Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0001306

Physical Location & Seq

Genomic Coordinates Reverse (-)

41542304 .. 41546515

Gene Structure 4212 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1339900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGGCCTCAAAATCTGGTACCAGAGCTTCCTCAAGAATGCAACAGAAAGCAGCTGGTGGGCAGACGGTAGAAACTAATGATCTCAGGCAATTGCTCCCGGAGCAATTACCAAAATCTGTACAAAACTCATCAAAGCAGGTTGCAGTAGACGCTTCTGATAATAAAAGGTCAACAAATTCTAATGAACAAGGATCTGCGGATTCCTTGATTAGTAAATTGAATTCAATTTCATTGTCAATGTCATCCAACCTTGAAAAGGCAACAAGGGAAATAGATTCTACTGCAAATGAGACTAGAGGCTCACTTGAAAGTTCTGTTGATCAAGAAAAGAAGACATCAGAGTATGGAAGTGTGAAGGACAGTTCAGTTTCGGCCAAAGTGAGCGATGGAACAAGCAGCCTTGCCAAGACCAGTGGAAGTGCTAAGATTAGTGATCGAGTTGATTTTGTTGAGAGTGGCAAGAGCAGTATGTGTAGAGGAAGCACTGGCAGTGATGTGAGTGATGAGAGCACCTGTAGCAGCTTTAGTAGCAGCATCAGCAAACCTCACAAAGCAAATGACTTGAGATGGGAAGCTATTCAAGCTGTCCGAACTAGAGATGGGGCTCTGGGTCTGGGTCATTTCAGACTGCTGAAGAGGTTAGGCTGTGGGGATATTGGAAGTGTCTATCTGTCAGAGCTGAGTGGAACAAAATGTTATTTTGCAATGAAAGTTATGGACAAAGGGTCTCTAGCAAGTCGTAAAAAGCTTCTTCGGGCTCAGACAGAAAGAGAGATACTGCAATCTCTGGATCACCCCTTCCTTCCAACATTATATACCCACTTTGAGACAGACAAGTTTTCATGCTTGGTAATAGAGTTCTGTCCTGGAGGAGACTTGCACACTCTCAGGCAGAGGCAGCCAGGGAAGCATTTTTCTGAGCAGGCAGTAAAATTCTATGTTGCTGAGGTCCTGCTATCTCTGGAATACCTCCACATGCTTGGGATTGTCTACCGTGACCTTAAGCCGGAAAATGTGCTAGTGAGGGAAGATGGTCATATAATGCTCTCTGACTTTGATCTTTCCCTCCGTTGTGCTGTTAGTCCAACTCTTGTCAAAACATCATCAATGGAGTTTGAACCCTTGCGCAAGAACCCTGTGTATTGTGTCCAGCCAGCTTGCATTGAGCCACCTTCCTGTATCCAACCATCCTGTGTAGCTCCTACAACATGCTTTGGACCACGTCTGTTTTCTAGCAAGTCCAAGAAGGACAAAAAATCTAAAAACGAAATAGGAAACCAAGTTAGACCATTACCTGAGCTGATTGCTGAGCCAACTGATGCCAGGTCTATGTCATTTGTTGGAACCCATGAATATTTGGCACCTGAGATCATCAAGGGGGAAGGTCATGGAAGTGCTGTTGATTGGTGGACTTTTGGAATCTTTTTATATGAGCTGTTGTTTGGTAAAACTCCATTTAAAGGTTCCGGGAATCGTGCAACATTGTTCAATGTTGTGGGTCAACCTCTCCGGTTTCCAGAATCACCAGTTGTCAGTTTTGCAGCAAGGGATCTCATAAGGGGATTGCTTGTTAAGGAACCGCAGCACAGATTGGCATATAAACGAGGGGCAACTGAGATTAAGCAACATCCCTTCTTTGAAGGTGTAAATTGGGCATTGATACGTTGTGCTACCCCACCGGATATCCCAAAGCCTGTTGAGATTGAGCGGATACCAGCCCCAACAGCATCATCAAGCGAGAAGGAGAAGGCTCCTCCCACCCCCACAATCGCTTCTCCCGTTCAAAAGGGTTCTGATAATTATCTGGAGTTCGATTTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

608

Amino Acids

66.64

Weight (kDa)

8.52

Isoelectric Point (pI)

