Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003765

Physical Location & Seq

Genomic Coordinates Forward (+)

29003658 .. 29009961

Gene Structure 6304 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1241200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGTCGAGCGACGGCGACGACGAGGACGAGCTCCTCCAGATGGCTCTCAAGGAGCAAGCCCAGCGCGACGTCAACTACAGGAAGCCCACCTCCAGCAACTCCCGTTCTGCCCCCGTAGCCAATTACGTCCAGCAGCCCCACCAGCAACCGCCGAGGAAACCCGCCGCTTCTCCTGCCCCCAATCACGCCGGCAAAACCAGCAACAGGCGGCGGGTGGTGGACGACGACGACGAATCTGATGTCGACATGCTCAGCATATCCAGCGGCGATGAGGACTCCACCGCCAAAGATCAGCAGCGCGCGCGGTATCGAGGCGGCAGCGCCGCTTCTGCTGCTGCTGCCGCCAGACGTGGTTCTCGGGGAGATGATGATGCCGCTTGGGACGGCGGAGAGCCCGGTATCTGGAAGCACGTCGACGAGGCCGAGCTTGCTCGTAGGGTTCGTGAAATGCGAGAGACAAGATCAGCACCGGTGGCCCAAAAGGTTGAGCGCAAAGTTTCGACAGCAGGCTTGGCACGAAAGGGGCTTAACAATTTGCAGTCTTTCCCACGGGGCATGGAATGCATTGATCCCCTAGGCTTGGGGATCATAGACAACAAAACGTTAAGATTGATTACTGAGTCGTCGGACCATTTTCCGTCCAAGGATGACAAACTGGATAACAACCTTCGTGAGAAGTTGTTGTATTTCTCTGAGAAATTTGATGCCAAGCTCTTTATATCACGGATACACCAAGATACTGGTGCAGCAGATTTGGAGGCGGGTGCTCTTGCTCTAAAAAGTGATCTTAAAGGACGGACTCAGCAGAGAAAACAGCTGGTCAAAGACAATTTTGACTGCTTTGTCTCTTGCAAAACCACAATTGATGATATTGAGTCAAAGTTAAAACGGATTGAAGAAGACCCTGAAGGTTCAGGCACTTCTCATTTGTTTAACTGTATGCAAGGAGTGAGTTCGTTGGCTAATCGTGCATTCCAGCCTCTATTTGAGAGACAGGCTCAAGCTGAGAAGATCAGGTCTGTGCAAGGAATGCTACAGAGGTTCCGAACGCTATTCAACTTGCCCAGTACAATTCGTGGTAGCATAAGCAAGGGAGAATATGACTTGGCAGTTAGGGAATACAAGAAGGCCAAATCGATAGCTCTGCCTTCTCATGTGGGAATACTGAAACGTGTACTTGAAGAGGTTGAAAAAGTGATGCATGAGTTTAAAGGGATGCTTTACAAGTCTATGGAAGATCCACAAATAGATTTAACAAATCTTGAAAACACAGTGAGGCTGTTATTGGAGCTGGAGCCTGAGTCAGATCCTGTATGGCACTATTTAAATATACAGAATAACAGAATTCGAGGTTTGCTTGAGAAGTGCACCTTAGATCATGAAGCTAGGATGGAGACTTTACATAATGAGTTACGTGAAAGGGCTCTGTCTGATGCAAGATGGAGGCAGATTCAAGAAGATTTAAATGAATCTTCGGATGTCAACTATTCTTTTGGGGACAACCACCTTCCAGTAGATTCTCTGCCAGTAGACTTGACAGGTGAAGAAGTTGATGCTCTTAGAGGGAGGTATATTCGCAGATTAACTGCTGTACTTATCCATCACATACCAGCTTTCTGGAAAGTGGCACTTTCTGTTTTCAGTGGAAAGTTCGCAAAGCTGCTTTATGATAATATGCATGGAGAACTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000145 GO:0001927 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0005938 GO:0006810 GO:0006887 GO:0006904 GO:0007154 GO:0008037 GO:0008150 GO:0009506 GO:0009856 GO:0009875 GO:0009987 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0022414 GO:0022607 GO:0030054 GO:0031982 GO:0032501 GO:0032940 GO:0032991 GO:0034622 GO:0043226 GO:0043227 GO:0043230 GO:0043933 GO:0044085 GO:0044421 GO:0044424 GO:0044444 GO:0044448 GO:0044464 GO:0044706 GO:0046903 GO:0048278 GO:0048544 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051704 GO:0055044 GO:0060321 GO:0065003 GO:0070062 GO:0071840 GO:0071944 GO:0099023 GO:0099568 GO:0140029 GO:0140056 GO:1903561

