Rorug05G0448000

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001306
Physical Location & Seq
Forward (+)
62013105 .. 62014730
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0448000.1

Sequence Viewer

Length: 1338 bp
ATGAAGTATGCCTTCAGAATGAACACACCGTCAAGAGAGTATGATCATCTGTCAAGGCGTGCCATAAGCTATGCTCAAGGTCTGCCTTTAGCACTTAAAGTCTTGGGAGCTTCTCTTTATAACAGAAGTGTACCTGCATGGGAAGATACATTAGAGAAAATAAAGGAAACACCGCAAAAGGAAATTCAGGATGTGCTTAGAATAAGCTTTGATGGACTAGAGGCTTCAGAGAAGAACATTTTTCTAGATATTGCATGTTTCCTTAGAGGAAAAGGAAAAGTATATGCAACAAACATTCTGAAGAGTTGTGGCTTCTTTCCTGATATTGGATTACAAGTTCTAGTTGATAAAGCTCTTGTAACTATTAGATATCACGATAGGCTAGAGATGCATGATTTACTTGTGGAACTGGGTCGGAATATAGGGAGAGCCAGTAGGCTGTGGAGTTACGAAGATTATCGTGTGTTAACTGAAAATAAGGCTGAAGCAGTTGAAGCTATAGACCTAGACTTGCGGAACCGGAACATAGAAGAGATATACCGGAACACTGAAGCTTTTCTTAGCATGAAAAATTTAAGACTGCTCAAGATCGTTTTGGGCAACTTTTGTAAACAACACATGATTGGGGACTTACAGTTTCTTTCGCATCAGCTGAGGCTTCTTGAATGGCGTAGTTACCCTCTAAAGTCTTTGCCGTCTAATTTTGAGCCGCTGTATCTTGTCTGCCTTAGCATGCCTCGTGGTCGCATTGAAAAACTATGGGAAGGAACCAAGCCTCTGGGAAACTTGAAATCCATCAATTTAAGTGACTGTCATTACCTAAGAAAAATCCCAGACTGCTCTCAAGCTACAAATCTTGAGACACTAAATCTTAGTGGCTGTACAAGTTTAGTGGAGGTTCATTCATCCATTTCAGCTCTTGAAAACCTTGTTTTCTGGAGTCTAGAAAATTGTAAAGTACTTGAAAGTCTTCCAAGCAGCATTCACATGAAATCTCTGGAAACCCTTATTCTTTCTGGCTGCTCAAACCTTGCGAAGTTTCCAGAGATTGGAGGGATCATGAAGGAGCTATTAAAGCTTTGTTTAGATGAGACTGCAATTGAAGAGTTGCCTTCATCAATTGAAAATCTTACTGGGCTTGTTACTTTGAGTCTAGAAAGGTGCGGAAAACTCAAACAACTTCCTAGCAGCATTCACATGAAGTCTCTGAAAACCCTTAGTCTTTCTGGCTGCTCAAACCTTACGAAGTTTCCAGAGATTGGAGGGATCATGAAGGAGCTATTAAAGCTTTGTTTAGATGAGACTGCAATTGAAGAGTTGTCTTTGTTGATGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

445

Amino Acids

50.37

Weight (kDa)

