Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003422

Physical Location & Seq

Genomic Coordinates Reverse (-)

31241903 .. 31246657

Gene Structure 4755 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1259000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGAGATTAGTGCCCCAGTCCCCCATGGCCCCTCCTCCTCTTTCTCAAGCCCCCGGGCCTACCTCCGAGCCCTGGCTGACACACCTACCCGCCTCGCTCGCCGGGCCAGTTCCGTCTCCACTTCCTACGACGAAATGAGTGCCGTCCGAGCTCGCTCCGGGCCCCACATGCAGAAGACGCTCCGCTGGTTCGACCTCGTCGGATTGGGCCTCGGCGGGATGGTCGGTGCTGGCGTCTTTGTCACCACCGGTCGTGCCAGTCGCCTCTACGCCGGCCCGGCTATCGTCATCTCCTACGCCATTGCCGGATTCTGCGCTCTTCTCTCCGCCTTCTGCTACACTGAGTTCGCCGTCGACATGCCCGTTGCTGGCGGCGCCTTTAGCTACCTCCGCGTCACCTTCGGTGAATTCGCGGCGTTTTTAACAGGAGCGAACCTGGTGATGGACTACGTAATGTCGAACGCCGCCGTCGCAAGGGGCTTCACCGCCTACATGGGCTCTGCAATAGGCGTCCCCGCCGCCAAATGGAGGTTCACCGTCTCCGGACTCCCCAACGGATTCGATCAGATCGACTTCGTTGCCGTCGTCGTCGTTTTAACCATTACGCTCATCATCTGCTACAGTACGAAGGAGAGCTCTGTTTTGAACATGGTGCTGACGGCGGTGCATATAATCTTCATCGCATTTGTTATAGTCGCTGGATTCTGCAAAGGGGACTGGAACAACTTCACGCACCCGGCCGACCCGGCTCAGCCATCCGGGTTTTTTCCTTACGGGGTTTCGGGCGTGTTCAATGGGGCAAGTATGGTTTACCTCAGCTACATCGGGTACGACGCTGTATCAACGATGGCGGAAGAGGTGAAGAATCCGGTTAAGGACATCCCAATCGGCGTGTCGGGCTCCGTCTTGCTCGTCACCGTCCTCTACTGCTTAATGGCGGCGTCCATGTCGAAGCTCCTCCCGTACGACGCGATCGACCCGGAGGCTCCGTTTTCGGCGGCGTTCAGAGGGAAGTGGGAGTCGAACGTGATCGGAGTGGGGGCAAGTTTTGGAATTCTAACGTCACTGCTTGTATCCATGCTGGGCCAGGCGCGTTACATGTGTGTCATCGGACGGTCCAGGGTTGTCCCTGCTTGGTTCGCTAGGGTCCACCCTAAGACATCCACGCCTGTCAACGCCTCCGCTTTTCTCGGTATCTTGACAGCCGCAATTGCGCTTTTCACGGATCTGAATGTCCTTCTCAACCTTGTATCCATCGGGACGCTATTTGTTTTCTACATGGTGGCCAACGCTGTGATTTTTAGGCGCTATGTCGTGGTGGGGACGACCAATCCACGACCTACATTATCATTCCTGTGCTCATTATCCCTCACCTCTATCATCTTCGTCTTCATCTGGCATTTCGCACCACCAGGGAAGCTGAAAGCCTTCATGCTTGTTGTCACCGCAGCAATTGCGGTTTCAATGCTGCAAATTTTCCATTACCAGGTTCCACAAGTGAGAAAGCCGGAATATTGGGGCGTCCCCTTGATGCCATGGATGCCATCCATCTCCATCTTCTTGAACATATTTTTGTTGGGGTCCATGGACAAGCCATCCTATGTGAGGTTCGGGGTCTTTTCGGGAGTTACGGTGCTCGTGTATGTGTTCTATAGCGTTCATGCTAGCTTTGATGCAGAAGGAAGTGGACTTCTCAGTCAAAAGAACGGTGAATTTCACAAAGAATCAGCTCAAGAAAGAAATGATCCAAGTCTTGGAGTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0009605 GO:0009607 GO:0009624 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0022857 GO:0034220 GO:0043207 GO:0044464 GO:0046942 GO:0046943 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0071702 GO:0071705 GO:0071944 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

