MD10G1011600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013724
Physical Location & Seq
Reverse (-)
1576784 .. 1580676
3893 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1011600.v1.1.491

Sequence Viewer

Length: 855 bp
ATGCGCTGGGTTTTTTTTTTTTCCCTTCTTTCTAGGTTTGCAGGTTGGGCTCAGGTTGGGGGAGCGGAAGATGTAAAGATGGGTGTACGGAGGAGGTGGAGGATGGGTGCGCACAACGGATGGAAGATGATTTGGACGGGGGTTGCTGAGATATGGACTTCGCCGCCGCCCCGGGGAGGGGGTGTGGTTTTGGGGGGCATGTGGGATATCATATATACTAAAACTCAAACCCCCTGGTCACTGTGGATGACCAGTGTGTGGACGTTTTTGCCCCTCCGTTTTTCCTCTGTTTCTCTAGCTGTTTCCATCACGTTTCTGTACAGCGAAGTGTCGGATTTACCCTTTCTGGCCACGCGTCTCTCATCGCTTCACCTTCTGTTTCTCGACTCTTCTACTTCATTCCACGTTTTTCCATTTGTGTTCACTTCTCTCTGTCTCTCTCTCAAATCTCTGCTTCCGTCGCACTCGAACTCCTTCTGTCGCTCTTCTCTTTCTAAAATCCATCTAAAAAAATGTATAATGGGTATTGTTTCTCTCTGCAGGTTACTCGAAGAAGTCTGTGAGGGGTCAATAGGAGAAGGTGGATTGCGTTTCAAAGTGCAAAAAATACAGGGCATGCCTCTTTATGCGTTTCCAATCTTCCAAAAATCGTGTTCGTGGAGGCGATTTGAGACGGATTTGAAGCTCGGCTACAGAGATTTTATTCAATTTGGTGGATTTGGAAATTTGTACCAATTTGGTTTCGATTGGAATTTCTGGGCTCATCTAATCCAACGTTTCCAGGTGAGAAAGTTGCTTAAATTTCGATTTTTATTAAACTCAGGTGAGCTTCTTCAGTTTTTTAGATTGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

32.84

Weight (kDa)

