MD11G1003600.v1.1

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006325
Physical Location & Seq
Reverse (-)
298079 .. 300370
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1003600.v1.1.491

Sequence Viewer

Length: 741 bp
ATGAACTCTTCTCCTTCAAATATCTCACCTGCTGATTGTTTGGTCTGTTTGGGCTGTCTGAGACAAAGTGCATTTTTCACCGTTATCTCCATAGATCTTGAGATGGAAAAGGAAGGGAAAACCTCTAAGGCCACAGAGTCAGACTTTGTGCTGCAATGGGGGAACAGAAAGAGGCTTAGATGTTCAAAGTTGAAGCAGGACGAAAGCTTGAGCAACAACAACAAATCGTCGGACTGTTTGAGGAAGAAGAAAATCACTTCTCGGGCCGCCGATAAAGACTCTCCGGCTTCTCATCCACTGCCCAATCGCATGGACAAGAGTTCAACTTCGCCAATGAATAGTAGGAAGCTATTTGTTACTTCACCTGAGAAGGAAGATCGGTACTACACAACAAGGGGTTCATTAGGGTTGGACGAGAACGGGAAGGTGTTAGGATTAGATAATCCTGGTCCTGCAAGGGATGACAAAGGAAGCCGTGCTTGGCCAAGGTTATTTCTATCATTGTCTAGTAAAGAAAAGGAAGAGGATTTCATGGCTATGAAAGGGTGTAAGCTACCTCAAAGGCCCAAGAAAAGAGCAAAATTGCTCCAGAAAAGCCTACTTTTGGTGATGCCCGGTGTATGGTTATCGGATTTGTGCCAGGAGCGGTATGAAGTCAGGGAGAAGAAGACTTCAAAGAAGAGACCCAGAGGATTGAAGGCCATGGGAGGTATCGATAGCGAATCAGACAGAGAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

247

Amino Acids

27.7

Weight (kDa)

9.61

Isoelectric Point (pI)

