MD03G1029500.v1.1

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001306
Physical Location & Seq
Reverse (-)
2406895 .. 2410716
3822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1029500.v1.1.491

Sequence Viewer

Length: 1833 bp
ATGTCGATGGCCTCTAAATCCGGTGCCAGAAGTTCTTCCCCGGAAACTCAGAGGAAATTAATTGGTAGTCAAACAACAGAAGGAAATTTTCGCAGACCTTCACCTCTACCAATTACAAAGATGAGCAAATCGGAGCCAGTCACTCCCAAAAAAATTCCTAGGCATGTTCAACAAGATGCACTGAATCAGGTTTCCACAGAAATCACTGAAGAAAAGAACTATAAGATTCCTCATCAGAAGGAGTCTGCCAAAACTTTAGCGGATCAGTTAGGTTCAGCTTTATCACTGGGTGATGCAAAACAGGTTCCACATGTGGATCCTGTAGTGAGTGGGACTAGGAATTTTCCAGAAGCCGGTGATCAAAAAAAGAAAACATCAGAAAATGGAGTTAGCCCAGCTTCAGCGAAAGTAAGTGATGATGGGACCAGCAGTATTGCCAAGACTAGTGGAAGCGCCAAGTTGAGTGGTCGAGCTGATTTTGTTGAGAGTGGGAAGAGCAGTATTTGTAGAGGCAGCACAAGCAGCGACATAAGTGACGAAAGTACTTGTAGCAGCTTTAGTAGCGCTATCAACAAACCCCACAAGGCAAATGACATACATTGGGAAGCCATACAGGCTGTCCGTTCAAAGGATGGTGTATTTGGTTTGGGCCATTTCAGACTTCTGAAGAAGTTGGGCTGTGGAGATATTGGAAGTGTCTATCTTTCAGAGTTGAGCGGAACAAAATGTTATTTTGCAATGAAAGTTATGGACAAAGCATCTTTGGCTAACCGCAAAAAACTTCTTCGTGCTCAGACAGAGAGAGAAATACTTCAATGTTTAGACCATCCTTTCCTTCCAACCCTATATACCCATTTCGAGACAGAGAAGTATTCATGTCTGGTGATGGAGTTCTGCCCTGGAGGTGACTTGCACACACTTAGGCAGAGACAACCCGGAAAGCATTTCACTGAGCAAGCAGTGAAGTTCTATGTAGCTGAGGTTCTCCTTGCGCTTGAATATCTTCACATGCTTGGGATTGTTTATCGTGACCTCAAGCCAGAAAATGTCCTTGTGAGAGATGATGGGCACATAATGCTTTCTGACTTTGACCTTTCCCTGCGCTGCATTGTCAGCCCAACCCTTGTCAAATCTTCAGCCCCCGATTCTGAACCTTTTCGAAGGAACTCAGCTTATTGTGTGCAACCTGCTTGCATCGAGCCCTCTTGTATTCAGCCATCTTGTGTGGTGCCTACAACATGTTTCTCCCCTCGTTTCTTTTCAAGTAAATCCAAGAAAGACCGTAAACCCAAGAATGAAGTAGGAAACCAAGTACGGCCATTGCCAGAACTCATGGCTGAGCCAACCAATGCTCGCTCGATGTCATTTGTTGGGACCCATGAGTATTTGGCACCAGAAATTATTAAAGGTGAAGGGCACGGCAGTGCTGTTGATTGGTGGACCTTCGGGATCTTTCTGTATGAGCTGTTGTTTGGTAAAACACCTTTCAAGGGATCAGGGAATCGAGCCACCCTGTTCAACGTGGTAGGTCAGCCATTGAGATTCCCGGAAACACCAGTGGTTAGCTTTTCAGCAAGGGATCTGATCAGGGGCCTACTGGTGAAAGAACCACAGCATAGGCTGGCATATAAGCGAGGAGCAACGGAGATTAAACAGCATCCGTTCTTTGAGGGTGTTAATTGGGCGTTGATTCGATGTGCAAGTCCTCCAGATATTCCGAAACCAGTCGAGTTTGAGCGGATTTCAGCCCCCGCAGTGTCAACCGGTGATAAGGCTACTGCTGCTGCTGCTCATCCTGATCAAAACAATTATCTGGAATTTGATTTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

611

Amino Acids

67.24

Weight (kDa)

8.87

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 220 - 368 8.3e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 220 - 368 1.1e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 453 - 556 9.9e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 460 - 543 5.5e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1195
