MD11G1032900.v1.1

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001306
Physical Location & Seq
Reverse (-)
2853678 .. 2857419
3742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1032900.v1.1.491

Sequence Viewer

Length: 1836 bp
ATGTCGATGGCCTCTAAATCCGGTGCCAGAAGTTCTTCCCCAGAGACTCAGAGGAAGTTAATTGCTAGTCAAACAACAGAAAGAAATTTCCGCAGACCTTCACCTCTTCCAGTTACAAAGACAAGCAAATCAGAGCCAGTCACTCCGAAAAAACTTCCTAGGCATGTGCAACAAGATGCGCCGAAGCATGTTTCTACAGAAACTATTGAAGAGAAGAACTGCAAGATTCCTTATCAGAAGGAGTCTGCCAAAACTTTAGCAGATCAGTTAGGTTCAGCTTTATCACTGGGTGATGCAAAGCAGGTGCCAGCTGATGTGGATCCTGTTGCGGGTGAGACTAAGAAGTTTCCAGGAGGCGGTGATAAAAAAAAGAAACCATCAGAAAATGGAATCAGCCCAACTTCAGCGAAAGTCAGTGATGATGGGACCAGCAGTATTGCCAAGACTAGTGGAAGCACCAAGATGAGTGGTCGAGCTGATTTTGTTGAGAGTGGGAAGAGCAGTATCTGTAGAGGCAGCACTAGCAGTGACATCAGTGATGAAAGTACTTGTAGCAGCTTTAGTAGTGCCATCAACAAACCTCACAAAGCAAATGACATACATTGGGAAGCAATTCAGGCTGTCCGATCAAGGGATGGTGTATTTGGTTTAGGCCATTTCAGACTTCTGAAGAAGCTGGGCTGTGGGGATATTGGAAGTGTCTATCTTTCAGAGTTGACCGGAACAAAATGTTATTTTGCAATGAAAGTTATGGACAAAGCATCTTTGGCTAACCGTAAAAAACTTCTTCGTGCTCAGACTGAGAGAGAAATACTTCAATGTTTAGACCATCCTTTCCTTCCAACCCTATATACCCATTTTGAGACAGAGAAGTATTCATGTCTGGTAATGGAGTTCTGCCCTGGAGGCGACTTGCACACACTTAGGCAAAGGCAACCCGGAAAGCATTTTACTGAGCAGGCAGTGAAGTTCTATGTAGCCGAGGTTCTCCTTGCACTGGAATATCTTCACATGCTTGGGATTGTTTATCGTGACCTTAAGCCGGAAAATGTCCTTGTGAGAGATGATGGGCACATAATGCTTTCCGACTTTGACCTTTCCCTGCGCTGTGCTGTCAGCCCAACCCTCGTCAAATCTTCAGCCCCTGACTCTGAACCTTTCCGAAGGAACTCAGCTTATTGTGTGCAACCTGCCTGCATTGAGCCCTCTTGTATTCAGCCATCTTGTGTGATGCCTACAACATGTTTCTCACCTCGTTTCTTTTCGAGTAAATCTAAGAAAGACCGGAAACCCAAGAATGAAATGGGGAACCAAGTACGCCCATTGCCAGAACTCATGGCTGAGCCAACCAATGCTCGTTCGATGTCATTTGTTGGGACCCATGAGTATTTGGCACCAGAAATTATCAAGGGTGAAGGGCATGGCAGTGCTGTTGACTGGTGGACCTTCGGGATCTTTCTGTATGAGCTGTTGTTTGGTAAAACACCTTTCAAGGGATCAGGAAATCGAGCCACCCTGTTCAACGTGGTAGGTCAGCCATTGAAATTCCCGGAAGCACCAGTGGTTAGCTTTTCAGCAAAGGATCTGATCAGGGGCCTACTTGTGAAAGAACCACAGCATAGGTTGGCATATAAGCGAGGAGCAACCGAGATTAAACAGCATCCGTTCTTTGAAGATGTTAATTGGGCATTGATTCGATGCGCAAGTCCTCCTGATATTCCAAAACCAGTCGAGTTTGAGCGGATTTCAGCTCCCCCAGTGTCAACCAGTGATAAAGCTACCGCTGCTGCTACTTATCCAGATCAAAACAATTATCTGGAATTCGATTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

612

Amino Acids

67.48

Weight (kDa)

8.93

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 221 - 370 6.8e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 221 - 370 7.1e-20 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 454 - 557 2.3e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 461 - 544 1e-05 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