51.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 168 - 217 1.8e-22 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 37
AccBSI CCGCTC 1 cut(s) 646
AciI CCGC 2 cut(s) 267, 646
AcoI YGGCCR 1 cut(s) 482
AfaI GTAC 1 cut(s) 383
AfiI CCNNNNNNNGG 2 cut(s) 604, 621
AgsI TTSAA 6 cut(s) 18, 186, 193, 324, 675, 697
AjiI CACGTC 1 cut(s) 738
AjnI CCWGG 2 cut(s) 445, 639
AjuI GAANNNNNNNTTGG 2 cut(s) 463, 495
AluBI AGCT 3 cut(s) 207, 349, 553
AluI AGCT 3 cut(s) 207, 349, 553
Alw26I GTCTC 3 cut(s) 55, 676, 728
Ama87I CYCGRG 1 cut(s) 261
AoxI GGCC 5 cut(s) 129, 264, 482, 563, 699
ApeKI GCWGC 1 cut(s) 151
AspS9I GGNCC 3 cut(s) 264, 449, 564
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 4 cut(s) 18, 70, 354, 619
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 1 cut(s) 449
BalI TGGCCA 1 cut(s) 484
BbsI GAAGAC 1 cut(s) 674
BbvI GCAGC 1 cut(s) 138
BccI CCATC 1 cut(s) 97
BceAI ACGGC 1 cut(s) 459
BciT130I CCWGG 2 cut(s) 447, 641
BcnI CCSGG 1 cut(s) 615
BcoDI GTCTC 3 cut(s) 55, 676, 728
BfaI CTAG 1 cut(s) 507
BfuAI ACCTGC 1 cut(s) 37
BglII AGATCT 1 cut(s) 94
BisI GCNGC 2 cut(s) 152, 267
BlsI GCNGC 2 cut(s) 153, 268
Bme1390I CCNGG 3 cut(s) 447, 615, 641
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 261
BmgBI CACGTC 1 cut(s) 738
BmgT120I GGNCC 3 cut(s) 264, 449, 564
BmrFI CCNGG 3 cut(s) 447, 615, 641
BmsI GCATC 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 674
BpmI CTGGAG 1 cut(s) 572
BpuEI CTTGAG 2 cut(s) 119, 229
BpuMI CCSGG 1 cut(s) 615
Bsa29I ATCGAT 1 cut(s) 714
BsaI GGTCTC 1 cut(s) 676
BsaJI CCNNGG 2 cut(s) 485, 702
Bsc4I CCNNNNNNNGG 2 cut(s) 604, 621
Bse3DI GCAATG 1 cut(s) 161
BseBI CCWGG 2 cut(s) 447, 641
BseCI ATCGAT 1 cut(s) 714
BseDI CCNNGG 2 cut(s) 485, 702
BseGI GGATG 2 cut(s) 292, 466
BseLI CCNNNNNNNGG 2 cut(s) 604, 621
BseMI GCAATG 1 cut(s) 161
BseMII CTCAG 2 cut(s) 50, 357
BseXI GCAGC 1 cut(s) 138
BshFI GGCC 5 cut(s) 131, 266, 484, 565, 701
BshVI ATCGAT 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 2 cut(s) 284, 615
BslI CCNNNNNNNGG 2 cut(s) 604, 621
BsmAI GTCTC 3 cut(s) 55, 676, 728
BsmBI CGTCTC 1 cut(s) 728
BsnI GGCC 5 cut(s) 131, 266, 484, 565, 701
Bso31I GGTCTC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 261
Bsp143I GATC 2 cut(s) 94, 376
Bsp19I CCATGG 1 cut(s) 702
BspACI CCGC 2 cut(s) 267, 646
BspANI GGCC 5 cut(s) 131, 266, 484, 565, 701
BspCNI CTCAG 2 cut(s) 51, 358
BspDI ATCGAT 1 cut(s) 714
BspMI ACCTGC 1 cut(s) 37
BspTNI GGTCTC 1 cut(s) 676
BsrBI CCGCTC 1 cut(s) 646
BsrDI GCAATG 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 485, 702
BssMI GATC 2 cut(s) 94, 376
BssT1I CCWWGG 2 cut(s) 485, 702
Bst2UI CCWGG 2 cut(s) 447, 641
Bst4CI ACNGT 2 cut(s) 82, 236
Bst6I CTCTTC 3 cut(s) 13, 516, 674
BstDEI CTNAG 4 cut(s) 59, 126, 176, 366
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 2 cut(s) 292, 466
BstKTI GATC 2 cut(s) 97, 379
BstMAI GTCTC 3 cut(s) 55, 676, 728
BstMBI GATC 2 cut(s) 94, 376
BstNI CCWGG 2 cut(s) 447, 641
BstSCI CCNGG 3 cut(s) 445, 613, 639
BstV1I GCAGC 1 cut(s) 138
BstV2I GAAGAC 1 cut(s) 674
BstX2I RGATCY 1 cut(s) 94
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 714
BsuRI GGCC 5 cut(s) 131, 266, 484, 565, 701
BsuTUI ATCGAT 1 cut(s) 714
BtgI CCRYGG 1 cut(s) 702
BtrI CACGTC 1 cut(s) 738
BtsCI GGATG 2 cut(s) 292, 466
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 296
BveI ACCTGC 1 cut(s) 37
Cfr13I GGNCC 3 cut(s) 264, 449, 564
ClaI ATCGAT 1 cut(s) 714
Csp6I GTAC 1 cut(s) 382
