Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0003765

Physical Location & Seq

Genomic Coordinates Reverse (-)

7219685 .. 7232312

Gene Structure 12628 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1075500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3303 bp

ATGTCGAGCGACGGCGACGACCTGGACGAGGACGAGCTCCTCCAGATGGCTCTGAAGGAACAATCCCAGCGCGACGTCAACTACAAAAAACCCACCTCCAGCAACTCCCGCTCTGCCCCCGTCGCCAACTACGTCCAGCATCCCCACCAACAACCGCCGCGTAAACCCGCCGCTTCTCCTGCCCCCAACCACGCTACCAGAGCCAGCAACACCGGCAACAGGCGGCGGGCGGTTGAGGAGGACGACGAATCCGATGTCGACATGCTCAGCATATCCAGCGGCGATGAGGACTCCACCGCCAAAGATCAGCAGCGCGCGCGGTTCCGAGGAGGCAAGGCTGCTTCTGCTGCTGCCTCCAGACGTGGTTCTCGAGGAGATGATGATGCCGCTTGGGATGGCGGAGAGCCCGGTTGCTGGAAGCACGTCGACGAGGCTGAGCTTGCTCGTAGGGTTCGTGAAATGCGAGAGACAAGATCAGCACCAGTGGCCCAAAAGGTTGAGCGCAAAGTTTCGACAGCGGGCTTGGCGCGAAAGGGGCTTAACAATTTACAGTCTTTCCCCCGGGGCATGGAATGCATTGATCCCCTAGGCTTGGGGATCATAGACAACAAAACCTTAAGACTGATTACTGAGTCGTCCGACCATTCTCCGTCCAAGGGTGACAAACTGGATAACAACCTTCGTGAGAAGTTGTTGTATTTCTCTGAGAAATTTGATGCCAAGCTCTTTATCTCACGGATACACCAAGATACTGGTGCAGCAGATTTGGAGGCTGGTGCTCTTGCTCTAAAAAGTGATCTTAAAGGACGAACTCAGCAGAGAAAACAATTGGTCAAAGACAATTTTGACTGCTTCGTCTCGTGCAAAACGACAATTGATGATATTGAGTCAAAGTTAAAGCGGATTGAAGAAGATCCTGAAGGTTCAGGCACATCTCATTTGTTTAACTGTATGCAAGGAGTCAGTTCGTTGGCTAATCGTGCATTCCAGCCTCTATTTGAGAGACAGGCTCAAGCTGAGAAGATCAGGTCCGTACAAGGAATGCTACAGAGGTTCCGAACGCTCTTCAACTTGCCCAGTACAATTCGTGGTAGCATAAGCAAGGGAGAATATGACTTGGCAGTTAGGGAATACAAGAAGGCCAAATCGATTGCTCTGCCTTCTCATGTGGGAATACTGAAACGGGTACTTGAAGAGGTTGAGAAAGTGATGCATGAGTTTAAAGGGATGCTTTACAAGTCTATGGAAGATCCACAAATAGATTTAACAAATGTTGAAAACACAGTGAGGCTGTTATTGGAGCTGGAACCTGAGTCAGATCCTGTATGGCACTATTTAAATATACAGAATAACAGAATTCGAGGTTTGCTTGAGAAATGCACATTGGATCATGAAGCTAGGATGGGGACTTTACATAATGAGTTACGTGAAAGGGCTTTGTCTGATGCAAGATGGAGACAGATTCAAGAAGATATAAGTCAATCTTCATATGTCAACTATTCTCTTGGGGACAACCACCTTCCAGTAGATTCTCTGCCAGTGGACTTGACAGGTGAAGAAGTTGATGCACTTAGAGGGAGGTATATTCGCAGATTAACTGCTGTACTTATCCATCACATACCAGCTTTCTGGAAAGTGGCACTTTCTGTTTTCAGTGGGAAATTCGCAAAGTCGTCCCAAGTTTCTACTGAATCAAATGCTAACGCTCCAGCAAATAAAGCTGAGGATAAAGTTGGAGATGGGAAGTACTCCACTCATTCCCTTGATGAAGTTGCTGGAATGATACGGAATACCATATCTGCGTATGAAGTCAAGGTTTGCAATACATTTCGCGATCTTGAAGAATCAAACATTCTGCAACCGTATATGAGGGATGCCATAACAGAAATATCTAGAGCATGTGAAGCTTTCCAAGCAAAAGAATCAGCTCCTTCTATTGCTGTAACTGCAACTCGAGCACTTCAGTCGGAAATTACTAAGATTTACATCCTAAGGCTCTGCTCATGGATGCGGGCATCAACTGCAGAGATTTCAAAAGACGAGACATGGGTTCCAGTATCGGTTCTTGAAAGGAATAAATCTCCCTATACAATCTCTTTCTTGCCTCTAGCATTCTGTAATGTTATGACCTCAGCAATGGATCAAATCAAGTTGATGATTCAATCTTTAAGAACTGAGGCTACGAAGTCAGAGGATATGTTCATGCAACTCCAAGAAACACAAGAATCTGTCAGACTTGCATTTTTGAACTGTATGCTTGATTTTGCAGGTCATCTAGAGCGTAGTGGAAGTGAGCTTGCCATAAACAAATCAAGCAAAGGAAGTTCACACGTACAAAATGGATATTCTCATACCCTAGAAGAAAAGTCAGATCTCCCAGGAAGTGTTAGTCCACATCAACAGTTGTTAATAGTCTTGAGTAATGTTGGCTATTGCAGAGAAGAACTTTCTTATGAGTTGTACAACAATTACAAGCACATCTGGCTACAGTCTAGGGAAAGAGGAGAAGAGGATGGTGACATACAAGACCTAGTGGTGTCCTTTTCTGGCCTTGAAGAGAAGGTCCTTGAGCAATATACTTTTGCTAAGGCAAATTTGATCAGAACTGCTGCCTGGAACTATTTATTGGATTCTGGTGTTCAATGGGGAGCAGCACCTGCTGTAAAAGGTGTGCGTGATGCTGCTGTGGAGTTACTGCACACTTTGGTAGCTGTGCATGCAGAGGTATTTTCTGGTGCTAAGCCCTTATTGGACAAGACACTTGGCATTCTTGTGGAAGGTCTAATTGACACTTTTATCAGTCTGTTCCATGAAAATAAATCGAAAGAGCTTAGGTCACTTGATGCAAATGGTTTCTGTCAGCTAATGCTAGAGCTTGAATACTTCGAGACTATATTGAATCCATATTTCACTGCGGATGCAAGGGAGTCTATGAAGTCCTTACAAGGGGTACTTCTCGATAAAGCCACTGAGAGTGTATCAGAAAATGCGGAGAATCCAGGGCATAATCGACGAGCAACACGTGGAAGTGAAGAGGCTGCTGATGATAGACAGGATGGGATGAGTGTATCACCAGATGACCTCATTGCACTTGCTCAGCAGTATAGCTCTGAATTACTTCAAGCAGAGCTTGAGAGGACTCACATCAACACGGCATGTTTTGTAGAGTCAATTCCGTTAGACTCAGTACCCGAATCAGCTAAACGTGCTTATGCTTCGTTTAGGGGATCACTAGATTCTCCCAGCAGGAACTACAGAGGCAACACAGGATCCCCAAGCTACACTCGGAATAGGCGTCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000145 GO:0001927 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0005938 GO:0006810 GO:0006887 GO:0006904 GO:0007154 GO:0008037 GO:0008150 GO:0009506 GO:0009856 GO:0009875 GO:0009987 GO:0016020 GO:0016043 GO:0016192 GO:0022406 GO:0022414 GO:0022607 GO:0030054 GO:0031982 GO:0032501 GO:0032940 GO:0032991 GO:0034622 GO:0043226 GO:0043227 GO:0043230 GO:0043933 GO:0044085 GO:0044421 GO:0044424 GO:0044444 GO:0044448 GO:0044464 GO:0044706 GO:0046903 GO:0048278 GO:0048544 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051704 GO:0055044 GO:0060321 GO:0065003 GO:0070062 GO:0071840 GO:0071944 GO:0099023 GO:0099568 GO:0140029 GO:0140056 GO:1903561

