Serine/Threonine protein kinases, catalytic domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1329, 1367 |
| AccB1I | GGYRCC | 1 cut(s) | 304 |
| AccI | GTMKAC | 2 cut(s) | 934, 1398 |
| AccII | CGCG | 1 cut(s) | 255 |
| AciI | CCGC | 1 cut(s) | 1048 |
| AcoI | YGGCCR | 1 cut(s) | 320 |
| AcsI | RAATTY | 4 cut(s) | 184, 523, 780, 1185 |
| AfaI | GTAC | 5 cut(s) | 616, 658, 871, 893, 1254 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1175 |
| AflII | CTTAAG | 1 cut(s) | 947 |
| AflIII | ACRYGT | 3 cut(s) | 7, 253, 1288 |
| AjnI | CCWGG | 2 cut(s) | 814, 1300 |
| AluBI | AGCT | 7 cut(s) | 74, 165, 265, 536, 701, 839, 1211 |
| AluI | AGCT | 7 cut(s) | 74, 165, 265, 536, 701, 839, 1211 |
| Alw21I | GWGCWC | 2 cut(s) | 152, 849 |
| AoxI | GGCC | 2 cut(s) | 202, 320 |
| ApeKI | GCWGC | 2 cut(s) | 907, 1045 |
| ApoI | RAATTY | 4 cut(s) | 184, 523, 780, 1185 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 573 |
| AspS9I | GGNCC | 2 cut(s) | 202, 1225 |
| AsuHPI | GGTGA | 3 cut(s) | 321, 563, 1441 |
| AsuII | TTCGAA | 1 cut(s) | 632 |
| AvaII | GGWCC | 1 cut(s) | 1225 |
| BalI | TGGCCA | 1 cut(s) | 322 |
| BanI | GGYRCC | 1 cut(s) | 304 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 375, 407 |
| Bbv12I | GWGCWC | 2 cut(s) | 152, 849 |
| BbvI | GCAGC | 2 cut(s) | 919, 1032 |
| BccI | CCATC | 5 cut(s) | 36, 247, 331, 340, 470 |
| BceAI | ACGGC | 1 cut(s) | 318 |
| BciT130I | CCWGG | 2 cut(s) | 816, 1302 |
| BclI | TGATCA | 2 cut(s) | 400, 1021 |
| BfaI | CTAG | 7 cut(s) | 468, 582, 611, 768, 1067, 1094, 1148 |
| BfrI | CTTAAG | 1 cut(s) | 947 |
| BglII | AGATCT | 1 cut(s) | 1199 |
| BisI | GCNGC | 2 cut(s) | 908, 1046 |
| BlsI | GCNGC | 2 cut(s) | 909, 1047 |
| Bme1390I | CCNGG | 2 cut(s) | 816, 1302 |
| Bme18I | GGWCC | 1 cut(s) | 1225 |
| BmgT120I | GGNCC | 2 cut(s) | 202, 1225 |
| BmiI | GGNNCC | 2 cut(s) | 306, 1226 |
| BmrFI | CCNGG | 2 cut(s) | 816, 1302 |
| BmrI | ACTGGG | 1 cut(s) | 728 |
| BmsI | GCATC | 1 cut(s) | 296 |
| BmuI | ACTGGG | 1 cut(s) | 728 |
| Bpu14I | TTCGAA | 1 cut(s) | 632 |
| BpuEI | CTTGAG | 2 cut(s) | 28, 539 |
| BsaAI | YACGTR | 1 cut(s) | 983 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1338 |
| BsaJI | CCNNGG | 1 cut(s) | 814 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1175 |
| Bse1I | ACTGG | 2 cut(s) | 598, 723 |
| Bse3DI | GCAATG | 1 cut(s) | 508 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1338 |
| BseBI | CCWGG | 2 cut(s) | 816, 1302 |
| BseDI | CCNNGG | 1 cut(s) | 814 |
| BseGI | GGATG | 1 cut(s) | 239 |
| BseJI | GATNNNNATC | 1 cut(s) | 1338 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1175 |
| BseMI | GCAATG | 1 cut(s) | 508 |
| BseMII | CTCAG | 2 cut(s) | 156, 1211 |
| BseNI | ACTGG | 2 cut(s) | 598, 723 |