AccB1I GGYRCC 3 cut(s) 23, 1228, 1390
AccBSI CCGCTC 2 cut(s) 717, 1738
AciI CCGC 5 cut(s) 260, 717, 772, 1738, 1752
AclWI GGATC 6 cut(s) 270, 311, 324, 1457, 1501, 1587
AcoI YGGCCR 1 cut(s) 1316
AcsI RAATTY 4 cut(s) 85, 153, 340, 1817
AcuI CTGAAG 4 cut(s) 228, 384, 686, 1119
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 2 cut(s) 544, 1314
AfeI AGCGCT 1 cut(s) 565
AfiI CCNNNNNNNGG 3 cut(s) 353, 628, 1490
AflIII ACRYGT 2 cut(s) 310, 1238
AgeI ACCGGT 1 cut(s) 1763
AgsI TTSAA 7 cut(s) 170, 627, 815, 998, 1263, 1489, 1519
AhlI ACTAGT 1 cut(s) 443
AjnI CCWGG 1 cut(s) 898
AleI CACNNNNGTG 1 cut(s) 1422
AluBI AGCT 8 cut(s) 278, 398, 473, 555, 977, 1172, 1465, 1566
AluI AGCT 8 cut(s) 278, 398, 473, 555, 977, 1172, 1465, 1566
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 2 cut(s) 854, 922
AlwI GGATC 6 cut(s) 270, 311, 324, 1457, 1501, 1587
Aor51HI AGCGCT 1 cut(s) 565
AoxI GGCC 4 cut(s) 9, 649, 1316, 1591
ApeKI GCWGC 7 cut(s) 513, 522, 552, 1104, 1781, 1784, 1787
ApoI RAATTY 4 cut(s) 85, 153, 340, 1817
AseI ATTAAT 1 cut(s) 59
AsiGI ACCGGT 1 cut(s) 1763
Asp700I GAANNNNTTC 4 cut(s) 34, 810, 1002, 1155
AspA2I CCTAGG 1 cut(s) 158
AspLEI GCGC 4 cut(s) 455, 566, 994, 1104
AspS9I GGNCC 5 cut(s) 423, 649, 1374, 1440, 1591
AsuC2I CCSGG 3 cut(s) 41, 936, 1547
AsuHPI GGTGA 8 cut(s) 93, 302, 368, 895, 917, 1421, 1612, 1778
AsuII TTCGAA 1 cut(s) 1159
AvaII GGWCC 3 cut(s) 423, 1374, 1440
AvrII CCTAGG 1 cut(s) 158
BaeGI GKGCMC 2 cut(s) 1071, 1419
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 3 cut(s) 23, 1228, 1390
BanII GRGCYC 1 cut(s) 1203
Bbv12I GWGCWC 1 cut(s) 793
BbvCI CCTCAGC 1 cut(s) 978
BbvI GCAGC 7 cut(s) 525, 534, 564, 1091, 1768, 1771, 1774
BccI CCATC 6 cut(s) 413, 626, 834, 880, 1058, 1225
BceAI ACGGC 2 cut(s) 1331, 1435
BciT130I CCWGG 1 cut(s) 900
BclI TGATCA 3 cut(s) 358, 1584, 1798
BcnI CCSGG 3 cut(s) 41, 936, 1547
BcoDI GTCTC 2 cut(s) 854, 922
BcuI ACTAGT 1 cut(s) 443
BfaI CTAG 3 cut(s) 159, 336, 444
BfmI CTRYAG 1 cut(s) 321
BfoI RGCGCY 2 cut(s) 456, 567
BfuAI ACCTGC 1 cut(s) 1195
BglI GCCNNNNNGGC 1 cut(s) 614
BisI GCNGC 7 cut(s) 514, 523, 553, 1105, 1782, 1785, 1788
BlnI CCTAGG 1 cut(s) 158
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 7 cut(s) 515, 524, 554, 1106, 1783, 1786, 1789
BmcAI AGTACT 1 cut(s) 544
Bme1390I CCNGG 4 cut(s) 41, 900, 936, 1547
Bme18I GGWCC 3 cut(s) 423, 1374, 1440
BmgT120I GGNCC 5 cut(s) 423, 649, 1374, 1440, 1591
