Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0003765

Physical Location & Seq

Genomic Coordinates Reverse (-)

22064667 .. 22082663

Gene Structure 17997 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G009920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3270 bp

ATGTCGAGCGGCAGTGAGGACGAGGATGAGCTCCTCCAGATGGCTCTGAAGGAGCAATCTCAGAGAGATGTGAATTATCAGAAGCCACCGTCAAGCAACTCCCGGAGACCCGTTGCCAATTACGTCCAAGCCCCTCCTCCTCCTCCGGCAGCAGCCAAGAAGTCCAATGGCAACAAGAAGCCGCCGCAGCAGAAGCGGCGAGTGGTCGACGACGACGATGAATCGGATGTCGATATGCTCAGCATATCCAGCGGCGATGAGGACTCCACCTCGAGAGATCAGCAACGTGTGCGGTTCCGCGGCTCTTCTGGTGCCGCCAGACCTAAAGACGACGAGGCTGCTTGGGACGGCGACGAGCCCGGTTGCTGGAAGCATGTGGACGAGGCCGAGCTTGCTCGCAGGGTTCGTGGAATGCGAGAGACAAGAGCAGCACCGGTGGCCATTAAAGTTGAGCGCAAAGCGTCCACTGCAGGTTTGCCAAGGAAGGGACTAACCAATTTACAGTCCTTCCCTCGTGGCATGGAGTGTATTGATCCCCTAGGCCTCGGCATTATAGACAACAAAACCTTGAGACTCATAACCGAGTCCTCCGACTATTCTCCAACCAAGGATGACAAATTGGACAACACCCTTCGTGAGAAGTTGTTGTATTTCTCTGAGAAATTTGAGGCCAAGGTCTTCATATCCCGGATTCACCAAGAAACAAATGCAGCTGATTTGGAGGCTGGTGCTCTGGCTCTTAAAAGTGATCTTATAGGACGCACTCAGCAGAGAAAACAATTGGTCAAAGACAATTTTGACTGCTTCGTCTCTTGCAAAACCACAATTGATGATATCGAGTCAAAATTAAAACGGATTGAAGATGATCCTGAAGGTTCAGGGACTACTCATTTGTTCAAATGCATGGAAGGAGTCAGTTCATTGGCTAATCGTGCTTTCCAGCATCTATTTGAGAGACAGGCTGAAGCTGAAAAGATTAGGTCAGTCCAGGGAATGCTGCAGAGGTTCCGAACGCTATTCAATTTGCCCAGTACAATTCGTGGTAGCATAAGCAAGGGTGAATATGACTTGGCAGTTAGGGAATACAAGAAGGCGAAGTCGATTGCTCTCCCTTCTCATGTGGGAATACTGAAACGTGTTCTTGAAGAGGTTGAGAAGGTGATGCATGAGTTTAAAGGCACTCTATACAAGTCTATGGAAGATCCACAGATAGATCTAACCAATCTTGAAAACACAGTAAGACTACTGTTGGAGCTGGAGCCTGAGTCGGATCCTGTTTGGCATTATTTAAATATACAGAATAACAGAATTCGAGGTTTGCTTGAGAAGTGCACACTAGATCATGAAGCTAGAATGGAGACTCTACATAATGTTTTACGTGAAAGGGCTTTATTTGATGCAAGATGGAGGCAGATTCAACAAGATACCAATCAATCTTCAGATGCTCTTGCTTTTGGAAACAACAGTCTTCTGGTTGATTCTGTCGCAGTCGATTTGACTGGTGAAGAAGTTGATGCTCTTAGAGGGAGGTATATTCGTAGATTAACTGCTGTGCTTACACATCACATACCAGCATTCTGGAAAGTAGCACTTTCTGTTTTCAGCGGAAAATTCGCAAAGTCATCCCAAGTTTCTACGGAATCAAATGCTAATACTCCAGCAAATAAATCTGAGGAAAAAGTTGGAGATGGGAAATACTCTACTCATTCTCTTGATGAAGTTTCCGTAATGATACGCAATACTATAGCAGCATATGAAGTGAAGGTTTGCAATACCTTTCGTGATCTTGAAGAATCAAATATTCTGCAACCATATATGAGTGATGCAATAATTGAAATATCCAAAGCCTGTGAAGCCTTTGAAGCGAAGGAATCAGCTCCATCTATTGCTGTAATTGCAACTCGAGCACTTCAGTCAGAGATTACAAAGATCTATATTTTAAGGCTTTGCTCATGGATGCGGGCATCAACGGTAGAGATTTCAAAAGATGAGGCATGGGTCCCAGTATCAGTTCTTGAACGGAATAAATCTCCCTATACAATATCTTACTTGCCACTAGTGTTCCGTAATGTTATGACCTCAGCAATGGATCAGATCAAGTTGATGATTCAGACATTAAGGAGTGAGGCGACCAGGTCAGAGGATATGTTTGTGCAACTCCAAGAAATACAAGAATCTGTCAGACTTGCATTTTTGAATTGTATTTTGGATTTTGCAGGTCATCTGGAGCGTATAGGTAGTGAGCTTACTCAAAACAGATCAAGCAAAGGAAGTTCACATGTAGAAAATGGATATTCACAGAACCTAGAAGAGAAGCAAATGTTTGATCTCAGGGGAAGTGTTGTGGGTCCCCATCAACAGTTGTTGATAGTTTTGAGTAATATTGGCTATTGCAAAGAAGAGCTTTCTTATGAATTGTACAACAACTACAAGCATATCTGGCTACAGTCGAGGGAAAGAGAGGAAGAGGATAGTGACATACAAGATTTAGTGATGTCTTTTTCTGGGCTGGAAGAGAAGGTTCTTGAACAGTATACTTTTGCTAAGGCAAATTTGATCAGAACGGCTGCTTCAAACTATTTGTTAGATTCTGGTGTTCAATGGGGAGCAGCACCTGCTGTAAAAGGTGTGCGTGATGCTGCTGTGGAGTTGCTGCACACTTTGGTAGCTGTACATGCAGAGGTTTTTTCTGGTGCTAAGCCTTTTCTGGACAAGACACTTGGCATTCTTGTAGAAGGTCTAATTGATACTTTTATCAGCCTGTTCCACGAAAACAGCATGAAAGAGCTTAGGTCACTTGACACAAATGGTTTCTGCCAGCTAATGCTTGAGCTTGAATATTTCGAGACCATTTTAAATCCATATTTTACACCTGATGCAAGAGAGGCTCTGAAGTCTTTACAAGGGGTGCTTCTGAATAAAGCAACTGAAAGTGTGACTGAAAATGTGGAGAATCCAGGACACAACCGGCGAGCAACACGTGGAAGTGAAGATGCAGTGCCAGATGATAAACTGCCAGGGATGACTGTGTCACCAGATGATCTGATTGCTCATGCCCAGCAATATAGCTCTGAGTTACTTCAAGCAGAGCTTGAGAGGACTCACATCAATACAGCATGCTTTGTTGAGTCAATTCCTTTGGACTTAGCACCAGAATCAGCCAAACGCGCTTATGCTTCCTTTAGGGGGTCACTGGATTCTCCCAGTAGAAACTATAGAGGCACACAAGGAGCAGCATCTCCAAGCTACTCCAGGAATAGGCGTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000145 GO:0001927 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0005938 GO:0006810 GO:0006887 GO:0006904 GO:0007154 GO:0008037 GO:0008150 GO:0009506 GO:0009856 GO:0009875 GO:0009987 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0022414 GO:0022607 GO:0030054 GO:0031982 GO:0032501 GO:0032940 GO:0032991 GO:0034622 GO:0043226 GO:0043227 GO:0043230 GO:0043933 GO:0044085 GO:0044421 GO:0044424 GO:0044444 GO:0044448 GO:0044464 GO:0044706 GO:0046903 GO:0048278 GO:0048544 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051704 GO:0055044 GO:0060321 GO:0065003 GO:0070062 GO:0071840 GO:0071944 GO:0099023 GO:0099568 GO:0140029 GO:0140056 GO:1903561