588

Amino Acids

63.25

Weight (kDa)

9.0

Isoelectric Point (pI)

38.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	61 - 475	9.9e-54	Amino acid permease
AA_permease	PF00324	69 - 465	2e-31	Amino acid permease
AA_permease_C	PF13906	506 - 556	3.1e-14	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003422)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G10600 AT3G10600 AT5G04770 AT5G04770 AT5G04770
fragaria_vesca	FvH4_5g20380 FvH4_5g20380 FvH4_7g05050
malus_domestica	MD02G1259000.v1.1 MD04G1055400.v1.1 MD06G1047000.v1.1
prunus_persica	Prupe.2G071100_v2.0.a1 Prupe.2G071100_v2.0.a1 Prupe.5G055200_v2.0.a1
pyrus_communis	pycom02g22020 pycom04g04840
rosa_chinensis	RchiOBHm_Chr1g0331741 RchiOBHm_Chr7g0205641
rosa_laevigata	RLG00000003368 RLG00000029718
rosa_multiflora	Rmu_sc0006124.1_g000001 Rmu_ssc0000089.1_g000013
rosa_roxburghii	Rroxscaffold_3G00252110 Rroxscaffold_4G00318820
rosa_rugosa	Rorug01G0095900 Rorug01G0096000 Rorug07G0092300 Rorug07G0092300
rosa_samantha	Rh1BG091400 Rh1DG125300 Rh7AG223200 Rh7BG218200 Rh7CG236500 Rh7DG230200
rosa_wichuraiana	Rw1G009690 Rw7G019250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1695
AccB1I	GGYRCC	1 cut(s)	374
AccB7I	CCANNNNNTGG	1 cut(s)	1754
AccI	GTMKAC	1 cut(s)	354
AccII	CGCG	4 cut(s)	393, 413, 971, 1093
AccIII	TCCGGA	1 cut(s)	542
AclWI	GGATC	2 cut(s)	1233, 1739
AcoI	YGGCCR	2 cut(s)	738, 1284
AcsI	RAATTY	4 cut(s)	407, 1053, 1473, 1712
AcyI	GRCGYC	5 cut(s)	234, 375, 510, 941, 1521
AfaI	GTAC	3 cut(s)	625, 830, 965
AfiI	CCNNNNNNNGG	9 cut(s)	73, 368, 442, 474, 525, 528, 1486, 1605, 1754
AflIII	ACRYGT	1 cut(s)	1098
AgeI	ACCGGT	1 cut(s)	248
AgsI	TTSAA	4 cut(s)	646, 793, 1464, 1564
AjnI	CCWGG	6 cut(s)	72, 435, 1086, 1118, 1411, 1485
AjuI	GAANNNNNNNTTGG	2 cut(s)	515, 547
AluBI	AGCT	8 cut(s)	152, 384, 636, 819, 955, 1420, 1668, 1730
AluI	AGCT	8 cut(s)	152, 384, 636, 819, 955, 1420, 1668, 1730
Alw21I	GWGCWC	4 cut(s)	154, 638, 1361, 1638
Alw26I	GTCTC	2 cut(s)	121, 544
AlwI	GGATC	2 cut(s)	1233, 1739
Ama87I	CYCGRG	1 cut(s)	54
Aor13HI	TCCGGA	1 cut(s)	542
AoxI	GGCC	9 cut(s)	28, 57, 105, 161, 208, 274, 738, 1084, 1284
ApaI	GGGCCC	1 cut(s)	165
ApeKI	GCWGC	2 cut(s)	1448, 1468