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

Sec5

Protein Analysis

1101

Amino Acids

123.04

Weight (kDa)

5.71

Isoelectric Point (pI)

52.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sec5	PF15469	194 - 1054	1.1e-147	Exocyst complex component Sec5
Dor1	PF04124	260 - 432	7.2e-07	Dor1-like family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21170 AT1G21170 AT1G76850
fragaria_vesca	FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280 FvH4_7g05280
malus_domestica	MD02G1241200.v1.1 MD02G1241400.v1.1 MD07G1075500.v1.1
prunus_persica	Prupe.8G058700_v2.0.a1 Prupe.8G058700_v2.0.a1
pyrus_communis	pycom02g20640 pycom02g20660 pycom02g20670 pycom02g20680 pycom02g20750 pycom02g20760 pycom07g05950
rosa_chinensis	RchiOBHm_Chr1g0332001
rosa_laevigata	RLG00000029699
rosa_multiflora	Rmu_co8474765.1_g000001 Rmu_sc0003358.1_g000014
rosa_roxburghii	Rroxscaffold_4G00318510
rosa_rugosa	Rorug01G0097700
rosa_samantha	Rh1AG121600 Rh1BG093100 Rh1CG116400 Rh1DG127300
rosa_wichuraiana	Rw1G009920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2665
AasI	GACNNNNNNGTC	1 cut(s)	854
AatII	GACGTC	1 cut(s)	78
Acc36I	ACCTGC	2 cut(s)	2258, 2665
AccBSI	CCGCTC	1 cut(s)	111
AccI	GTMKAC	2 cut(s)	258, 426
AccII	CGCG	7 cut(s)	72, 160, 315, 317, 319, 529, 1833
AcsI	RAATTY	4 cut(s)	710, 1356, 1661, 2593
AcuI	CTGAAG	3 cut(s)	74, 939, 1946
AcvI	CACGTG	1 cut(s)	3023
AcyI	GRCGYC	2 cut(s)	75, 3295
AfaI	GTAC	9 cut(s)	1035, 1081, 1188, 1605, 1748, 2334, 2462, 2952, 3189
AfiI	CCNNNNNNNGG	8 cut(s)	28, 46, 219, 414, 568, 592, 656, 2946
AflII	CTTAAG	1 cut(s)	616
AflIII	ACRYGT	2 cut(s)	2329, 3020
AjiI	CACGTC	2 cut(s)	362, 424
AjnI	CCWGG	4 cut(s)	21, 2377, 2612, 2998
AjuI	GAANNNNNNNTTGG	4 cut(s)	1367, 1399, 2609, 2641
Alw21I	GWGCWC	3 cut(s)	39, 781, 1960
Alw26I	GTCTC	6 cut(s)	461, 862, 997, 1450, 2036, 2882
AlwNI	CAGNNNCTG	2 cut(s)	1322, 2657
Ama87I	CYCGRG	3 cut(s)	369, 561, 1953
AoxI	GGCC	3 cut(s)	486, 1140, 2548
ApeKI	GCWGC	9 cut(s)	310, 338, 347, 350, 758, 2609, 2651, 2681, 3038
ApoI	RAATTY	4 cut(s)	710, 1356, 1661, 2593
ArsI	GACNNNNNNTTYG	2 cut(s)	240, 272
Asp700I	GAANNNNTTC	1 cut(s)	3117
AspA2I	CCTAGG	1 cut(s)	586
AspLEI	GCGC	6 cut(s)	72, 315, 317, 319, 504, 529
AspS9I	GGNCC	3 cut(s)	487, 1029, 2563
AsuC2I	CCSGG	3 cut(s)	408, 562, 563
AsuHPI	GGTGA	4 cut(s)	671, 1565, 2528, 3063
AvaI	CYCGRG	3 cut(s)	369, 561, 1953
AvaII	GGWCC	2 cut(s)	1029, 2563
AvrII	CCTAGG	1 cut(s)	586
AxyI	CCTNAGG	1 cut(s)	1991
BamHI	GGATCC	1 cut(s)	3269
