pycom10g04750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0013724
Physical Location & Seq
Forward (+)
4989889 .. 4990867
979 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04750.2

Sequence Viewer

Length: 396 bp
ATGACCAGTGGGCGGACTGTTTTGCCCCTCCGTTTTATATCGATTTTCCTTGCTGCTCCCCACGTCCTCATCGTGCACCTTTTCGTTGGATTTTCTTCCCCCTATAGTGTTATACACGTGTTGCTCATCGTGGAGGGGAAGAAACATGGAAAAGCTGGCTCTTCCATTTGCTTCCACAGATTTCTCGAGCTCTCTCTCAAGCTCCGAATCGTTGGCCTCCGCGAGAAGGAAATCGCCAATTCGAAAGCTTGGGTTTCCTGTGGCTTTCTCACTAGATCGATCTTTGCCATCGCTGTTCCACCAAACTTGCATCCGCTCTTTCGAACAGGTGGTGTTCTGATTTTTCATTCGTTCTCATCACTTCTACAAGAGGTTAGTTTTCTTTTCTGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.55

Weight (kDa)

10.15

Isoelectric Point (pI)

32.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 316
AccII CGCG 1 cut(s) 222
AciI CCGC 3 cut(s) 13, 220, 314
AcvI CACGTG 1 cut(s) 118
AfiI CCNNNNNNNGG 2 cut(s) 12, 226
AflIII ACRYGT 2 cut(s) 115, 117
AjiI CACGTC 1 cut(s) 64
AluBI AGCT 4 cut(s) 155, 190, 202, 248
AluI AGCT 4 cut(s) 155, 190, 202, 248
Alw21I GWGCWC 2 cut(s) 78, 192
Alw44I GTGCAC 1 cut(s) 74
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 1 cut(s) 214
ApaLI GTGCAC 1 cut(s) 74
ApeKI GCWGC 1 cut(s) 53
AsuII TTCGAA 2 cut(s) 242, 322
AvaI CYCGRG 1 cut(s) 185
BaeGI GKGCMC 1 cut(s) 78
BanII GRGCYC 1 cut(s) 192
BbrPI CACGTG 1 cut(s) 118
Bbv12I GWGCWC 2 cut(s) 78, 192
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 296
BfaI CTAG 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 103
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 185
BmgBI CACGTC 1 cut(s) 64
BmsI GCATC 1 cut(s) 319
Bpu14I TTCGAA 2 cut(s) 242, 322
BpuEI CTTGAG 1 cut(s) 182
Bsa29I ATCGAT 2 cut(s) 41, 278
BsaAI YACGTR 1 cut(s) 118
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 226
Bse1I ACTGG 1 cut(s) 6
BseCI ATCGAT 2 cut(s) 41, 278
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 2 cut(s) 12, 226
BseNI ACTGG 1 cut(s) 6
BseSI GKGCMC 1 cut(s) 78
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 216
BshVI ATCGAT 2 cut(s) 41, 278
BsiHKAI GWGCWC 2 cut(s) 78, 192
BsiHKCI CYCGRG 1 cut(s) 185
BslI CCNNNNNNNGG 2 cut(s) 12, 226
BsnI GGCC 1 cut(s) 216
BsoBI CYCGRG 1 cut(s) 185
Bsp119I TTCGAA 2 cut(s) 242, 322
Bsp1286I GDGCHC 2 cut(s) 78, 192
Bsp143I GATC 2 cut(s) 275, 279
BspACI CCGC 3 cut(s) 13, 220, 314
BspANI GGCC 1 cut(s) 216
BspDI ATCGAT 2 cut(s) 41, 278
BspFNI CGCG 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 166
BspT104I TTCGAA 2 cut(s) 242, 322
BsrBI CCGCTC 1 cut(s) 316
BsrI ACTGG 1 cut(s) 6
BssMI GATC 2 cut(s) 275, 279
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 166
BstBAI YACGTR 1 cut(s) 118
BstBI TTCGAA 2 cut(s) 242, 322
BstC8I GCNNGC 1 cut(s) 157
BstF5I GGATG 1 cut(s) 310
BstFNI CGCG 1 cut(s) 222
BstKTI GATC 2 cut(s) 278, 282
BstMBI GATC 2 cut(s) 275, 279
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 1 cut(s) 78
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 40
Bsu15I ATCGAT 2 cut(s) 41, 278
BsuRI GGCC 1 cut(s) 216
BsuTUI ATCGAT 2 cut(s) 41, 278
BtgZI GCGATG 1 cut(s) 274