44.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	209 - 359	3.4e-35	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	209 - 359	1.2e-18	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	443 - 546	2.1e-20	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	450 - 533	6.6e-06	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26610 AT5G47750 AT5G55910 AT5G55910
fragaria_vesca	FvH4_3g00260 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710 FvH4_3g42710
malus_domestica	MD03G1029500.v1.1 MD09G1025300.v1.1 MD10G1339900.v1.1 MD11G1032900.v1.1
prunus_persica	Prupe.4G002900_v2.0.a1 Prupe.4G002900_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1 Prupe.6G025600_v2.0.a1
pyrus_communis	pycom03g02440 pycom10g28980 pycom11g02860
rosa_chinensis	RchiOBHm_Chr5g0000381 RchiOBHm_Chr5g0076451
rosa_laevigata	RLG00000030861 RLG00000030862 RLG00000036620
rosa_multiflora	Rmu_co8411395.1_g000001 Rmu_sc0000369.1_g000018 Rmu_sc0001680.1_g000004 Rmu_ssc0000181.1_g000002 Rmu_ssc0000385.1_g000014
rosa_roxburghii	Rroxscaffold_1G00075770 Rroxscaffold_1G00075780
rosa_rugosa	Rorug04G0383300 Rorug04G0383300 Rorug05G0447900 Rorug05G0448000 Rorug05G0448100
rosa_samantha	Rh5AG003200 Rh5AG502800 Rh5BG004200 Rh5BG524500 Rh5CG003400 Rh5CG548100 Rh5DG003100 Rh5DG003200 Rh5DG536300 Rh7BG161800
rosa_wichuraiana	Rw5G000310 Rw5G046640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1127
Acc36I	ACCTGC	1 cut(s)	127
Acc65I	GGTACC	1 cut(s)	17
AccB1I	GGYRCC	2 cut(s)	17, 1360
AccBSI	CCGCTC	1 cut(s)	1708
AccI	GTMKAC	2 cut(s)	147, 990
AciI	CCGC	3 cut(s)	197, 1580, 1708
AclWI	GGATC	3 cut(s)	199, 798, 1557
AcoI	YGGCCR	1 cut(s)	372
AcsI	RAATTY	3 cut(s)	175, 220, 932
AcuI	CTGAAG	1 cut(s)	653
AfaI	GTAC	2 cut(s)	19, 120
AfiI	CCNNNNNNNGG	3 cut(s)	256, 1006, 1460
AflII	CTTAAG	1 cut(s)	1001
AgsI	TTSAA	9 cut(s)	220, 225, 254, 308, 581, 1118, 1489, 1640, 1784
AhdI	GACNNNNNGTC	1 cut(s)	724
AjiI	CACGTC	1 cut(s)	1223
AjnI	CCWGG	3 cut(s)	865, 901, 1322
Alw21I	GWGCWC	1 cut(s)	512
Alw26I	GTCTC	4 cut(s)	284, 732, 821, 867
AlwI	GGATC	3 cut(s)	199, 798, 1557
AoxI	GGCC	2 cut(s)	3, 372
ApeKI	GCWGC	7 cut(s)	50, 396, 519, 531, 898, 1541, 1582
ApoI	RAATTY	3 cut(s)	175, 220, 932
Asp700I	GAANNNNTTC	1 cut(s)	576
Asp718I	GGTACC	1 cut(s)	17
AspLEI	GCGC	1 cut(s)	1128
AspS9I	GGNCC	2 cut(s)	949, 1217
AsuC2I	CCSGG	2 cut(s)	98, 1468
AsuHPI	GGTGA	2 cut(s)	785, 1515
AvaII	GGWCC	2 cut(s)	949, 1217
BanI	GGYRCC	2 cut(s)	17, 1360
BanII	GRGCYC	2 cut(s)	607, 760
