Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003086

Physical Location & Seq

Genomic Coordinates Reverse (-)

29969496 .. 29973925

Gene Structure 4430 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1248900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 885 bp

ATGGACCAGTACGTGAAAGTTGAGAAGATTGGTGAAGGAACTTATGGAGTGGTTTACAAGGCACGTGATCGTGTCACTAATGAGACTATAGCTTTGAAGAAAATTCGCTTGGAGCAGGAAGATGAGGGAGTGCCAAGCACTGCTATACGTGAGATTTCCCTCTTGAAAGAAATGCAGCATGGGAACATTGTCAGGTTACAGGATGTAGTGCACAGTGAGAAGCGCTTGTATCTGGTTTTTGAGTACCTGGACTTGGATCTGAAGAAGCATATGGATTCTACTCCTGAATTTGCAAAGGACCCTCGCCAAATAAAACTGTTTCTTTACCAGATTCTCAGAGGCATTGCTTACTGTCATTCACATAGAGTTCTTCATAGAGATCTTAAACCCCAGAATCTGCTGATAGATCGTCGTACCAATGCACTAAAGCTTGCAGATTTTGGACTGGCTAGAGCATTTGGTATTCCTGTTAGGACATTTACTCATGAGGTGGTGACCCTCTGGTACAGAGCGCCAGAGATATTGCTTGGTTCTCGCCATTACTCCACTCCAGTTGACGTGTGGTCAGTGGGTTGTATATTTGCCGAGATGGTAAACCAACGGCCTTTATTTCCTGGGGACTCTGAGATTGATGAATTGTTCAAGATATTTAGAATCATGGGTACTCCGACCGAGGATACATGGCCTGGAGTGAATTCTTTGCCTGATTTTAAATCTTCCTTTCCGAAGTGGGGTGCTAAGGAGTTGGCAAGTGTAGTTCCAAATCTTGATTCAGCTGGTGTTGATCTTCTCTCAAAAATGCTCTGCTTGGATCCCAGCAAAAGGATTACGGCCAGGAGTGCTCTTGAGCATGAGTACTTCAAAGATATTGCGTTTGTACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000278 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005819 GO:0005856 GO:0006139 GO:0006259 GO:0006281 GO:0006325 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016569 GO:0016570 GO:0016572 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0022402 GO:0030154 GO:0032502 GO:0033554 GO:0034641 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044770 GO:0044772 GO:0046483 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051276 GO:0051445 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0072686 GO:0090304 GO:0097472 GO:0140096 GO:1901360 GO:1901564 GO:1903047 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04068 ko04110 ko04114 ko04115 ko04151 ko04218 ko04914 ko04934 ko05161 ko05162 ko05165 ko05168 ko05169 ko05200 ko05203 ko05215 ko05222 ko05226 map04068 map04110 map04114 map04115 map04151 map04218 map04914 map04934 map05161 map05162 map05165 map05168 map05169 map05200 map05203 map05215 map05222 map05226

Pfam Domains

Protein Families

Pkinase

Protein Analysis

295

Amino Acids

33.75

Weight (kDa)

7.13

Isoelectric Point (pI)