BmrFI CCNGG 4 cut(s) 41, 900, 936, 1547
BmrI ACTGGG 1 cut(s) 296
BmsI GCATC 5 cut(s) 166, 283, 767, 1203, 1666
BmuI ACTGGG 1 cut(s) 296
BpmI CTGGAG 2 cut(s) 921, 1692
Bpu10I CCTNAGC 1 cut(s) 978
Bpu1102I GCTNAGC 1 cut(s) 1338
Bpu14I TTCGAA 1 cut(s) 1159
BpuEI CTTGAG 1 cut(s) 1019
BpuMI CCSGG 3 cut(s) 41, 936, 1547
BsaJI CCNNGG 3 cut(s) 39, 158, 898
BsaWI WCCGGW 2 cut(s) 20, 1763
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 628, 1490
Bse118I RCCGGY 2 cut(s) 353, 1763
Bse1I ACTGG 5 cut(s) 137, 291, 1556, 1602, 1724
Bse3DI GCAATG 2 cut(s) 744, 1319
BseBI CCWGG 1 cut(s) 900
BseDI CCNNGG 3 cut(s) 39, 158, 898
BseGI GGATG 4 cut(s) 637, 826, 1657, 1792
BseLI CCNNNNNNNGG 3 cut(s) 353, 628, 1490
BseMI GCAATG 2 cut(s) 744, 1319
BseMII CTCAG 6 cut(s) 62, 806, 942, 969, 1182, 1329
BseNI ACTGG 5 cut(s) 137, 291, 1556, 1602, 1724
BseRI GAGGAG 1 cut(s) 1650
BseSI GKGCMC 2 cut(s) 1071, 1419
BseXI GCAGC 7 cut(s) 525, 534, 564, 1091, 1768, 1771, 1774
BseYI CCCAGC 1 cut(s) 394
BshFI GGCC 4 cut(s) 11, 651, 1318, 1593
BshNI GGYRCC 3 cut(s) 23, 1228, 1390
BshTI ACCGGT 1 cut(s) 1763
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 6 cut(s) 21, 41, 354, 936, 1547, 1764
BslFI GGGAC 3 cut(s) 346, 436, 1387
BslI CCNNNNNNNGG 3 cut(s) 353, 628, 1490
BsmAI GTCTC 2 cut(s) 854, 922
BsmFI GGGAC 3 cut(s) 346, 436, 1387
BsnI GGCC 4 cut(s) 11, 651, 1318, 1593
Bsp119I TTCGAA 1 cut(s) 1159
Bsp1286I GDGCHC 4 cut(s) 793, 1071, 1203, 1419
Bsp143I GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
Bsp1720I GCTNAGC 1 cut(s) 1338
BspACI CCGC 5 cut(s) 260, 717, 772, 1738, 1752
BspANI GGCC 4 cut(s) 11, 651, 1318, 1593
BspCNI CTCAG 6 cut(s) 61, 805, 943, 970, 1181, 1330
BspMI ACCTGC 1 cut(s) 1195
BspPI GGATC 6 cut(s) 270, 311, 324, 1457, 1501, 1587
BspQI GCTCTTC 1 cut(s) 488
BspT104I TTCGAA 1 cut(s) 1159
BspT107I GGYRCC 3 cut(s) 23, 1228, 1390
BsrBI CCGCTC 2 cut(s) 717, 1738
BsrDI GCAATG 2 cut(s) 744, 1319
BsrFI RCCGGY 2 cut(s) 353, 1763
BsrI ACTGG 5 cut(s) 137, 291, 1556, 1602, 1724
BssAI RCCGGY 2 cut(s) 353, 1763
BssECI CCNNGG 3 cut(s) 39, 158, 898
BssMI GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 1 cut(s) 900
Bst4CI ACNGT 1 cut(s) 1283
Bst6I CTCTTC 1 cut(s) 488
BstAPI GCANNNNNTGC 1 cut(s) 1075
BstBI TTCGAA 1 cut(s) 1159
BstC8I GCNNGC 4 cut(s) 957, 1192, 1354, 1623
BstDEI CTNAG 7 cut(s) 48, 792, 920, 951, 978, 1168, 1338
BstF5I GGATG 4 cut(s) 637, 826, 1657, 1792
BstH2I RGCGCY 2 cut(s) 456, 567
