Rroxscaffold_4G00315000

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0009358
Physical Location & Seq
Reverse (-)
39210809 .. 39214453
3645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00315000.1

Sequence Viewer

Length: 1731 bp
ATGATTGATCCATTAGCACCGCGCTGCCAGCAGCCTGGACCGCTGCCGTTGCCCAAGTCTACATCGGATATGCAACTAAATGTACTTGGTGGTCAGCCATTCACTATGGACAAAGAGCTTCTGGCTGCAAAATCAGCCAAATTTGCTGCTACACTCAAAGAGAATCCGCAGGAAGATCTTGCTAACTTCCTCCAAAGTGTCCCTGCTACTGCAGACACTCTTGAGCTTGTTGCAAGATTCTATCATGGCTTTGAAATTCAAATTTGCAGTCAGAATGTTGTACAGCTCATTTGCGTTGCTGACTACTTGGACATGACTGAAAACCACAGCAAAGATAATTTATTACACAAAGCTATTTCATATTTTCAACAAAGAATCCTCCCTAGCTGGTCTGAAACTATTAAGGCTTTCCGATCCACAGAAAAGGTATTTCAACAATCCTGGAAGCTTGGATTAGTACACGAATGTTTGGATTCTATCATTAGAAAGGCAAAAGCTAGTCCCCAACTTCTGGGCGAACCAATCAAGAATTCTACTGCAAATGCTGATGATAGTGACGAGGATTATGAAGGTTATAAGCCGAATGCAAGGAGAAGGCTCTTCGATCTCAATTGGAAACCAGAGGATCTAACGACATTGTCCCTGCAGCTTTATGAGCTCCTCATGCATGCAATGAATAAGCATGGAGTTCCATCAGAATATGTGGCTGCATCTCTTTGTAACTACACTAAGAAATGGGTATTCTCTAGCAGTGGAGAGGAAGCACTCAAGAAAAAGTACCAAAAGGACATCCTTGAAGCAGTTGAGAGGCTTTTGCCTGATGAAATAGGACTCCTTCCATGCACATTGTTGTTTCAAATGCTTCGAGTTGCTATTTATTTGGAAGTTACCTCTGACTGCAGAAATGGGATTGAGATCAGAATTGGAAAACAACTTGACCAGGCAACAGTCAAAGACCTCTTAATACCATCTCACGGCTATTCTAAAGTAGTGCAATATGATATTCAATGTGTGAGAAGGATTGTGAAGGTTTTCTATGCTAATTACTCTTCTTCAAATGTAACTGACATAACTGGTCTAATAGCAGTGGCAGATCTCATTGAAGAATTCTTAGCTGAAGTTGCTAGTGACATAGACTTGAAGATATCTACATTTGTTGGATTAGCAGAGATGTTGGTTTCAGCATCCATGGGAGTAAAAACAAACTCGGATGGAATATACAGGGCTGTTGCTATATACTTAGATAAGCACAGGCACTTGACAGAATCCGAGCGAGAAGAAGTGTGTCAGGTATTGGATTTCCAAAAGATGTCCCCCCAAGCTTGTGATCATGCAGCCAAAAACGAATGCATGCCATTAAGGATGGTGGTACAGGTGTTGTTTGCGGGGCAATTGCAGCTTAGAGAAACGATCACGAAGGAGGTGCAGAGTTCGGATGAGAAATTAATAAAAGAAGAAGCAGACGAGGTGAATGCACCAAGGGTGGATTTGTGTGAGGGGAAAATGAGATCTGAGGTGGAGAAAATGAGCAAAAAGGTGATGCAGCTTGAGAGAGAGTGCAGTATGATGAGAAAAGAGATTGAGAATAGCTCTAGCCTTGTGGTGAGGAAGGAAAAACCTAGCTTGTGGAGAGAAATGAAGAGAAAGTTTGGTTGCATCAGCAGCGCTCACGATTCTAACTGCCAATTGAAGAAGAAAAAGTTGCCTCTGAAACCAAAACGTGGACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

576

Amino Acids

65.24

Weight (kDa)