10.03

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 111
Acc36I ACCTGC 2 cut(s) 32, 531
AccB7I CCANNNNNTGG 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 65
AccII CGCG 1 cut(s) 355
AciI CCGC 3 cut(s) 65, 164, 167
AclI AACGTT 1 cut(s) 775
AcoI YGGCCR 1 cut(s) 348
AcsI RAATTY 3 cut(s) 724, 751, 800
AcuI CTGAAG 1 cut(s) 818
AfaI GTAC 3 cut(s) 87, 320, 731
AfiI CCNNNNNNNGG 5 cut(s) 173, 176, 177, 178, 258
AflIII ACRYGT 1 cut(s) 353
AgsI TTSAA 3 cut(s) 595, 682, 707
AjnI CCWGG 2 cut(s) 233, 780
AluBI AGCT 3 cut(s) 299, 685, 829
AluI AGCT 3 cut(s) 299, 685, 829
Alw26I GTCTC 3 cut(s) 362, 440, 665
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 1 cut(s) 348
ApoI RAATTY 3 cut(s) 724, 751, 800
Asp700I GAANNNNTTC 1 cut(s) 473
AspLEI GCGC 2 cut(s) 6, 112
AsuC2I CCSGG 2 cut(s) 172, 173
AsuHPI GGTGA 3 cut(s) 362, 796, 836
AvaI CYCGRG 1 cut(s) 171
BalI TGGCCA 1 cut(s) 350
BanII GRGCYC 2 cut(s) 52, 763
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BccI CCATC 5 cut(s) 73, 97, 114, 314, 510
BcgI CGANNNNNNTGC 2 cut(s) 529, 563
BciT130I CCWGG 2 cut(s) 235, 782
BcnI CCSGG 2 cut(s) 172, 173
BcoDI GTCTC 3 cut(s) 362, 440, 665
BfaI CTAG 2 cut(s) 33, 296
BfmI CTRYAG 2 cut(s) 538, 691
BfuAI ACCTGC 2 cut(s) 32, 531
BisI GCNGC 2 cut(s) 164, 167
BlsI GCNGC 2 cut(s) 165, 168
Bme1390I CCNGG 4 cut(s) 172, 173, 235, 782
BmeT110I CYCGRG 1 cut(s) 171
BmrFI CCNGG 4 cut(s) 172, 173, 235, 782
Bpu10I CCTNAGC 1 cut(s) 51
BpuMI CCSGG 2 cut(s) 172, 173
BsaJI CCNNGG 4 cut(s) 170, 171, 172, 233
BsaXI ACNNNNNCTCC 6 cut(s) 91, 121, 168, 198, 455, 485
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 176, 177, 178, 258
Bse1I ACTGG 1 cut(s) 252
BseBI CCWGG 2 cut(s) 235, 782
BseDI CCNNGG 4 cut(s) 170, 171, 172, 233
BseGI GGATG 3 cut(s) 108, 125, 252
BseLI CCNNNNNNNGG 5 cut(s) 173, 176, 177, 178, 258
BseMII CTCAG 3 cut(s) 65, 138, 834
BseNI ACTGG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 106
BseYI CCCAGC 1 cut(s) 6
Bsh1236I CGCG 1 cut(s) 355
BshFI GGCC 1 cut(s) 350
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 1 cut(s) 172
BslI CCNNNNNNNGG 5 cut(s) 173, 176, 177, 178, 258
BsmAI GTCTC 3 cut(s) 362, 440, 665
BsmBI CGTCTC 2 cut(s) 362, 665
BsnI GGCC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 2 cut(s) 52, 763
Bsp1407I TGTACA 1 cut(s) 318
BspACI CCGC 3 cut(s) 65, 164, 167
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 3 cut(s) 64, 139, 833
BspFNI CGCG 1 cut(s) 355
BspMAI CTGCAG 1 cut(s) 542
BspMI ACCTGC 2 cut(s) 32, 531
BspQI GCTCTTC 1 cut(s) 490
BsrBI CCGCTC 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 4 cut(s) 170, 171, 172, 233
Bst2UI CCWGG 2 cut(s) 235, 782
Bst4CI ACNGT 1 cut(s) 243
Bst6I CTCTTC 2 cut(s) 394, 490
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 1 cut(s) 617
BstDEI CTNAG 3 cut(s) 51, 147, 820
BstF5I GGATG 3 cut(s) 108, 125, 252
BstFNI CGCG 1 cut(s) 355
BstHHI GCGC 2 cut(s) 6, 112
BstMAI GTCTC 3 cut(s) 362, 440, 665
BstMWI GCNNNNNNNGC 2 cut(s) 47, 460
BstNI CCWGG 2 cut(s) 235, 782
BstNSI RCATGY 2 cut(s) 202, 619
BstSCI CCNGG 4 cut(s) 170, 171, 233, 780
BstSFI CTRYAG 2 cut(s) 538, 691
BstUI CGCG 1 cut(s) 355
BsuRI GGCC 1 cut(s) 350
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 3 cut(s) 108, 125, 252
BtsIMutI CAGTG 2 cut(s) 239, 259
BveI ACCTGC 2 cut(s) 32, 531
Cac8I GCNNGC 1 cut(s) 617
CfoI GCGC 2 cut(s) 6, 112
Cfr9I CCCGGG 1 cut(s) 171
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 86, 319, 730
CviAII CATG 2 cut(s) 199, 616
CviJI RGCY 7 cut(s) 50, 299, 350, 685, 690, 761, 829
CviKI_1 RGCY 7 cut(s) 50, 299, 350, 685, 690, 761, 829
CviQI GTAC 3 cut(s) 86, 319, 730
DdeI CTNAG 3 cut(s) 51, 147, 820
EaeI YGGCCR 1 cut(s) 348
Eam1104I CTCTTC 2 cut(s) 394, 490
EarI CTCTTC 2 cut(s) 394, 490
Eco24I GRGCYC 2 cut(s) 52, 763
Eco32I GATATC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 233, 780
EcoRV GATATC 1 cut(s) 208