6.77

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 75 - 144 6.4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 330 - 400 1.1e-12 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 358 - 435 2.9e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 120
Acc36I ACCTGC 1 cut(s) 142
AccB7I CCANNNNNTGG 2 cut(s) 1049, 1259
AciI CCGC 4 cut(s) 173, 514, 710, 1164
AclWI GGATC 2 cut(s) 1064, 1274
AcsI RAATTY 2 cut(s) 183, 571
AcuI CTGAAG 4 cut(s) 210, 320, 504, 570
AfaI GTAC 3 cut(s) 132, 883, 960
AfiI CCNNNNNNNGG 3 cut(s) 326, 1049, 1259
AgsI TTSAA 9 cut(s) 494, 665, 752, 790, 923, 965, 1103, 1124, 1313
AloI GAACNNNNNNTCC 2 cut(s) 321, 353
Alw26I GTCTC 4 cut(s) 854, 1085, 1209, 1295
AlwI GGATC 2 cut(s) 1064, 1274
ApeKI GCWGC 4 cut(s) 978, 1020, 1188, 1230
ApoI RAATTY 2 cut(s) 183, 571
Asp700I GAANNNNTTC 2 cut(s) 555, 969
BauI CACGAG 1 cut(s) 738
BbsI GAAGAC 1 cut(s) 962
BbvCI CCTCAGC 1 cut(s) 653
BbvI GCAGC 4 cut(s) 990, 1007, 1200, 1217
BccI CCATC 2 cut(s) 206, 803
BceAI ACGGC 1 cut(s) 679
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 4 cut(s) 854, 1085, 1209, 1295
BfaI CTAG 8 cut(s) 218, 245, 341, 383, 506, 944, 1154, 1185
BfmI CTRYAG 1 cut(s) 498
BfuAI ACCTGC 1 cut(s) 142
BisI GCNGC 5 cut(s) 710, 979, 1021, 1189, 1231
BlsI GCNGC 5 cut(s) 711, 980, 1022, 1190, 1232
BmcAI AGTACT 1 cut(s) 960
BmiI GGNNCC 2 cut(s) 518, 769
BmrI ACTGGG 2 cut(s) 419, 1143
BmsI GCATC 2 cut(s) 378, 655
BmuI ACTGGG 2 cut(s) 419, 1143
BpiI GAAGAC 1 cut(s) 962
BpmI CTGGAG 1 cut(s) 958
Bpu10I CCTNAGC 2 cut(s) 653, 728
BpuEI CTTGAG 4 cut(s) 60, 569, 828, 878
BsaWI WCCGGW 2 cut(s) 519, 540
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 1049, 1259
Bse1I ACTGG 3 cut(s) 414, 432, 1138
BseGI GGATG 2 cut(s) 196, 905
BseLI CCNNNNNNNGG 3 cut(s) 326, 1049, 1259
BseMII CTCAG 1 cut(s) 644
BseNI ACTGG 3 cut(s) 414, 432, 1138
BseXI GCAGC 4 cut(s) 990, 1007, 1200, 1217
BsiSI CCGG 2 cut(s) 520, 541
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 3 cut(s) 326, 1049, 1259
BsmAI GTCTC 4 cut(s) 854, 1085, 1209, 1295
BsmFI GGGAC 1 cut(s) 641
BsmI GAATGC 2 cut(s) 981, 1191
Bsp1407I TGTACA 1 cut(s) 881
Bsp143I GATC 4 cut(s) 43, 588, 1056, 1266
BspACI CCGC 4 cut(s) 173, 514, 710, 1164
BspCNI CTCAG 1 cut(s) 645
BspHI TCATGA 2 cut(s) 1059, 1269
BspLI GGNNCC 2 cut(s) 518, 769
BspMI ACCTGC 1 cut(s) 142
BspPI GGATC 2 cut(s) 1064, 1274
BsrGI TGTACA 1 cut(s) 881
BsrI ACTGG 3 cut(s) 414, 432, 1138
BssMI GATC 4 cut(s) 43, 588, 1056, 1266
BssSI CACGAG 1 cut(s) 738
Bst2BI CACGAG 1 cut(s) 738
