Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0001243

Physical Location & Seq

Genomic Coordinates Reverse (-)

30770336 .. 30781020

Gene Structure 10685 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1255800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGCAGCGATAACTCGACTGCAGCAAATGCAATTCCTCCACTCTCCTCTCACCCTCAAGCCTTCCGTACTCTTCCCCAACCCCAACCAACTCCGCCGCTCATGCCCCCGCACTTTCTCCACCGCTCCTATCCTCTGCTCCTCCAACTCCCCTCATCTCCCCCAGAGCTCGGTTTCTGCAGCGGGAGCGAATGCGTCAATCGTTGGGGATCTGCTCGACTACCTCAACGAGTCTTGGACTCACTTTCATGCTACCGCTGAAGCGAAAAAACAGCTAATTGCTACTGGCTTTCAATTGCTGAGTGAGAATGATGAGTGGGACCTGAAGCCTGGTGGACGCTACTTCTTTACACGGAATATGTCTTGTTTGGTTGCATTTGCCGTTGGAGAGAAGTATAGTGTTGGTAATGGTTTCCATGTGATTGCTGCACACACAGACAGTCCGTGTCTAAAAGTAAAGCCAAGATCTGCATCATCCAAGTCTGGTTATCAAATGATCAATGTGCAGACATATGGAGGTGGTCTCTGGCATACTTGGTTTGATAGAGACCTAAGTGTGGCAGGCAGGGTGATATTGAGAGGCAGCACAGGCTCCTTTTTGCATAAGCTTGTCAAAGTGAAGCCACCTCTGTTACGAATACCGACACTGGCCATTCACCTTGACAGCACAATAAACAAGGATGGATTTAAACCGAATGTAGAGACTCAACTTATTCCTTTACTGGCGTCCAAACTAGAAGAAGCATCTCTGGAGACAAAAGAGAAAAGTACCACAACATCTTCGAAAGCTAATCATCATCCATTGCTCATGCAGGTTTTATCAGATGAGGTCCATTGTAAAATCGACGACATAGTGAGTTTGGAGTTGAATATTTGTGATACCCAACCAAGCTGCCTTGGAGGTGGAAACAATGAATTTATTTTTTCAGGAAGATTAGATAATCTTGCCTCAAGTTATTGTGCGTTAAGGGCTCTCATTGATTCGTGTGAATCACCTGGTGATTTAGCAAGTGAACATTCTGTACGAATGGTTGCCTTATTTGACAATGAAGAGGTTGGTTCAGGTTCAATTCAGGGGGCTGGTGCACCAACCATGTTTCAGGCTATGAGACATATAATTAGTTGCTTAGCTGACAAGTACATTGGTGAAGGTGTTTTTGAGCGCGCAATTCGCCAATCATTTCTTGTGTCTGCAGACATGGCTCATGGAGTACATCCAAATTTCATGGACAAGCATGAAGAACACCATCGGCCACAAATGCAAAAAGGTCTAGTTATCAAGCATAATGCAAACCAGCGCTATGCTACTAGTGAAGTCACATCTTTCATTTTCAAAGAAATTGGCAAAATCCATAACCTTCCAACCCAGGAATTTGTGGTGAGAAACGATATGGGATGTGGATCTACCATAGGTCCCATACTAGCTTCGGGGGCCGGCATTCATACTGTTGATTGTGGCATTCCTCAGCTTTCTATGCACAGTGTGAGAGAGATTTGTGGGAAGGAAGATGTAGATATTGCGTACAAGTACTTCAAGGCGTTCTACCAAACTTTCTCAAGCATAGACAAGGAGCTGGATGTAGATTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004177 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0006508 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008235 GO:0008237 GO:0008238 GO:0009987 GO:0016787 GO:0019538 GO:0034641 GO:0043170 GO:0043603 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0070006 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M18

Protein Analysis

530

Amino Acids

58.11

Weight (kDa)

6.54

Isoelectric Point (pI)