BarI	GAAGNNNNNNTAC	2 cut(s)	10, 42
BbsI	GAAGAC	1 cut(s)	338
Bbv12I	GWGCWC	1 cut(s)	512
BbvCI	CCTCAGC	1 cut(s)	945
BbvI	GCAGC	7 cut(s)	62, 408, 531, 543, 910, 1553, 1594
BccI	CCATC	5 cut(s)	380, 561, 593, 1025, 1195
BciT130I	CCWGG	3 cut(s)	867, 903, 1324
BciVI	GTATCC	2 cut(s)	1190, 1704
BclI	TGATCA	1 cut(s)	319
BcnI	CCSGG	2 cut(s)	98, 1468
BcoDI	GTCTC	4 cut(s)	284, 732, 821, 867
BfaI	CTAG	5 cut(s)	294, 594, 731, 1019, 1233
BfmI	CTRYAG	1 cut(s)	514
BfrI	CTTAAG	1 cut(s)	1001
BfuAI	ACCTGC	1 cut(s)	127
BfuI	GTATCC	2 cut(s)	1190, 1704
BisI	GCNGC	7 cut(s)	51, 397, 520, 532, 899, 1542, 1583
BlpI	GCTNAGC	1 cut(s)	1308
BlsI	GCNGC	7 cut(s)	52, 398, 521, 533, 900, 1543, 1584
Bme1390I	CCNGG	5 cut(s)	98, 867, 903, 1324, 1468
Bme18I	GGWCC	2 cut(s)	949, 1217
BmeRI	GACNNNNNGTC	1 cut(s)	724
BmgBI	CACGTC	1 cut(s)	1223
BmgT120I	GGNCC	2 cut(s)	949, 1217
BmiI	GGNNCC	6 cut(s)	19, 1345, 1362, 1465, 1578, 1752
BmrFI	CCNGG	5 cut(s)	98, 867, 903, 1324, 1468
BmsI	GCATC	3 cut(s)	543, 1309, 1736
BpiI	GAAGAC	1 cut(s)	338
BpmI	CTGGAG	1 cut(s)	888
Bpu10I	CCTNAGC	2 cut(s)	945, 1296
Bpu1102I	GCTNAGC	1 cut(s)	1308
BpuEI	CTTGAG	2 cut(s)	16, 583
BpuMI	CCSGG	2 cut(s)	98, 1468
BsaBI	GATNNNNATC	1 cut(s)	1800
BsaI	GGTCTC	1 cut(s)	732
BsaJI	CCNNGG	1 cut(s)	902
BsaWI	WCCGGW	2 cut(s)	1509, 1678
Bsc4I	CCNNNNNNNGG	3 cut(s)	256, 1006, 1460
Bse1I	ACTGG	3 cut(s)	411, 490, 1526
Bse3DI	GCAATG	1 cut(s)	711
Bse8I	GATNNNNATC	1 cut(s)	1800
BseBI	CCWGG	3 cut(s)	867, 903, 1324
BseDI	CCNNGG	1 cut(s)	902
BseGI	GGATG	3 cut(s)	242, 1187, 1627
BseJI	GATNNNNATC	1 cut(s)	1800
BseLI	CCNNNNNNNGG	3 cut(s)	256, 1006, 1460
BseMI	GCAATG	1 cut(s)	711
BseNI	ACTGG	3 cut(s)	411, 490, 1526
BseRI	GAGGAG	2 cut(s)	885, 1743
BseXI	GCAGC	7 cut(s)	62, 408, 531, 543, 910, 1553, 1594
BshFI	GGCC	2 cut(s)	5, 374
BshNI	GGYRCC	2 cut(s)	17, 1360
BsiHKAI	GWGCWC	1 cut(s)	512
BsiSI	CCGG	5 cut(s)	98, 1007, 1467, 1510, 1679
BslI	CCNNNNNNNGG	3 cut(s)	256, 1006, 1460
BsmAI	GTCTC	4 cut(s)	284, 732, 821, 867
BsmI	GAATGC	1 cut(s)	42
BsnI	GGCC	2 cut(s)	5, 374
Bso31I	GGTCTC	1 cut(s)	732
Bsp1286I	GDGCHC	3 cut(s)	512, 607, 760
Bsp1407I	TGTACA	1 cut(s)	118
Bsp143I	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
Bsp1720I	GCTNAGC	1 cut(s)	1308
BspACI	CCGC	3 cut(s)	197, 1580, 1708
BspANI	GGCC	2 cut(s)	5, 374
BspLI	GGNNCC	6 cut(s)	19, 1345, 1362, 1465, 1578, 1752
BspMI	ACCTGC	1 cut(s)	127
BspPI	GGATC	3 cut(s)	199, 798, 1557
BspT107I	GGYRCC	2 cut(s)	17, 1360
BspTI	CTTAAG	1 cut(s)	1001
BspTNI	GGTCTC	1 cut(s)	732