ApoI	RAATTY	4 cut(s)	407, 1053, 1473, 1712
AsiGI	ACCGGT	1 cut(s)	248
Asp700I	GAANNNNTTC	2 cut(s)	725, 1427
AspLEI	GCGC	5 cut(s)	317, 377, 1093, 1216, 1308
AsuC2I	CCSGG	9 cut(s)	55, 56, 104, 160, 278, 737, 746, 760, 980
AsuNHI	GCTAGC	1 cut(s)	1664
AvaI	CYCGRG	1 cut(s)	54
AvaII	GGWCC	3 cut(s)	1116, 1147, 1581
BaeGI	GKGCMC	2 cut(s)	16, 165
BalI	TGGCCA	1 cut(s)	1286
BanI	GGYRCC	1 cut(s)	374
BanII	GRGCYC	6 cut(s)	73, 154, 165, 500, 638, 902
BauI	CACGAG	1 cut(s)	1637
BbsI	GAAGAC	2 cut(s)	182, 1381
Bbv12I	GWGCWC	4 cut(s)	154, 638, 1361, 1638
BbvCI	CCTCAGC	1 cut(s)	815
BbvI	GCAGC	2 cut(s)	1455, 1460
BccI	CCATC	9 cut(s)	214, 436, 763, 841, 1262, 1552, 1556, 1562, 1603
BceAI	ACGGC	5 cut(s)	128, 335, 452, 566, 675
BcgI	CGANNNNNNTGC	4 cut(s)	122, 156, 560, 594
BciT130I	CCWGG	6 cut(s)	74, 437, 1088, 1120, 1413, 1487
BciVI	GTATCC	2 cut(s)	1084, 1261
BcnI	CCSGG	9 cut(s)	55, 56, 104, 160, 278, 737, 746, 760, 980
BcoDI	GTCTC	2 cut(s)	121, 544
BfaI	CTAG	2 cut(s)	1143, 1665
BfmI	CTRYAG	2 cut(s)	619, 1651
BfoI	RGCGCY	2 cut(s)	378, 1309
BfuI	GTATCC	2 cut(s)	1084, 1261
BglI	GCCNNNNNGGC	2 cut(s)	278, 746
BisI	GCNGC	9 cut(s)	373, 414, 465, 519, 939, 999, 1206, 1449, 1469
BlpI	GCTNAGC	1 cut(s)	750
BlsI	GCNGC	9 cut(s)	374, 415, 466, 520, 940, 1000, 1207, 1450, 1470
Bme18I	GGWCC	3 cut(s)	1116, 1147, 1581
BmeT110I	CYCGRG	1 cut(s)	54
BmiI	GGNNCC	9 cut(s)	31, 163, 164, 376, 901, 987, 1148, 1491, 1582
BmrI	ACTGGG	1 cut(s)	11
BmsI	GCATC	3 cut(s)	1521, 1530, 1663
BmtI	GCTAGC	1 cut(s)	1668
BmuI	ACTGGG	1 cut(s)	11
BpiI	GAAGAC	2 cut(s)	182, 1381
Bpu10I	CCTNAGC	1 cut(s)	815
Bpu1102I	GCTNAGC	1 cut(s)	750
BpuEI	CTTGAG	2 cut(s)	31, 1716
BpuMI	CCSGG	9 cut(s)	55, 56, 104, 160, 278, 737, 746, 760, 980
BsaAI	YACGTR	1 cut(s)	451
BsaHI	GRCGYC	5 cut(s)	234, 375, 510, 941, 1521
BsaJI	CCNNGG	9 cut(s)	25, 53, 54, 72, 211, 1119, 1412, 1535, 1584
BsaWI	WCCGGW	3 cut(s)	248, 542, 868
BsaXI	ACNNNNNCTCC	4 cut(s)	974, 1004, 1617, 1647
Bsc4I	CCNNNNNNNGG	9 cut(s)	73, 368, 442, 474, 525, 528, 1486, 1605, 1754
Bse118I	RCCGGY	2 cut(s)	248, 272
Bse1I	ACTGG	4 cut(s)	17, 108, 258, 722
Bse3DI	GCAATG	1 cut(s)	300
BseAI	TCCGGA	1 cut(s)	542
BseBI	CCWGG	6 cut(s)	74, 437, 1088, 1120, 1413, 1487
BseDI	CCNNGG	9 cut(s)	25, 53, 54, 72, 211, 1119, 1412, 1535, 1584