37.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M18	PF02127	74 - 515	1.5e-154	Aminopeptidase I zinc metalloprotease (M18)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001243)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04710
fragaria_vesca	FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g27850 FvH4_1g30510 FvH4_1g30550 FvH4_1g30550 FvH4_1g30550 FvH4_1g30550 FvH4_1g30550 FvH4_1g30550 FvH4_1g30550 FvH4_1g30550 FvH4_7g04991 FvH4_7g05660 FvH4_7g05660 FvH4_7g05660 FvH4_7g05660 FvH4_7g05660 FvH4_7g05660 FvH4_c4g00010 FvH4_c4g00010 FvH4_c4g00010 FvH4_c4g00040 FvH4_c4g00040 FvH4_c4g00040 FvH4_c4g00040 FvH4_c4g00040 FvH4_c4g00040
malus_domestica	MD02G1255800.v1.1 MD07G1065400.v1.1
prunus_persica	Prupe.2G079500_v2.0.a1
pyrus_communis	pycom02g21670
rosa_chinensis	RchiOBHm_Chr1g0332601
rosa_laevigata	RLG00000029652 RLG00000029714
rosa_multiflora	Rmu_sc0004413.1_g000027
rosa_roxburghii	Rroxscaffold_4G00318110
rosa_rugosa	Rorug01G0105600 Rorug01G0105600
rosa_samantha	Rh1AG126700 Rh1BG096400 Rh1CG120500 Rh1DG131100
rosa_wichuraiana	Rw0G003960 Rw1G010660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	802
AccBSI	CCGCTC	2 cut(s)	99, 125
AccII	CGCG	1 cut(s)	1164
AciI	CCGC	6 cut(s)	94, 97, 109, 123, 182, 255
AclWI	GGATC	2 cut(s)	216, 1408
AcoI	YGGCCR	2 cut(s)	648, 1250
AcsI	RAATTY	3 cut(s)	914, 1219, 1370
AcuI	CTGAAG	2 cut(s)	279, 344
AcyI	GRCGYC	1 cut(s)	725
AdeI	CACNNNGTG	2 cut(s)	998, 1483
AfaI	GTAC	7 cut(s)	69, 769, 1023, 1139, 1212, 1523, 1529
AfeI	AGCGCT	1 cut(s)	1298
AfiI	CCNNNNNNNGG	2 cut(s)	169, 556
AgsI	TTSAA	5 cut(s)	293, 868, 1068, 1333, 1534
AhlI	ACTAGT	1 cut(s)	1307
AjnI	CCWGG	3 cut(s)	328, 994, 1365
AluBI	AGCT	9 cut(s)	168, 274, 607, 788, 891, 1130, 1424, 1468, 1573
AluI	AGCT	9 cut(s)	168, 274, 607, 788, 891, 1130, 1424, 1468, 1573
Alw21I	GWGCWC	2 cut(s)	170, 1087
Alw26I	GTCTC	5 cut(s)	527, 540, 695, 746, 1102
Alw44I	GTGCAC	1 cut(s)	1083
AlwI	GGATC	2 cut(s)	216, 1408
Aor51HI	AGCGCT	1 cut(s)	1298
AoxI	GGCC	3 cut(s)	648, 1250, 1431
ApaLI	GTGCAC	1 cut(s)	1083
ApeKI	GCWGC	6 cut(s)	5, 22, 179, 425, 582, 891
ApoI	RAATTY	3 cut(s)	914, 1219, 1370