6.46

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 204 - 442 1.4e-70 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 576
AccB7I CCANNNNNTGG 2 cut(s) 511, 1721
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 22
AciI CCGC 4 cut(s) 20, 41, 167, 1385
AclWI GGATC 3 cut(s) 2, 408, 633
AcsI RAATTY 5 cut(s) 140, 255, 261, 529, 1106
AcuI CTGAAG 1 cut(s) 1137
AfaI GTAC 5 cut(s) 84, 282, 459, 779, 1371
AfeI AGCGCT 1 cut(s) 1666
AfiI CCNNNNNNNGG 3 cut(s) 511, 974, 1721
AjnI CCWGG 3 cut(s) 34, 440, 939
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
Alw21I GWGCWC 1 cut(s) 660
AlwI GGATC 3 cut(s) 2, 408, 633
Aor51HI AGCGCT 1 cut(s) 1666
ApoI RAATTY 5 cut(s) 140, 255, 261, 529, 1106
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
AseI ATTAAT 1 cut(s) 1445
Asp700I GAANNNNTTC 1 cut(s) 1031
AspLEI GCGC 2 cut(s) 24, 1667
AspS9I GGNCC 1 cut(s) 38
AsuHPI GGTGA 3 cut(s) 1480, 1549, 1615
AvaII GGWCC 1 cut(s) 38
BaeI ACNNNNGTAYC 2 cut(s) 1361, 1394
BanII GRGCYC 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 660
BccI CCATC 4 cut(s) 700, 976, 1205, 1357
BceAI ACGGC 2 cut(s) 31, 991
BcgI CGANNNNNNTGC 4 cut(s) 1405, 1439, 1454, 1488
BciT130I CCWGG 3 cut(s) 36, 442, 941
BclI TGATCA 1 cut(s) 1327
BfaI CTAG 6 cut(s) 384, 498, 747, 1125, 1593, 1620
BfmI CTRYAG 3 cut(s) 210, 644, 898
BfoI RGCGCY 1 cut(s) 1668
BglII AGATCT 3 cut(s) 175, 1093, 1508
Bme1390I CCNGG 3 cut(s) 36, 442, 941
Bme18I GGWCC 1 cut(s) 38
BmgT120I GGNCC 1 cut(s) 38
BmrFI CCNGG 3 cut(s) 36, 442, 941
BmsI GCATC 4 cut(s) 719, 1193, 1530, 1665
BplI GAGNNNNNCTC 2 cut(s) 1574, 1606
BpuEI CTTGAG 3 cut(s) 242, 752, 1568
BsaBI GATNNNNATC 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 1188, 1478
Bsc4I CCNNNNNNNGG 3 cut(s) 511, 974, 1721
Bse1I ACTGG 1 cut(s) 1078
Bse3DI GCAATG 1 cut(s) 678
Bse8I GATNNNNATC 1 cut(s) 914
BseBI CCWGG 3 cut(s) 36, 442, 941
BseDI CCNNGG 2 cut(s) 1188, 1478
BseGI GGATG 5 cut(s) 789, 1184, 1216, 1368, 1441
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 3 cut(s) 511, 974, 1721
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 1 cut(s) 1503
BseNI ACTGG 1 cut(s) 1078
BseRI GAGGAG 1 cut(s) 650
BsgI GTGCAG 2 cut(s) 1445, 1579
Bsh1236I CGCG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 660
BslFI GGGAC 4 cut(s) 185, 486, 625, 1297
BslI CCNNNNNNNGG 3 cut(s) 511, 974, 1721
BsmFI GGGAC 4 cut(s) 185, 486, 625, 1297
BsmI GAATGC 3 cut(s) 589, 1352, 1477
Bsp1286I GDGCHC 1 cut(s) 660
Bsp1407I TGTACA 1 cut(s) 280
Bsp19I CCATGG 1 cut(s) 1188
BspACI CCGC 4 cut(s) 20, 41, 167, 1385
BspCNI CTCAG 1 cut(s) 1504
BspFNI CGCG 1 cut(s) 22
BspMAI CTGCAG 3 cut(s) 214, 648, 902
BspPI GGATC 3 cut(s) 2, 408, 633
BspQI GCTCTTC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 678
BsrGI TGTACA 1 cut(s) 280
BsrI ACTGG 1 cut(s) 1078
BssECI CCNNGG 2 cut(s) 1188, 1478
BssT1I CCWWGG 2 cut(s) 1188, 1478
Bst2UI CCWGG 3 cut(s) 36, 442, 941