BanII	GRGCYC	2 cut(s)	39, 408
BauI	CACGAG	1 cut(s)	859
BbrPI	CACGTG	1 cut(s)	3023
Bbv12I	GWGCWC	3 cut(s)	39, 781, 1960
BbvCI	CCTCAGC	2 cut(s)	1722, 2131
BbvI	GCAGC	9 cut(s)	322, 325, 334, 337, 770, 2596, 2663, 2668, 3025
BccI	CCATC	8 cut(s)	40, 389, 1396, 1446, 1620, 1733, 2507, 3050
BceAI	ACGGC	2 cut(s)	28, 3168
BcgI	CGANNNNNNTGC	4 cut(s)	1138, 1172, 1947, 1981
BciT130I	CCWGG	4 cut(s)	23, 2379, 2614, 3000
BciVI	GTATCC	1 cut(s)	732
BclI	TGATCA	1 cut(s)	2598
BcnI	CCSGG	3 cut(s)	408, 562, 563
BcoDI	GTCTC	6 cut(s)	461, 862, 997, 1450, 2036, 2882
BfmI	CTRYAG	4 cut(s)	1046, 2022, 2486, 3253
BfrI	CTTAAG	1 cut(s)	616
BfuAI	ACCTGC	2 cut(s)	2258, 2665
BfuI	GTATCC	1 cut(s)	732
BglII	AGATCT	1 cut(s)	2371
BlnI	CCTAGG	1 cut(s)	586
BlpI	GCTNAGC	4 cut(s)	266, 435, 2739, 3096
BmcAI	AGTACT	1 cut(s)	1748
Bme1390I	CCNGG	7 cut(s)	23, 408, 562, 563, 2379, 2614, 3000
Bme18I	GGWCC	2 cut(s)	1029, 2563
BmeT110I	CYCGRG	3 cut(s)	369, 561, 1953
BmgBI	CACGTC	2 cut(s)	362, 424
BmgT120I	GGNCC	3 cut(s)	487, 1029, 2563
BmiI	GGNNCC	5 cut(s)	323, 1055, 1308, 2052, 3271
BmrFI	CCNGG	7 cut(s)	23, 408, 562, 563, 2379, 2614, 3000
BmrI	ACTGGG	1 cut(s)	1071
BmuI	ACTGGG	1 cut(s)	1071
BplI	GAGNNNNNCTC	2 cut(s)	994, 1026
BpmI	CTGGAG	4 cut(s)	26, 82, 340, 1692
Bpu10I	CCTNAGC	4 cut(s)	1722, 2131, 2586, 2831
Bpu1102I	GCTNAGC	4 cut(s)	266, 435, 2739, 3096
BpuEI	CTTGAG	5 cut(s)	996, 1391, 2437, 2588, 3152
BpuMI	CCSGG	3 cut(s)	408, 562, 563
Bsa29I	ATCGAT	1 cut(s)	1148
BsaAI	YACGTR	3 cut(s)	1427, 2332, 3023
BsaBI	GATNNNNATC	1 cut(s)	1985
BsaHI	GRCGYC	2 cut(s)	75, 3295
BsaJI	CCNNGG	8 cut(s)	325, 560, 561, 562, 586, 654, 2377, 2999
Bsc4I	CCNNNNNNNGG	8 cut(s)	28, 46, 219, 414, 568, 592, 656, 2946
Bse118I	RCCGGY	1 cut(s)	212
Bse1I	ACTGG	7 cut(s)	482, 672, 757, 1077, 1523, 1538, 2054
Bse21I	CCTNAGG	1 cut(s)	1991
Bse3DI	GCAATG	2 cut(s)	2142, 3084
Bse8I	GATNNNNATC	1 cut(s)	1985
BseBI	CCWGG	4 cut(s)	23, 2379, 2614, 3000
BseCI	ATCGAT	1 cut(s)	1148
BseDI	CCNNGG	8 cut(s)	325, 560, 561, 562, 586, 654, 2377, 2999
BseJI	GATNNNNATC	1 cut(s)	1985
BseLI	CCNNNNNNNGG	8 cut(s)	28, 46, 219, 414, 568, 592, 656, 2946
BseMI	GCAATG	2 cut(s)	2142, 3084
BseNI	ACTGG	7 cut(s)	482, 672, 757, 1077, 1523, 1538, 2054
BsePI	GCGCGC	2 cut(s)	313, 315
BseRI	GAGGAG	5 cut(s)	29, 251, 342, 387, 2517
BseXI	GCAGC	9 cut(s)	322, 325, 334, 337, 770, 2596, 2663, 2668, 3025
BseYI	CCCAGC	2 cut(s)	66, 3242
BsgI	GTGCAG	2 cut(s)	777, 2681
Bsh1236I	CGCG	7 cut(s)	72, 160, 315, 317, 319, 529, 1833
BshFI	GGCC	3 cut(s)	488, 1142, 2550