AspLEI	GCGC	3 cut(s)	1164, 1166, 1299
AspS9I	GGNCC	4 cut(s)	319, 829, 1412, 1431
AsuHPI	GGTGA	7 cut(s)	43, 580, 647, 984, 1010, 1157, 1390
AsuII	TTCGAA	1 cut(s)	782
AvaII	GGWCC	3 cut(s)	319, 829, 1412
BaeGI	GKGCMC	1 cut(s)	1087
BalI	TGGCCA	1 cut(s)	650
BanII	GRGCYC	2 cut(s)	170, 973
BarI	GAAGNNNNNNTAC	2 cut(s)	1514, 1546
Bbv12I	GWGCWC	2 cut(s)	170, 1087
BbvCI	CCTCAGC	1 cut(s)	1464
BbvI	GCAGC	6 cut(s)	17, 34, 191, 412, 594, 878
BccI	CCATC	2 cut(s)	674, 1254
BceAI	ACGGC	1 cut(s)	365
BciT130I	CCWGG	3 cut(s)	330, 996, 1367
BclI	TGATCA	1 cut(s)	495
BcoDI	GTCTC	5 cut(s)	527, 540, 695, 746, 1102
BcuI	ACTAGT	1 cut(s)	1307
BfaI	CTAG	4 cut(s)	734, 1271, 1308, 1421
BfmI	CTRYAG	3 cut(s)	20, 177, 1191
BfoI	RGCGCY	1 cut(s)	1300
BfuAI	ACCTGC	1 cut(s)	802
BglII	AGATCT	1 cut(s)	464
BisI	GCNGC	7 cut(s)	6, 23, 97, 180, 426, 583, 892
BlpI	GCTNAGC	1 cut(s)	1126
BlsI	GCNGC	7 cut(s)	7, 24, 98, 181, 427, 584, 893
BmcAI	AGTACT	1 cut(s)	1529
Bme1390I	CCNGG	3 cut(s)	330, 996, 1367
Bme18I	GGWCC	3 cut(s)	319, 829, 1412
BmgT120I	GGNCC	4 cut(s)	319, 829, 1412, 1431
BmiI	GGNNCC	4 cut(s)	320, 592, 1414, 1432
BmrFI	CCNGG	3 cut(s)	330, 996, 1367
BmsI	GCATC	2 cut(s)	479, 752
BplI	GAGNNNNNCTC	2 cut(s)	507, 539
BpmI	CTGGAG	1 cut(s)	770
Bpu10I	CCTNAGC	1 cut(s)	1464
Bpu1102I	GCTNAGC	1 cut(s)	1126
Bpu14I	TTCGAA	1 cut(s)	782
BpuEI	CTTGAG	3 cut(s)	41, 934, 1540
BsaBI	GATNNNNATC	2 cut(s)	469, 1399
BsaHI	GRCGYC	1 cut(s)	725
BsaI	GGTCTC	2 cut(s)	527, 540
BsaJI	CCNNGG	2 cut(s)	895, 1365
Bsc4I	CCNNNNNNNGG	2 cut(s)	169, 556
Bse118I	RCCGGY	1 cut(s)	1433
Bse1I	ACTGG	3 cut(s)	289, 651, 726
Bse3DI	GCAATG	1 cut(s)	800
Bse8I	GATNNNNATC	2 cut(s)	469, 1399
BseBI	CCWGG	3 cut(s)	330, 996, 1367
BseDI	CCNNGG	2 cut(s)	895, 1365
BseGI	GGATG	6 cut(s)	473, 685, 796, 1213, 1400, 1582
BseJI	GATNNNNATC	2 cut(s)	469, 1399
BseLI	CCNNNNNNNGG	2 cut(s)	169, 556
BseMI	GCAATG	1 cut(s)	800
BseMII	CTCAG	2 cut(s)	290, 1478
BseNI	ACTGG	3 cut(s)	289, 651, 726
BsePI	GCGCGC	1 cut(s)	1162
BseRI	GAGGAG	2 cut(s)	36, 130
BseSI	GKGCMC	1 cut(s)	1087
BseXI	GCAGC	6 cut(s)	17, 34, 191, 412, 594, 878
BsgI	GTGCAG	2 cut(s)	411, 524
Bsh1236I	CGCG	1 cut(s)	1164