Bst4CI ACNGT 2 cut(s) 949, 1728
Bst6I CTCTTC 3 cut(s) 605, 1054, 1634
BstAUI TGTACA 1 cut(s) 280
BstC8I GCNNGC 3 cut(s) 29, 669, 1352
BstDEI CTNAG 5 cut(s) 729, 1111, 1240, 1400, 1512
BstDSI CCRYGG 1 cut(s) 1188
BstF5I GGATG 5 cut(s) 789, 1184, 1216, 1368, 1441
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 1668
BstHHI GCGC 2 cut(s) 24, 1667
BstNI CCWGG 3 cut(s) 36, 442, 941
BstNSI RCATGY 2 cut(s) 671, 1354
BstSCI CCNGG 3 cut(s) 34, 440, 939
BstSFI CTRYAG 3 cut(s) 210, 644, 898
BstUI CGCG 1 cut(s) 22
BstX2I RGATCY 4 cut(s) 175, 625, 1093, 1508
BstXI CCANNNNNNTGG 1 cut(s) 35
BstYI RGATCY 4 cut(s) 175, 625, 1093, 1508
BtgI CCRYGG 1 cut(s) 1188
BtsCI GGATG 5 cut(s) 789, 1184, 1216, 1368, 1441
BtsI GCAGTG 2 cut(s) 757, 1092
BtsIMutI CAGTG 2 cut(s) 757, 1092
Cac8I GCNNGC 3 cut(s) 29, 669, 1352
CfoI GCGC 2 cut(s) 24, 1667
Cfr13I GGNCC 1 cut(s) 38
Csp6I GTAC 5 cut(s) 83, 281, 458, 778, 1370
CviAII CATG 9 cut(s) 245, 313, 664, 668, 683, 840, 1189, 1331, 1351
CviQI GTAC 5 cut(s) 83, 281, 458, 778, 1370
DdeI CTNAG 5 cut(s) 729, 1111, 1240, 1400, 1512
Eam1104I CTCTTC 3 cut(s) 605, 1054, 1634
EarI CTCTTC 3 cut(s) 605, 1054, 1634
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 1188, 1478
Eco24I GRGCYC 1 cut(s) 660
Eco32I GATATC 1 cut(s) 1146
Eco47I GGWCC 1 cut(s) 38
Eco47III AGCGCT 1 cut(s) 1666
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 1137
EcoICRI GAGCTC 1 cut(s) 658
EcoRI GAATTC 2 cut(s) 529, 1106
EcoRII CCWGG 3 cut(s) 34, 440, 939
EcoRV GATATC 1 cut(s) 1146
EcoT14I CCWWGG 2 cut(s) 1188, 1478
EcoT22I ATGCAT 2 cut(s) 669, 1352
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 2 cut(s) 1188, 1478
FaeI CATG 9 cut(s) 248, 316, 667, 671, 686, 843, 1192, 1334, 1354
FaqI GGGAC 4 cut(s) 185, 486, 625, 1297
FatI CATG 9 cut(s) 244, 312, 663, 667, 682, 839, 1188, 1330, 1350
FauI CCCGC 1 cut(s) 1378
FbaI TGATCA 1 cut(s) 1327
FblI GTMKAC 1 cut(s) 59
FokI GGATG 5 cut(s) 776, 1171, 1223, 1375, 1448
FriOI GRGCYC 1 cut(s) 660
FspBI CTAG 6 cut(s) 384, 498, 747, 1125, 1593, 1620
GlaI GCGC 2 cut(s) 23, 1666
HaeII RGCGCY 1 cut(s) 1668
HhaI GCGC 2 cut(s) 24, 1667
Hin1II CATG 9 cut(s) 248, 316, 667, 671, 686, 843, 1192, 1334, 1354
Hin6I GCGC 2 cut(s) 22, 1665
HinP1I GCGC 2 cut(s) 22, 1665
HindIII AAGCTT 2 cut(s) 446, 1320
HinfI GANTC 7 cut(s) 163, 237, 375, 473, 831, 1265, 1673
HphI GGTGA 3 cut(s) 1480, 1549, 1615
Hpy166II GTNNAC 3 cut(s) 60, 460, 1724
Hpy188III TCNNGA 5 cut(s) 221, 526, 769, 1414, 1670
Hpy8I GTNNAC 3 cut(s) 60, 460, 1724
HpyAV CCTTC 7 cut(s) 563, 588, 845, 1011, 1021, 1411, 1603
HpyCH4III ACNGT 2 cut(s) 949, 1728
HpyCH4IV ACGT 1 cut(s) 1720
HpyF3I CTNAG 5 cut(s) 729, 1111, 1240, 1400, 1512
HpySE526I ACGT 1 cut(s) 1720
Hsp92II CATG 9 cut(s) 248, 316, 667, 671, 686, 843, 1192, 1334, 1354
HspAI GCGC 2 cut(s) 22, 1665
Ksp22I TGATCA 1 cut(s) 1327
LguI GCTCTTC 1 cut(s) 605
LmnI GCTCC 1 cut(s) 663
LweI GCATC 4 cut(s) 719, 1193, 1530, 1665
