MD02G1254000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013724
Physical Location & Seq
Forward (+)
30559213 .. 30563052
3840 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1254000.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGTCCCGTACATAAATGCAGAAAAATCAAGGTTCGAGTTTGCAGAGTATGCAGACCAAGGTTTCCCGAAACTACTGGTGCATATTCAGGCTTGCATTGTATATTACTTACCTATTAAAAATTTGTTTGGAATTTGCTTTTCCCACAGAGGAGAAGAGCTGACAGTTACGCTGTGGGCAGATGTTGCTCGAAGTATTGCTTCTTTATCCATTGAATCATTGTCTCTGCCGATCATTGTGGTTTTCACAAGTTTAAAGGTCAAGCTGTACCTAGACAAGATAGTCTTGAACACCATTGGTTCATCACTTTTCTTCATTGATCTAGATATTCTGGAAGTAAATTCATACAAATCAGTCTTTTCCAAATGCACAGAACCTCTAAAAATACTGCCACCTTTTACAAAACAGGCCCGTGAAGCTCAAATCTTGCAGACAGCGAAGGTCACAATTGCAGACGCTGAAATTTTCATGCCAAAATATGATGTGGAGGATGAGAAATTGTTTGCTGTTGTTCTCTTTTCAGTTTTGCAGGCGATGATCTTTTACCAAATGTCGAGGAGCTTCCATGGGACATGCTCTATGCTCTCGCTTCCCAGTTGCCTCTGTTGGGGTTGGAAAAGCTACAGATCACAATGTAAGTCAAAAACAGTAAAGTTCCCAAGATGTTCTGTTAGGTTGGAGAGGTTGGAGGTGAGAAATGCAATTGGGAAAGATACGCTGAGAGTTTTACAGAACACCTTTGAAGCTGTCAGTGAGGATTCTTCTGCTAATGTTTTGTTGATCAGCAGTAAAGTTCCCAAGATGTACTGTGCTGTAGCAGATTTAAAGTTGGTCAGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
DUF223 Rep_fac-A_C

Protein Analysis

281

Amino Acids

31.77

Weight (kDa)

8.77

Isoelectric Point (pI)