BshVI	ATCGAT	1 cut(s)	1148
BsiHKAI	GWGCWC	3 cut(s)	39, 781, 1960
BsiHKCI	CYCGRG	3 cut(s)	369, 561, 1953
BsiSI	CCGG	3 cut(s)	213, 408, 562
BslFI	GGGAC	3 cut(s)	1420, 1523, 1660
BslI	CCNNNNNNNGG	8 cut(s)	28, 46, 219, 414, 568, 592, 656, 2946
BsmAI	GTCTC	6 cut(s)	461, 862, 997, 1450, 2036, 2882
BsmBI	CGTCTC	1 cut(s)	862
BsmFI	GGGAC	3 cut(s)	1420, 1523, 1660
BsmI	GAATGC	5 cut(s)	578, 983, 1047, 2111, 2766
BsnI	GGCC	3 cut(s)	488, 1142, 2550
BsoBI	CYCGRG	3 cut(s)	369, 561, 1953
Bsp1286I	GDGCHC	4 cut(s)	39, 408, 781, 1960
Bsp1407I	TGTACA	1 cut(s)	2460
Bsp1720I	GCTNAGC	4 cut(s)	266, 435, 2739, 3096
Bsp68I	TCGCGA	1 cut(s)	1833
BspANI	GGCC	3 cut(s)	488, 1142, 2550
BspDI	ATCGAT	1 cut(s)	1148
BspFNI	CGCG	7 cut(s)	72, 160, 315, 317, 319, 529, 1833
BspHI	TCATGA	1 cut(s)	1390
BspLI	GGNNCC	5 cut(s)	323, 1055, 1308, 2052, 3271
BspMAI	CTGCAG	1 cut(s)	2026
BspMI	ACCTGC	2 cut(s)	2258, 2665
BspQI	GCTCTTC	1 cut(s)	1070
BspTI	CTTAAG	1 cut(s)	616
BsrBI	CCGCTC	1 cut(s)	111
BsrDI	GCAATG	2 cut(s)	2142, 3084
BsrFI	RCCGGY	1 cut(s)	212
BsrGI	TGTACA	1 cut(s)	2460
BsrI	ACTGG	7 cut(s)	482, 672, 757, 1077, 1523, 1538, 2054
BssAI	RCCGGY	1 cut(s)	212
BssECI	CCNNGG	8 cut(s)	325, 560, 561, 562, 586, 654, 2377, 2999
BssHII	GCGCGC	2 cut(s)	313, 315
BssNI	GRCGYC	2 cut(s)	75, 3295
BssSI	CACGAG	1 cut(s)	859
BssT1I	CCWWGG	2 cut(s)	586, 654
Bst2BI	CACGAG	1 cut(s)	859
Bst2UI	CCWGG	4 cut(s)	23, 2379, 2614, 3000
Bst4CI	ACNGT	7 cut(s)	552, 950, 1285, 1863, 2252, 2403, 2490
Bst6I	CTCTTC	5 cut(s)	1070, 1188, 2502, 2550, 3027
BstACI	GRCGYC	2 cut(s)	75, 3295
BstAFI	CTTAAG	1 cut(s)	616
BstAPI	GCANNNNNTGC	3 cut(s)	573, 2021, 2657
BstAUI	TGTACA	1 cut(s)	2460
BstBAI	YACGTR	3 cut(s)	1427, 2332, 3023
BstC8I	GCNNGC	9 cut(s)	205, 228, 315, 317, 441, 520, 2013, 2298, 2718
BstENI	CCTNNNNNAGG	1 cut(s)	26
BstFNI	CGCG	7 cut(s)	72, 160, 315, 317, 319, 529, 1833
BstHHI	GCGC	6 cut(s)	72, 315, 317, 319, 504, 529
BstMAI	GTCTC	6 cut(s)	461, 862, 997, 1450, 2036, 2882
BstNI	CCWGG	4 cut(s)	23, 2379, 2614, 3000
BstNSI	RCATGY	4 cut(s)	265, 1902, 2720, 3159
BstSCI	CCNGG	7 cut(s)	21, 406, 560, 561, 2377, 2612, 2998
BstSFI	CTRYAG	4 cut(s)	1046, 2022, 2486, 3253
BstUI	CGCG	7 cut(s)	72, 160, 315, 317, 319, 529, 1833
BstV1I	GCAGC	9 cut(s)	322, 325, 334, 337, 770, 2596, 2663, 2668, 3025
BstX2I	RGATCY	5 cut(s)	913, 1249, 1318, 2371, 3269
BstXI	CCANNNNNNTGG	2 cut(s)	752, 1629
BstYI	RGATCY	5 cut(s)	913, 1249, 1318, 2371, 3269
Bsu15I	ATCGAT	1 cut(s)	1148
Bsu36I	CCTNAGG	1 cut(s)	1991
