MD02G1216600.v1.1

Cotton fibre expressed protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009037
Physical Location & Seq
Reverse (-)
24764882 .. 24767014
2133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1216600.v1.1.491

Sequence Viewer

Length: 2133 bp
ATGGCGGACTCCATCCCCTACCGAAAACCCCACTTCCCAGTCTCCGATCCGCGCCCAGAACCTCACCGAAACCCGATTCGACACGGTAAGTCGTACACCATGCACTTTCTCCTTAAAGCTCTTACCCTTGCCGTTTTCATCGTCCTTCTTCCACTTTTTCCCTCACAAGCTCCCGAATTCATCAACCACACGATCCTCACCAAGTTCTGGGAGCTCATTCACGTTATCGTCGTCGGCATTGCCGTGTCCTACGGCTTGTTCAGCAGAAGAAATAGCGCTGAAATGGGGATTGAAAATAGCTCAAATGTAGGTAGTTCTGACTCTTACGTGCCTAGATTTTTTCCCGTTTCGTCGAGTTTTGAGGGCGAGTGTGAACAGGCATTTGGGTATGGTGAGAAAAGAGAGGGTCAGAGTTGGAATTCTGGGTATTTTGTGGGTAACCCTGTGGCAGCTGTGTCTGAATCTTGTCATGAAAGCAATGTTTTTGATGCCCAATGCAAACCCAGTTTGGGGATTTGTGAAAGTGGGGGTGAAAATTCATGTGGGTATGGAGAGAAAAATGTTACCCAAGCTTGGAACTCTCGGTATTTTCAGGGTGAATCAATGGTGTTTGTTGCTGAACCAAATTATGGTCTTGATGAATGGGGAAAACCCAGATCAATTGCTGATAATCAGCCGTTGGGATTGCCCGTTCGGAGTTTGAAATCGAGGGTAAAAAGCCATGATAGTTCTGAGTTTGTTGCTAGGGGTGAGTTTAGTTTAGGTTCTAAGGGTTCTTTTAATAGCTCTGATGGGGTTAGGAATGGGGATATGGGTCCTCTAAATTTGGAAGATAAGTTAAATGAAGCCGCTGCGTCACCTTCTCCGGTTAATTGGCGTTCGGGTTCTGGAAAGATGGAGAGGGGGAAAAGAGTAGGTGGTAATGCTCGTCCATCGCATTTGAGGCCGCTCTCCGTCGATGAAACTCAATTTGAATCAATGAAAACTCCGTCTTTCCAGTCCGCATTGTCCTTTACTTCTGAGTCTTCTTCTCAAACTAGTTCTCTGTCTTCTTCCCCAAAAGAAGACTCTTCAGCGCGCTCCATGTCTTCGGAGGTGCTTAACTCAAAGTCGGAGAAGGTAAAGAAAAGAAAGAGTTCCCATTCTCAAGGGTCTTCTTCTCCTTCAGGATTTGCATCATCACCACCAAAACCAATGAATGAAAAAGCTTCATTGAGTGCGTTGCATTCGCGGGGCTATAGCATTGGTTCTTTCCATGAGGAGGACTTGAGGAGAAGCTCAGAAAATTACTTGAAGGATTTGAGTGGGAGCAGAAGTGGAAGTGGAAGCGGAAGGGGAAGCAGAAGGGGAAGCGGAAGCGGAAGCGGAAGCGGAAGTGAGGAGGAGCAATTGAAAAGCAAAGAATTGGGGCATGGATTTTTGGGATTGAATACGAAACCTGCAAGCCTCAGTAAATCCTCAGAAAATTACTTGAAGGATTTGAGTGGAAGCAGAAGCGGAAGCAGAAGTGGAAGCGGAAGAGGAAGCAGAAGCGGAAGTGAGGAGGAGCAATTGAAAAGCAAAGAATTGGGGCAAGGTTTTTTGGGATTGAATACTAAACCTGCAAGCCTCACCAAATCCTCATCAAGGGGAAAATCGGTTAGAACTATCAGAGGTAGTAGACTTACCACTCCTGAAAAGGTTGAGAAGATGCGAGACATTGGTGATTTTATACCTATGAGAAAAGAAACAATGCAAAACGGAGGAACCAACGTGAAAGATCTCGGCACTGCAACTACTAAGCTCGATTTTGGCAATCCCTTGCAGCCAAAGCCAGAAATTCCAAAACATGGGAAGAAGCAGATGCGAGAATTTCGTGGCAATGCGGCTGCAGAGTCTGAAGAAGAAGAAGACTTGGAAAGTGAGGCGGAAAACTTTCAAGTAAGCTCGGATGATGAAGGCGAAGATGACGCTCTTGCTGCTGCTGCTGCGGCTACATGTAGTAACTCTGTGGATGTTGGAGCAGCAGACTCAGAGGTTGATAAGAAGGCGGGCGAGTTCATAGCGAAATTTAGAGAGCAGATTAGGCTTCAAAAAGTGGCATCGATGGACCGATCAAGAGCGCAACACATCTCTGCTAATTCGTTTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF761