BshFI	GGCC	3 cut(s)	650, 1252, 1433
BsiHKAI	GWGCWC	2 cut(s)	170, 1087
BsiSI	CCGG	1 cut(s)	1434
BslFI	GGGAC	2 cut(s)	332, 1398
BslI	CCNNNNNNNGG	2 cut(s)	169, 556
BsmAI	GTCTC	5 cut(s)	527, 540, 695, 746, 1102
BsmFI	GGGAC	2 cut(s)	332, 1398
BsmI	GAATGC	3 cut(s)	196, 1437, 1458
BsnI	GGCC	3 cut(s)	650, 1252, 1433
Bso31I	GGTCTC	2 cut(s)	527, 540
Bsp119I	TTCGAA	1 cut(s)	782
Bsp1286I	GDGCHC	3 cut(s)	170, 973, 1087
Bsp143I	GATC	4 cut(s)	208, 464, 495, 1400
Bsp1720I	GCTNAGC	1 cut(s)	1126
BspACI	CCGC	6 cut(s)	94, 97, 109, 123, 182, 255
BspANI	GGCC	3 cut(s)	650, 1252, 1433
BspCNI	CTCAG	2 cut(s)	291, 1477
BspFNI	CGCG	1 cut(s)	1164
BspLI	GGNNCC	4 cut(s)	320, 592, 1414, 1432
BspMAI	CTGCAG	3 cut(s)	24, 181, 1195
BspMI	ACCTGC	1 cut(s)	802
BspPI	GGATC	2 cut(s)	216, 1408
BspT104I	TTCGAA	1 cut(s)	782
BspTNI	GGTCTC	2 cut(s)	527, 540
BsrBI	CCGCTC	2 cut(s)	99, 125
BsrDI	GCAATG	1 cut(s)	800
BsrFI	RCCGGY	1 cut(s)	1433
BsrI	ACTGG	3 cut(s)	289, 651, 726
BssAI	RCCGGY	1 cut(s)	1433
BssECI	CCNNGG	2 cut(s)	895, 1365
BssHII	GCGCGC	1 cut(s)	1162
BssMI	GATC	4 cut(s)	208, 464, 495, 1400
BssNI	GRCGYC	1 cut(s)	725
BssT1I	CCWWGG	1 cut(s)	895
Bst2UI	CCWGG	3 cut(s)	330, 996, 1367
Bst4CI	ACNGT	3 cut(s)	440, 1447, 1481
Bst6I	CTCTTC	2 cut(s)	77, 1044
BstACI	GRCGYC	1 cut(s)	725
BstAPI	GCANNNNNTGC	1 cut(s)	28
BstBI	TTCGAA	1 cut(s)	782
BstC8I	GCNNGC	3 cut(s)	562, 1164, 1435
BstDEI	CTNAG	4 cut(s)	299, 551, 1126, 1464
BstF5I	GGATG	6 cut(s)	473, 685, 796, 1213, 1400, 1582
BstFNI	CGCG	1 cut(s)	1164
BstH2I	RGCGCY	1 cut(s)	1300
BstHHI	GCGC	3 cut(s)	1164, 1166, 1299
BstKTI	GATC	4 cut(s)	211, 467, 498, 1403
BstMAI	GTCTC	5 cut(s)	527, 540, 695, 746, 1102
BstMBI	GATC	4 cut(s)	208, 464, 495, 1400
BstNI	CCWGG	3 cut(s)	330, 996, 1367
BstSCI	CCNGG	3 cut(s)	328, 994, 1365
BstSFI	CTRYAG	3 cut(s)	20, 177, 1191
BstSLI	GKGCMC	1 cut(s)	1087
BstUI	CGCG	1 cut(s)	1164
BstV1I	GCAGC	6 cut(s)	17, 34, 191, 412, 594, 878
BstX2I	RGATCY	3 cut(s)	208, 464, 1400
BstYI	RGATCY	3 cut(s)	208, 464, 1400
BsuRI	GGCC	3 cut(s)	650, 1252, 1433
BtsCI	GGATG	6 cut(s)	473, 685, 796, 1213, 1400, 1582
BtsIMutI	CAGTG	2 cut(s)	644, 1486
BveI	ACCTGC	1 cut(s)	802