CviAII CATG 3 cut(s) 310, 532, 703
CviQI GTAC 1 cut(s) 382
DdeI CTNAG 4 cut(s) 59, 126, 176, 366
DpnI GATC 2 cut(s) 96, 378
DpnII GATC 2 cut(s) 94, 376
EaeI YGGCCR 1 cut(s) 482
Eam1104I CTCTTC 3 cut(s) 13, 516, 674
EarI CTCTTC 3 cut(s) 13, 516, 674
Eco130I CCWWGG 2 cut(s) 485, 702
Eco31I GGTCTC 1 cut(s) 676
Eco47I GGWCC 1 cut(s) 449
Eco88I CYCGRG 1 cut(s) 261
EcoRII CCWGG 2 cut(s) 445, 639
EcoT14I CCWWGG 2 cut(s) 485, 702
ErhI CCWWGG 2 cut(s) 485, 702
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 3 cut(s) 313, 535, 706
FaiI YATR 7 cut(s) 92, 311, 533, 539, 622, 651, 704
FalI AAGNNNNNCTT 2 cut(s) 463, 495
FatI CATG 3 cut(s) 309, 531, 702
Fnu4HI GCNGC 2 cut(s) 152, 267
FokI GGATG 2 cut(s) 279, 473
Fsp4HI GCNGC 2 cut(s) 152, 267
FspBI CTAG 1 cut(s) 507
GluI GCNGC 2 cut(s) 152, 267
GsuI CTGGAG 1 cut(s) 572
HaeIII GGCC 5 cut(s) 131, 266, 484, 565, 701
HapII CCGG 2 cut(s) 284, 615
Hin1II CATG 3 cut(s) 313, 535, 706
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 3 cut(s) 137, 278, 722
HpaII CCGG 2 cut(s) 284, 615
HphI GGTGA 4 cut(s) 18, 70, 354, 619
Hpy188I TCNGA 5 cut(s) 60, 142, 232, 631, 727
Hpy188III TCNNGA 2 cut(s) 98, 589
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 5 cut(s) 24, 107, 364, 418, 691
HpyCH4III ACNGT 2 cut(s) 82, 236
HpyCH4IV ACGT 1 cut(s) 737
HpyCH4V TGCA 3 cut(s) 71, 154, 455
HpyF3I CTNAG 4 cut(s) 59, 126, 176, 366
HpySE526I ACGT 1 cut(s) 737
Hsp92II CATG 3 cut(s) 313, 535, 706
Kzo9I GATC 2 cut(s) 94, 376
LmnI GCTCC 2 cut(s) 591, 643
Lsp1109I GCAGC 1 cut(s) 138
LweI GCATC 1 cut(s) 600
MaeI CTAG 1 cut(s) 507
MaeII ACGT 1 cut(s) 737
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 96, 378
MbiI CCGCTC 1 cut(s) 646
MboI GATC 2 cut(s) 94, 376
MboII GAAGA 7 cut(s) 256, 259, 386, 533, 676, 679, 691
MflI RGATCY 1 cut(s) 94
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 1 cut(s) 581
MluNI TGGCCA 1 cut(s) 484
MlyI GAGTC 2 cut(s) 146, 272
MmeI TCCRAC 2 cut(s) 210, 390
MnlI CCTC 7 cut(s) 133, 165, 234, 517, 567, 683, 701
Mox20I TGGCCA 1 cut(s) 484
MscI TGGCCA 1 cut(s) 484
MslI CAYNNNNRTG 1 cut(s) 536
Msp20I TGGCCA 1 cut(s) 484
MspI CCGG 2 cut(s) 284, 615
MspR9I CCNGG 3 cut(s) 447, 615, 641
MvaI CCWGG 2 cut(s) 447, 641
NciI CCSGG 1 cut(s) 615
NcoI CCATGG 1 cut(s) 702
NdeII GATC 2 cut(s) 94, 376
NlaIII CATG 3 cut(s) 313, 535, 706
PaqCI CACCTGC 1 cut(s) 37
PfeI GAWTC 1 cut(s) 722
PkrI GCNGC 2 cut(s) 153, 268
PleI GAGTC 2 cut(s) 145, 272
PpsI GAGTC 2 cut(s) 145, 272
Psp6I CCWGG 2 cut(s) 445, 639
PspGI CCWGG 2 cut(s) 445, 639
PspPI GGNCC 3 cut(s) 264, 449, 564
PsuI RGATCY 1 cut(s) 94
RsaI GTAC 1 cut(s) 383
RsaNI GTAC 1 cut(s) 382
RseI CAYNNNNRTG 1 cut(s) 536
SatI GCNGC 2 cut(s) 152, 267
Sau3AI GATC 2 cut(s) 94, 376
Sau96I GGNCC 3 cut(s) 264, 449, 564
SchI GAGTC 2 cut(s) 146, 272
ScrFI CCNGG 3 cut(s) 447, 615, 641
SfaNI GCATC 1 cut(s) 600
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 98, 208
SmoI CTYRAG 2 cut(s) 98, 208
Sse9I AATT 1 cut(s) 581
SsiI CCGC 2 cut(s) 267, 646
SspMI CTAG 1 cut(s) 507
StyD4I CCNGG 3 cut(s) 445, 613, 639
StyI CCWWGG 2 cut(s) 485, 702
TaaI ACNGT 2 cut(s) 82, 236
TaiI ACGT 1 cut(s) 740
TaqI TCGA 1 cut(s) 714
TasI AATT 1 cut(s) 581
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 1 cut(s) 722
TscAI CASTG 1 cut(s) 303
TseI GCWGC 1 cut(s) 151
TspDTI ATGAA 6 cut(s) 17, 350, 390, 520, 554, 666
TspRI CASTG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 449
XspI CTAG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.