| BseXI | GCAGC | 2 cut(s) | 919, 1032 |
| BseYI | CCCAGC | 1 cut(s) | 199 |
| BsgI | GTGCAG | 2 cut(s) | 623, 1392 |
| Bsh1236I | CGCG | 1 cut(s) | 255 |
| BshFI | GGCC | 2 cut(s) | 204, 322 |
| BshNI | GGYRCC | 1 cut(s) | 304 |
| BsiHKAI | GWGCWC | 2 cut(s) | 152, 849 |
| BslFI | GGGAC | 2 cut(s) | 735, 1238 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1175 |
| BsmFI | GGGAC | 2 cut(s) | 735, 1238 |
| BsnI | GGCC | 2 cut(s) | 204, 322 |
| Bsp119I | TTCGAA | 1 cut(s) | 632 |
| Bsp1286I | GDGCHC | 2 cut(s) | 152, 849 |
| Bsp143I | GATC | 3 cut(s) | 400, 1021, 1199 |
| BspACI | CCGC | 1 cut(s) | 1048 |
| BspANI | GGCC | 2 cut(s) | 204, 322 |
| BspCNI | CTCAG | 3 cut(s) | 155, 1212, 1449 |
| BspFNI | CGCG | 1 cut(s) | 255 |
| BspLI | GGNNCC | 2 cut(s) | 306, 1226 |
| BspQI | GCTCTTC | 2 cut(s) | 854, 1290 |
| BspT104I | TTCGAA | 1 cut(s) | 632 |
| BspT107I | GGYRCC | 1 cut(s) | 304 |
| BspTI | CTTAAG | 1 cut(s) | 947 |
| BsrDI | GCAATG | 1 cut(s) | 508 |
| BsrI | ACTGG | 2 cut(s) | 598, 723 |
| BssECI | CCNNGG | 1 cut(s) | 814 |
| BssMI | GATC | 3 cut(s) | 400, 1021, 1199 |
| BssNAI | GTATAC | 1 cut(s) | 1399 |
| Bst1107I | GTATAC | 1 cut(s) | 1399 |
| Bst2UI | CCWGG | 2 cut(s) | 816, 1302 |
| Bst4CI | ACNGT | 1 cut(s) | 874 |
| Bst6I | CTCTTC | 2 cut(s) | 854, 1290 |
| BstAFI | CTTAAG | 1 cut(s) | 947 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1045 |
| BstBAI | YACGTR | 1 cut(s) | 983 |
| BstBI | TTCGAA | 1 cut(s) | 632 |
| BstC8I | GCNNGC | 1 cut(s) | 167 |
| BstDEI | CTNAG | 6 cut(s) | 142, 653, 785, 955, 1220, 1436 |
| BstF5I | GGATG | 1 cut(s) | 239 |
| BstFNI | CGCG | 1 cut(s) | 255 |
| BstKTI | GATC | 3 cut(s) | 403, 1024, 1202 |
| BstMBI | GATC | 3 cut(s) | 400, 1021, 1199 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 853, 1045, 1352 |
| BstNI | CCWGG | 2 cut(s) | 816, 1302 |
| BstNSI | RCATGY | 4 cut(s) | 11, 914, 1056, 1292 |
| BstSCI | CCNGG | 2 cut(s) | 814, 1300 |
| BstUI | CGCG | 1 cut(s) | 255 |
| BstV1I | GCAGC | 2 cut(s) | 919, 1032 |
| BstX2I | RGATCY | 1 cut(s) | 1199 |
| BstYI | RGATCY | 1 cut(s) | 1199 |
| BstZ17I | GTATAC | 1 cut(s) | 1399 |
| BsuRI | GGCC | 2 cut(s) | 204, 322 |
| BtgZI | GCGATG | 2 cut(s) | 448, 815 |
| BtsCI | GGATG | 1 cut(s) | 239 |
| BtsIMutI | CAGTG | 1 cut(s) | 1217 |
| Cac8I | GCNNGC | 1 cut(s) | 167 |
| Cfr13I | GGNCC | 2 cut(s) | 202, 1225 |
| Csp6I | GTAC | 5 cut(s) | 615, 657, 870, 892, 1253 |
| CviQI | GTAC | 5 cut(s) | 615, 657, 870, 892, 1253 |
| DdeI | CTNAG | 6 cut(s) | 142, 653, 785, 955, 1220, 1436 |
| DpnI | GATC | 3 cut(s) | 402, 1023, 1201 |
| DpnII | GATC | 3 cut(s) | 400, 1021, 1199 |
| DraI | TTTAAA | 2 cut(s) | 97, 1114 |
| EaeI | YGGCCR | 1 cut(s) | 320 |
| Eam1104I | CTCTTC | 2 cut(s) | 854, 1290 |
| EarI | CTCTTC | 2 cut(s) | 854, 1290 |