47.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 281
AcsI RAATTY 4 cut(s) 121, 132, 340, 462
AfaI GTAC 3 cut(s) 11, 269, 806
AfiI CCNNNNNNNGG 1 cut(s) 607
AgsI TTSAA 3 cut(s) 215, 289, 743
AluBI AGCT 6 cut(s) 160, 265, 419, 561, 621, 746
AluI AGCT 6 cut(s) 160, 265, 419, 561, 621, 746
Alw26I GTCTC 1 cut(s) 228
AlwNI CAGNNNCTG 2 cut(s) 458, 840
AoxI GGCC 1 cut(s) 408
ApoI RAATTY 4 cut(s) 121, 132, 340, 462
AspS9I GGNCC 2 cut(s) 4, 409
AsuHPI GGTGA 1 cut(s) 703
AvaII GGWCC 1 cut(s) 4
BclI TGATCA 1 cut(s) 780
BcoDI GTCTC 1 cut(s) 228
BfaI CTAG 2 cut(s) 272, 323
BfmI CTRYAG 2 cut(s) 622, 813
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 2 cut(s) 4, 409
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 588
BmuI ACTGGG 1 cut(s) 588
BsaJI CCNNGG 2 cut(s) 58, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse1I ACTGG 2 cut(s) 81, 594
BseDI CCNNGG 2 cut(s) 58, 565
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMII CTCAG 1 cut(s) 710
BseNI ACTGG 2 cut(s) 81, 594
BseRI GAGGAG 2 cut(s) 165, 571
BshFI GGCC 1 cut(s) 410
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 1 cut(s) 607
BsmAI GTCTC 1 cut(s) 228
BsmFI GGGAC 1 cut(s) 583
BsnI GGCC 1 cut(s) 410
Bsp143I GATC 5 cut(s) 231, 319, 537, 626, 780
Bsp19I CCATGG 1 cut(s) 565
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 1 cut(s) 711
BspLI GGNNCC 1 cut(s) 6
BspQI GCTCTTC 1 cut(s) 150
BsrI ACTGG 2 cut(s) 81, 594
BssECI CCNNGG 2 cut(s) 58, 565
BssMI GATC 5 cut(s) 231, 319, 537, 626, 780
BssT1I CCWWGG 2 cut(s) 58, 565
Bst4CI ACNGT 3 cut(s) 166, 649, 809
Bst6I CTCTTC 1 cut(s) 150
BstAPI GCANNNNNTGC 2 cut(s) 50, 185
BstC8I GCNNGC 2 cut(s) 94, 531
BstDEI CTNAG 1 cut(s) 719
BstDSI CCRYGG 1 cut(s) 565
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 5 cut(s) 234, 322, 540, 629, 783
BstMAI GTCTC 1 cut(s) 228
BstMBI GATC 5 cut(s) 231, 319, 537, 626, 780
BstMWI GCNNNNNNNGC 3 cut(s) 50, 185, 416
BstNSI RCATGY 1 cut(s) 576
BstSFI CTRYAG 2 cut(s) 622, 813
BsuRI GGCC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 565
BtgZI GCGATG 1 cut(s) 548
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 2 cut(s) 757, 838
Cac8I GCNNGC 2 cut(s) 94, 531
CaiI CAGNNNCTG 2 cut(s) 458, 840
Cfr13I GGNCC 2 cut(s) 4, 409
CseI GACGC 1 cut(s) 464
Csp6I GTAC 3 cut(s) 10, 268, 805
CviAII CATG 3 cut(s) 469, 566, 573
CviJI RGCY 8 cut(s) 92, 160, 265, 410, 419, 561, 621, 746
CviKI_1 RGCY 8 cut(s) 92, 160, 265, 410, 419, 561, 621, 746
CviQI GTAC 3 cut(s) 10, 268, 805
DdeI CTNAG 1 cut(s) 719
DpnI GATC 5 cut(s) 233, 321, 539, 628, 782
DpnII GATC 5 cut(s) 231, 319, 537, 626, 780
DraI TTTAAA 2 cut(s) 255, 825
DrdI GACNNNNNNGTC 1 cut(s) 281
DseDI GACNNNNNNGTC 1 cut(s) 281
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 58, 565
Eco47I GGWCC 1 cut(s) 4
EcoT14I CCWWGG 2 cut(s) 58, 565
ErhI CCWWGG 2 cut(s) 58, 565
FaeI CATG 3 cut(s) 472, 569, 576
FalI AAGNNNNNCTT 4 cut(s) 184, 216, 269, 301
FaqI GGGAC 1 cut(s) 583
FatI CATG 3 cut(s) 468, 565, 572
FbaI TGATCA 1 cut(s) 780
FokI GGATG 1 cut(s) 503
FspBI CTAG 2 cut(s) 272, 323
HaeIII GGCC 1 cut(s) 410
HgaI GACGC 1 cut(s) 464
Hin1II CATG 3 cut(s) 472, 569, 576
HinfI GANTC 2 cut(s) 215, 758
HphI GGTGA 1 cut(s) 703
Hpy188III TCNNGA 4 cut(s) 67, 286, 323, 332
HpyAV CCTTC 1 cut(s) 433
HpyCH4III ACNGT 3 cut(s) 166, 649, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 185, 416
HpyF3I CTNAG 1 cut(s) 719
Hsp92II CATG 3 cut(s) 472, 569, 576
Ksp22I TGATCA 1 cut(s) 780
Kzo9I GATC 5 cut(s) 231, 319, 537, 626, 780
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 558
LpnPI CCDG 7 cut(s) 62, 74, 317, 392, 515, 607, 820
MaeI CTAG 2 cut(s) 272, 323
MaeIII GTNAC 2 cut(s) 166, 442
MalI GATC 5 cut(s) 233, 321, 539, 628, 782
MboI GATC 5 cut(s) 231, 319, 537, 626, 780
MboII GAAGA 3 cut(s) 167, 304, 753
MfeI CAATTG 2 cut(s) 447, 702
MluCI AATT 7 cut(s) 121, 132, 340, 447, 462, 497, 702
MmeI TCCRAC 3 cut(s) 593, 657, 666
MnlI CCTC 8 cut(s) 143, 387, 481, 549, 611, 675, 682, 748
MseI TTAA 3 cut(s) 117, 254, 824
MunI CAATTG 2 cut(s) 447, 702
MwoI GCNNNNNNNGC 3 cut(s) 50, 185, 416
NcoI CCATGG 1 cut(s) 565
NdeII GATC 5 cut(s) 231, 319, 537, 626, 780
NlaIII CATG 3 cut(s) 472, 569, 576
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 442
NspI RCATGY 1 cut(s) 576
PciSI GCTCTTC 1 cut(s) 150
PfeI GAWTC 2 cut(s) 215, 758
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 4, 409
PstNI CAGNNNCTG 2 cut(s) 458, 840
RsaI GTAC 3 cut(s) 11, 269, 806
RsaNI GTAC 3 cut(s) 10, 268, 805
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 3 cut(s) 117, 254, 824
Sau3AI GATC 5 cut(s) 231, 319, 537, 626, 780
Sau96I GGNCC 2 cut(s) 4, 409
SfcI CTRYAG 2 cut(s) 622, 813
SinI GGWCC 1 cut(s) 4
Sse9I AATT 7 cut(s) 121, 132, 340, 447, 462, 497, 702
SspMI CTAG 2 cut(s) 272, 323
StyI CCWWGG 2 cut(s) 58, 565
TaaI ACNGT 3 cut(s) 166, 649, 809
TaqI TCGA 3 cut(s) 36, 190, 554
TasI AATT 7 cut(s) 121, 132, 340, 447, 462, 497, 702
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 2 cut(s) 215, 758
Tru1I TTAA 3 cut(s) 117, 254, 824
Tru9I TTAA 3 cut(s) 117, 254, 824
TscAI CASTG 1 cut(s) 757
TseFI GTSAC 1 cut(s) 442
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 4 cut(s) 291, 304, 333, 457
TspRI CASTG 1 cut(s) 757
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 4 cut(s) 121, 132, 340, 462
XbaI TCTAGA 1 cut(s) 322
XceI RCATGY 1 cut(s) 576
XspI CTAG 2 cut(s) 272, 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.