EcoT38I GRGCYC 2 cut(s) 52, 763
Esp3I CGTCTC 2 cut(s) 362, 665
FaeI CATG 2 cut(s) 202, 619
FaiI YATR 8 cut(s) 154, 200, 212, 214, 216, 518, 617, 627
FatI CATG 2 cut(s) 198, 615
Fnu4HI GCNGC 2 cut(s) 164, 167
FokI GGATG 3 cut(s) 115, 132, 259
FriOI GRGCYC 2 cut(s) 52, 763
Fsp4HI GCNGC 2 cut(s) 164, 167
FspAI RTGCGCAY 1 cut(s) 111
FspBI CTAG 2 cut(s) 33, 296
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 2 cut(s) 5, 111
GluI GCNGC 2 cut(s) 164, 167
GsaI CCCAGC 1 cut(s) 10
HaeIII GGCC 1 cut(s) 350
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 344
HhaI GCGC 2 cut(s) 6, 112
Hin1II CATG 2 cut(s) 202, 619
Hin6I GCGC 2 cut(s) 4, 110
HinP1I GCGC 2 cut(s) 4, 110
HinfI GANTC 1 cut(s) 386
HpaII CCGG 1 cut(s) 172
HphI GGTGA 3 cut(s) 362, 796, 836
Hpy166II GTNNAC 3 cut(s) 86, 261, 423
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 1 cut(s) 383
Hpy8I GTNNAC 3 cut(s) 86, 261, 423
Hpy99I CGWCG 1 cut(s) 463
HpyAV CCTTC 4 cut(s) 35, 383, 484, 572
HpyCH4III ACNGT 1 cut(s) 243
HpyCH4IV ACGT 4 cut(s) 263, 311, 405, 775
HpyCH4V TGCA 3 cut(s) 41, 540, 601
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 460
HpyF3I CTNAG 3 cut(s) 51, 147, 820
HpySE526I ACGT 4 cut(s) 263, 311, 405, 775
Hsp92II CATG 2 cut(s) 202, 619
HspAI GCGC 2 cut(s) 4, 110
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 1 cut(s) 62
MaeI CTAG 2 cut(s) 33, 296
MaeII ACGT 4 cut(s) 263, 311, 405, 775
MaeIII GTNAC 2 cut(s) 237, 543
MbiI CCGCTC 1 cut(s) 65
MboII GAAGA 7 cut(s) 80, 136, 381, 477, 563, 631, 824
MhlI GDGCHC 2 cut(s) 52, 763
MlsI TGGCCA 1 cut(s) 350
MluCI AATT 5 cut(s) 707, 724, 734, 751, 800
MluI ACGCGT 1 cut(s) 353
MluNI TGGCCA 1 cut(s) 350
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 312, 796
MnlI CCTC 9 cut(s) 84, 87, 93, 170, 284, 295, 556, 630, 654
Mox20I TGGCCA 1 cut(s) 350
MroXI GAANNNNTTC 1 cut(s) 473
MscI TGGCCA 1 cut(s) 350
MseI TTAA 2 cut(s) 798, 815
Msp20I TGGCCA 1 cut(s) 350
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 4 cut(s) 172, 173, 235, 782
MvaI CCWGG 2 cut(s) 235, 782
MvnI CGCG 1 cut(s) 355
MwoI GCNNNNNNNGC 2 cut(s) 47, 460
NciI CCSGG 2 cut(s) 172, 173
NlaIII CATG 2 cut(s) 202, 619
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 1 cut(s) 237
NsbI TGCGCA 1 cut(s) 111
NspI RCATGY 2 cut(s) 202, 619
PaeI GCATGC 1 cut(s) 619
PciSI GCTCTTC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 473
PflMI CCANNNNNTGG 1 cut(s) 258
PkrI GCNGC 2 cut(s) 165, 168
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
Psp1406I AACGTT 1 cut(s) 775
Psp6I CCWGG 2 cut(s) 233, 780
PspFI CCCAGC 1 cut(s) 6
PspGI CCWGG 2 cut(s) 233, 780
PstI CTGCAG 1 cut(s) 542
RsaI GTAC 3 cut(s) 87, 320, 731
RsaNI GTAC 3 cut(s) 86, 319, 730
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 2 cut(s) 798, 815
SatI GCNGC 2 cut(s) 164, 167
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 4 cut(s) 172, 173, 235, 782
SduI GDGCHC 2 cut(s) 52, 763
SfcI CTRYAG 2 cut(s) 538, 691
SmaI CCCGGG 1 cut(s) 173
SphI GCATGC 1 cut(s) 619
Sse9I AATT 5 cut(s) 707, 724, 734, 751, 800
SsiI CCGC 3 cut(s) 65, 164, 167
SspMI CTAG 2 cut(s) 33, 296
StyD4I CCNGG 4 cut(s) 170, 171, 233, 780
TaaI ACNGT 1 cut(s) 243
TaiI ACGT 4 cut(s) 266, 314, 408, 778
TaqI TCGA 5 cut(s) 384, 467, 549, 744, 805
TasI AATT 5 cut(s) 707, 724, 734, 751, 800
TatI WGTACW 1 cut(s) 318
TauI GCSGC 2 cut(s) 166, 169
Tru1I TTAA 2 cut(s) 798, 815
Tru9I TTAA 2 cut(s) 798, 815
TscAI CASTG 2 cut(s) 246, 259
TseFI GTSAC 1 cut(s) 237
Tsp45I GTSAC 1 cut(s) 237
TspDTI ATGAA 1 cut(s) 387
TspGWI ACGGA 5 cut(s) 103, 132, 266, 447, 689
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 2 cut(s) 246, 259
Van91I CCANNNNNTGG 1 cut(s) 258
XapI RAATTY 3 cut(s) 724, 751, 800
XceI RCATGY 2 cut(s) 202, 619
XmaI CCCGGG 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 473
XspI CTAG 2 cut(s) 33, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.