MD02G1253200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013724
Physical Location & Seq
Forward (+)
30484780 .. 30496056
11277 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1253200.v1.1.491

Sequence Viewer

Length: 834 bp
ATGTTTTTTCTCCTTTTTGGTTTTTTTCCCGGTTGTGTTTGTGCTGGGACAACATTTTCCACCTTCTGTTTGCACTGGGATGATTGCGTTTTAAAACTGTTGGGGAATTCTTTGATGGAAAACAGAGCTCTTACTCCTATCAATCAGTTGGTCCCGTACATAAATGCAGAAAAATCAAGGTTCGAGTTTGCAGAGTATGCAGACCAAGGTTTCCCGAAACTACTGGTGCATATTCAGGCTTGCATTGTATATTACTTACCTATTAAAAATTTGTTTGGAATTTGCTTTTCCCACAGAGGAGAAGAGCTGACAGTTACGCTGTGGGCAGATGTTGCTCGAAGTATTGCTTCTTTATCCATTGAATCATTGTCTCTGCCGATCATTGTGGTTTTCACAAGTTTAAAGGTCAAGCTGTACCTAGACAAGATAGTCTTGAACACCATTGGTTCATCACTTTTCTTCATTGATCTAGATATTCTGGAAGTAAATTCATACAAATCAGTCTTTTCCAAATGCACAGAACCTCTAAAAATACTGCCACCTTTTACAAAACAGGCCCGTGAAGCTCAAATCTTGCAGACAGCGAAGGTCACAATTGCAGACGCTGAAATTTTCATGCCAAAATATGATGTGGAGGATGAGAAATTGTTTGCTGTTGTTCTCTTTTCAGTTTTGCAGGCGATGATCTTTTACCAAATGTCGAGGAGCTTCCATGGGACATGCTCTATGCTCTCGCTTCCCAGTTGCCTCTGTTGGGGTTGGAAAAGCTACAGATCACAATGTAAGTCAAAAACAGTAAAGTTCCCAAGATGTTCTGTGCTGGAGCAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
DUF223 Rep_fac-A_C

Protein Analysis

278

Amino Acids

31.46

Weight (kDa)