BsrBI	CCGCTC	1 cut(s)	1708
BsrDI	GCAATG	1 cut(s)	711
BsrGI	TGTACA	1 cut(s)	118
BsrI	ACTGG	3 cut(s)	411, 490, 1526
BssECI	CCNNGG	1 cut(s)	902
BssMI	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
Bst2UI	CCWGG	3 cut(s)	867, 903, 1324
Bst4CI	ACNGT	3 cut(s)	67, 362, 995
Bst6I	CTCTTC	1 cut(s)	629
BstAFI	CTTAAG	1 cut(s)	1001
BstAUI	TGTACA	1 cut(s)	118
BstC8I	GCNNGC	3 cut(s)	924, 1155, 1159
BstENI	CCTNNNNNAGG	1 cut(s)	254
BstF5I	GGATG	3 cut(s)	242, 1187, 1627
BstHHI	GCGC	1 cut(s)	1128
BstKTI	GATC	8 cut(s)	82, 194, 322, 436, 793, 1060, 1371, 1552
BstMAI	GTCTC	4 cut(s)	284, 732, 821, 867
BstMBI	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
BstMWI	GCNNNNNNNGC	9 cut(s)	100, 149, 516, 528, 537, 581, 898, 907, 1724
BstNI	CCWGG	3 cut(s)	867, 903, 1324
BstNSI	RCATGY	2 cut(s)	979, 1212
BstSCI	CCNGG	5 cut(s)	96, 865, 901, 1322, 1466
BstSFI	CTRYAG	1 cut(s)	514
BstV1I	GCAGC	7 cut(s)	62, 408, 531, 543, 910, 1553, 1594
BstV2I	GAAGAC	1 cut(s)	338
BstX2I	RGATCY	2 cut(s)	191, 1549
BstXI	CCANNNNNNTGG	1 cut(s)	980
BstYI	RGATCY	2 cut(s)	191, 1549
BsuI	GTATCC	2 cut(s)	1190, 1704
BsuRI	GGCC	2 cut(s)	5, 374
BtgZI	GCGATG	1 cut(s)	399
BtrI	CACGTC	1 cut(s)	1223
BtsCI	GGATG	3 cut(s)	242, 1187, 1627
BtsI	GCAGTG	1 cut(s)	496
BtsIMutI	CAGTG	3 cut(s)	418, 483, 496
BveI	ACCTGC	1 cut(s)	127
Cac8I	GCNNGC	3 cut(s)	924, 1155, 1159
CfoI	GCGC	1 cut(s)	1128
Cfr13I	GGNCC	2 cut(s)	949, 1217
CseI	GACGC	1 cut(s)	158
Csp6I	GTAC	2 cut(s)	18, 119
CspCI	CAANNNNNGTGG	2 cut(s)	394, 429
CviAII	CATG	5 cut(s)	843, 976, 1209, 1349, 1388
CviQI	GTAC	2 cut(s)	18, 119
DpnI	GATC	8 cut(s)	81, 193, 321, 435, 792, 1059, 1370, 1551
DpnII	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
DraI	TTTAAA	1 cut(s)	1459
DriI	GACNNNNNGTC	1 cut(s)	724
EaeI	YGGCCR	1 cut(s)	372
Eam1104I	CTCTTC	1 cut(s)	629
Eam1105I	GACNNNNNGTC	1 cut(s)	724
EarI	CTCTTC	1 cut(s)	629
Eco24I	GRGCYC	2 cut(s)	607, 760
Eco31I	GGTCTC	1 cut(s)	732
Eco32I	GATATC	1 cut(s)	1684
Eco47I	GGWCC	2 cut(s)	949, 1217
Eco57I	CTGAAG	1 cut(s)	653
EcoNI	CCTNNNNNAGG	1 cut(s)	254
EcoO109I	RGGNCCY	1 cut(s)	949
EcoRI	GAATTC	1 cut(s)	220
EcoRII	CCWGG	3 cut(s)	865, 901, 1322
EcoRV	GATATC	1 cut(s)	1684
EcoT38I	GRGCYC	2 cut(s)	607, 760
FaeI	CATG	5 cut(s)	846, 979, 1212, 1352, 1391
FatI	CATG	5 cut(s)	842, 975, 1208, 1348, 1387
FbaI	TGATCA	1 cut(s)	319
FblI	GTMKAC	2 cut(s)	147, 990
Fnu4HI	GCNGC	7 cut(s)	51, 397, 520, 532, 899, 1542, 1583
FokI	GGATG	3 cut(s)	229, 1174, 1614
FriOI	GRGCYC	2 cut(s)	607, 760