30.65

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	4 - 287	2.4e-79	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	7 - 199	1.8e-32	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003086)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48750
fragaria_vesca	FvH4_1g27840 FvH4_1g27840 FvH4_1g27840
malus_domestica	MD02G1248900.v1.1 MD07G1067400.v1.1
prunus_persica	Prupe.2G084600_v2.0.a1 Prupe.2G084600_v2.0.a1 Prupe.2G084600_v2.0.a1
pyrus_communis	pycom02g21320
rosa_chinensis	RchiOBHm_Chr1g0333011 RchiOBHm_Chr1g0333051 RchiOBHm_Chr3g0489001
rosa_laevigata	RLG00000022900 RLG00000029629
rosa_multiflora	Rmu_co8022684.1_g000001 Rmu_sc0000117.1_g000041 Rmu_sc0000117.1_g000055 Rmu_sc0003088.1_g000001 Rmu_sc0021925.1_g000005
rosa_roxburghii	Rroxscaffold_4G00317830 Rroxscaffold_6G00393760
rosa_rugosa	Rorug01G0106600 Rorug03G0242500 Rorug03G0242500
rosa_samantha	Rh1AG129500 Rh1BG099000 Rh1CG123400 Rh1DG135300 Rh1DG135500 Rh3AG290900 Rh3BG327600 Rh3CG324900 Rh3DG323900
rosa_wichuraiana	Rw1G010280 Rw1G010330 Rw3G025740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AclWI	GGATC	3 cut(s)	264, 806, 819
AcoI	YGGCCR	1 cut(s)	831
AcsI	RAATTY	3 cut(s)	102, 287, 694
AcuI	CTGAAG	1 cut(s)	281
AcvI	CACGTG	1 cut(s)	65
AfaI	GTAC	7 cut(s)	11, 245, 415, 506, 664, 857, 879
AfeI	AGCGCT	1 cut(s)	224
AfiI	CCNNNNNNNGG	3 cut(s)	253, 731, 822
AflIII	ACRYGT	1 cut(s)	558
AgsI	TTSAA	4 cut(s)	97, 166, 643, 862
AhdI	GACNNNNNGTC	1 cut(s)	562
AjiI	CACGTC	1 cut(s)	559
AjnI	CCWGG	4 cut(s)	246, 613, 685, 833
AjuI	GAANNNNNNNTTGG	4 cut(s)	92, 124, 754, 786
AluBI	AGCT	3 cut(s)	92, 430, 776
AluI	AGCT	3 cut(s)	92, 430, 776
Alw21I	GWGCWC	2 cut(s)	213, 844
Alw26I	GTCTC	1 cut(s)	77
Alw44I	GTGCAC	1 cut(s)	209
AlwI	GGATC	3 cut(s)	264, 806, 819
AlwNI	CAGNNNCTG	1 cut(s)	397
Aor51HI	AGCGCT	1 cut(s)	224
AoxI	GGCC	3 cut(s)	602, 683, 831
ApaLI	GTGCAC	1 cut(s)	209
ApeKI	GCWGC	1 cut(s)	175
ApoI	RAATTY	3 cut(s)	102, 287, 694
ArsI	GACNNNNNNTTYG	2 cut(s)	76, 108
AspLEI	GCGC	2 cut(s)	225, 514
AspS9I	GGNCC	2 cut(s)	4, 298
AsuHPI	GGTGA	2 cut(s)	44, 505
AvaII	GGWCC	2 cut(s)	4, 298
BaeGI	GKGCMC	1 cut(s)	213
BamHI	GGATCC	1 cut(s)	811
BbrPI	CACGTG	1 cut(s)	65
Bbv12I	GWGCWC	2 cut(s)	213, 844
BbvI	GCAGC	1 cut(s)	187
BccI	CCATC	1 cut(s)	583
BceAI	ACGGC	2 cut(s)	617, 846
BciT130I	CCWGG	4 cut(s)	248, 615, 687, 835
BciVI	GTATCC	1 cut(s)	670
BcoDI	GTCTC	1 cut(s)	77
BfaI	CTAG	1 cut(s)	450
BfmI	CTRYAG	1 cut(s)	87
BfoI	RGCGCY	2 cut(s)	226, 515
BfuI	GTATCC	1 cut(s)	670
BglII	AGATCT	1 cut(s)	379