| Eco47I | GGWCC | 1 cut(s) | 1225 |
| EcoRI | GAATTC | 1 cut(s) | 184 |
| EcoRII | CCWGG | 2 cut(s) | 814, 1300 |
| EcoT22I | ATGCAT | 1 cut(s) | 1245 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 561, 593, 657, 689, 825, 857 |
| FaqI | GGGAC | 2 cut(s) | 735, 1238 |
| FbaI | TGATCA | 2 cut(s) | 400, 1021 |
| FblI | GTMKAC | 2 cut(s) | 934, 1398 |
| Fnu4HI | GCNGC | 2 cut(s) | 908, 1046 |
| FokI | GGATG | 1 cut(s) | 226 |
| Fsp4HI | GCNGC | 2 cut(s) | 908, 1046 |
| FspBI | CTAG | 7 cut(s) | 468, 582, 611, 768, 1067, 1094, 1148 |
| GluI | GCNGC | 2 cut(s) | 908, 1046 |
| GsaI | CCCAGC | 1 cut(s) | 203 |
| HaeIII | GGCC | 2 cut(s) | 204, 322 |
| HindIII | AAGCTT | 1 cut(s) | 534 |
| HinfI | GANTC | 5 cut(s) | 41, 191, 585, 607, 753 |
| HphI | GGTGA | 3 cut(s) | 321, 563, 1441 |
| Hpy166II | GTNNAC | 5 cut(s) | 175, 259, 825, 935, 1399 |
| Hpy188I | TCNGA | 5 cut(s) | 190, 367, 705, 1197, 1439 |
| Hpy8I | GTNNAC | 5 cut(s) | 175, 259, 825, 935, 1399 |
| HpyAV | CCTTC | 3 cut(s) | 541, 874, 905 |
| HpyCH4III | ACNGT | 1 cut(s) | 874 |
| HpyCH4IV | ACGT | 1 cut(s) | 982 |
| HpyCH4V | TGCA | 7 cut(s) | 604, 926, 1003, 1136, 1243, 1355, 1409 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 853, 1045, 1352 |
| HpyF3I | CTNAG | 6 cut(s) | 142, 653, 785, 955, 1220, 1436 |
| HpySE526I | ACGT | 1 cut(s) | 982 |
| Ksp22I | TGATCA | 2 cut(s) | 400, 1021 |
| Kzo9I | GATC | 3 cut(s) | 400, 1021, 1199 |
| LguI | GCTCTTC | 2 cut(s) | 854, 1290 |
| LmnI | GCTCC | 1 cut(s) | 706 |
| Lsp1109I | GCAGC | 2 cut(s) | 919, 1032 |
| LweI | GCATC | 1 cut(s) | 296 |
| MaeI | CTAG | 7 cut(s) | 468, 582, 611, 768, 1067, 1094, 1148 |
| MaeII | ACGT | 1 cut(s) | 982 |
| MaeIII | GTNAC | 3 cut(s) | 110, 133, 298 |
| MalI | GATC | 3 cut(s) | 402, 1023, 1201 |
| MboI | GATC | 3 cut(s) | 400, 1021, 1199 |
| MboII | GAAGA | 7 cut(s) | 90, 581, 841, 982, 1004, 1156, 1307 |
| MfeI | CAATTG | 1 cut(s) | 32 |
| MflI | RGATCY | 1 cut(s) | 1199 |
| MhlI | GDGCHC | 2 cut(s) | 152, 849 |
| MlsI | TGGCCA | 1 cut(s) | 322 |
| MluI | ACGCGT | 1 cut(s) | 253 |
| MluNI | TGGCCA | 1 cut(s) | 322 |
| MlyI | GAGTC | 2 cut(s) | 35, 616 |
| MmeI | TCCRAC | 1 cut(s) | 406 |
| MnlI | CCTC | 7 cut(s) | 41, 95, 253, 737, 1215, 1354, 1387 |
| Mox20I | TGGCCA | 1 cut(s) | 322 |
| Mph1103I | ATGCAT | 1 cut(s) | 1245 |
| MroXI | GAANNNNTTC | 1 cut(s) | 573 |
| MscI | TGGCCA | 1 cut(s) | 322 |
| MseI | TTAA | 4 cut(s) | 14, 96, 948, 1113 |
| MslI | CAYNNNNRTG | 1 cut(s) | 277 |
| Msp20I | TGGCCA | 1 cut(s) | 322 |
| MspCI | CTTAAG | 1 cut(s) | 947 |
| MspR9I | CCNGG | 2 cut(s) | 816, 1302 |
| MunI | CAATTG | 1 cut(s) | 32 |
| MvaI | CCWGG | 2 cut(s) | 816, 1302 |
| MvnI | CGCG | 1 cut(s) | 255 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 853, 1045, 1352 |
