MD03G1003700.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006325
Physical Location & Seq
Reverse (-)
274967 .. 277408
2442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1003700.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGAAAAGGAAGGGAAAACCTCTAAAGCCACAGAGTCGGACTTTTTGCTGCAATGGGGGAACAGAAAGAGGCCTAGATATTCAAAGTTGAATAAGGACGCAAGCTTGAGCAACAACAACAAATCGTCGGACTGTTTGAGGAAGAAGAAAATCACTTCTCGGGCCACCGATAAAGAGTCTCCGGCTCCTCATCCACTGCCCAATCGCATGGACAAGAGTTCAGCTTCGCCAATGAATAGTAGGAAGCTATTTGTTACTTCACCGGAGAAGGAAGATCGGTACTACACAACAAGGGGTTCATTAGGGTTGGACGAGAATGGGAAGGTGTTAGGATTAGATTATCCTGGTCCTGCAAGGGATGACAAAGGAAGCCATGCTTGGCCAAGGTTATTTCTATCATTGTCTAGTAAAGAAAAGGAAGAGGATTTCATGGCTATGAAAGGGTGTAAGCTACCTCAAAGGCCCAAGAAAAGAGCAAAATTGCTCCAGAAAAGCCTACTTTTGATTATGCCCGGTGTATGGTTATCGGATTTGTGCCAGGAGCGGTATGAAGTGAGGGAGAAGAAGACTTCAAAGAAGAGACCCAGAGGATTGAAGGCCATGGGAGGTATCGATGGCGAATCAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

210

Amino Acids

23.73

Weight (kDa)

