MD03G1270800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013724
Physical Location & Seq
Forward (+)
35444601 .. 35447085
2485 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1270800.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGGTATTGTTTCTCTCTGCAGGTTACTTGGAGAAGTCTGTGAAGGGTCAATAGGAAAAGGTGGATTGCGTTTCAAAATACAAAAAATACAGGGCCTGCCTCTCTATGCGTTTCCAGTCTTCCAAAAATCTTGTTCGTGGAGGCGATTTGAGACGGATTTGAAGCTCGGCTTCAGAGATTTGATTCAAATTGGTGGATTTGGGTGCTTCCAAGGGACCTGCTCTATGCTCTTGCTTCGTGGTTGCCTCTGTTGGGTTTGGAAAAGCTACAGACAGCATTCATTTGGTGATGACTGGTTTGTTACTTTGATCACTGGTAGATTTGAGCGCCCTCCCCTCACTCTCTCGATCATATATTGGTCCTTCTTTCAAGGTAGTCAATGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

130

Amino Acids

14.79

Weight (kDa)

9.18

Isoelectric Point (pI)

25.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 12, 227
AcuI CTGAAG 1 cut(s) 157
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 4 cut(s) 76, 163, 188, 371
AluBI AGCT 2 cut(s) 166, 267
AluI AGCT 2 cut(s) 166, 267
Alw26I GTCTC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 1 cut(s) 94
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 3 cut(s) 94, 216, 360
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 2 cut(s) 216, 360
BbsI GAAGAC 1 cut(s) 112
BclI TGATCA 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 146
BfmI CTRYAG 2 cut(s) 19, 268
BfoI RGCGCY 1 cut(s) 331
BfuAI ACCTGC 2 cut(s) 12, 227
Bme18I GGWCC 2 cut(s) 216, 360
BmgT120I GGNCC 3 cut(s) 94, 216, 360
BmiI GGNNCC 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 253
Bse1I ACTGG 3 cut(s) 116, 299, 319
BseDI CCNNGG 1 cut(s) 211
BseLI CCNNNNNNNGG 1 cut(s) 253
BseNI ACTGG 3 cut(s) 116, 299, 319
BshFI GGCC 1 cut(s) 96
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 253
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 1 cut(s) 229
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 309, 348
BspANI GGCC 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 217
BspMAI CTGCAG 1 cut(s) 23
BspMI ACCTGC 2 cut(s) 12, 227
BsrI ACTGG 3 cut(s) 116, 299, 319
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 2 cut(s) 309, 348
BssT1I CCWWGG 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 98
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 2 cut(s) 312, 351
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 2 cut(s) 309, 348
BstSFI CTRYAG 2 cut(s) 19, 268
BstV2I GAAGAC 1 cut(s) 112
BsuRI GGCC 1 cut(s) 96
BtsIMutI CAGTG 1 cut(s) 312
BveI ACCTGC 2 cut(s) 12, 227
Cac8I GCNNGC 1 cut(s) 98
CaiI CAGNNNCTG 1 cut(s) 97
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 3 cut(s) 94, 216, 360
CviJI RGCY 4 cut(s) 96, 166, 171, 267
CviKI_1 RGCY 4 cut(s) 96, 166, 171, 267
DpnI GATC 2 cut(s) 311, 350
DpnII GATC 2 cut(s) 309, 348
Eco130I CCWWGG 1 cut(s) 211
Eco47I GGWCC 2 cut(s) 216, 360
Eco57I CTGAAG 1 cut(s) 157
EcoO109I RGGNCCY 2 cut(s) 94, 216
EcoT14I CCWWGG 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 146
FaiI YATR 5 cut(s) 108, 227, 353, 355, 388
FalI AAGNNNNNCTT 2 cut(s) 155, 187
FaqI GGGAC 1 cut(s) 229
FbaI TGATCA 1 cut(s) 309
GlaI GCGC 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 1 cut(s) 96
HhaI GCGC 1 cut(s) 330
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HinfI GANTC 1 cut(s) 184
HphI GGTGA 1 cut(s) 299
Hpy188I TCNGA 1 cut(s) 176
Hpy188III TCNNGA 1 cut(s) 346
HpyAV CCTTC 2 cut(s) 38, 373
HpyCH4V TGCA 1 cut(s) 21
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 309
Kzo9I GATC 2 cut(s) 309, 348
LpnPI CCDG 7 cut(s) 7, 77, 110, 129, 232, 280, 300
MaeIII GTNAC 2 cut(s) 24, 301
MalI GATC 2 cut(s) 311, 350
MboI GATC 2 cut(s) 309, 348
MboII GAAGA 1 cut(s) 112
MluCI AATT 1 cut(s) 189
MnlI CCTC 5 cut(s) 111, 135, 257, 342, 347
Mva1269I GAATGC 1 cut(s) 277
NdeII GATC 2 cut(s) 309, 348
NlaIV GGNNCC 1 cut(s) 217
NmeAIII GCCGAG 1 cut(s) 147
PctI GAATGC 1 cut(s) 277
PfeI GAWTC 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 217
PspPI GGNCC 3 cut(s) 94, 216, 360
PspPPI RGGWCCY 1 cut(s) 216
PstI CTGCAG 1 cut(s) 23
PstNI CAGNNNCTG 1 cut(s) 97
Sau3AI GATC 2 cut(s) 309, 348
Sau96I GGNCC 3 cut(s) 94, 216, 360
SetI ASST 6 cut(s) 26, 64, 168, 221, 269, 376
SfcI CTRYAG 2 cut(s) 19, 268
SinI GGWCC 2 cut(s) 216, 360
Sse9I AATT 1 cut(s) 189
StyI CCWWGG 1 cut(s) 211
TaqI TCGA 1 cut(s) 347
TasI AATT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 184
TscAI CASTG 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 270
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 216, 360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.