BsuI	GTATCC	1 cut(s)	732
BsuRI	GGCC	3 cut(s)	488, 1142, 2550
BsuTUI	ATCGAT	1 cut(s)	1148
BtgZI	GCGATG	1 cut(s)	297
BtrI	CACGTC	2 cut(s)	362, 424
BtsI	GCAGTG	1 cut(s)	2910
BtsIMutI	CAGTG	6 cut(s)	489, 1290, 1545, 1660, 2910, 2967
BtuMI	TCGCGA	1 cut(s)	1833
BveI	ACCTGC	2 cut(s)	2258, 2665
Cac8I	GCNNGC	9 cut(s)	205, 228, 315, 317, 441, 520, 2013, 2298, 2718
CaiI	CAGNNNCTG	2 cut(s)	1322, 2657
CciI	TCATGA	1 cut(s)	1390
CfoI	GCGC	6 cut(s)	72, 315, 317, 319, 504, 529
Cfr10I	RCCGGY	1 cut(s)	212
Cfr13I	GGNCC	3 cut(s)	487, 1029, 2563
Cfr9I	CCCGGG	1 cut(s)	561
ClaI	ATCGAT	1 cut(s)	1148
CseI	GACGC	1 cut(s)	3284
Csp6I	GTAC	9 cut(s)	1034, 1080, 1187, 1604, 1747, 2333, 2461, 2951, 3188
CviQI	GTAC	9 cut(s)	1034, 1080, 1187, 1604, 1747, 2333, 2461, 2951, 3188
DraI	TTTAAA	2 cut(s)	1222, 1338
DrdI	GACNNNNNNGTC	1 cut(s)	854
DseDI	GACNNNNNNGTC	1 cut(s)	854
Eam1104I	CTCTTC	5 cut(s)	1070, 1188, 2502, 2550, 3027
EarI	CTCTTC	5 cut(s)	1070, 1188, 2502, 2550, 3027
EciI	GGCGGA	1 cut(s)	414
Ecl136II	GAGCTC	1 cut(s)	37
Eco130I	CCWWGG	2 cut(s)	586, 654
Eco24I	GRGCYC	2 cut(s)	39, 408
Eco47I	GGWCC	2 cut(s)	1029, 2563
Eco53kI	GAGCTC	1 cut(s)	37
Eco57I	CTGAAG	3 cut(s)	74, 939, 1946
Eco72I	CACGTG	1 cut(s)	3023
Eco81I	CCTNAGG	1 cut(s)	1991
Eco88I	CYCGRG	3 cut(s)	369, 561, 1953
EcoICRI	GAGCTC	1 cut(s)	37
EcoNI	CCTNNNNNAGG	1 cut(s)	26
EcoO109I	RGGNCCY	1 cut(s)	2563
EcoRI	GAATTC	1 cut(s)	1356
EcoRII	CCWGG	4 cut(s)	21, 2377, 2612, 2998
EcoT14I	CCWWGG	2 cut(s)	586, 654
EcoT22I	ATGCAT	2 cut(s)	578, 1215
EcoT38I	GRGCYC	2 cut(s)	39, 408
ErhI	CCWWGG	2 cut(s)	586, 654
Esp3I	CGTCTC	1 cut(s)	862
FaqI	GGGAC	3 cut(s)	1420, 1523, 1660
FauI	CCCGC	5 cut(s)	116, 175, 219, 511, 2004
FauNDI	CATATG	1 cut(s)	1489
FbaI	TGATCA	1 cut(s)	2598
FblI	GTMKAC	2 cut(s)	258, 426
FriOI	GRGCYC	2 cut(s)	39, 408
GlaI	GCGC	6 cut(s)	71, 314, 316, 318, 503, 528
GsaI	CCCAGC	2 cut(s)	70, 3246
GsuI	CTGGAG	4 cut(s)	26, 82, 340, 1692
HaeIII	GGCC	3 cut(s)	488, 1142, 2550
HapII	CCGG	3 cut(s)	213, 408, 562
HgaI	GACGC	1 cut(s)	3284
HhaI	GCGC	6 cut(s)	72, 315, 317, 319, 504, 529
Hin1I	GRCGYC	2 cut(s)	75, 3295
Hin6I	GCGC	6 cut(s)	70, 313, 315, 317, 502, 527
HinP1I	GCGC	6 cut(s)	70, 313, 315, 317, 502, 527
HincII	GTYRAC	4 cut(s)	79, 259, 427, 1495
HindII	GTYRAC	4 cut(s)	79, 259, 427, 1495
HindIII	AAGCTT	1 cut(s)	1905
HpaII	CCGG	3 cut(s)	213, 408, 562
HphI	GGTGA	4 cut(s)	671, 1565, 2528, 3063
Hpy166II	GTNNAC	8 cut(s)	79, 164, 259, 427, 1495, 1543, 2327, 2393
Hpy8I	GTNNAC	8 cut(s)	79, 164, 259, 427, 1495, 1543, 2327, 2393