Bst4CI ACNGT 3 cut(s) 30, 636, 812
Bst6I CTCTTC 4 cut(s) 296, 525, 1098, 1308
BstAUI TGTACA 1 cut(s) 881
BstC8I GCNNGC 2 cut(s) 60, 734
BstDEI CTNAG 8 cut(s) 197, 263, 560, 653, 728, 821, 872, 1217
BstF5I GGATG 2 cut(s) 196, 905
BstKTI GATC 4 cut(s) 46, 591, 1059, 1269
BstMAI GTCTC 4 cut(s) 854, 1085, 1209, 1295
BstMBI GATC 4 cut(s) 43, 588, 1056, 1266
BstMWI GCNNNNNNNGC 4 cut(s) 388, 494, 1075, 1285
BstNSI RCATGY 2 cut(s) 258, 736
BstSFI CTRYAG 1 cut(s) 498
BstV1I GCAGC 4 cut(s) 990, 1007, 1200, 1217
BstV2I GAAGAC 1 cut(s) 962
BstXI CCANNNNNNTGG 1 cut(s) 778
BtsCI GGATG 2 cut(s) 196, 905
BtsIMutI CAGTG 1 cut(s) 546
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 60, 734
CciI TCATGA 2 cut(s) 1059, 1269
Csp6I GTAC 3 cut(s) 131, 882, 959
CspCI CAANNNNNGTGG 2 cut(s) 873, 908
CviQI GTAC 3 cut(s) 131, 882, 959
DdeI CTNAG 8 cut(s) 197, 263, 560, 653, 728, 821, 872, 1217
DpnI GATC 4 cut(s) 45, 590, 1058, 1268
DpnII GATC 4 cut(s) 43, 588, 1056, 1266
Eam1104I CTCTTC 4 cut(s) 296, 525, 1098, 1308
EarI CTCTTC 4 cut(s) 296, 525, 1098, 1308
Eco32I GATATC 1 cut(s) 371
Eco57I CTGAAG 4 cut(s) 210, 320, 504, 570
EcoRV GATATC 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 393
FalI AAGNNNNNCTT 3 cut(s) 28, 543, 575
FaqI GGGAC 1 cut(s) 641
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 5 cut(s) 710, 979, 1021, 1189, 1231
FokI GGATG 2 cut(s) 203, 892
Fsp4HI GCNGC 5 cut(s) 710, 979, 1021, 1189, 1231
FspBI CTAG 8 cut(s) 218, 245, 341, 383, 506, 944, 1154, 1185
GluI GCNGC 5 cut(s) 710, 979, 1021, 1189, 1231
GsuI CTGGAG 1 cut(s) 958
HapII CCGG 2 cut(s) 520, 541
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HindIII AAGCTT 4 cut(s) 205, 552, 1076, 1286
HinfI GANTC 2 cut(s) 940, 1150
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 2 cut(s) 520, 541
Hpy166II GTNNAC 3 cut(s) 131, 468, 611
Hpy188I TCNGA 5 cut(s) 17, 229, 300, 417, 1209
Hpy8I GTNNAC 3 cut(s) 131, 468, 611
HpyAV CCTTC 5 cut(s) 22, 758, 1057, 1122, 1267
HpyCH4III ACNGT 3 cut(s) 30, 636, 812
HpyCH4V TGCA 6 cut(s) 137, 254, 287, 391, 1097, 1307
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 494, 1075, 1285
HpyF3I CTNAG 8 cut(s) 197, 263, 560, 653, 728, 821, 872, 1217
Ksp22I TGATCA 1 cut(s) 43
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 4 cut(s) 43, 588, 1056, 1266
LmnI GCTCC 3 cut(s) 107, 1066, 1276
Lsp1109I GCAGC 4 cut(s) 990, 1007, 1200, 1217
LweI GCATC 2 cut(s) 378, 655
MaeI CTAG 8 cut(s) 218, 245, 341, 383, 506, 944, 1154, 1185
MaeIII GTNAC 5 cut(s) 358, 446, 674, 806, 1141
MalI GATC 4 cut(s) 45, 590, 1058, 1268