9.86

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 134 - 183 1.3e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 544
AciI CCGC 1 cut(s) 544
AcoI YGGCCR 1 cut(s) 380
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 4 cut(s) 84, 91, 573, 595
AjnI CCWGG 2 cut(s) 343, 537
AjuI GAANNNNNNNTTGG 2 cut(s) 361, 393
AluBI AGCT 4 cut(s) 105, 224, 247, 451
AluI AGCT 4 cut(s) 105, 224, 247, 451
Alw26I GTCTC 2 cut(s) 183, 574
Ama87I CYCGRG 1 cut(s) 159
AoxI GGCC 5 cut(s) 71, 162, 380, 461, 597
ApeKI GCWGC 1 cut(s) 49
AspS9I GGNCC 3 cut(s) 162, 347, 462
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 1 cut(s) 252
AvaI CYCGRG 1 cut(s) 159
AvaII GGWCC 1 cut(s) 347
BalI TGGCCA 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 572
BbvI GCAGC 1 cut(s) 36
BccI CCATC 1 cut(s) 608
BciT130I CCWGG 2 cut(s) 345, 539
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 2 cut(s) 183, 574
BfaI CTAG 2 cut(s) 75, 405
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 345, 513, 539
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 162, 347, 462
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 3 cut(s) 345, 513, 539
BpiI GAAGAC 1 cut(s) 572
BpmI CTGGAG 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 127
BpuMI CCSGG 1 cut(s) 513
Bsa29I ATCGAT 1 cut(s) 612
BsaI GGTCTC 1 cut(s) 574
BsaJI CCNNGG 2 cut(s) 383, 600
BsaWI WCCGGW 1 cut(s) 262
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse3DI GCAATG 1 cut(s) 59
BseBI CCWGG 2 cut(s) 345, 539
BseCI ATCGAT 1 cut(s) 612
BseDI CCNNGG 2 cut(s) 383, 600
BseGI GGATG 2 cut(s) 190, 364
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMI GCAATG 1 cut(s) 59
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 1 cut(s) 36
BshFI GGCC 5 cut(s) 73, 164, 382, 463, 599
BshVI ATCGAT 1 cut(s) 612
BsiHKCI CYCGRG 1 cut(s) 159
BsiSI CCGG 3 cut(s) 182, 263, 513
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 2 cut(s) 183, 574
BsnI GGCC 5 cut(s) 73, 164, 382, 463, 599
Bso31I GGTCTC 1 cut(s) 574
BsoBI CYCGRG 1 cut(s) 159
Bsp143I GATC 1 cut(s) 274
Bsp19I CCATGG 1 cut(s) 600
BspACI CCGC 1 cut(s) 544
BspANI GGCC 5 cut(s) 73, 164, 382, 463, 599
BspDI ATCGAT 1 cut(s) 612
BspLI GGNNCC 1 cut(s) 186
BspTNI GGTCTC 1 cut(s) 574
BsrBI CCGCTC 1 cut(s) 544
BsrDI GCAATG 1 cut(s) 59
BssECI CCNNGG 2 cut(s) 383, 600
BssMI GATC 1 cut(s) 274
BssT1I CCWWGG 2 cut(s) 383, 600
Bst2UI CCWGG 2 cut(s) 345, 539
Bst4CI ACNGT 1 cut(s) 134
Bst6I CTCTTC 2 cut(s) 414, 572
BstC8I GCNNGC 1 cut(s) 103
BstDSI CCRYGG 1 cut(s) 600
BstF5I GGATG 2 cut(s) 190, 364
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 2 cut(s) 183, 574
BstMBI GATC 1 cut(s) 274
BstNI CCWGG 2 cut(s) 345, 539
BstSCI CCNGG 3 cut(s) 343, 511, 537
BstV1I GCAGC 1 cut(s) 36
BstV2I GAAGAC 1 cut(s) 572
BstXI CCANNNNNNTGG 1 cut(s) 208
Bsu15I ATCGAT 1 cut(s) 612
BsuRI GGCC 5 cut(s) 73, 164, 382, 463, 599
BsuTUI ATCGAT 1 cut(s) 612
BtgI CCRYGG 1 cut(s) 600
BtsCI GGATG 2 cut(s) 190, 364
BtsI GCAGTG 1 cut(s) 194
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 103
Cfr13I GGNCC 3 cut(s) 162, 347, 462
ClaI ATCGAT 1 cut(s) 612
CseI GACGC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 280
CviAII CATG 4 cut(s) 208, 374, 430, 601
CviQI GTAC 1 cut(s) 280
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 1 cut(s) 380
Eam1104I CTCTTC 2 cut(s) 414, 572
EarI CTCTTC 2 cut(s) 414, 572
Eco130I CCWWGG 2 cut(s) 383, 600
Eco147I AGGCCT 1 cut(s) 73
Eco31I GGTCTC 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 159
EcoRII CCWGG 2 cut(s) 343, 537
EcoT14I CCWWGG 2 cut(s) 383, 600
ErhI CCWWGG 2 cut(s) 383, 600
FaeI CATG 4 cut(s) 211, 377, 433, 604
FaiI YATR 8 cut(s) 209, 375, 431, 437, 509, 520, 549, 602
FalI AAGNNNNNCTT 2 cut(s) 361, 393
FatI CATG 4 cut(s) 207, 373, 429, 600
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 2 cut(s) 177, 371
Fsp4HI GCNGC 1 cut(s) 50
FspBI CTAG 2 cut(s) 75, 405
GluI GCNGC 1 cut(s) 50
GsuI CTGGAG 1 cut(s) 470
HaeIII GGCC 5 cut(s) 73, 164, 382, 463, 599
HapII CCGG 3 cut(s) 182, 263, 513
HgaI GACGC 1 cut(s) 107
Hin1II CATG 4 cut(s) 211, 377, 433, 604
HindIII AAGCTT 1 cut(s) 103
HinfI GANTC 3 cut(s) 35, 176, 620
HpaII CCGG 3 cut(s) 182, 263, 513
HphI GGTGA 1 cut(s) 252
Hpy188I TCNGA 4 cut(s) 40, 130, 529, 625
Hpy188III TCNNGA 1 cut(s) 487
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 5, 262, 316, 589
HpyCH4III ACNGT 1 cut(s) 134
HpyCH4V TGCA 2 cut(s) 52, 353
Hsp92II CATG 4 cut(s) 211, 377, 433, 604
Kzo9I GATC 1 cut(s) 274
LmnI GCTCC 3 cut(s) 190, 489, 541
Lsp1109I GCAGC 1 cut(s) 36
MaeI CTAG 2 cut(s) 75, 405
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 276
MbiI CCGCTC 1 cut(s) 544
MboI GATC 1 cut(s) 274
MboII GAAGA 7 cut(s) 154, 157, 284, 431, 574, 577, 589
MlsI TGGCCA 1 cut(s) 382
MluCI AATT 1 cut(s) 479
MluNI TGGCCA 1 cut(s) 382
MlyI GAGTC 2 cut(s) 44, 185
MmeI TCCRAC 3 cut(s) 18, 108, 288
MnlI CCTC 9 cut(s) 31, 63, 132, 198, 415, 465, 549, 581, 599
Mox20I TGGCCA 1 cut(s) 382
MscI TGGCCA 1 cut(s) 382
MslI CAYNNNNRTG 1 cut(s) 434
Msp20I TGGCCA 1 cut(s) 382
MspI CCGG 3 cut(s) 182, 263, 513
MspR9I CCNGG 3 cut(s) 345, 513, 539
MvaI CCWGG 2 cut(s) 345, 539
NciI CCSGG 1 cut(s) 513
NcoI CCATGG 1 cut(s) 600
NdeII GATC 1 cut(s) 274
NlaIII CATG 4 cut(s) 211, 377, 433, 604
NlaIV GGNNCC 1 cut(s) 186
PceI AGGCCT 1 cut(s) 73
PfeI GAWTC 1 cut(s) 620
PkrI GCNGC 1 cut(s) 51
PleI GAGTC 2 cut(s) 43, 184
PpsI GAGTC 2 cut(s) 43, 184
Psp6I CCWGG 2 cut(s) 343, 537
PspGI CCWGG 2 cut(s) 343, 537
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 3 cut(s) 162, 347, 462
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 434
SatI GCNGC 1 cut(s) 50
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 3 cut(s) 162, 347, 462
SchI GAGTC 2 cut(s) 44, 185
ScrFI CCNGG 3 cut(s) 345, 513, 539
SetI ASST 9 cut(s) 23, 107, 226, 249, 327, 389, 453, 457, 610
SinI GGWCC 1 cut(s) 347
SmiMI CAYNNNNRTG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 1 cut(s) 479
SseBI AGGCCT 1 cut(s) 73
SsiI CCGC 1 cut(s) 544
SspMI CTAG 2 cut(s) 75, 405
StuI AGGCCT 1 cut(s) 73
StyD4I CCNGG 3 cut(s) 343, 511, 537
StyI CCWWGG 2 cut(s) 383, 600
TaaI ACNGT 1 cut(s) 134
TaqI TCGA 1 cut(s) 612
TasI AATT 1 cut(s) 479
TfiI GAWTC 1 cut(s) 620
TscAI CASTG 1 cut(s) 201
TseI GCWGC 1 cut(s) 49
TspDTI ATGAA 5 cut(s) 248, 288, 418, 452, 564
TspRI CASTG 1 cut(s) 201
VpaK11BI GGWCC 1 cut(s) 347
XspI CTAG 2 cut(s) 75, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.