BseGI	GGATG	7 cut(s)	225, 755, 879, 1160, 1544, 1545, 1595
BseLI	CCNNNNNNNGG	9 cut(s)	73, 368, 442, 474, 525, 528, 1486, 1605, 1754
BseMI	GCAATG	1 cut(s)	300
BseMII	CTCAG	4 cut(s)	333, 764, 829, 1708
BseNI	ACTGG	4 cut(s)	17, 108, 258, 722
BseRI	GAGGAG	3 cut(s)	25, 28, 947
BseSI	GKGCMC	2 cut(s)	16, 165
BseX3I	CGGCCG	1 cut(s)	738
BseXI	GCAGC	2 cut(s)	1455, 1460
BseYI	CCCAGC	1 cut(s)	1081
Bsh1236I	CGCG	4 cut(s)	393, 413, 971, 1093
Bsh1285I	CGRYCG	3 cut(s)	253, 741, 975
BshFI	GGCC	9 cut(s)	30, 59, 107, 163, 210, 276, 740, 1086, 1286
BshNI	GGYRCC	1 cut(s)	374
BshTI	ACCGGT	1 cut(s)	248
BsiEI	CGRYCG	3 cut(s)	253, 741, 975
BsiHKAI	GWGCWC	4 cut(s)	154, 638, 1361, 1638
BsiHKCI	CYCGRG	1 cut(s)	54
BsiWI	CGTACG	1 cut(s)	963
BslFI	GGGAC	7 cut(s)	5, 497, 728, 1112, 1273, 1336, 1508
BslI	CCNNNNNNNGG	9 cut(s)	73, 368, 442, 474, 525, 528, 1486, 1605, 1754
BsmAI	GTCTC	2 cut(s)	121, 544
BsmBI	CGTCTC	2 cut(s)	121, 544
BsmFI	GGGAC	7 cut(s)	5, 497, 728, 1112, 1273, 1336, 1508
BsnI	GGCC	9 cut(s)	30, 59, 107, 163, 210, 276, 740, 1086, 1286
BsoBI	CYCGRG	1 cut(s)	54
Bsp120I	GGGCCC	1 cut(s)	161
Bsp1286I	GDGCHC	9 cut(s)	16, 73, 154, 165, 500, 638, 902, 1361, 1638
Bsp13I	TCCGGA	1 cut(s)	542
Bsp143I	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
Bsp1720I	GCTNAGC	1 cut(s)	750
Bsp19I	CCATGG	3 cut(s)	25, 1535, 1584
BspANI	GGCC	9 cut(s)	30, 59, 107, 163, 210, 276, 740, 1086, 1286
BspCNI	CTCAG	4 cut(s)	334, 763, 828, 1707
BspEI	TCCGGA	1 cut(s)	542
BspFNI	CGCG	4 cut(s)	393, 413, 971, 1093
BspLI	GGNNCC	9 cut(s)	31, 163, 164, 376, 901, 987, 1148, 1491, 1582
BspOI	GCTAGC	1 cut(s)	1668
BspPI	GGATC	2 cut(s)	1233, 1739
BspQI	GCTCTTC	1 cut(s)	324
BspT107I	GGYRCC	1 cut(s)	374
BsrDI	GCAATG	1 cut(s)	300
BsrFI	RCCGGY	2 cut(s)	248, 272
BsrI	ACTGG	4 cut(s)	17, 108, 258, 722
BssAI	RCCGGY	2 cut(s)	248, 272
BssECI	CCNNGG	9 cut(s)	25, 53, 54, 72, 211, 1119, 1412, 1535, 1584
BssMI	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
BssNI	GRCGYC	5 cut(s)	234, 375, 510, 941, 1521
BssSI	CACGAG	1 cut(s)	1637
BssT1I	CCWWGG	3 cut(s)	25, 1535, 1584
Bst2BI	CACGAG	1 cut(s)	1637
Bst2UI	CCWGG	6 cut(s)	74, 437, 1088, 1120, 1413, 1487
Bst4CI	ACNGT	6 cut(s)	538, 623, 919, 1116, 1633, 1709
Bst6I	CTCTTC	2 cut(s)	324, 849
BstACI	GRCGYC	5 cut(s)	234, 375, 510, 941, 1521
BstAPI	GCANNNNNTGC	1 cut(s)	1454