Acc16I TGCGCA 1 cut(s) 1702
Acc36I ACCTGC 2 cut(s) 292, 1198
AccB1I GGYRCC 3 cut(s) 23, 304, 1393
AccBSI CCGCTC 1 cut(s) 1741
AciI CCGC 5 cut(s) 91, 329, 357, 1741, 1782
AclWI GGATC 5 cut(s) 314, 327, 1460, 1504, 1590
AcsI RAATTY 3 cut(s) 85, 1544, 1820
AcuI CTGAAG 3 cut(s) 387, 689, 1122
AdeI CACNNNGTG 1 cut(s) 290
AfaI GTAC 2 cut(s) 547, 1317
AfiI CCNNNNNNNGG 6 cut(s) 329, 356, 631, 997, 1042, 1493
AflII CTTAAG 1 cut(s) 1037
AflIII ACRYGT 1 cut(s) 1241
AgsI TTSAA 6 cut(s) 209, 818, 1492, 1522, 1543, 1673
AhlI ACTAGT 1 cut(s) 446
AjnI CCWGG 2 cut(s) 349, 901
Alw21I GWGCWC 1 cut(s) 796
Alw26I GTCTC 3 cut(s) 38, 329, 857
AlwI GGATC 5 cut(s) 314, 327, 1460, 1504, 1590
AlwNI CAGNNNCTG 2 cut(s) 507, 1145
AoxI GGCC 3 cut(s) 9, 652, 1594
ApeKI GCWGC 4 cut(s) 516, 555, 1784, 1787
ApoI RAATTY 3 cut(s) 85, 1544, 1820
Asp700I GAANNNNTTC 4 cut(s) 34, 612, 813, 1005
AspA2I CCTAGG 1 cut(s) 158
AspLEI GCGC 3 cut(s) 181, 1107, 1703
AspS9I GGNCC 4 cut(s) 426, 1377, 1443, 1594
AsuC2I CCSGG 2 cut(s) 939, 1550
AsuHPI GGTGA 6 cut(s) 93, 302, 344, 371, 1242, 1424
AvaII GGWCC 3 cut(s) 426, 1377, 1443
AvrII CCTAGG 1 cut(s) 158
BaeGI GKGCMC 1 cut(s) 1074
BamHI GGATCC 1 cut(s) 319
BanI GGYRCC 3 cut(s) 23, 304, 1393
BanII GRGCYC 1 cut(s) 1206
Bbv12I GWGCWC 1 cut(s) 796
BbvI GCAGC 4 cut(s) 528, 567, 1771, 1774
BccI CCATC 7 cut(s) 385, 416, 578, 629, 837, 1061, 1228
BciT130I CCWGG 2 cut(s) 351, 903
BclI TGATCA 1 cut(s) 1587
BcnI CCSGG 2 cut(s) 939, 1550
BcoDI GTCTC 3 cut(s) 38, 329, 857
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 66, 159, 447, 522
BfmI CTRYAG 2 cut(s) 195, 508
BfrI CTTAAG 1 cut(s) 1037
BfuAI ACCTGC 2 cut(s) 292, 1198
BglI GCCNNNNNGGC 1 cut(s) 906
BisI GCNGC 4 cut(s) 517, 556, 1785, 1788
BlnI CCTAGG 1 cut(s) 158
BlpI GCTNAGC 1 cut(s) 1341
BlsI GCNGC 4 cut(s) 518, 557, 1786, 1789
BmcAI AGTACT 1 cut(s) 547
Bme1390I CCNGG 4 cut(s) 351, 903, 939, 1550
Bme18I GGWCC 3 cut(s) 426, 1377, 1443
BmgT120I GGNCC 4 cut(s) 426, 1377, 1443, 1594
BmiI GGNNCC 9 cut(s) 25, 306, 321, 427, 1310, 1378, 1379, 1395, 1595
BmrFI CCNGG 4 cut(s) 351, 903, 939, 1550
BmrI ACTGGG 2 cut(s) 296, 1751
BmsI GCATC 6 cut(s) 166, 283, 770, 1221, 1669, 1688
BmuI ACTGGG 2 cut(s) 296, 1751
BpmI CTGGAG 1 cut(s) 924