Hpy99I	CGWCG	9 cut(s)	14, 20, 77, 125, 248, 428, 431, 3015, 3300
HpyAV	CCTTC	9 cut(s)	49, 689, 914, 1132, 1170, 1529, 1941, 2554, 2771
HpyCH4III	ACNGT	7 cut(s)	552, 950, 1285, 1863, 2252, 2403, 2490
HpyCH4IV	ACGT	8 cut(s)	75, 132, 361, 423, 1426, 2331, 3022, 3205
HpySE526I	ACGT	8 cut(s)	75, 132, 361, 423, 1426, 2331, 3022, 3205
Hsp92I	GRCGYC	2 cut(s)	75, 3295
HspAI	GCGC	6 cut(s)	70, 313, 315, 317, 502, 527
Ksp22I	TGATCA	1 cut(s)	2598
LguI	GCTCTTC	1 cut(s)	1070
LmnI	GCTCC	5 cut(s)	42, 1300, 1711, 1933, 2648
Lsp1109I	GCAGC	9 cut(s)	322, 325, 334, 337, 770, 2596, 2663, 2668, 3025
MaeII	ACGT	8 cut(s)	75, 132, 361, 423, 1426, 2331, 3022, 3205
MaeIII	GTNAC	6 cut(s)	659, 1422, 1942, 2516, 2691, 2835
MauBI	CGCGCGCG	1 cut(s)	315
MbiI	CCGCTC	1 cut(s)	111
MfeI	CAATTG	2 cut(s)	827, 873
MflI	RGATCY	5 cut(s)	913, 1249, 1318, 2371, 3269
MhlI	GDGCHC	4 cut(s)	39, 408, 781, 1960
MlyI	GAGTC	9 cut(s)	284, 641, 896, 969, 1322, 2936, 3133, 3176, 3176
MmeI	TCCRAC	3 cut(s)	663, 1714, 1947
Mph1103I	ATGCAT	2 cut(s)	578, 1215
MroXI	GAANNNNTTC	1 cut(s)	3117
MslI	CAYNNNNRTG	1 cut(s)	2771
MspA1I	CMGCKG	2 cut(s)	279, 518
MspCI	CTTAAG	1 cut(s)	616
MspI	CCGG	3 cut(s)	213, 408, 562
MspR9I	CCNGG	7 cut(s)	23, 408, 562, 563, 2379, 2614, 3000
MunI	CAATTG	2 cut(s)	827, 873
Mva1269I	GAATGC	5 cut(s)	578, 983, 1047, 2111, 2766
MvaI	CCWGG	4 cut(s)	23, 2379, 2614, 3000
MvnI	CGCG	7 cut(s)	72, 160, 315, 317, 319, 529, 1833
NciI	CCSGG	3 cut(s)	408, 562, 563
NdeI	CATATG	1 cut(s)	1489
NlaIV	GGNNCC	5 cut(s)	323, 1055, 1308, 2052, 3271
NmuCI	GTSAC	3 cut(s)	659, 2516, 2835
NruI	TCGCGA	1 cut(s)	1833
NsiI	ATGCAT	2 cut(s)	578, 1215
NspI	RCATGY	4 cut(s)	265, 1902, 2720, 3159
PaeI	GCATGC	1 cut(s)	2720
PaeR7I	CTCGAG	2 cut(s)	369, 1953
PagI	TCATGA	1 cut(s)	1390
PaqCI	CACCTGC	1 cut(s)	2665
PauI	GCGCGC	2 cut(s)	313, 315
PciSI	GCTCTTC	1 cut(s)	1070
PcsI	WCGNNNNNNNCGW	9 cut(s)	24, 129, 249, 367, 451, 460, 866, 3019, 3292
PctI	GAATGC	5 cut(s)	578, 983, 1047, 2111, 2766
PdmI	GAANNNNTTC	1 cut(s)	3117
PleI	GAGTC	9 cut(s)	284, 640, 895, 968, 1321, 2935, 3133, 3175, 3176
PmaCI	CACGTG	1 cut(s)	3023
PmlI	CACGTG	1 cut(s)	3023
PpsI	GAGTC	9 cut(s)	284, 640, 895, 968, 1321, 2935, 3133, 3175, 3176
Ppu21I	YACGTR	3 cut(s)	1427, 2332, 3023
PpuMI	RGGWCCY	1 cut(s)	2563
Psp124BI	GAGCTC	1 cut(s)	39
Psp5II	RGGWCCY	1 cut(s)	2563
Psp6I	CCWGG	4 cut(s)	21, 2377, 2612, 2998
PspCI	CACGTG	1 cut(s)	3023
PspFI	CCCAGC	2 cut(s)	66, 3242
PspGI	CCWGG	4 cut(s)	21, 2377, 2612, 2998