BstHHI GCGC 4 cut(s) 455, 566, 994, 1104
BstKTI GATC 8 cut(s) 265, 319, 361, 1452, 1496, 1582, 1587, 1801
BstMAI GTCTC 2 cut(s) 854, 922
BstMBI GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
BstMWI GCNNNNNNNGC 9 cut(s) 519, 522, 561, 614, 764, 1075, 1113, 1781, 1787
BstNI CCWGG 1 cut(s) 900
BstNSI RCATGY 4 cut(s) 167, 314, 1012, 1242
BstSCI CCNGG 4 cut(s) 39, 898, 934, 1545
BstSFI CTRYAG 1 cut(s) 321
BstSLI GKGCMC 2 cut(s) 1071, 1419
BstV1I GCAGC 7 cut(s) 525, 534, 564, 1091, 1768, 1771, 1774
BstX2I RGATCY 3 cut(s) 316, 1449, 1579
BstYI RGATCY 3 cut(s) 316, 1449, 1579
BsuRI GGCC 4 cut(s) 11, 651, 1318, 1593
BtsCI GGATG 4 cut(s) 637, 826, 1657, 1792
BtsI GCAGTG 3 cut(s) 966, 1429, 1761
BtsIMutI CAGTG 8 cut(s) 179, 204, 284, 948, 966, 1429, 1563, 1761
BveI ACCTGC 1 cut(s) 1195
Cac8I GCNNGC 4 cut(s) 957, 1192, 1354, 1623
CfoI GCGC 4 cut(s) 455, 566, 994, 1104
Cfr10I RCCGGY 2 cut(s) 353, 1763
Cfr13I GGNCC 5 cut(s) 423, 649, 1374, 1440, 1591
Csp6I GTAC 2 cut(s) 543, 1313
CspAI ACCGGT 1 cut(s) 1763
CspCI CAANNNNNGTGG 4 cut(s) 427, 445, 462, 480
CviAII CATG 7 cut(s) 164, 311, 876, 1009, 1239, 1333, 1379
CviQI GTAC 2 cut(s) 543, 1313
DdeI CTNAG 7 cut(s) 48, 792, 920, 951, 978, 1168, 1338
DpnI GATC 8 cut(s) 264, 318, 360, 1451, 1495, 1581, 1586, 1800
DpnII GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
DraIII CACNNNGTG 1 cut(s) 290
EaeI YGGCCR 1 cut(s) 1316
Eam1104I CTCTTC 1 cut(s) 488
EarI CTCTTC 1 cut(s) 488
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 1203
Eco47I GGWCC 3 cut(s) 423, 1374, 1440
Eco47III AGCGCT 1 cut(s) 565
Eco57I CTGAAG 4 cut(s) 228, 384, 686, 1119
EcoO109I RGGNCCY 2 cut(s) 1374, 1591
EcoRII CCWGG 1 cut(s) 898
EcoT14I CCWWGG 1 cut(s) 158
EcoT38I GRGCYC 1 cut(s) 1203
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 7 cut(s) 167, 314, 879, 1012, 1242, 1336, 1382
FaqI GGGAC 3 cut(s) 346, 436, 1387
FatI CATG 7 cut(s) 163, 310, 875, 1008, 1238, 1332, 1378
FauI CCCGC 1 cut(s) 1759
FbaI TGATCA 3 cut(s) 358, 1584, 1798
Fnu4HI GCNGC 7 cut(s) 514, 523, 553, 1105, 1782, 1785, 1788
FokI GGATG 4 cut(s) 644, 813, 1644, 1779
FriOI GRGCYC 1 cut(s) 1203
Fsp4HI GCNGC 7 cut(s) 514, 523, 553, 1105, 1782, 1785, 1788
FspBI CTAG 3 cut(s) 159, 336, 444
GlaI GCGC 4 cut(s) 454, 565, 993, 1103
GluI GCNGC 7 cut(s) 514, 523, 553, 1105, 1782, 1785, 1788
GsaI CCCAGC 1 cut(s) 398
GsuI CTGGAG 2 cut(s) 921, 1692
HaeII RGCGCY 2 cut(s) 456, 567
HaeIII GGCC 4 cut(s) 11, 651, 1318, 1593