BisI	GCNGC	1 cut(s)	176
BlsI	GCNGC	1 cut(s)	177
BmcAI	AGTACT	1 cut(s)	857
Bme1390I	CCNGG	4 cut(s)	248, 615, 687, 835
Bme18I	GGWCC	2 cut(s)	4, 298
BmeRI	GACNNNNNGTC	1 cut(s)	562
BmgBI	CACGTC	1 cut(s)	559
BmgT120I	GGNCC	2 cut(s)	4, 298
BmiI	GGNNCC	2 cut(s)	300, 813
BmrFI	CCNGG	4 cut(s)	248, 615, 687, 835
BpmI	CTGGAG	2 cut(s)	534, 708
Bpu10I	CCTNAGC	1 cut(s)	738
BpuEI	CTTGAG	1 cut(s)	866
BsaAI	YACGTR	3 cut(s)	13, 65, 149
BsaJI	CCNNGG	2 cut(s)	614, 672
Bsc4I	CCNNNNNNNGG	3 cut(s)	253, 731, 822
Bse1I	ACTGG	3 cut(s)	7, 450, 551
Bse3DI	GCAATG	1 cut(s)	342
BseBI	CCWGG	4 cut(s)	248, 615, 687, 835
BseDI	CCNNGG	2 cut(s)	614, 672
BseGI	GGATG	1 cut(s)	208
BseLI	CCNNNNNNNGG	3 cut(s)	253, 731, 822
BseMI	GCAATG	1 cut(s)	342
BseMII	CTCAG	2 cut(s)	349, 615
BseNI	ACTGG	3 cut(s)	7, 450, 551
BseSI	GKGCMC	1 cut(s)	213
BseXI	GCAGC	1 cut(s)	187
BseYI	CCCAGC	1 cut(s)	815
Bsh1285I	CGRYCG	1 cut(s)	672
BshFI	GGCC	3 cut(s)	604, 685, 833
BsiEI	CGRYCG	1 cut(s)	672
BsiHKAI	GWGCWC	2 cut(s)	213, 844
BslFI	GGGAC	1 cut(s)	632
BslI	CCNNNNNNNGG	3 cut(s)	253, 731, 822
BsmAI	GTCTC	1 cut(s)	77
BsmFI	GGGAC	1 cut(s)	632
BsnI	GGCC	3 cut(s)	604, 685, 833
Bsp1286I	GDGCHC	2 cut(s)	213, 844
Bsp143I	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
BspANI	GGCC	3 cut(s)	604, 685, 833
BspCNI	CTCAG	2 cut(s)	348, 616
BspHI	TCATGA	1 cut(s)	484
BspLI	GGNNCC	2 cut(s)	300, 813
BspPI	GGATC	3 cut(s)	264, 806, 819
BsrDI	GCAATG	1 cut(s)	342
BsrI	ACTGG	3 cut(s)	7, 450, 551
BssECI	CCNNGG	2 cut(s)	614, 672
BssMI	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
Bst2UI	CCWGG	4 cut(s)	248, 615, 687, 835
Bst4CI	ACNGT	3 cut(s)	215, 318, 353
BstBAI	YACGTR	3 cut(s)	13, 65, 149
BstC8I	GCNNGC	1 cut(s)	432
BstDEI	CTNAG	3 cut(s)	335, 624, 738
BstEII	GGTNACC	1 cut(s)	493
BstF5I	GGATG	1 cut(s)	208
BstH2I	RGCGCY	2 cut(s)	226, 515
BstHHI	GCGC	2 cut(s)	225, 514
BstKTI	GATC	6 cut(s)	70, 259, 382, 409, 787, 814
BstMAI	GTCTC	1 cut(s)	77
BstMBI	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
BstMCI	CGRYCG	1 cut(s)	672
BstMWI	GCNNNNNNNGC	1 cut(s)	839
BstNI	CCWGG	4 cut(s)	248, 615, 687, 835
BstPI	GGTNACC	1 cut(s)	493
BstSCI	CCNGG	4 cut(s)	246, 613, 685, 833
BstSFI	CTRYAG	1 cut(s)	87
BstSLI	GKGCMC	1 cut(s)	213
BstV1I	GCAGC	1 cut(s)	187
BstX2I	RGATCY	3 cut(s)	256, 379, 811
BstYI	RGATCY	3 cut(s)	256, 379, 811
BsuI	GTATCC	1 cut(s)	670
BsuRI	GGCC	3 cut(s)	604, 685, 833
BtrI	CACGTC	1 cut(s)	559
BtsCI	GGATG	1 cut(s)	208
BtsI	GCAGTG	1 cut(s)	138
BtsIMutI	CAGTG	3 cut(s)	138, 220, 573
Cac8I	GCNNGC	1 cut(s)	432