7.92

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 428
AcsI RAATTY 5 cut(s) 106, 268, 279, 487, 609
AfaI GTAC 2 cut(s) 158, 416
AfiI CCNNNNNNNGG 1 cut(s) 754
AgsI TTSAA 2 cut(s) 362, 436
AluBI AGCT 6 cut(s) 128, 307, 412, 566, 708, 768
AluI AGCT 6 cut(s) 128, 307, 412, 566, 708, 768
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 1 cut(s) 375
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 1 cut(s) 555
ApoI RAATTY 5 cut(s) 106, 268, 279, 487, 609
AspS9I GGNCC 2 cut(s) 151, 556
AsuC2I CCSGG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 130
Bbv12I GWGCWC 1 cut(s) 130
BccI CCATC 1 cut(s) 109
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 2 cut(s) 419, 470
BfmI CTRYAG 1 cut(s) 769
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 151, 556
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 2 cut(s) 85, 735
BmuI ACTGGG 2 cut(s) 85, 735
BpuMI CCSGG 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 205, 712
Bsc4I CCNNNNNNNGG 1 cut(s) 754
Bse1I ACTGG 3 cut(s) 80, 228, 741
BseDI CCNNGG 2 cut(s) 205, 712
BseGI GGATG 2 cut(s) 85, 643
BseLI CCNNNNNNNGG 1 cut(s) 754
BseNI ACTGG 3 cut(s) 80, 228, 741
BseRI GAGGAG 2 cut(s) 312, 718
BseYI CCCAGC 1 cut(s) 44
BshFI GGCC 1 cut(s) 557
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 3 cut(s) 61, 137, 730
BslI CCNNNNNNNGG 1 cut(s) 754
BsmAI GTCTC 1 cut(s) 375
BsmFI GGGAC 3 cut(s) 61, 137, 730
BsnI GGCC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 4 cut(s) 378, 466, 684, 773
Bsp19I CCATGG 1 cut(s) 712
BspANI GGCC 1 cut(s) 557
BspLI GGNNCC 1 cut(s) 153
BspQI GCTCTTC 1 cut(s) 297
BsrI ACTGG 3 cut(s) 80, 228, 741
BssECI CCNNGG 2 cut(s) 205, 712
BssMI GATC 4 cut(s) 378, 466, 684, 773
BssT1I CCWWGG 2 cut(s) 205, 712
Bst4CI ACNGT 3 cut(s) 99, 313, 796
Bst6I CTCTTC 1 cut(s) 297
BstAPI GCANNNNNTGC 2 cut(s) 197, 332
BstC8I GCNNGC 2 cut(s) 241, 678
BstDSI CCRYGG 1 cut(s) 712
BstF5I GGATG 2 cut(s) 85, 643
BstKTI GATC 4 cut(s) 381, 469, 687, 776
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 4 cut(s) 378, 466, 684, 773
BstMWI GCNNNNNNNGC 3 cut(s) 197, 332, 563
BstNSI RCATGY 1 cut(s) 723
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 1 cut(s) 769
BsuRI GGCC 1 cut(s) 557
BtgI CCRYGG 1 cut(s) 712
BtgZI GCGATG 1 cut(s) 695
BtsCI GGATG 2 cut(s) 85, 643
BtsIMutI CAGTG 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 241, 678
CaiI CAGNNNCTG 1 cut(s) 605
Cfr13I GGNCC 2 cut(s) 151, 556
CseI GACGC 1 cut(s) 611
Csp6I GTAC 2 cut(s) 157, 415
CviAII CATG 3 cut(s) 616, 713, 720
CviJI RGCY 8 cut(s) 128, 239, 307, 412, 557, 566, 708, 768
CviKI_1 RGCY 8 cut(s) 128, 239, 307, 412, 557, 566, 708, 768
CviQI GTAC 2 cut(s) 157, 415
DpnI GATC 4 cut(s) 380, 468, 686, 775
DpnII GATC 4 cut(s) 378, 466, 684, 773
DraI TTTAAA 2 cut(s) 93, 402
DrdI GACNNNNNNGTC 1 cut(s) 428
DseDI GACNNNNNNGTC 1 cut(s) 428
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Ecl136II GAGCTC 1 cut(s) 128
Eco130I CCWWGG 2 cut(s) 205, 712
Eco24I GRGCYC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 151
Eco53kI GAGCTC 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 128
EcoRI GAATTC 1 cut(s) 106
EcoT14I CCWWGG 2 cut(s) 205, 712
EcoT38I GRGCYC 1 cut(s) 130
ErhI CCWWGG 2 cut(s) 205, 712
FaeI CATG 3 cut(s) 619, 716, 723
FalI AAGNNNNNCTT 4 cut(s) 331, 363, 416, 448
FaqI GGGAC 3 cut(s) 61, 137, 730
FatI CATG 3 cut(s) 615, 712, 719
FokI GGATG 2 cut(s) 92, 650
FriOI GRGCYC 1 cut(s) 130
FspBI CTAG 2 cut(s) 419, 470
GsaI CCCAGC 1 cut(s) 48
HaeIII GGCC 1 cut(s) 557
HapII CCGG 1 cut(s) 30
HgaI GACGC 1 cut(s) 611
Hin1II CATG 3 cut(s) 619, 716, 723
HinfI GANTC 1 cut(s) 362
HpaII CCGG 1 cut(s) 30
Hpy188III TCNNGA 4 cut(s) 214, 433, 470, 479
HpyAV CCTTC 2 cut(s) 73, 580
HpyCH4III ACNGT 3 cut(s) 99, 313, 796
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 332, 563
Hsp92II CATG 3 cut(s) 619, 716, 723
Kzo9I GATC 4 cut(s) 378, 466, 684, 773
LguI GCTCTTC 1 cut(s) 297
LmnI GCTCC 2 cut(s) 705, 823
MaeI CTAG 2 cut(s) 419, 470
MaeIII GTNAC 2 cut(s) 313, 589
MalI GATC 4 cut(s) 380, 468, 686, 775
MboI GATC 4 cut(s) 378, 466, 684, 773
MboII GAAGA 2 cut(s) 314, 451
MfeI CAATTG 1 cut(s) 594
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 7 cut(s) 106, 268, 279, 487, 594, 609, 644
MmeI TCCRAC 1 cut(s) 740
MnlI CCTC 5 cut(s) 290, 534, 628, 696, 758
MseI TTAA 4 cut(s) 92, 264, 401, 832
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 1 cut(s) 30
MunI CAATTG 1 cut(s) 594
MwoI GCNNNNNNNGC 3 cut(s) 197, 332, 563
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 712
NdeII GATC 4 cut(s) 378, 466, 684, 773
NlaIII CATG 3 cut(s) 619, 716, 723
NlaIV GGNNCC 1 cut(s) 153
NmuCI GTSAC 1 cut(s) 589
NspI RCATGY 1 cut(s) 723
PciSI GCTCTTC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 362
Psp124BI GAGCTC 1 cut(s) 130
PspFI CCCAGC 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 151, 556
PstNI CAGNNNCTG 1 cut(s) 605
RsaI GTAC 2 cut(s) 158, 416
RsaNI GTAC 2 cut(s) 157, 415
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 130
SapI GCTCTTC 1 cut(s) 297
SaqAI TTAA 4 cut(s) 92, 264, 401, 832
Sau3AI GATC 4 cut(s) 378, 466, 684, 773
Sau96I GGNCC 2 cut(s) 151, 556
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 1 cut(s) 130
SfcI CTRYAG 1 cut(s) 769
SinI GGWCC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 78
Sse9I AATT 7 cut(s) 106, 268, 279, 487, 594, 609, 644
SspMI CTAG 2 cut(s) 419, 470
SstI GAGCTC 1 cut(s) 130
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 2 cut(s) 205, 712
TaaI ACNGT 3 cut(s) 99, 313, 796
TaqI TCGA 3 cut(s) 183, 337, 701
TasI AATT 7 cut(s) 106, 268, 279, 487, 594, 609, 644
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 4 cut(s) 92, 264, 401, 832
Tru9I TTAA 4 cut(s) 92, 264, 401, 832
TscAI CASTG 1 cut(s) 80
TseFI GTSAC 1 cut(s) 589
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 4 cut(s) 438, 451, 480, 604
TspRI CASTG 1 cut(s) 80
VpaK11BI GGWCC 1 cut(s) 151
XapI RAATTY 5 cut(s) 106, 268, 279, 487, 609
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 1 cut(s) 723
XspI CTAG 2 cut(s) 419, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.