MD02G1239900.v1.1

NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009358
Physical Location & Seq
Reverse (-)
28820408 .. 28821466
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1239900.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCAAAGAGACAATGCAAAGATTTATCATCCTGGTTGTCTAAGCGTACATTGGCACCAGATTTGCAACTCAACGTACTTGGTGCGCCGTTCATGGACAAAGTTCTTCTGGCTGAAAAATCAGCCAAAGTTGCTGCCTTACTCAAACAGAAATCGCAGGATGATCTTTCTCGCTTCCTTAGAAATATCCCAGCTGACACAGACACTCTGGAGCTTGTTGCCAAATTCTACCGCGGCTTTGAAATTCAGATGTCCCCTGAGAATATTGTACCTCTCATTTGTGTAGCTCACTACTTGGAGATGACTGAAAACCACAGCAAAAATAATTTGTTAAGTAAGGCGCTTTCTTATTTTCAAGAAAGAATCCTCCCGAGCTGGAATGAAACTGTGAAGGCTTTCCGAGCCACAGAAAAGTTCCTCCAACAGTCTGTGATGCTCGGTTTAATCGATGCCTGTATAGATTCTATCATTCAAAAGGCACTAGCTAATCCCAGCCTTCTCGGAGAGCCAATCAACAATTTGATGAAGTATGATGACGATAGTGAGGATGATGACGAAGAAGGCTATAGGCCGAGCGCAAGGAGAAGGCTCTTTGTTCTTGATTGGCAATCAGAAGACTTGACAACACTGTCCCTCCCGATCTATGAGCCTGTCATCCTTGGAATGAATAAGCATGACGTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB NPH3

Protein Analysis

229

Amino Acids

25.96

Weight (kDa)

5.2

Isoelectric Point (pI)