MboI GATC 4 cut(s) 43, 588, 1056, 1266
MboII GAAGA 8 cut(s) 155, 244, 313, 464, 542, 962, 1115, 1325
MfeI CAATTG 3 cut(s) 1098, 1119, 1308
MluCI AATT 8 cut(s) 183, 571, 700, 799, 949, 1098, 1119, 1308
MlyI GAGTC 2 cut(s) 949, 1159
MmeI TCCRAC 1 cut(s) 395
MnlI CCTC 9 cut(s) 214, 260, 648, 690, 747, 786, 889, 1046, 1256
Mph1103I ATGCAT 1 cut(s) 393
MroXI GAANNNNTTC 2 cut(s) 555, 969
MseI TTAA 6 cut(s) 96, 467, 575, 803, 1073, 1283
MslI CAYNNNNRTG 2 cut(s) 986, 1196
MspA1I CMGCKG 2 cut(s) 652, 712
MspI CCGG 2 cut(s) 520, 541
MunI CAATTG 3 cut(s) 1098, 1119, 1308
Mva1269I GAATGC 2 cut(s) 981, 1191
MwoI GCNNNNNNNGC 4 cut(s) 388, 494, 1075, 1285
NdeII GATC 4 cut(s) 43, 588, 1056, 1266
NlaIV GGNNCC 2 cut(s) 518, 769
NmuCI GTSAC 1 cut(s) 806
NsiI ATGCAT 1 cut(s) 393
NspI RCATGY 2 cut(s) 258, 736
PaeI GCATGC 1 cut(s) 736
PagI TCATGA 2 cut(s) 1059, 1269
PctI GAATGC 2 cut(s) 981, 1191
PdmI GAANNNNTTC 2 cut(s) 555, 969
PflMI CCANNNNNTGG 2 cut(s) 1049, 1259
PkrI GCNGC 5 cut(s) 711, 980, 1022, 1190, 1232
PleI GAGTC 2 cut(s) 948, 1158
PpsI GAGTC 2 cut(s) 948, 1158
PsiI TTATAA 1 cut(s) 120
PspN4I GGNNCC 2 cut(s) 518, 769
PvuII CAGCTG 1 cut(s) 652
RsaI GTAC 3 cut(s) 132, 883, 960
RsaNI GTAC 3 cut(s) 131, 882, 959
RseI CAYNNNNRTG 2 cut(s) 986, 1196
SaqAI TTAA 6 cut(s) 96, 467, 575, 803, 1073, 1283
SatI GCNGC 5 cut(s) 710, 979, 1021, 1189, 1231
Sau3AI GATC 4 cut(s) 43, 588, 1056, 1266
ScaI AGTACT 1 cut(s) 960
SchI GAGTC 2 cut(s) 949, 1159
SfaNI GCATC 2 cut(s) 378, 655
SfcI CTRYAG 1 cut(s) 498
SmiMI CAYNNNNRTG 2 cut(s) 986, 1196
SmlI CTYRAG 4 cut(s) 75, 584, 843, 857
SmoI CTYRAG 4 cut(s) 75, 584, 843, 857
SphI GCATGC 1 cut(s) 736
Sse9I AATT 8 cut(s) 183, 571, 700, 799, 949, 1098, 1119, 1308
SsiI CCGC 4 cut(s) 173, 514, 710, 1164
SspMI CTAG 8 cut(s) 218, 245, 341, 383, 506, 944, 1154, 1185
TaaI ACNGT 3 cut(s) 30, 636, 812
TasI AATT 8 cut(s) 183, 571, 700, 799, 949, 1098, 1119, 1308
TatI WGTACW 2 cut(s) 881, 958
TauI GCSGC 1 cut(s) 712
Tru1I TTAA 6 cut(s) 96, 467, 575, 803, 1073, 1283
Tru9I TTAA 6 cut(s) 96, 467, 575, 803, 1073, 1283
TscAI CASTG 1 cut(s) 553
TseFI GTSAC 1 cut(s) 806
TseI GCWGC 4 cut(s) 978, 1020, 1188, 1230
Tsp45I GTSAC 1 cut(s) 806
TspRI CASTG 1 cut(s) 553
Van91I CCANNNNNTGG 2 cut(s) 1049, 1259
XapI RAATTY 2 cut(s) 183, 571
XbaI TCTAGA 3 cut(s) 244, 943, 1153
XceI RCATGY 2 cut(s) 258, 736
XmnI GAANNNNTTC 2 cut(s) 555, 969
XspI CTAG 8 cut(s) 218, 245, 341, 383, 506, 944, 1154, 1185
ZrmI AGTACT 1 cut(s) 960
Zsp2I ATGCAT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.