Bpu1102I GCTNAGC 1 cut(s) 1341
BpuMI CCSGG 2 cut(s) 939, 1550
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 3 cut(s) 158, 901, 981
BsaWI WCCGGW 3 cut(s) 20, 719, 1284
Bsc4I CCNNNNNNNGG 6 cut(s) 329, 356, 631, 997, 1042, 1493
Bse1I ACTGG 9 cut(s) 110, 137, 291, 1002, 1442, 1559, 1727, 1757, 1767
Bse3DI GCAATG 2 cut(s) 747, 1322
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 2 cut(s) 351, 903
BseDI CCNNGG 3 cut(s) 158, 901, 981
BseGI GGATG 3 cut(s) 640, 829, 1660
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 6 cut(s) 329, 356, 631, 997, 1042, 1493
BseMI GCAATG 2 cut(s) 747, 1322
BseMII CTCAG 6 cut(s) 62, 792, 809, 945, 1185, 1332
BseNI ACTGG 9 cut(s) 110, 137, 291, 1002, 1442, 1559, 1727, 1757, 1767
BseRI GAGGAG 1 cut(s) 1653
BseSI GKGCMC 1 cut(s) 1074
BseXI GCAGC 4 cut(s) 528, 567, 1771, 1774
BseYI CCCAGC 1 cut(s) 676
BshFI GGCC 3 cut(s) 11, 654, 1596
BshNI GGYRCC 3 cut(s) 23, 304, 1393
BsiHKAI GWGCWC 1 cut(s) 796
BsiSI CCGG 6 cut(s) 21, 720, 939, 1043, 1285, 1550
BslFI GGGAC 2 cut(s) 439, 1390
BslI CCNNNNNNNGG 6 cut(s) 329, 356, 631, 997, 1042, 1493
BsmAI GTCTC 3 cut(s) 38, 329, 857
BsmFI GGGAC 2 cut(s) 439, 1390
BsnI GGCC 3 cut(s) 11, 654, 1596
Bsp1286I GDGCHC 3 cut(s) 796, 1074, 1206
Bsp143I GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
Bsp1720I GCTNAGC 1 cut(s) 1341
BspACI CCGC 5 cut(s) 91, 329, 357, 1741, 1782
BspANI GGCC 3 cut(s) 11, 654, 1596
BspCNI CTCAG 6 cut(s) 61, 793, 808, 946, 1184, 1333
BspLI GGNNCC 9 cut(s) 25, 306, 321, 427, 1310, 1378, 1379, 1395, 1595
BspMI ACCTGC 2 cut(s) 292, 1198
BspPI GGATC 5 cut(s) 314, 327, 1460, 1504, 1590
BspQI GCTCTTC 1 cut(s) 491
BspT107I GGYRCC 3 cut(s) 23, 304, 1393
BspTI CTTAAG 1 cut(s) 1037
BsrBI CCGCTC 1 cut(s) 1741
BsrDI GCAATG 2 cut(s) 747, 1322
BsrI ACTGG 9 cut(s) 110, 137, 291, 1002, 1442, 1559, 1727, 1757, 1767
BssECI CCNNGG 3 cut(s) 158, 901, 981
BssMI GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 2 cut(s) 351, 903
Bst4CI ACNGT 1 cut(s) 776
Bst6I CTCTTC 3 cut(s) 111, 204, 491
BstAFI CTTAAG 1 cut(s) 1037
BstAPI GCANNNNNTGC 1 cut(s) 1078
BstC8I GCNNGC 3 cut(s) 309, 960, 1195
BstDEI CTNAG 9 cut(s) 48, 339, 795, 801, 923, 954, 1171, 1275, 1341
BstF5I GGATG 3 cut(s) 640, 829, 1660
BstHHI GCGC 3 cut(s) 181, 1107, 1703
BstKTI GATC 8 cut(s) 265, 322, 629, 1455, 1499, 1585, 1590, 1804
BstMAI GTCTC 3 cut(s) 38, 329, 857