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

Sec5

Protein Analysis

1089

Amino Acids

122.45

Weight (kDa)

5.55

Isoelectric Point (pI)

52.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec5	PF15469	178 - 1041	2.6e-143	Exocyst complex component Sec5
Dor1	PF04124	244 - 442	1.8e-07	Dor1-like family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21170 AT1G21170 AT1G76850
fragaria_vesca	FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280
malus_domestica	MD02G1241200.v1.1 MD02G1241400.v1.1 MD07G1075500.v1.1
prunus_persica	Prupe.8G058700_v2.0.a1 Prupe.8G058700_v2.0.a1
pyrus_communis	pycom02g20640 pycom02g20660 pycom02g20670 pycom02g20680 pycom02g20750 pycom02g20760 pycom07g05950
rosa_chinensis	RchiOBHm_Chr1g0332001
rosa_laevigata	RLG00000029699
rosa_multiflora	Rmu_co8474765.1_g000001 Rmu_sc0003358.1_g000014
rosa_roxburghii	Rroxscaffold_4G00318510
rosa_rugosa	Rorug01G0097700
rosa_samantha	Rh1AG121600 Rh1BG093100 Rh1CG116400 Rh1DG127300
rosa_wichuraiana	Rw1G009920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2623
AasI	GACNNNNNNGTC	1 cut(s)	806
Acc36I	ACCTGC	3 cut(s)	461, 2207, 2623
AccB1I	GGYRCC	1 cut(s)	311
AccBSI	CCGCTC	1 cut(s)	9
AccI	GTMKAC	2 cut(s)	207, 2534
AccII	CGCG	2 cut(s)	300, 3168
AclWI	GGATC	6 cut(s)	527, 860, 1196, 1265, 1278, 2097
AcoI	YGGCCR	1 cut(s)	438
AcsI	RAATTY	4 cut(s)	662, 1308, 1610, 2551
AcuI	CTGAAG	6 cut(s)	68, 891, 984, 1422, 1895, 2912
AcvI	CACGTG	1 cut(s)	2981
AcyI	GRCGYC	1 cut(s)	3262
AfaI	GTAC	3 cut(s)	1033, 2420, 2673
AfiI	CCNNNNNNNGG	5 cut(s)	40, 366, 485, 3186, 3258
AflIII	ACRYGT	4 cut(s)	286, 1135, 2278, 2978
AgeI	ACCGGT	1 cut(s)	433
AhlI	ACTAGT	1 cut(s)	2056
AjnI	CCWGG	5 cut(s)	987, 2132, 2956, 3016, 3251
AjuI	GAANNNNNNNTTGG	2 cut(s)	2189, 2221
Alw21I	GWGCWC	4 cut(s)	33, 733, 1334, 1909
Alw26I	GTCTC	7 cut(s)	100, 413, 565, 814, 949, 1352, 2840
Alw44I	GTGCAC	1 cut(s)	1330
AlwI	GGATC	6 cut(s)	527, 860, 1196, 1265, 1278, 2097
AlwNI	CAGNNNCTG	1 cut(s)	2615
Ama87I	CYCGRG	2 cut(s)	271, 1902
AoxI	GGCC	4 cut(s)	384, 438, 541, 669
ApaLI	GTGCAC	1 cut(s)	1330
ApoI	RAATTY	4 cut(s)	662, 1308, 1610, 2551
ArsI	GACNNNNNNTTYG	2 cut(s)	874, 906
AsiGI	ACCGGT	1 cut(s)	433
AspA2I	CCTAGG	1 cut(s)	538
AspLEI	GCGC	2 cut(s)	456, 3170
AspS9I	GGNCC	2 cut(s)	1999, 2348
AsuC2I	CCSGG	3 cut(s)	103, 360, 688
AsuHPI	GGTGA	5 cut(s)	686, 1070, 1171, 1514, 3024
AvaI	CYCGRG	2 cut(s)	271, 1902
AvaII	GGWCC	2 cut(s)	1999, 2348
AvrII	CCTAGG	1 cut(s)	538
BaeGI	GKGCMC	1 cut(s)	1334
BalI	TGGCCA	1 cut(s)	440
BamHI	GGATCC	1 cut(s)	1270