65.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 628
AccB1I GGYRCC 1 cut(s) 55
AccII CGCG 1 cut(s) 234
AciI CCGC 2 cut(s) 232, 234
AcsI RAATTY 2 cut(s) 224, 243
AfaI GTAC 3 cut(s) 49, 78, 270
AgsI TTSAA 3 cut(s) 242, 356, 473
AjnI CCWGG 1 cut(s) 32
AluBI AGCT 5 cut(s) 194, 214, 287, 375, 485
AluI AGCT 5 cut(s) 194, 214, 287, 375, 485
Alw26I GTCTC 1 cut(s) 4
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 1 cut(s) 569
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 2 cut(s) 224, 243
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 3 cut(s) 88, 343, 578
AvaI CYCGRG 1 cut(s) 370
BanI GGYRCC 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 121
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 4
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 565
BfoI RGCGCY 1 cut(s) 344
BisI GCNGC 2 cut(s) 135, 235
BlsI GCNGC 2 cut(s) 136, 236
Bme1390I CCNGG 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 57
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 2 cut(s) 423, 439
BpiI GAAGAC 1 cut(s) 621
BpmI CTGGAG 1 cut(s) 230
Bsa29I ATCGAT 1 cut(s) 447
BsaJI CCNNGG 2 cut(s) 232, 658
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
BseBI CCWGG 1 cut(s) 34
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 232, 658
BseGI GGATG 4 cut(s) 29, 166, 553, 654
BseMII CTCAG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 121
BseYI CCCAGC 2 cut(s) 190, 491
Bsh1236I CGCG 1 cut(s) 234
BshFI GGCC 1 cut(s) 571
BshNI GGYRCC 1 cut(s) 55
BshVI ATCGAT 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 370
BslFI GGGAC 2 cut(s) 238, 616
BsmAI GTCTC 1 cut(s) 4
BsmFI GGGAC 2 cut(s) 238, 616
BsnI GGCC 1 cut(s) 571
BsoBI CYCGRG 1 cut(s) 370
Bsp143I GATC 2 cut(s) 163, 639
BspACI CCGC 2 cut(s) 232, 234
BspANI GGCC 1 cut(s) 571
BspCNI CTCAG 1 cut(s) 250
BspDI ATCGAT 1 cut(s) 447
BspFNI CGCG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 57
BspT107I GGYRCC 1 cut(s) 55
BssECI CCNNGG 2 cut(s) 232, 658
BssMI GATC 2 cut(s) 163, 639
BssT1I CCWWGG 1 cut(s) 658
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 3 cut(s) 388, 426, 630
BstDEI CTNAG 3 cut(s) 42, 179, 258
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 4 cut(s) 29, 166, 553, 654
BstFNI CGCG 1 cut(s) 234
BstH2I RGCGCY 1 cut(s) 344
BstHHI GCGC 3 cut(s) 88, 343, 578
BstKTI GATC 2 cut(s) 166, 642
BstMAI GTCTC 1 cut(s) 4
BstMBI GATC 2 cut(s) 163, 639
BstMWI GCNNNNNNNGC 2 cut(s) 131, 401
BstNI CCWGG 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 32
BstSFI CTRYAG 1 cut(s) 565
BstUI CGCG 1 cut(s) 234
BstV1I GCAGC 1 cut(s) 121
BstV2I GAAGAC 1 cut(s) 621
Bsu15I ATCGAT 1 cut(s) 447
BsuRI GGCC 1 cut(s) 571
BsuTUI ATCGAT 1 cut(s) 447
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 4 cut(s) 29, 166, 553, 654
BtsIMutI CAGTG 1 cut(s) 626
CfoI GCGC 3 cut(s) 88, 343, 578
Cfr42I CCGCGG 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 3 cut(s) 48, 77, 269
CviAII CATG 2 cut(s) 94, 674
CviQI GTAC 3 cut(s) 48, 77, 269
DdeI CTNAG 3 cut(s) 42, 179, 258
DpnI GATC 2 cut(s) 165, 641
DpnII GATC 2 cut(s) 163, 639
DrdI GACNNNNNNGTC 1 cut(s) 628
DseDI GACNNNNNNGTC 1 cut(s) 628
Eco130I CCWWGG 1 cut(s) 658
Eco88I CYCGRG 1 cut(s) 370
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 1 cut(s) 658
ErhI CCWWGG 1 cut(s) 658
FaeI CATG 2 cut(s) 97, 677
FaiI YATR 7 cut(s) 95, 458, 531, 567, 645, 675, 685
FaqI GGGAC 2 cut(s) 238, 616
FatI CATG 2 cut(s) 93, 673
Fnu4HI GCNGC 2 cut(s) 135, 235
FokI GGATG 4 cut(s) 16, 173, 560, 641
Fsp4HI GCNGC 2 cut(s) 135, 235
FspBI CTAG 1 cut(s) 482
GlaI GCGC 3 cut(s) 87, 342, 577
GluI GCNGC 2 cut(s) 135, 235
GsaI CCCAGC 2 cut(s) 194, 495
GsuI CTGGAG 1 cut(s) 230
HaeII RGCGCY 1 cut(s) 344
HaeIII GGCC 1 cut(s) 571
HhaI GCGC 3 cut(s) 88, 343, 578
Hin1II CATG 2 cut(s) 97, 677
Hin6I GCGC 3 cut(s) 86, 341, 576
HinP1I GCGC 3 cut(s) 86, 341, 576
HinfI GANTC 2 cut(s) 363, 461
Hpy188I TCNGA 4 cut(s) 249, 401, 503, 613
Hpy188III TCNNGA 5 cut(s) 209, 356, 370, 599, 637
HpyAV CCTTC 4 cut(s) 385, 506, 554, 579
HpyCH4III ACNGT 3 cut(s) 388, 426, 630
HpyCH4IV ACGT 2 cut(s) 75, 678
HpyCH4V TGCA 2 cut(s) 18, 67
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 401
HpyF3I CTNAG 3 cut(s) 42, 179, 258
HpySE526I ACGT 2 cut(s) 75, 678
Hsp92II CATG 2 cut(s) 97, 677
HspAI GCGC 3 cut(s) 86, 341, 576
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 2 cut(s) 163, 639
LmnI GCTCC 1 cut(s) 211
Lsp1109I GCAGC 1 cut(s) 121
LweI GCATC 2 cut(s) 423, 439
MaeI CTAG 1 cut(s) 482
MaeII ACGT 2 cut(s) 75, 678
MalI GATC 2 cut(s) 165, 641
MboI GATC 2 cut(s) 163, 639
MboII GAAGA 3 cut(s) 98, 569, 626
MluCI AATT 4 cut(s) 224, 243, 325, 517
MmeI TCCRAC 1 cut(s) 445
MnlI CCTC 5 cut(s) 282, 377, 428, 538, 644
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 2 cut(s) 332, 443
MspA1I CMGCKG 2 cut(s) 194, 234
MspR9I CCNGG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 234
MwoI GCNNNNNNNGC 2 cut(s) 131, 401
NdeII GATC 2 cut(s) 163, 639
NlaIII CATG 2 cut(s) 97, 677
NlaIV GGNNCC 1 cut(s) 57
NmeAIII GCCGAG 1 cut(s) 597
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 2 cut(s) 363, 461
PkrI GCNGC 2 cut(s) 136, 236
Psp6I CCWGG 1 cut(s) 32
PspFI CCCAGC 2 cut(s) 190, 491
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 1 cut(s) 57
PvuII CAGCTG 1 cut(s) 194
RsaI GTAC 3 cut(s) 49, 78, 270
RsaNI GTAC 3 cut(s) 48, 77, 269
SacII CCGCGG 1 cut(s) 235
SaqAI TTAA 2 cut(s) 332, 443
SatI GCNGC 2 cut(s) 135, 235
Sau3AI GATC 2 cut(s) 163, 639
ScrFI CCNGG 1 cut(s) 34
SetI ASST 8 cut(s) 78, 196, 216, 274, 289, 377, 487, 681
SfaNI GCATC 2 cut(s) 423, 439
SfcI CTRYAG 1 cut(s) 565
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
Sse9I AATT 4 cut(s) 224, 243, 325, 517
SsiI CCGC 2 cut(s) 232, 234
SspI AATATT 1 cut(s) 265
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 1 cut(s) 32
StyI CCWWGG 1 cut(s) 658
TaaI ACNGT 3 cut(s) 388, 426, 630
TaiI ACGT 2 cut(s) 78, 681
TaqI TCGA 1 cut(s) 447
TasI AATT 4 cut(s) 224, 243, 325, 517
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 2 cut(s) 363, 461
Tru1I TTAA 2 cut(s) 332, 443
Tru9I TTAA 2 cut(s) 332, 443
TscAI CASTG 1 cut(s) 633
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 82, 396, 539, 680
TspRI CASTG 1 cut(s) 633
XapI RAATTY 2 cut(s) 224, 243
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.