Cac8I	GCNNGC	3 cut(s)	562, 1164, 1435
CfoI	GCGC	3 cut(s)	1164, 1166, 1299
Cfr10I	RCCGGY	1 cut(s)	1433
Cfr13I	GGNCC	4 cut(s)	319, 829, 1412, 1431
CseI	GACGC	3 cut(s)	183, 345, 714
CsiI	ACCWGGT	1 cut(s)	994
Csp6I	GTAC	7 cut(s)	68, 768, 1022, 1138, 1211, 1522, 1528
CviAII	CATG	9 cut(s)	102, 248, 416, 808, 1093, 1198, 1205, 1225, 1235
CviQI	GTAC	7 cut(s)	68, 768, 1022, 1138, 1211, 1522, 1528
DdeI	CTNAG	4 cut(s)	299, 551, 1126, 1464
DpnI	GATC	4 cut(s)	210, 466, 497, 1402
DpnII	GATC	4 cut(s)	208, 464, 495, 1400
DraI	TTTAAA	1 cut(s)	688
DraIII	CACNNNGTG	2 cut(s)	998, 1483
EaeI	YGGCCR	2 cut(s)	648, 1250
Eam1104I	CTCTTC	2 cut(s)	77, 1044
EarI	CTCTTC	2 cut(s)	77, 1044
EciI	GGCGGA	1 cut(s)	83
Ecl136II	GAGCTC	1 cut(s)	168
Eco130I	CCWWGG	1 cut(s)	895
Eco24I	GRGCYC	2 cut(s)	170, 973
Eco31I	GGTCTC	2 cut(s)	527, 540
Eco47I	GGWCC	3 cut(s)	319, 829, 1412
Eco47III	AGCGCT	1 cut(s)	1298
Eco53kI	GAGCTC	1 cut(s)	168
Eco57I	CTGAAG	2 cut(s)	279, 344
EcoICRI	GAGCTC	1 cut(s)	168
EcoO109I	RGGNCCY	2 cut(s)	319, 1412
EcoRII	CCWGG	3 cut(s)	328, 994, 1365
EcoT14I	CCWWGG	1 cut(s)	895
EcoT38I	GRGCYC	2 cut(s)	170, 973
ErhI	CCWWGG	1 cut(s)	895
FaeI	CATG	9 cut(s)	105, 251, 419, 811, 1096, 1201, 1208, 1228, 1238
FaqI	GGGAC	2 cut(s)	332, 1398
FatI	CATG	9 cut(s)	101, 247, 415, 807, 1092, 1197, 1204, 1224, 1234
FauI	CCCGC	2 cut(s)	116, 175
FauNDI	CATATG	1 cut(s)	511
FbaI	TGATCA	1 cut(s)	495
Fnu4HI	GCNGC	7 cut(s)	6, 23, 97, 180, 426, 583, 892
FokI	GGATG	5 cut(s)	460, 692, 783, 1200, 1407
FriOI	GRGCYC	2 cut(s)	170, 973
Fsp4HI	GCNGC	7 cut(s)	6, 23, 97, 180, 426, 583, 892
FspBI	CTAG	4 cut(s)	734, 1271, 1308, 1421
GlaI	GCGC	3 cut(s)	1163, 1165, 1298
GluI	GCNGC	7 cut(s)	6, 23, 97, 180, 426, 583, 892
GsuI	CTGGAG	1 cut(s)	770
HaeII	RGCGCY	1 cut(s)	1300
HaeIII	GGCC	3 cut(s)	650, 1252, 1433
HapII	CCGG	1 cut(s)	1434
HgaI	GACGC	3 cut(s)	183, 345, 714
HhaI	GCGC	3 cut(s)	1164, 1166, 1299
Hin1I	GRCGYC	1 cut(s)	725
Hin1II	CATG	9 cut(s)	105, 251, 419, 811, 1096, 1201, 1208, 1228, 1238
Hin6I	GCGC	3 cut(s)	1162, 1164, 1297
HinP1I	GCGC	3 cut(s)	1162, 1164, 1297
HindIII	AAGCTT	1 cut(s)	605
HinfI	GANTC	6 cut(s)	230, 238, 703, 980, 989, 1583