BstBAI	YACGTR	1 cut(s)	451
BstC8I	GCNNGC	6 cut(s)	100, 154, 232, 274, 370, 1666
BstDEI	CTNAG	5 cut(s)	342, 750, 815, 1155, 1694
BstDSI	CCRYGG	3 cut(s)	25, 1535, 1584
BstENI	CCTNNNNNAGG	1 cut(s)	1603
BstF5I	GGATG	7 cut(s)	225, 755, 879, 1160, 1544, 1545, 1595
BstFNI	CGCG	4 cut(s)	393, 413, 971, 1093
BstH2I	RGCGCY	2 cut(s)	378, 1309
BstHHI	GCGC	5 cut(s)	317, 377, 1093, 1216, 1308
BstKTI	GATC	6 cut(s)	565, 570, 975, 1032, 1228, 1747
BstMAI	GTCTC	2 cut(s)	121, 544
BstMBI	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
BstMCI	CGRYCG	3 cut(s)	253, 741, 975
BstNI	CCWGG	6 cut(s)	74, 437, 1088, 1120, 1413, 1487
BstNSI	RCATGY	3 cut(s)	172, 361, 1102
BstSFI	CTRYAG	2 cut(s)	619, 1651
BstSLI	GKGCMC	2 cut(s)	16, 165
BstSNI	TACGTA	1 cut(s)	451
BstUI	CGCG	4 cut(s)	393, 413, 971, 1093
BstV1I	GCAGC	2 cut(s)	1455, 1460
BstV2I	GAAGAC	2 cut(s)	182, 1381
BstX2I	RGATCY	1 cut(s)	1225
BstYI	RGATCY	1 cut(s)	1225
BstZI	CGGCCG	1 cut(s)	738
BsuI	GTATCC	2 cut(s)	1084, 1261
BsuRI	GGCC	9 cut(s)	30, 59, 107, 163, 210, 276, 740, 1086, 1286
BtgI	CCRYGG	3 cut(s)	25, 1535, 1584
BtgZI	GCGATG	1 cut(s)	664
BtsCI	GGATG	7 cut(s)	225, 755, 879, 1160, 1544, 1545, 1595
BtsI	GCAGTG	1 cut(s)	1064
BtsIMutI	CAGTG	2 cut(s)	339, 1064
Cac8I	GCNNGC	6 cut(s)	100, 154, 232, 274, 370, 1666
CfoI	GCGC	5 cut(s)	317, 377, 1093, 1216, 1308
Cfr10I	RCCGGY	2 cut(s)	248, 272
Cfr9I	CCCGGG	1 cut(s)	54
CseI	GACGC	9 cut(s)	187, 223, 382, 499, 842, 930, 977, 1270, 1510
CsiI	ACCWGGT	2 cut(s)	435, 1485
Csp6I	GTAC	3 cut(s)	624, 829, 964
CspAI	ACCGGT	1 cut(s)	248
CviQI	GTAC	3 cut(s)	624, 829, 964
DdeI	CTNAG	5 cut(s)	342, 750, 815, 1155, 1694
DinI	GGCGCC	1 cut(s)	376
DpnI	GATC	6 cut(s)	564, 569, 974, 1031, 1227, 1746
DpnII	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
DrdI	GACNNNNNNGTC	1 cut(s)	1695
DseDI	GACNNNNNNGTC	1 cut(s)	1695
EaeI	YGGCCR	2 cut(s)	738, 1284
EagI	CGGCCG	1 cut(s)	738
Eam1104I	CTCTTC	2 cut(s)	324, 849
EarI	CTCTTC	2 cut(s)	324, 849
EciI	GGCGGA	2 cut(s)	316, 866
Ecl136II	GAGCTC	2 cut(s)	152, 636
EclXI	CGGCCG	1 cut(s)	738
Eco105I	TACGTA	1 cut(s)	451
Eco130I	CCWWGG	3 cut(s)	25, 1535, 1584
Eco24I	GRGCYC	6 cut(s)	73, 154, 165, 500, 638, 902
Eco47I	GGWCC	3 cut(s)	1116, 1147, 1581
Eco52I	CGGCCG	1 cut(s)	738
Eco53kI	GAGCTC	2 cut(s)	152, 636
Eco88I	CYCGRG	1 cut(s)	54
