MD12G1253700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013724
Physical Location & Seq
Forward (+)
32234931 .. 32238209
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1253700.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGGTATTGTTTCTCTCTGCAGGTTACTCGAAGAAGTCTGTGAGGGGTCAATAGGAGAAGGTGGATTGCGTTTCAAAGTGCAAAAAATACAGGGCATGCATCTTTATGCGTTTCCAATCTTCCAAAAATCGTGTTCGTGGAGGCGATTTGAGACGGATTTGAAGCTCGGCTACAGAGATTTTATTCAATTTGGTGGATTTGGAAATTTGTACCAATTTGGTTTCGATTGGAATTTCTGGGCTCATCTAATCCAACGTTTCCAGGTGAGAAAGTTGCTTAAATTTCGATTTTTAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

11.82

Weight (kDa)

9.41

Isoelectric Point (pI)

19.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 12
AclI AACGTT 1 cut(s) 256
AcsI RAATTY 3 cut(s) 205, 232, 281
AfaI GTAC 1 cut(s) 212
AgsI TTSAA 3 cut(s) 76, 163, 188
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 1 cut(s) 166
AluI AGCT 1 cut(s) 166
Alw26I GTCTC 1 cut(s) 146
ApoI RAATTY 3 cut(s) 205, 232, 281
AsuHPI GGTGA 1 cut(s) 277
BanII GRGCYC 1 cut(s) 244
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BcgI CGANNNNNNTGC 2 cut(s) 10, 44
BciT130I CCWGG 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 146
BfmI CTRYAG 2 cut(s) 19, 172
BfuAI ACCTGC 1 cut(s) 12
Bme1390I CCNGG 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 109
BseBI CCWGG 1 cut(s) 263
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 23
BspMI ACCTGC 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 98
BstMAI GTCTC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 2 cut(s) 19, 172
BveI ACCTGC 1 cut(s) 12
Cac8I GCNNGC 1 cut(s) 98
Csp6I GTAC 1 cut(s) 211
CviAII CATG 1 cut(s) 97
CviJI RGCY 3 cut(s) 166, 171, 242
CviKI_1 RGCY 3 cut(s) 166, 171, 242
CviQI GTAC 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 244
EcoRII CCWGG 1 cut(s) 261
EcoT22I ATGCAT 1 cut(s) 102
EcoT38I GRGCYC 1 cut(s) 244
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 1 cut(s) 100
FaiI YATR 2 cut(s) 98, 108
FatI CATG 1 cut(s) 96
FriOI GRGCYC 1 cut(s) 244
Hin1II CATG 1 cut(s) 100
HphI GGTGA 1 cut(s) 277
HpyAV CCTTC 1 cut(s) 53
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 3 cut(s) 21, 82, 100
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 1 cut(s) 100
LpnPI CCDG 5 cut(s) 7, 77, 223, 248, 275
LweI GCATC 1 cut(s) 109
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 1 cut(s) 24
MboII GAAGA 2 cut(s) 44, 112
MhlI GDGCHC 1 cut(s) 244
MluCI AATT 5 cut(s) 188, 205, 215, 232, 281
MmeI TCCRAC 1 cut(s) 277
MnlI CCTC 2 cut(s) 37, 135
Mph1103I ATGCAT 1 cut(s) 102
MseI TTAA 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 105
MspR9I CCNGG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 263
NlaIII CATG 1 cut(s) 100
NmeAIII GCCGAG 1 cut(s) 147
NsiI ATGCAT 1 cut(s) 102
NspI RCATGY 1 cut(s) 100
PaeI GCATGC 1 cut(s) 100
Psp1406I AACGTT 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PstI CTGCAG 1 cut(s) 23
RsaI GTAC 1 cut(s) 212
RsaNI GTAC 1 cut(s) 211
RseI CAYNNNNRTG 1 cut(s) 105
SaqAI TTAA 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 1 cut(s) 244
SetI ASST 5 cut(s) 26, 64, 168, 259, 267
SfaNI GCATC 1 cut(s) 109
SfcI CTRYAG 2 cut(s) 19, 172
SmiMI CAYNNNNRTG 1 cut(s) 105
SphI GCATGC 1 cut(s) 100
Sse9I AATT 5 cut(s) 188, 205, 215, 232, 281
StyD4I CCNGG 1 cut(s) 261
TaiI ACGT 1 cut(s) 259
TaqI TCGA 3 cut(s) 30, 225, 286
TasI AATT 5 cut(s) 188, 205, 215, 232, 281
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TspGWI ACGGA 1 cut(s) 170
XapI RAATTY 3 cut(s) 205, 232, 281
XceI RCATGY 1 cut(s) 100
Zsp2I ATGCAT 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.