HpaII	CCGG	1 cut(s)	1434
HphI	GGTGA	7 cut(s)	43, 580, 647, 984, 1010, 1157, 1390
Hpy166II	GTNNAC	3 cut(s)	335, 1013, 1085
Hpy188I	TCNGA	1 cut(s)	823
Hpy188III	TCNNGA	2 cut(s)	749, 927
Hpy8I	GTNNAC	3 cut(s)	335, 1013, 1085
Hpy99I	CGWCG	1 cut(s)	848
HpyAV	CCTTC	4 cut(s)	72, 1142, 1367, 1495
HpyCH4III	ACNGT	3 cut(s)	440, 1447, 1481
HpyF3I	CTNAG	4 cut(s)	299, 551, 1126, 1464
Hsp92I	GRCGYC	1 cut(s)	725
Hsp92II	CATG	9 cut(s)	105, 251, 419, 811, 1096, 1201, 1208, 1228, 1238
HspAI	GCGC	3 cut(s)	1162, 1164, 1297
KroI	GCCGGC	1 cut(s)	1433
KroNI	GCCGGC	1 cut(s)	1435
Ksp22I	TGATCA	1 cut(s)	495
Kzo9I	GATC	4 cut(s)	208, 464, 495, 1400
LmnI	GCTCC	5 cut(s)	130, 143, 185, 596, 1570
Lsp1109I	GCAGC	6 cut(s)	17, 34, 191, 412, 594, 878
LweI	GCATC	2 cut(s)	479, 752
MabI	ACCWGGT	1 cut(s)	994
MaeI	CTAG	4 cut(s)	734, 1271, 1308, 1421
MaeIII	GTNAC	2 cut(s)	630, 1315
MalI	GATC	4 cut(s)	210, 466, 497, 1402
MbiI	CCGCTC	2 cut(s)	99, 125
MboI	GATC	4 cut(s)	208, 464, 495, 1400
MboII	GAAGA	7 cut(s)	64, 749, 771, 942, 1061, 1250, 1517
MfeI	CAATTG	1 cut(s)	293
MflI	RGATCY	3 cut(s)	208, 464, 1400
MhlI	GDGCHC	3 cut(s)	170, 973, 1087
MlsI	TGGCCA	1 cut(s)	650
MluNI	TGGCCA	1 cut(s)	650
MlyI	GAGTC	3 cut(s)	232, 239, 697
MmeI	TCCRAC	3 cut(s)	168, 364, 1385
Mox20I	TGGCCA	1 cut(s)	650
MroNI	GCCGGC	1 cut(s)	1433
MscI	TGGCCA	1 cut(s)	650
MseI	TTAA	2 cut(s)	687, 965
MslI	CAYNNNNRTG	1 cut(s)	246
Msp20I	TGGCCA	1 cut(s)	650
MspA1I	CMGCKG	2 cut(s)	182, 257
MspI	CCGG	1 cut(s)	1434
MspR9I	CCNGG	3 cut(s)	330, 996, 1367
MunI	CAATTG	1 cut(s)	293
Mva1269I	GAATGC	3 cut(s)	196, 1437, 1458
MvaI	CCWGG	3 cut(s)	330, 996, 1367
MvnI	CGCG	1 cut(s)	1164
NaeI	GCCGGC	1 cut(s)	1435
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	4 cut(s)	208, 464, 495, 1400
NgoMIV	GCCGGC	1 cut(s)	1433
NlaIII	CATG	9 cut(s)	105, 251, 419, 811, 1096, 1201, 1208, 1228, 1238
NlaIV	GGNNCC	4 cut(s)	320, 592, 1414, 1432
NmuCI	GTSAC	1 cut(s)	1315
NspV	TTCGAA	1 cut(s)	782
PauI	GCGCGC	1 cut(s)	1162
PctI	GAATGC	3 cut(s)	196, 1437, 1458
PdiI	GCCGGC	1 cut(s)	1435
PfeI	GAWTC	3 cut(s)	980, 989, 1583
PkrI	GCNGC	7 cut(s)	7, 24, 98, 181, 427, 584, 893
PleI	GAGTC	3 cut(s)	232, 238, 697