CaiI	CAGNNNCTG	1 cut(s)	397
CciI	TCATGA	1 cut(s)	484
CfoI	GCGC	2 cut(s)	225, 514
Cfr13I	GGNCC	2 cut(s)	4, 298
Csp6I	GTAC	7 cut(s)	10, 244, 414, 505, 663, 856, 878
CspCI	CAANNNNNGTGG	2 cut(s)	535, 570
CviAII	CATG	5 cut(s)	179, 485, 658, 681, 851
CviJI	RGCY	7 cut(s)	92, 430, 449, 604, 685, 776, 833
CviKI_1	RGCY	7 cut(s)	92, 430, 449, 604, 685, 776, 833
CviQI	GTAC	7 cut(s)	10, 244, 414, 505, 663, 856, 878
DdeI	CTNAG	3 cut(s)	335, 624, 738
DpnI	GATC	6 cut(s)	69, 258, 381, 408, 786, 813
DpnII	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
DraI	TTTAAA	1 cut(s)	712
DriI	GACNNNNNGTC	1 cut(s)	562
EaeI	YGGCCR	1 cut(s)	831
Eam1105I	GACNNNNNGTC	1 cut(s)	562
Eco47I	GGWCC	2 cut(s)	4, 298
Eco47III	AGCGCT	1 cut(s)	224
Eco57I	CTGAAG	1 cut(s)	281
Eco72I	CACGTG	1 cut(s)	65
Eco91I	GGTNACC	1 cut(s)	493
EcoO109I	RGGNCCY	1 cut(s)	298
EcoO65I	GGTNACC	1 cut(s)	493
EcoRI	GAATTC	1 cut(s)	694
EcoRII	CCWGG	4 cut(s)	246, 613, 685, 833
FaeI	CATG	5 cut(s)	182, 488, 661, 684, 854
FaqI	GGGAC	1 cut(s)	632
FatI	CATG	5 cut(s)	178, 484, 657, 680, 850
FauNDI	CATATG	1 cut(s)	270
Fnu4HI	GCNGC	1 cut(s)	176
FokI	GGATG	1 cut(s)	215
Fsp4HI	GCNGC	1 cut(s)	176
FspBI	CTAG	1 cut(s)	450
GlaI	GCGC	2 cut(s)	224, 513
GluI	GCNGC	1 cut(s)	176
GsaI	CCCAGC	1 cut(s)	819
GsuI	CTGGAG	2 cut(s)	534, 708
HaeII	RGCGCY	2 cut(s)	226, 515
HaeIII	GGCC	3 cut(s)	604, 685, 833
HhaI	GCGC	2 cut(s)	225, 514
Hin1II	CATG	5 cut(s)	182, 488, 661, 684, 854
Hin6I	GCGC	2 cut(s)	223, 512
HinP1I	GCGC	2 cut(s)	223, 512
HincII	GTYRAC	1 cut(s)	556
HindII	GTYRAC	1 cut(s)	556
HindIII	AAGCTT	1 cut(s)	428
HinfI	GANTC	6 cut(s)	275, 331, 394, 620, 654, 770
HphI	GGTGA	2 cut(s)	44, 505
Hpy166II	GTNNAC	4 cut(s)	55, 211, 556, 595
Hpy188I	TCNGA	5 cut(s)	261, 338, 625, 669, 726
Hpy188III	TCNNGA	6 cut(s)	163, 284, 485, 643, 767, 845
Hpy8I	GTNNAC	4 cut(s)	55, 211, 556, 595
Hpy99I	CGWCG	1 cut(s)	414
HpyAV	CCTTC	1 cut(s)	29
HpyCH4III	ACNGT	3 cut(s)	215, 318, 353
HpyCH4IV	ACGT	4 cut(s)	12, 64, 148, 558
HpyCH4V	TGCA	5 cut(s)	175, 211, 293, 422, 434
HpyF10VI	GCNNNNNNNGC	1 cut(s)	839
HpyF3I	CTNAG	3 cut(s)	335, 624, 738
HpySE526I	ACGT	4 cut(s)	12, 64, 148, 558
Hsp92II	CATG	5 cut(s)	182, 488, 661, 684, 854
HspAI	GCGC	2 cut(s)	223, 512
Kzo9I	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
LmnI	GCTCC	1 cut(s)	112
Lsp1109I	GCAGC	1 cut(s)	187
MaeI	CTAG	1 cut(s)	450
MaeII	ACGT	4 cut(s)	12, 64, 148, 558
MaeIII	GTNAC	3 cut(s)	73, 195, 493
MalI	GATC	6 cut(s)	69, 258, 381, 408, 786, 813
MboI	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