| NdeII | GATC | 3 cut(s) | 400, 1021, 1199 |
| NlaIV | GGNNCC | 2 cut(s) | 306, 1226 |
| NmuCI | GTSAC | 2 cut(s) | 133, 298 |
| NsiI | ATGCAT | 1 cut(s) | 1245 |
| NspI | RCATGY | 4 cut(s) | 11, 914, 1056, 1292 |
| NspV | TTCGAA | 1 cut(s) | 632 |
| PciI | ACATGT | 2 cut(s) | 7, 1288 |
| PciSI | GCTCTTC | 2 cut(s) | 854, 1290 |
| PdmI | GAANNNNTTC | 1 cut(s) | 573 |
| PfeI | GAWTC | 3 cut(s) | 191, 585, 753 |
| PkrI | GCNGC | 2 cut(s) | 909, 1047 |
| PleI | GAGTC | 2 cut(s) | 35, 615 |
| PpsI | GAGTC | 2 cut(s) | 35, 615 |
| Ppu21I | YACGTR | 1 cut(s) | 983 |
| PscI | ACATGT | 2 cut(s) | 7, 1288 |
| PsiI | TTATAA | 2 cut(s) | 1329, 1367 |
| Psp6I | CCWGG | 2 cut(s) | 814, 1300 |
| PspFI | CCCAGC | 1 cut(s) | 199 |
| PspGI | CCWGG | 2 cut(s) | 814, 1300 |
| PspN4I | GGNNCC | 2 cut(s) | 306, 1226 |
| PspPI | GGNCC | 2 cut(s) | 202, 1225 |
| PsuI | RGATCY | 1 cut(s) | 1199 |
| RsaI | GTAC | 5 cut(s) | 616, 658, 871, 893, 1254 |
| RsaNI | GTAC | 5 cut(s) | 615, 657, 870, 892, 1253 |
| RseI | CAYNNNNRTG | 1 cut(s) | 277 |
| SapI | GCTCTTC | 2 cut(s) | 854, 1290 |
| SaqAI | TTAA | 4 cut(s) | 14, 96, 948, 1113 |
| SatI | GCNGC | 2 cut(s) | 908, 1046 |
| Sau3AI | GATC | 3 cut(s) | 400, 1021, 1199 |
| Sau96I | GGNCC | 2 cut(s) | 202, 1225 |
| SchI | GAGTC | 2 cut(s) | 35, 616 |
| ScrFI | CCNGG | 2 cut(s) | 816, 1302 |
| SduI | GDGCHC | 2 cut(s) | 152, 849 |
| SfaNI | GCATC | 1 cut(s) | 296 |
| SfuI | TTCGAA | 1 cut(s) | 632 |
| SinI | GGWCC | 1 cut(s) | 1225 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 277 |
| SmlI | CTYRAG | 3 cut(s) | 43, 518, 947 |
| SmoI | CTYRAG | 3 cut(s) | 43, 518, 947 |
| SsiI | CCGC | 1 cut(s) | 1048 |
| SspMI | CTAG | 7 cut(s) | 468, 582, 611, 768, 1067, 1094, 1148 |
| StyD4I | CCNGG | 2 cut(s) | 814, 1300 |
| TaaI | ACNGT | 1 cut(s) | 874 |
| TaiI | ACGT | 1 cut(s) | 985 |
| TaqI | TCGA | 2 cut(s) | 632, 1382 |
| TatI | WGTACW | 1 cut(s) | 1252 |
| TfiI | GAWTC | 3 cut(s) | 191, 585, 753 |
| Tru1I | TTAA | 4 cut(s) | 14, 96, 948, 1113 |
| Tru9I | TTAA | 4 cut(s) | 14, 96, 948, 1113 |
| TscAI | CASTG | 1 cut(s) | 1224 |
| TseFI | GTSAC | 2 cut(s) | 133, 298 |
| TseI | GCWGC | 2 cut(s) | 907, 1045 |
| Tsp45I | GTSAC | 2 cut(s) | 133, 298 |
| TspDTI | ATGAA | 6 cut(s) | 303, 659, 723, 818, 1136, 1328 |
| TspRI | CASTG | 1 cut(s) | 1224 |
| Vha464I | CTTAAG | 1 cut(s) | 947 |
| VpaK11BI | GGWCC | 1 cut(s) | 1225 |
| XapI | RAATTY | 4 cut(s) | 184, 523, 780, 1185 |
| XbaI | TCTAGA | 4 cut(s) | 581, 1066, 1093, 1147 |
| XceI | RCATGY | 4 cut(s) | 11, 914, 1056, 1292 |
| XmiI | GTMKAC | 2 cut(s) | 934, 1398 |
| XmnI | GAANNNNTTC | 1 cut(s) | 573 |
| XspI | CTAG | 7 cut(s) | 468, 582, 611, 768, 1067, 1094, 1148 |
| Zsp2I | ATGCAT | 1 cut(s) | 1245 |