Protein Analysis

711

Amino Acids

76.76

Weight (kDa)

8.47

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF761 PF05553 671 - 699 4.9e-10 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1447, 1609
AccB7I CCANNNNNTGG 3 cut(s) 207, 629, 1829
AccBSI CCGCTC 1 cut(s) 949
AccI GTMKAC 1 cut(s) 1660
AccII CGCG 3 cut(s) 52, 1078, 1231
AclWI GGATC 2 cut(s) 41, 187
AcsI RAATTY 7 cut(s) 176, 418, 535, 823, 1818, 1850, 2048
AcuI CTGAAG 3 cut(s) 1056, 1149, 1899
AfaI GTAC 1 cut(s) 95
AfeI AGCGCT 1 cut(s) 277
AfiI CCNNNNNNNGG 8 cut(s) 207, 509, 510, 573, 629, 1261, 1626, 1829
AflIII ACRYGT 1 cut(s) 1976
AhlI ACTAGT 1 cut(s) 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 366, 398
AloI GAACNNNNNNTCC 2 cut(s) 675, 707
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 2 cut(s) 46, 1689
AlwI GGATC 2 cut(s) 41, 187
AlwNI CAGNNNCTG 1 cut(s) 1877
Aor51HI AGCGCT 1 cut(s) 277
AoxI GGCC 1 cut(s) 944
ApeKI GCWGC 9 cut(s) 449, 851, 1804, 1868, 1958, 1961, 1964, 1967, 2003
ApoI RAATTY 7 cut(s) 176, 418, 535, 823, 1818, 1850, 2048
Asp700I GAANNNNTTC 2 cut(s) 1135, 1914
AspLEI GCGC 5 cut(s) 54, 278, 1078, 1080, 2104
AspS9I GGNCC 2 cut(s) 815, 2089
AvaII GGWCC 2 cut(s) 815, 2089
BanII GRGCYC 1 cut(s) 216
BbsI GAAGAC 6 cut(s) 1017, 1041, 1071, 1080, 1146, 1896
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 9 cut(s) 461, 838, 1816, 1855, 1945, 1948, 1951, 1954, 2015
BccI CCATC 5 cut(s) 20, 785, 889, 940, 2080
BceAI ACGGC 4 cut(s) 116, 227, 268, 661
BcoDI GTCTC 2 cut(s) 46, 1689
BcuI ACTAGT 1 cut(s) 1037
BfaI CTAG 3 cut(s) 333, 744, 1038
BfmI CTRYAG 2 cut(s) 1237, 1869
BfoI RGCGCY 1 cut(s) 279
BfuAI ACCTGC 2 cut(s) 1447, 1609
BglII AGATCT 1 cut(s) 1759
Bme18I GGWCC 2 cut(s) 815, 2089
BmgT120I GGNCC 2 cut(s) 815, 2089
BmiI GGNNCC 2 cut(s) 816, 1747
BmrI ACTGGG 2 cut(s) 32, 498
BmsI GCATC 5 cut(s) 478, 1184, 1680, 1833, 2090
BmuI ACTGGG 2 cut(s) 32, 498
BpiI GAAGAC 6 cut(s) 1017, 1041, 1071, 1080, 1146, 1896
BpuEI CTTGAG 2 cut(s) 1131, 1288
Bsa29I ATCGAT 1 cut(s) 2084
BsaAI YACGTR 1 cut(s) 328
BsaBI GATNNNNATC 1 cut(s) 1174
BsaWI WCCGGW 1 cut(s) 865
BsaXI ACNNNNNCTCC 2 cut(s) 1300, 1330
Bsc4I CCNNNNNNNGG 8 cut(s) 207, 509, 510, 573, 629, 1261, 1626, 1829
Bse1I ACTGG 3 cut(s) 38, 504, 997
Bse3DI GCAATG 3 cut(s) 237, 484, 1867
Bse8I GATNNNNATC 1 cut(s) 1174
BseCI ATCGAT 1 cut(s) 2084
BseGI GGATG 3 cut(s) 12, 1936, 1999
BseJI GATNNNNATC 1 cut(s) 1174
BseLI CCNNNNNNNGG 8 cut(s) 207, 509, 510, 573, 629, 1261, 1626, 1829
BseMI GCAATG 3 cut(s) 237, 484, 1867
BseMII CTCAG 6 cut(s) 723, 1011, 1293, 1462, 1473, 2025
BseNI ACTGG 3 cut(s) 38, 504, 997
BsePI GCGCGC 1 cut(s) 1076