MboII	GAAGA	7 cut(s)	37, 109, 131, 274, 362, 708, 779
MflI	RGATCY	3 cut(s)	256, 379, 811
MhlI	GDGCHC	2 cut(s)	213, 844
MluCI	AATT	4 cut(s)	102, 287, 635, 694
MlyI	GAGTC	1 cut(s)	614
MmeI	TCCRAC	1 cut(s)	692
MnlI	CCTC	7 cut(s)	118, 170, 312, 332, 481, 509, 667
MseI	TTAA	2 cut(s)	384, 711
MspA1I	CMGCKG	1 cut(s)	776
MspR9I	CCNGG	4 cut(s)	248, 615, 687, 835
MvaI	CCWGG	4 cut(s)	248, 615, 687, 835
MwoI	GCNNNNNNNGC	1 cut(s)	839
NdeI	CATATG	1 cut(s)	270
NdeII	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
NlaIII	CATG	5 cut(s)	182, 488, 661, 684, 854
NlaIV	GGNNCC	2 cut(s)	300, 813
NmeAIII	GCCGAG	1 cut(s)	610
NmuCI	GTSAC	2 cut(s)	73, 493
PagI	TCATGA	1 cut(s)	484
PfeI	GAWTC	5 cut(s)	275, 331, 394, 654, 770
PkrI	GCNGC	1 cut(s)	177
PleI	GAGTC	1 cut(s)	614
PmaCI	CACGTG	1 cut(s)	65
PmlI	CACGTG	1 cut(s)	65
PpsI	GAGTC	1 cut(s)	614
Ppu21I	YACGTR	3 cut(s)	13, 65, 149
PpuMI	RGGWCCY	1 cut(s)	298
Psp5II	RGGWCCY	1 cut(s)	298
Psp6I	CCWGG	4 cut(s)	246, 613, 685, 833
PspCI	CACGTG	1 cut(s)	65
PspEI	GGTNACC	1 cut(s)	493
PspFI	CCCAGC	1 cut(s)	815
PspGI	CCWGG	4 cut(s)	246, 613, 685, 833
PspN4I	GGNNCC	2 cut(s)	300, 813
PspPI	GGNCC	2 cut(s)	4, 298
PspPPI	RGGWCCY	1 cut(s)	298
PstNI	CAGNNNCTG	1 cut(s)	397
PsuI	RGATCY	3 cut(s)	256, 379, 811
PvuII	CAGCTG	1 cut(s)	776
RsaI	GTAC	7 cut(s)	11, 245, 415, 506, 664, 857, 879
RsaNI	GTAC	7 cut(s)	10, 244, 414, 505, 663, 856, 878
SaqAI	TTAA	2 cut(s)	384, 711
SatI	GCNGC	1 cut(s)	176
Sau3AI	GATC	6 cut(s)	67, 256, 379, 406, 784, 811
Sau96I	GGNCC	2 cut(s)	4, 298
ScaI	AGTACT	1 cut(s)	857
SchI	GAGTC	1 cut(s)	614
ScrFI	CCNGG	4 cut(s)	248, 615, 687, 835
SduI	GDGCHC	2 cut(s)	213, 844
SfcI	CTRYAG	1 cut(s)	87
SinI	GGWCC	2 cut(s)	4, 298
SmlI	CTYRAG	1 cut(s)	845
SmoI	CTYRAG	1 cut(s)	845
Sse9I	AATT	4 cut(s)	102, 287, 635, 694
SspMI	CTAG	1 cut(s)	450
StyD4I	CCNGG	4 cut(s)	246, 613, 685, 833
TaaI	ACNGT	3 cut(s)	215, 318, 353
TaiI	ACGT	4 cut(s)	15, 67, 151, 561
TaqII	GACCGA	1 cut(s)	686
TasI	AATT	4 cut(s)	102, 287, 635, 694
TatI	WGTACW	1 cut(s)	855
TfiI	GAWTC	5 cut(s)	275, 331, 394, 654, 770
Tru1I	TTAA	2 cut(s)	384, 711
Tru9I	TTAA	2 cut(s)	384, 711
TscAI	CASTG	3 cut(s)	145, 220, 573
TseFI	GTSAC	2 cut(s)	73, 493
TseI	GCWGC	1 cut(s)	175
Tsp45I	GTSAC	2 cut(s)	73, 493
TspDTI	ATGAA	2 cut(s)	362, 648
TspRI	CASTG	3 cut(s)	145, 220, 573
VneI	GTGCAC	1 cut(s)	209
VpaK11BI	GGWCC	2 cut(s)	4, 298
XapI	RAATTY	3 cut(s)	102, 287, 694
XcmI	CCANNNNNNNNNTGG	1 cut(s)	558
XspI	CTAG	1 cut(s)	450
ZrmI	AGTACT	1 cut(s)	857

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.