EcoICRI	GAGCTC	2 cut(s)	152, 636
EcoNI	CCTNNNNNAGG	1 cut(s)	1603
EcoO109I	RGGNCCY	1 cut(s)	162
EcoRI	GAATTC	2 cut(s)	407, 1053
EcoRII	CCWGG	6 cut(s)	72, 435, 1086, 1118, 1411, 1485
EcoT14I	CCWWGG	3 cut(s)	25, 1535, 1584
EcoT38I	GRGCYC	6 cut(s)	73, 154, 165, 500, 638, 902
EgeI	GGCGCC	1 cut(s)	376
EheI	GGCGCC	1 cut(s)	376
ErhI	CCWWGG	3 cut(s)	25, 1535, 1584
Esp3I	CGTCTC	2 cut(s)	121, 544
FaqI	GGGAC	7 cut(s)	5, 497, 728, 1112, 1273, 1336, 1508
FauI	CCCGC	3 cut(s)	98, 209, 523
FblI	GTMKAC	1 cut(s)	354
Fnu4HI	GCNGC	9 cut(s)	373, 414, 465, 519, 939, 999, 1206, 1449, 1469
FokI	GGATG	7 cut(s)	232, 742, 866, 1147, 1531, 1552, 1582
FriOI	GRGCYC	6 cut(s)	73, 154, 165, 500, 638, 902
Fsp4HI	GCNGC	9 cut(s)	373, 414, 465, 519, 939, 999, 1206, 1449, 1469
FspBI	CTAG	2 cut(s)	1143, 1665
GlaI	GCGC	5 cut(s)	316, 376, 1092, 1215, 1307
GluI	GCNGC	9 cut(s)	373, 414, 465, 519, 939, 999, 1206, 1449, 1469
GsaI	CCCAGC	1 cut(s)	1085
HaeII	RGCGCY	2 cut(s)	378, 1309
HaeIII	GGCC	9 cut(s)	30, 59, 107, 163, 210, 276, 740, 1086, 1286
HgaI	GACGC	9 cut(s)	187, 223, 382, 499, 842, 930, 977, 1270, 1510
HhaI	GCGC	5 cut(s)	317, 377, 1093, 1216, 1308
Hin1I	GRCGYC	5 cut(s)	234, 375, 510, 941, 1521
Hin6I	GCGC	5 cut(s)	315, 375, 1091, 1214, 1306
HinP1I	GCGC	5 cut(s)	315, 375, 1091, 1214, 1306
HincII	GTYRAC	2 cut(s)	355, 1174
HindII	GTYRAC	2 cut(s)	355, 1174
HinfI	GANTC	7 cut(s)	309, 546, 558, 702, 865, 1019, 1724
Hpy166II	GTNNAC	6 cut(s)	355, 534, 811, 1150, 1174, 1688
Hpy188I	TCNGA	8 cut(s)	68, 149, 203, 567, 1007, 1034, 1112, 1230
Hpy188III	TCNNGA	6 cut(s)	543, 1198, 1258, 1561, 1623, 1733
Hpy8I	GTNNAC	6 cut(s)	355, 534, 811, 1150, 1174, 1688
Hpy99I	CGWCG	9 cut(s)	134, 203, 356, 473, 587, 590, 593, 836, 971
HpyAV	CCTTC	6 cut(s)	340, 409, 622, 1247, 1438, 1673
HpyCH4III	ACNGT	6 cut(s)	538, 623, 919, 1116, 1633, 1709
HpyCH4IV	ACGT	3 cut(s)	450, 1026, 1061
HpyCH4V	TGCA	6 cut(s)	172, 503, 667, 708, 1471, 1676
HpyF3I	CTNAG	5 cut(s)	342, 750, 815, 1155, 1694
HpySE526I	ACGT	3 cut(s)	450, 1026, 1061
Hsp92I	GRCGYC	5 cut(s)	234, 375, 510, 941, 1521
HspAI	GCGC	5 cut(s)	315, 375, 1091, 1214, 1306
KasI	GGCGCC	1 cut(s)	374
Kpn2I	TCCGGA	1 cut(s)	542
KroI	GCCGGC	1 cut(s)	272
KroNI	GCCGGC	1 cut(s)	274
Kzo9I	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
LguI	GCTCTTC	1 cut(s)	324