MaeI CTAG 6 cut(s) 384, 498, 747, 1125, 1593, 1620
MaeII ACGT 1 cut(s) 1720
MaeIII GTNAC 5 cut(s) 554, 719, 886, 1060, 1127
MfeI CAATTG 3 cut(s) 610, 1391, 1685
MflI RGATCY 4 cut(s) 175, 625, 1093, 1508
MhlI GDGCHC 1 cut(s) 660
MlyI GAGTC 1 cut(s) 825
MmeI TCCRAC 1 cut(s) 1138
Mph1103I ATGCAT 2 cut(s) 669, 1352
MroXI GAANNNNTTC 1 cut(s) 1031
MseI TTAA 4 cut(s) 402, 962, 1358, 1445
MspA1I CMGCKG 1 cut(s) 43
MspR9I CCNGG 3 cut(s) 36, 442, 941
MunI CAATTG 3 cut(s) 610, 1391, 1685
Mva1269I GAATGC 3 cut(s) 589, 1352, 1477
MvaI CCWGG 3 cut(s) 36, 442, 941
MvnI CGCG 1 cut(s) 22
NcoI CCATGG 1 cut(s) 1188
NlaIII CATG 9 cut(s) 248, 316, 667, 671, 686, 843, 1192, 1334, 1354
NmuCI GTSAC 2 cut(s) 554, 1127
NsiI ATGCAT 2 cut(s) 669, 1352
NspI RCATGY 2 cut(s) 671, 1354
PaeI GCATGC 2 cut(s) 671, 1354
PciSI GCTCTTC 1 cut(s) 605
PctI GAATGC 3 cut(s) 589, 1352, 1477
PdmI GAANNNNTTC 1 cut(s) 1031
PfeI GAWTC 6 cut(s) 163, 237, 375, 473, 1265, 1673
PflFI GACNNNGTC 1 cut(s) 637
PflMI CCANNNNNTGG 2 cut(s) 511, 1721
PfoI TCCNGGA 1 cut(s) 440
PleI GAGTC 1 cut(s) 825
PpsI GAGTC 1 cut(s) 825
PshBI ATTAAT 1 cut(s) 1445
PsiI TTATAA 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 660
Psp6I CCWGG 3 cut(s) 34, 440, 939
PspGI CCWGG 3 cut(s) 34, 440, 939
PspPI GGNCC 1 cut(s) 38
PstI CTGCAG 3 cut(s) 214, 648, 902
PsuI RGATCY 4 cut(s) 175, 625, 1093, 1508
PsyI GACNNNGTC 1 cut(s) 637
RsaI GTAC 5 cut(s) 84, 282, 459, 779, 1371
RsaNI GTAC 5 cut(s) 83, 281, 458, 778, 1370
SacI GAGCTC 1 cut(s) 660
SapI GCTCTTC 1 cut(s) 605
SaqAI TTAA 4 cut(s) 402, 962, 1358, 1445
Sau96I GGNCC 1 cut(s) 38
SchI GAGTC 1 cut(s) 825
ScrFI CCNGG 3 cut(s) 36, 442, 941
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 4 cut(s) 719, 1193, 1530, 1665
SfcI CTRYAG 3 cut(s) 210, 644, 898
SinI GGWCC 1 cut(s) 38
SmlI CTYRAG 3 cut(s) 221, 767, 1547
SmoI CTYRAG 3 cut(s) 221, 767, 1547
SphI GCATGC 2 cut(s) 671, 1354
SsiI CCGC 4 cut(s) 20, 41, 167, 1385
SspMI CTAG 6 cut(s) 384, 498, 747, 1125, 1593, 1620
SstI GAGCTC 1 cut(s) 660
StyD4I CCNGG 3 cut(s) 34, 440, 939
StyI CCWWGG 2 cut(s) 1188, 1478
TaaI ACNGT 2 cut(s) 949, 1728
TaiI ACGT 1 cut(s) 1723
TaqI TCGA 2 cut(s) 603, 865
TatI WGTACW 3 cut(s) 82, 280, 457
TfiI GAWTC 6 cut(s) 163, 237, 375, 473, 1265, 1673
Tru1I TTAA 4 cut(s) 402, 962, 1358, 1445
Tru9I TTAA 4 cut(s) 402, 962, 1358, 1445
TscAI CASTG 2 cut(s) 757, 1092
TseFI GTSAC 2 cut(s) 554, 1127
Tsp45I GTSAC 2 cut(s) 554, 1127
TspDTI ATGAA 5 cut(s) 348, 582, 689, 837, 1652
TspRI CASTG 2 cut(s) 757, 1092
Tth111I GACNNNGTC 1 cut(s) 637
Van91I CCANNNNNTGG 2 cut(s) 511, 1721
VpaK11BI GGWCC 1 cut(s) 38
VspI ATTAAT 1 cut(s) 1445
XapI RAATTY 5 cut(s) 140, 255, 261, 529, 1106
XceI RCATGY 2 cut(s) 671, 1354
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 1031
XspI CTAG 6 cut(s) 384, 498, 747, 1125, 1593, 1620
Zsp2I ATGCAT 2 cut(s) 669, 1352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.