BanI	GGYRCC	1 cut(s)	311
BanII	GRGCYC	2 cut(s)	33, 360
BauI	CACGAG	1 cut(s)	513
BbrPI	CACGTG	1 cut(s)	2981
BbsI	GAAGAC	2 cut(s)	670, 1460
Bbv12I	GWGCWC	4 cut(s)	33, 733, 1334, 1909
BbvCI	CCTCAGC	1 cut(s)	2080
BccI	CCATC	5 cut(s)	34, 1398, 1682, 1888, 2361
BceAI	ACGGC	2 cut(s)	364, 2580
BcgI	CGANNNNNNTGC	2 cut(s)	320, 354
BciT130I	CCWGG	5 cut(s)	989, 2134, 2958, 3018, 3253
BclI	TGATCA	1 cut(s)	2556
BcnI	CCSGG	3 cut(s)	103, 360, 688
BcoDI	GTCTC	7 cut(s)	100, 413, 565, 814, 949, 1352, 2840
BcuI	ACTAGT	1 cut(s)	2056
BfaI	CTAG	5 cut(s)	539, 1337, 1350, 2057, 2306
BfmI	CTRYAG	5 cut(s)	468, 998, 1743, 2444, 3214
BfuAI	ACCTGC	3 cut(s)	461, 2207, 2623
BglII	AGATCT	2 cut(s)	1213, 1929
BlnI	CCTAGG	1 cut(s)	538
BlpI	GCTNAGC	2 cut(s)	239, 2697
Bme1390I	CCNGG	8 cut(s)	103, 360, 688, 989, 2134, 2958, 3018, 3253
Bme18I	GGWCC	2 cut(s)	1999, 2348
BmeT110I	CYCGRG	2 cut(s)	271, 1902
BmgT120I	GGNCC	2 cut(s)	1999, 2348
BmiI	GGNNCC	9 cut(s)	296, 313, 1007, 1260, 1272, 2000, 2001, 2349, 2350
BmrFI	CCNGG	8 cut(s)	103, 360, 688, 989, 2134, 2958, 3018, 3253
BmrI	ACTGGG	3 cut(s)	1023, 1997, 3198
BmuI	ACTGGG	3 cut(s)	1023, 1997, 3198
BpiI	GAAGAC	2 cut(s)	670, 1460
BpmI	CTGGAG	5 cut(s)	20, 1277, 1641, 2246, 3235
Bpu10I	CCTNAGC	3 cut(s)	2080, 2544, 2789
Bpu1102I	GCTNAGC	2 cut(s)	239, 2697
BpuEI	CTTGAG	4 cut(s)	589, 1343, 2849, 3113
BpuMI	CCSGG	3 cut(s)	103, 360, 688
BsaAI	YACGTR	2 cut(s)	1379, 2981
BsaBI	GATNNNNATC	1 cut(s)	1428
BsaHI	GRCGYC	1 cut(s)	3262
BsaI	GGTCTC	2 cut(s)	100, 2840
BsaJI	CCNNGG	8 cut(s)	298, 479, 538, 544, 606, 672, 988, 3017
BsaWI	WCCGGW	1 cut(s)	433
BsaXI	ACNNNNNCTCC	2 cut(s)	1250, 1280
Bsc4I	CCNNNNNNNGG	5 cut(s)	40, 366, 485, 3186, 3258
Bse118I	RCCGGY	2 cut(s)	433, 2967
Bse1I	ACTGG	5 cut(s)	1029, 1504, 2003, 3198, 3204
Bse3DI	GCAATG	1 cut(s)	2091
Bse8I	GATNNNNATC	1 cut(s)	1428
BseBI	CCWGG	5 cut(s)	989, 2134, 2958, 3018, 3253
BseDI	CCNNGG	8 cut(s)	298, 479, 538, 544, 606, 672, 988, 3017
BseGI	GGATG	6 cut(s)	31, 232, 616, 1622, 1962, 3027
BseJI	GATNNNNATC	1 cut(s)	1428
BseLI	CCNNNNNNNGG	5 cut(s)	40, 366, 485, 3186, 3258
BseMI	GCAATG	1 cut(s)	2091
BseMII	CTCAG	9 cut(s)	74, 253, 648, 779, 1254, 1662, 2094, 2344, 3063
BseNI	ACTGG	5 cut(s)	1029, 1504, 2003, 3198, 3204
BseRI	GAGGAG	4 cut(s)	23, 126, 129, 132
BseSI	GKGCMC	1 cut(s)	1334
BseYI	CCCAGC	1 cut(s)	3057
BsgI	GTGCAG	1 cut(s)	2639
Bsh1236I	CGCG	2 cut(s)	300, 3168
BshFI	GGCC	4 cut(s)	386, 440, 543, 671
BshNI	GGYRCC	1 cut(s)	311
BshTI	ACCGGT	1 cut(s)	433
BsiHKAI	GWGCWC	4 cut(s)	33, 733, 1334, 1909
BsiHKCI	CYCGRG	2 cut(s)	271, 1902