LmnI	GCTCC	6 cut(s)	161, 186, 428, 905, 960, 991
Lsp1109I	GCAGC	2 cut(s)	1455, 1460
LweI	GCATC	3 cut(s)	1521, 1530, 1663
MabI	ACCWGGT	2 cut(s)	435, 1485
MaeI	CTAG	2 cut(s)	1143, 1665
MaeII	ACGT	3 cut(s)	450, 1026, 1061
MaeIII	GTNAC	7 cut(s)	241, 394, 913, 1062, 1094, 1441, 1627
MalI	GATC	6 cut(s)	564, 569, 974, 1031, 1227, 1746
MboI	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
MboII	GAAGA	8 cut(s)	187, 311, 667, 866, 874, 1375, 1381, 1549
MfeI	CAATTG	2 cut(s)	1209, 1452
MflI	RGATCY	1 cut(s)	1225
MhlI	GDGCHC	9 cut(s)	16, 73, 154, 165, 500, 638, 902, 1361, 1638
MlsI	TGGCCA	1 cut(s)	1286
MluCI	AATT	6 cut(s)	407, 1053, 1209, 1452, 1473, 1712
MluNI	TGGCCA	1 cut(s)	1286
Mly113I	GGCGCC	1 cut(s)	375
MlyI	GAGTC	2 cut(s)	540, 1028
MmeI	TCCRAC	1 cut(s)	181
Mox20I	TGGCCA	1 cut(s)	1286
MroI	TCCGGA	1 cut(s)	542
MroNI	GCCGGC	1 cut(s)	272
MroXI	GAANNNNTTC	2 cut(s)	725, 1427
MscI	TGGCCA	1 cut(s)	1286
MseI	TTAA	4 cut(s)	422, 596, 873, 932
MslI	CAYNNNNRTG	1 cut(s)	1354
Msp20I	TGGCCA	1 cut(s)	1286
MspA1I	CMGCKG	1 cut(s)	186
MunI	CAATTG	2 cut(s)	1209, 1452
MvaI	CCWGG	6 cut(s)	74, 437, 1088, 1120, 1413, 1487
MvnI	CGCG	4 cut(s)	393, 413, 971, 1093
NaeI	GCCGGC	1 cut(s)	274
NarI	GGCGCC	1 cut(s)	375
NciI	CCSGG	9 cut(s)	55, 56, 104, 160, 278, 737, 746, 760, 980
NcoI	CCATGG	3 cut(s)	25, 1535, 1584
NdeII	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
NgoMIV	GCCGGC	1 cut(s)	272
NheI	GCTAGC	1 cut(s)	1664
NlaIV	GGNNCC	9 cut(s)	31, 163, 164, 376, 901, 987, 1148, 1491, 1582
NmeAIII	GCCGAG	1 cut(s)	192
NmuCI	GTSAC	5 cut(s)	241, 394, 913, 1062, 1441
NspI	RCATGY	3 cut(s)	172, 361, 1102
PciI	ACATGT	1 cut(s)	1098
PciSI	GCTCTTC	1 cut(s)	324
PcsI	WCGNNNNNNNCGW	5 cut(s)	231, 360, 567, 582, 972
PdiI	GCCGGC	1 cut(s)	274
PdmI	GAANNNNTTC	2 cut(s)	725, 1427
PfeI	GAWTC	5 cut(s)	309, 558, 702, 865, 1724
Pfl23II	CGTACG	1 cut(s)	963
PflFI	GACNNNGTC	1 cut(s)	197
PflMI	CCANNNNNTGG	1 cut(s)	1754
PinAI	ACCGGT	1 cut(s)	248
PkrI	GCNGC	9 cut(s)	374, 415, 466, 520, 940, 1000, 1207, 1450, 1470
Ple19I	CGATCG	1 cut(s)	975
PleI	GAGTC	2 cut(s)	540, 1027
PluTI	GGCGCC	1 cut(s)	378
PpsI	GAGTC	2 cut(s)	540, 1027
Ppu21I	YACGTR	1 cut(s)	451
PscI	ACATGT	1 cut(s)	1098
Psp124BI	GAGCTC	2 cut(s)	154, 638
Psp6I	CCWGG	6 cut(s)	72, 435, 1086, 1118, 1411, 1485
PspFI	CCCAGC	1 cut(s)	1081