BseRI GAGGAG 6 cut(s) 1274, 1285, 1394, 1397, 1556, 1559
BseXI GCAGC 9 cut(s) 461, 838, 1816, 1855, 1945, 1948, 1951, 1954, 2015
Bsh1236I CGCG 3 cut(s) 52, 1078, 1231
BshFI GGCC 1 cut(s) 946
BshVI ATCGAT 1 cut(s) 2084
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 1 cut(s) 866
BslI CCNNNNNNNGG 8 cut(s) 207, 509, 510, 573, 629, 1261, 1626, 1829
BsmAI GTCTC 2 cut(s) 46, 1689
BsmI GAATGC 1 cut(s) 1225
BsnI GGCC 1 cut(s) 946
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 5 cut(s) 46, 192, 656, 1759, 2093
BspANI GGCC 1 cut(s) 946
BspCNI CTCAG 6 cut(s) 724, 1012, 1292, 1461, 1472, 2024
BspDI ATCGAT 1 cut(s) 2084
BspFNI CGCG 3 cut(s) 52, 1078, 1231
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 816, 1747
BspMAI CTGCAG 1 cut(s) 1873
BspMI ACCTGC 2 cut(s) 1447, 1609
BspPI GGATC 2 cut(s) 41, 187
BsrBI CCGCTC 1 cut(s) 949
BsrDI GCAATG 3 cut(s) 237, 484, 1867
BsrI ACTGG 3 cut(s) 38, 504, 997
BssHII GCGCGC 1 cut(s) 1076
BssMI GATC 5 cut(s) 46, 192, 656, 1759, 2093
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 2 cut(s) 1075, 1513
BstBAI YACGTR 1 cut(s) 328
BstC8I GCNNGC 4 cut(s) 1078, 1444, 1606, 2032
BstDEI CTNAG 8 cut(s) 732, 768, 1020, 1279, 1448, 1459, 1779, 2011
BstEII GGTNACC 1 cut(s) 437
BstENI CCTNNNNNAGG 1 cut(s) 1624
BstF5I GGATG 3 cut(s) 12, 1936, 1999
BstFNI CGCG 3 cut(s) 52, 1078, 1231
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 5 cut(s) 54, 278, 1078, 1080, 2104
BstKTI GATC 5 cut(s) 49, 195, 659, 1762, 2096
BstMAI GTCTC 2 cut(s) 46, 1689
BstMBI GATC 5 cut(s) 46, 192, 656, 1759, 2093
BstMWI GCNNNNNNNGC 8 cut(s) 261, 943, 1810, 1958, 1964, 1967, 1970, 2065
BstNSI RCATGY 1 cut(s) 1980
BstPI GGTNACC 1 cut(s) 437
BstSFI CTRYAG 2 cut(s) 1237, 1869
BstUI CGCG 3 cut(s) 52, 1078, 1231
BstV1I GCAGC 9 cut(s) 461, 838, 1816, 1855, 1945, 1948, 1951, 1954, 2015
BstV2I GAAGAC 6 cut(s) 1017, 1041, 1071, 1080, 1146, 1896
BstX2I RGATCY 1 cut(s) 1759
BstYI RGATCY 1 cut(s) 1759
Bsu15I ATCGAT 1 cut(s) 2084
BsuRI GGCC 1 cut(s) 946
BsuTUI ATCGAT 1 cut(s) 2084
BtgZI GCGATG 1 cut(s) 918
BtsCI GGATG 3 cut(s) 12, 1936, 1999
BtsI GCAGTG 1 cut(s) 1767
BtsIMutI CAGTG 1 cut(s) 1767
BveI ACCTGC 2 cut(s) 1447, 1609
Cac8I GCNNGC 4 cut(s) 1078, 1444, 1606, 2032
CaiI CAGNNNCTG 1 cut(s) 1877
CciI TCATGA 1 cut(s) 469
CfoI GCGC 5 cut(s) 54, 278, 1078, 1080, 2104
Cfr13I GGNCC 2 cut(s) 815, 2089
ClaI ATCGAT 1 cut(s) 2084
CseI GACGC 2 cut(s) 843, 1958
Csp6I GTAC 1 cut(s) 94
CviAII CATG 9 cut(s) 100, 470, 540, 722, 1084, 1256, 1412, 1829, 1977
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 8 cut(s) 732, 768, 1020, 1279, 1448, 1459, 1779, 2011
DpnI GATC 5 cut(s) 48, 194, 658, 1761, 2095
