, 269, 290, 374
NmuCI GTSAC 4 cut(s) 113, 170, 982, 1124
NsiI ATGCAT 1 cut(s) 1056
NspI RCATGY 1 cut(s) 364
NspV TTCGAA 2 cut(s) 34, 86
PaqCI CACCTGC 1 cut(s) 86
PciI ACATGT 1 cut(s) 360
PctI GAATGC 1 cut(s) 744
PfeI GAWTC 4 cut(s) 8, 36, 200, 990
PflMI CCANNNNNTGG 1 cut(s) 579
PfoI TCCNGGA 1 cut(s) 39
PkrI GCNGC 5 cut(s) 234, 539, 542, 744, 747
PleI GAGTC 5 cut(s) 623, 640, 788, 872, 1099
PpsI GAGTC 5 cut(s) 623, 640, 788, 872, 1099
PscI ACATGT 1 cut(s) 360
Psp6I CCWGG 2 cut(s) 39, 694
PspFI CCCAGC 1 cut(s) 409
PspGI CCWGG 2 cut(s) 39, 694
PspN4I GGNNCC 4 cut(s) 179, 269, 290, 374
PspPI GGNCC 4 cut(s) 133, 289, 692, 885
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 1 cut(s) 967
RsaNI GTAC 1 cut(s) 966
RseI CAYNNNNRTG 1 cut(s) 1085
SaqAI TTAA 2 cut(s) 897, 1132
SatI GCNGC 5 cut(s) 233, 538, 541, 743, 746
Sau3AI GATC 4 cut(s) 54, 66, 415, 712
Sau96I GGNCC 4 cut(s) 133, 289, 692, 885
SchI GAGTC 5 cut(s) 623, 640, 788, 872, 1099
ScrFI CCNGG 3 cut(s) 41, 285, 696
SduI GDGCHC 2 cut(s) 272, 314
SfaNI GCATC 3 cut(s) 217, 708, 846
SfuI TTCGAA 2 cut(s) 34, 86
SinI GGWCC 2 cut(s) 692, 885
SmiMI CAYNNNNRTG 1 cut(s) 1085
SpeI ACTAGT 1 cut(s) 962
Sse9I AATT 7 cut(s) 122, 137, 206, 253, 386, 398, 1003
SsiI CCGC 5 cut(s) 5, 70, 192, 233, 743
SspMI CTAG 6 cut(s) 218, 716, 758, 834, 963, 987
StyD4I CCNGG 3 cut(s) 39, 283, 694
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 309
TaiI ACGT 1 cut(s) 27
TaqI TCGA 5 cut(s) 34, 86, 603, 609, 615
TasI AATT 7 cut(s) 122, 137, 206, 253, 386, 398, 1003
TatI WGTACW 1 cut(s) 965
TauI GCSGC 2 cut(s) 235, 745
TfiI GAWTC 4 cut(s) 8, 36, 200, 990
Tru1I TTAA 2 cut(s) 897, 1132
Tru9I TTAA 2 cut(s) 897, 1132
TscAI CASTG 2 cut(s) 475, 511
TseFI GTSAC 4 cut(s) 113, 170, 982, 1124
TseI GCWGC 3 cut(s) 537, 540, 745
Tsp45I GTSAC 4 cut(s) 113, 170, 982, 1124
TspDTI ATGAA 3 cut(s) 91, 656, 1113
TspGWI ACGGA 4 cut(s) 158, 705, 983, 1049
TspRI CASTG 2 cut(s) 475, 511
Van91I CCANNNNNTGG 1 cut(s) 579
VpaK11BI GGWCC 2 cut(s) 692, 885
XapI RAATTY 3 cut(s) 122, 206, 1003
XceI RCATGY 1 cut(s) 364
XspI CTAG 6 cut(s) 218, 716, 758, 834, 963, 987
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.