BsiSI	CCGG	6 cut(s)	103, 146, 360, 434, 688, 2968
BslFI	GGGAC	5 cut(s)	359, 501, 895, 1985, 2334
BslI	CCNNNNNNNGG	5 cut(s)	40, 366, 485, 3186, 3258
BsmAI	GTCTC	7 cut(s)	100, 413, 565, 814, 949, 1352, 2840
BsmBI	CGTCTC	1 cut(s)	814
BsmFI	GGGAC	5 cut(s)	359, 501, 895, 1985, 2334
BsmI	GAATGC	4 cut(s)	417, 999, 1574, 2724
BsnI	GGCC	4 cut(s)	386, 440, 543, 671
Bso31I	GGTCTC	2 cut(s)	100, 2840
BsoBI	CYCGRG	2 cut(s)	271, 1902
Bsp1286I	GDGCHC	5 cut(s)	33, 360, 733, 1334, 1909
Bsp1407I	TGTACA	2 cut(s)	2418, 2671
Bsp1720I	GCTNAGC	2 cut(s)	239, 2697
BspANI	GGCC	4 cut(s)	386, 440, 543, 671
BspCNI	CTCAG	9 cut(s)	73, 252, 649, 778, 1255, 1663, 2093, 2343, 3064
BspFNI	CGCG	2 cut(s)	300, 3168
BspHI	TCATGA	1 cut(s)	1342
BspLI	GGNNCC	9 cut(s)	296, 313, 1007, 1260, 1272, 2000, 2001, 2349, 2350
BspMAI	CTGCAG	2 cut(s)	472, 1002
BspMI	ACCTGC	3 cut(s)	461, 2207, 2623
BspPI	GGATC	6 cut(s)	527, 860, 1196, 1265, 1278, 2097
BspQI	GCTCTTC	2 cut(s)	310, 2394
BspT107I	GGYRCC	1 cut(s)	311
BspTNI	GGTCTC	2 cut(s)	100, 2840
BsrBI	CCGCTC	1 cut(s)	9
BsrDI	GCAATG	1 cut(s)	2091
BsrFI	RCCGGY	2 cut(s)	433, 2967
BsrGI	TGTACA	2 cut(s)	2418, 2671
BsrI	ACTGG	5 cut(s)	1029, 1504, 2003, 3198, 3204
BssAI	RCCGGY	2 cut(s)	433, 2967
BssECI	CCNNGG	8 cut(s)	298, 479, 538, 544, 606, 672, 988, 3017
BssNAI	GTATAC	1 cut(s)	2535
BssNI	GRCGYC	1 cut(s)	3262
BssSI	CACGAG	1 cut(s)	513
BssT1I	CCWWGG	4 cut(s)	479, 538, 606, 672
Bst1107I	GTATAC	1 cut(s)	2535
Bst2BI	CACGAG	1 cut(s)	513
Bst2UI	CCWGG	5 cut(s)	989, 2134, 2958, 3018, 3253
Bst6I	CTCTTC	6 cut(s)	310, 1140, 2304, 2394, 2460, 2508
BstACI	GRCGYC	1 cut(s)	3262
BstAPI	GCANNNNNTGC	2 cut(s)	289, 2615
BstAUI	TGTACA	2 cut(s)	2418, 2671
BstBAI	YACGTR	2 cut(s)	1379, 2981
BstC8I	GCNNGC	6 cut(s)	393, 397, 1962, 2819, 2973, 3118
BstDSI	CCRYGG	1 cut(s)	298
BstF5I	GGATG	6 cut(s)	31, 232, 616, 1622, 1962, 3027
BstFNI	CGCG	2 cut(s)	300, 3168
BstHHI	GCGC	2 cut(s)	456, 3170
BstMAI	GTCTC	7 cut(s)	100, 413, 565, 814, 949, 1352, 2840
BstNI	CCWGG	5 cut(s)	989, 2134, 2958, 3018, 3253
BstNSI	RCATGY	4 cut(s)	377, 2282, 2678, 3120
BstSCI	CCNGG	8 cut(s)	101, 358, 686, 987, 2132, 2956, 3016, 3251
BstSFI	CTRYAG	5 cut(s)	468, 998, 1743, 2444, 3214
BstSLI	GKGCMC	1 cut(s)	1334
BstUI	CGCG	2 cut(s)	300, 3168
BstV2I	GAAGAC	2 cut(s)	670, 1460
BstX2I	RGATCY	4 cut(s)	1201, 1213, 1270, 1929
BstXI	CCANNNNNNTGG	1 cut(s)	1578
BstYI	RGATCY	4 cut(s)	1201, 1213, 1270, 1929
BstZ17I	GTATAC	1 cut(s)	2535
BsuRI	GGCC	4 cut(s)	386, 440, 543, 671
BtgI	CCRYGG	1 cut(s)	298
BtgZI	GCGATG	1 cut(s)	270