BstMBI GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
BstMWI GCNNNNNNNGC 6 cut(s) 522, 617, 767, 906, 1078, 1784
BstNI CCWGG 2 cut(s) 351, 903
BstNSI RCATGY 4 cut(s) 167, 191, 1015, 1245
BstSCI CCNGG 4 cut(s) 349, 901, 937, 1548
BstSFI CTRYAG 2 cut(s) 195, 508
BstSLI GKGCMC 1 cut(s) 1074
BstV1I GCAGC 4 cut(s) 528, 567, 1771, 1774
BstX2I RGATCY 3 cut(s) 319, 1452, 1582
BstYI RGATCY 3 cut(s) 319, 1452, 1582
BsuRI GGCC 3 cut(s) 11, 654, 1596
BtsCI GGATG 3 cut(s) 640, 829, 1660
BtsI GCAGTG 3 cut(s) 532, 969, 1432
BveI ACCTGC 2 cut(s) 292, 1198
Cac8I GCNNGC 3 cut(s) 309, 960, 1195
CaiI CAGNNNCTG 2 cut(s) 507, 1145
CfoI GCGC 3 cut(s) 181, 1107, 1703
Cfr13I GGNCC 4 cut(s) 426, 1377, 1443, 1594
Csp6I GTAC 2 cut(s) 546, 1316
CspCI CAANNNNNGTGG 4 cut(s) 430, 448, 465, 483
CviAII CATG 8 cut(s) 164, 188, 879, 1012, 1242, 1336, 1382, 1421
CviQI GTAC 2 cut(s) 546, 1316
DdeI CTNAG 9 cut(s) 48, 339, 795, 801, 923, 954, 1171, 1275, 1341
DpnI GATC 8 cut(s) 264, 321, 628, 1454, 1498, 1584, 1589, 1803
DpnII GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
DraIII CACNNNGTG 1 cut(s) 290
Eam1104I CTCTTC 3 cut(s) 111, 204, 491
EarI CTCTTC 3 cut(s) 111, 204, 491
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 1206
Eco47I GGWCC 3 cut(s) 426, 1377, 1443
Eco57I CTGAAG 3 cut(s) 387, 689, 1122
EcoO109I RGGNCCY 2 cut(s) 1377, 1594
EcoRI GAATTC 1 cut(s) 1820
EcoRII CCWGG 2 cut(s) 349, 901
EcoT14I CCWWGG 1 cut(s) 158
EcoT38I GRGCYC 1 cut(s) 1206
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 8 cut(s) 167, 191, 882, 1015, 1245, 1339, 1385, 1424
FaqI GGGAC 2 cut(s) 439, 1390
FatI CATG 8 cut(s) 163, 187, 878, 1011, 1241, 1335, 1381, 1420
FauI CCCGC 1 cut(s) 322
FbaI TGATCA 1 cut(s) 1587
Fnu4HI GCNGC 4 cut(s) 517, 556, 1785, 1788
FokI GGATG 3 cut(s) 647, 816, 1647
FriOI GRGCYC 1 cut(s) 1206
Fsp4HI GCNGC 4 cut(s) 517, 556, 1785, 1788
FspBI CTAG 4 cut(s) 66, 159, 447, 522
FspI TGCGCA 1 cut(s) 1702
GlaI GCGC 3 cut(s) 180, 1106, 1702
GluI GCNGC 4 cut(s) 517, 556, 1785, 1788
GsaI CCCAGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 3 cut(s) 11, 654, 1596
HapII CCGG 6 cut(s) 21, 720, 939, 1043, 1285, 1550
HhaI GCGC 3 cut(s) 181, 1107, 1703
Hin1II CATG 8 cut(s) 167, 191, 882, 1015, 1245, 1339, 1385, 1424
Hin6I GCGC 3 cut(s) 179, 1105, 1701
HinP1I GCGC 3 cut(s) 179, 1105, 1701
HincII GTYRAC 3 cut(s) 717, 1435, 1764