DpnII GATC 5 cut(s) 46, 192, 656, 1759, 2093
Eam1104I CTCTTC 2 cut(s) 1075, 1513
EarI CTCTTC 2 cut(s) 1075, 1513
EciI GGCGGA 2 cut(s) 20, 1922
Ecl136II GAGCTC 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 216
Eco47I GGWCC 2 cut(s) 815, 2089
Eco47III AGCGCT 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 3 cut(s) 1056, 1149, 1899
Eco91I GGTNACC 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 214
EcoNI CCTNNNNNAGG 1 cut(s) 1624
EcoO109I RGGNCCY 1 cut(s) 815
EcoO65I GGTNACC 1 cut(s) 437
EcoRI GAATTC 2 cut(s) 176, 418
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 9 cut(s) 103, 473, 543, 725, 1087, 1259, 1415, 1832, 1980
FatI CATG 9 cut(s) 99, 469, 539, 721, 1083, 1255, 1411, 1828, 1976
FauI CCCGC 2 cut(s) 1224, 2023
FblI GTMKAC 1 cut(s) 1660
FokI GGATG 2 cut(s) 1943, 2006
FriOI GRGCYC 1 cut(s) 216
FspBI CTAG 3 cut(s) 333, 744, 1038
GlaI GCGC 5 cut(s) 53, 277, 1077, 1079, 2103
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 1 cut(s) 946
HapII CCGG 1 cut(s) 866
HgaI GACGC 2 cut(s) 843, 1958
HhaI GCGC 5 cut(s) 54, 278, 1078, 1080, 2104
Hin1II CATG 9 cut(s) 103, 473, 543, 725, 1087, 1259, 1415, 1832, 1980
Hin6I GCGC 5 cut(s) 52, 276, 1076, 1078, 2102
HinP1I GCGC 5 cut(s) 52, 276, 1076, 1078, 2102
HindIII AAGCTT 2 cut(s) 570, 1206
HpaII CCGG 1 cut(s) 866
Hpy166II GTNNAC 3 cut(s) 96, 374, 1661
Hpy188III TCNNGA 7 cut(s) 173, 470, 635, 888, 1167, 1673, 2097
Hpy8I GTNNAC 3 cut(s) 96, 374, 1661
Hpy99I CGWCG 4 cut(s) 233, 236, 355, 959
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 3 cut(s) 222, 327, 1752
HpyF10VI GCNNNNNNNGC 8 cut(s) 261, 943, 1810, 1958, 1964, 1967, 1970, 2065
HpyF3I CTNAG 8 cut(s) 732, 768, 1020, 1279, 1448, 1459, 1779, 2011
HpySE526I ACGT 3 cut(s) 222, 327, 1752
Hsp92II CATG 9 cut(s) 103, 473, 543, 725, 1087, 1259, 1415, 1832, 1980
HspAI GCGC 5 cut(s) 52, 276, 1076, 1078, 2102
Kzo9I GATC 5 cut(s) 46, 192, 656, 1759, 2093
LmnI GCTCC 7 cut(s) 175, 211, 1085, 1308, 1384, 1546, 2000
Lsp1109I GCAGC 9 cut(s) 461, 838, 1816, 1855, 1945, 1948, 1951, 1954, 2015
LweI GCATC 5 cut(s) 478, 1184, 1680, 1833, 2090
MaeI CTAG 3 cut(s) 333, 744, 1038
MaeII ACGT 3 cut(s) 222, 327, 1752
MaeIII GTNAC 4 cut(s) 437, 562, 855, 1982
MalI GATC 5 cut(s) 48, 194, 658, 1761, 2095
MbiI CCGCTC 1 cut(s) 949
MboI GATC 5 cut(s) 46, 192, 656, 1759, 2093
MfeI CAATTG 3 cut(s) 660, 1388, 1550
MflI RGATCY 1 cut(s) 1759
MhlI GDGCHC 1 cut(s) 216
MlyI GAGTC 6 cut(s) 2, 314, 1031, 1061, 1883, 2003
MmeI TCCRAC 3 cut(s) 395, 1092, 1978
MroXI GAANNNNTTC 2 cut(s) 1135, 1914
MseI TTAA 5 cut(s) 114, 780, 839, 870, 1101
MslI CAYNNNNRTG 1 cut(s) 242
MspA1I CMGCKG 2 cut(s) 452, 851
MspI CCGG 1 cut(s) 866