Fsp4HI	GCNGC	7 cut(s)	51, 397, 520, 532, 899, 1542, 1583
FspBI	CTAG	5 cut(s)	294, 594, 731, 1019, 1233
FspI	TGCGCA	1 cut(s)	1127
GlaI	GCGC	1 cut(s)	1127
GluI	GCNGC	7 cut(s)	51, 397, 520, 532, 899, 1542, 1583
GsuI	CTGGAG	1 cut(s)	888
HaeIII	GGCC	2 cut(s)	5, 374
HapII	CCGG	5 cut(s)	98, 1007, 1467, 1510, 1679
HgaI	GACGC	1 cut(s)	158
HhaI	GCGC	1 cut(s)	1128
Hin1II	CATG	5 cut(s)	846, 979, 1212, 1352, 1391
Hin6I	GCGC	1 cut(s)	1126
HinP1I	GCGC	1 cut(s)	1126
HincII	GTYRAC	2 cut(s)	171, 1502
HindII	GTYRAC	2 cut(s)	171, 1502
HindIII	AAGCTT	1 cut(s)	746
HinfI	GANTC	5 cut(s)	200, 275, 1419, 1471, 1520
HpaII	CCGG	5 cut(s)	98, 1007, 1467, 1510, 1679
HphI	GGTGA	2 cut(s)	785, 1515
Hpy166II	GTNNAC	5 cut(s)	148, 171, 991, 1410, 1502
Hpy188I	TCNGA	9 cut(s)	157, 340, 590, 626, 676, 762, 919, 1051, 1795
Hpy188III	TCNNGA	6 cut(s)	33, 323, 788, 962, 1517, 1805
Hpy8I	GTNNAC	5 cut(s)	148, 171, 991, 1410, 1502
HpyCH4III	ACNGT	3 cut(s)	67, 362, 995
HpyCH4IV	ACGT	2 cut(s)	1222, 1663
HpyCH4V	TGCA	9 cut(s)	40, 143, 284, 704, 781, 880, 1161, 1478, 1541
HpyF10VI	GCNNNNNNNGC	9 cut(s)	100, 149, 516, 528, 537, 581, 898, 907, 1724
HpySE526I	ACGT	2 cut(s)	1222, 1663
Hsp92II	CATG	5 cut(s)	846, 979, 1212, 1352, 1391
HspAI	GCGC	1 cut(s)	1126
KpnI	GGTACC	1 cut(s)	21
Ksp22I	TGATCA	1 cut(s)	319
Kzo9I	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
LmnI	GCTCC	4 cut(s)	99, 100, 1204, 1756
Lsp1109I	GCAGC	7 cut(s)	62, 408, 531, 543, 910, 1553, 1594
LweI	GCATC	3 cut(s)	543, 1309, 1736
MaeI	CTAG	5 cut(s)	294, 594, 731, 1019, 1233
MaeII	ACGT	2 cut(s)	1222, 1663
MaeIII	GTNAC	1 cut(s)	995
MalI	GATC	8 cut(s)	81, 193, 321, 435, 792, 1059, 1370, 1551
MbiI	CCGCTC	1 cut(s)	1708
MboI	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
MboII	GAAGA	4 cut(s)	343, 646, 743, 1040
MfeI	CAATTG	1 cut(s)	89
MflI	RGATCY	2 cut(s)	191, 1549
MhlI	GDGCHC	3 cut(s)	512, 607, 760
MluCI	AATT	9 cut(s)	89, 104, 175, 215, 220, 225, 932, 1648, 1798
MmeI	TCCRAC	5 cut(s)	270, 830, 1109, 1207, 1321
MroXI	GAANNNNTTC	1 cut(s)	576
MseI	TTAA	4 cut(s)	1002, 1458, 1572, 1620
MslI	CAYNNNNRTG	1 cut(s)	1392
MspA1I	CMGCKG	1 cut(s)	53
MspCI	CTTAAG	1 cut(s)	1001
MspI	CCGG	5 cut(s)	98, 1007, 1467, 1510, 1679
MspR9I	CCNGG	5 cut(s)	98, 867, 903, 1324, 1468
MunI	CAATTG	1 cut(s)	89
Mva1269I	GAATGC	1 cut(s)	42
MvaI	CCWGG	3 cut(s)	867, 903, 1324
MwoI	GCNNNNNNNGC	9 cut(s)	100, 149, 516, 528, 537, 581, 898, 907, 1724
NciI	CCSGG	2 cut(s)	98, 1468
NdeII	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