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 157
ClaI ATCGAT 2 cut(s) 41, 278
CspCI CAANNNNNGTGG 2 cut(s) 288, 323
CviAII CATG 1 cut(s) 146
CviJI RGCY 7 cut(s) 155, 159, 190, 202, 216, 248, 264
CviKI_1 RGCY 7 cut(s) 155, 159, 190, 202, 216, 248, 264
DpnI GATC 2 cut(s) 277, 281
DpnII GATC 2 cut(s) 275, 279
Eam1104I CTCTTC 1 cut(s) 166
EarI CTCTTC 1 cut(s) 166
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 190
Eco72I CACGTG 1 cut(s) 118
Eco88I CYCGRG 1 cut(s) 185
EcoICRI GAGCTC 1 cut(s) 190
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 1 cut(s) 149
FaiI YATR 4 cut(s) 38, 105, 113, 147
FatI CATG 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 1 cut(s) 297
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 273
GluI GCNGC 1 cut(s) 54
HaeIII GGCC 1 cut(s) 216
Hin1II CATG 1 cut(s) 149
HindIII AAGCTT 1 cut(s) 246
HinfI GANTC 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 76
Hpy188I TCNGA 2 cut(s) 206, 339
Hpy188III TCNNGA 1 cut(s) 185
Hpy8I GTNNAC 1 cut(s) 76
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 2 cut(s) 63, 117
HpyCH4V TGCA 3 cut(s) 76, 310, 390
HpySE526I ACGT 2 cut(s) 63, 117
Hsp92II CATG 1 cut(s) 149
Kzo9I GATC 2 cut(s) 275, 279
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 2 cut(s) 61, 207
LpnPI CCDG 4 cut(s) 19, 141, 271, 312
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 273
MaeII ACGT 2 cut(s) 63, 117
MalI GATC 2 cut(s) 277, 281
MbiI CCGCTC 1 cut(s) 316
MboI GATC 2 cut(s) 275, 279
MboII GAAGA 3 cut(s) 87, 151, 153
MhlI GDGCHC 2 cut(s) 78, 192
MluCI AATT 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 67
MnlI CCTC 5 cut(s) 38, 77, 127, 227, 364
MseI TTAA 1 cut(s) 394
MvnI CGCG 1 cut(s) 222
NdeII GATC 2 cut(s) 275, 279
NlaIII CATG 1 cut(s) 149
NspV TTCGAA 2 cut(s) 242, 322
PaeR7I CTCGAG 1 cut(s) 185
PciSI GCTCTTC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 69
PfeI GAWTC 1 cut(s) 207
PkrI GCNGC 1 cut(s) 55
PmaCI CACGTG 1 cut(s) 118
PmlI CACGTG 1 cut(s) 118
Ppu21I YACGTR 1 cut(s) 118
Psp124BI GAGCTC 1 cut(s) 192
PspCI CACGTG 1 cut(s) 118
SacI GAGCTC 1 cut(s) 192
SapI GCTCTTC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 394
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 275, 279
SduI GDGCHC 2 cut(s) 78, 192
SetI ASST 9 cut(s) 66, 81, 120, 157, 192, 204, 250, 331, 375
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 185
SfuI TTCGAA 2 cut(s) 242, 322
SlaI CTCGAG 1 cut(s) 185
SmlI CTYRAG 2 cut(s) 185, 197
SmoI CTYRAG 2 cut(s) 185, 197
Sse9I AATT 1 cut(s) 238
SsiI CCGC 3 cut(s) 13, 220, 314
SspMI CTAG 1 cut(s) 273
SstI GAGCTC 1 cut(s) 192
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 2 cut(s) 66, 120
TaqI TCGA 5 cut(s) 41, 186, 242, 278, 322
TasI AATT 1 cut(s) 238
TfiI GAWTC 1 cut(s) 207
Tru1I TTAA 1 cut(s) 394
Tru9I TTAA 1 cut(s) 394
TscAI CASTG 1 cut(s) 13
TseI GCWGC 1 cut(s) 53
TspDTI ATGAA 1 cut(s) 335
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 1 cut(s) 13
VneI GTGCAC 1 cut(s) 74
XhoI CTCGAG 1 cut(s) 185
XspI CTAG 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.