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

Sec5

Protein Analysis

564

Amino Acids

63.52

Weight (kDa)

6.08

Isoelectric Point (pI)

48.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec5	PF15469	190 - 553	7.4e-88	Exocyst complex component Sec5
Dor1	PF04124	256 - 433	1.1e-07	Dor1-like family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21170 AT1G21170 AT1G76850
fragaria_vesca	FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280
malus_domestica	MD02G1241200.v1.1 MD02G1241400.v1.1 MD07G1075500.v1.1
prunus_persica	Prupe.8G058700_v2.0.a1 Prupe.8G058700_v2.0.a1
pyrus_communis	pycom02g20640 pycom02g20660 pycom02g20670 pycom02g20680 pycom02g20750 pycom02g20760 pycom07g05950
rosa_chinensis	RchiOBHm_Chr1g0332001
rosa_laevigata	RLG00000029699
rosa_multiflora	Rmu_co8474765.1_g000001 Rmu_sc0003358.1_g000014
rosa_roxburghii	Rroxscaffold_4G00318510
rosa_rugosa	Rorug01G0097700
rosa_samantha	Rh1AG121600 Rh1BG093100 Rh1CG116400 Rh1DG127300
rosa_wichuraiana	Rw1G009920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	842
AatII	GACGTC	1 cut(s)	72
AccI	GTMKAC	3 cut(s)	243, 414, 1530
AccII	CGCG	4 cut(s)	66, 300, 302, 304
AclI	AACGTT	1 cut(s)	602
AclWI	GGATC	4 cut(s)	563, 593, 1232, 1301
AcsI	RAATTY	2 cut(s)	698, 1344
AcuI	CTGAAG	1 cut(s)	927
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	3 cut(s)	1069, 1176, 1593
AfiI	CCNNNNNNNGG	4 cut(s)	40, 204, 402, 580
AflIII	ACRYGT	1 cut(s)	1171
AgeI	ACCGGT	1 cut(s)	469
AgsI	TTSAA	6 cut(s)	896, 1057, 1181, 1190, 1265, 1454
AjiI	CACGTC	2 cut(s)	350, 412
Alw21I	GWGCWC	3 cut(s)	33, 769, 1370
Alw26I	GTCTC	4 cut(s)	449, 850, 985, 1388
Alw44I	GTGCAC	1 cut(s)	1366
AlwI	GGATC	4 cut(s)	563, 593, 1232, 1301
AlwNI	CAGNNNCTG	1 cut(s)	1310
Ama87I	CYCGRG	1 cut(s)	357
AoxI	GGCC	3 cut(s)	420, 474, 1128
ApaLI	GTGCAC	1 cut(s)	1366
ApeKI	GCWGC	8 cut(s)	133, 295, 318, 332, 335, 338, 746, 1660
ApoI	RAATTY	2 cut(s)	698, 1344
ArsI	GACNNNNNNTTYG	2 cut(s)	225, 257
AsiGI	ACCGGT	1 cut(s)	469
AspA2I	CCTAGG	1 cut(s)	574
AspLEI	GCGC	6 cut(s)	66, 300, 302, 304, 323, 492
AspS9I	GGNCC	2 cut(s)	475, 628
AsuC2I	CCSGG	1 cut(s)	396
AsuHPI	GGTGA	1 cut(s)	1553
AvaI	CYCGRG	1 cut(s)	357
AvaII	GGWCC	1 cut(s)	628