PspN4I	GGNNCC	5 cut(s)	323, 1055, 1308, 2052, 3271
PspPI	GGNCC	3 cut(s)	487, 1029, 2563
PspPPI	RGGWCCY	1 cut(s)	2563
PspXI	VCTCGAGB	1 cut(s)	1953
PsrI	GAACNNNNNNTAC	2 cut(s)	2164, 2196
PstI	CTGCAG	1 cut(s)	2026
PstNI	CAGNNNCTG	2 cut(s)	1322, 2657
PsuI	RGATCY	5 cut(s)	913, 1249, 1318, 2371, 3269
PteI	GCGCGC	2 cut(s)	313, 315
RruI	TCGCGA	1 cut(s)	1833
RsaI	GTAC	9 cut(s)	1035, 1081, 1188, 1605, 1748, 2334, 2462, 2952, 3189
RsaNI	GTAC	9 cut(s)	1034, 1080, 1187, 1604, 1747, 2333, 2461, 2951, 3188
RseI	CAYNNNNRTG	1 cut(s)	2771
SacI	GAGCTC	1 cut(s)	39
SalI	GTCGAC	2 cut(s)	257, 425
SapI	GCTCTTC	1 cut(s)	1070
Sau96I	GGNCC	3 cut(s)	487, 1029, 2563
ScaI	AGTACT	1 cut(s)	1748
SchI	GAGTC	9 cut(s)	284, 641, 896, 969, 1322, 2936, 3133, 3176, 3176
ScrFI	CCNGG	7 cut(s)	23, 408, 562, 563, 2379, 2614, 3000
SduI	GDGCHC	4 cut(s)	39, 408, 781, 1960
SfcI	CTRYAG	4 cut(s)	1046, 2022, 2486, 3253
Sfr274I	CTCGAG	2 cut(s)	369, 1953
SgrDI	CGTCGACG	1 cut(s)	425
SinI	GGWCC	2 cut(s)	1029, 2563
SlaI	CTCGAG	2 cut(s)	369, 1953
SmaI	CCCGGG	1 cut(s)	563
SmiI	ATTTAAAT	1 cut(s)	1338
SmiMI	CAYNNNNRTG	1 cut(s)	2771
SmlI	CTYRAG	8 cut(s)	369, 616, 1011, 1370, 1953, 2416, 2567, 3131
SmoI	CTYRAG	8 cut(s)	369, 616, 1011, 1370, 1953, 2416, 2567, 3131
SphI	GCATGC	1 cut(s)	2720
SstI	GAGCTC	1 cut(s)	39
StyD4I	CCNGG	7 cut(s)	21, 406, 560, 561, 2377, 2612, 2998
StyI	CCWWGG	2 cut(s)	586, 654
SwaI	ATTTAAAT	1 cut(s)	1338
TaaI	ACNGT	7 cut(s)	552, 950, 1285, 1863, 2252, 2403, 2490
TaiI	ACGT	8 cut(s)	78, 135, 364, 426, 1429, 2334, 3025, 3208
TatI	WGTACW	4 cut(s)	1079, 1603, 1746, 2460
TauI	GCSGC	5 cut(s)	160, 173, 226, 282, 389
TscAI	CASTG	6 cut(s)	489, 1290, 1545, 1660, 2917, 2974
TseFI	GTSAC	3 cut(s)	659, 2516, 2835
TseI	GCWGC	9 cut(s)	310, 338, 347, 350, 758, 2609, 2651, 2681, 3038
Tsp45I	GTSAC	3 cut(s)	659, 2516, 2835
TspDTI	ATGAA	7 cut(s)	1407, 1476, 1782, 1821, 2191, 2826, 2948
TspGWI	ACGGA	5 cut(s)	639, 751, 1021, 1801, 3165
TspMI	CCCGGG	1 cut(s)	561
TspRI	CASTG	6 cut(s)	489, 1290, 1545, 1660, 2917, 2974
Vha464I	CTTAAG	1 cut(s)	616
VpaK11BI	GGWCC	2 cut(s)	1029, 2563
XagI	CCTNNNNNAGG	1 cut(s)	26
XapI	RAATTY	4 cut(s)	710, 1356, 1661, 2593
XbaI	TCTAGA	2 cut(s)	1892, 2275
XceI	RCATGY	4 cut(s)	265, 1902, 2720, 3159
XhoI	CTCGAG	2 cut(s)	369, 1953
XmaI	CCCGGG	1 cut(s)	561
XmaJI	CCTAGG	1 cut(s)	586
XmiI	GTMKAC	2 cut(s)	258, 426
XmnI	GAANNNNTTC	1 cut(s)	3117
ZraI	GACGTC	1 cut(s)	76
ZrmI	AGTACT	1 cut(s)	1748
Zsp2I	ATGCAT	2 cut(s)	578, 1215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.