BtsCI	GGATG	6 cut(s)	31, 232, 616, 1622, 1962, 3027
BtsI	GCAGTG	3 cut(s)	19, 465, 3003
BtsIMutI	CAGTG	4 cut(s)	19, 465, 3003, 3191
BveI	ACCTGC	3 cut(s)	461, 2207, 2623
Cac8I	GCNNGC	6 cut(s)	393, 397, 1962, 2819, 2973, 3118
CaiI	CAGNNNCTG	1 cut(s)	2615
CciI	TCATGA	1 cut(s)	1342
CfoI	GCGC	2 cut(s)	456, 3170
Cfr10I	RCCGGY	2 cut(s)	433, 2967
Cfr13I	GGNCC	2 cut(s)	1999, 2348
Cfr42I	CCGCGG	1 cut(s)	301
CseI	GACGC	3 cut(s)	450, 768, 3251
CsiI	ACCWGGT	1 cut(s)	2132
Csp6I	GTAC	3 cut(s)	1032, 2419, 2672
CspAI	ACCGGT	1 cut(s)	433
CviQI	GTAC	3 cut(s)	1032, 2419, 2672
DraI	TTTAAA	3 cut(s)	1174, 1290, 2856
DrdI	GACNNNNNNGTC	1 cut(s)	806
DseDI	GACNNNNNNGTC	1 cut(s)	806
EaeI	YGGCCR	1 cut(s)	438
Eam1104I	CTCTTC	6 cut(s)	310, 1140, 2304, 2394, 2460, 2508
EarI	CTCTTC	6 cut(s)	310, 1140, 2304, 2394, 2460, 2508
Ecl136II	GAGCTC	1 cut(s)	31
Eco130I	CCWWGG	4 cut(s)	479, 538, 606, 672
Eco147I	AGGCCT	1 cut(s)	543
Eco24I	GRGCYC	2 cut(s)	33, 360
Eco31I	GGTCTC	2 cut(s)	100, 2840
Eco32I	GATATC	1 cut(s)	835
Eco47I	GGWCC	2 cut(s)	1999, 2348
Eco53kI	GAGCTC	1 cut(s)	31
Eco57I	CTGAAG	6 cut(s)	68, 891, 984, 1422, 1895, 2912
Eco72I	CACGTG	1 cut(s)	2981
Eco88I	CYCGRG	2 cut(s)	271, 1902
EcoICRI	GAGCTC	1 cut(s)	31
EcoO109I	RGGNCCY	2 cut(s)	1999, 2348
EcoRI	GAATTC	1 cut(s)	1308
EcoRII	CCWGG	5 cut(s)	987, 2132, 2956, 3016, 3251
EcoRV	GATATC	1 cut(s)	835
EcoT14I	CCWWGG	4 cut(s)	479, 538, 606, 672
EcoT22I	ATGCAT	2 cut(s)	905, 1167
EcoT38I	GRGCYC	2 cut(s)	33, 360
ErhI	CCWWGG	4 cut(s)	479, 538, 606, 672
Esp3I	CGTCTC	1 cut(s)	814
FalI	AAGNNNNNCTT	8 cut(s)	1575, 1607, 2388, 2420, 2895, 2927, 3075, 3107
FaqI	GGGAC	5 cut(s)	359, 501, 895, 1985, 2334
FauI	CCCGC	1 cut(s)	1953
FauNDI	CATATG	1 cut(s)	1753
FbaI	TGATCA	1 cut(s)	2556
FblI	GTMKAC	2 cut(s)	207, 2534
FokI	GGATG	6 cut(s)	38, 239, 623, 1609, 1969, 3034
FriOI	GRGCYC	2 cut(s)	33, 360
FspBI	CTAG	5 cut(s)	539, 1337, 1350, 2057, 2306
GlaI	GCGC	2 cut(s)	455, 3169
GsaI	CCCAGC	1 cut(s)	3061
GsuI	CTGGAG	5 cut(s)	20, 1277, 1641, 2246, 3235
HaeIII	GGCC	4 cut(s)	386, 440, 543, 671
HapII	CCGG	6 cut(s)	103, 146, 360, 434, 688, 2968
HgaI	GACGC	3 cut(s)	450, 768, 3251
HhaI	GCGC	2 cut(s)	456, 3170
Hin1I	GRCGYC	1 cut(s)	3262
Hin6I	GCGC	2 cut(s)	454, 3168
HinP1I	GCGC	2 cut(s)	454, 3168
HincII	GTYRAC	1 cut(s)	208
HindII	GTYRAC	1 cut(s)	208
HpaII	CCGG	6 cut(s)	103, 146, 360, 434, 688, 2968
HphI	GGTGA	5 cut(s)	686, 1070, 1171, 1514, 3024
Hpy166II	GTNNAC	6 cut(s)	208, 379, 465, 1332, 2276, 2535
Hpy8I	GTNNAC	6 cut(s)	208, 379, 465, 1332, 2276, 2535
Hpy99I	CGWCG	6 cut(s)	212, 215, 218, 335, 356, 3267