AvrII	CCTAGG	1 cut(s)	574
BaeGI	GKGCMC	1 cut(s)	1370
BanII	GRGCYC	3 cut(s)	33, 396, 1426
BbsI	GAAGAC	1 cut(s)	906
Bbv12I	GWGCWC	3 cut(s)	33, 769, 1370
BbvI	GCAGC	8 cut(s)	145, 307, 319, 322, 325, 330, 758, 1647
BccI	CCATC	4 cut(s)	34, 1384, 1434, 1608
BceAI	ACGGC	2 cut(s)	28, 400
BcgI	CGANNNNNNTGC	2 cut(s)	1126, 1160
BciVI	GTATCC	1 cut(s)	720
BcnI	CCSGG	1 cut(s)	396
BcoDI	GTCTC	4 cut(s)	449, 850, 985, 1388
BfaI	CTAG	2 cut(s)	575, 1386
BfmI	CTRYAG	2 cut(s)	76, 1034
BfoI	RGCGCY	1 cut(s)	324
BfuI	GTATCC	1 cut(s)	720
BlnI	CCTAGG	1 cut(s)	574
BlpI	GCTNAGC	1 cut(s)	251
Bme1390I	CCNGG	1 cut(s)	396
Bme18I	GGWCC	1 cut(s)	628
BmeT110I	CYCGRG	1 cut(s)	357
BmgBI	CACGTC	2 cut(s)	350, 412
BmgT120I	GGNCC	2 cut(s)	475, 628
BmiI	GGNNCC	2 cut(s)	1043, 1296
BmrFI	CCNGG	1 cut(s)	396
BmrI	ACTGGG	1 cut(s)	1059
BmsI	GCATC	6 cut(s)	361, 694, 1188, 1206, 1423, 1543
BmuI	ACTGGG	1 cut(s)	1059
BpiI	GAAGAC	1 cut(s)	906
BplI	GAGNNNNNCTC	2 cut(s)	982, 1014
BpmI	CTGGAG	3 cut(s)	20, 76, 1313
Bpu1102I	GCTNAGC	1 cut(s)	251
BpuEI	CTTGAG	3 cut(s)	32, 984, 1379
BpuMI	CCSGG	1 cut(s)	396
Bsa29I	ATCGAT	1 cut(s)	1136
BsaAI	YACGTR	1 cut(s)	1415
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	4 cut(s)	152, 548, 574, 642
BsaWI	WCCGGW	1 cut(s)	469
BsaXI	ACNNNNNCTCC	2 cut(s)	1286, 1316
Bsc4I	CCNNNNNNNGG	4 cut(s)	40, 204, 402, 580
Bse118I	RCCGGY	2 cut(s)	188, 469
Bse1I	ACTGG	5 cut(s)	660, 745, 1065, 1511, 1526
BseCI	ATCGAT	1 cut(s)	1136
BseDI	CCNNGG	4 cut(s)	152, 548, 574, 642
BseGI	GGATG	4 cut(s)	652, 1221, 1395, 1483
BseLI	CCNNNNNNNGG	4 cut(s)	40, 204, 402, 580
BseMII	CTCAG	6 cut(s)	265, 609, 684, 815, 996, 1290
BseNI	ACTGG	5 cut(s)	660, 745, 1065, 1511, 1526
BsePI	GCGCGC	2 cut(s)	298, 300
BseRI	GAGGAG	1 cut(s)	23
BseSI	GKGCMC	1 cut(s)	1370
BseXI	GCAGC	8 cut(s)	145, 307, 319, 322, 325, 330, 758, 1647
BseYI	CCCAGC	1 cut(s)	60
BsgI	GTGCAG	1 cut(s)	765
Bsh1236I	CGCG	4 cut(s)	66, 300, 302, 304
BshFI	GGCC	3 cut(s)	422, 476, 1130
BshTI	ACCGGT	1 cut(s)	469
BshVI	ATCGAT	1 cut(s)	1136
BsiHKAI	GWGCWC	3 cut(s)	33, 769, 1370
BsiHKCI	CYCGRG	1 cut(s)	357
BsiSI	CCGG	3 cut(s)	189, 396, 470
BslFI	GGGAC	2 cut(s)	395, 1511
BslI	CCNNNNNNNGG	4 cut(s)	40, 204, 402, 580