PpsI	GAGTC	3 cut(s)	232, 238, 697
PpuMI	RGGWCCY	2 cut(s)	319, 1412
Psp124BI	GAGCTC	1 cut(s)	170
Psp5II	RGGWCCY	2 cut(s)	319, 1412
Psp6I	CCWGG	3 cut(s)	328, 994, 1365
PspGI	CCWGG	3 cut(s)	328, 994, 1365
PspN4I	GGNNCC	4 cut(s)	320, 592, 1414, 1432
PspPI	GGNCC	4 cut(s)	319, 829, 1412, 1431
PspPPI	RGGWCCY	2 cut(s)	319, 1412
PsrI	GAACNNNNNNTAC	2 cut(s)	1005, 1037
PstI	CTGCAG	3 cut(s)	24, 181, 1195
PsuI	RGATCY	3 cut(s)	208, 464, 1400
PteI	GCGCGC	1 cut(s)	1162
RsaI	GTAC	7 cut(s)	69, 769, 1023, 1139, 1212, 1523, 1529
RsaNI	GTAC	7 cut(s)	68, 768, 1022, 1138, 1211, 1522, 1528
RseI	CAYNNNNRTG	1 cut(s)	246
SacI	GAGCTC	1 cut(s)	170
SaqAI	TTAA	2 cut(s)	687, 965
SatI	GCNGC	7 cut(s)	6, 23, 97, 180, 426, 583, 892
Sau3AI	GATC	4 cut(s)	208, 464, 495, 1400
Sau96I	GGNCC	4 cut(s)	319, 829, 1412, 1431
ScaI	AGTACT	1 cut(s)	1529
SchI	GAGTC	3 cut(s)	232, 239, 697
ScrFI	CCNGG	3 cut(s)	330, 996, 1367
SduI	GDGCHC	3 cut(s)	170, 973, 1087
SexAI	ACCWGGT	1 cut(s)	994
SfaNI	GCATC	2 cut(s)	479, 752
SfcI	CTRYAG	3 cut(s)	20, 177, 1191
SfuI	TTCGAA	1 cut(s)	782
SinI	GGWCC	3 cut(s)	319, 829, 1412
SmiMI	CAYNNNNRTG	1 cut(s)	246
SmlI	CTYRAG	3 cut(s)	56, 949, 1555
SmoI	CTYRAG	3 cut(s)	56, 949, 1555
SpeI	ACTAGT	1 cut(s)	1307
SsiI	CCGC	6 cut(s)	94, 97, 109, 123, 182, 255
SspI	AATATT	1 cut(s)	871
SspMI	CTAG	4 cut(s)	734, 1271, 1308, 1421
SstI	GAGCTC	1 cut(s)	170
StyD4I	CCNGG	3 cut(s)	328, 994, 1365
StyI	CCWWGG	1 cut(s)	895
TaaI	ACNGT	3 cut(s)	440, 1447, 1481
TaqI	TCGA	4 cut(s)	16, 216, 782, 843
TatI	WGTACW	3 cut(s)	1137, 1210, 1527
TauI	GCSGC	1 cut(s)	99
TfiI	GAWTC	3 cut(s)	980, 989, 1583
Tru1I	TTAA	2 cut(s)	687, 965
Tru9I	TTAA	2 cut(s)	687, 965
TscAI	CASTG	2 cut(s)	651, 1486
TseFI	GTSAC	1 cut(s)	1315
TseI	GCWGC	6 cut(s)	5, 22, 179, 425, 582, 891
Tsp45I	GTSAC	1 cut(s)	1315
TspDTI	ATGAA	7 cut(s)	236, 927, 1062, 1213, 1251, 1315, 1430
TspGWI	ACGGA	3 cut(s)	55, 367, 432
TspRI	CASTG	2 cut(s)	651, 1486
VneI	GTGCAC	1 cut(s)	1083
VpaK11BI	GGWCC	3 cut(s)	319, 829, 1412
XapI	RAATTY	3 cut(s)	914, 1219, 1370
XspI	CTAG	4 cut(s)	734, 1271, 1308, 1421
ZrmI	AGTACT	1 cut(s)	1529

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.