HindII GTYRAC 3 cut(s) 717, 1435, 1764
HinfI GANTC 6 cut(s) 46, 226, 242, 390, 1148, 1693
HpaII CCGG 6 cut(s) 21, 720, 939, 1043, 1285, 1550
HphI GGTGA 6 cut(s) 93, 302, 344, 371, 1242, 1424
Hpy166II GTNNAC 4 cut(s) 717, 1435, 1443, 1764
Hpy188III TCNNGA 6 cut(s) 1031, 1450, 1500, 1712, 1799, 1817
Hpy8I GTNNAC 4 cut(s) 717, 1435, 1443, 1764
HpyAV CCTTC 6 cut(s) 108, 232, 848, 1158, 1409, 1456
HpyCH4III ACNGT 1 cut(s) 776
HpyCH4IV ACGT 1 cut(s) 1524
HpyCH4V TGCA 8 cut(s) 169, 222, 296, 740, 916, 995, 1186, 1197
HpyF10VI GCNNNNNNNGC 6 cut(s) 522, 617, 767, 906, 1078, 1784
HpyF3I CTNAG 9 cut(s) 48, 339, 795, 801, 923, 954, 1171, 1275, 1341
HpySE526I ACGT 1 cut(s) 1524
Hsp92II CATG 8 cut(s) 167, 191, 882, 1015, 1245, 1339, 1385, 1424
HspAI GCGC 3 cut(s) 179, 1105, 1701
KflI GGGWCCC 1 cut(s) 1377
Ksp22I TGATCA 1 cut(s) 1587
Kzo9I GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
LguI GCTCTTC 1 cut(s) 491
LmnI GCTCC 2 cut(s) 1640, 1756
Lsp1109I GCAGC 4 cut(s) 528, 567, 1771, 1774
LweI GCATC 6 cut(s) 166, 283, 770, 1221, 1669, 1688
MaeI CTAG 4 cut(s) 66, 159, 447, 522
MaeII ACGT 1 cut(s) 1524
MaeIII GTNAC 4 cut(s) 112, 139, 527, 1031
MalI GATC 8 cut(s) 264, 321, 628, 1454, 1498, 1584, 1589, 1803
MbiI CCGCTC 1 cut(s) 1741
MboI GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
MflI RGATCY 3 cut(s) 319, 1452, 1582
MhlI GDGCHC 3 cut(s) 796, 1074, 1206
MluCI AATT 8 cut(s) 60, 85, 612, 1401, 1544, 1681, 1810, 1820
MlyI GAGTC 3 cut(s) 40, 251, 1142
MmeI TCCRAC 2 cut(s) 866, 1110
MroXI GAANNNNTTC 4 cut(s) 34, 612, 813, 1005
MseI TTAA 4 cut(s) 59, 1038, 1653, 1680
MslI CAYNNNNRTG 2 cut(s) 461, 1425
MspA1I CMGCKG 2 cut(s) 311, 1784
MspCI CTTAAG 1 cut(s) 1037
MspI CCGG 6 cut(s) 21, 720, 939, 1043, 1285, 1550
MspR9I CCNGG 4 cut(s) 351, 903, 939, 1550
MvaI CCWGG 2 cut(s) 351, 903
MwoI GCNNNNNNNGC 6 cut(s) 522, 617, 767, 906, 1078, 1784
NciI CCSGG 2 cut(s) 939, 1550
NdeII GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
NlaIII CATG 8 cut(s) 167, 191, 882, 1015, 1245, 1339, 1385, 1424
NlaIV GGNNCC 9 cut(s) 25, 306, 321, 427, 1310, 1378, 1379, 1395, 1595
NmeAIII GCCGAG 1 cut(s) 1006
NmuCI GTSAC 3 cut(s) 139, 527, 1031
NsbI TGCGCA 1 cut(s) 1702
NspI RCATGY 4 cut(s) 167, 191, 1015, 1245
PaqCI CACCTGC 1 cut(s) 292
PciI ACATGT 1 cut(s) 1241
PciSI GCTCTTC 1 cut(s) 491
PdmI GAANNNNTTC 4 cut(s) 34, 612, 813, 1005
PfeI GAWTC 3 cut(s) 226, 390, 1693