PspGI	CCWGG	6 cut(s)	72, 435, 1086, 1118, 1411, 1485
PspLI	CGTACG	1 cut(s)	963
PspN4I	GGNNCC	9 cut(s)	31, 163, 164, 376, 901, 987, 1148, 1491, 1582
PspOMI	GGGCCC	1 cut(s)	161
PsuI	RGATCY	1 cut(s)	1225
PsyI	GACNNNGTC	1 cut(s)	197
PvuI	CGATCG	1 cut(s)	975
RsaI	GTAC	3 cut(s)	625, 830, 965
RsaNI	GTAC	3 cut(s)	624, 829, 964
RseI	CAYNNNNRTG	1 cut(s)	1354
SacI	GAGCTC	2 cut(s)	154, 638
SalI	GTCGAC	1 cut(s)	353
SapI	GCTCTTC	1 cut(s)	324
SaqAI	TTAA	4 cut(s)	422, 596, 873, 932
SatI	GCNGC	9 cut(s)	373, 414, 465, 519, 939, 999, 1206, 1449, 1469
Sau3AI	GATC	6 cut(s)	562, 567, 972, 1029, 1225, 1744
SchI	GAGTC	2 cut(s)	540, 1028
SduI	GDGCHC	9 cut(s)	16, 73, 154, 165, 500, 638, 902, 1361, 1638
SexAI	ACCWGGT	2 cut(s)	435, 1485
SfaNI	GCATC	3 cut(s)	1521, 1530, 1663
SfcI	CTRYAG	2 cut(s)	619, 1651
SfoI	GGCGCC	1 cut(s)	376
SinI	GGWCC	3 cut(s)	1116, 1147, 1581
SmaI	CCCGGG	1 cut(s)	56
SmiMI	CAYNNNNRTG	1 cut(s)	1354
SmlI	CTYRAG	2 cut(s)	46, 1731
SmoI	CTYRAG	2 cut(s)	46, 1731
SnaBI	TACGTA	1 cut(s)	451
Sse9I	AATT	6 cut(s)	407, 1053, 1209, 1452, 1473, 1712
SspDI	GGCGCC	1 cut(s)	374
SspI	AATATT	1 cut(s)	1514
SspMI	CTAG	2 cut(s)	1143, 1665
SstI	GAGCTC	2 cut(s)	154, 638
StyI	CCWWGG	3 cut(s)	25, 1535, 1584
TaaI	ACNGT	6 cut(s)	538, 623, 919, 1116, 1633, 1709
TaiI	ACGT	3 cut(s)	453, 1029, 1064
TaqI	TCGA	8 cut(s)	192, 354, 458, 561, 570, 950, 975, 1022
TasI	AATT	6 cut(s)	407, 1053, 1209, 1452, 1473, 1712
TauI	GCSGC	7 cut(s)	375, 416, 467, 521, 941, 1001, 1208
TfiI	GAWTC	5 cut(s)	309, 558, 702, 865, 1724
Tru1I	TTAA	4 cut(s)	422, 596, 873, 932
Tru9I	TTAA	4 cut(s)	422, 596, 873, 932
TscAI	CASTG	2 cut(s)	346, 1071
TseFI	GTSAC	5 cut(s)	241, 394, 913, 1062, 1441
TseI	GCWGC	2 cut(s)	1448, 1468
Tsp45I	GTSAC	5 cut(s)	241, 394, 913, 1062, 1441
TspDTI	ATGAA	4 cut(s)	667, 1381, 1420, 1649
TspGWI	ACGGA	5 cut(s)	103, 570, 892, 978, 1238
TspMI	CCCGGG	1 cut(s)	54
TspRI	CASTG	2 cut(s)	346, 1071
Tth111I	GACNNNGTC	1 cut(s)	197
Van91I	CCANNNNNTGG	1 cut(s)	1754
VpaK11BI	GGWCC	3 cut(s)	1116, 1147, 1581
XagI	CCTNNNNNAGG	1 cut(s)	1603
XapI	RAATTY	4 cut(s)	407, 1053, 1473, 1712
XceI	RCATGY	3 cut(s)	172, 361, 1102
XmaI	CCCGGG	1 cut(s)	54
XmiI	GTMKAC	1 cut(s)	354
XmnI	GAANNNNTTC	2 cut(s)	725, 1427
XspI	CTAG	2 cut(s)	1143, 1665

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.