HpyCH4IV	ACGT	5 cut(s)	123, 286, 1135, 1378, 2980
HpySE526I	ACGT	5 cut(s)	123, 286, 1135, 1378, 2980
Hsp92I	GRCGYC	1 cut(s)	3262
HspAI	GCGC	2 cut(s)	454, 3168
KflI	GGGWCCC	2 cut(s)	1999, 2348
Ksp22I	TGATCA	1 cut(s)	2556
KspI	CCGCGG	1 cut(s)	301
LguI	GCTCTTC	2 cut(s)	310, 2394
LmnI	GCTCC	8 cut(s)	36, 52, 1252, 1258, 1882, 2227, 2606, 3230
MabI	ACCWGGT	1 cut(s)	2132
MaeI	CTAG	5 cut(s)	539, 1337, 1350, 2057, 2306
MaeII	ACGT	5 cut(s)	123, 286, 1135, 1378, 2980
MaeIII	GTNAC	6 cut(s)	2474, 2793, 2935, 3030, 3075, 3189
MbiI	CCGCTC	1 cut(s)	9
MfeI	CAATTG	2 cut(s)	779, 825
MflI	RGATCY	4 cut(s)	1201, 1213, 1270, 1929
MhlI	GDGCHC	5 cut(s)	33, 360, 733, 1334, 1909
MlsI	TGGCCA	1 cut(s)	440
MluNI	TGGCCA	1 cut(s)	440
MlyI	GAGTC	9 cut(s)	257, 567, 593, 848, 921, 1274, 1354, 3094, 3137
MmeI	TCCRAC	5 cut(s)	615, 626, 1230, 1248, 1663
Mox20I	TGGCCA	1 cut(s)	440
Mph1103I	ATGCAT	2 cut(s)	905, 1167
MscI	TGGCCA	1 cut(s)	440
MseI	TTAA	9 cut(s)	444, 741, 848, 1173, 1289, 1544, 1940, 2117, 2855
Msp20I	TGGCCA	1 cut(s)	440
MspA1I	CMGCKG	4 cut(s)	252, 300, 713, 1605
MspI	CCGG	6 cut(s)	103, 146, 360, 434, 688, 2968
MspR9I	CCNGG	8 cut(s)	103, 360, 688, 989, 2134, 2958, 3018, 3253
MunI	CAATTG	2 cut(s)	779, 825
Mva1269I	GAATGC	4 cut(s)	417, 999, 1574, 2724
MvaI	CCWGG	5 cut(s)	989, 2134, 2958, 3018, 3253
MvnI	CGCG	2 cut(s)	300, 3168
NciI	CCSGG	3 cut(s)	103, 360, 688
NdeI	CATATG	1 cut(s)	1753
NlaIV	GGNNCC	9 cut(s)	296, 313, 1007, 1260, 1272, 2000, 2001, 2349, 2350
NmeAIII	GCCGAG	2 cut(s)	412, 525
NmuCI	GTSAC	5 cut(s)	2474, 2793, 2935, 3030, 3189
NsiI	ATGCAT	2 cut(s)	905, 1167
NspI	RCATGY	4 cut(s)	377, 2282, 2678, 3120
PaeI	GCATGC	1 cut(s)	3120
PaeR7I	CTCGAG	2 cut(s)	271, 1902
PagI	TCATGA	1 cut(s)	1342
PaqCI	CACCTGC	1 cut(s)	2623
PceI	AGGCCT	1 cut(s)	543
PciI	ACATGT	1 cut(s)	2278
PciSI	GCTCTTC	2 cut(s)	310, 2394
PcsI	WCGNNNNNNNCGW	3 cut(s)	213, 403, 412
PctI	GAATGC	4 cut(s)	417, 999, 1574, 2724
PflFI	GACNNNGTC	2 cut(s)	2134, 3028
PfoI	TCCNGGA	4 cut(s)	101, 686, 2956, 3251
PinAI	ACCGGT	1 cut(s)	433
PleI	GAGTC	9 cut(s)	257, 567, 592, 847, 920, 1273, 1354, 3094, 3136
PmaCI	CACGTG	1 cut(s)	2981
PmlI	CACGTG	1 cut(s)	2981
PpsI	GAGTC	9 cut(s)	257, 567, 592, 847, 920, 1273, 1354, 3094, 3136
Ppu21I	YACGTR	2 cut(s)	1379, 2981
PpuMI	RGGWCCY	2 cut(s)	1999, 2348
PscI	ACATGT	1 cut(s)	2278
Psp124BI	GAGCTC	1 cut(s)	33
Psp5II	RGGWCCY	2 cut(s)	1999, 2348
Psp6I	CCWGG	5 cut(s)	987, 2132, 2956, 3016, 3251
PspCI	CACGTG	1 cut(s)	2981
PspFI	CCCAGC	1 cut(s)	3057
PspGI	CCWGG	5 cut(s)	987, 2132, 2956, 3016, 3251