BsmAI	GTCTC	4 cut(s)	449, 850, 985, 1388
BsmFI	GGGAC	2 cut(s)	395, 1511
BsmI	GAATGC	3 cut(s)	566, 971, 1035
BsnI	GGCC	3 cut(s)	422, 476, 1130
BsoBI	CYCGRG	1 cut(s)	357
Bsp1286I	GDGCHC	5 cut(s)	33, 396, 769, 1370, 1426
Bsp143I	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
Bsp1720I	GCTNAGC	1 cut(s)	251
BspANI	GGCC	3 cut(s)	422, 476, 1130
BspCNI	CTCAG	6 cut(s)	264, 610, 685, 814, 997, 1291
BspDI	ATCGAT	1 cut(s)	1136
BspFNI	CGCG	4 cut(s)	66, 300, 302, 304
BspHI	TCATGA	1 cut(s)	1378
BspLI	GGNNCC	2 cut(s)	1043, 1296
BspPI	GGATC	4 cut(s)	563, 593, 1232, 1301
BsrFI	RCCGGY	2 cut(s)	188, 469
BsrI	ACTGG	5 cut(s)	660, 745, 1065, 1511, 1526
BssAI	RCCGGY	2 cut(s)	188, 469
BssECI	CCNNGG	4 cut(s)	152, 548, 574, 642
BssHII	GCGCGC	2 cut(s)	298, 300
BssMI	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
BssNI	GRCGYC	1 cut(s)	69
BssT1I	CCWWGG	2 cut(s)	574, 642
Bst4CI	ACNGT	2 cut(s)	938, 1273
Bst6I	CTCTTC	1 cut(s)	1176
BstACI	GRCGYC	1 cut(s)	69
BstAPI	GCANNNNNTGC	2 cut(s)	561, 1030
BstBAI	YACGTR	1 cut(s)	1415
BstC8I	GCNNGC	6 cut(s)	57, 190, 300, 302, 429, 508
BstDEI	CTNAG	8 cut(s)	251, 618, 693, 801, 1005, 1299, 1372, 1559
BstDSI	CCRYGG	1 cut(s)	548
BstF5I	GGATG	4 cut(s)	652, 1221, 1395, 1483
BstFNI	CGCG	4 cut(s)	66, 300, 302, 304
BstH2I	RGCGCY	1 cut(s)	324
BstHHI	GCGC	6 cut(s)	66, 300, 302, 304, 323, 492
BstKTI	GATC	9 cut(s)	292, 464, 571, 588, 787, 1014, 1240, 1309, 1378
BstMAI	GTCTC	4 cut(s)	449, 850, 985, 1388
BstMBI	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
BstNSI	RCATGY	1 cut(s)	250
BstSCI	CCNGG	1 cut(s)	394
BstSFI	CTRYAG	2 cut(s)	76, 1034
BstSLI	GKGCMC	1 cut(s)	1370
BstUI	CGCG	4 cut(s)	66, 300, 302, 304
BstV1I	GCAGC	8 cut(s)	145, 307, 319, 322, 325, 330, 758, 1647
BstV2I	GAAGAC	1 cut(s)	906
BstX2I	RGATCY	2 cut(s)	1237, 1306
BstXI	CCANNNNNNTGG	2 cut(s)	740, 1617
BstYI	RGATCY	2 cut(s)	1237, 1306
Bsu15I	ATCGAT	1 cut(s)	1136
BsuI	GTATCC	1 cut(s)	720
BsuRI	GGCC	3 cut(s)	422, 476, 1130
BsuTUI	ATCGAT	1 cut(s)	1136
BtgI	CCRYGG	1 cut(s)	548
BtgZI	GCGATG	1 cut(s)	282
BtrI	CACGTC	2 cut(s)	350, 412
BtsCI	GGATG	4 cut(s)	652, 1221, 1395, 1483
BtsIMutI	CAGTG	2 cut(s)	1278, 1648
Cac8I	GCNNGC	6 cut(s)	57, 190, 300, 302, 429, 508