NlaIII	CATG	5 cut(s)	846, 979, 1212, 1352, 1391
NlaIV	GGNNCC	6 cut(s)	19, 1345, 1362, 1465, 1578, 1752
NmuCI	GTSAC	1 cut(s)	995
NsbI	TGCGCA	1 cut(s)	1127
NspI	RCATGY	2 cut(s)	979, 1212
PcsI	WCGNNNNNNNCGW	1 cut(s)	1776
PctI	GAATGC	1 cut(s)	42
PdmI	GAANNNNTTC	1 cut(s)	576
PfeI	GAWTC	5 cut(s)	200, 275, 1419, 1471, 1520
PflFI	GACNNNGTC	1 cut(s)	1221
PfoI	TCCNGGA	3 cut(s)	96, 865, 1466
PkrI	GCNGC	7 cut(s)	52, 398, 521, 533, 900, 1543, 1584
PpuMI	RGGWCCY	1 cut(s)	949
Psp5II	RGGWCCY	1 cut(s)	949
Psp6I	CCWGG	3 cut(s)	865, 901, 1322
PspGI	CCWGG	3 cut(s)	865, 901, 1322
PspN4I	GGNNCC	6 cut(s)	19, 1345, 1362, 1465, 1578, 1752
PspPI	GGNCC	2 cut(s)	949, 1217
PspPPI	RGGWCCY	1 cut(s)	949
PsuI	RGATCY	2 cut(s)	191, 1549
PsyI	GACNNNGTC	1 cut(s)	1221
PvuII	CAGCTG	1 cut(s)	53
RsaI	GTAC	2 cut(s)	19, 120
RsaNI	GTAC	2 cut(s)	18, 119
RseI	CAYNNNNRTG	1 cut(s)	1392
SaqAI	TTAA	4 cut(s)	1002, 1458, 1572, 1620
SatI	GCNGC	7 cut(s)	51, 397, 520, 532, 899, 1542, 1583
Sau3AI	GATC	8 cut(s)	79, 191, 319, 433, 790, 1057, 1368, 1549
Sau96I	GGNCC	2 cut(s)	949, 1217
ScrFI	CCNGG	5 cut(s)	98, 867, 903, 1324, 1468
SduI	GDGCHC	3 cut(s)	512, 607, 760
SfaNI	GCATC	3 cut(s)	543, 1309, 1736
SfcI	CTRYAG	1 cut(s)	514
SinI	GGWCC	2 cut(s)	949, 1217
SmiMI	CAYNNNNRTG	1 cut(s)	1392
SmlI	CTYRAG	3 cut(s)	31, 562, 1001
SmoI	CTYRAG	3 cut(s)	31, 562, 1001
Sse9I	AATT	9 cut(s)	89, 104, 175, 215, 220, 225, 932, 1648, 1798
SsiI	CCGC	3 cut(s)	197, 1580, 1708
SspI	AATATT	1 cut(s)	1355
SspMI	CTAG	5 cut(s)	294, 594, 731, 1019, 1233
StyD4I	CCNGG	5 cut(s)	96, 865, 901, 1322, 1466
TaaI	ACNGT	3 cut(s)	67, 362, 995
TaiI	ACGT	2 cut(s)	1225, 1666
TaqI	TCGA	2 cut(s)	436, 1812
TasI	AATT	9 cut(s)	89, 104, 175, 215, 220, 225, 932, 1648, 1798
TatI	WGTACW	1 cut(s)	118
TfiI	GAWTC	5 cut(s)	200, 275, 1419, 1471, 1520
Tru1I	TTAA	4 cut(s)	1002, 1458, 1572, 1620
Tru9I	TTAA	4 cut(s)	1002, 1458, 1572, 1620
TscAI	CASTG	3 cut(s)	418, 490, 496
TseFI	GTSAC	1 cut(s)	995
TseI	GCWGC	7 cut(s)	50, 396, 519, 531, 898, 1541, 1582
Tsp45I	GTSAC	1 cut(s)	995
TspDTI	ATGAA	5 cut(s)	198, 219, 722, 831, 1365
TspGWI	ACGGA	1 cut(s)	1058
TspRI	CASTG	3 cut(s)	418, 490, 496
Tth111I	GACNNNGTC	1 cut(s)	1221
Vha464I	CTTAAG	1 cut(s)	1001
VpaK11BI	GGWCC	2 cut(s)	949, 1217
XagI	CCTNNNNNAGG	1 cut(s)	254
XapI	RAATTY	3 cut(s)	175, 220, 932
XceI	RCATGY	2 cut(s)	979, 1212
XmiI	GTMKAC	2 cut(s)	147, 990
XmnI	GAANNNNTTC	1 cut(s)	576
XspI	CTAG	5 cut(s)	294, 594, 731, 1019, 1233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.