HapII CCGG 6 cut(s) 21, 41, 354, 936, 1547, 1764
HhaI GCGC 4 cut(s) 455, 566, 994, 1104
Hin1II CATG 7 cut(s) 167, 314, 879, 1012, 1242, 1336, 1382
Hin6I GCGC 4 cut(s) 453, 564, 992, 1102
HinP1I GCGC 4 cut(s) 453, 564, 992, 1102
HincII GTYRAC 1 cut(s) 1761
HindII GTYRAC 1 cut(s) 1761
HinfI GANTC 7 cut(s) 184, 226, 242, 1145, 1501, 1542, 1690
HpaII CCGG 6 cut(s) 21, 41, 354, 936, 1547, 1764
HphI GGTGA 8 cut(s) 93, 302, 368, 895, 917, 1421, 1612, 1778
Hpy166II GTNNAC 3 cut(s) 1286, 1440, 1761
Hpy188III TCNNGA 7 cut(s) 347, 859, 1028, 1447, 1709, 1796, 1814
Hpy8I GTNNAC 3 cut(s) 1286, 1440, 1761
HpyAV CCTTC 7 cut(s) 74, 108, 232, 845, 1155, 1406, 1453
HpyCH4III ACNGT 1 cut(s) 1283
HpyCH4IV ACGT 1 cut(s) 1521
HpyCH4V TGCA 8 cut(s) 179, 296, 737, 913, 1107, 1183, 1194, 1700
HpyF10VI GCNNNNNNNGC 9 cut(s) 519, 522, 561, 614, 764, 1075, 1113, 1781, 1787
HpyF3I CTNAG 7 cut(s) 48, 792, 920, 951, 978, 1168, 1338
HpySE526I ACGT 1 cut(s) 1521
Hsp92II CATG 7 cut(s) 167, 314, 879, 1012, 1242, 1336, 1382
HspAI GCGC 4 cut(s) 453, 564, 992, 1102
KflI GGGWCCC 1 cut(s) 1374
Ksp22I TGATCA 3 cut(s) 358, 1584, 1798
Kzo9I GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 2 cut(s) 133, 1637
Lsp1109I GCAGC 7 cut(s) 525, 534, 564, 1091, 1768, 1771, 1774
LweI GCATC 5 cut(s) 166, 283, 767, 1203, 1666
MaeI CTAG 3 cut(s) 159, 336, 444
MaeII ACGT 1 cut(s) 1521
MaeIII GTNAC 4 cut(s) 139, 533, 905, 1028
MalI GATC 8 cut(s) 264, 318, 360, 1451, 1495, 1581, 1586, 1800
MbiI CCGCTC 2 cut(s) 717, 1738
MboI GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
MboII GAAGA 7 cut(s) 27, 221, 505, 679, 776, 995, 1125
MflI RGATCY 3 cut(s) 316, 1449, 1579
MhlI GDGCHC 4 cut(s) 793, 1071, 1203, 1419
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 863
MroXI GAANNNNTTC 4 cut(s) 34, 810, 1002, 1155
MseI TTAA 5 cut(s) 59, 1404, 1650, 1677, 1831
MslI CAYNNNNRTG 1 cut(s) 1422
MspI CCGG 6 cut(s) 21, 41, 354, 936, 1547, 1764
MspR9I CCNGG 4 cut(s) 41, 900, 936, 1547
MvaI CCWGG 1 cut(s) 900
MwoI GCNNNNNNNGC 9 cut(s) 519, 522, 561, 614, 764, 1075, 1113, 1781, 1787
NciI CCSGG 3 cut(s) 41, 936, 1547
NdeII GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
NlaIII CATG 7 cut(s) 167, 314, 879, 1012, 1242, 1336, 1382
NmuCI GTSAC 4 cut(s) 139, 533, 905, 1028
NspI RCATGY 4 cut(s) 167, 314, 1012, 1242
NspV TTCGAA 1 cut(s) 1159
OliI CACNNNNGTG 1 cut(s) 1422
PciI ACATGT 2 cut(s) 310, 1238
PciSI GCTCTTC 1 cut(s) 488
PdmI GAANNNNTTC 4 cut(s) 34, 810, 1002, 1155