CaiI	CAGNNNCTG	1 cut(s)	1310
CciI	TCATGA	1 cut(s)	1378
CfoI	GCGC	6 cut(s)	66, 300, 302, 304, 323, 492
Cfr10I	RCCGGY	2 cut(s)	188, 469
Cfr13I	GGNCC	2 cut(s)	475, 628
ClaI	ATCGAT	1 cut(s)	1136
Csp6I	GTAC	3 cut(s)	1068, 1175, 1592
CspAI	ACCGGT	1 cut(s)	469
CviAII	CATG	6 cut(s)	247, 556, 1154, 1202, 1379, 1679
CviQI	GTAC	3 cut(s)	1068, 1175, 1592
DdeI	CTNAG	8 cut(s)	251, 618, 693, 801, 1005, 1299, 1372, 1559
DpnI	GATC	9 cut(s)	291, 463, 570, 587, 786, 1013, 1239, 1308, 1377
DpnII	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
DraI	TTTAAA	3 cut(s)	1210, 1326, 1464
DrdI	GACNNNNNNGTC	1 cut(s)	842
DseDI	GACNNNNNNGTC	1 cut(s)	842
Eam1104I	CTCTTC	1 cut(s)	1176
EarI	CTCTTC	1 cut(s)	1176
EciI	GGCGGA	1 cut(s)	402
Ecl136II	GAGCTC	1 cut(s)	31
Eco130I	CCWWGG	2 cut(s)	574, 642
Eco24I	GRGCYC	3 cut(s)	33, 396, 1426
Eco47I	GGWCC	1 cut(s)	628
Eco53kI	GAGCTC	1 cut(s)	31
Eco57I	CTGAAG	1 cut(s)	927
Eco88I	CYCGRG	1 cut(s)	357
EcoICRI	GAGCTC	1 cut(s)	31
EcoRI	GAATTC	1 cut(s)	1344
EcoT14I	CCWWGG	2 cut(s)	574, 642
EcoT22I	ATGCAT	3 cut(s)	566, 1203, 1680
EcoT38I	GRGCYC	3 cut(s)	33, 396, 1426
ErhI	CCWWGG	2 cut(s)	574, 642
FaeI	CATG	6 cut(s)	250, 559, 1157, 1205, 1382, 1682
FalI	AAGNNNNNCTT	6 cut(s)	1203, 1235, 1355, 1387, 1614, 1646
FaqI	GGGAC	2 cut(s)	395, 1511
FatI	CATG	6 cut(s)	246, 555, 1153, 1201, 1378, 1678
FauI	CCCGC	3 cut(s)	169, 204, 754
FblI	GTMKAC	3 cut(s)	243, 414, 1530
FokI	GGATG	4 cut(s)	659, 1228, 1402, 1490
FriOI	GRGCYC	3 cut(s)	33, 396, 1426
FspBI	CTAG	2 cut(s)	575, 1386
GlaI	GCGC	6 cut(s)	65, 299, 301, 303, 322, 491
GsaI	CCCAGC	1 cut(s)	64
GsuI	CTGGAG	3 cut(s)	20, 76, 1313
HaeII	RGCGCY	1 cut(s)	324
HaeIII	GGCC	3 cut(s)	422, 476, 1130
HapII	CCGG	3 cut(s)	189, 396, 470
HhaI	GCGC	6 cut(s)	66, 300, 302, 304, 323, 492
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	6 cut(s)	250, 559, 1157, 1205, 1382, 1682
Hin6I	GCGC	6 cut(s)	64, 298, 300, 302, 321, 490
HinP1I	GCGC	6 cut(s)	64, 298, 300, 302, 321, 490
HincII	GTYRAC	4 cut(s)	73, 244, 415, 1483
HindII	GTYRAC	4 cut(s)	73, 244, 415, 1483
HinfI	GANTC	9 cut(s)	233, 275, 620, 799, 875, 1301, 1450, 1469, 1517
HpaII	CCGG	3 cut(s)	189, 396, 470
HphI	GGTGA	1 cut(s)	1553