PspN4I	GGNNCC	9 cut(s)	296, 313, 1007, 1260, 1272, 2000, 2001, 2349, 2350
PspPI	GGNCC	2 cut(s)	1999, 2348
PspPPI	RGGWCCY	2 cut(s)	1999, 2348
PspXI	VCTCGAGB	1 cut(s)	1902
PstI	CTGCAG	2 cut(s)	472, 1002
PstNI	CAGNNNCTG	1 cut(s)	2615
PsuI	RGATCY	4 cut(s)	1201, 1213, 1270, 1929
PsyI	GACNNNGTC	2 cut(s)	2134, 3028
PvuII	CAGCTG	1 cut(s)	713
RsaI	GTAC	3 cut(s)	1033, 2420, 2673
RsaNI	GTAC	3 cut(s)	1032, 2419, 2672
SacI	GAGCTC	1 cut(s)	33
SacII	CCGCGG	1 cut(s)	301
SalI	GTCGAC	1 cut(s)	206
SapI	GCTCTTC	2 cut(s)	310, 2394
SaqAI	TTAA	9 cut(s)	444, 741, 848, 1173, 1289, 1544, 1940, 2117, 2855
Sau96I	GGNCC	2 cut(s)	1999, 2348
SchI	GAGTC	9 cut(s)	257, 567, 593, 848, 921, 1274, 1354, 3094, 3137
ScrFI	CCNGG	8 cut(s)	103, 360, 688, 989, 2134, 2958, 3018, 3253
SduI	GDGCHC	5 cut(s)	33, 360, 733, 1334, 1909
SexAI	ACCWGGT	1 cut(s)	2132
SfcI	CTRYAG	5 cut(s)	468, 998, 1743, 2444, 3214
Sfr274I	CTCGAG	2 cut(s)	271, 1902
Sfr303I	CCGCGG	1 cut(s)	301
SgrAI	CRCCGGYG	1 cut(s)	433
SgrBI	CCGCGG	1 cut(s)	301
SinI	GGWCC	2 cut(s)	1999, 2348
SlaI	CTCGAG	2 cut(s)	271, 1902
SmiI	ATTTAAAT	1 cut(s)	1290
SmlI	CTYRAG	6 cut(s)	271, 568, 1322, 1902, 2828, 3092
SmoI	CTYRAG	6 cut(s)	271, 568, 1322, 1902, 2828, 3092
SpeI	ACTAGT	1 cut(s)	2056
SphI	GCATGC	1 cut(s)	3120
SseBI	AGGCCT	1 cut(s)	543
SspI	AATATT	3 cut(s)	1801, 2383, 2840
SspMI	CTAG	5 cut(s)	539, 1337, 1350, 2057, 2306
SstI	GAGCTC	1 cut(s)	33
StuI	AGGCCT	1 cut(s)	543
StyD4I	CCNGG	8 cut(s)	101, 358, 686, 987, 2132, 2956, 3016, 3251
StyI	CCWWGG	4 cut(s)	479, 538, 606, 672
SwaI	ATTTAAAT	1 cut(s)	1290
TaiI	ACGT	5 cut(s)	126, 289, 1138, 1381, 2983
TatI	WGTACW	3 cut(s)	1031, 2418, 2671
TauI	GCSGC	7 cut(s)	12, 184, 187, 199, 255, 303, 317
Tru1I	TTAA	9 cut(s)	444, 741, 848, 1173, 1289, 1544, 1940, 2117, 2855
Tru9I	TTAA	9 cut(s)	444, 741, 848, 1173, 1289, 1544, 1940, 2117, 2855
TscAI	CASTG	4 cut(s)	19, 472, 3003, 3198
TseFI	GTSAC	5 cut(s)	2474, 2793, 2935, 3030, 3189
Tsp45I	GTSAC	5 cut(s)	2474, 2793, 2935, 3030, 3189
TspDTI	ATGAA	8 cut(s)	234, 670, 909, 1359, 1731, 1770, 2427, 2795
TspGWI	ACGGA	5 cut(s)	868, 1652, 1714, 2035, 2054
TspRI	CASTG	4 cut(s)	19, 472, 3003, 3198
Tth111I	GACNNNGTC	2 cut(s)	2134, 3028
VneI	GTGCAC	1 cut(s)	1330
VpaK11BI	GGWCC	2 cut(s)	1999, 2348
XapI	RAATTY	4 cut(s)	662, 1308, 1610, 2551
XceI	RCATGY	4 cut(s)	377, 2282, 2678, 3120
XhoI	CTCGAG	2 cut(s)	271, 1902
XmaJI	CCTAGG	1 cut(s)	538
XmiI	GTMKAC	2 cut(s)	207, 2534
XspI	CTAG	5 cut(s)	539, 1337, 1350, 2057, 2306
Zsp2I	ATGCAT	2 cut(s)	905, 1167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.