PfeI GAWTC 6 cut(s) 184, 226, 1145, 1501, 1542, 1690
PfoI TCCNGGA 1 cut(s) 1545
PinAI ACCGGT 1 cut(s) 1763
PkrI GCNGC 7 cut(s) 515, 524, 554, 1106, 1783, 1786, 1789
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PpuMI RGGWCCY 1 cut(s) 1374
PscI ACATGT 2 cut(s) 310, 1238
PshBI ATTAAT 1 cut(s) 59
Psp5II RGGWCCY 1 cut(s) 1374
Psp6I CCWGG 1 cut(s) 898
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 1 cut(s) 898
PspPI GGNCC 5 cut(s) 423, 649, 1374, 1440, 1591
PspPPI RGGWCCY 1 cut(s) 1374
PsrI GAACNNNNNNTAC 2 cut(s) 966, 998
PsuI RGATCY 3 cut(s) 316, 1449, 1579
RsaI GTAC 2 cut(s) 544, 1314
RsaNI GTAC 2 cut(s) 543, 1313
RseI CAYNNNNRTG 1 cut(s) 1422
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 5 cut(s) 59, 1404, 1650, 1677, 1831
SatI GCNGC 7 cut(s) 514, 523, 553, 1105, 1782, 1785, 1788
Sau3AI GATC 8 cut(s) 262, 316, 358, 1449, 1493, 1579, 1584, 1798
Sau96I GGNCC 5 cut(s) 423, 649, 1374, 1440, 1591
ScaI AGTACT 1 cut(s) 544
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 4 cut(s) 41, 900, 936, 1547
SduI GDGCHC 4 cut(s) 793, 1071, 1203, 1419
SfaNI GCATC 5 cut(s) 166, 283, 767, 1203, 1666
SfcI CTRYAG 1 cut(s) 321
SfuI TTCGAA 1 cut(s) 1159
SinI GGWCC 3 cut(s) 423, 1374, 1440
SmiMI CAYNNNNRTG 1 cut(s) 1422
SmlI CTYRAG 1 cut(s) 1034
SmoI CTYRAG 1 cut(s) 1034
SpeI ACTAGT 1 cut(s) 443
SsiI CCGC 5 cut(s) 260, 717, 772, 1738, 1752
SspMI CTAG 3 cut(s) 159, 336, 444
StyD4I CCNGG 4 cut(s) 39, 898, 934, 1545
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 1 cut(s) 1283
TaiI ACGT 1 cut(s) 1524
TaqI TCGA 9 cut(s) 5, 469, 858, 1159, 1197, 1358, 1504, 1693, 1728
TatI WGTACW 1 cut(s) 542
TfiI GAWTC 6 cut(s) 184, 226, 1145, 1501, 1542, 1690
Tru1I TTAA 5 cut(s) 59, 1404, 1650, 1677, 1831
Tru9I TTAA 5 cut(s) 59, 1404, 1650, 1677, 1831
TscAI CASTG 8 cut(s) 186, 211, 291, 955, 966, 1429, 1563, 1761
TseFI GTSAC 4 cut(s) 139, 533, 905, 1028
TseI GCWGC 7 cut(s) 513, 522, 552, 1104, 1781, 1784, 1787
Tsp45I GTSAC 4 cut(s) 139, 533, 905, 1028
TspDTI ATGAA 3 cut(s) 755, 864, 1311
TspGWI ACGGA 3 cut(s) 611, 1650, 1658
TspRI CASTG 8 cut(s) 186, 211, 291, 955, 966, 1429, 1563, 1761
VpaK11BI GGWCC 3 cut(s) 423, 1374, 1440
VspI ATTAAT 1 cut(s) 59
XapI RAATTY 4 cut(s) 85, 153, 340, 1817
XceI RCATGY 4 cut(s) 167, 314, 1012, 1242
XmaJI CCTAGG 1 cut(s) 158
XmnI GAANNNNTTC 4 cut(s) 34, 810, 1002, 1155
XspI CTAG 3 cut(s) 159, 336, 444
ZrmI AGTACT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.