Hpy166II	GTNNAC	8 cut(s)	73, 220, 244, 415, 1175, 1368, 1483, 1531
Hpy188I	TCNGA	7 cut(s)	238, 628, 694, 1046, 1306, 1432, 1477
Hpy188III	TCNNGA	8 cut(s)	37, 403, 443, 671, 1262, 1379, 1454, 1618
Hpy8I	GTNNAC	8 cut(s)	73, 220, 244, 415, 1175, 1368, 1483, 1531
HpyAV	CCTTC	5 cut(s)	677, 902, 1120, 1158, 1517
HpyCH4III	ACNGT	2 cut(s)	938, 1273
HpyCH4IV	ACGT	7 cut(s)	69, 126, 349, 411, 602, 1171, 1414
HpyF3I	CTNAG	8 cut(s)	251, 618, 693, 801, 1005, 1299, 1372, 1559
HpySE526I	ACGT	7 cut(s)	69, 126, 349, 411, 602, 1171, 1414
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	6 cut(s)	250, 559, 1157, 1205, 1382, 1682
HspAI	GCGC	6 cut(s)	64, 298, 300, 302, 321, 490
KroI	GCCGGC	1 cut(s)	188
KroNI	GCCGGC	1 cut(s)	190
Kzo9I	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
LmnI	GCTCC	4 cut(s)	36, 52, 1288, 1294
Lsp1109I	GCAGC	8 cut(s)	145, 307, 319, 322, 325, 330, 758, 1647
LweI	GCATC	6 cut(s)	361, 694, 1188, 1206, 1423, 1543
MaeI	CTAG	2 cut(s)	575, 1386
MaeII	ACGT	7 cut(s)	69, 126, 349, 411, 602, 1171, 1414
MaeIII	GTNAC	1 cut(s)	1410
MalI	GATC	9 cut(s)	291, 463, 570, 587, 786, 1013, 1239, 1308, 1377
MauBI	CGCGCGCG	1 cut(s)	300
MboI	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
MboII	GAAGA	8 cut(s)	908, 911, 1021, 1193, 1247, 1464, 1469, 1556
MfeI	CAATTG	1 cut(s)	861
MflI	RGATCY	2 cut(s)	1237, 1306
MhlI	GDGCHC	5 cut(s)	33, 396, 769, 1370, 1426
MluCI	AATT	7 cut(s)	121, 532, 698, 829, 861, 1071, 1344
MlyI	GAGTC	5 cut(s)	269, 629, 793, 884, 1310
MmeI	TCCRAC	1 cut(s)	606
Mph1103I	ATGCAT	3 cut(s)	566, 1203, 1680
MroNI	GCCGGC	1 cut(s)	188
MspA1I	CMGCKG	2 cut(s)	264, 817
MspI	CCGG	3 cut(s)	189, 396, 470
MspR9I	CCNGG	1 cut(s)	396
MunI	CAATTG	1 cut(s)	861
Mva1269I	GAATGC	3 cut(s)	566, 971, 1035
MvnI	CGCG	4 cut(s)	66, 300, 302, 304
NaeI	GCCGGC	1 cut(s)	190
NciI	CCSGG	1 cut(s)	396
NdeII	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
NgoMIV	GCCGGC	1 cut(s)	188
NlaIII	CATG	6 cut(s)	250, 559, 1157, 1205, 1382, 1682
NlaIV	GGNNCC	2 cut(s)	1043, 1296
NmeAIII	GCCGAG	2 cut(s)	177, 448
NsiI	ATGCAT	3 cut(s)	566, 1203, 1680
NspI	RCATGY	1 cut(s)	250
PagI	TCATGA	1 cut(s)	1378
PauI	GCGCGC	2 cut(s)	298, 300
PcsI	WCGNNNNNNNCGW	6 cut(s)	18, 24, 228, 420, 439, 448