MunI CAATTG 3 cut(s) 660, 1388, 1550
Mva1269I GAATGC 1 cut(s) 1225
MvnI CGCG 3 cut(s) 52, 1078, 1231
MwoI GCNNNNNNNGC 8 cut(s) 261, 943, 1810, 1958, 1964, 1967, 1970, 2065
NdeII GATC 5 cut(s) 46, 192, 656, 1759, 2093
NlaIII CATG 9 cut(s) 103, 473, 543, 725, 1087, 1259, 1415, 1832, 1980
NlaIV GGNNCC 2 cut(s) 816, 1747
NmeAIII GCCGAG 1 cut(s) 1743
NmuCI GTSAC 1 cut(s) 855
NspI RCATGY 1 cut(s) 1980
PagI TCATGA 1 cut(s) 469
PauI GCGCGC 1 cut(s) 1076
PciI ACATGT 1 cut(s) 1976
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 240
PctI GAATGC 1 cut(s) 1225
PdmI GAANNNNTTC 2 cut(s) 1135, 1914
PfeI GAWTC 4 cut(s) 76, 461, 599, 974
PflMI CCANNNNNTGG 3 cut(s) 207, 629, 1829
PleI GAGTC 6 cut(s) 2, 314, 1030, 1061, 1882, 2003
PpsI GAGTC 6 cut(s) 2, 314, 1030, 1061, 1882, 2003
Ppu21I YACGTR 1 cut(s) 328
PpuMI RGGWCCY 1 cut(s) 815
PscI ACATGT 1 cut(s) 1976
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 815
PspEI GGTNACC 1 cut(s) 437
PspN4I GGNNCC 2 cut(s) 816, 1747
PspPI GGNCC 2 cut(s) 815, 2089
PspPPI RGGWCCY 1 cut(s) 815
PstI CTGCAG 1 cut(s) 1873
PstNI CAGNNNCTG 1 cut(s) 1877
PsuI RGATCY 1 cut(s) 1759
PteI GCGCGC 1 cut(s) 1076
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 242
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 5 cut(s) 114, 780, 839, 870, 1101
Sau3AI GATC 5 cut(s) 46, 192, 656, 1759, 2093
Sau96I GGNCC 2 cut(s) 815, 2089
SchI GAGTC 6 cut(s) 2, 314, 1031, 1061, 1883, 2003
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 5 cut(s) 478, 1184, 1680, 1833, 2090
SfcI CTRYAG 2 cut(s) 1237, 1869
SinI GGWCC 2 cut(s) 815, 2089
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 2 cut(s) 1146, 1267
SmoI CTYRAG 2 cut(s) 1146, 1267
SpeI ACTAGT 1 cut(s) 1037
SspMI CTAG 3 cut(s) 333, 744, 1038
SstI GAGCTC 1 cut(s) 216
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 3 cut(s) 225, 330, 1755
TaqI TCGA 6 cut(s) 79, 353, 707, 957, 1785, 2084
TaqII GACCGA 1 cut(s) 2106
TauI GCSGC 4 cut(s) 851, 949, 1868, 1973
TfiI GAWTC 4 cut(s) 76, 461, 599, 974
Tru1I TTAA 5 cut(s) 114, 780, 839, 870, 1101
Tru9I TTAA 5 cut(s) 114, 780, 839, 870, 1101
TscAI CASTG 1 cut(s) 1774
TseFI GTSAC 1 cut(s) 855
TseI GCWGC 9 cut(s) 449, 851, 1804, 1868, 1958, 1961, 1964, 1967, 2003
Tsp45I GTSAC 1 cut(s) 855
TspGWI ACGGA 3 cut(s) 943, 978, 1755
TspRI CASTG 1 cut(s) 1774
Van91I CCANNNNNTGG 3 cut(s) 207, 629, 1829
VpaK11BI GGWCC 2 cut(s) 815, 2089
XagI CCTNNNNNAGG 1 cut(s) 1624
XapI RAATTY 7 cut(s) 176, 418, 535, 823, 1818, 1850, 2048
XceI RCATGY 1 cut(s) 1980
XmiI GTMKAC 1 cut(s) 1660
XmnI GAANNNNTTC 2 cut(s) 1135, 1914
XspI CTAG 3 cut(s) 333, 744, 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.