MD02G1239800.v1.1

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009358
Physical Location & Seq
Reverse (-)
28813696 .. 28814166
471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1239800.v1.1.491

Sequence Viewer

Length: 444 bp
ATGGGATTGATTAGAATCGGAAAACAACTGGAACGGGCAACAGTCAAAGACCTCTTGATACCATCTAAAGGCTATTCCAAGGAAATGCAATACGAGATTGGGTGTTTGAGGCGGATACTGAAAGATTTCTATGGAAGTTTTACTTCTTCAGACGTATCCGGGCTAGTTGCAGTAGCTGAACTCATTGAAGAATTCTTGGCTGAAATTGCCAGTGACATAGATCTGAAGATAGGTACATTTGTTGAATTAGCAGAGATGTCGATTGCAGCATCGTTGGGTATACAAAGAACCTCAGATGGAATATACAGGGCGGTTCATATCTACTTGGATAAGCACAGGCACTTGACGGAGATGGAACGACAGGAAGTGTGTCAGTTTTGGATTTCAAAAAGATGTCGCCCGAAGCATGTGAACAAGCTACCAAAAATGAAATGTTGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

148

Amino Acids

16.82

Weight (kDa)

8.59

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 5 - 126 1.7e-32 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 280
AciI CCGC 2 cut(s) 112, 311
AcsI RAATTY 1 cut(s) 191
AcuI CTGAAG 2 cut(s) 132, 245
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 68
AgsI TTSAA 3 cut(s) 188, 245, 387
AluBI AGCT 2 cut(s) 176, 418
AluI AGCT 2 cut(s) 176, 418
AlwNI CAGNNNCTG 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 191
Asp700I GAANNNNTTC 1 cut(s) 125
AsuC2I CCSGG 1 cut(s) 160
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BbvI GCAGC 1 cut(s) 278
BccI CCATC 3 cut(s) 70, 290, 346
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BciVI GTATCC 2 cut(s) 108, 166
BcnI CCSGG 1 cut(s) 160
BfaI CTAG 1 cut(s) 164
BfuI GTATCC 2 cut(s) 108, 166
BglII AGATCT 1 cut(s) 220
BisI GCNGC 1 cut(s) 267
BlsI GCNGC 1 cut(s) 268
Bme1390I CCNGG 1 cut(s) 160
BmrFI CCNGG 1 cut(s) 160
BmsI GCATC 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 14
BsaJI CCNNGG 1 cut(s) 78
Bsc4I CCNNNNNNNGG 1 cut(s) 68
Bse1I ACTGG 2 cut(s) 33, 210
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 306
BseNI ACTGG 2 cut(s) 33, 210
BseXI GCAGC 1 cut(s) 278
BsiSI CCGG 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 68
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 2 cut(s) 112, 311
BspCNI CTCAG 1 cut(s) 305
BsrI ACTGG 2 cut(s) 33, 210
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 1 cut(s) 220
BssNAI GTATAC 1 cut(s) 281
BssT1I CCWWGG 1 cut(s) 78
Bst1107I GTATAC 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 43
BstDEI CTNAG 1 cut(s) 292
BstKTI GATC 1 cut(s) 223
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BstZ17I GTATAC 1 cut(s) 281
BsuI GTATCC 2 cut(s) 108, 166
BtsIMutI CAGTG 1 cut(s) 217
CaiI CAGNNNCTG 1 cut(s) 176
Csp6I GTAC 1 cut(s) 234
CviAII CATG 1 cut(s) 407
CviJI RGCY 5 cut(s) 72, 163, 176, 200, 418
CviKI_1 RGCY 5 cut(s) 72, 163, 176, 200, 418
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 1 cut(s) 292
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 78
Eco57I CTGAAG 2 cut(s) 132, 245
EcoRI GAATTC 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 1 cut(s) 410
FaiI YATR 6 cut(s) 132, 218, 281, 304, 318, 408
FalI AAGNNNNNCTT 2 cut(s) 127, 159
FatI CATG 1 cut(s) 406
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 267
FspBI CTAG 1 cut(s) 164
GluI GCNGC 1 cut(s) 267
HapII CCGG 1 cut(s) 159
Hin1II CATG 1 cut(s) 410
HinfI GANTC 1 cut(s) 15
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 2 cut(s) 281, 412
Hpy188I TCNGA 5 cut(s) 20, 151, 225, 295, 443
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 2 cut(s) 281, 412
HpyCH4III ACNGT 1 cut(s) 43
HpyCH4IV ACGT 1 cut(s) 153
HpyCH4V TGCA 3 cut(s) 88, 170, 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 1 cut(s) 292
HpySE526I ACGT 1 cut(s) 153
Hsp92II CATG 1 cut(s) 410
Kzo9I GATC 1 cut(s) 220
LpnPI CCDG 6 cut(s) 14, 172, 223, 292, 322, 347
Lsp1109I GCAGC 1 cut(s) 278
LweI GCATC 1 cut(s) 278
MaeI CTAG 1 cut(s) 164
MaeII ACGT 1 cut(s) 153
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 3 cut(s) 138, 200, 238
MflI RGATCY 1 cut(s) 220
MluCI AATT 3 cut(s) 191, 204, 245
MnlI CCTC 3 cut(s) 62, 102, 301
MroXI GAANNNNTTC 1 cut(s) 125
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 160
MwoI GCNNNNNNNGC 1 cut(s) 206
NciI CCSGG 1 cut(s) 160
NdeII GATC 1 cut(s) 220
NlaIII CATG 1 cut(s) 410
NmuCI GTSAC 1 cut(s) 212
NspI RCATGY 1 cut(s) 410
PdmI GAANNNNTTC 1 cut(s) 125
PfeI GAWTC 1 cut(s) 15
PkrI GCNGC 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 176
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SatI GCNGC 1 cut(s) 267
Sau3AI GATC 1 cut(s) 220
ScrFI CCNGG 1 cut(s) 160
SetI ASST 6 cut(s) 54, 156, 178, 235, 293, 420
SfaNI GCATC 1 cut(s) 278
Sse9I AATT 3 cut(s) 191, 204, 245
SsiI CCGC 2 cut(s) 112, 311
SspMI CTAG 1 cut(s) 164
StyD4I CCNGG 1 cut(s) 158
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 43
TaiI ACGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 260
TasI AATT 3 cut(s) 191, 204, 245
TfiI GAWTC 1 cut(s) 15
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 2 cut(s) 305, 443
TspGWI ACGGA 1 cut(s) 362
TspRI CASTG 1 cut(s) 217
XapI RAATTY 1 cut(s) 191
XceI RCATGY 1 cut(s) 410
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.