PctI	GAATGC	3 cut(s)	566, 971, 1035
PdiI	GCCGGC	1 cut(s)	190
PfeI	GAWTC	4 cut(s)	233, 1450, 1469, 1517
PinAI	ACCGGT	1 cut(s)	469
PleI	GAGTC	5 cut(s)	269, 628, 793, 883, 1309
PpsI	GAGTC	5 cut(s)	269, 628, 793, 883, 1309
Ppu21I	YACGTR	1 cut(s)	1415
Psp124BI	GAGCTC	1 cut(s)	33
Psp1406I	AACGTT	1 cut(s)	602
PspFI	CCCAGC	1 cut(s)	60
PspN4I	GGNNCC	2 cut(s)	1043, 1296
PspPI	GGNCC	2 cut(s)	475, 628
PstNI	CAGNNNCTG	1 cut(s)	1310
PsuI	RGATCY	2 cut(s)	1237, 1306
PteI	GCGCGC	2 cut(s)	298, 300
PvuII	CAGCTG	1 cut(s)	817
RsaI	GTAC	3 cut(s)	1069, 1176, 1593
RsaNI	GTAC	3 cut(s)	1068, 1175, 1592
SacI	GAGCTC	1 cut(s)	33
SalI	GTCGAC	2 cut(s)	242, 413
Sau3AI	GATC	9 cut(s)	289, 461, 568, 585, 784, 1011, 1237, 1306, 1375
Sau96I	GGNCC	2 cut(s)	475, 628
SchI	GAGTC	5 cut(s)	269, 629, 793, 884, 1310
ScrFI	CCNGG	1 cut(s)	396
SduI	GDGCHC	5 cut(s)	33, 396, 769, 1370, 1426
SfaNI	GCATC	6 cut(s)	361, 694, 1188, 1206, 1423, 1543
SfcI	CTRYAG	2 cut(s)	76, 1034
SgrAI	CRCCGGYG	1 cut(s)	469
SgrDI	CGTCGACG	1 cut(s)	413
SinI	GGWCC	1 cut(s)	628
SmiI	ATTTAAAT	2 cut(s)	1326, 1464
SmlI	CTYRAG	3 cut(s)	47, 999, 1358
SmoI	CTYRAG	3 cut(s)	47, 999, 1358
Sse9I	AATT	7 cut(s)	121, 532, 698, 829, 861, 1071, 1344
SspMI	CTAG	2 cut(s)	575, 1386
SstI	GAGCTC	1 cut(s)	33
StyD4I	CCNGG	1 cut(s)	394
StyI	CCWWGG	2 cut(s)	574, 642
SwaI	ATTTAAAT	2 cut(s)	1326, 1464
TaaI	ACNGT	2 cut(s)	938, 1273
TaiI	ACGT	7 cut(s)	72, 129, 352, 414, 605, 1174, 1417
TaqI	TCGA	7 cut(s)	5, 243, 310, 414, 500, 1136, 1348
TasI	AATT	7 cut(s)	121, 532, 698, 829, 861, 1071, 1344
TatI	WGTACW	3 cut(s)	1067, 1174, 1591
TauI	GCSGC	7 cut(s)	167, 211, 267, 318, 326, 344, 377
TfiI	GAWTC	4 cut(s)	233, 1450, 1469, 1517
TscAI	CASTG	2 cut(s)	1278, 1648
TseI	GCWGC	8 cut(s)	133, 295, 318, 332, 335, 338, 746, 1660
TspDTI	ATGAA	2 cut(s)	1395, 1482
TspGWI	ACGGA	4 cut(s)	627, 739, 811, 904
TspRI	CASTG	2 cut(s)	1278, 1648
VneI	GTGCAC	1 cut(s)	1366
VpaK11BI	GGWCC	1 cut(s)	628
XapI	RAATTY	2 cut(s)	698, 1344
XceI	RCATGY	1 cut(s)	250
XmaJI	CCTAGG	1 cut(s)	574
XmiI	GTMKAC	3 cut(s)	243, 414, 1530
XspI	CTAG	2 cut(s)	575, 1386
ZraI	GACGTC	1 cut(s)	70
Zsp2I	ATGCAT	3 cut(s)	566, 1203, 1680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.