PfoI TCCNGGA 2 cut(s) 349, 1548
PkrI GCNGC 4 cut(s) 518, 557, 1786, 1789
PleI GAGTC 3 cut(s) 40, 250, 1142
PpsI GAGTC 3 cut(s) 40, 250, 1142
PpuMI RGGWCCY 1 cut(s) 1377
PscI ACATGT 1 cut(s) 1241
Psp5II RGGWCCY 1 cut(s) 1377
Psp6I CCWGG 2 cut(s) 349, 901
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 2 cut(s) 349, 901
PspN4I GGNNCC 9 cut(s) 25, 306, 321, 427, 1310, 1378, 1379, 1395, 1595
PspPI GGNCC 4 cut(s) 426, 1377, 1443, 1594
PspPPI RGGWCCY 1 cut(s) 1377
PsrI GAACNNNNNNTAC 2 cut(s) 969, 1001
PstNI CAGNNNCTG 2 cut(s) 507, 1145
PsuI RGATCY 3 cut(s) 319, 1452, 1582
PvuII CAGCTG 1 cut(s) 311
RsaI GTAC 2 cut(s) 547, 1317
RsaNI GTAC 2 cut(s) 546, 1316
RseI CAYNNNNRTG 2 cut(s) 461, 1425
SapI GCTCTTC 1 cut(s) 491
SaqAI TTAA 4 cut(s) 59, 1038, 1653, 1680
SatI GCNGC 4 cut(s) 517, 556, 1785, 1788
Sau3AI GATC 8 cut(s) 262, 319, 626, 1452, 1496, 1582, 1587, 1801
Sau96I GGNCC 4 cut(s) 426, 1377, 1443, 1594
ScaI AGTACT 1 cut(s) 547
SchI GAGTC 3 cut(s) 40, 251, 1142
ScrFI CCNGG 4 cut(s) 351, 903, 939, 1550
SduI GDGCHC 3 cut(s) 796, 1074, 1206
SfaNI GCATC 6 cut(s) 166, 283, 770, 1221, 1669, 1688
SfcI CTRYAG 2 cut(s) 195, 508
SinI GGWCC 3 cut(s) 426, 1377, 1443
SmiMI CAYNNNNRTG 2 cut(s) 461, 1425
SmlI CTYRAG 1 cut(s) 1037
SmoI CTYRAG 1 cut(s) 1037
SpeI ACTAGT 1 cut(s) 446
Sse9I AATT 8 cut(s) 60, 85, 612, 1401, 1544, 1681, 1810, 1820
SsiI CCGC 5 cut(s) 91, 329, 357, 1741, 1782
SspMI CTAG 4 cut(s) 66, 159, 447, 522
StyD4I CCNGG 4 cut(s) 349, 901, 937, 1548
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 1 cut(s) 776
TaiI ACGT 1 cut(s) 1527
TaqI TCGA 8 cut(s) 5, 472, 1265, 1361, 1507, 1696, 1731, 1824
TasI AATT 8 cut(s) 60, 85, 612, 1401, 1544, 1681, 1810, 1820
TatI WGTACW 1 cut(s) 545
TfiI GAWTC 3 cut(s) 226, 390, 1693
Tru1I TTAA 4 cut(s) 59, 1038, 1653, 1680
Tru9I TTAA 4 cut(s) 59, 1038, 1653, 1680
TseFI GTSAC 3 cut(s) 139, 527, 1031
TseI GCWGC 4 cut(s) 516, 555, 1784, 1787
Tsp45I GTSAC 3 cut(s) 139, 527, 1031
TspDTI ATGAA 4 cut(s) 555, 758, 867, 1314
TspGWI ACGGA 1 cut(s) 1653
Vha464I CTTAAG 1 cut(s) 1037
VpaK11BI GGWCC 3 cut(s) 426, 1377, 1443
XapI RAATTY 3 cut(s) 85, 1544, 1820
XceI RCATGY 4 cut(s) 167, 191, 1015, 1245
XcmI CCANNNNNNNNNTGG 1 cut(s) 1300
XmaJI CCTAGG 1 cut(s) 158
XmnI GAANNNNTTC 4 cut(s) 34, 612, 813, 1005
XspI CTAG 4 cut(s) 66, 159, 447, 522
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.